CN110054704A - The method that macroreticular resin purification bean jelly grass polysaccharide is combined using ammonium sulfate and CTAB precipitating - Google Patents
The method that macroreticular resin purification bean jelly grass polysaccharide is combined using ammonium sulfate and CTAB precipitating Download PDFInfo
- Publication number
- CN110054704A CN110054704A CN201910278506.1A CN201910278506A CN110054704A CN 110054704 A CN110054704 A CN 110054704A CN 201910278506 A CN201910278506 A CN 201910278506A CN 110054704 A CN110054704 A CN 110054704A
- Authority
- CN
- China
- Prior art keywords
- polysaccharide
- precipitating
- ammonium sulfate
- ctab
- chinese mesona
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/006—Heteroglycans, i.e. polysaccharides having more than one sugar residue in the main chain in either alternating or less regular sequence; Gellans; Succinoglycans; Arabinogalactans; Tragacanth or gum tragacanth or traganth from Astragalus; Gum Karaya from Sterculia urens; Gum Ghatti from Anogeissus latifolia; Derivatives thereof
Abstract
The present invention relates to effective ingredients in plant to refine processing and treating method field, and in particular to a method of combine D301 macroreticular resin to refine bean jelly grass polysaccharide by ammonium sulfate and CTAB precipitating.The Thick many candies that Chinese mesona herb is obtained after pretreatment and extraction combine the method for D301 macroporous resin treatment to achieve the purpose that refinishing polyose with ammonium sulfate and CTAB precipitating.After handling bean jelly grass polysaccharide using the method, the residual of protein and small molecular weight impurity can be substantially reduced, product purity is improved, obtains higher polysaccharide retention rate, percent of decolourization, albumen removal efficiency and yield, while can also preferably retain the bioactivity of polysaccharide.Optimal conditions provided by the present invention are at low cost, easy to operate, effect is good, small to the destruction of bean jelly grass polysaccharide, are suitable for industrialized production.
Description
Technical field
The invention belongs to effective ingredients in plant to refine processing and treating method field, be specifically related to utilize ammonium sulfate and CTAB
The method that precipitating combines D301 macroreticular resin purification bean jelly grass polysaccharide.
Background technique
Chinese mesona herb also known as mesona are a kind of edible and medicinal annual herb plants.In China, it has been used as weight
The medicine and food plant resource wanted, the food composition being typically used as in herb tea and jam type dessert and the production of edible gel.
It has many bioactivity include it is anti-oxidant, protect heatstroke, liver protection anti-hypertension and it is hypoglycemic the effects of.Polysaccharide is Chinese mesona herb
One of main component, some researches show that bean jelly grass polysaccharide is by rhamnose, arabinose, galactolipin, glucose, xylose, sweet dew
Sugar, galacturonic acid composition.Bean jelly grass polysaccharide can be compounded with other edible glues, have have complementary functions, synergistic work
With can develop thickening whereby, be gelled, serve as dietary fiber, stable emulsifying, suspended dispersed etc. and having been permitted a series of multi-functional foods
Product additive, allow to it is more economical, be effectively applied to food processing field and play an important role.Meanwhile Chinese mesona herb
Polysaccharide has multiple biological activities, including antitumor, anti-oxidant, antiviral, hypoglycemic, immunological regulation and other effects.It can be used for making
Make drink and food with health care function, also can be used as the potential replenishers of drug.
In recent years, the extraction of bean jelly grass polysaccharide and the concern by many scientific research personnel is isolated and purified.But from Chinese mesona herb
In in the bean jelly grass polysaccharide that extracts protein content it is higher, and be in dark brown, this not only influences polysaccharide as food additives
Or the appearance of functional food ingredient, and the purifying of subsequent polysaccharide is had a huge impact, so for Chinese mesona herb
The purification of polysaccharide is very necessary.The Deproteinated method of traditional polysaccharide is based on Sevage method, and method is cumbersome, low efficiency,
The organic solvent of use is toxic and influences the bioactivity of polysaccharide；The effect of active carbon Polysaccharide removing pigment is preferable, but the later period
Active carbon powder be not easy to remove, it is therefore desirable to continuing to explore a kind of new refinishing polyose method makes its play maximum exploitation value
Value.
