CN110117552A - One plant of Bei Laisi bacillus and its application - Google Patents

One plant of Bei Laisi bacillus and its application Download PDF

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Publication number
CN110117552A
CN110117552A CN201810110929.8A CN201810110929A CN110117552A CN 110117552 A CN110117552 A CN 110117552A CN 201810110929 A CN201810110929 A CN 201810110929A CN 110117552 A CN110117552 A CN 110117552A
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China
Prior art keywords
bacillus
bei laisi
potato
plant
bacterial strain
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CN201810110929.8A
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CN110117552B (en
Inventor
仲乃琴
宋双伟
赵盼
钞亚鹏
刘宁
汲恩民
郝长虹
赵广明
吴建强
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Hulunbuir Hengyi Agriculture And Animal Husbandry Co ltd
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Hulunbuir Hengyi Agriculture And Animal Husbandry Co ltd
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/07—Bacillus
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

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  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Virology (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Environmental Sciences (AREA)
  • Agronomy & Crop Science (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Pest Control & Pesticides (AREA)
  • Biomedical Technology (AREA)
  • Dentistry (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Plant Pathology (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The invention discloses one plant of Bei Laisi bacillus (Bacillus siamensis) bacterial strain HYNM-1, oneself is preserved in China General Microbiological center on October 12nd, 2017, and deposit number is CGMCC No.14804.The bacterial strain has inhibition of potato shot hole ability.

Description

One plant of Bei Laisi bacillus and its application
Technical field
The invention belongs to technical field of bioengineering more particularly to Bei Laisi bacillus (Bacillus Velezensis) and its be used to prepare prevention and treatment potato scab microbial inoculum in application.
Background technique
Potato scab, be it is a kind of endanger horse bell administration tuberous plant disease.It shows as stem tuber surface and subcircular occurs extremely The filbert scab of unsetting suberification scab shape or patch, it is coarse that hand touches texture.Two kinds of disease symptoms are generally divided into, are net respectively Line shape scab and breach shape scab.Although usual scab is only limitted to cortex, but killed potato wedge quality and yield can still reduce, intolerant to Storage, and sick potato appearance is indecency, commodity grade greatly declines, and incurs certain economic loss.In the past in China within the border due to hair Sick rate is not high, and area of causing harm is little, so not attracting people's attention.But in recent years, on Inner Mongol, Hebei, Shandong and other places The onset area in area is increasing, and caused economic loss is also considerable.Therefore, there is an urgent need to carry out science to this disease Prevention and treatment.
According to people for a long time to the separation identification research of pathogenic microorganism, it has been determined that cause the cause of disease of potato scab Bacterium is mainly scab streptomycete etc..And these pathogenic bacteria belong to conditioned pathogen, i.e., hold under the conditions of soil environment meta-alkalescence It easily grows and causes potato disease, and then seldom occur in Acid soil environment.
Control method mainly include the following types: (1), select disease-free potato seed；(2), more organic fertilizers or green manure can partially press down System morbidity；(3), crop rotations in 5 years or more are carried out with Curcurbitaceae, pulse family, liliaceous vegetable；(4) the vegetable plot plantation that selection has been retained, knot The potato phase meets arid and should water in time；(5), chemoprophylaxis: some chemical pesticides can be used to prevent and treat shot hole；(6) biological control Method: screening has good inhibitory effect and the strong microorganism of colonization ability to Bacterial Spot Pathogen, during producing potato seed and agriculture It is applied in the planting process of field, achievees the effect that inhibit growth of pathogenic bacteria.
Summary of the invention
The present invention provides the Bei Laisi bacillus (Bacillus that can effectively prevent potato scab Velezensis) bacterial strain HYNM-1.
Bei Laisi bacillus (Bacillus velezensis) bacterial strain HYNM-1 provided by the present invention, in 2017 It is deposited in China Committee for Culture Collection of Microorganisms's common micro-organisms center (referred to as are as follows: CGMCC), preservation on October 12, in Number are as follows: CGMCC No.14804, depositary institution address are as follows: Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3.
Detailed description of the invention:
Fungistatic effect figure of Fig. 1 Bei Laisi bacterial strain of bacillus HYNM-1 to shot hole streptomycete 4.1765.
Fungistatic effect figure of Fig. 2 Bei Laisi bacterial strain of bacillus HYNM-1 to shot hole streptomycete 4.1765.
Specific embodiment:
Strain acquires soil sample from Inner Mongol potato scab soil, and separation is identified and obtained.
Colonial morphology: cultivating 2 days on potato culture has protrusion among bacterium colony, and white, surface folding is irregularly unglazed Pool, edge are irregular；The shape of bacterial strain are as follows: bacterial strain is in the shape of a rod, has flagellum, and width is 0.76-0.94 μm, length 1.76- It is 2.94 μm, aerobic, produce gemma, Gram-positive.
Bacterium bacterial strain identification method: 16srRNA gene sequencing is identified as Bei Laisi bacillus (Bacillus velezensis)。
Method for cultivation of bacteria:
Bei Laisi bacillus activates 3 times in solid potato culture medium.Then 1 ring is taken to train in 50ml potato liquid Feeding base, 28 DEG C, shaking table culture 2 days.After fermentation liquid is centrifuged 1min clock through 12,000r/min, supernatant is collected separately, precipitating is suspended in In the sterile water of same volume, cell suspension is prepared.Bacterium solution is with supernatant also together for testing simultaneously.
Strain purposes and effect:
Shot hole Antagonistic Fungi screening material and method: test cause of disease is shot hole streptomycete (Streptomyces Scabiei) 4.1765. murphy juice culture medium (g/L): potato leaching liquid 1L, glucose 20g, KH2PO43g, MgSO4.7H2O 1.5g, vitamin B1 are micro.PH6. 15g agar is added in solid medium.
Bacteria culturing method: pathogen is cultivated 4-5 days, 28 DEG C on potato culture；It is scraped with 0.2ml sterile water Spore and mycelium are taken, certain density pathogen suspension is made；It is required that can be with after mycelia growth when 100ul volume spread plate It is paved with planar surface.
Bacteriostatic test method: 100ul pathogen is coated on plate (PDA), plate partial points connect 10ul bacterial solution, Supernatant and cell suspending liquid.28 DEG C of cultures.It is observed after 3 days.By to shot hole streptomycete (Streptomyces Scabiei) 4.1765 test finds that bacterial solution and cell suspending liquid can effectively inhibit these pathogenic microorganisms to grow, on There is no inhibitory effect clearly.
As a result: bacterium is effective to pathogen (Fig. 1, Fig. 2).

Claims (2)

1. one plant of Bei Laisi bacillus (Bacillus siamensis) bacterial strain HYNM-1, deposit number CGMCC No.14804。
2. Bei Laisi bacillus (Bacillus siamensis) bacterial strain HYNM-1 described in claim 1 inhibits horse in preparation Application in bell potato scab medicine.
CN201810110929.8A 2018-02-05 2018-02-05 Bacillus belgii and application thereof Active CN110117552B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201810110929.8A CN110117552B (en) 2018-02-05 2018-02-05 Bacillus belgii and application thereof

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201810110929.8A CN110117552B (en) 2018-02-05 2018-02-05 Bacillus belgii and application thereof

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CN110117552A true CN110117552A (en) 2019-08-13
CN110117552B CN110117552B (en) 2022-07-08

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111908964A (en) * 2020-05-14 2020-11-10 中国科学院微生物研究所 Stable synergistic fertilizer additive and preparation method thereof

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU1461097A (en) * 1996-11-29 1998-06-22 Micro Flo Company Agricultural compositions containing bacteria
CN107236693A (en) * 2017-07-04 2017-10-10 中国农业科学院农产品加工研究所 Bei Laisi bacillus JS25R and its application

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU1461097A (en) * 1996-11-29 1998-06-22 Micro Flo Company Agricultural compositions containing bacteria
CN107236693A (en) * 2017-07-04 2017-10-10 中国农业科学院农产品加工研究所 Bei Laisi bacillus JS25R and its application

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
JUAN M.PALAZZINI等人: "Bacillus velezensis RC 218 as a biocontrol agent to reduce Fusarium head blight and deoxynivalenol accumulation: Genome sequencing and secondary metabolite cluster profiles", 《MICROBIOLOGICAL RESEARCH》 *
高同国等: "马铃薯疮痂病高效拮抗菌的筛选及鉴定", 《江苏农业科学》 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111908964A (en) * 2020-05-14 2020-11-10 中国科学院微生物研究所 Stable synergistic fertilizer additive and preparation method thereof

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