CN110090556A - A kind of protein electrophorese glue gel making device - Google Patents
A kind of protein electrophorese glue gel making device Download PDFInfo
- Publication number
- CN110090556A CN110090556A CN201910425813.8A CN201910425813A CN110090556A CN 110090556 A CN110090556 A CN 110090556A CN 201910425813 A CN201910425813 A CN 201910425813A CN 110090556 A CN110090556 A CN 110090556A
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- China
- Prior art keywords
- glue
- sliding panel
- groove body
- compoboard
- branch
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D57/00—Separation, other than separation of solids, not fully covered by a single other group or subclass, e.g. B03C
- B01D57/02—Separation, other than separation of solids, not fully covered by a single other group or subclass, e.g. B03C by electrophoresis
Abstract
The invention discloses a kind of protein electrophorese glue gel making device, including groove body, match glue compoboard and sliding panel, the groove body bottom is equipped with gummosis ditch；Sliding panel is equipped with inside groove body, the groove body is divided into glue branch and compresses branch's two parts, hold-down mechanism is provided in the groove body space for compressing branch, the sliding panel is equipped with injecting glue slot in the side with glue branch, the sliding panel lower end is provided with small lugs, the small lugs are inserted into the gummosis ditch and fit closely with the gummosis ditch, and the injecting glue slot is communicated with the gummosis ditch；The hold-down mechanism drives the sliding panel to slide back and forth along the gummosis ditch.The present invention can disposably prepare any number of electrophoresis offset plates, greatly improve glue efficiency.
Description
Technical field
The present invention relates to a kind of protein electrophorese glue gel making devices, belong to field of biotechnology.
Background technique
Protein electrophorese, most common is polyacrylamide gel electrophoresis (PAGE).Dodecyl sodium sulfate (SDS)
A kind of anionic surfactant, in conjunction with protein after make a large amount of negative electrical charges on protein denaturation band.It is generallyd use not
Running gel is made with upper layer glue (concentration glue) and the gel of lower layer's glue (separation gel) two kinds of various concentrations in continuous electrophoresis system
Plate achievees the effect that protein high-resolution separation by its charge effect, concentration effect and molecular sieving effect.It is denaturalized through SDS
For negatively charged protein under electric field action, the mobility speed on electrophoresis offset plate is only related with its molecular weight afterwards, therefore, can
With the protein component of different molecular weight is separated by electrophoresis at narrow zone, its electrophoresis is recorded with detection method appropriate
Zone map calculates its content (%).
The core of protein electrophorese method is the preparation of running gel, and traditional running gel gum-making rack Inner Constitution is relatively simple,
One gum-making rack can only match 1 piece of glue, and easily be worn using the materials such as organic glass, ABS, aging, damage, even if matching glue every time
Preceding leak detection, leak adhesive phenomenon happens occasionally when with glue, causes to match glue every time time-consuming and laborious.On the other hand, the device match glue when, under
The separation gel of layer must also be injected from top, easily cause separation gel injection uneven in this way, upper layer glue can inevitably be mixed with lower layer's glue
It closes, causes to fail with glue.Moreover, traditional gum-making rack can only match one piece every time, the size of each block of running gel is difficult to control accurately, no
It is larger with the batch error between running gel.
Summary of the invention
1. technical problems to be solved
The purpose of the present invention is to solve running gel gum-making rack Inner Constitution in the prior art is simple, a gum-making rack is only
The problem of energy matches 1 piece of glue, and glue easily fails, error is big between manufactured electrophoresis offset plate batch, and quality is difficult to control accurately, proposition
A kind of protein electrophorese glue gel making device.
2. technical solution
A kind of protein electrophorese glue gel making device, including groove body, match glue compoboard and sliding panel, the groove body bottom is equipped with
Gummosis ditch；It is equipped with sliding panel inside groove body, the groove body is divided into glue branch and compresses branch's two parts, in the pressure
Hold-down mechanism is provided in the groove body space of tight branch, the sliding panel is equipped with injecting glue slot in the side with glue branch,
The sliding panel lower end is provided with small lugs, and the small lugs are inserted into the gummosis ditch and closely paste with the gummosis ditch
It closes, the injecting glue slot is communicated with the gummosis ditch；The hold-down mechanism drives the sliding panel along sliding before and after the gummosis ditch
It is dynamic；
Described to be made of with glue compoboard bottom plate, side strip and cover board, two side strips are fixed on the bottom plate
Two sides cover the cover board on the upside of the side strip, are formed between side strip and cover board in the bottom plate, two empty with glue
Between；
By it is multiple be superimposed together with glue compoboard after, be put into described in glue branch, the bottom with glue compoboard
Side and the bottom of the groove body fit closely, described consistent with the sliding panel with glue compoboard placement direction, the compacting machine
It is described with glue compoboard that structure pushes the sliding panel to compress.