Summary of the invention
The object of the present invention is to provide a kind of methods for refining bean jelly grass polysaccharide, and core of the invention technology is: applying sulphur
Sour ammonium and CTAB precipitating and anion exchange absorbing resin substitute traditional Sevage method, activated carbon method removes bean jelly grass polysaccharide
Albumen, pigment and small molecular weight impurity are suitble to scale industrialization to prepare Chinese mesona herb refined polysaccharide.
To achieve the above object, the technical solution adopted by the present invention is that:
Using ammonium sulfate and CTAB precipitating combine macroreticular resin purification bean jelly grass polysaccharide method, it is characterised in that including with
Lower step: A. pretreatment of raw material；B. hot water extraction prepares Thick many candies；C. ammonium sulfate precipitation；D.CTAB precipitating；E.D301 macropore is inhaled
Attached resin treatment；F. dialysis removal small molecule；G. vacuum freeze drying obtains purification bean jelly grass polysaccharide.
Further, the step is as follows:
A, pretreatment of raw material: impregnating 16-24h with the 60-95% ethyl alcohol of 8-10 times of volume after Chinese mesona herb crushing, and residue is shady and cool
Place air-dries；
B, hot water extraction prepares Thick many candies: the aqueous solution of 10-20 times of volume being added in pretreated Chinese mesona herb, boiling water mentions
1-3h, 120 mesh filtered through gauze of supernatant are taken, filter residue repeats to extract 1-3 times, and merging filtrate is concentrated, and centrifugation obtains Chinese mesona herb
Thick many candies solution；
C, ammonium sulfate precipitation: the ammonium sulfate of Chinese mesona herb Thick many candies solution saturation precipitates 8-12h at 4 DEG C, thick more
The volume ratio of sugar and ammonium sulfate is 1: 1；It is centrifuged off precipitating, obtains Chinese mesona herb polysaccharide solution；
D, CTAB is precipitated: the CTAB solution of equivalent being added in Chinese mesona herb polysaccharide solution, overnight precipitation 8-12h is collected by centrifugation
Precipitating is added 95% ethyl alcohol of 3-5 times of volume, precipitating is collected by centrifugation after 10% NaCl dissolution of precipitating；After distilled water dissolution
The bean jelly grass polysaccharide being further purified；
E, D301 macroreticular resin pre-processes: the obtained Chinese mesona herb polysaccharide solution of upper step being passed through slowly and has been pre-processed
Then D301 macroporous resin column is eluted with the NaCl solution of 0.1-0.3M, flow control is collected in 1.5-2.2mL/min and eluted
Liquid；
F, dialysis removal small molecule: using molecular cut off to dialyse for 10,000 bag filter, remove small-molecule substance,
Chinese mesona herb polysaccharide solution must be refined；
G, vacuum freeze drying: being first placed in 3h at -20 DEG C for Chinese mesona herb polysaccharide sample, is then transferred at -80 DEG C and freezes
Then 3h or more is transferred to vacuum freeze drier drying, obtains the bean jelly grass polysaccharide of purification.
Further, 20h is impregnated with 80% ethyl alcohol of 9 times of volumes after Chinese mesona herb crushes in the step A.
Further, the aqueous solution of 15 times of volumes, extracting in boiling water is added in the step B in pretreated Chinese mesona herb
2h。
Further, the ammonium sulfate that Chinese mesona herb Thick many candies solution is saturated in the step C precipitates 10h at 4 DEG C.
Further, NaCl solution concentration 0.2M in the step E, flow velocity be 1.8mL/min compared with prior art, this
Advantageous effect of the invention is:
The invention discloses it is a kind of with ammonium sulfate and CTAB precipitating combine D301 macroreticular resin purification bean jelly grass polysaccharide and its
Preparation process, it is finally obtaining decoloration using the technique and remove the bean jelly grass polysaccharide of most of albumen and small molecular weight impurity, it obtains
Higher polysaccharide retention rate, percent of decolourization and albumen removal efficiency are obtained, and industrialized scale metaplasia is suitble to produce Chinese mesona herb refined polysaccharide.
Specific embodiment
Technical solution of the present invention is encyclopaedized below with reference to embodiment, but the scope of protection is not limited by this.In embodiment
It is raw materials used all to be obtained from market.
