CN110129230B - Microbial preparation and application thereof in domestic sewage treatment of expressway service area - Google Patents
Microbial preparation and application thereof in domestic sewage treatment of expressway service area Download PDFInfo
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- CN110129230B CN110129230B CN201910416782.XA CN201910416782A CN110129230B CN 110129230 B CN110129230 B CN 110129230B CN 201910416782 A CN201910416782 A CN 201910416782A CN 110129230 B CN110129230 B CN 110129230B
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
Abstract
A microbial preparation is prepared by combining the following strains into a composite microbial inoculum: bacillus subtilis CCTCC NO: m2011443, Bacillus licheniformis CCTCC NO: m2012457, Bacillus tequilensis CCTCC NO: m2014004, Bacillus coagulans CCTCC NO: m2015273, Bacillus licheniformis CCTCC NO: m2012458. The biological agent is used for degrading pollution factors in sewage in a high-speed service area, realizes standard discharge of water quality, and has the advantages of quick start, strong impact resistance and good treatment effect.
Description
Technical Field
The invention relates to the technical field of environmental engineering, in particular to a microbial preparation and application thereof in domestic sewage treatment of a highway service area.
Background
The main facilities of the expressway service area comprise catering, accommodation, washrooms, supermarkets and gas stations. The sewage of the expressway service area generally comprises domestic sewage, restaurant sewage, car washing sewage, gas station sewage and the like. The sewage is mainly domestic sewage and cleaning sewage of gas stations, and is mainly characterized in that: high ammonia nitrogen content, high chroma, large chemical oxygen demand, high contents of pollutants such as phosphorus, heavy metals, oil stains and the like, complex water quality and large water fluctuation. This is strongly related to traffic volume. The service area has few standing personnel, and mainly the passengers coming to and going to the vehicle stop to generate sewage. The traffic of the vehicle has great randomness. Therefore, the amount of water generated also has a large fluctuation, and the amount of water may vary every time, every day, and every month. In general, a service area of the expressway is far away from a municipal pipe network, sewage in the service area cannot be discharged into the municipal sewer network for uniform treatment, a sewage treatment facility must be separately constructed, and the sewage discharge is implemented according to the discharge standard of pollutants for municipal sewage treatment plants (GB 8978-2002).
Among the sewage treatment processes and equipment used in expressway service areas, integrated combined underground sewage treatment equipment is most widely applied. The method mainly adopts an A/O process, adopts a core process of contact oxidation, and utilizes microorganisms to degrade pollution factors in high-concentration sewage to realize the water quality reaching the standard (Xubeiqing, comparison of sewage treatment processes in expressway service areas, highway traffic technology (application technology edition), 2016 (9 th year: 213-) -215). In the sewage treatment, firstly, effluent of catering, car washing and the like in a kitchen is subjected to oil separation treatment, then sewage and wastewater are converged and sent into an adjusting tank after pretreatment of a septic tank and a grid in the early stage, and after water quality and water quantity are adjusted, the water quality reaches the standard through treatment of an A tank (anaerobic), an O tank (aerobic), a sedimentation tank, a clean water tank and the like.
The sludge used in the service area sewage treatment a/O process is typically acclimated from sewage plant activated sludge. The activated sludge contains various microorganisms such as Pseudomonas (Pseudomonas), Acinetobacter (Acinetobacter), Bacillus (Bacillus), aeromonas (Aeromonas) and the like, and has complex composition (Naqian, Jianyang month, Wang Xiaojun, and the like, structural analysis of microbial colonies in activated sludge of sewage treatment plants in different areas, chemical development, No. 33, No. 12 in 2014: 3329-3336).
Activated sludge and microbial agents with good treatment effect are always hot research hotspots in the industry. CN109082393A discloses a microbial preparation for treating municipal sewage and a preparation method thereof. The compound microbial agent contains more than 20 kinds of microorganisms such as bacillus subtilis, bacillus licheniformis and the like; the microbial agent carrier consists of diatomite, crystalline aluminum chloride, polyvinyl alcohol, calcium alginate and maleic anhydride; mixing the microbial carrier, the composite microbial agent, the polyethylene glycol, the chitosan and the like in proportion and coagulating into balls to obtain the microbial preparation for treating the urban sewage. CN105502684B discloses a method for treating high ammonia nitrogen wastewater by using composite flora. Mixing rhodopseudomonas palustris, rhodospirillum rubrum, bacteroides and clostridium to obtain a composite flora aqueous solution, and adding the composite flora aqueous solution into high ammonia nitrogen wastewater; under the conditions of proper temperature, light source and dissolved oxygen, COD and ammonia nitrogen are degraded. CN105331565A discloses a microbial agent for purifying domestic sewage, which comprises more than 10 kinds of microorganisms such as thiobacillus denitrificans, bacillus subtilis, bacillus licheniformis and the like. After 3 months of experiments, the sewage treatment reaches the standard. CN106085913B discloses a compound microbial preparation for sewage treatment, which is composed of bdellovibrio bacteriovorus, bacillus subtilis and thiobacillus denitrificans and has an obvious effect of removing N, P, S in sewage. CN103421692B discloses a composite microorganism live bacteria preparation for strengthening activated sludge, a preparation method and application thereof. The composite microorganism live bacteria preparation is used as an activated sludge degradation COD enhancer in the papermaking wastewater treatment process, and can effectively, rapidly and strongly enhance the performance of activated sludge, efficiently degrade the COD of papermaking wastewater and improve the chroma and the water quality of sewage.
The daily discharge capacity of the expressway service area is different, particularly, the discharge capacity in holidays and busy traveling seasons is greatly different from that in normal times, if a large-scale sewage treatment facility is adopted, the operation cost is high, and the shortage of activated sludge nutrition supply can be generated when the discharge capacity is small, so that the microorganisms die, and the activated sludge is ineffective; if a small-scale sewage treatment facility is adopted, the activated sludge treatment capacity is insufficient during the peak period, and the sewage discharge can not reach the standard. The particularity of the service area of the expressway puts higher requirements on the performance of microbial agents.
Disclosure of Invention
In view of the above problems in the prior art, the applicant of the present invention provides a microbial preparation and an application thereof in domestic sewage treatment in a highway service area. The microbial agent has the advantages of quick start, strong impact resistance and good treatment effect.
The technical scheme of the invention is as follows:
a microbial preparation is prepared by combining the following strains into a composite microbial inoculum: bacillus subtilis CCTCC NO: m2011443, Bacillus licheniformis CCTCC NO: m2012457, Bacillus tequilensis CCTCC NO: m2014004, Bacillus coagulans CCTCC NO: m2015273, Bacillus licheniformis CCTCC NO: m2012458.
The microbial preparation comprises the following components of various strains:
one gram of strain product corresponds to the viable count of strain (hundred million cfu/g)
Bacillus subtilis CCTCC NO: m201144310-30
Bacillus licheniformis CCTCC NO: m201245710-20
Bacillus tequilensis CCTCC NO: m20140045-20
Bacillus coagulans CCTCC NO: m20152732-10
Bacillus licheniformis CCTCC NO: m20124582-10.
The Bacillus subtilis is preserved in China center for type culture Collection with the preservation number of CCTCC NO: m2011443.
The Bacillus licheniformis (Bacillus licheniformis) is preserved in China center for type culture Collection, and the preservation number is CCTCC NO: m2012457.
The bacillus tequilensis is (BaciIlus tequiensis) and is preserved in China center for type culture Collection, and the preservation number is CCTCC NO: m2014004.
The Bacillus coagulans is (Bacillus coagulans) and is preserved in China center for type culture collection with the preservation number of CCTCC NO: m2015273.
The Bacillus licheniformis (Bacillus licheniformis) is preserved in China center for type culture Collection, and the preservation number is CCTCC NO: m2012458.
