CN110075938A - A kind of liquid separation sample injector - Google Patents
A kind of liquid separation sample injector Download PDFInfo
- Publication number
- CN110075938A CN110075938A CN201910441970.8A CN201910441970A CN110075938A CN 110075938 A CN110075938 A CN 110075938A CN 201910441970 A CN201910441970 A CN 201910441970A CN 110075938 A CN110075938 A CN 110075938A
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- Prior art keywords
- sample
- liquid storage
- pipe
- storage pipe
- liquid separation
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- 239000007788 liquid Substances 0.000 title claims abstract description 103
- 238000000926 separation method Methods 0.000 title claims abstract description 39
- 239000012530 fluid Substances 0.000 claims abstract description 26
- 238000000429 assembly Methods 0.000 claims description 11
- 230000000712 assembly Effects 0.000 claims description 11
- 238000012864 cross contamination Methods 0.000 abstract description 3
- 238000011017 operating method Methods 0.000 abstract description 2
- 238000000034 method Methods 0.000 description 15
- 206010036790 Productive cough Diseases 0.000 description 3
- 210000003802 sputum Anatomy 0.000 description 3
- 208000024794 sputum Diseases 0.000 description 3
- 230000010165 autogamy Effects 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 238000005213 imbibition Methods 0.000 description 2
- 244000000010 microbial pathogen Species 0.000 description 2
- 239000000443 aerosol Substances 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000009191 jumping Effects 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012856 packing Methods 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/02—Burettes; Pipettes
- B01L3/021—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/02—Burettes; Pipettes
- B01L3/021—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
- B01L3/0213—Accessories for glass pipettes; Gun-type pipettes, e.g. safety devices, pumps
Landscapes
- Health & Medical Sciences (AREA)
- Clinical Laboratory Science (AREA)
- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The invention discloses a kind of liquid separation sample injectors, for the liquid separation and sample-adding operation in Biohazard Safety Equipment, comprising: liquid storage pipe, for storing pretreatment fluid;It is loaded bar, one end of the sample-adding bar is equipped with the sample-adding piston to match with the liquid storage pipe, and the sample-adding bar is equipped with one end of the sample-adding piston in the sample-adding in-pipe;Pipette, the pipette are connected to the liquid storage pipe, and are loaded into the liquid storage pipe;Sample-adding pipe, the sample-adding pipe are connected to the liquid storage pipe, the pretreatment fluid in the liquid storage pipe are added to specimen container.The liquid separation sample injector of technical solution of the present invention can integrate two kinds of functions of liquid separation and sample-adding, and eliminate previous suction sample link, simplify operating procedure, and the possibility of cross contamination is prevented, improve the accuracy of experimental result, the liquid separation and continuous sample-adding function of quantitative liquid are realized, the different liquid volume addeds between different specimens can be operated, greatly improve working efficiency.
Description
Technical field
The present invention relates to field of experiment equipment more particularly to a kind of liquid separation sample injectors.
Background technique
Biohazard Safety Equipment is medical laboratory's commonly used equipment, and effect is operation containing potential pathogenic microorganism or other danger
Device of the danger because preventing aerosol dissipation when subsample, Clinical microorganism room operate clinical sputum mark using Biohazard Safety Equipment
This when, need to use pretreatment fluid to handle sputum.
There are four types of the sample loading alternatives of conventional pretreatment liquid: (1) using the aliquot treatment fluid of purchase or autogamy, due to sterile behaviour
The needs of work, the aliquot treatment fluid of autogamy need high pressure, sterilize and use after jumping a queue;(2) using treatment fluid bottle topple over sample-adding or
The process for being loaded using wash bottle lid, but toppling over sample-adding can not accurately control sample-adding amount, and Yi Fasheng liquid, which overflows, to be splashed and cause to intersect dirty
It is bothersome laborious to squeeze sample-adding for dye;(3) it is loaded using sample loading gun, due to operating the particularity of pathogenic microorganism in Biohazard Safety Equipment,
It is loaded using traditional sample loading gun, needs constantly to repeat to inhale sample and sample-adding process, and there are gas flowing, sample-addings in safety cabinet
It is cumbersome and easily cause pretreated pollution, to influence the accuracy of experimental result;(4) it is carried out using traditional dispenser
Sample-adding, although common bottleneck dispenser has the characteristics that convenient, fast sample-adding, since Biohazard Safety Equipment space limits,
Heavy, the bad holding in adding mouth position is loaded easily to overflow in common Sputum cup and splash.