Preferably, the hold-down mechanism is made of screw rod, handle, and one end of the screw rod and the sliding panel are in compression point
The side in portion is connected, and the screw rod stretches out outside groove body after being engaged by the screw hole offered on the groove body side wall, the screw rod
One end outside the groove body is fixed with handle, and rotating the handle can push the sliding panel to slide back and forth.
Preferably, the hold-down mechanism, which can be to be arranged in, compresses the spring in branch or the elastomer similar to spring.
Preferably, the groove body two sides are also provided with guide groove, and the sliding board ends are equipped with locating piece, the locating piece
And energy chimeric with the guide groove is free to slide.
Preferably, the injecting glue slot top on the sliding panel offers gum-injecting port.
Preferably, the number with glue compoboard (2) is greater than 1, more preferable 3~8.
The invention also provides a kind of application methods of protein electrophorese glue gel making device, comprising the following steps:
Step 1, the number for preparing protein electrophorese glue simultaneously as needed, by being inserted into glue compoboard for corresponding number
Groove body is matched inside glue branch；
Step 2, hold-down mechanism is driven, will be fixed on glue compoboard with inside glue branch, and fit closely between each other；
Step 3, match glue from the injecting glue slot top gum-injecting port injection separation gel on sliding panel, the separation gel is suitable with glue
Glue groove of showwing loving care for flows into gummosis ditch, then flows into each matching in glue space with glue compoboard；
Step 4, it stands, separation gels is waited to solidify with glue, enter next step if necessary, otherwise skip step 5；
Step 5, concentration glue is injected separately into glue with glue space top from each with glue compoboard, stand, wait concentration
Glue is solidified with glue；
Step 6, hold-down mechanism is unclamped, takes out and matches glue compoboard, remove cover board, pour out protein electrophorese glue.
3. beneficial effect
Compared with the prior art, the present invention has the advantages that
(1) in the present invention, any number of electrophoresis offset plates can be disposably prepared, glue efficiency is greatly improved.
(2) in the present invention, the separation glue of electrophoresis offset plate is injected from lower layer, and concentration glue is injected from top, is effectively kept away
Exempt from the mixing and interference of two kinds of glue.
(3) in the present invention, multiple electrophoresis offset plates are disposably prepared, it is completely the same between each other, it can farthest keep away
Exempt from the error between batch, in advance the quality of electrophoresis offset plate.
Detailed description of the invention
Fig. 1 is a kind of one embodiment structural schematic diagram of protein electrophorese glue gel making device proposed by the present invention；
Fig. 2 is a kind of another example structure schematic diagram of protein electrophorese glue gel making device proposed by the present invention；
Fig. 3 is the structural schematic diagram of groove body, wherein (a) is the front view of groove body, it (b) is the A-A cross-sectional view of groove body；
Fig. 4 is the structural schematic diagram with glue compoboard, wherein (a) is the front view with glue compoboard, (b) for glue combination
The B-B cross-sectional view of plate；
Fig. 5 is the structural schematic diagram of sliding panel, wherein (a) is the front view of sliding panel, it (b) is the C-C section view of sliding panel
Figure.
In figure: 1 groove body, 2 match glue compoboard, 3 sliding panels, 4 hold-down mechanisms, 101 match glue branch, 102 compress branches, 103
Gummosis ditch, 104 screw holes, 105 guide grooves, 201 bottom plates, 202 side strips, 203 cover boards, 204 match glue space, 301 injecting glue slots, 302
Small lugs, 303 locating pieces, 304 gum-injecting ports, 401 screw rods, 402 handles, 403 springs.
Specific embodiment
Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, complete
Site preparation description, it is clear that described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.
In the description of the present invention, it is to be understood that, term " on ", "lower", "front", "rear", "left", "right", "top",
The orientation or positional relationship of the instructions such as "bottom", "inner", "outside" is to be based on the orientation or positional relationship shown in the drawings, merely to just
In description the present invention and simplify description, rather than the device or element of indication or suggestion meaning must have a particular orientation, with
Specific orientation construction and operation, therefore be not considered as limiting the invention.