Embodiment 1
The method for combining D301 macroreticular resin purification bean jelly grass polysaccharide using ammonium sulfate and CTAB precipitating, including step is such as
Under:
A, the pretreatment of raw material: impregnating 16h with 60% ethyl alcohol of 8 times of volumes after Chinese mesona herb crushing, and residue shady place air-dries.
B, hot water extraction prepares Thick many candies: the aqueous solution of 10 times of volumes of pretreated Chinese mesona herb addition, extracting in boiling water 1h,
120 mesh filtered through gauze of supernatant, filter residue repeat to extract 1 time, and merging filtrate is concentrated, centrifugation, and the Thick many candies for obtaining Chinese mesona herb are molten
Liquid.
C, ammonium sulfate precipitation: the ammonium sulfate of Chinese mesona herb Thick many candies solution saturation precipitates 8h, polysaccharide and sulphur at 4 DEG C
The volume ratio of acid ammonium solution is 1: 1.It is centrifuged off precipitating, obtains Chinese mesona herb polysaccharide solution.
D, CTAB is precipitated: the CTAB solution of equivalent being added in Chinese mesona herb polysaccharide solution, overnight precipitation 8h, it is heavy to be collected by centrifugation
It forms sediment, after 10% NaCl dissolution of precipitating, 95% ethyl alcohol of 3 times of volumes is added, precipitating is collected by centrifugation.It is obtained after distilled water dissolution
The bean jelly grass polysaccharide being further purified.
E, D301 macroreticular resin pre-processes: the D301 macroreticular resin pre-processed is packed into chromatographic column, ultrapure water 1h
The obtained Chinese mesona herb polysaccharide solution of upper step is passed through slowly into resin column afterwards, then elutes resin with the NaCl solution of 0.1M
Column, flow control collect eluent in 1.5mL/min.
F, dialysis removal small molecule: using molecular cut off to dialyse for 10,000 bag filter, remove small-molecule substance,
Chinese mesona herb polysaccharide solution must be refined.
G, vacuum freeze drying: being first placed in 3h at -20 DEG C for Chinese mesona herb polysaccharide sample, is then transferred at -80 DEG C and freezes
Then 3h or more is transferred to vacuum freeze drier drying, obtains the bean jelly grass polysaccharide of purification.
Embodiment 2
The method for combining D301 macroreticular resin purification bean jelly grass polysaccharide using ammonium sulfate and CTAB precipitating, including step is such as
Under:
A, the pretreatment of raw material: Chinese mesona herb is impregnated for 24 hours after crushing with 80% ethyl alcohol of 9 times of volumes, and residue shady place air-dries.
B, hot water extraction prepares Thick many candies: the aqueous solution of 15 times of volumes of pretreated Chinese mesona herb addition, extracting in boiling water 2h,
120 mesh filtered through gauze of supernatant, filter residue repeat to extract 2 times, and merging filtrate is concentrated, centrifugation, and the Thick many candies for obtaining Chinese mesona herb are molten
Liquid.
C, ammonium sulfate precipitation: the ammonium sulfate of Chinese mesona herb Thick many candies solution saturation precipitates 10h at 4 DEG C, polysaccharide with
The volume ratio of ammonium sulfate is 1: 1.It is centrifuged off precipitating, obtains Chinese mesona herb polysaccharide solution.
D, CTAB is precipitated: the CTAB solution of equivalent being added in Chinese mesona herb polysaccharide solution, overnight precipitation 10h, it is heavy to be collected by centrifugation
It forms sediment, after 10% NaCl dissolution of precipitating, 95% ethyl alcohol of 3 times of volumes is added, precipitating is collected by centrifugation.It is obtained after distilled water dissolution
The bean jelly grass polysaccharide being further purified.
E, D301 macroreticular resin pre-processes: the D301 macroreticular resin pre-processed is packed into chromatographic column, ultrapure water 1h
The obtained Chinese mesona herb polysaccharide solution of upper step is passed through slowly into resin column afterwards, then elutes resin with the NaCl solution of 0.2M
Column, flow control collect eluent in 2.0mL/min.
F, dialysis removal small molecule: using molecular cut off to dialyse for 10,000 bag filter, remove small-molecule substance,
Chinese mesona herb polysaccharide solution must be refined.