Summary of the invention
In view of the defect of the prior art, what technical solution of the present invention solved is the sample loading alternative mistake of existing pretreatment fluid
Journey is cumbersome, the bad control of sample-adding amount, and Yi Fasheng liquid overflows and splashes, and easily causes pretreated pollution, to influence experimental result
Accuracy the technical issues of.
In order to solve the above-mentioned technical problem, technical solution of the present invention provides a kind of liquid separation sample injector, is used for bio-safety
It include: liquid storage pipe in cabinet, for storing pretreatment fluid;It is loaded bar, one end of the sample-adding bar is equipped with and the liquid storage pipe phase
The sample-adding piston matched, the sample-adding bar are equipped with one end of the sample-adding piston in the sample-adding in-pipe;Pipette, it is described
Pipette is connected to the liquid storage pipe, and is loaded into the liquid storage pipe;Sample-adding pipe, the sample-adding pipe connect with the liquid storage pipe
It is logical, the pretreatment fluid in the liquid storage pipe is added to specimen container.
Optionally, the liquid separation sample injector further includes shell, and the liquid storage pipe is located in the shell, the sample-adding
Bar, the pipette and the sample-adding Guan Jun run through the shell.
Optionally, elastomeric element is equipped in the shell, the sample-adding bar is equipped with one end of the sample-adding piston successively
Through the shell, the elastomeric element and protrude into the liquid storage pipe.
Optionally, the liquid separation sample injector further includes dosing assemblies, and the dosing assemblies are including quantitative screw rod and quantitatively
Track groove, the quantitative track groove is set to the liquid storage pipe on the tube wall of nozzle one end and the quantitative track groove is described in
The axial setting of liquid storage pipe, the quantitative screw rod, which runs through, to be sequentially passed through the shell, the quantitative track groove and protrudes into the storage
In liquid pipe, the bar wall of the sample-adding bar is equipped with an obstacle, when the obstacle contacts the quantitative screw rod, the sample-adding bar
Stop movement.
Optionally, the quantitative screw rod tightens fixation by screw thread with the shell, the quantitative track groove.
Optionally, the dosing assemblies further include setting-up piece, and the quantitative screw rod sequentially passes through the setting-up piece, institute
State shell and the quantitative track groove.
Optionally, the liquid separation sample injector further includes moving back pipette tips device, it is described move back pipette tips device include move back pipette tips sleeve,
Move back pipette tips handle and connecting rod, the tube wall for moving back pipette tips sleeve and being sheathed on the sample-adding pipe, it is described move back pipette tips sleeve with it is described
Pipette tips handle is moved back to be connected by the connecting rod.
Optionally, it is equipped with guide groove along the axial direction of the shell, the pipette tips handle that moves back runs through the guide groove simultaneously
It is connected with the connecting rod.
Optionally, the mouthpiece cover of the sample-adding pipe is equipped with disposable pipette tips, and the sample-adding pipe passes through the disposable pipette tips
Pretreatment fluid is added to specimen container.
Optionally, the pipette, be equipped with check valve on the sample-adding pipe.
Compared with prior art, the present invention has following advantageous effects:
The liquid separation sample injector of technical solution of the present invention is by changing sample-adding bar in the position control liquid storage pipe in liquid storage pipe
The sucking and export of pretreatment fluid, pressing sample-adding bar carry out sample-adding operation, and pull is loaded bar and carries out imbibition operation, saves previous
Imbibing step avoids repeating pretreated pollution caused by inhaling sample, improves the accuracy of experimental result.