Embodiment 1:
Referring to attached drawing 1,3-5, a kind of protein electrophorese glue gel making device, including groove body 1, match glue compoboard 2 and sliding panel
3.As shown in figure 3, the bottom of groove body 1 offers gummosis ditch 103, two sidewalls offer guide groove 105, in an end slot of groove body 1
A screw hole 104 is opened up on wall.Sliding panel 3 as shown in Figure 5, width is consistent or smaller with the width dimension of groove body 1,
A small lugs 302 are equipped in the bottom end of sliding panel 3, the size of small lugs 302 is consistent or smaller with gummosis ditch 103, sliding panel 3
Both ends be respectively provided with a locating piece 303, the size of locating piece 303 is consistent or smaller with guide groove 105, the one of sliding panel 3
An injecting glue slot 301 is opened in side, and width dimensions are consistent or smaller with gummosis ditch 103, opens up one on the top of injecting glue slot 301 and is in
The gum-injecting port 304 of bell shape facilitates injection with glue, sliding panel 3 is inserted into groove body 1, sliding panel 3 has injecting glue slot
The one end of 301 side towards groove body without screw hole, small lugs 302, which are just embedded into gummosis ditch, to be fitted closely and can free skating
Dynamic, the injecting glue slot 301 on sliding panel 3 is communicated with gummosis ditch 103, and the locating piece 303 at 3 both ends of sliding panel is also just fitted to groove body
In guide groove 105 on 1 two sidewalls.Groove body 1 is divided into two parts by the insertion of sliding panel 3,301 institute of injecting glue slot on sliding panel 3
Towards side groove body 1 be referred to as match glue branch 101, back to side be referred to as compress branch 102.Sliding panel 3 is in compression point
The side in portion 102 connects a screw rod 401, which stretches out outside groove body 1 after being engaged by the screw hole 104 on groove body 1,
One handle 402 is installed, rotational handle 402 can drive sliding panel 3 to slide, due to gummosis ditch 103 and guiding on external one end
The limitation of slot 105, sliding panel 3 can only slide back and forth.
As shown in figure 4, being made of with glue compoboard 2 bottom plate 201, two side strips 202, cover boards 203, two side strips
202 are fixed on the both sides of bottom plate 201, and the thickness of the distance between two side strips 202 and its side strip 202 is by required preparation
The thickness and size of running gel determine.One, which is just combined into, in one cover board 203 of upper side cover of side slat item 202 matches glue compoboard 2,
The space that bottom plate 201, side strip 202 and cover board 203 are enclosed be match glue space 204, the size and shape with glue space 204 with
The size and shape of the running gel prepared needed for us is completely the same.
As shown in Figure 1, by 3 combine stacked with glue compoboard 2 and be put into above-mentioned in glue branch 101, put
The direction set is consistent with the direction of sliding panel 3, and the bottom with glue compoboard 2 and the bottom of groove body 1 fit closely, rotational handle
402,3 forward slip of sliding panel is driven, makes 3 to compress between each other with glue compoboard 2, gum-injecting port 304, injecting glue slot 301, gummosis
Ditch, each glue space 204 of matching on glue compoboard 2 form a space being connected.
A kind of application method of protein electrophorese glue gel making device in the present embodiment, comprising the following steps:
Step 1, by 3 assemble glue compoboard 2 be inserted into groove body 1 with inside glue branch 101；
Step 2, rotational handle 402 drive 3 forward slip of sliding panel, will be fixed on glue compoboard 2 with glue branch 101
It is interior, and fit closely between each other；
Step 3, match glue from 301 top gum-injecting port 304 of the injecting glue slot injection separation gel on sliding panel 3, due to sliding panel 3
The blocking of lower part small lugs 302, separation gel may not flow into the compression branch 102 of groove body 1 with glue, can only as shown in Figure 1
Arrow direction flows into gummosis ditch 103 from gum-injecting port 304 along injecting glue slot 301, then flow into it is each with glue compoboard 2 with glue
It is each completely the same with the separation glue height in glue space 204 according to law of connected vessels in space 204；
Step 4, it stands, separation gels is waited to solidify with glue；
Step 5, concentration glue is injected separately into glue with 204 top of glue space from each with glue compoboard 2, stand, etc.
Glue is concentrated to solidify with glue；
Step 6, opposite direction rotational handle 402 unclamps sliding panel 3, takes out and matches glue compoboard 2, removes cover board 203, pour out
Protein electrophorese glue, 3 pieces of running gel sizes disposable while obtained are identical.
Embodiment 2:
Referring to attached drawing 2-5, a kind of protein electrophorese glue gel making device, including groove body 1, match glue compoboard 2 and sliding panel 3.
