CN110132957B - General presentation method for common metal imprinting on surface of human skin - Google Patents
General presentation method for common metal imprinting on surface of human skin Download PDFInfo
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- CN110132957B CN110132957B CN201910527084.7A CN201910527084A CN110132957B CN 110132957 B CN110132957 B CN 110132957B CN 201910527084 A CN201910527084 A CN 201910527084A CN 110132957 B CN110132957 B CN 110132957B
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- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 claims abstract description 44
- 229910052725 zinc Inorganic materials 0.000 claims abstract description 44
- 239000011701 zinc Substances 0.000 claims abstract description 44
- 239000010949 copper Substances 0.000 claims abstract description 25
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 claims abstract description 24
- 229910052802 copper Inorganic materials 0.000 claims abstract description 24
- 229910052782 aluminium Inorganic materials 0.000 claims abstract description 23
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 claims abstract description 23
- 229910052742 iron Inorganic materials 0.000 claims abstract description 22
- 239000011133 lead Substances 0.000 claims abstract description 21
- 238000005507 spraying Methods 0.000 claims abstract description 18
- 238000012795 verification Methods 0.000 claims abstract description 17
- 239000003086 colorant Substances 0.000 claims abstract description 13
- 239000000243 solution Substances 0.000 claims description 39
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 34
- KMVWNDHKTPHDMT-UHFFFAOYSA-N 2,4,6-tripyridin-2-yl-1,3,5-triazine Chemical compound N1=CC=CC=C1C1=NC(C=2N=CC=CC=2)=NC(C=2N=CC=CC=2)=N1 KMVWNDHKTPHDMT-UHFFFAOYSA-N 0.000 claims description 23
- OAEGRYMCJYIXQT-UHFFFAOYSA-N dithiooxamide Chemical compound NC(=S)C(N)=S OAEGRYMCJYIXQT-UHFFFAOYSA-N 0.000 claims description 22
- HNVCXDAVEHOIBP-UHFFFAOYSA-N 2-[(5-bromopyridin-2-yl)diazenyl]-5-(diethylamino)phenol Chemical compound OC1=CC(N(CC)CC)=CC=C1N=NC1=CC=C(Br)C=N1 HNVCXDAVEHOIBP-UHFFFAOYSA-N 0.000 claims description 20
- SXWPCWBFJXDMAI-UHFFFAOYSA-L disodium;3,4,5,6-tetraoxocyclohexene-1,2-diolate Chemical compound [Na+].[Na+].[O-]C1=C([O-])C(=O)C(=O)C(=O)C1=O SXWPCWBFJXDMAI-UHFFFAOYSA-L 0.000 claims description 17
- 239000012916 chromogenic reagent Substances 0.000 claims description 16
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- 108010007337 Azurin Proteins 0.000 claims description 13
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- 125000004105 2-pyridyl group Chemical group N1=C([*])C([H])=C([H])C([H])=C1[H] 0.000 description 1
- OTMYLOBWDNFTLO-UHFFFAOYSA-N 5,6-diphenyl-3-(2-pyridyl)-1,2,4-triazine Chemical compound C1=CC=CC=C1C1=NN=C(C=2N=CC=CC=2)N=C1C1=CC=CC=C1 OTMYLOBWDNFTLO-UHFFFAOYSA-N 0.000 description 1
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Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/29—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands using visual detection
- G01N21/293—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands using visual detection with colour charts, graduated scales or turrets
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N2021/775—Indicator and selective membrane
Abstract
The invention relates to a general developing method of common metal print on the surface of human skin, which uses a comparison color card containing color blocks with different colors, and comprises the following steps: preparing general revealing reagent and verifying revealing reagent of copper, iron, lead, aluminum and zinc print respectively; spraying a universal color reagent on the skin surface of the person to be detected, which is possibly contacted with the metal tool, at room temperature, finding out a comparison color card where a first color block with the same color as the metal print after universal appearance is located, and preliminarily judging the metal print type corresponding to the comparison color card; spraying verification and display reagent corresponding to the metal print types to the same part, comparing the color of the metal print after verification and display with the color of the second color block of the selected comparison color card, and confirming the metal print types if the colors are consistent. The method can universally show the residual marks of iron, zinc, copper, lead and aluminum tools on the surface of human skin, and provides a unified showing sequence and a discrimination standard for the five showing methods.
