CN110057801A - A kind of ratio fluorescent probe and its hydrogen peroxide and glucose detection application based on aggregation-induced emission property - Google Patents
A kind of ratio fluorescent probe and its hydrogen peroxide and glucose detection application based on aggregation-induced emission property Download PDFInfo
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- CN110057801A CN110057801A CN201910387097.9A CN201910387097A CN110057801A CN 110057801 A CN110057801 A CN 110057801A CN 201910387097 A CN201910387097 A CN 201910387097A CN 110057801 A CN110057801 A CN 110057801A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
- G01N2021/6432—Quenching
Abstract
The present invention relates to a kind of ratio fluorescent probe and its hydrogen peroxide based on aggregation-induced emission property and glucose detection applications, belong to the crossing domain of chemistry and materialogy.The probe is made of tetraphenylethylene sulfonate and the coated gold nano cluster of bovine serum albumin(BSA).The electrostatic adsorption of the two makes tetraphenylethylene sulfonate generate aggregation-induced emission phenomenon, and mixed solution is respectively between 410-550nm and 550-850nm in double launch wavelengths, and the ratio of the two is different, and the color for mixing the presentation of fluorescence probe is different.The aggregation-induced emission of tetraphenylethylene sulfonate can be used as reference fluorescent color, according to double emission peak ratio fluorescent changing value (I0‑I)/I0Linear changing relation between analyte concentration C realizes the detection of hydrogen peroxide and glucose, which has the advantages that simple, quick and Visual retrieval hydrogen peroxide and glucose.
Description
Technical field
The present invention relates to a kind of ratio fluorescent probes and its hydrogen peroxide and glucose based on aggregation-induced emission property
Detection application is related to nano material preparation and hydrogen peroxide and glucose detection, belongs to the crossing domain of chemistry and materialogy.
Background technique
Hydrogen peroxide (H2O2) it is a kind of important active oxygen, it is widely used in industry and home washings, bleaching and doctor
With disinfection, but the residual peroxide of Coal Gas Washing Cycling Water can carry out serious, irreversible oxidative damage to aquatic biozone.
On the other hand, hydrogen peroxide plays the part of important role as a kind of important biogenic signaling molecule in a variety of bioprocess.Cause
This, hydrogen peroxide detection is crucial in environmental protection and field of biomedical research.
Glucose is the important substance in human body metabolic balance, is the energy source for maintaining human life activity.Blood glucose water
It is flat be human body whether one of Jian Kang index, excessively high or too low blood glucose level implies diabetes or hypoglycemia, because
This, the detection of blood sugar concentration is particularly significant in clinical diagnosis.
The method detected currently used for hydrogen peroxide and glucose content has very much, including electrochemical process, fluorescence detection side
Method, near infrared spectroscopy etc., wherein fluorescence probe detection have many advantages, such as simple, at low cost, high sensitivity.For example, document
“H2O2-sensitive quantum dots for the label-free detection of glucose,Talanta,
2010,82 (3): 997-1002. " report, hydrogen peroxide is quenched cadmium telluride-cadmiumsulfide quantum dot and shines, and grape is glycoxidative
Enzymatic glucose generates gluconic acid and hydrogen peroxide, and the simple and quick inspection of glucose may be implemented by detecting hydrogen peroxide
It surveys.For another example document " Fluorescent detection of hydrogen peroxide and glucose with
polyethyleneimine-templated Cu nanoclusters,Spectrochimica Acta Part A:
Molecular and Biomolecular Spectroscopy, 2014,118:315-320. " report, with polyethyleneimine
Coated copper nano-cluster is fluorescence probe, and the fluorescence of hydrogen peroxide quenching fluorescence probe, glucose is in glucose oxidase catalysis
Effect is lower to generate gluconic acid and hydrogen peroxide, to realize the simple and quick detection of hydrogen peroxide and glucose.In recent years, than
Color fluorescent technique is due to its highly sensitive and accuracy, and to the inherent correct influences of environment, causes the extensive concern of people.
Colorimetric fluorescent technique is that detection is realized in the fluorescence ratio variation based on detection both front and back different wave length, glimmering compared to altered
The ratio of light probe, color change can be with the naked eye clearly discernable, to realize half-quantitative detection.For example, document
“Graphitic carbon nitride nanosheets-based ratiometric fluorescent probe for
highly sensitive detection of H2O2and glucose,ACS Applied Materials&
Interfaces, 2016,8 (49): 33439-33445. " reports a kind of high sensitivity based on azotized carbon nano piece and detected
The ratio fluorescent probe of hydrogen oxide and glucose, o-phenylenediamine is in the presence of horseradish peroxidase by hydrogen peroxide oxidation, oxygen
Change Product bulk and its fluorescence is quenched in azotized carbon nano piece, and generate a new emission peak, is catalyzed and produces in glucose oxidase
On the basis of raw hydrogen peroxide, high throughput, the low cost detection of glucose may be implemented.For another example document " A dual-
emission nanohybrid of gold nanoclusters and carbon dots for ratiometric
fluorescence detection of reactive oxygen species and glucose,Journal of
Biomedical Nanotechnology, 2017,13 (11): 1425-1434. " reports one kind by carbon dots (CDs) and Jenner
What rice cluster (Au NCs) was self-assembled into is used to detect hydrogen peroxide and glucose ratio fluorescence probe.Further for example, document " A
novel dual response ratiometric fluorescent probe for the determination of
H2O2And glucose via etching of silver nanoparticles, Analyst, 2019,144 (4): 1153-
1158. ", which report a kind of derivative carbon dots-nano SiO 2 particle-cadmium telluride quantum dot double-bang firecracker of glycosyl, answers colorimetric fluorescence probe,
When Nano silver grain (Ag NPs) is mixed with probe, the derivative carbon dots of glycosyl are quenched, and after hydrogen peroxide is added, carbon dots fluorescence is extensive
It is multiple, hydrogen peroxide is generated under glucose oxidase effect based on glucose, glucose detection may be implemented.
What above-mentioned fluorescence probe or ratio fluorescent probe were used is all traditional fluorescent material, such as inorganic-quantum-dot, gold
Belong to nano-cluster, carbon dots etc., traditional fluorescent material can only shine in weak solution, can occur under high concentration or state of aggregation certainly
Be quenched, therefore sensitivity, interference detection results can be reduced etc..Aggregation-induced emission (Aggregation-Induced
Emission, AIE) it is that molecule hardly shines in the solution, and luminescence enhancement shows under the coherent condition or solid-state
As.Aggregation-induced emission probe has preferable photostability and portability, and signal with higher/noise ratio is passed in chemistry
There is huge application prospect in the fields such as sense, biological monitoring.
Summary of the invention
The purpose of the present invention is for self-quenching easily occurs in quickly detecting for conventional fluorescent probe, testing result is vulnerable to dry
The problems such as disturbing provides a kind of ratio fluorescent probe and its hydrogen peroxide and glucose detection based on aggregation-induced emission property and answers
With.
To achieve the above object, the present invention adopts the following technical scheme:
A kind of ratio fluorescent probe based on aggregation-induced emission property, by tetraphenylethylene sulfonate and bovine serum albumin
White coated gold nano cluster composition, wherein tetraphenylethylene sulfonate is as reference fluorescent probe, bovine serum albumin(BSA) coating
Gold nano cluster as fluorescence probe, the two mixing building ratio fluorescent probe is different according to the two mixed proportion, mixes molten
Red, yellow or blue is presented in the color of liquid.
A kind of preparation method of the ratio fluorescent probe based on aggregation-induced emission property, the specific steps are as follows:
Step 1: preparing tetraphenylethylene sulfonate solution, a certain amount of tetraphenylethylene sulfonate is weighed, is dissolved
Yu Shuizhong.
Step 2: preparing the coated gold nano cluster of bovine serum albumin(BSA), 5mL gold chloride (5-20mM) is added to 5mL
In bovine serum albumin solution (20-50mM), adjust pH be alkalinity, 25-60 DEG C reaction 10-24 hours, obtain bovine serum albumin
White coated gold nano cluster (BSA-Au NCs).