As shown in figure 3, the bottom of groove body 1 offers gummosis ditch 103.Sliding panel 3 as shown in Figure 5, the inside of width and groove body 1 are wide
It is consistent or smaller to spend size, is equipped with a small lugs 302, the size and gummosis ditch 103 of small lugs 302 in the bottom end of sliding panel 3
It is consistent or smaller, an injecting glue slot 301 is opened in the side of sliding panel 3, width dimensions are consistent or smaller with gummosis ditch 103,
The top of injecting glue slot 301 opens up a gum-injecting port 304 in bell shape, facilitates injection with glue, sliding panel 3 is inserted into
In groove body 1, small lugs 302, which are just embedded into gummosis ditch, to be fitted closely and can free to slide, the injecting glue slot 301 on sliding panel 3
It is communicated with gummosis ditch 103.Groove body 1 is divided into two parts by the insertion of sliding panel 3, on sliding panel 3 injecting glue slot 301 towards
Side groove body 1 be referred to as match glue branch 101, back to side be referred to as compress branch 102.It is being slided compressing in branch 102
One spring 403 is set between plate 3 and the end wall of groove body 1.Stretching, extension and compressed spring 403, can drive sliding panel 3 to slide back and forth.
As shown in figure 4, being made of with glue compoboard 2 bottom plate 201, two side strips 202, cover boards 203, two side strips
202 are fixed on the both sides of bottom plate 201, and the thickness of the distance between two side strips 202 and its side strip 202 is by required preparation
The thickness and size of running gel determine.One, which is just combined into, in one cover board 203 of upper side cover of side slat item 202 matches glue compoboard 2,
The space that bottom plate 201, side strip 202 and cover board 203 are enclosed be match glue space 204, the size and shape with glue space 204 with
The size and shape of the running gel prepared needed for us is completely the same.
As shown in Fig. 2, by 8 combine stacked with glue compoboard 2 and be put into above-mentioned in glue branch 101, put
The direction set is consistent with the direction of sliding panel 3, and the bottom with glue compoboard 2 and the bottom of groove body 1 fit closely, in spring 403
Under the action of compress sliding panel 3, make 8 with glue compoboard 2 between each other compress, gum-injecting port 304, injecting glue slot 301, gummosis ditch,
Each glue space 204 of matching on glue compoboard 2 forms a space being connected.
A kind of application method of protein electrophorese glue gel making device in the present embodiment, comprising the following steps:
Step 1, by 8 assemble glue compoboard 2 be inserted into groove body 1 with inside glue branch 101；
Step 2, sliding panel 3 is compressed under the action of spring 403, will be fixed on glue compoboard 2 in glue branch 101,
And it fits closely between each other；
Step 3, match glue from 301 top gum-injecting port 304 of the injecting glue slot injection separation gel on sliding panel 3, due to sliding panel 3
The blocking of lower part small lugs 302, separation gel may not flow into the compression branch 102 of groove body 1 with glue, can only as shown in Figure 2
Arrow direction flows into gummosis ditch 103 from gum-injecting port 304 along injecting glue slot 301, then flow into it is each with glue compoboard 2 with glue
It is each completely the same with the separation glue height in glue space 204 according to law of connected vessels in space 204.
Step 4, it stands, separation gels is waited to solidify with glue；
Step 5, sliding panel 3 is unclamped in compressed spring 403, takes out and matches glue compoboard 2, removes cover board 203, pour out protein
Running gel, 8 pieces of running gel sizes disposable while obtained are identical.
The foregoing is only a preferred embodiment of the present invention, but scope of protection of the present invention is not limited thereto,
Anyone skilled in the art in the technical scope disclosed by the present invention, according to the technique and scheme of the present invention and its
Inventive concept is subject to equivalent substitution or change, should be covered by the protection scope of the present invention.
