CN110129230A - A kind of microorganism formulation and its application in the sanitary sewage disposal of Expressway Service - Google Patents
A kind of microorganism formulation and its application in the sanitary sewage disposal of Expressway Service Download PDFInfo
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- CN110129230A CN110129230A CN201910416782.XA CN201910416782A CN110129230A CN 110129230 A CN110129230 A CN 110129230A CN 201910416782 A CN201910416782 A CN 201910416782A CN 110129230 A CN110129230 A CN 110129230A
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- microorganism formulation
- bacillus
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- bacillus licheniformis
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
Abstract
A kind of microorganism formulation, the microorganism formulation is prepared by following strain combination becomes composite bacteria agent: bacillus subtilis CCTCC NO:M 2011443, bacillus licheniformis CCTCC NO:M 2012457, Te Jila bacillus CCTCC NO:M 2014004, bacillus coagulans CCTCC NO:M 2015273, bacillus licheniformis CCTCC NO:M 2012458.Biological agent of the present invention realizes water quality reaching standard discharge for the pollution factor in high-speed service area sewage of degrading, and has the advantages that starting is rapid, strong shock resistance, regulation effect are good.
Description
Technical field
The present invention relates to field of environment engineering technology, more particularly, to a kind of microorganism formulation and its in freeway service
Application in area's sanitary sewage disposal.
Background technique
Expressway Service key facility has food and drink, lodging, toilet, supermarket, gas station.Expressway Service is dirty
Water is generally made of sanitary sewage, restaurant wastewater, carwash, gas station's sewage etc..Sewage is with sanitary sewage, gas station's cleaning sullage
Based on, be mainly characterized by: ammonia-nitrogen content is high, and coloration is high, and COD is big, and the pollutant loads such as phosphorus, heavy metal, greasy dirt are high,
Water quality is complicated, water fluctuation is big.This has much relations with the volume of traffic.The permanent personnel of service area are few, and mainly dealing vehicle passenger stops
By generating sewage.The dealing of vehicle has very big randomness.Therefore, the water of generation also has biggish fluctuation, water
When per, daily, be monthly likely to difference.Under normal conditions, Expressway Service is far from city planting ductwork, service area sewage without
Method is discharged into municipal sewer pipe network and is uniformly processed, it is necessary to individually build sewage treatment facility, " urban wastewater treatment firm is pressed in sewage discharge
Pollutant emission standard " (GB8978-2002) execution.
In the used sewage treatment process in Expressway Service and equipment, at the buried sewage of integral combination type
It is the most extensive to manage equipment application.Based on A/O technique, core process is catalytic oxidation for it, is to utilize the highly concentrated dirt of microbial degradation
Pollution factor in water realizes water quality reaching standard (Xu Beiqing, the comparison of Wastewater Treatment in Expressway Service Center technique, highway communication
Scientific and technological (application technology version), 9 phases in 2016: 213-215).In sewage treatment, the water outlets such as kitchen food and drink, carwash are carried out first
Oil removal processing, then collaborates dirty, waste water, is sent into conditioning tank after the pretreatment by septic tank and grid early period, adjusts water
It after matter and water, is handled by " pond A " (anaerobism), " pond O " (aerobic), sedimentation basin, clear water reserviors etc., realizes water quality reaching standard.
Sludge used is usually obtained by sewage plant activated sludge acclimatization in service area sewage treatment A/O technique.Activated sludge
Include pseudomonad (Pseudomonas), acinetobacter calcoaceticus (Acinetobacter), bacillus (Bacillus), Aeromonas
(Aeromaonas) multiple-microorganisms, complicated composition (Yang Qian, Jiang Yangyue, the Wang Xiaojun, etc. different zones sewage treatment plant is living such as
Property sludge in microbe colony structural analysis, chemical industry progress, the 12nd phase of volume 33 in 2014: 3329-3336).