The microbial preparation also comprises an auxiliary material, wherein the auxiliary material is talcum powder and/or bran.
The application of the microbial preparation is to treat domestic sewage in a highway service area.
The beneficial technical effects of the invention are as follows:
the biological agent is used for degrading pollution factors in sewage in a high-speed service area and realizing the standard discharge of water quality. The starting time of the invention only needs 3 to 5 days; the shock resistance is strong; the effluent is stable and reaches the standard; and the maintenance is simple, and only the microbial preparation needs to be supplemented periodically.
Drawings
FIG. 1 is a graph of the present invention showing the change of pH of the effluent over time;
FIG. 2 is the change of effluent COD over time according to the present invention;
FIG. 3 shows the effluent BOD of the present invention5A change over time;
FIG. 4 is a graph of effluent SS over time in accordance with the present invention;
FIG. 5 is a graph of the total nitrogen of the effluent over time in accordance with the present invention;
FIG. 6 shows the ammonia nitrogen in the effluent water varying with time according to the present invention;
FIG. 7 is a graph of the total phosphorus of the effluent over time in accordance with the present invention;
FIG. 8 is a schematic diagram of a series mode of the present invention;
FIG. 9 is a schematic diagram of a parallel mode of the present invention.
Detailed Description
The present invention will be described in detail with reference to examples.
The Bacillus subtilis n-1(Bacillus subtilis n-1) is preserved in the China Center for Type Culture Collection (CCTCC) in Wuhan, 2011, 12 and 4, with the preservation number of CCTCC NO: m2011443.
Bacillus licheniformis BL-1(Bacillus licheniformis BL-1) is preserved in China center for type culture Collection in Wuhan, China in 2012 at 11/14, with the preservation number of CCTCC NO: m2012457.
The bacillus tequilensis ZSGD5(BaciIlus tequiensis ZSGD5) is preserved in China center for type culture Collection in Wuhan, China in 1 month and 6 days 2014, and the preservation number is CCTCC NO: m2014004.
Bacillus coagulans ZS-007(BaciIlus tequiIensis ZS-007) was deposited in the China center for type culture Collection in Wuhan, China at 5 months and 3 days 2015 with the deposition number of CCTCC NO: m2015273.
Bacillus licheniformis BL-2(Bacillus licheniformis BL-2) is preserved in China center for type culture Collection in Wuhan, China in 2012 at 11/14, with the preservation number of CCTCC NO: m2012458.
Examples 1 to 5
When the microbial preparation is prepared, the single microbial powder and the auxiliary materials are uniformly mixed to obtain a finished product, and the compositions of the microbial preparations in the examples are shown in table 1.
TABLE 1
Test example:
and carrying out a sewage treatment test in a certain high-speed service area in Hubei for 8 months in 2018 to 3 months in 2019. The daily sewage generation amount of the sewage in the service area is 200 tons/day, and the holiday and festival are increased by 50-200%. COD 350-5150-300mg/L, SS 100-200mg/L, total nitrogen 100-180mg/L, ammonia nitrogen 50-90mg/L, total phosphorus 5-15 mg/L.
The sewage is treated by adopting a combined sewage treatment facility. The main equipment conditions are as follows: catch basin, 25.2m3(ii) a Equalizing basin, 49.06m3The part in the adjusting tank is provided with a combined filler; pool A, 29.44m3The tank is provided with a combined filler and an aeration head aeration pipe for uninterrupted aeration; o pool, 49.1m3The tank is provided with a combined filler and an aeration head aeration pipe for uninterrupted aeration; secondary sedimentation tank, 19.62m3The pool is provided with an inclined tube filler; the adjusting tank is connected with the tank A and the tank O through pipelines; the secondary sedimentation tank is connected with the A tank and the O tank through pipelines.
The sewage treatment is carried out by adopting 2 modes of serial connection (figure 8) and parallel connection (figure 9). Wherein, the parallel mode is used in daily situations; and 3 days before the people flow is increased rapidly, the mode is switched to the series mode, and the mode is switched back to the parallel mode after the people flow is restored to the normal level.
