CN108264144A - A kind of cultural method for the denitrification granular sludge for being resistant to sulfamethoxazole - Google Patents
A kind of cultural method for the denitrification granular sludge for being resistant to sulfamethoxazole Download PDFInfo
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- CN108264144A CN108264144A CN201711490120.4A CN201711490120A CN108264144A CN 108264144 A CN108264144 A CN 108264144A CN 201711490120 A CN201711490120 A CN 201711490120A CN 108264144 A CN108264144 A CN 108264144A
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/28—Anaerobic digestion processes
- C02F3/2846—Anaerobic digestion processes using upflow anaerobic sludge blanket [UASB] reactors
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2305/00—Use of specific compounds during water treatment
- C02F2305/06—Nutrients for stimulating the growth of microorganisms
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Abstract
The invention discloses a kind of cultural methods for the denitrification granular sludge for being resistant to sulfamethoxazole to use up flow anaerobic sludge blanket reactor, stable using simulated wastewater as water inlet using denitrification granular sludge as seed sludge, and 0.1mgL is added into water inlet‑1Sulfamethoxazole, when running to 70~80% that sludge activity in reactor is seed sludge activity, sulfamethoxazole is added into reactor water inlet by mode incremental stage by stage again, it is run 13~15 days under each concentration, when denitrification sludge activity reaches more than the 70% of seed sludge activity in reactor, and when sludge activity no longer reduces for 13~15 days in reactor, the denitrification granular sludge of tolerance sulfamethoxazole is obtained.The present invention is resistant to sulfamethoxazole 10mgL by the method culture that gradient is tamed‑1Denitrification granular sludge, it is ensured that denitrification process stable operation.
Description
(1) technical field
The present invention relates to a kind of cultural methods of denitrification granular sludge, more particularly to a kind of to be resistant to the anti-of sulfamethoxazole
The cultural method of nitrifying granular activated sludge.
(2) background technology
In recent years, how economically and efficiently to handle cultivation factory waste water becomes the content that researcher probes into.Denitrification process by
In can be achieved at the same time the removal of organic matter and nitrogenous compound by people's extensive use.However, in cultivation factory waste water, there are one
A little substances for inhibiting denitrifying bacterium, sulfamethoxazole are exactly one of them.Research shows that sulfamethoxazole can prevent peptide chain
Increase the synthesis with protein, the growth so as to inhibit bacteria.Based on this, if the denitrification of tolerance sulfamethoxazole can be turned out
Sludge is then expected to break through the technical bottleneck of cultivation factory wastewater treatment.The present invention constructs the denitrification of tolerance sulfamethoxazole
A kind of cultural method of grain sludge, tames denitrification granular sludge using sulfamethoxazole concentration gradient method for improving,
Improve tolerance of the denitrification granular sludge to sulfamethoxazole.
(3) invention content
It is an object of the present invention to provide a kind of cultural methods for the denitrification granular sludge for being resistant to sulfamethoxazole.
The technical scheme is that：
The present invention discloses a kind of cultural method for the denitrification granular sludge for being resistant to sulfamethoxazole, and the method is：It adopts
With up flow anaerobic sludge blanket reactor, using denitrification granular sludge as seed sludge, to contain nitrate nitrogen, organic carbon, remain micro-
The simulated wastewater of inorganic salt buffer needed for biological growth and trace element is water inlet, in anaerobism, temperature be 35 ± 1 DEG C, into
Under conditions of water pH is 7.2~7.4, hydraulic detention time is 2~3h, 0.1mgL is added into water inlet-1Sulfamethoxazole, fortune
Row to sludge activity in reactor for seed sludge activity 70~80% when, then by mode incremental stage by stage to reactor
Sulfamethoxazole is added in water inlet, is run 13~15 days under each concentration, when denitrification sludge activity reaches inoculation in reactor
More than 70% sludge activity, and when sludge activity no longer reduces for 13~15 days in reactor, obtain tolerance sulfamethoxazole
Denitrification granular sludge.
