CN110074134A - Foamaseptic of coccidian oocyst and preparation method thereof is killed in a kind of suppression - Google Patents
Foamaseptic of coccidian oocyst and preparation method thereof is killed in a kind of suppression Download PDFInfo
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- CN110074134A CN110074134A CN201910486763.4A CN201910486763A CN110074134A CN 110074134 A CN110074134 A CN 110074134A CN 201910486763 A CN201910486763 A CN 201910486763A CN 110074134 A CN110074134 A CN 110074134A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/02—Saturated carboxylic acids or thio analogues thereof; Derivatives thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
Abstract
The present invention relates to disinfectant fields, specifically foamaseptic of coccidian oocyst and preparation method thereof is killed in a kind of suppression, provide not only a kind of component for pressing down and killing the foamaseptic of coccidian oocyst, be made of the raw material of following mass percent: sodium nitrite 0.103%~0.41%, glacial acetic acid 0.18%~1.44%, foaming agent 0.1%~4%, stabilizer 0.1%~4%, surplus are water；The present invention also provides the preparation methods that the foamaseptic of coccidian oocyst is killed in suppression.The coccidian oocyst disinfectant that the present invention develops can efficiently press down the activity of kill worm unsporulated oocysts and Sporulated Oocysts, and no cause cytogene mutation effect is a kind of efficient, safe and environmentally friendly coccidian oocyst environment disinfectant to animal house cage tool without obvious corrosivity.
Description
Technical field
The present invention relates to disinfectant technical fields, specifically a kind of to press down the foamaseptic and its system for killing coccidian oocyst
Preparation Method.
Background technique
Globidiosis has infection rate height and pathogenic strong characteristic, thus to farms such as chicken house, Ya Chang, warren and pig farms
Serious harm is generated, wherein the death rate of young animal is up to 80% or more.
The animal's coccidiosis prevention and treatment of the prior art relies primarily on coccidiostat, and with the long-time service of coccidiostat
And abuse, significant coccidia drug resistance problems are had resulted at present.Therefore, screening a kind of can effectively kill disappearing for coccidian oocyst
Toxic agent is most important to farm's control globidiosis.
Coccidian oocyst includes unsporulated oocysts and Sporulated Oocysts, and wherein there are tough and tensile egg capsules for unsporulated oocysts
Wall, for Sporulated Oocysts there are tough and tensile egg capsule wall and spore cyst wall, tough and tensile egg capsule wall keeps coccidian oocyst with super strength anti-dry
The ability of dry and anti-chemosterilant infiltration, conventional chemical disinfectant for example sodium hydroxide, Peracetic acid, glutaraldehyde, benzalkonium bromide,
Active silver ion solution etc. cannot kill Sporulated Oocysts.There is presently no the coccidian oocyst disinfectant of effective commercialization,
Therefore develop it is a kind of can efficiently press down kill coccidian oocyst foamaseptic it is very necessary.
We study Eimeria species with Infection in Vitro test in vivo, and result of study is shown, IFN-γ activates huge
The NO that phagocyte generates can effectively kill the coccidia polypide in target cell, by the inspiration of this important research result, it is presumed that outside
Property NO in source also can equally have effects that suppression is killed to coccidian oocyst, and based on the Immunology of above-mentioned coccidium infection, the present invention is caused
Power a kind of can efficiently press down the foamaseptic for killing coccidian oocyst, and safe and environment-friendly in developing.
Summary of the invention
For technical deficiency of the existing technology, the object of the present invention is to provide a kind of foams that coccidian oocyst is killed in suppression to disappear
Toxic agent and preparation method thereof, the coccidian oocyst disinfectant that the present invention develops can efficiently press down kill worm unsporulated oocysts and Sporulated
The activity of egg capsule, no cause cytogene mutation effect are efficient one kind, safety and environmental protection to animal house cage tool without obvious corrosivity
Coccidian oocyst environment disinfectant.
In order to solve the above technical problems, the present invention adopts the following technical scheme:
It is a kind of to press down the foamaseptic for killing coccidian oocyst, it is made of the raw material of following mass percent: sodium nitrite
0.103%~0.41%, glacial acetic acid 0.18%~1.44%, foaming agent 0.1%~4%, stabilizer 0.1%~4%, water
90.51~99.517%.
Preferably, the mass percent of the foaming agent is 0.32%~0.64%, and the foaming agent is dodecyl two
Methylbenzyl ammonium chloride.
Preferably, the mass percent of the foaming agent is 0.1%~3%, and the foaming agent is hydroxyethyl cellulose.
Preferably, the mass percent of the foaming agent is 0.5%~4%, and the foaming agent is dodecyl benzene sulfonic acid
Sodium.
Preferably, the mass percent of the stabilizer is 0.1%~1%, and the stabilizer is Arabic gum.
Preferably, the stabilizer is xanthan gum.
