CN110090323A - A kind of beta-tricalcium phosphate porous ceramic carried stent and its preparation method and application - Google Patents
A kind of beta-tricalcium phosphate porous ceramic carried stent and its preparation method and application Download PDFInfo
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- CN110090323A CN110090323A CN201910381158.0A CN201910381158A CN110090323A CN 110090323 A CN110090323 A CN 110090323A CN 201910381158 A CN201910381158 A CN 201910381158A CN 110090323 A CN110090323 A CN 110090323A
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- tricalcium phosphate
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- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
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- A61L27/12—Phosphorus-containing materials, e.g. apatite
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Abstract
The present invention relates to a kind of beta-tricalcium phosphate porous ceramic carried stent and preparation method and application, comprising the following steps: (1) prepares with macroscopical macropore and the internal beta-tricalcium phosphate porous ceramic bracket with microcosmic aperture；(2) silk fibroin protein solution is prepared, drug to be loaded is added, is uniformly mixed, obtains the mixed solution of fibroin albumen and drug；(3) ultrasound is carried out to above-mentioned mixed solution, the load medicine silk fibroin protein solution is fed into beta-tricalcium phosphate porous ceramic bracket rapidly after a period of time, water-bath under room temperature after perfusion waits silk fibroin protein solution at gel solidification；(4) take out above-mentioned sample and carry out freeze-drying process, the extra fibroin albumen of removal bata-tricalcium phosphate porous scaffold surface to get.Compared with prior art, the beneficial effects of the present invention are: (1) good biocompatibility；(2) better Bone Defect Repari effect；(3) there is lasting drug release effect.
Description
Technical field
The present invention relates to a kind of beta-tricalcium phosphate porous ceramic carried stent and preparation method and application, belong to biomaterial neck
Domain.
Background technique
Bone tissue engineer is considered as the effective means for solving bone tissue regeneration and repairing problem.Bata-tricalcium phosphate mainly at
Divide and is made of calcium and phosphorus, it is similar to body bone tissue inorganic constituents, there is good biocompatibility, osteoacusis and self-bone grafting energy
Power；Bata-tricalcium phosphate can be degraded by tissue, and the Calcium and phosphorous component being degraded participates in freshman bone tissue by bone steady-state adjustment
In regeneration and reconstruction.However, bata-tricalcium phosphate material structure repairing effect is slow, have a single function, therefore by β-tricresyl phosphate
Loading functional drug in calcium bracket can test the acceleration reparation of bone tissue and realize other functional purposes.
Fibroin albumen is the natural polymer fibrin extracted from silk, contains amino acid in 18, is had good
Biocompatibility and rush vascular[3], the degradation time of the Silk fibroin gel of various concentration in vivo is several thoughtful some months,
Thus, using Silk fibroin gel as drug loading material[3], it is compound with porous beta-calcium phosphate ceramics bracket, from structure
The mechanical property of bracket can be enhanced；Functionally, it with the gradually degradation of bracket, carries medicine fibroin albumen and is further exposed to group
Knit in liquid, can be realized sustained drug release, and then promote blood vessel grow into and at Bone Defect Repari effect, have it is very high research and
Application value.
Summary of the invention
The purpose of the present invention is to provide a kind of beta-tricalcium phosphate porous ceramic carried stent and preparation methods, can make
For the application in bone renovating material.
Technical scheme is as follows: a kind of preparation method of beta-tricalcium phosphate porous ceramic carried stent, feature
It is, comprising the following steps:
(1) it prepares with macroscopical macropore and the internal beta-tricalcium phosphate porous ceramic bracket with microcosmic aperture；
(2) silk fibroin protein solution is prepared, drug to be loaded is added, is uniformly mixed, obtains fibroin albumen and drug
Mixed solution；
(3) ultrasound is carried out to above-mentioned mixed solution, the load medicine silk fibroin protein solution is fed into β-rapidly after a period of time
Tricalcium phosphate porous ceramic bracket, water-bath under room temperature after perfusion wait silk fibroin protein solution at gel solidification；
(4) it takes out above-mentioned sample and carries out freeze-drying process, the extra fibroin of removal bata-tricalcium phosphate porous scaffold surface
Albumen to get.According to the above scheme, in step (1) macroscopical macropore of beta-tricalcium phosphate porous ceramic bracket aperture be 300 μm~
800 μm, the aperture of microcosmic aperture is 1 μm~5 μm, and macroscopical macropore is connected to microcosmic aperture height.