Claims (7)
1. a kind of protein electrophorese glue gel making device, including groove body (1), match glue compoboard (2) and sliding panel (3), feature exists
Gummosis ditch (103) are equipped in the groove body (1) bottom；Sliding panel (3) are equipped with inside groove body (1), the groove body (1) is divided
At with glue branch (101) and compression branch (102) two parts, pressure is provided in the groove body space for compressing branch (102)
Tight mechanism (4), the sliding panel (3) is equipped with injecting glue slot (301) in the side with glue branch (101), in the sliding panel
(3) lower end is provided with small lugs (302), the small lugs (302) be inserted into the gummosis ditch (103) and with the gummosis ditch
(103) it fits closely, the injecting glue slot (301) communicates with the gummosis ditch (103)；The hold-down mechanism (4) drives the cunning
Movable plate (3) slides back and forth along the gummosis ditch (103)；
It is described to be made of with glue compoboard (2) bottom plate (201), side strip (202) and cover board (203), two side strips
(202) two sides of the bottom plate (201) are fixed on, the cover board (203) are covered on the upside of the side strip (202), in institute
It states and is formed between bottom plate (201), two side strips (202) and cover board (203) with glue space (204)；
By it is multiple be superimposed together with glue compoboard (2) after, be put into it is described in glue branch (101), it is described to match glue compoboard
(2) bottom of bottom side and the groove body (1) fits closely, described to match glue compoboard (2) placement direction and the sliding panel (3)
Unanimously, the hold-down mechanism (4) pushes the sliding panel (3) to compress described with glue compoboard (2).
2. gel making device according to claim 1, it is characterised in that the hold-down mechanism (4) is by screw rod (401), handle
(402) it forms, one end of the screw rod (401) is connected with the sliding panel (3) in the side for compressing branch (102), the spiral shell
Bar (4) stretches out the groove body (1) outside after engaging by the screw hole (104) offered on the groove body (1) side wall, the screw rod
(4) handle (402) are fixed in the external one end of groove body (1), the sliding panel (3) can be pushed by rotating the handle (402)
It slides back and forth.
3. gel making device according to claim 1, it is characterised in that the hold-down mechanism (4) can be setting in compression point
Spring in portion (102) or the elastomer (403) similar to spring.
4. gel making device according to claim 1, it is characterised in that groove body (1) two sides are also provided with guide groove
(105), sliding panel (3) both ends be equipped with locating piece (303), the locating piece (303) it is chimeric with the guide groove (105) and
It can be free to slide.
5. gel making device according to claim 1, it is characterised in that on the injecting glue slot (301) on the sliding panel (3)
Portion offers gum-injecting port (304).
6. gel making device according to claim 1, it is characterised in that the number with glue compoboard (2) is excellent greater than 1
Select 3~8.
7. a kind of application method of protein electrophorese glue gel making device, it is characterised in that the following steps are included:
Step 1, the number for preparing protein electrophorese glue simultaneously as needed, by being inserted into glue compoboard (2) for corresponding number
Groove body (1) matches glue branch (101) inside；
Step 2, hold-down mechanism (4) are driven, will be fixed on glue compoboard (2) with glue branch (101) inside, it is close between each other
Fitting；
Step 3, match glue, the separation from injecting glue slot (301) top gum-injecting port (304) the injection separation gel on sliding panel (3)
Glue flows into gummosis ditch (103) along injecting glue slot (301) with glue, then flow into it is each with glue compoboard (2) with glue space
(204) in；
Step 4, it stands, separation gels is waited to solidify with glue, enter next step if necessary, otherwise skip step 5；
Step 5, concentration glue is injected separately into glue with glue space (204) top from each with glue compoboard (2), stand, etc.
Glue is concentrated to solidify with glue；
Step 6, hold-down mechanism (4) are unclamped, takes out and match glue compoboard (2), removes cover board (203), pour out protein electrophorese glue.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910425813.8A CN110090556B (en) | 2019-05-21 | 2019-05-21 | Protein electrophoresis gel preparation device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910425813.8A CN110090556B (en) | 2019-05-21 | 2019-05-21 | Protein electrophoresis gel preparation device |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN110090556A true CN110090556A (en) | 2019-08-06 |
| CN110090556B CN110090556B (en) | 2023-10-17 |
Family
ID=67448721
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910425813.8A Active CN110090556B (en) | 2019-05-21 | 2019-05-21 | Protein electrophoresis gel preparation device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110090556B (en) |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN204196082U (en) * | 2014-10-31 | 2015-03-11 | 宁波市鄞州祥弘电子有限公司 | A kind of protein gel separation gel plate forming device |
| CN108709924A (en) * | 2018-06-28 | 2018-10-26 | 徐涛 | A kind of quick detachable gel making device in situ |
-
2019
- 2019-05-21 CN CN201910425813.8A patent/CN110090556B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN204196082U (en) * | 2014-10-31 | 2015-03-11 | 宁波市鄞州祥弘电子有限公司 | A kind of protein gel separation gel plate forming device |
| CN108709924A (en) * | 2018-06-28 | 2018-10-26 | 徐涛 | A kind of quick detachable gel making device in situ |
Also Published As
| Publication number | Publication date |
|---|---|
| CN110090556B (en) | 2023-10-17 |
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