The good activated sludge of regulation effect, microorganism formulation are always industry research hotspot.CN109082393A is disclosed
It is a kind of using can Treating Municipal Sewage microorganism formulation and preparation method thereof.Complex micro organism fungicide includes bacillus subtilis
More than 20 microorganism such as bacterium, bacillus licheniformis；Microbial bacteria agent carrier is by diatomite, crystal aluminum chloride, polyvinyl alcohol, seaweed
Sour calcium, maleic anhydride composition；Microbe carrier, complex micro organism fungicide, polyethylene glycol, chitosan etc. mix simultaneously in proportion
Balling-up is condensed, the microorganism formulation that can be used for Treating Municipal Sewage is obtained.CN105502684B is disclosed at a kind of composite flora
The method for managing high ammonia-nitrogen wastewater.Rhodopseudomonas palustris, Rhodospirillum rubrum, bacteroid, Fusobacterium are mixed to get composite flora
Aqueous solution, then composite flora aqueous solution is added into high ammonia-nitrogen wastewater；Under suitable temperature, light source, dissolved oxygen condition, COD, ammonia nitrogen
It is degraded.CN105331565A discloses a kind of microbial bacterial agent for purifying domestic sewage, and microbial inoculum includes denitrogenation sulphur bar
More than 10 microorganism such as bacterium, bacillus subtilis, bacillus licheniformis.It was tested through 3 months, sewage treatment is up to standard.
CN106085913B discloses a kind of complex microorganism preparations for sewage treatment, and said preparation is by Bdellovibrio, bacillus subtilis
Bacterium, thiobacillus denitrificans composition, to N, P, S significant effect in removal sewage.CN103421692B discloses reinforcing activated sludge
Composite microorganism viable bacteria preparation and its preparation method and application.The composite microorganism viable bacteria preparation is in paper-making effluent treating process
It is middle as activated sludge degrade COD hardening agent, can effectively, rapidly, strengthen activated sludge performance, efficient degradation paper waste
COD improves Colority of Sewage Water and water quality.
The daily blowdown flow rate in Expressway Service is different, especially festivals or holidays and tourist season and blowdown flow rate phase usually
Difference is very big, and according to large-scale sewage treatment facility, operating cost is high, and there may be activated sludge battalion in blowdown flow rate hour
It supports supply deficiency and causes microorganism dead, activated sludge failure；According to small-scale sewage treatment facility, then peak period
When, active sludge treatment ability is inadequate, and sewage discharge cannot be up to standard.The particularity of Expressway Service, to microorganism formulation
Performance more stringent requirements are proposed.
Summary of the invention
In view of the above-mentioned problems existing in the prior art, the applicant provides a kind of microorganism formulation and its in high speed
Application in the sanitary sewage disposal of high road service area.Microbial bacterial agent of the present invention, which has, starts rapid, strong shock resistance, improvement
The good advantage of effect.
Technical scheme is as follows:
A kind of microorganism formulation, the microorganism formulation is prepared by following strain combination becomes composite bacteria agent: withered grass gemma
Bacillus CCTCC NO:M 2011443, bacillus licheniformis CCTCC NO:M 2012457, Te Jila bacillus CCTCC NO:
M 2014004, bacillus coagulans CCTCC NO:M 2015273, bacillus licheniformis CCTCC NO:M 2012458.
The constituent of each strain is as follows in the microorganism formulation:
Every gram of product of strain corresponds to strain viable count (hundred million cfu/g)
2011443 10-30 of bacillus subtilis CCTCC NO:M
2012457 10-20 of bacillus licheniformis CCTCC NO:M
2014004 5-20 of Te Jila bacillus CCTCC NO:M
2015273 2-10 of bacillus coagulans CCTCC NO:M
2012458 2-10 of bacillus licheniformis CCTCC NO:M.
The bacillus subtilis (Bacillus subtilis), is preserved in China typical culture collection center, preservation
Number is CCTCC NO:M 2011443.
The bacillus licheniformis (Bacillus licheniformis), is preserved in China typical culture collection
The heart, deposit number are CCTCC NO:M 2012457.
The Te Jila bacillus is (BaciIlus tequiIensis), is preserved in China typical culture collection
The heart, deposit number are CCTCC NO:M 2014004.
The bacillus coagulans are (Bacillus coagulans), are preserved in China typical culture collection center,
Deposit number is CCTCC NO:M 2015273.
The bacillus licheniformis (Bacillus licheniformis), is preserved in China typical culture collection
The heart, deposit number are CCTCC NO:M 2012458.
It further include auxiliary material in the microorganism formulation, the auxiliary material is talcum powder and/or wheat bran.
A kind of application of the microorganism formulation, the microorganism formulation are dirty for handling Expressway Service life
Water.
The present invention is beneficial to be had the technical effect that
Biological agent of the present invention realizes water quality reaching standard discharge for the pollution factor in high-speed service area sewage of degrading.This
The invention starting time only needs 3-5 days；Strong shock resistance；Stable water outlet is up to standard；And maintenance is simple, only needs regular replenishment microorganism
Preparation.
Detailed description of the invention
Fig. 1 is that present invention water outlet pH changes with time；
Fig. 2 is that present invention water outlet COD changes with time；
Fig. 3 is present invention water outlet BOD5It changes with time；
Fig. 4 is that present invention water outlet SS changes with time；
Fig. 5 is that present invention water outlet total nitrogen changes with time；
Fig. 6 is that present invention water outlet ammonia nitrogen changes with time；
Fig. 7 is that present invention water outlet total phosphorus changes with time；
Fig. 8 is series model schematic diagram of the present invention；
Fig. 9 is paralleling model schematic diagram of the present invention.
Specific embodiment
Below with reference to embodiment, the present invention is specifically described.
Bacillus subtilis n-1 (Bacillus subtilis n-1) of the present invention was preserved on December 4th, 2011 to be located at
The China typical culture collection center of Wuhan, China, deposit number are CCTCC NO:M 2011443.
Bacillus licheniformis BL-1 (Bacillus licheniformis BL-1) is preserved in position on November 14th, 2012
In the China typical culture collection center of Wuhan, China, deposit number is CCTCC NO:M 2012457.
Te Jila bacillus ZSGD5 (BaciIlus tequiIensis ZSGD5) is preserved in position on January 6th, 2014
In the China typical culture collection center of Wuhan, China, deposit number is CCTCC NO:M 2014004.
Bacillus coagulans ZS-007 (BaciIlus tequiIensis ZS-007) is preserved in position on May 3rd, 2015
In the China typical culture collection center of Wuhan, China, deposit number is CCTCC NO:M 2015273.
Bacillus licheniformis BL-2 (Bacillus licheniformis BL-2) is preserved in position on November 14th, 2012
In the China typical culture collection center of Wuhan, China, deposit number is CCTCC NO:M 2012458.
Examples 1 to 5
When preparing microorganism formulation, microorganism single bacterium powder is uniformly mixed with auxiliary material and obtains finished product, embodiment bacteria preparation
Composition is shown in Table 1.
Table 1
Test case:
Waste water control test is carried out in Hubei high-speed service area, the time is in August, 2018 in March, -2019.The service area
200 ton/days of the daily sewage production quantity of sewage, festivals or holidays increase 50%-200%.In sewage, COD 350-550mg/L, BOD5
150-300mg/L, SS 100-200mg/L, total nitrogen 100-180mg/L, ammonia nitrogen 50-90mg/L, total phosphorus 5-15mg/L.
It is handled using combined-type sewage treatment facility.Capital equipment situation is as follows: collecting-tank, 25.2m3；Conditioning tank,
49.06m3, composite filling is partially mounted in conditioning tank；The pond A, 29.44m3, composite filling and aeration head aeration are installed in pond
Pipe, it is uninterrupted to be aerated；The pond O, 49.1m3, composite filling and aeration head aeration tube are installed in pond, it is uninterrupted to be aerated；Secondary settling tank,
19.62m3, inclined tube filler is installed in pond；There is pipeline connection in conditioning tank and the pond A, the pond O；There is pipeline company in secondary settling tank and the pond A, the pond O
It connects.
Sewage treatment is carried out using series connection (Fig. 8) and (Fig. 9) in parallel 2 kinds of modes.Wherein, simultaneously gang mould is used under even in everyday situations
Formula；The stream of people, which increases sharply first 3 days, switches to series model, switches back into paralleling model again after the stream of people restores normal level.
Use the microorganism formulation of embodiment 1.Preparation is poured into bucket after adding clear water to stir evenly, pours into the pond A, O starts in pond
Bored aeration adds preparation 100g daily, and gradually intakes to full-water level for first 3 days；After three days, system starts normally to intake, aeration
To be interrupted, and preparation 100g is added to the pond A, the pond O daily, until the 5th finishes；It is subsequent to add system to the pond A, the pond O every 7-10 days
Agent 100g.Sewage disposal system automatic running uses series model during " 11 ", the Spring Festival, remaining time uses paralleling model.
Effluent quality (pH, COD, BOD are analyzed in periodically sampling5, SS, total nitrogen, ammonia nitrogen, total phosphorus), analysis method press GB8978-2002.Point
Analysis data are respectively as shown in Fig. 1~7.
It can be seen from the data of Fig. 1~7 in more than 200 day operational process, pH6.5-8.0, COD 39-49mg/L, total nitrogen
10-15mg/L, ammonia nitrogen 1.7-4.9mg/L；BOD5Only initially reaching 13mg/L, remaining time is below 10mg/L；SS only exists
Reach within first day 15mg/L, is lower than 10mg/L after operation 14 days；Total phosphorus only reached 1.2mg/L at first day, low after operation 14 days
In 0.5mg/L." 11 " and spring transportation stream of people surge situation and winter low temperature situation are undergone during test, but effluent quality is still steady
It is fixed, reach GB8978-2002 level-one A standard.
Using microbial inoculum and treatment process of the invention, the beneficial effect is that: (1) pond " A ", the pond " O " are in interval and expose
Under gaseity, anaerobism, Aerobic Process for Treatment stage are not repartitioned, and one step of decomposition of pollution factor is completed, and more previous technique is greatly simplified；
(2) paralleling model that normal person flows down can make microorganism maintain working condition, be not in that " nutrition supply is insufficient and leads to micro- life
Object is dead " the phenomenon that；(3) sewage disposal system can enter series model within a short period of time, to cope with stream of people's surge situation；
(4) series and parallel operational mode need to only carry out pipeline laying etc. on the basis of existing device and simply be transformed, and maximizing is using now
There are facility, investment reduction.In conclusion the microorganism formulation and treatment process of the present patent application, the starting time is short, shock resistance energy
Power is strong, stable water outlet is up to standard, maintenance is simple.
Claims (9)
1. a kind of microorganism formulation, which is characterized in that the microorganism formulation is prepared by following strain combination becomes composite bacteria agent:
Bacillus subtilis CCTCC NO:M 2011443, bacillus licheniformis CCTCC NO:M 2012457, Te Jila bacillus
CCTCC NO:M 2014004, bacillus coagulans CCTCC NO:M 2015273, bacillus licheniformis CCTCC NO:M
2012458。
2. microorganism formulation according to claim 1, which is characterized in that in the microorganism formulation composition of each strain at
Divide as follows:
3. microorganism formulation according to claim 1, which is characterized in that the bacillus subtilis (Bacillus
Subtilis), it is preserved in China typical culture collection center, deposit number is CCTCC NO:M 2011443.
4. microorganism formulation according to claim 1, which is characterized in that the bacillus licheniformis (Bacillus
Licheniformis), it is preserved in China typical culture collection center, deposit number is CCTCC NO:M 2012457.
5. microorganism formulation according to claim 1, which is characterized in that the Te Jila bacillus is (BaciIlus
TequiIensis), it is preserved in China typical culture collection center, deposit number is CCTCC NO:M 2014004.
6. microorganism formulation according to claim 1, which is characterized in that the bacillus coagulans are (Bacillus
Coagulans), it is preserved in China typical culture collection center, deposit number is CCTCC NO:M 2015273.
7. microorganism formulation according to claim 1, which is characterized in that the bacillus licheniformis (Bacillus
Licheniformis), it is preserved in China typical culture collection center, deposit number is CCTCC NO:M 2012458.
8. microorganism formulation according to claim 1, which is characterized in that it further include auxiliary material in the microorganism formulation, institute
Stating auxiliary material is talcum powder and/or wheat bran.
9. a kind of application of microorganism formulation described in claim 1, which is characterized in that the microorganism formulation is for handling high speed
High road service area sanitary sewage.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN115806349A (en) * | 2022-12-15 | 2023-03-17 | 鹤山市新的生物制品有限公司 | Method for increasing dissolved oxygen content of aquaculture water and special culture medium |
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