According to the above scheme, beta-tricalcium phosphate porous ceramic bracket is prepared by three-dimensional printing technology in step (1),
The following steps are included:
(1) bata-tricalcium phosphate powder, sodium alginate soln, pluronic F-127 solution and ultrapure water are stirred by ball milling
It mixes uniformly, obtains 3D printing ink；
(2) 3D printing ink is put into printing device, designs three-dimensional rack model and print parameters, passes through 3 D-printing skill
Art prints porous ceramics；
(3) porous ceramics scaffold printed is sintered after air drying to get.
According to the above scheme, by mass parts, the proportion of each raw material are as follows: 30~44 parts of bata-tricalcium phosphate powder, 18~25
The sodium alginate soln that part mass concentration is 5%~20%, the pluronic F-127 that 3~9 parts of mass concentrations are 10%~30%
Solution, 0~7 part of ultrapure water.
According to the above scheme, the sintering schedule in step (3) are as follows: rise to 500 DEG C~550 DEG C from room temperature, keep the temperature 0.5~2h；
1000 DEG C~1100 DEG C are risen to from 500 DEG C~550 DEG C with 10~20 DEG C/min, keep the temperature 0.5~2h；With 10~20 DEG C/min from
1000 DEG C~1100 DEG C are cooled to 800 DEG C~900 DEG C, keep the temperature 2~4h；Furnace cooling to get.
According to the above scheme, the silk fibroin protein solution mass concentration that step (2) is prepared is 1%~10%.
According to the above scheme, the drug in step (2) is icariin, and the additive amount of icariin is fibroin albumen quality
1%~10%.
According to the above scheme, the power of ultrasonication is 60~150w in step (3), and ultrasonic time is 30~80s.
The above-mentioned resulting beta-tricalcium phosphate porous ceramic carried stent of any preparation method.
Purposes of the beta-tricalcium phosphate porous ceramic carried stent in bone renovating material.
Compared with prior art, the beneficial effects of the present invention are:
(1) good biocompatibility: fibroin albumen and bata-tricalcium phosphate of the present invention are biocompatibility
Good biomaterial can reduce and avoid inflammation and immune response as the carried stent of raw material preparation；
(2) better Bone Defect Repari effect: bata-tricalcium phosphate has osteoacusis and self-bone grafting ability, and can degrade in vivo
Human body metabolic cycles are participated in, sufficient calcium and phosphorus are provided for damaged bony tissues reparation；Fibroin albumen, which has, promotes new vessels raw
At ability, the formation of new vessels will provide nutriment abundant for skeletonization relevant cell, and promote the growth of bone；Fibroin egg
White medicament-carried release further promotes the formation of bone；
(3) with lasting drug release effect: with connecing for beta-tricalcium phosphate porous ceramic carried stent and tissue fluid
It touches, the fibroin albumen being exposed in tissue fluid in bracket macroscopic view macropore is gradually degraded under the action of proteolytic enzyme, and release is held
The drug of load；Further, the fibroin albumen being wrapped in the microcosmic aperture of internal stent is gradually exposed with the degradation of bracket
And be hydrolyzed, the drug of carrying is also released in turn, is reached drug and is discharged in early days comparatively fast, the later period discharges slowly the simultaneously medicine of sustained release
Object releasing effect.
Detailed description of the invention
Fig. 1 is the scanning electron microscopic appearance figure of beta-tricalcium phosphate porous ceramic bracket prepared by embodiment 1；
Fig. 2 is beta-tricalcium phosphate porous ceramic bracket scanning electron microscopic appearance figure prepared by embodiment 1；
Fig. 3 is the load medicine beta-tricalcium phosphate porous ceramic bracket and pure beta-tricalcium phosphate porous ceramic for preparing embodiment 1
Stenter to implant rat femur condyle defect point, the BV/TV statistics of the New Bone Quantity obtained after MicroCT is tested after 4 weeks and 8 weeks
Value.
Specific embodiment
In order to make the objectives, technical solutions, and advantages of the present invention clearer, below with reference to embodiment, to the present invention
It is further described.It should be appreciated that the specific embodiments are only for explaining the present invention herein, it is not intended to limit the present invention.
Bata-tricalcium phosphate of the present invention carries the defect that medicine porous ceramics scaffold is used to repair human body hard bone tissue, hole knot of the present invention
Structure design and material component proportion, loading drug are not limited to scheme of the present invention.