Description
Technical Field
The invention belongs to the technical field of court scientific trace identification, and particularly relates to a general presentation method of a common metal trace of human skin.
Background
The metal print showing technology is a field quick showing method for showing trace metal transferred to the skin surface in the contact process of a perpetrator and a metal tool, and reflecting the outline and detail characteristics of the metal tool, so as to prove the relevance between the perpetrator and the metal tool. The method not only can provide investigation clues for criminal cases and terrorism events involving metal tools, but also can be used as mutual evidence of associated material evidence and audiovisual evidence.
Until now, iron tools such as guns, cutters and the like have been the main research object in the field at home and abroad. The use of 3- (2-pyridyl) -5, 6-diphenyl-1, 2, 4-triazine (abbreviated as "PDT") or 2,4, 6-tris (2-pyridyl) -1,3, 5-triazine (abbreviated as "TPTZ") reagents, respectively, successfully revealed the carryover of ferrous tools on the surface of human skin. In addition, the zinc plating tool legacy print and the iron ware legacy print thereof are subjected to universality development (ZL 201510018897.5) by adopting a 2- (5-bromo-2-pyridylazo) -5- (diethylamino) phenol (abbreviated as '5-Br-PADAP') reagent. However, in addition to the two metal blots described above, non-ferrous metal tools such as copper, aluminum, and alloy tools have a high frequency of use in criminal cases and terrorism events as well. If the potential metallic print left by these tools cannot be quickly visualized in the field, important case cues may be missed.
In addition, the investigation personnel often cannot predict the type of metal tool that will be a crime prior to inspection. Although various metal print visualization methods are established, the use sequence, interaction, result judgment and the like are all problems to be solved. Moreover, the currently established metal print visualization method focuses on obtaining morphological characteristics of metal tools, and often neglects to mine print component information. If more accurate compositional information can be provided by blotting visualization, it will play a greater role in inferring suspected metal tool types.
Disclosure of Invention
The invention aims to solve the technical problem that in the prior art, a chromogenic reagent only can show the residual marks of an iron tool and a galvanized tool on the surface of human skin.
Another technical problem to be solved by the present invention is the lack of a uniform development sequence between the various metal print development methods.
The invention aims to solve the other technical problem that a method for distinguishing the color of the metal print after the surface of the human skin appears is lacked.
In order to solve the technical problems, the invention provides a general appearance method of common metal marks on the surface of human skin, which is characterized in that the method uses the following metal marks to compare color cards: copper print comparison color card, iron print comparison color card, lead print comparison color card, zinc print comparison color card and aluminum print comparison color card; each metal print comparison color card respectively comprises a main body with an observation hole in the middle, a first color block and a second color block with different colors are respectively fixed on the main body positioned at two sides of the observation hole, the colors of the metal print to be compared which are developed twice in sequence are respectively represented, and corresponding metal print type identifiers are respectively arranged on the color blocks;
the general presentation method comprises the following steps:
1) Respectively weighing six kinds of color reagent powder of 2- (5-bromo-2-pyridine azo) -5-diethylaminophenol, dithiooxamide, 2,4, 6-tri (2-pyridyl) -1,3, 5-triazine, sodium rhodizonate, chrome azurin S and zinc reagent; respectively dissolving the weighed 2- (5-bromo-2-pyridylazo) -5-diethylaminophenol, dithiooxamide and 2,4, 6-tris (2-pyridyl) -1,3, 5-triazine in absolute ethyl alcohol, dissolving the weighed sodium rhodizonate in deionized water, respectively dissolving the weighed chromium azurin S and zinc reagent in an ethanol solution to respectively obtain a chromogenic reagent solution with the mass concentration of 0.1-5 g/L; taking a 2- (5-bromo-2-pyridylazo) -5-diethylaminophenol chromogenic reagent solution as a general revealing reagent, and taking dithiooxamide, 2,4, 6-tris (2-pyridyl) -1,3, 5-triazine, sodium rhodizonate, chromium azurin S and a zinc chromogenic reagent solution as verifying revealing reagents of copper, iron, lead, aluminum and zinc blots respectively;
2) Uniformly spraying the general developing reagent on the part, possibly contacting with a metal tool, of the skin surface of a person to be detected at room temperature, and taking a metal print as a first sample after the metal print is completely developed;
3) The color of a first color block on the metal print comparison color card is compared with the color of the metal print in the first sample through the observation holes of the metal print comparison color cards, and if the color of the metal print in the first sample is the same as the color of the first color block of a certain metal print comparison color card, the metal tool type is primarily judged to be the metal type represented by the metal print comparison color card;