The present invention also protects a kind of preparation method for pressing down and killing the foamaseptic of coccidian oocyst, includes the following steps:
(1) each raw material is weighed according to following mass percent: 0.103%~0.41% sodium nitrite, 0.18%~
1.44% glacial acetic acid, 0.1%~4% foaming agent, 0.1%~4% stabilizer, 90.51~99.517% water are spare；
(2) the weighed glacial acetic acid of step (1), foaming agent and stabilizer are uniformly mixed, obtain solution A；Step (1) is claimed
The sodium nitrite taken is uniformly mixed with water, obtains solution B；
(3) solution A and the solution B mixing obtained step (2), stirs evenly, and obtains suppression and kill the foam of coccidian oocyst disappearing
Toxic agent.
Preferably, the revolving speed of step (3) described stirring is 1000-1500r/min, mixing time 3-5min.
Compared with prior art, the invention has the advantages that:
1, the glacial acetic acid in the present invention can react in the state that suitable proportion mixes with sodium nitrite, generate
NO, NO can effectively be penetrated into inside coccidia unsporulated oocysts under weak acid environment, effectively inhibit egg capsule in plasm into
The division of one step loses unsporulated oocysts and divides again so that the Sporulated process to unsporulated oocysts generates inhibiting effect
Ability；NO also can effectively pass through the egg capsule wall and spore cyst wall of Sporulated Oocysts simultaneously, directly contact with zygoblast, performance is killed
Hurt zygoblast effect, Sporulated Oocysts is caused to lose infection activity.
2, in the present invention foaming agent and stabilizer to be capable of forming most after being mixed in the case of optimal proportion provided by the invention
Large volume and the foam for continuing 1 hour or more, under the mediation of foam, when coccidian oocyst contacts in antimicrobial activity ingredient and environment
Between it is longer, to kill effect more thorough for suppression；Foam can be attached to the body surfaces such as ground, metope and the cage tool of animal house simultaneously, reach
Sterilize the purpose without dead angle；In addition compared with non-foam disinfectant, the dosage of disinfectant and water can be greatlyd save.
3, the present invention is environment disinfectant, and animal is evicted out of cultured space when in use, and by cultured space it is closed after
Spray disinfectant, due to containing foam in thimerosal, after NO generation, NO a part is located in foam, a part attaching
In on foam, NO is easily acted on coccidian oocyst, therefore a large amount of NO is absorbed by coccidian oocyst, and remaining NO is oxidized and and water
Reaction generates nitric acid, and since glacial acetic acid is weak acid, and dosage is few, thus glacial acetic acid reacted with sodium nitrite generation NO it is few,
The amount for the nitric acid for being so oxidized and generating after reacting with water is quite few, also just does not cause to corrode to environment.
4, the present invention is through mouse lymphoma cell TK gene mutation test the results show that the coccidian oocyst foam-type disinfectant
To the TK gene of L5178Y cell without mutagenesis, security performance is strong.
5, the present invention by metal protection test the result shows that: be that 5% glacial acetic acid+3.6% is sub- in mass percent
Working concentration (5 times of work of the thimerosal of+0.4% Arabic gum of+2.4% dodecyl benzyl dimethyl ammonium chloride of sodium nitrate
Concentration) under, for the disinfectant to carbon steel, stainless steel, aluminium and copper common metal without obvious corrosivity, application space is extensive.
Specific embodiment
Below with reference to 1- of embodiment of the present invention embodiment 43 and comparative example 1- comparative example 13, carried out to preferred embodiment
Detailed description.
Embodiment 1
The present embodiment provides the foamaseptics that coccidian oocyst is killed in a kind of suppression, are made of the raw material of following mass percent:
Sodium nitrite 0.103%, glacial acetic acid 0.72%, dodecyl benzyl dimethyl ammonium chloride 0.48%, Arabic gum 0.3%, it is remaining
Amount is water；
The present embodiment also provides the preparation method that the foamaseptic of coccidian oocyst is killed in above-mentioned suppression, and steps are as follows:
(1) according to the mass percent in the formula of the present embodiment, glacial acetic acid, Arabic gum and dimethyl are measured
Base benzyl ammonium chloride, sodium nitrite and water, it is spare；
(2) the weighed glacial acetic acid of step (1), Arabic gum and dodecyl benzyl dimethyl ammonium chloride are uniformly mixed,
Obtain solution A；Sodium nitrite and water are mixed to get solution B；
(3) solution B of the solution A of step (2) and step (2) is stirred into 3min, shape under the mixing speed of 1000r/min
At foam disinfectant liquid.
Embodiment 2
Specific preparation process is identical as the process of embodiment 1, the difference is that only, the mass percent of sodium nitrite is
0.136%；
Embodiment 3
Specific preparation process is identical as the process of embodiment 1, the difference is that only, the mass percent of sodium nitrite is
0.206%；
Embodiment 4
Specific preparation process is identical as the process of embodiment 1, the difference is that only, the mass percent of sodium nitrite is
0.272%；
Embodiment 5
Specific preparation process is identical as the process of embodiment 1, the difference is that only, the mass percent of sodium nitrite is
0.34%；
Embodiment 6
Specific preparation process is identical as the process of embodiment 1, the difference is that only, the difference is that the quality of sodium nitrite
Percentage is 0.41%；
Comparative example 1
Tap water used in embodiment 1- embodiment 7.
Comparative example 2
Sodium nitrite is dissolved in water, obtains the sodium nitrite solution that 100mL mass percent is 0.41%.
Comparative example 3
Specific preparation process is identical as the process of embodiment 1, the difference is that only, the mass percent of sodium nitrite is
0.0068%；
The sample of embodiment 1- embodiment 6 and comparative example 1- comparative example 3 has been carried out non-Sporulated suppression and has killed effect by us
Experiment, that is, the sample of embodiment 1- embodiment 6 and comparative example 1- comparative example 3 is fresh with Eimeria tenella respectively
Unsporulated oocysts act on 2h.
Sporulated inhibitory effect of 1 thimerosal of table to non-Sporulated coccidian oocyst
Above-described embodiment 1- embodiment 6 is the research optimized to mass percent shared by sodium nitrite, to choose
Mass percent shared by sodium nitrite under the Sporulated inhibitory effect optimal situation of non-Sporulated coccidian oocyst, in order to optimize
Glacial acetic acid mass percent in solution A is set as 0.72%, dodecyl by mass percent shared by sodium nitrite in solution B
Dimethyl benzyl ammonium chloride mass percent is set as 0.48%, and Arabic gum mass percent is set as 0.3%, nitrous in solution B
Sour sodium mass percent is respectively set to 0.103%, 0.136%, 0.206%, 0.272%, 0.34% and 0.41% totally 6 matter
The gradient of percentage is measured, it is fresh with Eimeria tenella after solution A is mixed with the solution B of different quality percentage respectively
Unsporulated oocysts act on 2h.Setting water treatment group (comparative example 1) contains only 0.41% sodium nitrite processing group (i.e. only
Have solution B, comparative example 2) and sodium nitrite mass percent be 0.0068% thimerosal processing group (comparative example 3) as pair
According to group, after Sporulated culture, microscopically observation and calculating egg capsule Sporulated rate.The results are shown in Table 1, is containing mass percent
The thimerosal of 0.103%~0.41% sodium nitrite is presented 100% to the Sporulated process of unsporulated oocysts and inhibits, individually
0.41% sodium nitrite processing group (solution A, comparative example 2 is not added) and water treatment group (comparative example 1) are to non-Sporulated
The Sporulated process of egg capsule does not have inhibiting effect, and the thimerosal processing group that the mass percent of sodium nitrite is 0.0068% is (right
Ratio 3) there is partial inhibition, therefore obtain: to non-spore when the mass percent of sodium nitrite is 0.103%~0.41%
The Sporulated for changing coccidian oocyst inhibits effect preferable.
Embodiment 7
The present embodiment provides the foamaseptics that coccidian oocyst is killed in a kind of suppression, are made of the raw material of following mass percent:
Sodium nitrite 0.206%, glacial acetic acid 0.18%, dodecyl benzyl dimethyl ammonium chloride 0.48%, Arabic gum 0.3%, it is remaining
Amount is water；
The present embodiment also provides the preparation method that the foamaseptic of coccidian oocyst is killed in above-mentioned suppression, and steps are as follows:
(1) according to the mass percent in the formula of the present embodiment, glacial acetic acid, Arabic gum and dimethyl are measured
Base benzyl ammonium chloride, sodium nitrite and water, it is spare；
(2) the weighed glacial acetic acid of step (1), Arabic gum and dodecyl benzyl dimethyl ammonium chloride are uniformly mixed,
Obtain solution A；Sodium nitrite and water are mixed to get solution B；
(3) solution B of the solution A of step (2) and step (2) is stirred into 3min, shape under the mixing speed of 1000r/min
At foam disinfectant liquid.
Embodiment 8
Specific preparation process is identical as the process of embodiment 7, the difference is that only, glacial acetic acid mass percent
0.36%；
Embodiment 9
Specific preparation process is identical as the process of embodiment 7, the difference is that only, glacial acetic acid mass percent
0.72%；
Embodiment 10
Specific preparation process is identical as the process of embodiment 7, the difference is that only, glacial acetic acid mass percent
1.08%；
Embodiment 11
Specific preparation process is identical as the process of embodiment 7, the difference is that only, glacial acetic acid mass percent
1.44%；
Comparative example 4
Glacial acetic acid is dissolved in water, obtains the glacial acetic acid solution that 100mL mass percent is 1.44%.
Comparative example 5
Specific preparation process is identical as the process of embodiment 7, the difference is that only, glacial acetic acid mass percent
0.036%；
Comparative example 6
Specific preparation process is identical as the process of embodiment 7, the difference is that only, glacial acetic acid mass percent
0.09%；
The sample of embodiment 7- embodiment 11 and comparative example 1 and comparative example 4- comparative example 6 has been carried out non-Sporulated by us
The experiment of effect is killed in suppression, that is, by the sample of embodiment 7- embodiment 11 and comparative example 1 and comparative example 4- comparative example 6 respectively with
The fresh unsporulated oocysts of Eimeria tenella act on 2h.
The thimerosal of 2 glacial acetic acid of percentage containing different quality of table kills effect to the suppression of the non-Sporulated rate of coccidian oocyst
Above-described embodiment 7- embodiment 11 is optimized to mass percent shared by glacial acetic acid, to choose non-Sporulated
Mass percent shared by glacial acetic acid under the Sporulated inhibitory effect optimal situation of coccidian oocyst, in order to optimize ice in solution A
Dodecyl benzyl dimethyl ammonium chloride mass percent in solution A is set as 0.48% by mass percent shared by acetic acid, Ah
Primary glue mass percent is drawn to be set as 0.3%, sodium nitrite mass percent is set as 0.206% in solution B；Glacial acetic acid in solution A
Mass percent is respectively set to 0.18%, 0.36%, 0.72%, 1.08% and 1.44% totally 5 gradients, solution B respectively with
After solution A mixing containing different glacial acetic acid mass percents, 2h is acted on the fresh unsporulated oocysts of Eimeria tenella.
In addition, setting water treatment group (comparative example 1), independent mass percent are 1.44% glacial acetic acid group (comparative example 4), glacial acetic acid
Mass percent be 0.036% thimerosal (comparative example 5) and glacial acetic acid mass percent be 0.09% thimerosal it is (right
Ratio 6) as a control group, and after Sporulated culture, microscopically observation and calculating egg capsule Sporulated rate.As a result such as 2 institute of table
Show, is in the Sporulated process of coccidia unsporulated oocysts containing the thimerosal that mass percent is 0.18%~1.44% glacial acetic acid
Existing 100% inhibits, only by using 1.44% glacial acetic acid to the Sporulated processes of unsporulated oocysts substantially without inhibiting effect,
The mass percent of thimerosal (comparative example 5) and glacial acetic acid that the mass percent of glacial acetic acid is 0.036% is 0.09% to disappear
Venom (comparative example 6) has partial inhibition, therefore obtains: suppression is killed when the mass percent of glacial acetic acid is 0.18%~1.44%
Effect is preferable.
Embodiment 12
It is identical as the process of embodiment 9, it the difference is that only, foam disinfectant liquid and Eimeria tenella are fresh not
Sporulated Oocysts action time is 60min；
Embodiment 13
It is identical as the process of embodiment 9, it the difference is that only, foam disinfectant liquid and Eimeria tenella are fresh not
Sporulated Oocysts action time is 120min；
Embodiment 14
It is identical as the process of embodiment 9, it the difference is that only, foam disinfectant liquid and Eimeria tenella are fresh not
Sporulated Oocysts action time is 180min；
Embodiment 15
It is identical as the process of embodiment 9, it the difference is that only, foam disinfectant liquid and Eimeria tenella are fresh not
Sporulated Oocysts action time is 240min；
Comparative example 7
It is identical as the process of embodiment 9, it the difference is that only, foam disinfectant liquid and Eimeria tenella are fresh not
Sporulated Oocysts action time is 15min；
Comparative example 8
It is identical as the process of embodiment 9, it the difference is that only, foam disinfectant liquid and Eimeria tenella are fresh not
Sporulated Oocysts action time is 30min；
The sample of embodiment 12- embodiment 15 and comparative example 1 and comparative example 7- comparative example 8 has been carried out non-Sporulated by us
The experiment of effect is killed in suppression, is killed action time under effect optimal situation to choose suppression, that is, by embodiment 12- embodiment 15
Unsporulated oocysts fresh with Eimeria tenella are made respectively respectively with the sample of comparative example 1 and comparative example 7- comparative example 8
With 15min, 30min, 60min, 120min, 180min and 240min.
The optimum results of table 3 thimerosal and unsporulated oocysts action time
Above-described embodiment 12- embodiment 15 is the optimizing research of thimerosal Yu non-Sporulated coccidian oocyst action time, with
Phase chooses the action time pressed down and killed under effect optimal situation, in order to optimize thimerosal and unsporulated oocysts action time, solution A
Middle glacial acetic acid mass percent is set as 0.72%, and dodecyl benzyl dimethyl ammonium chloride mass percent is set as 0.48%, Ah
Primary glue mass percent is drawn to be set as 0.3%, sodium nitrite mass percent is set as 0.206% in solution B.Solution A and solution B are mixed
After conjunction, the unsporulated oocysts action time fresh with Eimeria tenella be respectively set 60min, 120min, 180min and
240min totally 4 time points, in addition, setting act on 240 minutes water treatment group (embodiment 1), foam disinfectant liquid with it is soft
It is respectively 15min (comparative example 7) and 30min (comparative example that the fresh unsporulated oocysts of tender Eimeria, which distinguish action time,
8) as a control group, after Sporulated culture, microscopically observation and calculating egg capsule Sporulated rate.The results are shown in Table 3, thimerosal
To coccidia unsporulated oocysts action time 60min~240min, 100% is presented to its Sporulated process and is inhibited, effect
The water treatment group (embodiment 1) of 240min is to Sporulated process substantially without inhibiting effect, foam disinfectant liquid and tender Chinese mugwort
It is respectively 15min (comparative example 7) and 30min (comparative example 8) right that the fresh unsporulated oocysts of U.S. ear ball worm, which distinguish action time,
Sporulated process has partial inhibition, therefore obtains: pressing down the action time killed under effect optimal situation is 60 minutes or more.
Embodiment 16
It is identical as the process of embodiment 13, it the difference is that only, foam disinfectant liquid and Eimeria tenella Sporulated
Egg capsule effect；
Embodiment 17
It is identical as the process of embodiment 14, it the difference is that only, foam disinfectant liquid and Eimeria tenella Sporulated
Egg capsule effect；
Embodiment 18
It is identical as the process of embodiment 15, it the difference is that only, foam disinfectant liquid and Eimeria tenella Sporulated
Egg capsule effect；
Comparative example 9
It is uninfected by the egg capsule of coccidia；
Comparative example 10
The Sporulated Oocysts infected group of non-sterile liquid processing；
Comparative example 11
It is identical as the process of embodiment 12, it the difference is that only, foam disinfectant liquid and Eimeria tenella Sporulated
Egg capsule effect；
The sample of embodiment 16- embodiment 18 and comparative example 9- comparative example 11 has been carried out Sporulated Oocysts infection and lived by us
Property suppression kill the experiment of effect, kill action time under effect optimal situation to choose suppression, that is, embodiment 16- is implemented
Sporulated Oocysts fresh with Eimeria tenella act on the sample of example 18 and comparative example 9- comparative example 11 respectively respectively
120min, 180min, 240min and 60min, while being compared by blank control group and red control group.
4 thimerosal of table kills effect to the suppression of Sporulated Oocysts infection activity
Note: blank control group: not infected group；Red control group: Sporulated Oocysts infected group is not handled；+: there is bloody stool, ++
+: serious bloody stool；-: excrement is normal, no bloody stool.
18 thimerosal of above-described embodiment 16- embodiment kills the experiment of effect to the suppression of coccidia Sporulated Oocysts Sporulated process
Research, kills effect, disinfection formula of liquid of the invention to observe thimerosal of the present invention to the suppression of the infection activity of Sporulated Oocysts
120min, 180min and 240min totally 3 time points, while setting two are acted on Eimeria tenella Sporulated Oocysts respectively
Group control group, wherein blank control group is the egg capsule (comparative example 9) for being uninfected by coccidia, and red control group is the processing of non-sterile liquid
Sporulated Oocysts infected group (comparative example 10) and disinfection formula of liquid and Eimeria tenella Sporulated Oocysts effect 60min are (right
Ratio 11).The results are shown in Table 4, and serious hematochezia occurs in the Sporulated Oocysts infected group (comparative example 10) of non-sterile liquid processing
The case where, in addition, disinfection formula of liquid and Eimeria tenella Sporulated Oocysts effect 60min (comparative example 11) have hematochezia, and
Do not occur hematochezia situation after effect 2 hours or more, therefore we obtain: thimerosal and Sporulated Oocysts act on 2h or more, can
Effectively press down the infection activity for killing Sporulated Oocysts.
Embodiment 19
It is identical as the process of embodiment 9, it the difference is that only, the quality percentage of dodecyl benzyl dimethyl ammonium chloride
Than being 0.32%；
Embodiment 20
It is identical as the process of embodiment 9, it the difference is that only, the quality percentage of dodecyl benzyl dimethyl ammonium chloride
Than being 0.64%；
Embodiment 21
It is identical as the process of embodiment 19, it the difference is that only, the dodecyl two for being 0.32% by mass percent
Methylbenzyl ammonium chloride replaces with the hydroxyethyl cellulose that mass percent is 0.1%；
Embodiment 22
It is identical as the process of embodiment 19, it the difference is that only, the dodecyl two for being 0.32% by mass percent
Methylbenzyl ammonium chloride replaces with the hydroxyethyl cellulose that mass percent is 0.5%；
Embodiment 23
It is identical as the process of embodiment 19, it the difference is that only, the dodecyl two for being 0.32% by mass percent
Methylbenzyl ammonium chloride replaces with the hydroxyethyl cellulose that mass percent is 1%；
Embodiment 24
It is identical as the process of embodiment 19, it the difference is that only, the dodecyl two for being 0.32% by mass percent
Methylbenzyl ammonium chloride replaces with the hydroxyethyl cellulose that mass percent is 2%；
Embodiment 25
It is identical as the process of embodiment 19, it the difference is that only, the dodecyl two for being 0.32% by mass percent
Methylbenzyl ammonium chloride replaces with the hydroxyethyl cellulose that mass percent is 3%；
Embodiment 26
It is identical as the process of embodiment 19, it the difference is that only, the dodecyl two for being 0.32% by mass percent
Methylbenzyl ammonium chloride replaces with the neopelex that mass percent is 0.5%；
Embodiment 27
It is identical as the process of embodiment 19, it the difference is that only, the dodecyl two for being 0.32% by mass percent
Methylbenzyl ammonium chloride replaces with the neopelex that mass percent is 1%；
Embodiment 28
It is identical as the process of embodiment 19, it the difference is that only, the dodecyl two for being 0.32% by mass percent
Methylbenzyl ammonium chloride replaces with the neopelex that mass percent is 2%；
Embodiment 29
It is identical as the process of embodiment 19, it the difference is that only, the dodecyl two for being 0.32% by mass percent
Methylbenzyl ammonium chloride replaces with the neopelex that mass percent is 4%.
The optimizing research of embodiment 12 and embodiment 19-29 to the mass percent of foaming agent kills effect to choose suppression
The mass percent of foaming agent and different foaming agents under fruit optimal situation, in order to screen suitable blowing agent and optimization foaming agent
Best in quality percentage, sodium nitrite mass percent is set as 0.206% in solution B, glacial acetic acid mass percent in solution A
It is set as 0.72%, Arabic gum mass percent is set as 0.3%, and the solution A containing different foaming agent mass percents and solution B are mixed
After closing constant volume 100mL, the revolving speed of 1000r/min stirs 3min, and measurement generates barmy volume and foam is held time, wherein
Embodiment 12, embodiment 19- embodiment 20 are compared, the mass percent of dodecyl benzyl dimethyl ammonium chloride
0.32%, 0.48%, 0.64% totally 3 gradients are respectively set, the results show that foam volume changes with foaming agents content and become
Change, it is 534mL that when dodecyl benzyl dimethyl ammonium chloride mass percent is 0.48%, the foam volume of generation is maximum.
Above-described embodiment 21- embodiment 25 is compared, wherein hydroxyethyl cellulose mass percent is arranged
0.1%, 0.5%, 1%, 2% and 3% totally 5 gradients, the results show that when hydroxyethyl cellulose mass percent is 2%, foam
Volume is maximum, is 375mL；
Above-described embodiment 26- embodiment 29 is compared, wherein neopelex mass percent is arranged
0.5%, 1%, 2% and 4% totally 4 gradients, the results show that when neopelex mass percent is 2%, foams
Product is maximum, is 446mL；
It is dodecyl dimethyl that mass percent is 0.48% that three kinds of foaming agents, which generate foams and hold time longest,
Benzyl ammonium chloride, foam average duration 42min.
Embodiment 30
It is identical as the process of embodiment 9, it the difference is that only, the mass percent of Arabic gum is 0.1%；
Embodiment 31
It is identical as the process of embodiment 9, it the difference is that only, the mass percent of Arabic gum is 0.5%；
Embodiment 32
It is identical as the process of embodiment 9, it the difference is that only, the mass percent of Arabic gum is 0.7%；
Embodiment 33
It is identical as the process of embodiment 9, it the difference is that only, the mass percent of Arabic gum is 1%；
Embodiment 34
It is identical as the process of embodiment 9, it the difference is that only, the Arabic gum that mass percent is 0.3% is replaced
The xanthan gum for being 0.1% for mass percent；
Embodiment 35
It is identical as the process of embodiment 9, it the difference is that only, the Arabic gum that mass percent is 0.3% is replaced
The xanthan gum for being 0.5% for mass percent；
Embodiment 36
It is identical as the process of embodiment 19, it the difference is that only, the Arabic gum that mass percent is 0.3% is replaced
The xanthan gum for being 1% for mass percent；
Embodiment 37
It is identical as the process of embodiment 9, it the difference is that only, the Arabic gum that mass percent is 0.3% is replaced
The xanthan gum for being 2% for mass percent；
Embodiment 38
It is identical as the process of embodiment 9, it the difference is that only, the Arabic gum that mass percent is 0.3% is replaced
The xanthan gum for being 3% for mass percent；
Embodiment 39
It is identical as the process of embodiment 9, it the difference is that only, the Arabic gum that mass percent is 0.3% is replaced
The xanthan gum for being 4% for mass percent.
The purpose of embodiment 9 and embodiment 30- embodiment 39 is that screening to stabilizer and stabilizer mass percent are excellent
Change research, to choose the mass percent that stabilizer under effect optimal situation is killed in suppression.In order to screen suitable stabilizers and optimization
The best in quality percentage of stabilizer, solution B Central Asia sodium nitrate concentration are set as 0.206%, glacial acetic acid mass percent in solution A
It is set as 0.72%, dodecyl benzyl dimethyl ammonium chloride mass percent is set as 0.48%, and not homogeneity is arranged in two kinds of stabilizers
Measure percentage gradient, wherein the Arabic gum setting 0.1% in embodiment 9 and embodiment 30- embodiment 33,0.3%, 0.5%,
0.7% and 1% totally 5 gradients, xanthan gum mass percent setting 0.1% in embodiment 34- embodiment 39,0.5%, 1%,
2%, 3% and 4% totally 6 gradients, after the solution A of the concentration containing different stabilizers mixes constant volume 100mL with solution B, 1000 revs/min
Revolving speed stirs 3min, and measurement foam is held time.The results show that two kinds of stabilizers are easily soluble in water；With Arabic gum and ten
When dialkyl dimethyl benzyl ammonium chloride compatibility, liquid milky, no precipitating；Xanthan gum and dodecyl dimethyl Benzylmagnesium chloride
When ammonium compatibility, a small amount of precipitating can be generated.When Arabic gum mass percent is 0.5%, the foam of generation is held time longest, is put down
Equal foam duration 84min；It when xanthan gum mass percent is 2%, generates foam and holds time longest, average foam maintains
Time 62min.
