CN110129227A - It is a kind of for removing the mix bacterium agent of heavy metal and its application in industrial wastewater sludge - Google Patents
It is a kind of for removing the mix bacterium agent of heavy metal and its application in industrial wastewater sludge Download PDFInfo
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F11/00—Treatment of sludge; Devices therefor
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- C02F2101/00—Nature of the contaminant
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Abstract
The invention discloses a kind of for removing the mix bacterium agent of heavy metal in industrial wastewater sludge, it not only include Thiobacillus thioxidans, Thiobacillus ferrooxidans, it further include aerobic denitrifying bacteria, resistance to heavy metal bacterium and extreme thiobacillus ferrooxidans, wherein the effect of aerobic denitrifying bacteria is that ammonium nitrogen is directly translated into gaseous products under aerobic condition, have and promote organic matter conversion, accelerates the effect of sludge acidification rate；The effect of resistance to heavy metal bacterium is to improve other bacteriums to the tolerance of heavy metal；And the effect of extreme thiobacillus ferrooxidans is under strongly acidic conditions, to be obviously improved the leaching efficiency of heavy metal in the solution, shortens the extraction time of heavy metal.Compared to conventional method, mix bacterium agent of the invention is not only obviously improved the removal effect of the heavy metals such as Cu, Cd, Hg, Cr and Pd in sludge, and removal efficiency is also obviously improved.
Description
Technical field
The present invention relates to field more particularly to it is a kind of for remove in industrial wastewater sludge the mix bacterium agent of heavy metal and its
Using.
Background technique
It is that recycling sludge utilizes most that Heavy Metals in Sludge is many kinds of, which mainly to have Cu, Pb, Zn, Ni, Cr, Hg and Cd etc.,
Major obstacle.To handle generated sludge based on sanitary sewage, content of beary metal is usually lower, and is to handle trade effluent
Sludge caused by main, content of beary metal are often higher.Due to heavy metal has the characteristics that difficult migration, be easily enriched with and endanger it is big
Main factor as limitation sludge agricultural use.Whether municipal sludge or industrial sludge, due to its contain mostly it is toxic
Property biggish heavy metal element, will be to sewage treatment plant or even using the regional periphery ecology of sewage sludge if dealt with improperly
Environment, which is constituted, to be seriously endangered.Therefore, reducing or remove the exceeded heavy metal element in sludge becomes effectively disposition and utilizes sludge
Key.Currently, to solve harm that heavy metals in city sewage sludge element may cause ecological environment and to agricultural sludge
The research carried out of restriction it is more, but majority is carried out from stablizing and reduce heavy metal element toxicity angle, from reducing dirt
The research of total metals is relatively fewer in mud.
Bioleaching is directly acted on using natural some microorganisms or the indirectly-acting of its metabolite, such as aoxidizes,
The effects of reduction, acidification, leaches the certain slightly solubilities of sludge or insoluble composition (such as heavy metal, sulphur) separation.Again by sludge
It is dehydrated, realizes the purpose of removal Heavy Metals in Sludge.Bioleaching has the advantage that compared with chemical leaching, can save
About acid consumption is not required to acid adding and carries out pre-acidified to sludge, can digest and combine with aerobic sludge, and existing operation is made full use of to set
It applies, reduces operating cost and capital cost；Rapidly, leaching is high-efficient, and the time is short for starting, is suitable for processing any type sludge；Operation
Simply, operational process is not necessarily to Special controlling, can leaching heavy metal within the scope of 10 DEG C -37 DEG C；Sludge takes off after bioleaching
Aqueous to greatly improve, when dehydration, does not need addition flocculating agent, effectively saves sludge dewatering cost；Bioleaching can effectively be gone
Removing heavy metals can kill pathogen again, and decline VSS.
Chinese invention patent CN105776788A discloses weight in a kind of bioleaching removal sludge from wastewater treatment plant
The method of Ni metal, this method carry out enrichment culture using Thiobacillus original in sludge and obtain acclimation sludge, and the most with this
Inoculum is added in primary sludge, and adds Na2S2O3And FeSO4Substrate, by the 3-4d bioleaching time, so that heavy metal
The removal rate of Cu reaches 90% or more.But this method is bad to the removal effect of other heavy metals such as Cd, Hg, Cr and Pd, together
When the bioleaching time time it is longer, therefore it is necessary to study a kind of mix bacterium agent, for being promoted in removal industrial wastewater sludge
The effect of heavy metal.