Embodiment 1:
(1) by 30g bata-tricalcium phosphate, 18g mass concentration be 10% sodium alginate soln, 3g mass concentration be 30%
Pluronic F-127 solution, 7g ultrapure water are uniform by ball grinding stirring, and 3D printing ink is made；3D printing ink is put into 3D
Printing device, design diameter 8mm are highly the cylinder printer model of 10mm, using jet diameters 0.41mm, air pressure
0.5MPa, print speed 3mm/s, thickness 0.4mm, adjacent spacing are the print parameters of 0.8mm, are printed by 3D printing equipment
Molding is dried 24 hours at room temperature；
(2) printed bracket is put into Muffle furnace, sintering schedule are as follows: 5 DEG C/min rises to 500 DEG C from room temperature, heat preservation
0.5 hour；10 DEG C/min rises to 1100 DEG C from 500 DEG C, keeps the temperature 0.5 hour；For 10 DEG C/min fast cooling to 800 DEG C, heat preservation 2 is small
When, furnace cooling, obtaining macroscopical macropore is 600 μm, and there are the porous lifes of the bata-tricalcium phosphate of 1~5 μm of interconnected pore for internal stent
Object ceramics bracket；
(3) silk cocoon is put into the Na that mass concentration is 0.5% at 95 DEG C2CO3Digestion 60min in solution, is repeated 3 times；?
The complete silk cocoon of degumming is dried at 50 DEG C, obtains the silk of threadiness；Dried silk is put into the bromine of 10mol/L at 60 DEG C
Change in lithium solution and dissolve 2h, is completely dissolved silk；Dissolved silk protein solution is collected, centrifugation removal impurity is put into retention
In the bag filter that amount is 3500, dialyse 3 days in ultrapure water；Then the silk fibroin protein solution after dialysis is freeze-dried,
The fibroin albumen extracted；
(4) 2g fibroin albumen is dissolved in 20g ultrapure water, stirring dissolves it sufficiently, and preparing mass concentration is 10%
Silk fibroin protein solution；0.02g icariin is added, stirring dissolves it sufficiently, obtains fibroin albumen and icariin mixing
Solution；
(5) power that ultrasonic disperse instrument is arranged is that ultrasound emission end is placed on fibroin albumen under condition of ice bath by 60W
Near icariin mixed solution liquid level, ultrasonication 60s；
(6) beta-tricalcium phosphate porous ceramic bracket is put into the three-neck flask with sealed valve, opening vacuumizes end
Valve is closed other two valves, is vacuumized using water pump to three-neck flask inside, be evacuated to vacuum meter registration be-
0.1MPa, closing vacuumize end valve door, open other end valve, make the silk fibroin protein solution suck-back of loading drug to three-neck flask
Interior submergence beta-tricalcium phosphate porous ceramic bracket waits 30min；Three-neck flask is then transferred to 37 DEG C of water-bath heat preservation 4h；
(7) it after forming opaque hydrogel after fibroin albumen, is freezed after three-neck flask is put into refrigerator freezing 12h
It is dried, removes moisture removal；Take out the sample in three-neck flask, the extra silk of removal beta-tricalcium phosphate porous ceramic rack surface
Fibroin to get；
(8) bata-tricalcium phosphate of the beta-tricalcium phosphate porous ceramic carried stent and unloaded drug of icariin will be loaded
Porous ceramics scaffold is implanted into rat femur condyle defect model, and bone defect healing result is observed after 4 weeks and 8 weeks.As shown in Figure 1,
It can be seen from the figure that the beta-tricalcium phosphate porous ceramic bracket of preparation has uniform macroscopical macropore.
As shown in Fig. 2, it can be seen from the figure that there are 1~5 μm of small interconnected pores inside ceramics bracket.
As shown in figure 3, the BV/TV statistical value of Micro-CT result shows that the bata-tricalcium phosphate for loading icariin is porous
More other groups of ceramic carried stent New Bone Quantity is significantly more, illustrates that the beta-tricalcium phosphate porous ceramic for loading icariin carries
Medicine bracket has the ability for promoting skeletonization in vivo well.
