CN105445404A - Corn oil authentication method adopting stable carbon isotope technique - Google Patents
Corn oil authentication method adopting stable carbon isotope technique Download PDFInfo
- Publication number
- CN105445404A CN105445404A CN201510822043.2A CN201510822043A CN105445404A CN 105445404 A CN105445404 A CN 105445404A CN 201510822043 A CN201510822043 A CN 201510822043A CN 105445404 A CN105445404 A CN 105445404A
- Authority
- CN
- China
- Prior art keywords
- corn oil
- glycerine
- carbon isotope
- oil
- solution
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
Landscapes
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Fats And Perfumes (AREA)
- Edible Oils And Fats (AREA)
Abstract
The invention discloses an authentication method for edible corn oil and particularly provides a corn oil authentication method adopting a stable carbon isotope technique. The corn oil authentication method is established according to a natural fractionation characteristic of the stable carbon isotope, a plant anabolism theory, a transesterification technology and the stable carbon isotope analysis technique. The distribution range of delta<13>C values of glycerin of the corn oil is established through determination of the delta<13>C values of glycerin of triglyceride in grease, and the authenticity of a to-be-detected sample is analyzed according to the principle of statistics. The corn oil authentication method is established by adopting the technique for analyzing the stable carbon isotope of the glycerin in the grease, the technical problem for detection of illegal addition of low-priced grease to the corn oil existing in the edible oil market in China is solved, the consumer benefit is protected, and healthy and ordered development of the edible oil market is promoted.
Description
Technical field:
The invention belongs to edible oil quality detection field.Be specifically related to a kind of method utilizing stable carbon isotope technique to differentiate the corn oil true and false.
Background technology:
Edible vegetable oil is daily life indispensable requisites, and price variance is very large because its nutritive value is different for different types of vegetable oil.Maize germ is the grease that the northern people generally eat, but in recent years because grain purchase and sale market is decontroled comprehensively, the grease in the palm source that price is lower and other lipid phase become some illegal retailers and pursue the adulterated target of interests, mix in corn oil by cheap grease, the event of palming off the sale of pure corn oil happens occasionally.
The True-false distinguish of edible vegetable oil is the technical barrier that countries in the world all face, receive the concern of various countries scientific worker and establish a large amount of analytical approachs, current spectral technique, gas chromatographic technique, nuclear magnetic resonance technique, Electronic Nose Technology, electrochemical techniques and round pcr are the emphasis of current research, but do not occur that one is comparatively general so far yet, the gratifying authentication technique of effect, although this is mainly because different greases may have different one-tenth and be grouped into, even if but sterling grease, it becomes to be grouped into neither be changeless, due to the place of production, the condition impacts such as weather, its major component and content are also not quite similar.According to (DeterminationofStableIsotopeRatiosinFoodAnalysis), (TheapplicationofNMRandMSmethodsfordetectionofadulteratio nofwine, fruitjuices, andoliveoil.Areview), (CharacterizationofOliveOilbyCarbonIsotopeAnalysisofIndiv idualFattyAcids:ImplicationsforAuthentication), (Applicationofstableisotoperatioanalysistothecharacteriza tionofthegeographicaloriginofoliveoils), (StableIsotopeCharacterizationofOliveOils.I-CompositionalandCarbon-13ProfilesofFattyAcids), (IsotopicandelementaldatafortracingtheoriginofEuropeanoli veoils), (Authenticationofvegetableoilsbybulkandmolecularcarboniso topeanalyseswithemphasisonoliveoilandpumpkinseedoil), (CharacterisationofauthenticItalianextra-virginoliveoilsb ystableisotoperatiosofC, OandHandmineralcomposition), (Characterizationofvirginoliveoilsaccordingtoitstriglycer idesandsterolscompositionbychemometricmethods), (PurityAssessmentsofMajorVegetableOilsBasedon δ 13CValuesofIndividualFattyAcids) reports, stable isotope is differentiated and place of production discriminating in the puppet of mixing of vegetable oil (especially olive oil), but it is less for the research report of grease cheap in