It is equipped with elastomeric element inside the shell, sample-adding bar sequentially passes through the shell, elastomeric element, when pressing sample-adding bar reaches
Behind predetermined position, load procedure is completed;Sample-adding bar is unclamped, sample-adding bar automatically restores to original position under the action of elastomeric element, together
When inhale sample step complete, realize continuous sample-adding, greatly improve working efficiency.
Dosing assemblies are set, sample-adding amount can be accurately controlled, control fluid trend is equipped on pipette, sample-adding pipe
Check valve, improve liquid separation sample-adding accuracy.
The mouthpiece cover for being loaded pipe is equipped with disposable pipette tips, and the liquid separation sample injector of technical solution of the present invention is enabled to operate band
There is the sample of pollution sources.
Pipette tips device is moved back in setting, when needing replacing disposable pipette tips, need to only press and move back pipette tips handle, so that it may conveniently slough
Disposable pipette tips are pulled out without artificially going.
In conclusion the liquid separation sample injector of technical solution of the present invention can integrate two kinds of functions of liquid separation and sample-adding, and
Previous suction sample link is eliminated, operating procedure is simplified, and prevented the possibility of cross contamination, improves the standard of experimental result
True property realizes the liquid separation and continuous sample-adding function of quantitative liquid, can grasp to the different liquid volume addeds between different specimens
Make, greatly improves working efficiency.
Detailed description of the invention
Fig. 1 is the structural schematic diagram of the liquid separation sample injector of the embodiment of the present invention;
Fig. 2 is the main view of the dosing assemblies of the embodiment of the present invention;
Fig. 3 is the side view of the dosing assemblies of the embodiment of the present invention.
Specific embodiment
As shown in Figure 1, the liquid separation sample injector of the embodiment of the present invention, other than it can be applied in Biohazard Safety Equipment,
It can be applied to other scenes for needing to be loaded.It mainly includes liquid storage pipe 1, sample-adding bar 2, pipette 3 and sample-adding pipe 4.
Wherein, liquid storage pipe 1 is loaded for squeezing pretreatment fluid for storing pretreatment fluid, sample-adding bar 2, is loaded bar 2
One end be equipped with the sample-adding piston 21 that matches with liquid storage pipe 1, sample-adding bar 2 is equipped with one end of sample-adding piston 21 in liquid storage pipe 1
Interior movement is similar to piston motion, and sample-adding piston 21, which squeezes pretreatment fluid, makes it that liquid storage pipe 1 be discharged.It is loaded on bar 2 and lives with sample-adding
It fills in 21 opposite one end and is additionally provided with sample-adding handle 22, facilitate operation.
Pipette 3 is connected to liquid storage pipe 1, when the predetermined position being loaded in the arrival liquid storage pipe 1 of bar 2, the past return of sample-adding bar 2
Dynamic process is equivalent to imbibition process, and pretreatment fluid is entered in liquid storage pipe 1 by pipette 3, completes to inhale sample operation, for next time
Sample-adding is prepared.
Sample-adding pipe 4 is connected to liquid storage pipe 1, and when squeezing the pretreatment fluid in liquid storage pipe 1, pretreatment fluid passes through sample-adding bar 2
Liquid-feeding tube 4 is added to specimen container.
Pipette 3 is equipped with check valve 31, and sample-adding pipe 4 is equipped with check valve 41.When carrying out sample-adding operation, close unidirectional
Valve 31 prevents pretreatment fluid from flowing backward to pipette 3, opens simultaneously check valve 41, passes through the pretreatment fluid being loaded in pipe 4 steadily
Check valve 41 flows to specimen container.When carrying out inhaling sample operation, check valve 41 is closed, prevents the sample-adding liquid in pipette 4 from falling
Stream opens check valve 31 so that sample-adding amount is unable to measure, and pretreatment fluid is made to fill liquid storage pipe 1 again by pipette 3.
Shell 11 is also provided in order to assemble each component well please continue to refer to Fig. 1, wherein liquid storage pipe 1 is located at shell
In 11, sample-adding bar 2, pipette 3 and sample-adding pipe 4 run through shell 11.
Further, elastomeric element 12 is equipped in shell 11, one end that sample-adding bar 2 is equipped with the sample-adding piston is successively passed through
Shell 11, elastomeric element 12 are worn, elastomeric element 12 is, for example, spring.After pressing sample-adding bar 2 reaches predetermined position, sample-adding is unclamped
Bar 2, under the action of elastomeric element 12, sample-adding bar 2 is automatically moved back to original position, and the process that sample-adding bar 2 sets back is completed
It is primary to inhale sample step, eliminate previous cumbersome suction sample step.
In addition, can also set hook 14 on shell 11, the suspension of liquid separation sample injector can be made to be placed in Biohazard Safety Equipment, saved
Space.
In conjunction with shown in Fig. 2 and Fig. 3, for the precision for improving sample-adding with dividing sample, the liquid separation sample injector of the embodiment of the present invention is also
Equipped with dosing assemblies, dosing assemblies include quantitative screw rod 6 and quantitative track groove 7, and quantitative track groove 7 is set to liquid storage pipe 1 close to pipe
On the tube wall 11 of mouth one end, quantitative track groove 7 is along the axial direction setting of liquid storage pipe 1, and the length of quantitative track groove 7 is according to the actual situation
Setting.Quantitative screw rod 6 sequentially passes through shell 11, quantitative track groove 7 and protrudes into liquid storage pipe 1, and the bar wall of sample-adding bar 2 is equipped with one
Obstacle 8, when sample-adding bar 2, which is moved to obstacle 8, contacts quantitative screw rod 6, sample-adding bar 2 stops moving, and completes sample-adding process.
It is to be removably secured between quantitative screw rod 6 and shell 11, quantitative track groove 7.Specifically, have on quantitative screw rod 6
There is screw thread, is respectively equipped with the screw thread to match with screw rod 6 on shell 11, quantitative track groove 7, shell 11 tightens fixation by screw thread
On shell 11 and quantitative track groove 7.Further, it is additionally provided with quantitative scale on quantitative track groove 7, it, can before load procedure
According to scheduled sample-adding amount, tightened at corresponding graduation position in mobile quantitative screw rod 6 to quantitative track groove 7.When sample-adding, add
The piston 21 of 2 one end of sample bar moves in liquid storage pipe 1, until the obstacle 8 on sample-adding bar 2 is contacted with quantitative screw rod 6.At this point,
Bar 2 is loaded due to that can not be continued to move to by the resistance of quantitative screw rod 6, illustrates to have reached sample-adding amount, sample-adding process terminates.
It is preferably fixed on shell 11 and quantitative track groove 7 for the ease of quantitative screw rod 6, is additionally provided with setting-up piece
10, quantitative screw rod 6 sequentially passes through setting-up piece 10, shell 11 and quantitative track groove 7.
Please continue to refer to Fig. 1, in the present embodiment, the mouthpiece cover of sample-adding pipe 4 is equipped with disposable pipette tips 5, and sample-adding pipe 4 passes through
For disposable pipette tips 5 by pretreatment fluid liquid separation to specimen container, disposable pipette tips 5 are sheathed on the nozzle of sample-adding pipe 4.
In the present embodiment, liquid separation sample injector, which is additionally provided with, moves back pipette tips device.After the completion of sample-adding, moving back pipette tips device can will be primary
Property pipette tips 5 sloughed from sample-adding pipe 4, prepare for sample-adding next time.The pipette tips device that moves back of the embodiment of the present invention includes
It moves back pipette tips sleeve 91, move back pipette tips handle 92 and connecting rod 93, move back pipette tips sleeve 91 and be sheathed on sample-adding pipe 4, move back pipette tips sleeve 91
It is connected with pipette tips handle 92 is moved back by connecting rod 93.