Embodiment 2:
(1) by 44g bata-tricalcium phosphate, 25g mass concentration be 20% sodium alginate soln, 9g mass concentration be 20%
Pluronic F-127 solution, 3g ultrapure water are uniform by ball grinding stirring, and 3D printing ink is made；3D printing ink is put into 3D
Printing device, design diameter 8mm are highly the cylinder printer model of 10mm, using jet diameters 0.41mm, air pressure
0.5MPa, print speed 3mm/s, thickness 0.4mm, adjacent spacing are the print parameters of 0.4mm, are printed by 3D printing equipment
Molding is dried 24 hours at room temperature；
(2) printed bracket is put into Muffle furnace, sintering schedule are as follows: 5 DEG C/min rises to 550 DEG C from room temperature, heat preservation 2
Hour；10 DEG C/min rises to 1000 DEG C from 550 DEG C, keeps the temperature 2 hours；10 DEG C/min fast cooling keeps the temperature 2 hours to 900 DEG C, with
Furnace is cooling, and obtaining macroscopical macropore is 300 μm, and there are the bata-tricalcium phosphate porous bio-ceramics of 1~5 μm of interconnected pore for internal stent
Bracket；
(3) silk cocoon is put into digestion 60min in the Na2CO3 solution that mass concentration is 0.5% at 95 DEG C, be repeated 3 times；
The complete silk cocoon of degumming is dried at 50 DEG C, obtains the silk of threadiness；Dried silk is put into 10mol/L's at 60 DEG C
2h is dissolved in lithium-bromide solution, is completely dissolved silk；Dissolved silk protein solution is collected, centrifugation removal impurity is put into and cuts
In the bag filter that allowance is 3500, dialyse 3 days in ultrapure water；It is dry that the silk fibroin protein solution after dialysis is then subjected to freezing
Fibroin albumen that is dry, being extracted；
(4) 2g fibroin albumen is dissolved in 20g ultrapure water, stirring dissolves it sufficiently, and preparing mass concentration is 10%
Silk fibroin protein solution；0.2g icariin is added, stirring dissolves it sufficiently, obtains fibroin albumen and icariin mixing is molten
Liquid；
(5) power that ultrasonic disperse instrument is arranged is that ultrasound emission end is placed on fibroin albumen under condition of ice bath by 90W
Near icariin mixed solution liquid level, ultrasonication 45s；
(6) beta-tricalcium phosphate porous ceramic bracket is put into the three-neck flask with sealed valve, opening vacuumizes end
Valve is closed other two valves, is vacuumized using water pump to three-neck flask inside, be evacuated to vacuum meter registration be-
0.1MPa, closing vacuumize end valve door, open other end valve, make the silk fibroin protein solution suck-back of loading drug to three-neck flask
Interior submergence beta-tricalcium phosphate porous ceramic bracket waits 30min；Three-neck flask is then transferred to 37 DEG C of water-bath heat preservation 4h；
(7) it after forming opaque hydrogel after fibroin albumen, is freezed after three-neck flask is put into refrigerator freezing 12h
It is dried, removes moisture removal；Take out the sample in three-neck flask, the extra silk of removal beta-tricalcium phosphate porous ceramic rack surface
Fibroin to get.
Embodiment 3:
(1) by 30g bata-tricalcium phosphate, 20g mass concentration be 10% sodium alginate soln, 5g mass concentration be 20%
Pluronic F-127 solution is uniform by ball grinding stirring, and 3D printing ink is made；3D printing ink is put into 3D printing equipment,
Design diameter is 8mm, is highly the cylinder printer model of 10mm, using jet diameters 0.41mm, air pressure 0.5MPa, print speed
3mm/s, thickness 0.4mm, adjacent spacing are that the print parameters of 0.6mm are dried at room temperature by 3D printing equipment printing shaping
24 hours；
(2) printed bracket is put into Muffle furnace, sintering schedule are as follows: 5 DEG C/min rises to 500 DEG C from room temperature, heat preservation 1
Hour；20 DEG C/min rises to 1100 DEG C from 500 DEG C, keeps the temperature 2 hours；20 DEG C/min fast cooling keeps the temperature 2 hours to 800 DEG C, with
Furnace is cooling, and obtaining macroscopical macropore is 450 μm, and there are the bata-tricalcium phosphate porous bio-ceramics of 1~5 μm of interconnected pore for internal stent
Bracket；
(3) silk cocoon is put into digestion 60min in the Na2CO3 solution that mass concentration is 0.5% at 95 DEG C, be repeated 3 times；
The complete silk cocoon of degumming is dried at 50 DEG C, obtains the silk of threadiness；Dried silk is put into 10mol/L's at 60 DEG C
2h is dissolved in lithium-bromide solution, is completely dissolved silk；Dissolved silk protein solution is collected, centrifugation removal impurity is put into and cuts