Step 3: tetraphenylethylene sulfonate prepared by step 1 is mixed with gold nano cluster prepared by step 2, obtain
To probe；
Complex method described in step 3 are as follows: (chemical structural formula is such as the tetraphenylethylene sulfonate for preparing above-mentioned steps one
Shown in formula 1) the coated gold nano cluster mixing of the bovine serum albumin(BSA) that is synthesized with step 2 of solution (unstressed configuration), bovine serum albumin
White coated gold nano cluster is positively charged, and tetraphenylethylene sulfonate is negatively charged, tetraphenylethylene sulfonate and cow's serum
Electrostatic adsorption occurs for the coated gold nano cluster of albumin, aggregation-induced emission occurs, fluorescence emission wavelengths are in 400-
550nm.Mixed solution fluorescence emission wavelengths are respectively in 400-550nm and 550-850nm to get the Ratio-type emitted to dual wavelength
Fluorescence probe.
Wherein: R1、R2=Cl, Br, I, CnH2n+1, n=0,1,2,3 ... n.
Preferably, tetraphenylethylene sulfonate described in step 1, works as n=0, i.e. R1、R2=H, i.e. tetraphenylethylene sulphur
Hydrochlorate is bis- [4- (3- sulfonic acid propoxyl group) phenyl] -1, the 2- talan sodium salts (BSPOTPE) of 1,2-, configuration concentration 0.1mg/
mL；The coated gold nano cluster concentration of the bovine serum albumin(BSA) prepared in step 2 is 5mM (with Au in gold chloride3+Ion it is dense
Degree meter)；The volume ratio of tetraphenylethylene sulfonate solution and the coated gold nano cluster of bovine serum albumin(BSA) is 1 in step 3:
0.5。
The invention further relates to the methods detected using the probe to hydrogen peroxide or glucose:
Solution to be measured is added drop-wise in the probe solution, is reacted after a certain period of time, if range estimation has to probe solution color
Variation then illustrates to contain hydrogen peroxide or glucose in solution to be measured；
Record fluorescence intensity ratio (the i.e. F at initial probe 680nm and 490nm680/F490)I0, by known various concentration C
Solution to be measured be added separately in probe solution, record probe solution color change stablize after fluorescence intensity ratio I, according to
Fluorescence intensity ratio changing value (I0-I)/I0Changing rule between solution concentration C to be measured, obtains linear relationship equation: (I0-
I)/I0=aC+b, a are linear equation slope, and b is linear equation intercept；
(1) ratio fluorescent probe in detecting
The quantitative detection of target analytes: launch wavelength can be quenched in the bovine serum albumin of 550-850nm in target analytes
The fluorescence of white coated gold nano cluster, and fluorescence of the tetraphenylethylene sulfonate in 400-550nm is unaffected, so the latter
It can be used as fluorescence background reference color and construct double emission ratios type fluorescence probes.Record fluorescence intensity ratio I and I0, I and I0Respectively
Ratio (the F of fluorescence intensity when for sample concentration and blank at launch wavelength 680nm and the fluorescence intensity at 490nm680/
F490), calculate ratio fluorescent changing value (I0-I)/I0With the linear relationship between target analyte concentration C, with linear relationship equation
Carry out the quantitative analysis of unknown concentration sample.
The visualization half-quantitative detection of target analytes: under the irradiation of 365nm ultraviolet light, the analyte shadow of various concentration
The ratio for ringing the fluorescence intensity at launch wavelength 680nm and the fluorescence intensity at 490nm, causes according to the change of analyte concentration
Color-yellow color-green variation is presented in solution colour.The responsiveness of analyte can be with the naked eye told according to color change,
The half-quantitative detection for realizing analyte, it is convenient, accurate in whole process.