4) Uniformly spraying verification developing reagent corresponding to the metal species preliminarily determined in the step 3) on the skin surface part of the person to be detected sprayed with the universal developing reagent in the step 2), and photographing the metal imprinting after complete color development to obtain a second sample;
5) And 3) comparing the color of the second color block with the color of the metal print in the second sample through the observation hole of the metal print comparison color card selected in the step 3), and if the two colors are the same, confirming that the metal tool type is the metal type represented by the metal print comparison color card.
Further, the first color block and the second color block of the copper print comparison color card are respectively pink and dark green, and the first color block and the second color block of the iron print comparison color card are respectively purple and purple; the first color block and the second color block of the zinc print comparison color card are respectively rose color and bluish purple, the first color block and the second color block of the lead print comparison color card are respectively light pink and reddish brown, and the first color block and the second color block of the aluminum print comparison color card are respectively yellow and deep purple.
Further, in the step 1), ethanol with the volume fraction of 25-75% is adopted to dissolve the chrome azurin S and the zinc reagent.
Further, in the step 2), the amount of the sprayed universal developing reagent is 0.1 to 1.0mL.
Further, in the step 4), the sprayed verification visualization reagent is used in an amount of 0.1 to 1.0mL.
Compared with the prior art, the invention has the beneficial effects that:
1) Multiple metal print display methods are established, the universality display of common metal print on the surface of human skin is realized, and the application range of the prior art is expanded.
2) On the basis of guaranteeing the integrity of morphological characteristics of the metal imprinting, the method can rapidly and accurately verify various element information in the imprinting, improve the reliability and accuracy of the original manifestation method, and enhance the evidence effectiveness of the manifestation imprinting.
3) The method for distinguishing the metal print types is established, and the investigation personnel can distinguish the metal print types on site by comparing the developed metal print colors with the color of the comparison color card.
4) Six metal print displaying methods on the surface of human skin are integrated, and a unified operation flow and a clear distinguishing method are provided for the metal print displaying method.
Drawings
Other features and advantages of the present invention will become more apparent from the following detailed description of embodiments of the present invention, which is to be read in connection with the accompanying drawings.
FIG. 1 is a schematic diagram of the structure of each metal print comparison color card used in the method of the invention.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention.
The invention provides a general metal print showing method for the surface of human skin, which is characterized in that a metal print comparison color card shown in figure 1 is needed to be utilized, and the comparison color card consists of a copper (Cu) print comparison color card, an iron (Fe) print comparison color card, a lead (Pb) print comparison color card, a zinc (Zn) print comparison color card and an aluminum (Al) print comparison color card. Each metal print comparison color card respectively comprises a main body 1 with an observation hole 2 in the middle, a first color block 3 and a second color block 4 with different colors are respectively fixed on the main body 1 positioned at two sides of the observation hole 2, the colors of the metal print to be compared which are developed twice in sequence are respectively represented, and corresponding metal print type identifiers 5 and presentation sequence identifiers 6 are respectively arranged on the color blocks (the presentation sequence identifiers refer to a general presentation and a verification presentation shown in the figure 1); the first color block and the second color block of the copper print comparison color card are respectively pink and dark green (it is to be noted that, as a color drawing cannot be provided, the drawing is subjected to gray scale processing, and the corresponding colors of the color blocks in the drawing are distinguished by the depths of the color blocks, and the following is the same), and the first color block and the second color block of the copper print comparison color card are respectively purple and purple; the first color block and the second color block of the zinc print comparison color card are respectively rose color and bluish purple, the first color block and the second color block of the lead print comparison color card are respectively light pink and reddish brown, and the first color block and the second color block of the aluminum print comparison color card are respectively yellow and deep purple.