In the bag filter that allowance is 3500, dialyse 3 days in ultrapure water；It is dry that the silk fibroin protein solution after dialysis is then subjected to freezing
Fibroin albumen that is dry, being extracted；
(4) 0.2g fibroin albumen is dissolved in 20g ultrapure water, stirring dissolves it sufficiently, prepares mass concentration and is
10% silk fibroin protein solution；0.002g icariin is added, stirring dissolves it sufficiently, obtains fibroin albumen and icariin
Mixed solution；
(5) power that ultrasonic disperse instrument is arranged is that ultrasound emission end is placed on fibroin albumen under condition of ice bath by 150W
Near icariin mixed solution liquid level, ultrasonication 80s；
(6) beta-tricalcium phosphate porous ceramic bracket is put into the three-neck flask with sealed valve, opening vacuumizes end
Valve is closed other two valves, is vacuumized using water pump to three-neck flask inside, be evacuated to vacuum meter registration be-
0.1MPa, closing vacuumize end valve door, open other end valve, make the silk fibroin protein solution suck-back of loading drug to three-neck flask
Interior submergence beta-tricalcium phosphate porous ceramic bracket waits 30min；Three-neck flask is then transferred to 37 DEG C of water-bath heat preservation 4h；
(7) it after forming opaque hydrogel after fibroin albumen, is freezed after three-neck flask is put into refrigerator freezing 12h
It is dried, removes moisture removal；Take out the sample in three-neck flask, the extra silk of removal beta-tricalcium phosphate porous ceramic rack surface
Fibroin to get.
Embodiment 4:
(1) by 30g bata-tricalcium phosphate, 22g mass concentration be 5% sodium alginate soln, 5g mass concentration be 20%
Pluronic F-127 solution, 4g ultrapure water are uniform by ball grinding stirring, and 3D printing ink is made；3D printing ink is put into 3D
Printing device, design diameter 8mm are highly the cylinder printer model of 10mm, using jet diameters 0.41mm, air pressure
0.5MPa, print speed 3mm/s, thickness 0.4mm, adjacent spacing are the print parameters of 0.8mm, are printed by 3D printing equipment
Molding is dried 24 hours at room temperature；
(2) printed bracket is put into Muffle furnace, sintering schedule are as follows: 5 DEG C/min rises to 500 DEG C from room temperature, heat preservation 1
Hour；10 DEG C/min rises to 1100 DEG C from 500 DEG C, keeps the temperature 0.5 hour；10 DEG C/min fast cooling keeps the temperature 4 hours to 800 DEG C,
Furnace cooling, obtaining macroscopical macropore is 600 μm, and there are the bata-tricalcium phosphate multiporous biological of 1~5 μm of interconnected pore potteries for internal stent
Porcelain bracket；
(3) silk cocoon is put into digestion 60min in the Na2CO3 solution that mass concentration is 0.5% at 95 DEG C, be repeated 3 times；
The complete silk cocoon of degumming is dried at 50 DEG C, obtains the silk of threadiness；Dried silk is put into 10mol/L's at 60 DEG C
2h is dissolved in lithium-bromide solution, is completely dissolved silk；Dissolved silk protein solution is collected, centrifugation removal impurity is put into and cuts
In the bag filter that allowance is 3500, dialyse 3 days in ultrapure water；It is dry that the silk fibroin protein solution after dialysis is then subjected to freezing
Fibroin albumen that is dry, being extracted；
(4) 0.4g fibroin albumen is dissolved in 20g ultrapure water, stirring dissolves it sufficiently, prepares mass concentration and is
10% silk fibroin protein solution；0.02g icariin is added, stirring dissolves it sufficiently, obtains fibroin albumen and icariin
Mixed solution；
(5) power that ultrasonic disperse instrument is arranged is that ultrasound emission end is placed on fibroin albumen under condition of ice bath by 90W
Near icariin mixed solution liquid level, ultrasonication 30s；
(6) beta-tricalcium phosphate porous ceramic bracket is put into the three-neck flask with sealed valve, opening vacuumizes end
Valve is closed other two valves, is vacuumized using water pump to three-neck flask inside, be evacuated to vacuum meter registration be-
0.1MPa, closing vacuumize end valve door, open other end valve, make the silk fibroin protein solution suck-back of loading drug to three-neck flask
Interior submergence beta-tricalcium phosphate porous ceramic bracket waits 30min；Three-neck flask is then transferred to 37 DEG C of water-bath heat preservation 4h；
(7) it after forming opaque hydrogel after fibroin albumen, is freezed after three-neck flask is put into refrigerator freezing 12h
It is dried, removes moisture removal；Take out the sample in three-neck flask, the extra silk of removal beta-tricalcium phosphate porous ceramic rack surface
Fibroin to get.
Claims (10)
1. a kind of preparation method of beta-tricalcium phosphate porous ceramic carried stent, which comprises the following steps:
(1) it prepares with macroscopical macropore and the internal beta-tricalcium phosphate porous ceramic bracket with microcosmic aperture；
(2) silk fibroin protein solution is prepared, drug to be loaded is added, is uniformly mixed, obtains the mixing of fibroin albumen and drug
Solution；
(3) ultrasound is carried out to above-mentioned mixed solution, the load medicine silk fibroin protein solution is fed into β-phosphoric acid rapidly after a period of time
Tricalcium porous ceramics scaffold, water-bath under room temperature after perfusion wait silk fibroin protein solution at gel solidification；
(4) it takes out above-mentioned sample and carries out freeze-drying process, remove the extra fibroin albumen of bata-tricalcium phosphate porous scaffold surface,
To obtain the final product.
2. the preparation method of beta-tricalcium phosphate porous ceramic carried stent according to claim 1, which is characterized in that step
(1) aperture of macroscopical macropore of beta-tricalcium phosphate porous ceramic bracket is 300 μm~800 μm in, and the aperture of microcosmic aperture is 1 μm
~5 μm, and macroscopical macropore is connected to microcosmic aperture height.
3. the preparation method of beta-tricalcium phosphate porous ceramic carried stent according to claim 2, which is characterized in that step
(1) beta-tricalcium phosphate porous ceramic bracket is prepared by three-dimensional printing technology in, comprising the following steps:
(1) bata-tricalcium phosphate powder, sodium alginate soln, pluronic F-127 solution and ultrapure water is equal by ball grinding stirring
It is even, obtain 3D printing ink；
(2) 3D printing ink is put into printing device, designs three-dimensional rack model and print parameters, is beaten by three-dimensional printing technology
Print off porous ceramics；
(3) porous ceramics scaffold printed is sintered after air drying to get.
4. the preparation method of beta-tricalcium phosphate porous ceramic carried stent according to claim 3, which is characterized in that press matter
Measure part, the proportion of each raw material are as follows: 30~44 parts of bata-tricalcium phosphate powder, 18~25 parts of mass concentrations are 5%~20%
Sodium alginate soln, the pluronic F-127 solution that 3~9 parts of mass concentrations are 10%~30%, 0~7 part of ultrapure water.
5. the preparation method of beta-tricalcium phosphate porous ceramic carried stent according to claim 3, which is characterized in that step
(3) sintering schedule in are as follows: rise to 500 DEG C~550 DEG C from room temperature, keep the temperature 0.5~2h；With 10~20 DEG C/min from 500 DEG C~
550 DEG C rise to 1000 DEG C~1100 DEG C, keep the temperature 0.5~2h；800 DEG C are cooled to from 1000 DEG C~1100 DEG C with 10~20 DEG C/min
~900 DEG C, keep the temperature 2~4h；Furnace cooling to get.
6. the preparation method of beta-tricalcium phosphate porous ceramic carried stent according to claim 1, which is characterized in that step
(2) the silk fibroin protein solution mass concentration prepared is 1%~10%.
7. the preparation method of beta-tricalcium phosphate porous ceramic carried stent according to claim 1, which is characterized in that step
(2) drug in is icariin, and the additive amount of icariin is the 1%~10% of fibroin albumen quality.
8. the preparation method of beta-tricalcium phosphate porous ceramic carried stent according to claim 1, which is characterized in that step
(3) power of ultrasonication is 60~150w in, and ultrasonic time is 30~80s.
9. the resulting beta-tricalcium phosphate porous ceramic carried stent of any preparation method of claim 1-8.
10. purposes of the beta-tricalcium phosphate porous ceramic carried stent as claimed in claim 9 in bone renovating material.
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| CN114533968A (en) * | 2022-01-12 | 2022-05-27 | 重庆医科大学 | Vascularizable stent and preparation method thereof |
| CN114712557A (en) * | 2022-04-02 | 2022-07-08 | 中山大学附属口腔医院 | Hydrogel reinforced biological bone calcium apatite scaffold and manufacturing method thereof |
| CN115054729A (en) * | 2022-07-07 | 2022-09-16 | 广州贝奥吉因生物科技股份有限公司 | Double-network hydrogel and preparation method and application thereof |
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Application publication date: 20190806 |