CN110106571A - A kind of spider web textile fabric and preparation method thereof - Google Patents
A kind of spider web textile fabric and preparation method thereof Download PDFInfo
- Publication number
- CN110106571A CN110106571A CN201910282080.7A CN201910282080A CN110106571A CN 110106571 A CN110106571 A CN 110106571A CN 201910282080 A CN201910282080 A CN 201910282080A CN 110106571 A CN110106571 A CN 110106571A
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- China
- Prior art keywords
- spider
- thread
- textile fabric
- protein
- preparation
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
-
- D—TEXTILES; PAPER
- D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
- D01F—CHEMICAL FEATURES IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS; APPARATUS SPECIALLY ADAPTED FOR THE MANUFACTURE OF CARBON FILAMENTS
- D01F4/00—Monocomponent artificial filaments or the like of proteins; Manufacture thereof
-
- D—TEXTILES; PAPER
- D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
- D01F—CHEMICAL FEATURES IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS; APPARATUS SPECIALLY ADAPTED FOR THE MANUFACTURE OF CARBON FILAMENTS
- D01F8/00—Conjugated, i.e. bi- or multicomponent, artificial filaments or the like; Manufacture thereof
- D01F8/02—Conjugated, i.e. bi- or multicomponent, artificial filaments or the like; Manufacture thereof from cellulose, cellulose derivatives, or proteins
Abstract
The invention discloses a kind of spider web textile fabrics and preparation method thereof, belong to field of textile, a kind of spider web textile fabric, the textile fabric is by extracting and replicating spider's thread protein, spider's thread protein is separated, artificial spider's thread progress textile process is made, to form textile fabric, a kind of preparation method of spider web textile fabric, the following steps are included: S1: extracting the spider's thread, S2: duplication spider's thread protein, S3: bionical, the S4: the preparation of textile fabric of spinning process, the artificial spider's thread come will be copied and carry out textile process.Preparation method of the invention secrets out of spider's thread protein similar with spider tension cable wire, 80% the above are protein similar with spider's thread protein in the non-soluble composition of Yeast protein after breeding, and yield is considerable, the separation and purifying of protein are also carried out, to carry out spinning later, while ensure that high molecular weight output, the yield and uniformity of spider web textile fabric are all improved.
Description
Technical field
The present invention relates to field of textile, more specifically to a kind of preparation method of spider web textile fabric.
Background technique
The spider's thread is made of some fiber ropes for being referred to as fibril, and the spider's thread has very high intensity, and elastic and flexible
Property it is all fine, impact resistance is strong, and resistance to low temperature is good, be still able to maintain under conditions of -40 DEG C its elasticity, needing low temperature to use
Occasion, advantage is particularly significant, and the spider's thread is made of protein, thus be it is biodegradable, dirt will not be caused to environment
Dye, thus be widely used in military affairs, aerospace, building and reason material etc. industries, the spider's thread due to obtain difficulty, while amount to obtain compared with
Small, then people carry out the production of the artificial spider's thread by gene engineering method, and the existing artificial spider's thread mostly uses animals and plants to produce,
Yield and uniformity are all restricted.
Summary of the invention
1. technical problems to be solved
Aiming at the problems existing in the prior art, the purpose of the present invention is to provide a kind of spider web textile fabric and its preparations
Method, it may be implemented to secret out of spider's thread protein similar with spider tension cable wire, the insoluble group of Yeast protein after breeding
80% the above are protein similar with spider's thread protein in part, and yield is considerable, has also carried out separation and purifying of protein etc.,
To carry out spinning later, while ensure that high molecular weight output, yield and uniformity are all improved.
2. technical solution
To solve the above problems, the present invention adopts the following technical scheme that.
A kind of spider web textile fabric, the textile fabric are separated spider's thread protein by extracting and replicating spider's thread protein
The artificial spider's thread is made and carries out textile process, to form textile fabric.
A kind of preparation method of spider web textile fabric, comprising the following steps:
S1: the spider's thread is extracted, its albumen and glandular secretion object are researched and analysed;
S2: duplication spider's thread protein;
S3: spider's thread protein sub-wire;
S4: spinning process it is bionical so that the spider's thread be not dissolved in water;
S5: the preparation of textile fabric.
Further, in S1, the nucleotide sequence of research spider's thread protein gene coding, while carrying out point of gene order
From, purifying, structure feature expression.
Further, in S2, the gene order model of different spider's thread protein segments is established on the basis of S1, use is this
Model can produce the synthesis gene of referred to as spider's thread protein, and then by this synthesis gene transplant to microorganism, microorganism is a large amount of
This spider's thread protein is replicated, this spider's thread protein is separated.
Further, microorganism uses saccharomycete.
