CN110104768A - A kind of cultural method for the denitrification sludge being resistant to nano material - Google Patents
A kind of cultural method for the denitrification sludge being resistant to nano material Download PDFInfo
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- CN110104768A CN110104768A CN201910314903.XA CN201910314903A CN110104768A CN 110104768 A CN110104768 A CN 110104768A CN 201910314903 A CN201910314903 A CN 201910314903A CN 110104768 A CN110104768 A CN 110104768A
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/28—Anaerobic digestion processes
- C02F3/2846—Anaerobic digestion processes using upflow anaerobic sludge blanket [UASB] reactors
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- C—CHEMISTRY; METALLURGY
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- C02F2203/00—Apparatus and plants for the biological treatment of water, waste water or sewage
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Abstract
The invention discloses a kind of cultural methods of denitrification sludge for being resistant to nano material, comprising: denitrification sludge is inoculated in reactor, being pumped into pH is 7.2~7.5 containing NO3 ‑- N water inlet is run 10~15 days under conditions of being protected from light, temperature is 30~36 DEG C, hydraulic detention time is constant；Gradient reduces reactor hydraulic detention time, and each gradient is run 10~30 days, forms denitrification granular sludge, and the denitrification granular sludge activity recorded at this time is control group activity；Nano material is added into water inlet, and gradient improves nano material concentration in water inlet, each gradient is run 10~15 days, when running active for control group to denitrification granular sludge activity 50%~60%, stops improving nano material concentration in water inlet；Phosphate is added into water inlet, phosphate concn is 10~15mmol/L in water inlet, completes culture.The granule sludge tolerance that the present invention is cultivated is remarkably reinforced, and extracellular polymeric content is high, can resist the infringement of other poisonous substances, and load is high, nitrogen removal performance is stablized.
Description
Technical field
The present invention relates to culturing sludge technical fields, and in particular to a kind of culture for the denitrification sludge for being resistant to nano material
Method.
Background technique
The noxious material for causing sludge to be poisoned refer to the microbial physiology inhibited certain inanimate matters of activity and
Organic matter mainly has the nonmetallic compounds such as the heavy metal ion such as zinc, copper, nickel, lead, chromium and phenol, aldehyde, cyanide, sulfide.Have
Toxic action of the noxious material to microorganism has the concept of an amount, only when noxious material reaches a certain concentration in the environment,
It poisons and inhibiting effect just displays.As long as the various noxious materials in sewage are lower than this concentration, the physiology function of microorganism
It can be unaffected.Noxious material effect also with pH value, water temperature, dissolved oxygen, whether there is or not the quantity of other noxious materials and microorganism
And it is whether related by factors such as domestications.
With the high demand of sewage water denitrification, biological denitrification process is widely used to sewage treatment.Denitrification is de- in biology
Important role is played in nitrogen technique, is able to maintain to stablize and is realized best biological denitrificaion efficiency.Anaerobic sludge has the ability to be formed
Self-retaining particle provides advantageous physics and electrochemical conditions for the flocculation of sludge.
Previous studies also indicate that denitrifying bacteria is capable of forming the granule sludge well precipitated.Granule sludge is than cotton-shaped
Sludge has more advantages, such as better settling property, fine and close structure and bigger shock resistance, is conducive to biomass guarantor
It stays, it is ensured that the operation of reactor efficient stable.
From twentieth century end, gradually development has been a new scientific domain to nanotechnology so far.However, fast-developing
Nanotechnology already leads to many industrial circles and has a revolutionary change.It is answered in the manufacture of new consumer products and much industry
In, the engineering risk of nanoparticle (NPs) has caused the extensive concern of government, research institution and the public.Due to small ruler
Very little effect, quantum effect and bigger serface and other specific physicals and chemical property, the phase between biology and nano particle
Interaction is complex.