Summary of the invention
Technical problems based on background technology, the invention proposes one kind for removing a huge sum of money in industrial wastewater sludge
The mix bacterium agent of category.
Technical scheme is as follows:
It is a kind of for removing the mix bacterium agent of heavy metal in industrial wastewater sludge, including following component: Thiobacillus thioxidans,
Thiobacillus ferrooxidans, aerobic denitrifying bacteria, resistance to heavy metal bacterium and extreme thiobacillus ferrooxidans.
Preferably, the resistance to heavy metal bacterium is that P. aeruginosa disclosed in Chinese invention patent CN102337229A is thin
Bacterium ZGKD2.
Preferably, the extreme thiobacillus ferrooxidans are thiobacillus ferrooxidans disclosed in Chinese invention patent CN103131650A
Belong to ZJJN.
Preferably, Thiobacillus thioxidans, Thiobacillus ferrooxidans, aerobic denitrifying bacteria, resistance to heavy in the mix bacterium agent
The weight ratio of metal bacterium and extreme thiobacillus ferrooxidans are (3-5): (6-10): (4-10): (1-2): (2-5).
Input amount of the mix bacterium agent in industrial wastewater sludge is 0.05-0.2%.
The application method of the mix bacterium agent, comprising the following steps:
(1) first time enrichment culture: taking the primary sludge of sewage treatment plant, and the Na of 3-5g/L is added into primary sludge2S2O3With
The FeSO of 4-6g/L4For substrate, mix bacterium agent is added, is passed through nitrogen and enrichment culture to sludge pH is down to 3.5-4.0, obtains the
Acclimation sludge after enrichment culture；
(2) second of enrichment culture: the acclimation sludge after taking first time enrichment culture, 0.5-1% is inoculated by volume
In primary sludge, the Na of 3-5g/L is added2S2O3With the FeSO of 4-6g/L4For substrate, the enrichment culture under oxygen sufficiency, directly
It is down to 2.0-2.5 to pH value, the acclimation sludge after obtaining second of enrichment culture；Gained acclimation sludge mixed liquor is inoculum；
(3) bioleaching: using step (2) resulting inoculum, and 0.5-1% is inoculated into primary sludge by volume, addition
The Na of 3-5g/L2S2O3With the FeSO of 4-6g/L4For substrate, initial pH value is adjusted to 5-7, leaching culture at a temperature of 20-30 DEG C
The effects of 16-24h, Thiobacillus thioxidans and Thiobacillus ferrooxidans are restored by oxidation, acidification, by the huge sum of money in primary sludge
Belong to separation and is leached to water phase；
(4) sludge settling: the sludge settling after step (3) to be carried out to bioleaching obtains the sludge of low-heavy metal content
With the sewage of high content of beary metal.
The invention has the beneficial effects that: the mix bacterium agent for being used to remove heavy metal in industrial wastewater sludge of the invention,
Include not only Thiobacillus thioxidans, Thiobacillus ferrooxidans, further includes aerobic denitrifying bacteria, resistance to heavy metal bacterium and extreme acidophilus
Thiobacillus, wherein the effect of aerobic denitrifying bacteria is that ammonium nitrogen is directly translated into gaseous products under aerobic condition, there is promotion
Organic matter conversion, accelerates the effect of sludge acidification rate；The effect of resistance to heavy metal bacterium is to improve other bacteriums to heavy metal
Tolerance；And the effect of extreme thiobacillus ferrooxidans is under strongly acidic conditions, to be obviously improved the leaching efficiency of heavy metal in the solution,
Shorten the extraction time of heavy metal.
Specific embodiment
Embodiment 1:
It is a kind of for removing the mix bacterium agent of heavy metal in industrial wastewater sludge, including following component: Thiobacillus thioxidans,
Thiobacillus ferrooxidans, aerobic denitrifying bacteria, resistance to heavy metal bacterium and extreme thiobacillus ferrooxidans.
The resistance to heavy metal bacterium is P. aeruginosa bacterium disclosed in Chinese invention patent CN102337229A
ZGKD2。
The extreme thiobacillus ferrooxidans are that thiobacillus ferrooxidans disclosed in Chinese invention patent CN103131650A belong to ZJJN.