G, vacuum freeze drying: being first placed in 3h at -20 DEG C for Chinese mesona herb polysaccharide sample, is then transferred at -80 DEG C and freezes
Then 3h or more is transferred to vacuum freeze drier drying, obtains the bean jelly grass polysaccharide of purification.
Embodiment 3
The method for combining D301 macroreticular resin purification bean jelly grass polysaccharide using ammonium sulfate and CTAB precipitating, including step is such as
Under:
A, the pretreatment of raw material: Chinese mesona herb is impregnated for 24 hours after crushing with 95% ethyl alcohol of 10 times of volumes, residue shady place wind
It is dry.
B, hot water extraction prepares Thick many candies: the aqueous solution of 20 times of volumes of pretreated Chinese mesona herb addition, extracting in boiling water 3h,
120 mesh filtered through gauze of supernatant, filter residue repeat to extract 3 times, and merging filtrate is concentrated, centrifugation, and the Thick many candies for obtaining Chinese mesona herb are molten
Liquid.
C, ammonium sulfate precipitation: the ammonium sulfate of Chinese mesona herb Thick many candies solution saturation precipitates 12h at 4 DEG C, polysaccharide with
The volume ratio of ammonium sulfate is 1: 1.It is centrifuged off precipitating, obtains Chinese mesona herb polysaccharide solution.
D, CTAB is precipitated: the CTAB solution of equivalent being added in Chinese mesona herb polysaccharide solution, overnight precipitation 12h, it is heavy to be collected by centrifugation
It forms sediment, after 10% NaCl dissolution of precipitating, 95% ethyl alcohol of 5 times of volumes is added, precipitating is collected by centrifugation.It is obtained after distilled water dissolution
The bean jelly grass polysaccharide being further purified.
E, D301 macroreticular resin pre-processes: the D301 macroreticular resin pre-processed is packed into chromatographic column, ultrapure water 1h
The obtained Chinese mesona herb polysaccharide solution of upper step is passed through slowly into resin column afterwards, then elutes resin with the NaCl solution of 0.3M
Column, flow control collect eluent in 2.2mL/min.
F, dialysis removal small molecule: using molecular cut off to dialyse for 10,000 bag filter, remove small-molecule substance,
Chinese mesona herb polysaccharide solution must be refined.
G, vacuum freeze drying: being first placed in 3h at -20 DEG C for Chinese mesona herb polysaccharide sample, is then transferred at -80 DEG C and freezes
Then 3h or more is transferred to vacuum freeze drier drying, obtains the bean jelly grass polysaccharide of purification.
Embodiment 4
The method for combining D301 macroreticular resin purification bean jelly grass polysaccharide using ammonium sulfate and CTAB precipitating, including step is such as
Under:
A, the pretreatment of raw material: impregnating 20h with 80% ethyl alcohol of 9 times of volumes after Chinese mesona herb crushing, and residue shady place air-dries.
B, hot water extraction prepares Thick many candies: the aqueous solution of 15 times of volumes of pretreated Chinese mesona herb addition, extracting in boiling water 2h,
120 mesh filtered through gauze of supernatant, filter residue repeat to extract 2 times, and merging filtrate is concentrated, centrifugation, and the Thick many candies for obtaining Chinese mesona herb are molten
Liquid.
C, ammonium sulfate precipitation: the ammonium sulfate of Chinese mesona herb Thick many candies solution saturation precipitates 10h at 4 DEG C, polysaccharide with
The volume ratio of ammonium sulfate is 1: 1.It is centrifuged off precipitating, obtains Chinese mesona herb polysaccharide solution.
D, CTAB is precipitated: the CTAB solution of equivalent being added in Chinese mesona herb polysaccharide solution, overnight precipitation 10h, it is heavy to be collected by centrifugation
It forms sediment, after 10% NaCl dissolution of precipitating, 95% ethyl alcohol of 4 times of volumes is added, precipitating is collected by centrifugation.It is obtained after distilled water dissolution
The bean jelly grass polysaccharide being further purified.