Embodiment 40
The present embodiment provides the foamaseptics that coccidian oocyst is killed in a kind of suppression, are made of the raw material of following mass percent:
Sodium nitrite 0.136%, glacial acetic acid 0.36%, dodecyl benzyl dimethyl ammonium chloride 0.48%, Arabic gum 0.4%, it is remaining
Amount is water；
The present embodiment also provides the preparation method that the foamaseptic of coccidian oocyst is killed in above-mentioned suppression, and steps are as follows:
(1) according to the mass percent in the formula of the present embodiment, glacial acetic acid, Arabic gum and dimethyl are measured
Base benzyl ammonium chloride, sodium nitrite and water, it is spare；
(2) the weighed glacial acetic acid of step (1), Arabic gum and dodecyl benzyl dimethyl ammonium chloride are uniformly mixed,
Obtain solution A；Sodium nitrite and water are mixed to get solution B；
(3) solution B of the solution A of step (2) and step (2) is stirred into 3min, shape under the mixing speed of 1000r/min
At foam disinfectant liquid.
Embodiment 41
It is identical as the process of embodiment 40, it the difference is that only, the glacial acetic acid that mass percent is 0.36% is replaced
The sodium nitrite that mass percent is 0.136% is replaced with 0.206% Asia by the glacial acetic acid for being 0.72% for mass percent
Sodium nitrate；
Embodiment 42
It is identical as the process of embodiment 40, it the difference is that only, the glacial acetic acid that mass percent is 0.36% is replaced
The sodium nitrite that mass percent is 0.136% is replaced with 0.34% Asia by the glacial acetic acid for being 1.44% for mass percent
Sodium nitrate；
Comparative example 12
Positive control is the cyclophosphamide of 3 μ g/mL in S9 metabolism activation system；
Comparative example 13
Positive control is the methyl methylsulfonate of 10 μ g/mL in disactivation service system.
The sample of embodiment 40- embodiment 42 and comparative example 1 and comparative example 12- comparative example 13 has been carried out coccidia ovum by us
Capsule disinfectant is to the gene mutation Effect study of in vitro culture mammalian cell, that is, by embodiment 40- embodiment 42 and comparison
The sample of example 1 and comparative example 12- comparative example 13 acts on 2h with mouse lymphoma cell L5178Y respectively.
Mutagenesis of the 5 coccidian oocyst disinfectant of table to L5178Y cell
Note: RSG: relative suspension growth；MF: the frequency of mutation.
Embodiment 40- embodiment 42 is the toxicity detection research to thimerosal, with GB15193.20-2014 " external lactation
Class cell TK gene mutation test " it is foundation, selection mouse lymphoma cell L5178Y is experimental subjects, detects ball of the invention
Worm's ovum capsule disinfectant acts on the gene mutation of in vitro culture mammalian cell.Experiment activation and under the conditions of two kinds of disactivation into
It goes, positive control is the cyclophosphamide of 3 μ g/mL in S9 metabolism activation system；Positive control is 10 μ g/ in disactivation service system
The methyl methylsulfonate of mL.The results are shown in Table 5, under activation and non-activated condition, coccidian oocyst disinfectant pair of the invention
L5178Y cell TK gene is without mutagenesis.
Embodiment 43
The present embodiment provides the foamaseptics that coccidian oocyst is killed in a kind of suppression, are made of the raw material of following mass percent:
Sodium nitrite 3.6%, glacial acetic acid 5%, dodecyl benzyl dimethyl ammonium chloride 2.4%, Arabic gum 0.4%, surplus are water；
The present embodiment also provides the preparation method that the foamaseptic of coccidian oocyst is killed in above-mentioned suppression, and steps are as follows:
(1) according to the mass percent in the formula of the present embodiment, glacial acetic acid, Arabic gum and dimethyl are measured
Base benzyl ammonium chloride, sodium nitrite and water, it is spare；
(2) the weighed glacial acetic acid of step (1), Arabic gum and dodecyl benzyl dimethyl ammonium chloride are uniformly mixed,
Obtain solution A；Sodium nitrite and water are mixed to get solution B；
(3) solution B of the solution A of step (2) and step (2) is stirred into 5min, shape under the mixing speed of 1500r/min
At foam disinfectant liquid.