In the mix bacterium agent, Thiobacillus thioxidans, Thiobacillus ferrooxidans, aerobic denitrifying bacteria, resistance to heavy metal are thin
The weight ratio of bacterium and extreme thiobacillus ferrooxidans are 3.5:8:6:1.5:3.
Preferably, input amount of the mix bacterium agent in industrial wastewater sludge is 0.15%.
Preferably, the application method of the mix bacterium agent, comprising the following steps:
(1) first time enrichment culture: taking the primary sludge of sewage treatment plant, and the Na of 3.5g/L is added into primary sludge2S2O3With
The FeSO of 5g/L4For substrate, mix bacterium agent is added, is passed through nitrogen and enrichment culture to sludge pH is down to 3.5-4.0, obtain first
Acclimation sludge after secondary enrichment culture；
(2) second of enrichment culture: the acclimation sludge after taking first time enrichment culture 0.8% is inoculated into life by volume
In sludge, the Na of 3.5g/L is added2S2O3With the FeSO of 4.5g/L4For substrate, the enrichment culture under oxygen sufficiency, until
PH value is down to 2.0-2.5, the acclimation sludge after obtaining second of enrichment culture；Gained acclimation sludge mixed liquor is inoculum；
(3) bioleaching: using step (2) resulting inoculum, and 0.5-1% is inoculated into primary sludge by volume, addition
The Na of 4.5g/L2S2O3With the FeSO of 5.5g/L4For substrate, initial pH value is adjusted to 5-7, leaching culture 18h at a temperature of 25 DEG C,
The effects of Thiobacillus thioxidans and Thiobacillus ferrooxidans are restored by oxidation, acidification, separates leaching for the heavy metal in primary sludge
Out to water phase；
(4) sludge settling: the sludge settling after step (3) to be carried out to bioleaching obtains the sludge of low-heavy metal content
With the sewage of high content of beary metal.
The aeration intensity of the leaching culture of step (3) is controlled at 1.2L/ (minL)；The leaching culture of step (3) is stirred
Mixing revolving speed is 220r/min.
Embodiment 2:
It is a kind of for removing the mix bacterium agent of heavy metal in industrial wastewater sludge, including following component: Thiobacillus thioxidans,
Thiobacillus ferrooxidans, aerobic denitrifying bacteria, resistance to heavy metal bacterium and extreme thiobacillus ferrooxidans.
The resistance to heavy metal bacterium is P. aeruginosa bacterium disclosed in Chinese invention patent CN102337229A
ZGKD2。
The extreme thiobacillus ferrooxidans are that thiobacillus ferrooxidans disclosed in Chinese invention patent CN103131650A belong to ZJJN.
In the mix bacterium agent, Thiobacillus thioxidans, Thiobacillus ferrooxidans, aerobic denitrifying bacteria, resistance to heavy metal are thin
The weight ratio of bacterium and extreme thiobacillus ferrooxidans are 5:6:10:1:5.
Input amount of the mix bacterium agent in industrial wastewater sludge is 0.05%.
The application method of the mix bacterium agent, comprising the following steps:
(1) first time enrichment culture: taking the primary sludge of sewage treatment plant, and the Na of 5g/L is added into primary sludge2S2O3With
The FeSO of 4g/L4For substrate, mix bacterium agent is added, is passed through nitrogen and enrichment culture to sludge pH is down to 3.5-4.0, obtain first
Acclimation sludge after secondary enrichment culture；
(2) second of enrichment culture: the acclimation sludge after taking first time enrichment culture 1% is inoculated into raw dirt by volume
In mud, the Na of 3g/L is added2S2O3With the FeSO of 6g/L4For substrate, the enrichment culture under oxygen sufficiency, until pH value drops
Acclimation sludge to 2.0-2.5, after obtaining second of enrichment culture；Gained acclimation sludge mixed liquor is inoculum；
(3) bioleaching: using step (2) resulting inoculum, 0.5% be inoculated into primary sludge by volume, adds 5g/
The Na of L2S2O3With the FeSO of 4g/L4For substrate, initial pH value is adjusted to 5-7, leaching culture 16h, sulfur oxide sulphur at a temperature of 30 DEG C
Heavy metal separation in primary sludge is leached to water by the effects of bacillus and Thiobacillus ferrooxidans are restored by oxidation, acidification
Phase；
(4) sludge settling: the sludge settling after step (3) to be carried out to bioleaching obtains the sludge of low-heavy metal content
With the sewage of high content of beary metal.