Axial direction along shell 11 is equipped with guide groove 13, and the length of guide groove 13 is not required, sets according to the actual situation
Meter.92 one end of pipette tips handle is moved back through guide groove 13 and is connected with connecting rod 93.
Fig. 1 is please referred to, moving back pipette tips handle 92 can move along the length direction of guide groove 13, when load procedure is completed to need
When sloughing disposable pipette tips 5, it only need to will move back pipette tips handle 92 and be moved i.e. along guide groove 13 to the direction that disposable pipette tips 5 are sloughed
Can, Fig. 1 has illustratively indicated the moving direction for moving back pipette tips handle 92.
The working principle of the liquid separation sample injector of the embodiment of the present invention is further described below, to better understand
Technical solution of the present invention.
Pretreatment fluid is filled in liquid storage pipe 1, and quantitative screw rod 6 is moved on quantitative track groove 7 at predetermined graduated and is twisted
It fastens, palm holds continuous liquid separation sample injector, pressing sample-adding bar handle 22, and check valve 41 is opened, and check valve 31 is closed, sample-adding
Axial direction of the bar 2 in liquid storage pipe 1 along liquid storage pipe 1 moves, and liquid is pressed into sample-adding pipe 4 in liquid storage pipe 1, and flow direction is primary
Property pipette tips 5, and then be added in specimen container and carry out sample-adding operation, until the obstacle 8 on sample-adding bar 2 contacts quantitative screw rod 6,
Illustrate to have arrived at scheduled sample-adding amount, sample-adding bar 2 stops movement, and sample-adding operation terminates.
Then, it carries out inhaling sample operation into liquid storage pipe 1.The pressure to sample-adding handle 22 is removed, check valve 31 is opened, unidirectionally
Valve 41 is closed, and sample-adding bar 2 restores under the action of elastomeric element 12 to original position, and liquid fills liquid storage pipe 1 again, is completed
Inhale sample process.
After sample-adding process, carry out moving back pipette tips operation.Pipette tips handle is moved back in the direction pressing being detached to disposable pipette tips 5
92, the nozzle that disposable pipette tips 5 are sheathed on sample-adding pipe 4 is squeezed with the pipette tips sleeve 91 that moves back that pipette tips 92 are connect is moved back, until disposable
Pipette tips 5 are taken off, and moving back pipette tips operation terminates, and complete a wheel liquid separation and are loaded process.
In conclusion the liquid separation sample injector of technical solution of the present invention is simplified in Biohazard Safety Equipment and is pre-processed to sample
The process of liquid sample-adding reduces because of operation bring cross contamination, realizes continuous sample-adding, greatly improve working efficiency,
Controllable sample-adding amount function can operate the different sample-adding amounts between different specimens, and structure is simple, light and handy, is able to carry out
Hand held sample-adding, for precisely sample-adding provides the foundation in Biohazard Safety Equipment.Certainly, the liquid separation sample injector of technical solution of the present invention
It still can be used outside Biohazard Safety Equipment, such as packing and the sample-adding of small volumes of liquids.
The preferred embodiment of the present invention has been described in detail above.It should be appreciated that those skilled in the art without
It needs creative work according to the present invention can conceive and makes many modifications and variations.Therefore, all technologies in the art
Personnel are available by logical analysis, reasoning, or a limited experiment on the basis of existing technology under this invention's idea
Technical solution, all should be within the scope of protection determined by the claims.
Claims (10)
1. a kind of liquid separation sample injector, in Biohazard Safety Equipment characterized by comprising
Liquid storage pipe, for storing pretreatment fluid;
It is loaded bar, one end of the sample-adding bar is equipped with the sample-adding piston to match with the liquid storage pipe, and the sample-adding bar is equipped with
One end of the sample-adding piston is in the sample-adding in-pipe;
Pipette, the pipette are connected to the liquid storage pipe, and are loaded into the liquid storage pipe;
Sample-adding pipe, the sample-adding pipe are connected to the liquid storage pipe, the pretreatment fluid in the liquid storage pipe are added to specimen container.