Further, the main body 1 of each metal imprinting comparison color card is rectangular with the same size, the length is 5-7 cm, the width is 3-5 cm, and four corners of the main body are rounded corners. The main body 1 can adopt coated paper, white cardboard, color laser paper, printing photographic paper or copying paper. The middle part of the main body 1 is provided with a circular observation hole 2, the diameter of the observation hole is 70-80% of the width of the main body 1, different color-developing reagents are sprayed on the skin surface of a person to be tested with a metal print, after the skin is completely developed, the skin color is observed through the observation hole 2, the color is sequentially compared with the color blocks on two sides of the observation hole (the color blocks are compared with a first color block after the first spraying is completely developed, the color blocks are compared with a second color block after the second spraying is completely developed), and if the colors are completely matched, the type of the metal print on the skin surface of the person to be tested can be judged.
Further, the surface plastic package of each metal print comparison color card is provided with a transparent protective film, so that pollution to the color card caused by print after appearance is prevented, and the durability of the color card is improved.
The following are examples of the present invention for general visualization of common metal blots on the skin surface of a human body using the above metal blot comparison color card:
example 1, a general metal blot visualization method was used to visualize copper bar carryover blots on the skin of hand, comprising the specific steps of:
1) 20mg of each of six color-developer powders of 2- (5-bromo-2-pyridylazo) -5-diethylaminophenol, dithiooxamide, 2,4, 6-tris (2-pyridyl) -1,3, 5-triazine, sodium rhodiola-acid, chromsonil S and zinc reagent were weighed out. Subsequently, the weighed 2- (5-bromo-2-pyridylazo) -5-diethylaminophenol, dithiooxamide and 2,4, 6-tris (2-pyridyl) -1,3, 5-triazine are respectively dissolved in 10mL of absolute ethyl alcohol, the weighed sodium rhodizonate is dissolved in 10mL of deionized water, the weighed chromium azurin S and zinc reagent are respectively dissolved in ethanol solution with the volume fraction of 50% (V: V=1:1), and six color reagent solutions with the concentration of 2g/L are respectively prepared and are respectively placed in a spray can for storage; 2- (5-bromo-2-pyridinazo) -5-diethylaminophenol chromogenic reagent solution was used as a general visualization reagent, and dithiooxamide, 2,4, 6-tris (2-pyridyl) -1,3, 5-triazine, sodium rhodizonate, chromene S and zinc chromogenic reagent solutions were used as verification visualization reagents for copper, iron, lead, aluminum and zinc blots, respectively.
2) And uniformly spraying 0.3mL of a general reagent 2- (5-bromo-2-pyridylazo) -5-diethylaminophenol solution on the skin surface of the person to be detected, which is possibly contacted with the metal tool, and taking a metal print as a first sample after complete color development (for example, after 2min interval).
3) And comparing the color of the first color block with the color of the metal print in the first sample through the observation hole of each metal print comparison color card. After comparison, the color of the metal print after general appearance is pink and purple, and is consistent with the color of the first color block of the copper print comparison color card, and the copper tool left print is preliminarily judged.
4) Uniformly spraying verification revealing reagent, namely 0.5mL dithiooxamide solution, corresponding to the metal species preliminarily judged in the step 3) on the surface part (namely the original metal imprinting part) of the human skin to be detected, sprayed with the universal revealing reagent in the step 2), and photographing the metal imprinting after complete color development (for example, after 2 minutes interval) to obtain a second sample.
5) And 3) comparing the color of the second color block with the color of the metal print in the second sample through the observation hole of the metal print comparison color card (copper print comparison color card in the embodiment) selected in the step 3). And after comparison, verifying that the color of the print after appearance is dark green, conforming to the color of a second color block on the copper print comparison color card, and confirming that the print is left behind by the copper tool.