The aeration intensity of the leaching culture of step (3) is controlled at 1.5L/ (minL)；The leaching culture of step (3) is stirred
Mixing revolving speed is 150r/min.
Embodiment 3:
It is a kind of for removing the mix bacterium agent of heavy metal in industrial wastewater sludge, including following component: Thiobacillus thioxidans,
Thiobacillus ferrooxidans, aerobic denitrifying bacteria, resistance to heavy metal bacterium and extreme thiobacillus ferrooxidans.
The resistance to heavy metal bacterium is P. aeruginosa bacterium disclosed in Chinese invention patent CN102337229A
ZGKD2。
The extreme thiobacillus ferrooxidans are that thiobacillus ferrooxidans disclosed in Chinese invention patent CN103131650A belong to ZJJN.
In the mix bacterium agent, Thiobacillus thioxidans, Thiobacillus ferrooxidans, aerobic denitrifying bacteria, resistance to heavy metal are thin
The weight ratio of bacterium and extreme thiobacillus ferrooxidans are 3:10:4:2:2.
Input amount of the mix bacterium agent in industrial wastewater sludge is 0.2%.
The application method of the mix bacterium agent, comprising the following steps:
(1) first time enrichment culture: taking the primary sludge of sewage treatment plant, and the Na of 3g/L is added into primary sludge2S2O3With
The FeSO of 6g/L4For substrate, mix bacterium agent is added, is passed through nitrogen and enrichment culture to sludge pH is down to 3.5-4.0, obtain first
Acclimation sludge after secondary enrichment culture；
(2) second of enrichment culture: the acclimation sludge after taking first time enrichment culture 0.5% is inoculated into life by volume
In sludge, the Na of 5g/L is added2S2O3With the FeSO of 4g/L4For substrate, the enrichment culture under oxygen sufficiency, until pH value
It is down to 2.0-2.5, the acclimation sludge after obtaining second of enrichment culture；Gained acclimation sludge mixed liquor is inoculum；
(3) bioleaching: using step (2) resulting inoculum, 1% be inoculated into primary sludge by volume, adds 3g/L
Na2S2O3With the FeSO of 6g/L4For substrate, initial pH value is adjusted to 5-7, at a temperature of 20 DEG C leaching culture for 24 hours, sulfur oxide sulphur
Heavy metal separation in primary sludge is leached to water by the effects of bacillus and Thiobacillus ferrooxidans are restored by oxidation, acidification
Phase；
(4) sludge settling: the sludge settling after step (3) to be carried out to bioleaching obtains the sludge of low-heavy metal content
With the sewage of high content of beary metal.
The aeration intensity of the leaching culture of step (3) is controlled at 1.0L/ (minL)；The leaching culture of step (3) is stirred
Mixing revolving speed is 250r/min.
Comparative example 1
By the aerobic denitrifying bacteria removal in embodiment 1, remaining proportion and methods for using them is constant.
Comparative example 2
By the removal of bacteria of resistance to heavy metal in embodiment 1, remaining proportion and methods for using them is constant.
Comparative example 3
By the extreme thiobacillus ferrooxidans removal in embodiment 1, remaining proportion and methods for using them is constant.
Detection is compared to the practical heavy metal processing capacity of the microbial inoculum sample of embodiment 1-3 and comparative example 1-4 below, is located
The content of each heavy metal species is as follows in sludge before reason: Cu element 95mgL-1, Cd element 45mgL-1, Hg element 18mg
L-1, Cr element 53mgL-1, Pd element 32mgL-1, the time unification of bioleaching is 18h and 96h, after statistical disposition
The removal rate of each heavy metal species, and compared with Chinese invention patent CN105776788A (reference examples), specific detection data
See Tables 1 and 2.
The removal rate of each heavy metal species in table 1:18h sludge；
The removal rate of each heavy metal species in table 2:96h sludge；
By the above test data it is recognised that aerobic denitrifying bacteria, resistance to heavy metal bacterium and extremely thermophilic are added in the present invention
Sour Thiobacillus is not only obviously improved the removal effect of the heavy metals such as Cu, Cd, Hg, Cr and Pd in sludge, and removal efficiency
It is obviously improved.