Further, in S4, the spider's thread after duplication is purified, is dissolved and spinning, it is made to be no longer dissolve in water.
Further, in S5, the artificial spider's thread come will be copied and carry out textile process, to form textile fabric.
Further, the elongation of the spider's thread is 9.8%-32.1%, and intensity is 1 × 10^9N/ ㎡, and work to break is 1 × 10^
5/㎏。
Further, the protein molecule length of the spider's thread is up to 1000 amino acid.
3. beneficial effect
Compared with the prior art, the present invention has the advantages that
1, saccharomycete can secret out of spider's thread protein similar with spider tension cable wire, and Yeast protein is insoluble after breeding
80% the above are protein similar with spider's thread protein in component, and yield is considerable, has also carried out the separation and purifying of protein
Deng to carry out spinning later, while ensure that high molecular weight output, yield and uniformity are all improved.
2, sub-wire is carried out to spider's thread protein, spider's thread protein is divided into filiform, and the spider's thread and fiber are mixed to form weaving fibre
Silk is tieed up, spider's thread cost is reduced, while the spider's thread is formed well and is weaved convenient for fabric.
Detailed description of the invention
Fig. 1 is overall flow figure of the invention.
Specific embodiment
Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, complete
Site preparation description;Obviously, described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments, is based on
Embodiment in the present invention, it is obtained by those of ordinary skill in the art without making creative efforts every other
Embodiment shall fall within the protection scope of the present invention.
In the description of the present invention, it should be noted that the instruction such as term " on ", "lower", "inner", "outside", " top/bottom end "
Orientation or positional relationship be based on the orientation or positional relationship shown in the drawings, be merely for convenience of description the present invention and simplification retouch
It states, rather than the device or element of indication or suggestion meaning must have a particular orientation, be constructed and operated in a specific orientation,
Therefore it is not considered as limiting the invention.In addition, term " first ", " second " are used for description purposes only, and cannot understand
For indication or suggestion relative importance.
In the description of the present invention, it should be noted that unless otherwise clearly defined and limited, term " installation " " is set
Be equipped with ", " be arranged/connect ", " connection " etc., shall be understood in a broad sense, such as " connection ", may be a fixed connection, be also possible to removable
Connection is unloaded, or is integrally connected;It can be mechanical connection, be also possible to be electrically connected;It can be directly connected, it can also be in
Between medium be indirectly connected, can be the connection inside two elements.It for the ordinary skill in the art, can be specific
Situation understands the concrete meaning of above-mentioned term in the present invention.
Embodiment 1:
Please refer to Fig. 1
A kind of spider web textile fabric, the textile fabric are separated spider's thread protein by extracting and replicating spider's thread protein
The artificial spider's thread is made and carries out textile process, to form textile fabric.
A kind of preparation method of spider web textile fabric, comprising the following steps:
S1: extracting the spider's thread, research and analyse to its albumen and glandular secretion object, the core of research spider's thread protein gene coding
Nucleotide sequence, while carrying out the separation, purifying, the expression of structure feature of gene order;
S2: duplication spider's thread protein establishes the gene order mould of different spider's thread protein segments in S2 on the basis of S1
Type can produce the synthesis gene of referred to as spider's thread protein with this model, micro- then by this synthesis gene transplant to microorganism
Biological this spider's thread protein of massive duplication, this spider's thread protein is separated, and saccharomycete can secret out of and spider tension cable wire phase
As spider's thread protein, 80% the above are albumen similar with spider's thread protein in the non-soluble composition of Yeast protein after breeding
Matter, and yield is considerable, has also carried out separation and purifying of protein etc., to carry out spinning later, ensure that macromolecule volume production
While out, yield and uniformity are all improved;
S3: spider's thread protein sub-wire carries out sub-wire to spider's thread protein, spider's thread protein is divided into filiform, and by the spider's thread and fiber
It is mixed to form textile fabric silk, reduces spider's thread cost, while the spider's thread is formed well and is weaved convenient for fabric;
S4: spinning process it is bionical so that the spider's thread be not dissolved in water, the spider's thread after duplication is purified, dissolved and is spun
Silk, makes it be no longer dissolve in water;
S5: the preparation of textile fabric will copy the artificial spider's thread come and carry out textile process, to form spinning in S4
Knitted fabric.
The spider web textile fabric and preparation method thereof can secret out of spider's thread egg similar with spider tension cable wire by saccharomycete
White, 80% the above are protein similar with spider's thread protein in the non-soluble composition of Yeast protein after breeding, and yield can
It sees, has also carried out separation and purifying of protein etc., to carry out spinning later, while ensure that high molecular weight output, produce
Amount and uniformity are all improved.
The foregoing is intended to be a preferred embodiment of the present invention;But scope of protection of the present invention is not limited thereto.
Anyone skilled in the art in the technical scope disclosed by the present invention, according to the technique and scheme of the present invention and its
It improves design and is subject to equivalent substitution or change, should be covered by the scope of protection of the present invention.
Claims (9)
1. a kind of spider web textile fabric, it is characterised in that: the textile fabric is by extracting and replicating spider's thread protein, by spider's thread protein
It separates and artificial spider's thread progress textile process is made, to form textile fabric.
2. a kind of preparation method of spider web textile fabric, which comprises the following steps:
S1: the spider's thread is extracted, its albumen and glandular secretion object are researched and analysed;
S2: duplication spider's thread protein;
S3: spider's thread protein sub-wire;
S4: spinning process it is bionical so that the spider's thread be not dissolved in water;
S5: the preparation of textile fabric.
3. a kind of spider web textile fabric according to claim 2 and preparation method thereof, it is characterised in that: in the S1,
The nucleotide sequence of spider's thread protein gene coding is studied, while carrying out the separation, purifying, the expression of structure feature of gene order.
4. a kind of spider web textile fabric according to claim 2 and preparation method thereof, it is characterised in that: in the S2,
The gene order model that different spider's thread protein segments are established on the basis of S1 can produce referred to as spider's thread protein with this model
Synthesis gene, then by this synthesis gene transplant to microorganism, this spider's thread protein of microorganism massive duplication, by this spider
Silk-fibroin is separated.
5. a kind of spider web textile fabric according to claim 4 and preparation method thereof, it is characterised in that: the microorganism is adopted
Use saccharomycete.
6. a kind of spider web textile fabric according to claim 2 and preparation method thereof, it is characterised in that: in the S4,
The spider's thread after duplication is purified, is dissolved and spinning, it is made to be no longer dissolve in water.
7. a kind of spider web textile fabric according to claim 2 and preparation method thereof, it is characterised in that: in the S5,
The artificial spider's thread come will be copied and carry out textile process, to form textile fabric.
8. a kind of spider web textile fabric according to claim 2 and preparation method thereof, it is characterised in that: the spider's thread is stretched
Long rate is 9.8%-32.1%, and intensity is 1 × 10^9N/ ㎡, and work to break is 1 × 10^5/ ㎏.
9. a kind of spider web textile fabric according to claim 2 and preparation method thereof, it is characterised in that:
The protein molecule length of the spider's thread is up to 1000 amino acid.
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CN201910282080.7A CN110106571A (en) | 2019-04-09 | 2019-04-09 | A kind of spider web textile fabric and preparation method thereof |
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CN201910282080.7A CN110106571A (en) | 2019-04-09 | 2019-04-09 | A kind of spider web textile fabric and preparation method thereof |
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Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1200145A (en) * | 1995-08-22 | 1998-11-25 | 阿格里科拉技术有限公司 | Cloning methods for high strength spider silk proteins |
CN1362520A (en) * | 2001-01-02 | 2002-08-07 | 成都天友发展有限公司 | Constitution process of blank expression system for transferring spider's dragline protein gene into Bombyx mori |
CN102906107A (en) * | 2010-03-11 | 2013-01-30 | 韩国科学技术院 | High molecular weight recombinant silk or silk-like protein, and micro- or nanoscale spider-web fiber or spider-web-like fiber produced using the recombinant silk or silk-like protein |
CN107699567A (en) * | 2017-10-27 | 2018-02-16 | 东华大学 | A kind of Araneus ventricosus parcel silk-fibroin full-length gene and preparation method thereof |
-
2019
- 2019-04-09 CN CN201910282080.7A patent/CN110106571A/en not_active Withdrawn
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1200145A (en) * | 1995-08-22 | 1998-11-25 | 阿格里科拉技术有限公司 | Cloning methods for high strength spider silk proteins |
CN1362520A (en) * | 2001-01-02 | 2002-08-07 | 成都天友发展有限公司 | Constitution process of blank expression system for transferring spider's dragline protein gene into Bombyx mori |
CN102906107A (en) * | 2010-03-11 | 2013-01-30 | 韩国科学技术院 | High molecular weight recombinant silk or silk-like protein, and micro- or nanoscale spider-web fiber or spider-web-like fiber produced using the recombinant silk or silk-like protein |
CN107699567A (en) * | 2017-10-27 | 2018-02-16 | 东华大学 | A kind of Araneus ventricosus parcel silk-fibroin full-length gene and preparation method thereof |
Non-Patent Citations (1)
Title |
---|
杨子江等: "大腹园蛛次壶腹腺丝的表达 ", 《生物工程学报》 * |
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