Example 2: the method for displaying the deformed steel bar left-behind marks on the hand skin by adopting a metal mark general display method specifically comprises the following steps:
1) 30mg of each of six color-developer powders of 2- (5-bromo-2-pyridylazo) -5-diethylaminophenol, dithiooxamide, 2,4, 6-tris (2-pyridyl) -1,3, 5-triazine, sodium rhodiola-acid, chromsonil S and zinc reagent were weighed out. Subsequently, the weighed 2- (5-bromo-2-pyridylazo) -5-diethylaminophenol, dithiooxamide and 2,4, 6-tris (2-pyridyl) -1,3, 5-triazine were dissolved in 10mL of absolute ethanol, the weighed sodium rhodizonate was dissolved in 10mL of deionized water, and the weighed chromium azurin S and zinc reagent were dissolved in 50% ethanol solution (V: V=1:1) by volume fraction, respectively, to prepare 3 g.L concentrations -1 Respectively placing the six color reagent solutions in a spray can for storage; 2- (5-bromo-2-pyridinazo) -5-diethylaminophenol chromogenic reagent solution was used as a general visualization reagent, and dithiooxamide, 2,4, 6-tris (2-pyridyl) -1,3, 5-triazine, sodium rhodizonate, chromene S and zinc chromogenic reagent solutions were used as verification visualization reagents for copper, iron, lead, aluminum and zinc blots, respectively.
2) And uniformly spraying 0.2mL of a general reagent 2- (5-bromo-2-pyridylazo) -5-diethylaminophenol solution on the skin surface of the person to be detected, which is possibly contacted with the metal tool, and taking a metal print as a first sample after complete color development (for example, after 2min interval).
3) And comparing the color of the first color block with the color of the metal print in the first sample through the observation hole of each metal print comparison color card. After comparison, the color of the metal print after universal appearance is mauve, is consistent with the color of the first color block of the iron print comparison color card, and is preliminarily judged to be the iron tool left print.
4) Uniformly spraying 0.3mL of verification revealing reagent corresponding to the metal species preliminarily judged in the step 3, namely 2,4, 6-tris (2-pyridyl) -1,3, 5-triazine solution, on the surface part (namely the original metal imprinting part) of the human skin to be detected, sprayed with the universal revealing reagent in the step 2), and photographing the metal imprinting after complete color development (for example after interval of 2 minutes) to obtain a second sample.
5) And 3) comparing the color of the second color block with the color of the metal print in the second sample through the observation hole of the metal print comparison color card (the iron print comparison color card in the embodiment) selected in the step 3). And after comparison, verifying that the developed print is purple, conforming to the color of the second color block of the iron print comparison color card, and confirming that the iron tool is left behind.
Example 3: the method for displaying the zinc-plated pipe left-behind marks on the skin of the hand by adopting a metal mark general display method comprises the following steps:
1) 10mg of each of the six color developer powders of 2- (5-bromo-2-pyridylazo) -5-diethylaminophenol, dithiooxamide, 2,4, 6-tris (2-pyridyl) -1,3, 5-triazine, sodium rhodiola-acid, chromsonil S and zinc reagent were weighed out. Subsequently, the weighed 2- (5-bromo-2-pyridylazo) -5-diethylaminophenol, dithiooxamide and 2,4, 6-tris (2-pyridyl) -1,3, 5-triazine were dissolved in 10mL of absolute ethanol, the weighed sodium rhodizonate was dissolved in 10mL of deionized water, and the weighed chromium azurin S and zinc reagent were dissolved in 50% ethanol solution (V: V=1:1) by volume fraction, respectively, to prepare 1 g.L concentrations -1 Respectively placing the six color reagent solutions in a spray can for storage; developing the color of 2- (5-bromo-2-pyridylazo) -5-diethylaminophenolThe reagent solution is used as a general visualization reagent, and dithiooxamide, 2,4, 6-tri (2-pyridyl) -1,3, 5-triazine, sodium rhodizonate, chrome azure S and zinc chromogenic reagent solutions are respectively used as verification visualization reagents of copper, iron, lead, aluminum and zinc blots.