The microbial preparation of example 1 was used. Pouring the preparation into a barrel, adding clear water, stirring, pouring into tank A and tank O, aerating, adding 100g of the preparation every day in the first 3 days, and gradually adding water to full water level; after three days, the system starts normal water inflow, aeration is interrupted, and 100g of the preparation is added into the A pool and the O pool every day until the 5 th day; and then 100g of preparation is added into the tank A and the tank O every 7-10 days. The sewage treatment system automatically operates in a series mode in eleven spring festival period and a parallel mode in the rest time. Periodically sampling, and analyzing effluent quality (pH, COD, BOD)5SS, total nitrogen, ammonia nitrogen, total phosphorus), the analytical method is according to GB 8978-2002. The analytical data are shown in FIGS. 1 to 7, respectively.
As can be seen from the data in FIGS. 1-7, p is measured during the course of more than 200 days of operation6.5-8.0H, 39-49mg/L COD, 10-15mg/L total nitrogen and 1.7-4.9mg/L ammonia nitrogen; BOD5Only reaches 13mg/L initially and is lower than 10mg/L in the rest time; SS reaches 15mg/L only in the first day, and is lower than 10mg/L after 14 days of operation; total phosphorus reached 1.2mg/L only on day one and was less than 0.5mg/L after 14 days of operation. Eleven and spring transportation people flow rapidly increasing conditions and winter low-temperature conditions are experienced during the test period, but the effluent quality is still stable and reaches the GB8978-2002 first-grade A standard.
The microbial inoculum and the treatment process have the beneficial effects that: (1) the A tank and the O tank are in an intermittent aeration state, anaerobic and aerobic treatment stages are not distinguished, the decomposition of pollution factors is completed in one step, and the process is greatly simplified compared with the prior process; (2) the parallel mode under normal stream can maintain the working state of the microorganism, and the phenomenon of microorganism death caused by insufficient nutrition supply can not occur; (3) the sewage treatment system can enter a series mode in a short time so as to deal with the situation of rapid increase of people; (4) the serial and parallel operation modes only need to carry out simple reconstruction such as pipeline laying and the like on the basis of the existing equipment, so that the existing facilities can be utilized to the maximum extent, and the investment is saved. In conclusion, the microbial preparation and the treatment process disclosed by the invention have the advantages of short start-up time, strong impact resistance, stable effluent reaching the standard and simple maintenance.
Claims (4)
1. The microbial preparation is characterized by being prepared into a composite microbial agent by combining the following strains:
bacillus subtilis with a preservation number of CCTCC NO: m2011443,
Bacillus licheniformis (Bacillus licheniformis), the preservation number is CCTCC NO: m2012457, Bacillus tequilensis (BaciIlus tequiensis), with the preservation number of CCTCC NO: m2014004, Bacillus coagulans (Bacillus coagulans), the preservation number is CCTCC NO: m2015273, Bacillus licheniformis (Bacillus licheniformis), the preservation number is CCTCC NO: m2012458.
2. The microbial preparation of claim 1, wherein each species of the microbial preparation comprises the following components:
3. the microbial preparation according to claim 1, further comprising an adjuvant, wherein the adjuvant is talc and/or bran.
4. Use of the microbial preparation according to claim 1 for treating domestic sewage in a service area of a highway.
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| CN103045511B (en) * | 2012-12-18 | 2014-07-09 | 江南大学 | Cellulase production bacillus licheniformis with phosphate-solubilizing and potassium-releasing function and application of same |
| CN103045510B (en) * | 2012-12-18 | 2014-07-09 | 江南大学 | High-yield cellulase bacillus licheniformis with flocculation and application of same |
| CN104017757B (en) * | 2014-06-03 | 2016-05-18 | 江南大学 | The one plant height effect end, changed bacillus and the composite base made changes microbial inoculum and application |
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