Ratiometric fluorescent probe of the invention detects hydrogen peroxide: hydrogenperoxide steam generator mixed with ratio fluorescent probe,
It is added buffer (pH 5-8), after constant volume, 30min or more is reacted at 25-50 DEG C, then with luminoscope scanning 410-850nm's
Fluorescence emission wavelengths record fluorescence intensity ratio I and I0, I and I0Respectively sample concentration and when blank at launch wavelength 680nm
Fluorescence intensity and 490nm at fluorescence intensity ratio (F680/F490), according to double emission peak ratio fluorescent changing value (I0-
I)/I0Relationship between hydrogenperoxide steam generator concentration C establishes standard curve, and mistake in sample to be tested can be obtained in contrast standard curve
The concentration of hydrogen oxide.Under 365nm ultraviolet light, visually colorimetric method carries out semi-quantitative analysis
Ratiometric fluorescent probe of the invention detects glucose: glucose solution mixed with glucose oxidase solution,
It is added buffer (pH 5-8), ratio fluorescent probe is added, adds water constant volume, after mixing, 30min or more is reacted at 25-50 DEG C, so
Afterwards with the fluorescence emission wavelengths of luminoscope scanning 410-850nm, fluorescence intensity ratio I and I is recorded0, I and I0Respectively sample is dense
Ratio (the F of fluorescence intensity when degree and blank at launch wavelength 680nm and the fluorescence intensity at 490nm680/F490), according to double
Emission peak ratio fluorescent changing value (I0-I)/I0Relationship between glucose concentration C establishes standard curve, contrast standard
The concentration of glucose in sample to be tested can be obtained in curve.Under 365nm ultraviolet light, visually colorimetric method carries out sxemiquantitative point
Analysis.
The beneficial effects of the present invention are:
1, the electrostatic adsorption based on bovine serum albumin(BSA) coated gold nanoclusters and tetraphenylethylene sulfonate, causes
Tetraphenylethylene sulfonate aggregation-induced emission characteristic and the coated gold nanoclusters of bovine serum albumin(BSA) issue red fluorescence
Property, construct ratio fluorescent probe, the ratio of the two is different, and red-yellow-green color change is presented in mixed solution.
2, the hydrogen peroxide that hydrogen peroxide or the reaction of glucose and glucose oxidase generate will lead to bovine serum albumin
The red fluorescence of white coated gold nanoclusters is quenched, the blue-green fluorescent without influencing tetraphenylethylene sulfonate, therefore four benzene
The aggregation-induced emission of base vinyl sulfonate can be used as reference fluorescent color, according to double emission peak ratio fluorescent changing value (I0-
I)/I0Linear changing relation between analyte concentration C realizes the detection of target analytes.Reference fluorescent color can reduce bottom
Data error caused by the factors such as object, external environment and instrument condition variation etc., can be used for the detection of actual sample.The ratio is visited
Needle set have it is quick, simple, be able to use luminoscope and realize quantitative detection and realize visualization half-quantitative detection under ultraviolet light
Advantage is for the first time by tetraphenylethylene sulfonate aggregation-induced emission probe caused by the coated gold nanoclusters of bovine serum albumin(BSA)
As mixing ratio fluorescent probe, for Ratio-type and Visual retrieval hydrogen peroxide and glucose, in hydrogen peroxide and grape
Sugared field of fast detection has boundless application prospect, and is expected to be used for the detection of other small molecules.
Detailed description of the invention
The schematic illustration of Fig. 1 Ratiometric fluorescent probe detection hydrogen peroxide and glucose；
Bis- [4- (3- sulfonic acid propoxyl group) the phenyl] -1,2- talan sodium salts (BSPOTPE) of the 1,2- of Fig. 2 difference ratio and
The coated gold nano cluster of bovine serum albumin(BSA) (BSA-AuNCs) fluorescence emission spectrogram of compound；
Fig. 3 various concentration hydrogen peroxide and ratio fluorescent changing value (I0-I)/I0Standard curve；
Fig. 4 different glucose and ratio fluorescent changing value (I0-I)/I0Standard curve；
Selective enumeration method of Fig. 5 method to glucose and other carbohydrates.
Specific embodiment
The present invention will be further described with embodiment with reference to the accompanying drawing.
Embodiment 1
A kind of ratio fluorescent probe based on aggregation-induced emission property is tetraphenylethylene sulfonate and bovine serum albumin
The composite material of white coated gold nano cluster, different according to the two mixed proportion, red, yellow is presented in the color of composite material
Or blue；
A kind of preparation method of the ratio fluorescent probe based on aggregation-induced emission property, the specific steps are as follows:
Step 1: preparing tetraphenylethylene sulfonate solution, bis- [4- (the 3- sulfonic acid propoxyl group) benzene of a certain amount of 1,2- are weighed
Base] -1,2- talan sodium salt (BSPOTPE), it is dissolved in the water, concentration 0.1mg/L.
Step 2: preparing the coated gold nano cluster of bovine serum albumin(BSA), 5mL gold chloride (10mM) is added to 5mL ox
In serum albumin solution (50mM), adjusting pH with sodium hydroxide is 10,37 DEG C of reactions 12 hours, obtains bovine serum albumin(BSA) packet
The gold nano cluster (BSA-Au NCs) of quilt, concentration are 5mM (with Au in gold chloride3+The densimeter of ion)；
Step 3: by the ox blood of the tetraphenylethylene sulfonate solution (0.1mg/L) of step 1 preparation and step 2 preparation
The pure coated gold nano cluster of albumen (5mM) obtains ratio fluorescent probe, as shown in Fig. 2, four with the mixing of arbitrary volume ratio
The ratio of phenylethylene sulfonate and the coated gold nano cluster of bovine serum albumin(BSA) is different, obtains the launch wavelength of fluorescence probe
Ratio (the F of the fluorescence intensity at fluorescence intensity and 490nm at 680nm680/F490) different, it presents under ultraviolet light different
Color, when volume ratio BSPOTPE:BSA-Au NCs=1:1, fluorescence probe takes on a red color, volume ratio BSPOTPE:BSA-Au NCs
When=1:0.5, fluorescence probe is in orange, and when volume ratio BSPOTPE:BSA-Au NCs=1:0.1, fluorescence probe is in green.
Embodiment 2
The detection of hydrogen peroxide: the coated gold nano cluster of 12.5 μ L bovine serum albumin(BSA) (5mM, with Au in gold chloride is taken3+
The densimeter of ion) and bis- [4- (3- sulfonic acid propoxyl group) the phenyl] -1,2- talan sodium salts (0.1mg/L) of 25 μ L 1,2- it is mixed
Synthesize ratio fluorescent probe.By 50 μ L H2O2Solution (1,2,3,4,5,6,7,8mM) is mixed with above-mentioned ratio fluorescent probe, is added
100 μ L PBS buffer solution (10mM, pH7) are settled to 500 μ L, react 30min at 37 DEG C, then scan 410- with luminoscope
The fluorescence emission wavelengths of 850nm record fluorescence intensity ratio I and I0, I and I0Respectively sample concentration and launch wavelength when blank
Ratio (the F of the fluorescence intensity at fluorescence intensity and 490nm at 680nm680/F490), obtain ratio fluorescent changing value (I0-I)/
I0Linear relationship (I between concentration of hydrogen peroxide C0-I)/I0=0.0322C+0.5347, as shown in Figure 3.With the linear pass
System can carry out quantitative analysis to hydrogen peroxide in unknown sample.Under 365nm ultraviolet light, visually colorimetric method carries out half
Quantitative analysis.
Embodiment 3
Glucose detection: the coated gold nano cluster of 12.5 μ L bovine serum albumin(BSA) (5mM, with Au in gold chloride is taken3+Ion
Densimeter) and bis- [4- (3- sulfonic acid propoxyl group) the phenyl] -1,2- talan sodium salts (0.1mg/L) of 25 μ L 1,2- be mixed into
Ratio fluorescent probe.By 100 μ L glucose solution (1,2,3,4,5,6,7,8,9mM) and 100 μ L glucose oxidizing ferment (0.5mg/
L) solution mixes, and 100 μ L PBS buffer solution (10mM, pH 7) are added, is added in ratio fluorescent probe, water is added to be settled to 500 μ
L after mixing, reacts 30min at 37 DEG C, then with the fluorescence emission wavelengths of luminoscope scanning 410-850nm, record fluorescence intensity
Ratio I and I0, I and I0Respectively sample concentration and fluorescence intensity when blank at launch wavelength 680nm and the fluorescence at 490nm
Ratio (the F of intensity680/F490), obtain ratio fluorescent changing value (I0-I)/I0Linear relationship (I between concentration of glucose C0-
I)/I0=0.0705C+0.0407, as shown in Figure 4.With the linear relationship, glucose in unknown sample can quantitatively be divided
Analysis.Under 365nm ultraviolet light, visually colorimetric method carries out semi-quantitative analysis.
Embodiment 4
Glucose in serum detection: the 12.5 coated gold nano cluster of μ L bovine serum albumin(BSA) (5mM, in gold chloride are taken
Au3+The densimeter of ion) and bis- [4- (3- sulfonic acid propoxyl group) the phenyl] -1,2- talan sodium salts (0.1mg/L) of 25 μ L 1,2-
It is mixed into ratio fluorescent probe.100 μ L serum are mixed with 100 μ L glucose oxidizing ferment (0.5mg/L) solution, 100 μ L are added
PBS buffer solution (10mM, pH 7) after mixing, is added in ratio fluorescent probe, and water is added to be settled to 500 μ L, after mixing, 37 DEG C
Lower reaction 30min or more, then with luminoscope scanning 410-850nm fluorescence emission wavelengths, record fluorescence intensity ratio I and
I0, I and I0The respectively ratio of sample concentration and fluorescence intensity when blank at launch wavelength 680nm and the fluorescence intensity at 490nm
It is worth (F680/F490), according to ratio fluorescent changing value (I0-I)/I0Linear relationship (I between concentration of glucose C0-I)/I0=
0.0705C+0.0407 calculates blood-sugar content, and compares with hospital's traditional technique in measuring value, as a result as shown in the table, with tradition
Idea is compared, and the accuracy of the method is higher.
Embodiment 5
Glucose selective detection: the 12.5 coated gold nano cluster of μ L bovine serum albumin(BSA) (5mM, in gold chloride are taken
Au3+The densimeter of ion) and bis- [4- (3- sulfonic acid propoxyl group) the phenyl] -1,2- talan sodium salts (0.1mg/L) of 25 μ L 1,2-
It is mixed into ratio fluorescent probe.By 100 μ L glucoses, sucrose, fructose, maltose, xylose solution (concentration is 5mM) and 100 μ
The mixing of L glucose oxidizing ferment (0.5mg/L) solution, is added 100 μ L PBS buffer solution (10mM, pH 7), is added to ratio fluorescent
In probe, water is added to be settled to 500 μ L, after mixing, 30min is reacted at 37 DEG C, then with the fluorescence of luminoscope scanning 410-850nm
Launch wavelength records fluorescence intensity ratio I and I0, I and I0Respectively sample concentration and when blank it is glimmering at launch wavelength 680nm
Ratio (the F of fluorescence intensity at luminous intensity and 490nm680/F490), as shown in figure 5, only in the presence of glucose, it is glimmering
Intensity ratio changes greatly, and sucrose, fructose, maltose, xylose are smaller to the interference of detection.Grape may be implemented in the method
The specific detection of sugar.
In conclusion the above is merely preferred embodiments of the present invention, being not intended to limit the scope of the present invention.
All within the spirits and principles of the present invention, any modification, equivalent replacement, improvement and so on should be included in of the invention
Within protection scope.
Claims (7)
1. a kind of ratio fluorescent probe based on aggregation-induced emission property is used for hydrogen peroxide and glucose detection, feature exists
In: it is made of tetraphenylethylene sulfonate and the coated gold nano cluster of bovine serum albumin(BSA)；Wherein tetraphenylethylene sulfonate
As reference fluorescent probe, the coated gold nano cluster of bovine serum albumin(BSA) is as fluorescence probe, the two mixing building fluorescence ratio
Rate probe, different according to the two mixed proportion, red, yellow or blue is presented in the color of mixed solution；Hydrogen peroxide or Portugal
The red that the hydrogen peroxide that grape sugar and glucose oxidase reaction generate will lead to the coated gold nanoclusters of bovine serum albumin(BSA) is glimmering
Optical quenching, the blue-green fluorescent without influencing tetraphenylethylene sulfonate, according to double emission peak ratio fluorescent changing values and analysis
Variation relation between object concentration realizes the detection of hydrogen peroxide and glucose.
2. a kind of preparation method of the ratio fluorescent probe based on aggregation-induced emission property, it is characterised in that: specific steps are such as
Under:
Step 1: preparing tetraphenylethylene sulfonate solution；
Step 2: preparing the coated gold nano cluster of bovine serum albumin(BSA)；
Step 3: tetraphenylethylene sulfonate prepared by step 1 is mixed with gold nano cluster prepared by step 2, visited
Needle；The coated gold nano cluster of bovine serum albumin(BSA) is positively charged, and tetraphenylethylene sulfonate is negatively charged, tetraphenylethylene sulphur
Electrostatic adsorption occurs for hydrochlorate and the coated gold nano cluster of bovine serum albumin(BSA), and aggregation-induced emission, fluorescence hair occurs
Ejected wave is grown in 400-550nm；Mixed solution fluorescence emission wavelengths respectively 400-550nm and 550-850nm to get arrive dual wavelength
The Ratiometric fluorescent probe of transmitting.