E, D301 macroreticular resin pre-processes: the D301 macroreticular resin pre-processed is packed into chromatographic column, ultrapure water 1h
The obtained Chinese mesona herb polysaccharide solution of upper step is passed through slowly into resin column afterwards, then elutes resin with the NaCl solution of 0.2M
Column, flow control collect eluent in 1.8mL/min.
F, dialysis removal small molecule: using molecular cut off to dialyse for 10,000 bag filter, remove small-molecule substance,
Chinese mesona herb polysaccharide solution must be refined.
G, vacuum freeze drying: being first placed in 3h at -20 DEG C for Chinese mesona herb polysaccharide sample, is then transferred at -80 DEG C and freezes
Then 3h or more is transferred to vacuum freeze drier drying, obtains the bean jelly grass polysaccharide of purification.
Comparative experiments
Comparative example 4 and Sevage method, activated carbon method and hydrogen peroxide method refine Chinese mesona herb Thick many candies, remove with regard to albumen
Rate, pigment removal rate and polysaccharide retention rate etc. are compared, and the results are shown in Table 1.
The measurement of the polyoses content and protein content of gained bean jelly grass polysaccharide and albumen removal efficiency, pigment removal rate and
The calculating of polysaccharide retention rate.Wherein polyoses content (%)=total reducing sugar content (%)-reduced sugar content (%).
Total sugar content measurement uses phend-sulphuric acid；Content of reducing sugar measurement uses DNS method；Determining the protein quantity uses
Bradford method.
In formula: C0、C1Absorbance of the solution at wavelength 450nm respectively before and after resin treatment.
In formula: A0、A1Absorbance of the solution at wavelength 450nm respectively before and after resin treatment.
In formula: M0、M1Polyoses content respectively before and after resin treatment.
The comparison of 1 distinct methods of table purification Chinese mesona herb Thick many candies
| Pigment removal rate % | Albumen removal efficiency % | Polysaccharide retention rate % | Yield % | |
| Embodiment 4 | 90.1 | 91.6 | 75.2 | 70.4 |
| Sevage method | 17.2 | 46.7 | 67.1 | 62.5 |
| Activated carbon method | 75.7 | 14.3 | 43.2 | 50.4 |
| Hydrogen peroxide method | 80.3 | 16.9 | 35.8 | 36.9 |
The present invention combines D301 resin to the method for Chinese mesona herb refinishing polyose using ammonium sulfate and CTAB precipitating, not only can be with
Removing protein is removed, the ability of depigmentation is also had both, therefore the purity of polysaccharide prepared is higher, compared with the Thick many candies before processing
Compared with pigment removal rate is 90.1%, and albumen removal efficiency is 91.6%, and polysaccharide retention rate is 75.2%.
Finally it should be noted that above content is only to structure of the invention example and explanation, affiliated this technology
The technical staff in field does various modifications or supplement to described specific embodiment or is substituted in a similar manner, as long as not
Deviate structure of the invention or beyond the scope defined by this claim, is within the scope of protection of the invention.
Claims (6)
1. the method for combining macroreticular resin purification bean jelly grass polysaccharide using ammonium sulfate and CTAB precipitating, it is characterised in that including following
Step: A. pretreatment of raw material；B. hot water extraction prepares Thick many candies；C. ammonium sulfate precipitation；D.CTAB precipitating；E.D301 macroporous absorption
Resin treatment；F. dialysis removal small molecule；G. vacuum freeze drying obtains purification bean jelly grass polysaccharide.