The foregoing is only a preferred embodiment of the present invention, but scope of protection of the present invention is not limited thereto,
Anyone skilled in the art in the technical scope disclosed by the present invention, according to the technique and scheme of the present invention and its
Inventive concept is subject to equivalent substitution or change, should be covered by the protection scope of the present invention.
Claims (6)
1. a kind of for removing the mix bacterium agent of heavy metal in industrial wastewater sludge, which is characterized in that including following component: oxidation
Sulphur Thiobacillus, Thiobacillus ferrooxidans, aerobic denitrifying bacteria, resistance to heavy metal bacterium and extreme thiobacillus ferrooxidans.
2. as described in claim 1 for removing the mix bacterium agent of heavy metal in industrial wastewater sludge, which is characterized in that described
Resistance to heavy metal bacterium be Chinese invention patent CN102337229A disclosed in P. aeruginosa bacterium ZGKD2.
3. as described in claim 1 for removing the mix bacterium agent of heavy metal in industrial wastewater sludge, which is characterized in that described
Extreme thiobacillus ferrooxidans be Chinese invention patent CN103131650A disclosed in thiobacillus ferrooxidans belong to ZJJN.
4. as described in claim 1 for removing the mix bacterium agent of heavy metal in industrial wastewater sludge, which is characterized in that described
Mix bacterium agent in, Thiobacillus thioxidans, Thiobacillus ferrooxidans, aerobic denitrifying bacteria, resistance to heavy metal bacterium and extreme acidophilus
The weight ratio of Thiobacillus is (3-5): (6-10): (4-10): (1-2): (2-5).
5. the mix bacterium agent for being used to remove heavy metal in industrial wastewater sludge as described in claim 1-4 is any, feature exist
In input amount of the mix bacterium agent in industrial wastewater sludge is 0.05-0.2%.
6. the mix bacterium agent for being used to remove heavy metal in industrial wastewater sludge as described in claim 1-4 is any, feature exist
In the application method of the mix bacterium agent, comprising the following steps:
(1) first time enrichment culture: taking the primary sludge of sewage treatment plant, and the Na of 3-5g/L is added into primary sludge2S2O3And 4-
The FeSO of 6g/L4For substrate, mix bacterium agent is added, is passed through nitrogen and enrichment culture to sludge pH is down to 3.5-4.0, obtain first
Acclimation sludge after secondary enrichment culture；
(2) second of enrichment culture: the acclimation sludge after taking first time enrichment culture, 0.5-1% is inoculated into raw dirt by volume
In mud, the Na of 3-5g/L is added2S2O3With the FeSO of 4-6g/L4For substrate, the enrichment culture under oxygen sufficiency, until pH
Value is down to 2.0-2.5, the acclimation sludge after obtaining second of enrichment culture；Gained acclimation sludge mixed liquor is inoculum；
(3) bioleaching: using step (2) resulting inoculum, and 0.5-1% is inoculated into primary sludge by volume, adds 3-5g/
The Na of L2S2O3With the FeSO of 4-6g/L4For substrate, initial pH value is adjusted to 5-7, leaching culture 16-24h at a temperature of 20-30 DEG C,
The effects of Thiobacillus thioxidans and Thiobacillus ferrooxidans are restored by oxidation, acidification, separates leaching for the heavy metal in primary sludge
Out to water phase；
(4) sludge settling: the sludge settling after step (3) to be carried out to bioleaching obtains the sludge and height of low-heavy metal content
The sewage of content of beary metal.
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| CN110723825A (en) * | 2019-11-21 | 2020-01-24 | 佛山科学技术学院 | Method for restoring black and odorous surface sewage |
| CN110745963A (en) * | 2019-11-21 | 2020-02-04 | 佛山科学技术学院 | Rapid bioremediation method for surface sewage |
| CN115090105A (en) * | 2022-07-12 | 2022-09-23 | 中泰莱(江苏)环境有限公司 | Fly ash recycling treatment method |
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| CN115090105A (en) * | 2022-07-12 | 2022-09-23 | 中泰莱(江苏)环境有限公司 | Fly ash recycling treatment method |
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| CN110129227B (en) | 2020-03-03 |
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