corn oil.The domestic research in this field is started late, and only has the people such as Jin Qingzhe to utilize palm oil in the isotopic Difference test corn oil of fatty acid carbons in grease so far, the people such as Guo Lianxian adopt the carbon isotope composition of triglyceride in grease and the carbon isotope composition of fatty acid to carry out the Adulteration detection research of vegetable oil simultaneously, but there is certain drawback in this technology: triglyceride is by glycerine and Fatty acid compositions, but the contamination of fatty acid has larger difference in the grease of different cultivars, even and same kind, when the place of production is different, content of fatty acid is also had any different, and according to the principle of Isotope metabolism fractionation, the chemical reaction difference of different compounds owing to relating in bio-metabolic process, its carbon isotope composition is also different, therefore, the carbon isotope composition of triglyceride is difficult to mix the pseudo-technical indicator differentiated as grease, the carbon isotope composition of fatty acid is the important technology index of current Adulteration detection research and apply, but, because the content of fatty acid of the grease of different cultivars is different, just not simply during fake producer's manufacturing and marketing of counterfeit and substandard goods in high price oil, mix cheap grease, also can add the material such as essence, verdigris element, make the kind of fatty acid and content consistent with sterling oil as far as possible, in this case, the carbon isotope composition of fatty acid can be subject to larger interference in application process, causes misjudgment.Glycerine is the important component part in grease, constitutes " skeleton " of triglyceride.The grease in each kind, each source, regardless of its fatty acid composition, the ratio of its glycerine is almost fixing, and therefore, in research triglyceride, the Carbon Isotope Characteristics of glycerine is more meaningful.
Summary of the invention
The object of the present invention is to provide a kind of method effectively differentiating the corn oil true and false based on glycerine stable carbon isotope technique, thus solve the corn oil quality detection technology difficult problem that current China edible oil market exists, normalize market behavior, Protection of consumer rights and interests.
The object of the invention is to be achieved through the following technical solutions:
The present invention relates to a kind of method utilizing carbon isotope technique to differentiate the corn oil true and false, described method comprises the δ that employing gas chromatography-burning-Stable isotope ratio mass spectrometer measures the glycerin component of grease
13c, sets up the δ of glycerin component in known real corn oil
13c distribution range, then analyzes the glycerine δ of testing sample
13c value also carries out significant difference analysis with the distribution range of authentic sample, if there is not significant difference analysis, shows testing sample not containing non-corn oil grease, otherwise then shows in testing sample containing cheap grease instead of real corn oil.Preferably, in the practice of the invention, can build glycerine in corn source grease carbon isotopic data storehouse or select glycerine carbon isotope endogenous mark (
13c-InternalReferenceMaterial), thus when using the present invention to identify corn oil, do not need all to measure δ in the glycerine from the corn oil in the same place of production at every turn
13c, but the glycerine carbon isotopic data storehouse or the endogenous mark that use the corn oil set up.
Preferably, known real corn oil sample of the present invention is that what to obtain from corn oil manufacturing enterprise take corn as the authentic sample of corn oil that raw material is produced, and the grease carrying out physical squeezing process to corn under laboratory condition and obtain.
Testing sample of the present invention can be the sample being labeled as corn oil commercially.
The glycerine δ of known real corn oil sample of the present invention
13c database builds by following method: collected specimens, wherein known real corn oil finished product more than 20, and more than 10 parts, maize raw material sample, then measures δ in the glycerine of corn oil
13c value, sets up the glycerine δ of corn oil
13c database, wherein adopts statistical method to analyze the glycerine δ of corn oil
13effective distribution range of C.
In the present invention corn oil glycerine in δ
13c assay method is that oil sample measures with gas chromatography-burning-Stable isotope ratio mass spectrometer after treatment, chromatographic condition is as follows: quartz capillary chromatographic column, preferred polarity chromatographic column (as Wax chromatographic column, specification is 50m*0.25mm*0.20um); Carrier gas is high-purity helium (if purity is higher than 99.999%); Adopt constant current mode (as flowing water is set to 1.2mL/min); Column temperature adopts temperature programme: initial temperature 120 DEG C, keeps 1min, rises to 220 DEG C with the speed of 20 DEG C/min, keeps 7min; Be equipped with flame tube in combustion reaction process, flame tube material is pottery, inner filling cupric oxide (improving the oxidisability of flame tube if desired with oxygen), and working temperature is set as 1000 DEG C; Sample size is 1 microlitre.Preferably one, glycerine δ
13c first adjusts Instrument working state before measuring, and ensures that carbon isotope analysis is stablized; Preferably two, the glycerine δ of oil sample
13δ should be used before and after C pH-value determination pH
13the glycerine reference material that C value is known or working stamndard carry out analysis correction, to ensure the accuracy that carbon isotope measures.
Oil sample glycerine δ in the present invention
13the pre-treatment step needed before C pH-value determination pH is as follows:
A) getting edible oil sample is dissolved in isooctane, fully shakes;
B) in above-mentioned solution, add potassium hydroxide methanol solution, fully leave standstill after concussion, obtain lower floor settled solution b;
C) acidifying b solution, adds isooctane, and fatty acid residual in removing solution, obtains mixed solution c;
D) adjusting the solution obtained in c is about 7 to pH, and adds absolute methanol solution, obtains glycerine methanol solution d, stand-by;
Compared with prior art, the present invention has following beneficial effect:
1. the glycerine δ of the ground-breaking discovery corn oil of the present invention
13the grease of C value and other kinds has notable difference, and the glycerine of grease more can the carbon isotope distribution characteristics of direct, clear display grease, and not easily fakes, and is disturbed less;
2. the ground-breaking δ analyzing glycerine in grease in conjunction with ester-exchange technology and gas phase chromatogram-burning-Stable isotope ratio mass-spectrometric technique of the present invention
13c value, method is simple, and easy to operate, data are reliable.
3. method of the present invention is simple to operate, and cost is low, effectively can solve the True-false distinguish problem of corn oil, for safeguarding consumers' rights and interests, promotes that the sound development of China edible oil market is significant.
Accompanying drawing illustrates:
Accompanying drawing 1 gas chromatography-burning-Stable isotope ratio mass-spectrometric technique measures glycerine δ in grease
1344,45,46 ion flow graphs of C value.
Embodiment
Embodiment 1
δ in the glycerine of grease
13the mensuration of C value
1.1 sample pretreatment
Take 0.12g vegetable oil and be dissolved in 8mL isooctane; Add 400 μ L supersaturation potassium hydroxide methanol solutions, abundant vibration leaves standstill afterwards and removes upper solution, to add in lower floor's solution in about 70 μ L concentrated hydrochloric acids and potassium hydroxide keep acid, solution layering removes upper solution (mainly free fatty acid) again, add in 50 μ L supersaturation potassium hydroxide methanol solutions in lower floor's solution and concentrated hydrochloric acid, after salt precipitation, add 1mL methyl alcohol, and the methyl alcohol upper solution containing glycerine is transferred in 2mL reagent bottle, stand-by.
1.2 chromatographic condition
CP-Wax capillary chromatographic column (25m*0.25mm*0.20 μm); Carrier gas is helium; Column flow rate 1.2mL/min; Injector temperature 270 DEG C; Heating schedule is: initial temperature 120 DEG C, keeps 1min, is warming up to 220 DEG C with 20 DEG C/min, keeps 7min; Sampling volume 1 μ L; Split ratio 20:1.
1.3 burner conditions of work
Use flame tube (ceramic pipe, inner filling cupric oxide) in burner, working temperature is 1000 DEG C.
1.4 instrument performance inspections
Inspection Stable isotope ratio mass spectrometer measures δ
13the interior precision of C value and linear analysis scope, wherein in precision SD should be not more than 0.06 ‰, when ion current intensity is 2V ~ 9V analytical error be not more than ± 0.066 ‰.
1.5 measure
The δ of glycerine is measured in the glycerite injection gas chromatography-burning-Stable isotope ratio mass spectrometer obtained through pre-service by oil sample
13c value, is shown in accompanying drawing 1.
1.6 calibration of the output results
The glycerine δ of oil sample
13all δ is measured before and after C pH-value determination pH
13the glycerine working standard that C value is known, and the glycerine δ correcting oil sample with this working standard
13c pH-value determination pH result.
Embodiment 2
Example differentiated by corn oil sample
2.1 collect respectively Different sources, 20, corn oil sample that authenticity is known, 10, the maize raw material sample of Different sources, maize raw material sample physical squeezing under laboratory condition goes out grease; 2.2 collect each one known of sunflower oil, rapeseed oil, soybean oil, peanut oil, olive oil and animal fat sample of authenticity;
2.3 3, the samples being commercially labeled as corn oil;
δ in the glycerine of 2.4 greases
13c pH-value determination pH:
2.4.1 sample pretreatment
Take 0.12g vegetable oil and be dissolved in 8mL isooctane; Add 400 μ L supersaturation potassium hydroxide methanol solutions, abundant vibration leaves standstill afterwards and removes upper solution, to add in lower floor's solution in about 70 μ L concentrated hydrochloric acids and potassium hydroxide keep acid, solution layering removes upper solution (mainly free fatty acid) again, add in 50 μ L supersaturation potassium hydroxide methanol solutions in lower floor's solution and concentrated hydrochloric acid, after salt precipitation, add 1mL methyl alcohol, and the methyl alcohol upper solution containing glycerine is transferred in 2mL reagent bottle, stand-by.
2.4.2 chromatographic condition
CP-Wax capillary chromatographic column (25m*0.25mm*0.20 μm); Carrier gas is helium; Column flow rate 1.2mL/min; Injector temperature 270 DEG C; Heating schedule is: initial temperature 120 DEG C, keeps 1min, is warming up to 220 DEG C with 20 DEG C/min, keeps 7min; Sampling volume 1 μ L; Split ratio 20:1.
2.4.3 use flame tube (ceramic pipe, inner filling cupric oxide) in burner condition of work burner, working temperature is 1000 DEG C.
2.4.4 instrument performance inspection
Inspection Stable isotope ratio mass spectrometer measures δ
13the interior precision of C value and linear analysis scope, wherein in precision SD should be not more than 0.06 ‰, when ion current intensity is 2V ~ 9V analytical error be not more than ± 0.066 ‰.
2.4.5 measure
The δ of glycerine is measured in the glycerite injection gas chromatography-burning-Stable isotope ratio mass spectrometer obtained through pre-service by oil sample
13c value, is shown in accompanying drawing 1.
2.4.6 calibration of the output results
The glycerine δ of oil sample
13all δ is measured before and after C pH-value determination pH
13the glycerine working standard that C value is known, and the glycerine δ correcting oil sample with this working standard
13c pH-value determination pH result.
2.7 interpretation of result
2.7.1 corn oil sample
The glycerine δ of the grease of the corn oil sample that authenticity is known and the squeezing of maize raw material laboratory
13c scope sees the following form 1.
The glycerine δ of table 1 corn oil and corn squeezing grease
13c measures descriptive statistics
As shown in Table 1, the δ of the corn oil sample that authenticity is known
13c distribution range is-13.25 ‰ ~-15.78 ‰, and maize raw material is-14.16 ‰ ~-15.65 ‰ through the grease of laboratory physical squeezing, the glycerine δ of two class corn oils
13the mean value of C is respectively-14.28 ‰ and-14.87 ‰, and this illustrates δ in the glycerine of the corn oil of suitability for industrialized production and the corn grease of laboratory condition squeezing
13c value is almost consistent, and the glycerine δ of two class corn oils
13the standard deviation of C value is all less than 0.7 ‰, and this illustrates the δ of corn oil glycerine
13the undulatory property of C value is less, and the impact by conditions such as the places of production is less.
2.7.2 non-corn oil sample
The glycerine δ of sunflower oil, rapeseed oil, soybean oil, peanut oil, olive oil and animal fat sample
13c value is in table 2.
The glycerine δ of the non-corn oil sample of table 2
13c measures descriptive statistics
| Fat type | δ 13C(‰) |
| Sunflower oil | -31.35 |
| Rapeseed oil | -32.26 |
| Soybean oil | -32.30 |
| Peanut oil | -32.07 |
| Olive oil | -31.13 |
| Palm oil | -29.75 |
| Animal tallow | -22.15 |
As shown in Table 2, δ in the glycerine of non-corn oil sample and corn oil
13c value significant difference, detail does not belong to a class, wherein the differing greatly of sunflower oil, rapeseed oil, soybean oil, peanut oil, olive oil and palm oil and corn oil, and minimum is 13.07 ‰, maximumly reaches 19.05; The mean difference of animal tallow and corn oil is 7.57 ‰, thus can according to δ in the glycerine of grease
13whether C value carrys out judgement sample is corn oil.
2.7.3 testing sample
The descriptive statistic of testing sample is in table 3.
The glycerine δ of table 3 measuring samples
13c measures descriptive statistics
| Numbering | δ 13C(‰) |
| 1# | -15.47 |
| 2# | -16.21 |
| 3# | -19.55 |
As shown in Table 3, the glycerine δ of 1# sample
13c value, in the distribution range (-13.25 ‰ ~-15.78 ‰) of corn oil, can be considered pure corn oil; The δ of 2# sample
13although C value is not in the scope of this paper institute building database, fractionation feature due to stable isotope is subject to the impact of the conditions such as the starting material place of production, and database sample size is herein on the low side, really all samples can not may be contained, therefore adopt statistical analysis method to judge, the fiducial interval according to 95% can think that this sample is pure corn oil; The δ of 3# sample
13c value not only not within the scope of database, nor belongs to the fiducial interval of 95%, therefore assert that this sample is not pure corn oil, but is mixed with the grease of certain other kinds.
Finally should be noted that, above embodiment is only unrestricted for illustration of technical scheme of the present invention, although with reference to preferred embodiment to invention has been detailed description, those of ordinary skill in the art is to be understood that, can modify to the technical scheme of invention or equivalent replacement, and not departing from the spirit and scope of technical solution of the present invention, it all should be encompassed in right of the present invention.
Claims (8)
1. utilize carbon isotope technique to differentiate a method for the corn oil true and false, it is characterized in that, measure the δ of known real corn oil glycerin component
13c, sets up the δ of glycerin component in known real corn oil
13c distribution range, then analyzes the glycerine δ of testing sample
13c value also carries out significant difference analysis with the distribution range of authentic sample, if there is not significant difference analysis, shows testing sample not containing non-corn oil grease, otherwise then shows in testing sample containing cheap grease instead of real corn oil.
2. the carbon isotope technique that utilizes according to claim 1 differentiates corn oil true and false method, comprises the steps:
(1) by the δ of glycerine in the corn oil in the known source of mensuration
13c value, builds glycerine δ in true corn oil
13the database of C, glycerine δ in corn oil when drawing fiducial probability 95%
13the distribution range of C value;
(2) δ of measuring samples glycerine is measured
13c value;
(3) by the glycerine δ of measuring samples
13the distribution range of C value and corn oil carries out significant difference analysis;
(4) if there is significant difference, then show that measuring samples is not real corn oil, otherwise be then corn oil.
3. the carbon isotope technique that utilizes according to any one of claim 1-2 differentiates the method for the corn oil true and false, it is characterized in that, described carbon isotope technique is the technology of glycerine carbon isotope composition in gas chromatography-burning-Stable isotope ratio mass spectroscopy grease.
4. the carbon isotope technique that utilizes according to any one of claim 1-3 differentiates the method for the corn oil true and false, it is characterized in that, described gas chromatography-burning-Stable isotope ratio mass-spectrometric technique is specially: flame tube temperature 1000 DEG C, and filler is cupric oxide; Gas chromatographic sample introduction mouth temperature is higher than 200 DEG C, and high-purity helium is as carrier gas, and flow velocity is 1.2mL/min, split ratio 20:1; Quartz capillary chromatographic column, temperature programme is 120 DEG C/1min//20//220 DEG C/7min.
5. the carbon isotope technique that utilizes according to any one of claim 1-4 differentiates the method for the corn oil true and false, it is characterized in that, described oil sample obtains the glycerin component analyzed through pre-service, described preprocess method comprises the steps:
A () is got edible oil sample and is dissolved in isooctane, fully shake;
B () adds potassium hydroxide methanol solution in above-mentioned solution, layering after fully leaving standstill after concussion, removes upper solution, obtain lower floor settled solution b;
C () acidifying b solution, adds isooctane, removes upper solution after solution layering, obtain mixed solution c; With
D the solution obtained in () adjustment c is about 7 to pH, and add absolute methanol solution, obtains glycerine methanol solution d.
6. method according to claim 5, the acidifying wherein in step (c) is undertaken by hydrochloric acid.
7. method according to claim 5, the acidifying wherein in step (c) is undertaken by concentrated hydrochloric acid.
8. method according to claim 5, wherein in step (d), the adjustment of pH is undertaken by supersaturation potassium hydroxide methanol solution.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510822043.2A CN105445404A (en) | 2015-11-24 | 2015-11-24 | Corn oil authentication method adopting stable carbon isotope technique |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510822043.2A CN105445404A (en) | 2015-11-24 | 2015-11-24 | Corn oil authentication method adopting stable carbon isotope technique |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105445404A true CN105445404A (en) | 2016-03-30 |
Family
ID=55555843
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510822043.2A Pending CN105445404A (en) | 2015-11-24 | 2015-11-24 | Corn oil authentication method adopting stable carbon isotope technique |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105445404A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108037213A (en) * | 2017-12-28 | 2018-05-15 | 浙江大学 | A kind of gaschromatographic mass spectrometry method for measuring camellia seed oil and olive oil triglycerides |
| CN111257451A (en) * | 2020-02-06 | 2020-06-09 | 中国食品发酵工业研究院有限公司 | Method for detecting exogenously added synthetic propionic acid or salt thereof in food |
| CN111257452A (en) * | 2020-02-06 | 2020-06-09 | 中国食品发酵工业研究院有限公司 | Method for detecting synthetic acetic acid added in Zhenjiang aromatic vinegar |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103901094A (en) * | 2014-04-22 | 2014-07-02 | 武汉矽感科技有限公司 | Oil detection and identification method based on ion mobility spectrometer |
| US20150196248A1 (en) * | 2014-01-10 | 2015-07-16 | The United States Of America, As Represented By The Secretary Of Agriculture | Methods of determining energy balance using breath carbon isotope ratios |
-
2015
- 2015-11-24 CN CN201510822043.2A patent/CN105445404A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20150196248A1 (en) * | 2014-01-10 | 2015-07-16 | The United States Of America, As Represented By The Secretary Of Agriculture | Methods of determining energy balance using breath carbon isotope ratios |
| CN103901094A (en) * | 2014-04-22 | 2014-07-02 | 武汉矽感科技有限公司 | Oil detection and identification method based on ion mobility spectrometer |
Non-Patent Citations (5)
| Title |
|---|
| ELSA CAYTAN ET AL: "Strategy for specific isotope ratio determination by quantitative NMR on symmetrical molecules: application to glycerol", 《TETRAHEDRON: ASYMMETRY》 * |
| GIOVANNI CALDERONE ET AL: "Characterization of European Wine Glycerol：Stable Carbon Isotope Approach", 《JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY》 * |
| GIOVANNI FRONZA ET AL: "Determination of the 13C Content of Glycerol Samples of Different Origin", 《JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY》 * |
| 中华人民共和国国家质量监督检验检疫总局 中国国家标准化管理委员会: "《中华人民共和国国家标准 GB/T 17376-2008/ISO 5509：2000》", 4 November 2008 * |
| 郭莲仙 等: "基于稳定碳同位素示踪技术的商品植物油掺杂鉴别研究", 《现代食品科技》 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108037213A (en) * | 2017-12-28 | 2018-05-15 | 浙江大学 | A kind of gaschromatographic mass spectrometry method for measuring camellia seed oil and olive oil triglycerides |
| CN111257451A (en) * | 2020-02-06 | 2020-06-09 | 中国食品发酵工业研究院有限公司 | Method for detecting exogenously added synthetic propionic acid or salt thereof in food |
| CN111257452A (en) * | 2020-02-06 | 2020-06-09 | 中国食品发酵工业研究院有限公司 | Method for detecting synthetic acetic acid added in Zhenjiang aromatic vinegar |
| CN111257452B (en) * | 2020-02-06 | 2023-10-27 | 中国食品发酵工业研究院有限公司 | Method for detecting addition of synthetic acetic acid into Zhenjiang aromatic vinegar |
| CN111257451B (en) * | 2020-02-06 | 2023-10-31 | 中国食品发酵工业研究院有限公司 | Method for detecting exogenous addition of synthetic propionic acid or salt thereof in food |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Huang et al. | Improved generalization of spectral models associated with Vis-NIR spectroscopy for determining the moisture content of different tea leaves | |
| CN103454335B (en) | A kind of integrated olfactory analog instrument of hyperchannel and biological fermentation process on-line analysis | |
| CN105259293B (en) | Differentiate the method in the olive oil place of production based on isotope mass spectrometry technology | |
| CN103398970A (en) | Method for qualitatively and quantitatively analyzing edible oil and further detecting hogwash oil | |
| EP3527969A3 (en) | Optical analyzer for identification of materials using transmission spectroscopy | |
| Lukić et al. | Lipid profiling and stable isotopic data analysis for differentiation of extra virgin olive oils based on their origin | |
| Jeon et al. | A triple-isotope approach for discriminating the geographic origin of Asian sesame oils | |
| CN109541060B (en) | Method for identifying adulteration of honey through protein detection | |
| CN105445404A (en) | Corn oil authentication method adopting stable carbon isotope technique | |
| CN106053701B (en) | A kind of liquor classification method | |
| CN103217393A (en) | Detection method for adulterated tea oil | |
| CN104132905A (en) | Detection method for adulterated sesame oil | |
| CN110501439A (en) | A kind of Stable isotope ratio identifies the analysis method of milk different sources | |
| CN104237409A (en) | Construction method for GC-MS (Gas Chromatography-Mass Spectrometer) fingerprint of edible oil and application of GC-MS fingerprint | |
| Li et al. | Multifactor colorimetric analysis on pH-indicator papers: an optimized approach for direct determination of ambient aerosol pH | |
| CN105548064A (en) | Method for determination of multiple nutrient compositions and antibiotic titer changes by using near infrared spectroscopy during production process of antibiotics from microbial fermentation | |
| CN105044025A (en) | Method for fast recognizing sesame oil and sesame oil doped with soybean oil through near infrared | |
| CN104111274A (en) | Method for identifying producing area of red bayberry juice by using gas sensor array type electronic nose fingerprint analysis system | |
| CN106053659A (en) | Method for measuring ratio of nicotine carbon, hydrogen and nitrogen stable isotopes in tobacco | |
| Fu et al. | Authentication of geographical origin in Hainan partridge tea (Mallotus obongifolius) by stable isotope and targeted metabolomics combined with chemometrics | |
| CN113777190A (en) | Method for separating and measuring sanshool content in pepper extract and pepper extract product and application of sanshool content | |
| Xu et al. | Classification of organic and ordinary kiwifruit by chemometrics analysis of elemental fingerprint and stable isotopic ratios | |
| CN102706814A (en) | Rapid melamine determination method using bare gold nanoparticles as developing probe | |
| Xu et al. | Determining the geographical origin of milk by multivariate analysis based on stable isotope ratios, elements and fatty acids | |
| Jin et al. | Developing an authentication approach using SPME-GC-IRMS based on compound-specific δ 13C analysis of six typical volatiles in wine |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20160330 |
|
| RJ01 | Rejection of invention patent application after publication |