2. the side according to claim 1 for combining macroreticular resin purification bean jelly grass polysaccharide using ammonium sulfate and CTAB precipitating
Method, it is characterized in that:
A, pretreatment of raw material: 16-24h, residue shady place wind are impregnated with the 60-95% ethyl alcohol of 8-10 times of volume after Chinese mesona herb crushing
It is dry；
B, hot water extraction prepares Thick many candies: the aqueous solution of 10-20 times of volume, extracting in boiling water 1- is added in pretreated Chinese mesona herb
3h, 120 mesh filtered through gauze of supernatant, filter residue repeat to extract 1-3 times, and merging filtrate is concentrated, and it is slightly more to obtain Chinese mesona herb for centrifugation
Sugar juice；
C, ammonium sulfate precipitation: the ammonium sulfate of Chinese mesona herb Thick many candies solution saturation precipitates 8-12h at 4 DEG C, Thick many candies with
The volume ratio of ammonium sulfate is 1: 1；It is centrifuged off precipitating, obtains Chinese mesona herb polysaccharide solution；
D, CTAB is precipitated: the CTAB solution of equivalent is added in Chinese mesona herb polysaccharide solution, precipitating is collected by centrifugation in overnight precipitation 8-12h,
After 10% NaCl dissolution of precipitating, 95% ethyl alcohol of 3-5 times of volume is added, precipitating is collected by centrifugation；It is obtained after distilled water dissolution
The bean jelly grass polysaccharide being further purified；
E, D301 macroreticular resin pre-processes: the obtained Chinese mesona herb polysaccharide solution of upper step is passed through slowly the D301 pre-processed
Then macroporous resin column is eluted with the NaCl solution of 0.1-0.3M, flow control collects eluent in 1.5-2.2mL/min；
F, it dialysis removal small molecule: uses molecular cut off to dialyse for 10,000 bag filter, removes small-molecule substance, obtain smart
Chinese mesona herb polysaccharide solution processed；
G, vacuum freeze drying: being first placed in 3h at -20 DEG C for Chinese mesona herb polysaccharide sample, be then transferred at -80 DEG C freeze 3h with
On, it is then transferred to vacuum freeze drier drying, obtains the bean jelly grass polysaccharide of purification.
3. the side according to claim 2 for combining macroreticular resin purification bean jelly grass polysaccharide using ammonium sulfate and CTAB precipitating
Method, characterized in that impregnate 20h with 80% ethyl alcohol of 9 times of volumes after Chinese mesona herb crushes in the step A.
4. the side according to claim 2 for combining macroreticular resin purification bean jelly grass polysaccharide using ammonium sulfate and CTAB precipitating
Method, it is characterized in that: in the step B pretreated Chinese mesona herb be added in 15 times of volumes aqueous solution, extracting in boiling water 2h.
5. the side according to claim 2 for combining macroreticular resin purification bean jelly grass polysaccharide using ammonium sulfate and CTAB precipitating
Method, it is characterized in that: the ammonium sulfate that Chinese mesona herb Thick many candies solution is saturated in the step C precipitates 10h at 4 DEG C.
6. the side according to claim 2 for combining macroreticular resin purification bean jelly grass polysaccharide using ammonium sulfate and CTAB precipitating
Method, it is characterized in that: NaCl solution concentration 0.2M, flow velocity 1.8mL/min in the step E.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910278506.1A CN110054704B (en) | 2019-04-08 | 2019-04-08 | Method for refining mesona chinensis benth polysaccharide by combining ammonium sulfate and CTAB (cetyl trimethyl ammonium bromide) precipitation with macroporous resin |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910278506.1A CN110054704B (en) | 2019-04-08 | 2019-04-08 | Method for refining mesona chinensis benth polysaccharide by combining ammonium sulfate and CTAB (cetyl trimethyl ammonium bromide) precipitation with macroporous resin |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN110054704A true CN110054704A (en) | 2019-07-26 |
| CN110054704B CN110054704B (en) | 2020-07-14 |
Family
ID=67318466
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910278506.1A Active CN110054704B (en) | 2019-04-08 | 2019-04-08 | Method for refining mesona chinensis benth polysaccharide by combining ammonium sulfate and CTAB (cetyl trimethyl ammonium bromide) precipitation with macroporous resin |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110054704B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115521387A (en) * | 2022-09-30 | 2022-12-27 | 中国热带农业科学院热带作物品种资源研究所 | Acidic polysaccharide and method for separating acidic polysaccharide from basil seed gum |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102911282A (en) * | 2012-11-02 | 2013-02-06 | 龙岩嘉麒生物科技有限公司 | Decolorization and deproteinization process of mesona chinensis benth polysaccharide solution |