The sample of embodiment 43 and comparative example 1 has been carried out 5 times of concentration coccidian oocyst thimerosals to metal protection by us
The research of effect, that is, embodiment 43 and the sample of comparative example 1 are acted on carbon steel, stainless steel, aluminium and copper respectively respectively
72h。
65 times of concentration coccidian oocyst thimerosals of table act on metal protection
The purpose of embodiment 43 and comparative example 1 is to detect to study to metal protection for thimerosal, prepares 5% glacial acetic acid
(non-Sporulated ovum is killed in 5 times of suppressions to+0.4% Arabic gum of+2.4% dodecyl benzyl dimethyl ammonium chloride of+3.6% sodium nitrite
The concentration of capsule), as a control group by using deionized water (comparative example 1), carry out metal protection test.Select carbon steel, no
Becoming rusty, totally 4 kinds of common metal disks are soaked in 72h in thimerosal and water respectively for steel, aluminium and copper, then calculate before impregnating and after impregnating
Weight change, that is, rate of metal corrosion (R) of metal disk judges the metal protection of thimerosal according to " disinfection technology standard ".
The results are shown in Table 6, and the coccidian oocyst thimerosal of 5 times of concentration is non-corrosive to stainless steel, aluminium and copper-based, has slightly to carbon steel
Corrosiveness, when compared with the deionized water with control group, the two is without significant difference, therefore the corrosivity of disinfectant of the invention
It is low.
In conclusion containing mass percent are as follows: sodium nitrite 0.103%~0.41%, glacial acetic acid 0.18%~1.44%,
Thimerosal acts on 2h's or more to coccidia unsporulated oocysts action time 60min~240min, thimerosal and Sporulated Oocysts
In the case of play best inhibitory or killing effect, in addition the study found that foam hold time it is longest be mass percent be 0.48%
Dodecyl benzyl dimethyl ammonium chloride, foam average duration 42min, the Arabic gum that mass percent is 0.5% produce
Raw foam is held time longest, average duration 84min, at the same activate and non-activated condition under, coccidia ovum of the invention
Capsule disinfectant is to L5178Y cell TK gene without mutagenesis.In addition, the result of study by example 1~43 illustrates, this hair
Bright coccidian oocyst disinfectant can efficiently press down the activity of kill worm unsporulated oocysts and Sporulated Oocysts, and no cause cytogene is prominent
It is changed into use, is a kind of efficient, safe and environmentally friendly coccidian oocyst environment disinfectant to animal house cage without obvious corrosivity.
Those skilled in the art various changes and modifications can be made to the invention without departing from spirit of the invention and
Range, if these modifications and changes of the present invention is within the scope of the claims of the present invention and its equivalent technology, then originally
Invention is also intended to include these modifications and variations.
Claims (8)
1. a kind of press down the foamaseptic for killing coccidian oocyst, which is characterized in that be made of the raw material of following mass percent: nitrous
Sour sodium 0.103%~0.41%, glacial acetic acid 0.18%~1.44%, foaming agent 0.1%~4%, stabilizer 0.1%~4%, it is remaining
Amount is water.
2. the foamaseptic that coccidian oocyst is killed in a kind of suppression according to claim 1, which is characterized in that the foaming agent
Mass percent is 0.32%~0.64%, and the foaming agent is dodecyl benzyl dimethyl ammonium chloride.
3. the foamaseptic that coccidian oocyst is killed in a kind of suppression according to claim 1, which is characterized in that the foaming agent
Mass percent is 0.1%~3%, and the foaming agent is hydroxyethyl cellulose.
4. the foamaseptic that coccidian oocyst is killed in a kind of suppression according to claim 1, which is characterized in that the foaming agent
Mass percent is 0.5%~4%, and the foaming agent is neopelex.
5. the foamaseptic that coccidian oocyst is killed in a kind of suppression according to claim 1, which is characterized in that the stabilizer
Mass percent is 0.1%~1%, and the stabilizer is Arabic gum.
6. it is according to claim 1 it is a kind of press down kill the foamaseptic of coccidian oocyst, which is characterized in that the stabilizer is
Xanthan gum.
7. the preparation method that the foamaseptic of coccidian oocyst is killed in a kind of suppression according to claim 1, which is characterized in that packet
Include following steps:
(1) each raw material: 0.103%~0.41% sodium nitrite, 0.18%~1.44% is weighed according to following mass percent
Glacial acetic acid, 0.1%~4% foaming agent, 0.1%~4% stabilizer, surplus is water, spare；
(2) the weighed glacial acetic acid of step (1), foaming agent and stabilizer are uniformly mixed, obtain solution A；Step (1) is weighed
Sodium nitrite is uniformly mixed with water, obtains solution B；
(3) solution A and the solution B mixing obtained step (2), stirs evenly, and obtains the foamaseptic that coccidian oocyst is killed in suppression.
8. the preparation method that the foamaseptic of coccidian oocyst is killed in a kind of suppression according to claim 7, which is characterized in that step
Suddenly the revolving speed of (3) described stirring is 1000-1500r/min, mixing time 3-5min.
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