Further, a concentration of 0.1~10mg L of sulfamethoxazole in the water inlet-1, the sulfamethoxazole addition concentration
It is 2~5 times of concentration on last stage, is run 13~15 days under each concentration.
Further, the sulfamethoxazole adding method is：0.1mgL is added into water inlet for the first time-1Sulfalene dislike
Azoles runs to denitrification sludge activity in reactor and reaches the 70~80% of seed sludge activity, then adds respectively into water inlet
Add 0.2mgL-1、0.4mg·L-1、1mg·L-1、2
mg·L-1、10mg·L-1Sulfamethoxazole, run 13~15 days under each concentration, sludge activity in reactor
It remains unchanged for the 80% of initial sludge activity and 15 days, obtains the denitrification granular sludge of tolerance sulfamethoxazole.
Further, the activity of the seed sludge is 420~680mg Ng-1VSS·d-1, grain size is 1.8~3.6mm,
It is 40~60gL respectively to be inoculated with suspended sediment concentration and volatile suspended solids concentration in post-reactor-1With 30~40gL-1。
Further, in the simulated wastewater origin of organic carbon in one kind of glucose, sodium acetate, methanol or molasses or more
Kind.
Further, NO in the water inlet3 -The concentration proportion of-N and organic carbon is 1:6.
Further, the simulated wastewater composition：400~700mgL of nitrate nitrogen-1, 2400~4200mgL of organic carbon-1,
KH2PO4 10mg·L-1, CaCl2·2H2O 5.6mg·L-1, MgSO4·7H2O 300mg·L-1, KHCO3 1250mg·L-1With
Micro- I, II, solvent are distilled water；
The trace element I is formed：EDTA 5.00g·L-1, FeSO49.14g·L-1；
The trace element II is formed：EDTA 15.0g·L-1, ZnSO4·7H2O 0.430g·L-1, CoCl2·6H2O
0.240g·L-1, MnCl2·4H2O 0.990g·L-1, CuSO4·5H2O 0.250g·L-1, NaMoO4·2H2O 0.220g·
L-1, NiCl2·6H2O 0.210g·L-1, H3BO40.014g·L-1。
Advantages of the present invention is mainly reflected in：The present invention is resistant to sulfamethoxazole by the method culture that gradient is tamed
10mg·L-1Denitrification granular sludge, it is ensured that denitrification process stable operation.
(4) it illustrates
Fig. 1 is the structure diagram of upflow anaerobic sludge blanket reactor；In Fig. 1：1. lower cone；2. water inlet；It is 3. anti-
Answer device main body；Frustum on 4.；5. settling zone；6. water outlet；7. three phase separator.
Reactor sludge activity changes in Fig. 2 incubations.
(5) specific embodiment
With reference to specific embodiment, the present invention is further described, but protection scope of the present invention is not construed as
The concrete form that embodiment is stated is only limitted to, protection scope of the present invention is also and in those skilled in the art according to structure of the present invention
Think it is conceivable that equivalent technologies mean.
Embodiment 1：
The up flow anaerobic sludge blanket reactor (Fig. 1) that effective volume is 0.5L is taken, inoculation experiments room activity is 660mg
N·g-1VSS·d-1, grain size be 2.77 ± 0.94mm denitrification granular sludge, be inoculated with post-reactor in suspended sediment concentration and
Volatile suspended solids concentration is 58gL respectively-1And 38gL-1.Reactor anaerobism, be protected from light, temperature for 35 ± 1 DEG C, into
Water pH is operation (Fig. 2) under conditions of 7.31 ± 0.07, hydraulic detention time is 2.4h.
Simulated wastewater ingredient is as follows in operational process：
The simulated wastewater composition：Nitrate nitrogen 700mgL-1, organic concentration of carbon (provides) 4200mgL by glucose-1,
KH2PO4 10mg·L-1, CaCl2·2H2O 5.6mg·L-1, MgSO4·7H2O 300mg·L-1, KHCO3 1250mg·L-1With
Micro- I, II, solvent are distilled water；
The trace element I is formed：EDTA 5.00g·L-1, FeSO4 9.14g·L-1；
The trace element II is formed：EDTA 15.0g·L-1, ZnSO4·7H2O 0.430g·L-1, CoCl2·6H2O
0.240g·L-1, MnCl2·4H2O 0.990g·L-1, CuSO4·5H2O 0.250g·L-1, NaMoO4·2H2O 0.220g·
L-1, NiCl2·6H2O 0.210g·L-1, H3BO4 0.014g·L-1。
Reactor stable operation started gradient domestication strategy to the 24th day.0.1mgL is added into water inlet first-1Sulphur
Amine first oxazole is run 23 days, and sludge activity is 605.8 ± 8.3mg Ng in reactor-1VSS·d-1；Then increase in water inlet
The concentration of sulfamethoxazole is to 0.2mgL-1, run 13 days, sludge activity is 514.1 ± 46.1mg Ng in reactor- 1VSS·d-1；Then increase the concentration of sulfamethoxazole in water inlet to 0.4mgL-1, run 14 days, sludge activity in reactor
For 412.5 ± 62.4mg Ng-1VSS·d-1；Increase in water inlet sulfamethoxazole concentration to 1mgL-1, sludge activity reduction
To 342.3 ± 20.5mg Ng-1VSS·d-1；And then increase the concentration of sulfamethoxazole in water inlet to 2mgL-1, operation
13 days, sludge activity was 474.3 ± 33.6mg Ng in reactor-1VSS·d-1；Increase sulfamethoxazole in water inlet again
Concentration is to 4mgL-1, run 14 days, sludge activity is 477.1 ± 43.0mg Ng in reactor-1VSS·d-1；Finally increase
The concentration of sulfamethoxazole is to 10mgL in water inlet-1, run 14 days, sludge activity is 533.3 ± 23.3mg N in reactor
g-1VSS·d-1.At this point, sludge activity is the 80% of initial sludge activity and remains unchanged for 15 days in reactor, tolerance sulphur is obtained
The denitrification granular sludge of amine first oxazole.The constant of nitrate and concentration of glucose in water inlet is kept, as sulfamethoxazole is dense
The increase of degree, sludge activity presentation first increase the trend reduced afterwards, this shows that denitrification sludge itself has one to sulfamethoxazole
Fixed adaptability.Therefore, denitrification granular sludge is resistant to 10mgL gradually being tamed through appropriate sulfamethoxazole-1Sulphur
Amine first oxazole.
Claims (8)
1. a kind of cultural method for the denitrification granular sludge for being resistant to sulfamethoxazole, it is characterised in that the method is：Using
Up flow anaerobic sludge blanket reactor, using denitrification granular sludge as seed sludge, to contain nitrate nitrogen, organic carbon, maintain micro- life
The simulated wastewater of inorganic salt buffer and trace element needed for object growth is water inlet, is 35 ± 1 DEG C, intakes in anaerobism, temperature
PH is 7.2~7.4, hydraulic detention time is stable under conditions of being 2~3h, and 0.1mgL is added into water inlet-1Sulfalene
Oxazole when running into reactor 70~80% of sludge activity for seed sludge activity, then passes through mode incremental stage by stage
Sulfamethoxazole is added into reactor water inlet, is run 13~15 days under each concentration, when denitrification sludge activity in reactor
Reach more than 70% seed sludge activity, and when sludge activity no longer reduces for 13-15 days in reactor, obtain and be resistant to sulfanilamide (SN)
The denitrification granular sludge of first oxazole.
2. the cultural method of the denitrification granular sludge of tolerance sulfamethoxazole as described in claim 1, it is characterised in that described
A concentration of 0.1~10mgL of sulfamethoxazole in water inlet-1。
3. the cultural method of the denitrification granular sludge of tolerance sulfamethoxazole as claimed in claim 2, it is characterised in that described
Sulfamethoxazole adds 2~5 times of a concentration of concentration on last stage, is run 13~15 days under each concentration.