CuNPs has been widely used in work relevant to rubber, smelting, power station and fuel cell.Nano particle exists
Release in production process, using or disposition the product containing nano particle be widely recognized as.For example, semiconductor, health and change
The increase in demand of cosmetic can produce a large amount of waste water containing NPs.NPs can also by bathing, laundry, cleaning and rainwater from the consumer goods to
Sewage treatment plant's release.Finally, nano particle is present in waste water, sewage sludge and percolate from garbage filling field.
Currently, influence of the NPs to activated Sludge System has become international research hot spot.Research NPs environment return, environment
Behavior and environment influence to have a very important significance to carrying out a nanometer contamination control in a deep going way.Therefore, NPs is to biological treatment work
Skill proposes new challenge.
Summary of the invention
For shortcoming existing for this field, the present invention provides a kind of trainings of denitrification sludge for being resistant to nano material
The method of supporting, improves denitrification granular sludge technique to the tolerance of highly concentrated nano material, the granule sludge tolerance cultivated
Property be remarkably reinforced, extracellular polymeric content is high, can resist the infringement of other poisonous substances, and load is high, and nitrogen removal performance is stablized.
A kind of cultural method for the denitrification sludge being resistant to nano material, comprising:
(1) it is inoculated with denitrification sludge in reactor, is pumped into containing NO3 -The water inlet of-N, inlet flow-patterm are 7.2~7.5, are being kept away
It is run 10~15 days under conditions of light, temperature are 30~36 DEG C, hydraulic detention time is constant；
(2) gradient reduces reactor hydraulic detention time, and each gradient is run 10~30 days, and denitrification sludge accumulation is formed
Denitrification granular sludge, the denitrification granular sludge activity recorded at this time is control group activity；
(3) it is added nano material into water inlet, and gradient improves nano material concentration in water inlet, each gradient operation 10~
15 days, when running active for control group to denitrification granular sludge activity 50%~60%, stop improving nanometer material in water inlet
Expect concentration；
(4) phosphate is added into water inlet, phosphate concn is 10~15mmol/L in water inlet, and it is dirty to complete denitrification granular
Tolerance culture of the mud to nano material.
The present invention is stepped up first into nano material load in water, and operation closes on collapse to reactor performance, then adds
Certain density phosphate is added to alleviate the toxicity of nano material, it is operation to the activity of denitrification granular sludge, dehydrogenase, extracellular poly-
Conjunction object is close with seed sludge or is higher than seed sludge, to improve denitrification granular sludge technique to highly concentrated nano material
Tolerance, the granule sludge tolerance cultivated are remarkably reinforced, and extracellular polymeric content is high, can resist the infringement of other poisonous substances, and
Load is high, and nitrogen removal performance is stablized.
In step (1), it is preferable that the concentration of the denitrification sludge is 10~15g VSS L-1, it is advantageously implemented higher
Load.
The dominant bacteria of the denitrification sludge is Castellaniella.
Preferably, in step (1), the hydraulic detention time is 4~5h, is advantageously implemented higher denitrifying load.
Preferably, NO in water inlet3 -- N concentration is not less than 600mg/L, is advantageously implemented higher denitrifying load, simulates high nitric acid
Salt waste water.
In step (2), it is preferable that the hydraulic detention time of each gradient is the 1/2 of the hydraulic detention time of a upper gradient
~2/3.
In step (2), the partial size of the denitrification granular sludge of formation is 0.01~3mm, has preferable impact resistance.
In step (3), the nano material is Nanometer Copper and/or nano zine oxide, is widely used, toxicity height.
The partial size of the Nanometer Copper is 10~30nm, and the nanometer copper product of the partial size is using more, and partial size is small, and toxicity is high.
The partial size of the nano zine oxide is 20~50nm, and using more, partial size is small for the nano zinc oxide material of the partial size,
Toxicity is high.
In step (3), it is preferable that the concentration of nano material is added for the first time into water inlet no more than 2mg L-1, concentration from
The low to high reactor that facilitates generates tolerance.
Preferably, in step (3), nano material concentration is 1.5~3 times of a upper gradient in each gradient water inlet.
In step (4), phosphate main function is to reduce metal ion in conjunction with the metal ion of nano material release
Biodegradability, to reduce the toxicity of nano material.
Preferably, the phosphate is disodium hydrogen phosphate and/or sodium dihydrogen phosphate.Disodium hydrogen phosphate and/or di(2-ethylhexyl)phosphate
Hydrogen sodium is the constituent of phosphate buffer, will not generate biggish negative effect to system.
Compared with prior art, the present invention major advantage includes: the tolerable height of denitrification granular sludge that the present invention obtains
The nano material of concentration, and activity and extracellular polymeric content are higher, it can be achieved that high load capacity denitrogenation.
Specific embodiment
Present invention will be further explained below with reference to specific examples.It should be understood that these embodiments are merely to illustrate the present invention
Rather than it limits the scope of the invention.In the following examples, the experimental methods for specific conditions are not specified, usually according to conventional strip
Part, or according to the normal condition proposed by manufacturer.
(1) upflow sludge bed reactor for using effective volume 1.0L, is inoculated with denitrification sludge, and reactor is placed in 35 ±
In 1 DEG C of thermostatic chamber, it is protected from light operation, initial sludge concentration 13.0g VSS L-1, hydraulic detention time 4.8h, operation to stabilization.
Reactor, which is initially intake into, to be grouped as are as follows:
Wherein, the microelement I stock solution composition are as follows:
EDTA 5.00g L-1,
FeSO4 9.14g L-1。
Microelement II stock solution composition are as follows:
(2) gradient reduces reactor hydraulic detention time, and domestication denitrification sludge accumulation forms denitrification granular sludge.
Hydraulic detention time is reduced to 3.2h, is run 15 days, denitrification sludge partial size does not have significant changes.
Continue to reduce hydraulic detention time to 1.92h, after operation 15 days, denitrification granular sludge is gradually formed, and partial size is
1.46±1.42mm。
Monitoring hydraulic detention time is 1.92h, the water outlet total nitrogen and denitrification granular sludge of the reactor after operation 15 days
Activity and extracellular polymeric content, be labeled as control group；Denitrification activity is 504.5 ± 44.3mg TN g-1VSS d-1, born of the same parents
Outer polymer content is 145.7 ± 8.0mg g-1VSS；Identified strain is Castellaniella.
(3) load into nano material in water is stepped up, runs to the performance of reactor and closes on collapse.
1mg L is added in water inlet-1Nanometer Copper, after carrying out culture in 15 days by a definite date, denitrification activity be 457.5 ±
13.8mg TN g-1VSS d-1, extracellular polymeric content is 139.1 ± 2.2mg g-1VSS, reactor are discharged total nitrogen and are lower than 10mg
L-1。
The concentration of Nanometer Copper is mentioned to 2mg L in water inlet-1, after continuing culture 15 days, denitrification activity is 440.3 ± 41.8mg
TN g-1VSS d-1, extracellular polymeric content is 139.5 ± 4.4mg g-1VSS, reactor are discharged total nitrogen and are lower than 10mg L-1。
Water inlet nanometer copper concentration is improved to 5mg L-1After continue culture 30 days, compared with the control group, denitrification activity is obvious
44% is reduced, is 282.5 ± 14.7mg TN g-1VSS d-1, extracellular polymeric content reduces 15%, be 123.9 ±
5.2mg g-1VSS, reactor water outlet total nitrogen are the 55.3% of water inlet total nitrogen, are 332.8mg L-1, reactor performance obviously dislikes
Change.
(4) sodium dihydrogen phosphate is then added in water inlet, phosphate dihydrogen sodium concentration is 10mmol L in water inlet-1, culture 15
After it, reactor is discharged total nitrogen and is lower than 10mg L-1, nitrogen removal efficiency reaches 7.8kg N m-3d-1, nitrogen removal efficiency reaches
98.3%, denitrification activity reverts to the 95.7% of control group, and extracellular polymeric content is close with control group, shows phosphatic
Addition effectively alleviates the toxicity of nano material.So far, tolerance culture of the denitrification granular sludge to nano material is completed.
In general, nano material is alleviated to denitrification sludge toxicity, to enhance denitrification by addition phosphate
Tolerance of the grain sludge to nano material.
When Nanometer Copper significantly inhibits denitrification granular sludge system, phosphatic addition significantly reduces Nanometer Copper release
Copper ion biodegradability, to alleviate the toxicity of Nanometer Copper, the sludge extracellular polymeric content cultivated is high, favorably
In the defense function of enhancing granule sludge, and load is high, and nitrogen removal performance is stablized.
In addition, it should also be understood that, those skilled in the art can be to this hair after having read foregoing description content of the invention
Bright to make various changes or modifications, these equivalent forms also fall within the scope of the appended claims of the present application.
Claims (10)
1. a kind of cultural method for the denitrification sludge for being resistant to nano material, comprising:
(1) it is inoculated with denitrification sludge in reactor, is pumped into containing NO3 -The water inlet of-N, inlet flow-patterm be 7.2~7.5, be protected from light, temperature
It is run 10~15 days under conditions of degree is 30~36 DEG C, hydraulic detention time is constant；
(2) gradient reduces reactor hydraulic detention time, and each gradient is run 10~30 days, and denitrification sludge accumulation forms anti-nitre
Change granule sludge, the denitrification granular sludge activity recorded at this time is control group activity；
(3) nano material is added into water inlet, and gradient improves nano material concentration in water inlet, each gradient operation 10~15
It, when running active for control group to denitrification granular sludge activity 50%~60%, stops improving nano material in water inlet
Concentration；
(4) phosphate is added into water inlet, phosphate concn is 10~15mmol/L in water inlet, completes denitrification granular sludge pair
The tolerance culture of nano material.
2. the cultural method of the denitrification sludge of tolerance nano material according to claim 1, which is characterized in that described anti-
The concentration of nitrifying sludge is 10~15g VSS L-1。
3. the cultural method of the denitrification sludge of tolerance nano material according to claim 1, which is characterized in that described anti-
The dominant bacteria of nitrifying sludge is Castellaniella.
4. the cultural method of the denitrification sludge of tolerance nano material according to claim 1, which is characterized in that step
(2) in, the hydraulic detention time of each gradient is the 1/2~2/3 of the hydraulic detention time of a upper gradient.
5. the cultural method of the denitrification sludge of tolerance nano material according to claim 1, which is characterized in that step
(2) in, the partial size of the denitrification granular sludge of formation is 0.01~3mm.
6. the cultural method of the denitrification sludge of tolerance nano material according to claim 1, which is characterized in that described
Nano material is Nanometer Copper and/or nano zine oxide.
7. the cultural method of the denitrification sludge of tolerance nano material according to claim 6, which is characterized in that described to receive
The partial size of rice copper is 10~30nm；
The partial size of the nano zine oxide is 20~50nm.
8. the cultural method of the denitrification sludge of tolerance nano material according to claim 1, which is characterized in that step
(3) in, the concentration of nano material is added for the first time into water inlet no more than 2mgL-1。
9. the cultural method of the denitrification sludge of tolerance nano material according to claim 1 or 8, which is characterized in that step
Suddenly in (3), nano material concentration is 1.5~3 times of a upper gradient in each gradient water inlet.
10. the cultural method of the denitrification sludge of tolerance nano material according to claim 1, which is characterized in that described
Phosphate be disodium hydrogen phosphate and/or sodium dihydrogen phosphate.
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| CN113308400A (en) * | 2021-05-24 | 2021-08-27 | 上海交通大学 | OFA38 strain of Castellaniella sp, and screening method and application thereof |
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