2. liquid separation sample injector as described in claim 1, which is characterized in that further include shell, the liquid storage pipe is located at described outer
In shell, the sample-adding bar, the pipette and the sample-adding Guan Jun run through the shell.
3. liquid separation sample injector as claimed in claim 2, which is characterized in that be equipped with elastomeric element, the sample-adding in the shell
One end that bar is equipped with the sample-adding piston sequentially passes through the shell, the elastomeric element and protrudes into the liquid storage pipe.
4. liquid separation sample injector as claimed in claim 2, which is characterized in that further include dosing assemblies, the dosing assemblies include
Quantitative screw rod and quantitative track groove, it is on the tube wall of nozzle one end and described fixed that the quantitative track groove is set to the liquid storage pipe
Axial direction setting of the track groove along the liquid storage pipe is measured, the quantitative screw rod sequentially passes through the shell, the quantitative track groove simultaneously
It protruding into the liquid storage pipe, the bar wall of the sample-adding bar is equipped with an obstacle, when the obstacle contacts the quantitative screw rod,
The sample-adding bar stops movement.
5. liquid separation sample injector as claimed in claim 4, which is characterized in that the quantitative screw rod and the shell, described quantitative
Track groove tightens fixation by screw thread.
6. liquid separation sample injector as claimed in claim 5, which is characterized in that the dosing assemblies further include setting-up piece, described
Quantitative screw rod sequentially passes through the setting-up piece, the shell and the quantitative track groove.
7. liquid separation sample injector as claimed in claim 2, which is characterized in that it further include moving back pipette tips device, it is described to move back pipette tips device
Including moving back pipette tips sleeve, moving back pipette tips handle and connecting rod, the tube wall for moving back pipette tips sleeve and being sheathed on the sample-adding pipe is described to move back
Pipette tips sleeve moves back pipette tips handle and is connected by the connecting rod with described.
8. liquid separation sample injector as claimed in claim 7, which is characterized in that the axial direction along the shell is equipped with guide groove,
It is described to move back pipette tips handle through the guide groove and be connected with the connecting rod.
9. liquid separation sample injector as described in claim 1, which is characterized in that the mouthpiece cover of the sample-adding pipe is equipped with disposable rifle
Pretreatment fluid is added to specimen container by the disposable pipette tips for head, the sample-adding pipe.
10. liquid separation sample injector as described in claim 1, which is characterized in that be equipped with list in the pipette, the sample-adding pipe
To valve.
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CN201910441970.8A CN110075938A (en) | 2019-05-24 | 2019-05-24 | A kind of liquid separation sample injector |
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CN201910441970.8A CN110075938A (en) | 2019-05-24 | 2019-05-24 | A kind of liquid separation sample injector |
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CN110075938A true CN110075938A (en) | 2019-08-02 |
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CN201910441970.8A Pending CN110075938A (en) | 2019-05-24 | 2019-05-24 | A kind of liquid separation sample injector |
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Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN111505314A (en) * | 2020-05-07 | 2020-08-07 | 厦门万渤生物技术有限公司 | Novel combined detection kit for coronavirus antibody and application thereof |
CN111505313A (en) * | 2020-05-07 | 2020-08-07 | 厦门万渤生物技术有限公司 | Diagnostic kit for combined diagnosis of mycoplasma pneumonia and application thereof |
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CN206479366U (en) * | 2017-02-17 | 2017-09-08 | 中国石油化工股份有限公司 | High pressure water-injection pump underground fuel tank lubricating oil sampling instrument |
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CN210115079U (en) * | 2019-05-24 | 2020-02-28 | 上海市第五人民医院 | Liquid separation sample injector |
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CA2253754A1 (en) * | 1996-05-06 | 1997-11-13 | Daniel G. Cheresko | Oil and gas metering and measuring device |
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CN111505314B (en) * | 2020-05-07 | 2022-07-05 | 厦门万渤生物技术有限公司 | Novel combined detection kit for coronavirus antibody and application thereof |
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