4. the cultural method of the denitrification granular sludge of tolerance sulfamethoxazole as claimed in claim 3, it is characterised in that sulfanilamide (SN)
First oxazole adding method is：0.1mgL is added into water inlet for the first time-1Sulfamethoxazole, run dirty to denitrification in reactor
Mud activity reaches the 70~80% of seed sludge activity, then adds 0.2mgL into water inlet respectively-1、0.4mg·L-1、
1mg·L-1、2mg·L-1、10mg·L-1Sulfamethoxazole, run 13~15 days under each concentration, sludge activity in reactor
It remains unchanged for the 80% of initial sludge activity and 13~15 days, obtains the denitrification granular sludge of tolerance sulfamethoxazole.
5. the cultural method of the denitrification granular sludge of tolerance sulfamethoxazole as described in claim 1, it is characterised in that described
The activity of seed sludge is 420~680mg Ng-1VSS·d-1, grain size is 1.8~3.6mm, is inoculated in post-reactor and suspends
Solid concentration and volatile suspended solids concentration are 40~60gL respectively-1With 30~40gL-1。
6. the cultural method of the denitrification granular sludge of tolerance sulfamethoxazole as described in claim 1, it is characterised in that described
Origin of organic carbon is in the one or more of glucose, sodium acetate, methanol or molasses in simulated wastewater.
7. the cultural method of the denitrification granular sludge of tolerance sulfamethoxazole as described in claim 1, it is characterised in that described
NO in water inlet3 -The concentration proportion of-N and organic carbon is 1:6.
8. the cultural method of the denitrification granular sludge of tolerance sulfamethoxazole as described in claim 1, it is characterised in that described
Simulated wastewater forms：400~700mgL of nitrate nitrogen-1, 2400~4200mgL of organic carbon-1, KH2PO4 10mg·L-1,
CaCl2·2H2O 5.6mg·L-1, MgSO4·7H2O 300mg·L-1, KHCO3 1250mg·L-1It is molten with micro- I, II
Agent is distilled water；
The trace element I is formed：EDTA 5.00g·L-1, FeSO49.14g·L-1；
The trace element II is formed：EDTA 15.0g·L-1, ZnSO4·7H2O 0.430g·L-1, CoCl2·6H2O
0.240g·L-1, MnCl2·4H2O 0.990g·L-1, CuSO4·5H2O 0.250g·L-1, NaMoO4·2H2O 0.220g·
L-1, NiCl2·6H2O 0.210g·L-1, H3BO40.014g·L-1。
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110104768A (en) * | 2019-04-18 | 2019-08-09 | 杭州师范大学 | A kind of cultural method for the denitrification sludge being resistant to nano material |
| CN116022915A (en) * | 2023-01-28 | 2023-04-28 | 北京工业大学 | Method for applying antibiotic stress memory driven aerobic granular sludge to medical wastewater denitrification |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105692896A (en) * | 2016-04-19 | 2016-06-22 | 杭州师范大学 | Cultivation method of denitrification granular sludge resistant to copper ions |
-
2017
- 2017-12-30 CN CN201711490120.4A patent/CN108264144A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105692896A (en) * | 2016-04-19 | 2016-06-22 | 杭州师范大学 | Cultivation method of denitrification granular sludge resistant to copper ions |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110104768A (en) * | 2019-04-18 | 2019-08-09 | 杭州师范大学 | A kind of cultural method for the denitrification sludge being resistant to nano material |
| CN110104768B (en) * | 2019-04-18 | 2021-12-21 | 杭州师范大学 | Culture method of nano-material-tolerant denitrifying sludge |
| CN116022915A (en) * | 2023-01-28 | 2023-04-28 | 北京工业大学 | Method for applying antibiotic stress memory driven aerobic granular sludge to medical wastewater denitrification |
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