2) And uniformly spraying 0.5mL of a general reagent 2- (5-bromo-2-pyridylazo) -5-diethylaminophenol solution on the skin surface of the person to be detected, which is possibly contacted with the metal tool, and taking a picture of the metal print as a first sample after complete color development (for example, after 2min interval).
3) And comparing the color of the first color block with the color of the metal print in the first sample through the observation hole of each metal print comparison color card. After comparison, the color of the metal print after general appearance is in a rose pink color, which is consistent with the color of the first color block of the zinc print comparison color card, and the metal print is primarily judged to be the zinc (or galvanized) tool left print.
4) Uniformly spraying verification revealing reagent, namely zinc reagent solution, 0.5mL corresponding to the metal species preliminarily judged in the step 3) on the surface part (namely the original metal imprinting part) of the skin to be detected, sprayed with the universal revealing reagent in the step 2), and photographing the metal imprinting after complete color development (for example after 2 minutes interval) to obtain a second sample.
5) And 3) comparing the color of the second color block with the color of the metal print in the second sample through the observation hole of the metal print comparison color card (zinc print comparison color card in the embodiment) selected in the step 3). And after comparison, verifying that the developed print is bluish purple, and conforming to the color of the second color block of the zinc print comparison color card, and confirming that the print is left behind by a zinc (or galvanized) tool.
Example 4: the method for displaying the lead wire left-behind marks on the skin of the hand by adopting a metal mark universal display method comprises the following steps:
1) 2mg of 2- (5-bromo-2-pyridylazo) -5-diethylaminophenol, dithiooxamide, 2,4, 6-tris (2-pyridyl) -1,3, 5-triazine, sodium rhodiola-ate, chromsonil S and zinc reagent were weighed out respectively as six developer powders. Subsequently, the weighed 2- (5-bromo-2-pyridylazo) -5-diethylaminophenol, dithiooxamide and 2,4, 6-tris (2-pyridyl) are combined) -1,3, 5-triazine is respectively dissolved in 10mL of absolute ethyl alcohol, the weighed sodium rhodizonate is dissolved in 10mL of deionized water, the weighed chrome azurin S and zinc reagent are respectively dissolved in ethanol solution with the volume fraction of 50% (V: V=1:1) to be respectively prepared into the concentration of 2 g.L -1 Respectively placing the six color reagent solutions in a spray can for storage; 2- (5-bromo-2-pyridinazo) -5-diethylaminophenol chromogenic reagent solution was used as a general visualization reagent, and dithiooxamide, 2,4, 6-tris (2-pyridyl) -1,3, 5-triazine, sodium rhodizonate, chromene S and zinc chromogenic reagent solutions were used as verification visualization reagents for copper, iron, lead, aluminum and zinc blots, respectively.
2) And uniformly spraying 0.1mL of a general reagent 2- (5-bromo-2-pyridylazo) -5-diethylaminophenol solution on the skin surface of the person to be detected, which is possibly contacted with the metal tool, and taking a metal print as a first sample after complete color development (for example, after 2min interval).
3) And comparing the color of the first color block with the color of the metal print in the first sample through the observation hole of each metal print comparison color card. After comparison, the color of the metal print after general appearance is light pink, and is consistent with the color of the first color block of the lead print comparison color card, and the lead print is primarily judged to be left behind by the lead tool.
4) Uniformly spraying verification revealing reagent corresponding to the metal species preliminarily judged in the step 3) on the surface part (namely the original metal imprinting part) of the human skin to be detected sprayed with the universal revealing reagent in the step 2), namely 0.5mL of sodium rhodizonate solution, and photographing the metal imprinting after complete color development (for example after 2 minutes interval) to obtain a second sample.
5) And 3) comparing the color of the second color block with the color of the metal print in the second sample through the observation hole of the metal print comparison color card (lead print comparison color card in the embodiment) selected in the step 3). And (3) comparing, verifying that the developed print is reddish brown, and conforming to the color of the second color block of the lead print comparison color card, and confirming that the print is left by a lead tool.
Example 5: the universal metal print showing method is adopted to show the aluminum bar left print on the hand skin, and specifically comprises the following steps:
1) 1mg of 2- (5-bromo-2-pyridylazo) -5-diethylaminophenol, dithiooxamide, 2,4, 6-tris (2-pyridyl) -1,3, 5-triazine, sodium rhodiola-ate, chromsonil S and zinc reagent were weighed out respectively as six developer powders. Subsequently, the weighed 2- (5-bromo-2-pyridylazo) -5-diethylaminophenol, dithiooxamide and 2,4, 6-tris (2-pyridyl) -1,3, 5-triazine were dissolved in 10mL of absolute ethanol, the weighed sodium rhodizonate was dissolved in 10mL of deionized water, and the weighed chromium azurin S and zinc reagent were dissolved in 50% ethanol solution (V: V=1:1) by volume fraction, respectively, to prepare 1 g.L concentrations -1 Respectively placing the six color reagent solutions in a spray can for storage; 2- (5-bromo-2-pyridinazo) -5-diethylaminophenol chromogenic reagent solution was used as a general visualization reagent, and dithiooxamide, 2,4, 6-tris (2-pyridyl) -1,3, 5-triazine, sodium rhodizonate, chromene S and zinc chromogenic reagent solutions were used as verification visualization reagents for copper, iron, lead, aluminum and zinc blots, respectively.
2) And uniformly spraying 0.3mL of a general reagent 2- (5-bromo-2-pyridylazo) -5-diethylaminophenol solution on the skin surface of the person to be detected, which is possibly contacted with the metal tool, and taking a metal print as a first sample after complete color development (for example, after 2min interval).
3) And comparing the color of the first color block with the color of the metal print in the first sample through the observation hole of each metal print comparison color card. After comparison, the color of the metal print after universal appearance is yellow, and is consistent with the color of the first color block on the aluminum print comparison color card, and the metal print is preliminarily judged to be the residual print of the aluminum tool.
4) Uniformly spraying verification revealing reagent corresponding to the metal species preliminarily judged in the step 3) to the surface part (namely the original metal imprinting part) of the skin to be detected, sprayed with the universal revealing reagent in the step 2), namely 0.6mL of chrome azure S solution, and photographing the metal imprinting after complete color development (for example after 2 minutes interval) to obtain a second sample.
5) And 3) comparing the color of the second color block with the color of the metal print in the second sample through the observation hole of the metal print comparison color card (in the embodiment, the aluminum print comparison color card) selected in the step 3). And after comparison, verifying that the developed print is dark purple, and conforming to the color of a second color block on the aluminum print comparison color card, and confirming that the print is left behind by the aluminum tool.
In conclusion, the method can be used for displaying iron tools, galvanization tools, copper tools, lead tools and aluminum tools on the surface of human skin universally, providing unified display sequence and discrimination standard for the five display methods, rapidly and accurately verifying various element information in the print on the basis of guaranteeing the morphological feature integrity of the metal print, improving the reliability and accuracy of the original display method and enhancing the evidence effectiveness of the display print.
The above embodiment is a preferred embodiment of the present invention, but the embodiment of the color chart is not limited by the above embodiment, and any other changes, modifications, substitutions, combinations, and simplifications made without departing from the spirit and principles of the present invention should be equivalent substitution manner, and are included in the protection scope of the present invention.
Claims (9)
1. The universal visualization method for the common metal print on the surface of the human skin is characterized by comprising the following steps of: copper print comparison color card, iron print comparison color card, lead print comparison color card, zinc print comparison color card and aluminum print comparison color card; each metal print comparison color card respectively comprises a main body with an observation hole in the middle, a first color block and a second color block with different colors are respectively fixed on the main body positioned at two sides of the observation hole, the colors of the metal print to be compared which are developed twice in sequence are respectively represented, and corresponding metal print type identifiers are respectively arranged on the color blocks;
the general presentation method comprises the following steps:
1) Respectively weighing six kinds of color reagent powder of 2- (5-bromo-2-pyridine azo) -5-diethylaminophenol, dithiooxamide, 2,4, 6-tri (2-pyridyl) -1,3, 5-triazine, sodium rhodizonate, chrome azurin S and zinc reagent; respectively dissolving the weighed 2- (5-bromo-2-pyridylazo) -5-diethylaminophenol, dithiooxamide and 2,4, 6-tris (2-pyridyl) -1,3, 5-triazine in absolute ethyl alcohol, dissolving the weighed sodium rhodizonate in deionized water, respectively dissolving the weighed chromium azurin S and zinc reagent in an ethanol solution to respectively obtain a chromogenic reagent solution with the mass concentration of 0.1-5 g/L; taking a 2- (5-bromo-2-pyridylazo) -5-diethylaminophenol chromogenic reagent solution as a general revealing reagent, and taking dithiooxamide, 2,4, 6-tris (2-pyridyl) -1,3, 5-triazine, sodium rhodizonate, chromium azurin S and a zinc chromogenic reagent solution as verifying revealing reagents of copper, iron, lead, aluminum and zinc blots respectively;
2) Uniformly spraying the general developing reagent on the part, possibly contacting with a metal tool, of the skin surface of a person to be detected at room temperature, and taking a metal print as a first sample after the metal print is completely developed;
3) The color of a first color block on the metal print comparison color card is compared with the color of the metal print in the first sample through the observation holes of the metal print comparison color cards, and if the color of the metal print in the first sample is the same as the color of the first color block of a certain metal print comparison color card, the metal tool type is primarily judged to be the metal type represented by the metal print comparison color card;
4) Uniformly spraying verification developing reagent corresponding to the metal species preliminarily determined in the step 3) on the skin surface part of the person to be detected sprayed with the universal developing reagent in the step 2), and photographing the metal imprinting after complete color development to obtain a second sample;
5) And 3) comparing the color of the second color block with the color of the metal print in the second sample through the observation hole of the metal print comparison color card selected in the step 3), and if the two colors are the same, confirming that the metal tool type is the metal type represented by the metal print comparison color card.
2. The method according to claim 1, wherein in the step 1), ethanol with a volume fraction of 25-75% is used to dissolve the chrome azurin S and the zinc reagent.
3. The universal developing method according to claim 1, wherein the amount of the universal developing reagent sprayed in the step 2) is 0.1 to 1.0mL.
4. The universal presentation method as claimed in claim 1, wherein the sprayed verification presentation reagent is used in an amount of 0.1 to 1.0mL in the step 4).
5. The universal visualization method of claim 1, wherein the first color patch and the second color patch of the copper print comparison color card are respectively pink and dark green, and the first color patch and the second color patch of the iron print comparison color card are respectively purple and purple; the first color block and the second color block of the zinc print comparison color card are respectively rose color and bluish purple, the first color block and the second color block of the lead print comparison color card are respectively light pink and reddish brown, and the first color block and the second color block of the aluminum print comparison color card are respectively yellow and deep purple.
6. The universal presentation method according to claim 1, wherein each of the metal print comparison color cards has a rectangular shape, a length of 5-7 cm and a width of 3-5 cm, and four corners of each of the metal print comparison color cards are rounded.
7. The universal visualization method of claim 1, wherein the viewing aperture is circular and has a diameter of 70-80% of the width of the body.
8. The universal presentation method as claimed in claim 1, wherein the metal print comparison color card is coated paper, white cardboard, color paper, printing photographic paper or copying paper.
9. The general presentation method according to any one of claims 1 to 8, wherein: the surface of each metal print comparison color card is plastic-sealed with a transparent protective film.
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| CN112304933B (en) * | 2020-11-03 | 2021-09-21 | 南通市第一人民医院 | Intelligent disinfectant colorimetric display control method and system |
| CN113030073B (en) * | 2021-02-26 | 2022-11-01 | 公安部物证鉴定中心 | Method for extracting, showing and verifying copper and lead residues in bullet traces |
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