CN110129385A - A method of it improving bacterial strain and produces sour efficiency - Google Patents
A method of it improving bacterial strain and produces sour efficiency Download PDFInfo
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- CN110129385A CN110129385A CN201910201189.3A CN201910201189A CN110129385A CN 110129385 A CN110129385 A CN 110129385A CN 201910201189 A CN201910201189 A CN 201910201189A CN 110129385 A CN110129385 A CN 110129385A
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Abstract
The invention belongs to glutamic acid production technical fields, disclose a kind of method that raising bacterial strain produces sour efficiency, and film coupling dialysis batch fermentation is used to improve fermentation efficiency by adding inositol and magnesium carbonate in ferment middle.
Description
Technical field
The invention belongs to glutamic acid production technical fields, are related to a kind of method that raising bacterial strain produces sour efficiency.
Background technique
Glutamic acid is a kind of acidic amino acid.Molecule includes two carboxyls, and chemical name is alpha-amido glutaric acid.Paddy ammonia
Acid is to find for inner Suo Xun 1856, is clear crystal, has delicate flavour, be slightly soluble in water, and is dissolved in hydrochloric acid solution, isoelectric point 3.22.
Largely it is present in grain protein, content is also more in animal brain.During the protein metabolism of glutamic acid in vivo
Critical role is accounted for, many important chemical reactions in animal, plant and microorganism are participated in.Sodium glutamate is commonly called as monosodium glutamate, is important
Tasty agents, to fragrance have humidification.Sodium glutamate is widely used in food flavor, not only can be used alone, but can and its
Its amino acid etc. is used in combination.For in food, there is flavouring effect.Concentration is 0.2%-0.5% in food, allows to take in for each person every day
Amount is micro- g kg of 0-120 (in terms of glutamic acid).General dosage is 0.2-1.5 gs/kg in food processing.
Corynebacterium glutamicum is the bacterial strain of glutamic acid fermentation, belongs to facultative aerobe, medium component and condition of culture are not
Together, product is also different, and glutamic acid fermentation condition optimizing is mainly two aspect of nutrient media components and Fermentation Process of Parameter control optimization.
During the fermentation, the fermentation character of strain itself is it some times happens that variation, causes batch indirect fermentation performance greatest differences occur.
Once strain fermentation characteristic changes, thallus will decline the adaptability and acid producing ability of environmental change, show as mending
Occurs the phenomenon that " only consume sugar, do not produce acid " after material, final aminoglutaric acid concentration is very low, causes fermenting property unstable.Glutamic acid
The early period of fermentation, main bacterial strain proliferation was rapid, and it is synthesis point that middle and later periods bacterial strain growth rate, which slows down, but glutamic acid synthesis is accelerated
Secrete the critical period of glutamic acid, that is, convert from accumulation type to acid type bacterial strain is produced.
It includes as follows to patented technology " a method of improve glutamic acid fermentation middle and later periods conversion ratio " before applicant
Step: inositol and dimethylformamide are added during the fermentation, and uses ultrasonication, passes through addition suitable two
Methylformamide is conducive to substrate molecule and is easier to enter cell connect with biological enzyme so that the permeability of cell membrane of thallus enhances
Touching, and then improve conversion ratio；Suitable inositol can strengthen the fixed reaction of CO2, promote the accumulation of glutamic acid, improve fermentation and turn
Rate.On the basis of the research, Fu Feng group continues to study fermentation and acid efficiency, it is intended to further promote fermentation
Efficiency.
Summary of the invention
In order to overcome the production acid efficiency of prior art bacterial strain further to be promoted, the invention proposes a kind of raising bacterium
The method that strain produces sour efficiency.
The present invention is achieved by the following technical solution:
A method of it improving bacterial strain and produces sour efficiency, use film coupling dialysis batch fermentation.
Further, the film coupling dialysis batch fermentation is two Batch fermentations.
Further, described method includes following steps:
By Corynebacterium glutamicum seed liquor with the inoculation access of 6-10% inoculum concentration equipped in the 30L fermentor of 21L fermentation medium
Fermented and cultured is carried out, it is 33 DEG C of fermentation temperature, ventilatory capacity 0.4vvm, speed of agitator 500r/min, fermentor and ceramic membrane is even
Connection adds the inositol of 1-2g/L and the magnesium carbonate of 0.5-1g/L when fermentation is to 12h, continues the 12h that ferments, by the hair in fermentor
Zymotic fluid is pumped out via centrifugal pump, then through ceramic membrane separation, obtains filtrate and concentration thallus, filtrate is drained into feed liquid storage tank,
Concentration thallus is returned fermentor, while adding fermentation tank culture medium into fermentor, continues the 12h that ferments, then add 1-2g/L
Inositol and 0.5-1g/L magnesium carbonate, continue ferment 12h, complete fermentation, obtain fermentation liquid, by fermentor fermentation liquid pass through
It is pumped out by centrifugal pump, then through ceramic membrane separation, obtains filtrate and concentration thallus, filtrate is drained into feed liquid storage tank；Entire hair
During ferment, by stream plus GPE defoaming, while the pH value of Feeding ammonia water control fermentation liquid is to 5.5-6.0.
Preferably,
The group of the fermentation tank culture medium is divided into (mass percent): glucose 12%, corn pulp 3%, urea 0.5%, biphosphate
Potassium 0.5%, ferrous sulfate heptahydrate 0.02%, manganese sulfate monohydrate 0.01%, fulvic acid 0.002%.
Preferably,
The molecular cut off of the ceramic membrane is 10000-20000Da.
Preferably,
The preparation process of the Corynebacterium glutamicum seed liquor are as follows:
Corynebacterium glutamicum is inoculated into seed culture medium, at 33 DEG C, 100rpm shaking table culture 12h, obtained glutamic acid rod
Bacillus seed liquor.
Preferably,
The group of the Corynebacterium glutamicum seed culture medium is divided into (being below mass percent): glucose 6%, corn pulp
3%, potassium dihydrogen phosphate 0.2%, epsom salt 0.02%, manganese sulfate monohydrate 0.01%, 115 DEG C of sterilizing 15min；
Compared with prior art, the beneficial effect that the present invention obtains mainly includes but is not limited to the following aspects:
The present invention is used using film coupling dialysis batch fermentation, reduces the production of the substances such as by-product acetic acid, lactic acid in fermentation process
It is raw, fermentation efficiency is improved, and amino acid zymotic fluid transparency is high, impurity is few, and separation and Extraction is more simple, and the later period is easier
Obtain the product of purity is high.
Containing groups such as a large amount of phenolic hydroxyl groups, carbonyls in fulvic acid, electrolysis degree is higher, can promote glutamic acid synthesis process
It is middle to utilize O2As hydrogen acceptor, and then pyruvic acid is reduced as hydrogen acceptor, add suitable fulvic acid in fermentation medium, it can
The production quantity of by-product lactic acid and acetic acid is reduced, and then improves the yield of glutamic acid；
Inositol can strengthen the fixed reaction of CO2, weaken glyoxalic acid circulation, guarantee that tricarboxylic acid cycle is not disrupted and in a steady stream not
Cut-off largely accumulates glutamic acid by reduction of amination to α-ketoglutaric acid, improves fermentation conversion rate；
Magnesium carbonate adds the time as ferment middle, and cell enters secretion by accumulation type and produces acid type, and magnesium carbonate can be with by-product cream
Acid, acetic acidreaction decompose and generate carbon dioxide, can provide carbon dioxide, are in CO2 content and both maintain thallus eupnea
Effect also ensures that more CO2 are fixed and generates oxaloacetic acid and acetyl-CoA synthesizing citric acid, and it is required to provide synthesis glutamic acid institute
C4 dicarboxylic acids, improve fermentation conversion rate；The byproduct reactions such as magnesium carbonate and acetic acid, lactic acid, reduce the murder by poisoning to bacterial strain, mention
High fermentation efficiency；Magnesium ion is the activator of a variety of enzymes during bacterial strain synthesis glutamic acid, can stimulate strain growth and production
Acid；
The present invention adds inositol and magnesium carbonate in ferment middle, mutually cooperates with, improves fermentation efficiency.
Detailed description of the invention
Fig. 1: the schematic diagram of fermentation process of the present invention；
Fig. 2: film is coupled influence of the dialysis fermentation to Fungal biodiversity；
Fig. 3: influence of each factor to saccharic acid conversion ratio；
Fig. 4: influence of each factor to glutamic acid yield.
Specific embodiment
In order to make those skilled in the art better understand the technical solutions in the application, having below in conjunction with the application
The technical solution of the application is clearly and completely described in body embodiment, it is clear that described embodiment is only this Shen
Please a part of the embodiment, instead of all the embodiments.Based on the embodiment in the application, those of ordinary skill in the art are not having
Every other embodiment obtained under the premise of creative work is made, should fall within the scope of the present invention.
Embodiment 1
A method of it improving bacterial strain and produces sour efficiency comprising following steps:
Corynebacterium glutamicum ATCC13761 is inoculated into seed culture medium, at 33 DEG C, 100rpm shaking table culture 12h is obtained
Corynebacterium glutamicum seed liquor；The group of the Corynebacterium glutamicum seed culture medium is divided into (being below mass percent): Portugal
Grape sugar 6%, corn pulp 3%, potassium dihydrogen phosphate 0.2%, epsom salt 0.02%, manganese sulfate monohydrate 0.01%, 115 DEG C of sterilizings
15min；
By Corynebacterium glutamicum seed liquor with 10% inoculum concentration inoculation access equipped with 21L fermentation medium 30L fermentor in into
Row fermented and cultured, when fermentation is to 12h, adds 2g/L by 33 DEG C of fermentation temperature, ventilatory capacity 0.4vvm, speed of agitator 500r/min
Inositol and 1g/L magnesium carbonate, continue ferment 12h, the fermentation liquid in fermentor is pumped out via centrifugal pump, then through ceramics
UF membrane, obtains filtrate and concentration thallus, filtrate is drained into feed liquid storage tank, and concentration thallus is returned fermentor, while to hair
Fermentation tank culture medium is added in fermentation tank, make with without centrifugal pump processing before fermentating liquid volume it is identical, continue ferment 12h, then
The inositol of 2g/L and the magnesium carbonate of 1g/L are added, the 12h that ferments is continued, fermentation is completed, fermentation liquid is obtained, by the fermentation in fermentor
Liquid is pumped out via centrifugal pump, then through ceramic membrane separation, obtains filtrate and concentration thallus, filtrate is drained into feed liquid storage tank；It is whole
In a fermentation process, by stream plus GPE defoaming, while the pH value of Feeding ammonia water control fermentation liquid, to 6.0, stream plus glucose are molten
Hydraulic control residual sugar amount is not less than 1%；The molecular cut off of ceramic membrane is 20000Da；
The group of the fermentation tank culture medium is divided into (mass percent): glucose 12%, corn pulp 3%, urea 0.5%, biphosphate
Potassium 0.5%, ferrous sulfate heptahydrate 0.02%, manganese sulfate monohydrate 0.01%, fulvic acid 0.002%.
Embodiment 2
A method of it improving bacterial strain and produces sour efficiency comprising following steps:
Corynebacterium glutamicum ATCC13761 is inoculated into seed culture medium, at 33 DEG C, 100rpm shaking table culture 12h is obtained
Corynebacterium glutamicum seed liquor；The group of the Corynebacterium glutamicum seed culture medium is divided into (being below mass percent): Portugal
Grape sugar 6%, corn pulp 3%, potassium dihydrogen phosphate 0.2%, epsom salt 0.02%, manganese sulfate monohydrate 0.01%, 115 DEG C of sterilizings
15min；
Corynebacterium glutamicum seed liquor is equipped in the 30L fermentor of 21L fermentation medium with the inoculation access of 8% inoculum concentration and is carried out
Fermented and cultured, when fermentation is to 12h, adds 1g/L's by 33 DEG C of fermentation temperature, ventilatory capacity 0.4vvm, speed of agitator 500r/min
The magnesium carbonate of inositol and 0.5g/L continues the 12h that ferments, the fermentation liquid in fermentor is pumped out via centrifugal pump, then through ceramics
UF membrane, obtains filtrate and concentration thallus, filtrate is drained into feed liquid storage tank, and concentration thallus is returned fermentor, while to hair
Fermentation tank culture medium is added in fermentation tank, make with without centrifugal pump processing before fermentating liquid volume it is identical, continue ferment 12h, then
The inositol of 1g/L and the magnesium carbonate of 0.5g/L are added, the 12h that ferments is continued, fermentation is completed, fermentation liquid is obtained, by the hair in fermentor
Zymotic fluid is pumped out via centrifugal pump, then through ceramic membrane separation, obtains filtrate and concentration thallus, filtrate is drained into feed liquid storage tank；
In entire fermentation process, add GPE defoaming by flowing, while the pH value of Feeding ammonia water control fermentation liquid, to 5.5, stream adds glucose
Solution controls residual sugar amount and is not less than 1%；The molecular cut off of ceramic membrane is 10000Da；
The group of the fermentation tank culture medium is divided into (mass percent): glucose 12%, corn pulp 3%, urea 0.5%, biphosphate
Potassium 0.5%, ferrous sulfate heptahydrate 0.02%, manganese sulfate monohydrate 0.01%, fulvic acid 0.002%.
Embodiment 3
Analysis method
Thallus assay: OD600nm。
Glutamic acid measurement: using the multi-functional glutamic acid of SBA-40C-glucose analyser measurement.
Residual glucose: it is measured with Fehling method.
PH value measurement: pH determination of electrode is used.
Control group uses normal fermentation mode, culture medium and parameter with experimental group (embodiment 1) unanimously, fermentation time 36h.
Film is coupled influence of the dialysis fermentation to Fungal biodiversity
In glutamic acid fermentation production process, there is also close relationships for biomass and production acid, and relatively high biomass can
Secrete more glutamic acid.As shown in Figure 2, before carrying out dialysis fermentation (starting to dialyse for 24 hours) for 24 hours in, normal fermentation and
The OD of dialysis fermentation600nmValue is continuously increased, and reaches maximum value (53 or so) to fermentation 18h.For control fermentation, after 18h
OD600nmStart slowly decline, for 24 hours after then rapid decrease, reason be mainly nutrients shortage and toxic metabolite
Inhibiting effect.Corresponding dialysis fermentation then completely on the contrary, with dialysis progress, the removal of harmful toxic matter and nutrients
Addition so that thallus starts to increase, the OD of thallus600nmValue starts to be continuously increased again, until 36h reaches maximum (63.7), later
Start to be slowly drop down to fermentation ends.It can be seen that dialysis fermentation is to the separation of metabolism harmful toxic matter etc. and adding for nutriment
Add, can effectively improve the growth vigor of thallus, increases biomass.By calculating the yield of glutamic acid, dialysis fermentation is
154.3g/L, and normal fermentation is 133.6g/L, improves 15.5 percentage points.
Embodiment 4
Influence of each factor to conversion ratio and glutamic acid yield:
Control group is set, in which:
Control, 1: inositol and magnesium carbonate are not added in fermentation process, remaining is the same as embodiment 1；
Control 2: magnesium carbonate is not added in fermentation process, remaining is the same as embodiment 1；
Control 3: fermentation medium does not add fulvic acid, remaining is the same as embodiment 1；
Experimental group is embodiment 1.The saccharic acid conversion ratio and aminoglutaric acid concentration of each group are shown in Fig. 3-4, compared with compareing 1-3, experimental group
Saccharic acid conversion ratio and glutamic acid yield reach highest.Suitable fulvic acid is added in fermentation medium, can reduce by-product
The production quantity of object lactic acid and acetic acid, and then improve the yield of glutamic acid；And inositol can strengthen the fixed reaction of CO2, with carbonic acid
Magnesium mutually cooperates with, and saccharic acid conversion ratio can be improved, and reduces murder by poisoning of the by-product to bacterial strain, improves glutamic acid yield.
The above is only a preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, various improvements and modifications may be made without departing from the principle of the present invention, these improvements and modifications are also answered
It is considered as protection scope of the present invention.
Claims (7)
1. a kind of method for improving bacterial strain and producing sour efficiency uses film coupling dialysis batch fermentation.
2. the method according to claim 1, wherein film coupling dialysis batch fermentation is two Batch fermentations.
3. method according to claim 1 or 2, which is characterized in that described method includes following steps:
By Corynebacterium glutamicum seed liquor with the inoculation access of 6-10% inoculum concentration equipped in the 30L fermentor of 21L fermentation medium
Fermented and cultured is carried out, 33 DEG C of fermentation temperature, ventilatory capacity 0.4vvm, speed of agitator 500r/min, when fermentation is to 12h, adds 1-
The inositol of 2g/L and the magnesium carbonate of 0.5-1g/L continue the 12h that ferments, the fermentation liquid in fermentor are pumped out via centrifugal pump, so
By ceramic membrane separations, filtrate and concentration thallus are obtained, filtrate is drained into feed liquid storage tank, concentration thallus is returned fermentor,
Fermentation medium is added into fermentor simultaneously, continues the 12h that ferments, then add the inositol of 1-2g/L and the carbonic acid of 0.5-1g/L
Magnesium continues the 12h that ferments, and completes fermentation, obtains fermentation liquid, the fermentation liquid in fermentor is pumped out via centrifugal pump, then through making pottery
Porcelain UF membrane obtains filtrate and concentration thallus, filtrate is drained into feed liquid storage tank；In entire fermentation process, pass through stream plus GPE
Defoaming, while the pH value of Feeding ammonia water control fermentation liquid is to 5.5-6.0.
4. according to the method described in claim 3, it is characterized in that, the group of the fermentation medium is divided into (mass percent):
Glucose 12%, corn pulp 3%, urea 0.5%, potassium dihydrogen phosphate 0.5%, ferrous sulfate heptahydrate 0.02%, manganese sulfate monohydrate 0.01%,
Fulvic acid 0.002%.
5. according to the method described in claim 3, it is characterized in that, the molecular cut off of the ceramic membrane is 10000-
20000Da。
6. according to the method described in claim 3, it is characterized in that, the preparation process of the Corynebacterium glutamicum seed liquor are as follows:
Corynebacterium glutamicum is inoculated into seed culture medium, at 33 DEG C, 100rpm shaking table culture 12h, obtained glutamic acid rod
Bacillus seed liquor.
7. according to the method described in claim 6, it is characterized in that, it (is quality that the group of the seed culture medium is divided into below
Percentage): glucose 6%, corn pulp 3%, potassium dihydrogen phosphate 0.2%, epsom salt 0.02%, manganese sulfate monohydrate
0.01%, 115 DEG C of sterilizing 15min.
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Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20090269812A1 (en) * | 2006-02-24 | 2009-10-29 | Toray Industries, Inc , A Corporation Of Japan | Method of producing chemical product and continuous fermentation apparatus |
| JP2009254323A (en) * | 2008-04-21 | 2009-11-05 | Ajinomoto Co Inc | Method for producing l-glutamic acid-based amino acid |
| US20130330787A1 (en) * | 2010-12-09 | 2013-12-12 | Satoko Kanamori | Method for producing chemical by continuous fermentation |
| CN107227324A (en) * | 2017-08-07 | 2017-10-03 | 天津科技大学 | A kind of glutamic acid fermentation technique |
| CN109371072A (en) * | 2018-10-18 | 2019-02-22 | 许传高 | A kind of technique for reducing glutamic acid fermentation microbiological contamination and improving fermentation efficiency |
| CN109439703A (en) * | 2018-12-26 | 2019-03-08 | 齐齐哈尔龙江阜丰生物科技有限公司 | A kind of culture medium for Threonine Fermentation technique |
| CN109943604A (en) * | 2019-04-12 | 2019-06-28 | 卢松 | A method of improving glutamic acid fermentation conversion ratio and recovery rate |
-
2019
- 2019-03-18 CN CN201910201189.3A patent/CN110129385A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20090269812A1 (en) * | 2006-02-24 | 2009-10-29 | Toray Industries, Inc , A Corporation Of Japan | Method of producing chemical product and continuous fermentation apparatus |
| JP2009254323A (en) * | 2008-04-21 | 2009-11-05 | Ajinomoto Co Inc | Method for producing l-glutamic acid-based amino acid |
| US20130330787A1 (en) * | 2010-12-09 | 2013-12-12 | Satoko Kanamori | Method for producing chemical by continuous fermentation |
| CN107227324A (en) * | 2017-08-07 | 2017-10-03 | 天津科技大学 | A kind of glutamic acid fermentation technique |
| CN109371072A (en) * | 2018-10-18 | 2019-02-22 | 许传高 | A kind of technique for reducing glutamic acid fermentation microbiological contamination and improving fermentation efficiency |
| CN109439703A (en) * | 2018-12-26 | 2019-03-08 | 齐齐哈尔龙江阜丰生物科技有限公司 | A kind of culture medium for Threonine Fermentation technique |
| CN109943604A (en) * | 2019-04-12 | 2019-06-28 | 卢松 | A method of improving glutamic acid fermentation conversion ratio and recovery rate |
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Application publication date: 20190816 |