3. a kind of preparation method of the ratio fluorescent probe based on aggregation-induced emission property as claimed in claim 2, special
Sign is: tetraphenylethylene sulfonate described in step 1 is at least at least to contain containing a tetraphenyl ethylene molecular structure skeleton
There are two sulfonic acid with group, and structural formula is such as shown in (1).
Wherein: R1、R2=Cl, Br, I, CnH2n+1, n=0,1,2,3 ... n.
4. a kind of preparation method of the ratio fluorescent probe based on aggregation-induced emission property as claimed in claim 2, special
Sign is: the method for the coated gold nano cluster of bovine serum albumin(BSA) is prepared described in step 2 are as follows: by 5mL gold chloride (5-
20mM) be added in 5mL bovine serum albumin solution (20-50mM), adjust pH be alkalinity, 25-60 DEG C reaction 10-24 hours,
Obtain the coated gold nano cluster of bovine serum albumin(BSA) (BSA-Au NCs).
5. a kind of preparation method of the ratio fluorescent probe based on aggregation-induced emission property as claimed in claim 2, special
Sign is: preferred, tetraphenylethylene sulfonate described in step 1 is bis- [4- (3- sulfonic acid propoxyl group) phenyl] -1, the 2- bis- of 1,2-
Styrene sodium salt (BSPOTPE), configuration concentration 0.1mg/mL；The coated gold nano cluster of bovine serum albumin(BSA) described in step 2
Concentration is 5mM (with Au in gold chloride3+The densimeter of ion)；Tetraphenylethylene sulfonate solution and bovine serum albumin in step 3
The volume ratio of white coated gold nano cluster is 1:0.5.
6. the semi-quantitative method that hydrogen peroxide or glucose are detected using ratio fluorescent probe as described in claim 1,
It is characterized by: the solution to be measured of known various concentration C is added separately to probe solution under the irradiation of 365nm ultraviolet light
In, then solution to be measured is added drop-wise in the probe solution, if mesh by the color after record probe solution color change is stable again
It measures probe solution color to change, then illustrates containing hydrogen peroxide or glucose in solution to be measured, according to color change and
Know that the sample to be tested color of concentration compares, realizes half-quantitative detection.
7. the method that quantitative detection is carried out to hydrogen peroxide or glucose using ratio fluorescent probe as described in claim 1,
It is characterized in that: the fluorescence intensity ratio I at record initial probe 680nm and 490nm0, I0=F680/F490, by known various concentration
The solution to be measured of C is added separately in probe solution, the fluorescence intensity ratio I after record probe solution color change is stable, root
According to fluorescence intensity ratio changing value (I0-I)/I0With the changing rule of solution concentration C to be measured, linear relationship equation: (I is obtained0-
I)/I0=aC+b, a are linear equation slope, and b realizes the sample of unknown concentration according to the linear equation for linear equation intercept
Detection.
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN111504961A (en) * | 2020-03-31 | 2020-08-07 | 南昌大学 | Fluorescent sensor based on glutathione gold nanoclusters and application thereof |
| CN111504961B (en) * | 2020-03-31 | 2023-05-02 | 南昌大学 | Fluorescent phytic acid detection method based on glutathione gold nanoclusters |
| CN112891564A (en) * | 2021-02-03 | 2021-06-04 | 山东大学 | Preparation method of positive charge gold nanoparticle material by cation-induced self-assembly |
| CN113046059A (en) * | 2021-03-22 | 2021-06-29 | 中国科学院大学温州研究院(温州生物材料与工程研究所) | Nano gold cone composite material with photo-thermal/photodynamic therapy performance and preparation method and application thereof |
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| CN116067926A (en) * | 2022-12-19 | 2023-05-05 | 广东省大湾区华南理工大学聚集诱导发光高等研究院 | Occult blood revealing reagent based on aggregation-induced emission molecules, and preparation method and application thereof |
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