| CN102924621A (en) * | 2012-11-16 | 2013-02-13 | 上海应用技术学院 | Mesona chinensis benth polysaccharide and preparation method and applications thereof |
| CN104045725A (en) * | 2014-06-25 | 2014-09-17 | 聊城大学 | Method for refining inonotus obliquus crude polysaccharide by adopting D301G anion resin |
-
2019
- 2019-04-08 CN CN201910278506.1A patent/CN110054704B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102911282A (en) * | 2012-11-02 | 2013-02-06 | 龙岩嘉麒生物科技有限公司 | Decolorization and deproteinization process of mesona chinensis benth polysaccharide solution |
| CN102924621A (en) * | 2012-11-16 | 2013-02-13 | 上海应用技术学院 | Mesona chinensis benth polysaccharide and preparation method and applications thereof |
| CN104045725A (en) * | 2014-06-25 | 2014-09-17 | 聊城大学 | Method for refining inonotus obliquus crude polysaccharide by adopting D301G anion resin |
Non-Patent Citations (5)
| Title |
|---|
| TAO FENG等: "In vitro and in vivo antioxidative and radioprotective capacities of polysaccharide isolated from Mesona Blumes gum", 《STARCH/STARKE》 * |
| WENJIEWANG等: "Gel properties and interactions of Mesona blumes polysaccharide-soy protein isolates mixed gel: The effect of salt addition", 《CARBOHYDRATE POLYMERS》 * |
| 张恒: "《应用生物化学》", 31 January 2013, 中国矿业大学出版社 * |
| 林少琴 等: "仙草多糖的分离纯化及鉴定", 《天然产物研究与开发》 * |
| 陈荔红 等: "仙草胶提取液脱色工艺的研究", 《中国农学通报》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115521387A (en) * | 2022-09-30 | 2022-12-27 | 中国热带农业科学院热带作物品种资源研究所 | Acidic polysaccharide and method for separating acidic polysaccharide from basil seed gum |
| CN115521387B (en) * | 2022-09-30 | 2023-10-13 | 中国热带农业科学院热带作物品种资源研究所 | Acidic polysaccharide and method for separating acidic polysaccharide from basil seed gum |
Also Published As
| Publication number | Publication date |
|---|---|
| CN110054704B (en) | 2020-07-14 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101209284A (en) | Method for continuously extracting alkaloids, flavone and polysaccharide active components from mulberry leaves | |
| CN101744886B (en) | Method for extracting high-purity schisandra total lignan | |
| CN101914166A (en) | Technical method for decoloring and deproteinizing corn stigma polysaccharide | |
| CN106349324B (en) | The method of extraction separation crataegolic acid from olive growing leaves | |
| CN105053952B (en) | A kind of processing technology of the dried orange peel extracts of no bitter taste | |
| CN104262446B (en) | The method of mogroside Ⅴ is extracted from Grosvenor Momordica | |
| CN102643315A (en) | Method for purifying phlorizin from apple velamen | |
| CN110054704A (en) | The method that macroreticular resin purification bean jelly grass polysaccharide is combined using ammonium sulfate and CTAB precipitating | |
| CN102180985A (en) | Method for extracting and purifying mugwort polysaccharides | |
| CN101638404B (en) | High-purity salvianolic acid B and preparation method and application thereof | |
| CN106947000A (en) | A kind of preparation method of high-purity polyfructosan | |
| CN105878518A (en) | Separation and purification method of litchi polyphenol | |
| CN102648965A (en) | Industrialization preparation method of lophatherum gracile general flavone | |
| CN113913029B (en) | Method for preparing effective components of gardenia jasminoides ellis | |
| CN106336440B (en) | The method of extraction separation oleanolic acid from olive growing leaves | |
| CN106860489A (en) | A kind of extracting method of myrica rubra leaf polyphenol | |
| CN104497163A (en) | Korla pear polysaccharide extracting method and method for applying korla pear polysaccharide to cigarettes | |
| CN105906738B (en) | The method of separating polyose is extracted in a kind of cockscomb | |
| CN108250052A (en) | The method of separation and Extraction quebrachite from sapindaceous plant longan or lichee | |
| CN108126000A (en) | Arasaponin extracts preparation method in fresh Radix Notoginseng | |
| CN109021042B (en) | Method for extracting high-purity oleuropein from olive leaves | |
| CN107582582A (en) | A kind of technique of extraction purification Moringa flavones | |
| CN102771850B (en) | Method for preparing cooling drink by use of chrysanthemum straw | |
| CN107474086A (en) | The technique for extracting stevioside | |
| CN102002114B (en) | Gallnut polysaccharide composite and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |