CN110042169A - A kind of T. grubii group specific molecular marker primer, kit and identification method - Google Patents
A kind of T. grubii group specific molecular marker primer, kit and identification method Download PDFInfo
- Publication number
- CN110042169A CN110042169A CN201910472846.8A CN201910472846A CN110042169A CN 110042169 A CN110042169 A CN 110042169A CN 201910472846 A CN201910472846 A CN 201910472846A CN 110042169 A CN110042169 A CN 110042169A
- Authority
- CN
- China
- Prior art keywords
- group
- primer
- grubii
- river
- pcr amplification
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/156—Polymorphic or mutational markers
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The invention discloses a kind of T. grubii group specific molecular marker primer, kit and identification method, primer ThtC1010, ThtC1029, ThtC1034；Wherein, ThtC1010 primer sequence is as shown in SEQ ID NO.1-2, and ThtC1029 primer sequence is as shown in SEQ ID NO.3-4, and ThtC1034 primer sequence is as shown in SEQ ID NO.5-6.The present invention is the new method of the differentiation T. grubii group established for the first time based on Protocols in Molecular Biology principle both at home and abroad, it is on the basis of T. grubii EST data, successfully develop EST-SSR label, and the genetic background of the group of T. grubii is analyzed with part label, have found the specific marker that can distinguish 3 groups, wherein the site THC1010 and the site THC1029 can accurately distinguish Gen He group and other Liang Ge groups, the site THC1034 can distinguish three groups, with accuracy height, technique reproducible is good, the high advantage of cost performance.
Description
Technical field
The present invention relates to a kind of T. grubii group specific molecular marker primer, kit and identification methods, belong to
Technical field of molecular biology.
Background technique
T. grubii Thymallus arcticus grubei (Dybowski) category salmon shape mesh (Salmoniformes),
Grayling section (Thymallidae), grayling category (Thymallus), are commonly called as: spot trout, red squama fish are distributed in Eurasia, at me
For state's NATURAL DISTRIBUTION in the tributary of Heilongjiang river, fine and tender taste, delicious flavour are that Heillongjiang River system is distinctive rare cold aqueous
Fish.The fish requires harshness to environmental condition, is hardly formed large-scale groups in its natural state, can not form certain catch
Fishing amount.Simultaneously because the variation of environmental condition, habitat fisherman catches and builds reservoir and water conservancy projects in the migration phase, changes
The oviposition of T. grubii is become, has ingested, overwintering environment, it is bred and existence causes extreme influence, leads to wild resource
It is exhausted.T. grubii artificial propagation at present has been successful, and artificial fecundation, which releases, gradually to be carried out.Fish population divides
Fish germ plasma resource is protected and manages significant, only understands the genetic background and resource of T. grubii population in depth
Situation understands fully group source, could preferably protect T. grubii, carries out T. grubii breeding and resource repair.
Molecular labeling has a wide range of applications in Germplasm Resources Diversity research.The polymorphism of DNA level mainly shows
In the difference of nucleotide sequence, the difference of any kind of nucleotide sequence can carry out it with molecular marking technique
Detection.Wherein simple repeated sequence (Simple Sequence Repeats, SSR) is also referred to as microsatellite DNA, usually by 1~
6 nucleotide are the tandem sequence repeats DNA sequence dna of recurring unit, have quantity more, polymorphism height are disclosed, in spies such as codominant inheritances
Point has been widely used in the research such as the assignment of genes gene mapping, fingerprint map construction, genetic map construction and germplasm identification.This hair
Bright library EST (Express sequence tag, EST) that T. grubii is obtained by high-flux sequence, is excavated
The EST-SSR of T. grubii is marked, and analyzes the genetic background of different groups T. grubii, it is distinctive to find different groups
EST-SSR label provides basic data for further analysis, Germplasm Identification and molecular marker assisted selection breeding etc..
Summary of the invention
For the discrimination method for lacking T. grubii group, the object of the present invention is to provide a kind of T. grubii groups
Specific molecular marker primer, kit and identification method can accurately distinguish the T. grubii group in different basins.
To achieve the goals above, the technical scheme adopted by the invention is that:
A kind of T. grubii group specific molecular marker primer, primer ThtC1010, ThtC1029,
ThtC1034；Wherein, the upstream primer sequence of ThtC1010 is as shown in SEQ ID NO.1, downstream primer sequence such as SEQ ID
Shown in NO.2, the upstream primer sequence of ThtC1029 is as shown in SEQ ID NO.3, downstream primer sequence such as SEQ ID NO.4 institute
Show, the upstream primer sequence of ThtC1034 is as shown in SEQ ID NO.5, and downstream primer sequence is as shown in SEQ ID NO.6.
A kind of kit of T. grubii group identification, including primer ThtC1010, ThtC1029, ThtC1034.
Mentioned reagent box further includes 2 × Taq PCR Mix and ultrapure water.
A kind of identification method for T. grubii group, comprising the following steps:
(1) sample collection: the grayling sample in acquisition Heilungkiang difference basin；
(2) grayling sample DNA extracting genome DNA: is extracted using phenol chloroform method；
(3) PCR amplification: using the genomic DNA of extraction as template, PCR amplification is carried out using the primer of claim 1；
(4) electrophoresis dying compare map: PCR after reaction, takes PCR product electrophoresis detection, according to electrophoresis result with mention
The standard diagram of confession compares, and is identified.
The reaction system of PCR amplification is 20 μ l, each ingredient are as follows: 2 × Taq PCR Mix, 10 μ l, 50ng/ μ l genomic DNA
2 μ l, each 0.5 μ l of 10 μM of upstream and downstream primers, supply 20 μ l with ultrapure water.
The response procedures of PCR amplification are as follows: 95 DEG C of initial denaturation 3min；95 DEG C of denaturation 30s, 60 DEG C of annealing 30s, 72 DEG C extend
30s, 25 circulations；72 DEG C of last extension 5min.
Different basins are root river, Tao'erhe River and the Wusuli River.
In standard diagram,
The pcr amplification product of primer ThtC1010 is Gen He group for A band；Pcr amplification product is wash one's face for B band
Er He group and/or Wusuli River group；
The pcr amplification product of primer ThtC1029 is Gen He group for C band；Pcr amplification product is wash one's face for D band
Er He group and/or Wusuli River group；
The pcr amplification product of primer ThtC1034 be in E, F and G band 1 or 2 be Gen He group；PCR amplification produces
Object is Tao'erhe River group for H band；It is Wusuli River group that pcr amplification product, which is I band or I and H band,.
A kind of application of above-mentioned primer in the identification of T. grubii group germplasm.
A kind of application of above-mentioned primer in the molecular marker assisted selection breeding of T. grubii group.
The invention has the advantages that:
The present invention is the new of the differentiation T. grubii group established for the first time based on Protocols in Molecular Biology principle both at home and abroad
Method is to successfully develop EST-SSR label, and with part label to Heilungkiang on the basis of T. grubii EST data
The genetic background of the group of grayling is analyzed, and the specific marker that can distinguish 3 groups is had found, and wherein THC1010
Point and the site THC1029 can accurately distinguish Gen He group and other Liang Ge groups, have A (109bp), C (198bp) band
Individual is Gen He group, and the individual with B (114bp), D (184bp) band is Tao'erhe River group and Wusuli River group；
The site THC1034 can distinguish three groups, with 1 or 2 in any E (174bp), F (168bp) and G (164bp) band
A individual is Gen He group, and only having H (152bp) band is Tao'erhe River group, and Wusuli River group is in addition to I
Outside (156bp) band, partial mass also has H band.Wherein, THC1034 pair of primers can be by Gen He group, Tao'erhe River group
Body and Wusuli River group distinguish, and have accuracy high, and technique reproducible is good, the high advantage of cost performance, can be simultaneously to big
Amount sample is detected, and the complexity of T. grubii germ plasm resource and group's judge is reduced.
Detailed description of the invention
Fig. 1 is THC1010 primer amplification map.
Fig. 2 is THC1029 primer amplification map.
Fig. 3 is THC1034 primer amplification map.
Specific embodiment
Specific embodiments of the present invention will be described in further detail with reference to embodiments.
Embodiment 1
1, design of primers
T. grubii RNA is sequenced, the library EST of T. grubii is obtained, in the library EST search have 2,3,4,
The microsatellite locus of 5 repetitive sequences.50 sequences are randomly selected, with 3 design primer of Primer, primer length 19-25bp, GC
Content 50-60%.There is group's specificity to 50 pairs of primer screening discovery ThtC1010, ThtC1029 and ThtC1034 labels.
T. grubii group specific molecular marker primer sequence:
ThtC1010 upstream primer: 5 '-TCAGGTTGTGCAAAATCTCGA-3 ' (SEQ ID NO.1)
Downstream primer: 5 '-GGCCAGCAGAACCAGAATCA-3 ' (SEQ ID NO.2)
ThtC1029 upstream primer: 5 '-GTGTTTCTTCCTGGCAGGGA-3 ' (SEQ ID NO.3)
Downstream primer: 5 '-TGGTGGTTAAGACTCAGAGACC-3 ' (SEQ ID NO.4)
ThtC1034 upstream primer: 5 '-TGGTGACACTGAAGAGGGGA-3 ' (SEQ ID NO.5)
Downstream primer: 5 '-AGCGTGTTATTACCAGTGCT-3 ' (SEQ ID NO.6)
2, sample collection
The wild T. grubii of acquisition Heillongjiang River system preserved egg Jiangxi source Nenjiang right bank maximum tributary Tao'erhe River, black dragon respectively
The wild T. grubii in one of maximum tributary in river Eergounahe River root river is wild black with the tributary Wusuli River, Chinese Heilungkiang
Longjiang grayling fin ray sample is saved with 95% (v/v) ethyl alcohol.
3, extracting genome DNA
The T. grubii DNA of above-mentioned different groups is extracted using phenol chloroform method, dilution 50ng/ μ l is spare.
4, pcr amplification reaction
Reaction system is 20 μ l, each ingredient are as follows: 2 × Taq PCR Mix, 10 μ l, 2 μ l of 50ng/ μ l genomic DNA, 10 μM
Each 0.5 μ l of upstream and downstream primer supplies 20 μ l with ultrapure water.
Response procedures are as follows: 95 DEG C of initial denaturation 3min；95 DEG C of denaturation 30s, 60 DEG C of annealing 30s, 72 DEG C of extension 30s, 25 are followed
Ring；72 DEG C of last extension 5min.
5, electrophoresis dying compares map
PCR after reaction, take 1.5 μ l PCR products with 8% non denatured polyacrylate hydrogel electrophoresis detection, 220V, electricity
Swim 3h, and silver staining photographs to record.It is compared, is identified according to electrophoresis result and the standard diagram of offer.In standard diagram,
The pcr amplification product of primer ThtC1010 is Gen He group for A band (109bp's)；Pcr amplification product is B item
Band (114bp) is Tao'erhe River group and/or Wusuli River group (Fig. 1)；
The pcr amplification product of primer ThtC1029 is Gen He group for C band (198bp's)；Pcr amplification product is D item
Band (184bp) is Tao'erhe River group and/or Wusuli River group (Fig. 2)；
The pcr amplification product of primer ThtC1034 is E (174bp), 1 or 2 in F (168bp) and G (164bp) band
Be Gen He group；Pcr amplification product is Tao'erhe River group for H band (152bp's)；Pcr amplification product is I band
(156bp) or I and H band are Wusuli River group (Fig. 3).
Embodiment 2
The fin ray sample for acquiring 20 tail of root river grayling, 26 tail of 20 tail of Tao'erhe River grayling and Wusuli River grayling, utilizes implementation
The identification method of example 1, is identified.It is compared according to electrophoresis result and the standard diagram of offer, finds primer ThtC1010 root river
The amplified band size 109bp identical as A of 20 tail grayling of group, 26 tail fennel of 20 tail grayling of Tao'erhe River group and Wusuli River group
The amplified band size of fish 114bp identical as B；The amplified band size and C phase of 20 tail grayling of primer ThtC1029 Gen He group
Same 198bp, the amplified band size 184bp identical as D of 26 tail grayling of 20 tail grayling of Tao'erhe River group and Wusuli River group；Draw
Object ThtC1034 Gen He group amplified band size identical as E, F and G is respectively 174bp, 168bp and 164bp, wherein 5 tails have
There is E band, 4 tails have E and F band, and 5 tails have F band, and remaining 6 tails have F and G band, 20 tail grayling of Tao'erhe River group
Amplified band size 152bp identical as H, 26 tail grayling of Wusuli River group all has I band 156bp, wherein 10 tail graylings are same
When there are H band 152bp.Primer ThtC1010 and primer ThtC1029 can be by Gen He group and other Liang Ge groups, accuracy
Up to 100%；Primer ThtC1034 can distinguish three groups, accuracy 100%.
Sequence table
<110>Heilongjiang Inst. of Aquatic Products, Chinese Academy of Aquatic Products Scie
<120>a kind of T. grubii group specific molecular marker primer, kit and identification method
<160> 6
<170> SIPOSequenceListing 1.0
<210> 1
<211> 21
<212> DNA
<213>artificial sequence ()
<400> 1
tcaggttgtg caaaatctcg a 21
<210> 2
<211> 20
<212> DNA
<213>artificial sequence ()
<400> 2
ggccagcaga accagaatca 20
<210> 3
<211> 20
<212> DNA
<213>artificial sequence ()
<400> 3
gtgtttcttc ctggcaggga 20
<210> 4
<211> 22
<212> DNA
<213>artificial sequence ()
<400> 4
tggtggttaa gactcagaga cc 22
<210> 5
<211> 20
<212> DNA
<213>artificial sequence ()
<400> 5
tggtgacact gaagagggga 20
<210> 6
<211> 20
<212> DNA
<213>artificial sequence ()
<400> 6
agcgtgttat taccagtgct 20
Claims (10)
1. a kind of T. grubii group specific molecular marker primer, which is characterized in that primer ThtC1010,
ThtC1029,ThtC1034；Wherein, the upstream primer sequence of ThtC1010 is as shown in SEQ ID NO.1, and downstream primer sequence is such as
Shown in SEQ ID NO.2, the upstream primer sequence of ThtC1029 is as shown in SEQ ID NO.3, downstream primer sequence such as SEQ ID
Shown in NO.4, the upstream primer sequence of ThtC1034 is as shown in SEQ ID NO.5, downstream primer sequence such as SEQ ID NO.6 institute
Show.
2. a kind of kit of T. grubii group identification, which is characterized in that including primer described in claim 1.
3. kit according to claim 2, which is characterized in that further include 2 × Taq PCR Mix and ultrapure water.
4. a kind of identification method for T. grubii group, which comprises the following steps:
(1) sample collection: the grayling sample in acquisition Heilungkiang difference basin；
(2) grayling sample DNA extracting genome DNA: is extracted using phenol chloroform method；
(3) PCR amplification: using the genomic DNA of extraction as template, PCR amplification is carried out using the primer of claim 1；
(4) electrophoresis dying compares map: PCR after reaction, takes PCR product electrophoresis detection, according to electrophoresis result and offer
Standard diagram comparison, is identified.
5. identification method according to claim 4, which is characterized in that the reaction system of PCR amplification is 20 μ l, each ingredient
Are as follows: 2 × Taq PCR Mix, 10 μ l, 2 μ l of 50ng/ μ l genomic DNA, each 0.5 μ l of 10 μM of upstream and downstream primers are mended with ultrapure water
20 μ l of foot.
6. identification method according to claim 4, which is characterized in that the response procedures of PCR amplification are as follows: 95 DEG C of initial denaturations
3min；95 DEG C of denaturation 30s, 60 DEG C of annealing 30s, 72 DEG C of extension 30s, 25 recycle；72 DEG C of last extension 5min.
7. identification method according to claim 4, which is characterized in that different basins are root river, Tao'erhe River and the Wusuli River.
8. identification method according to claim 7, which is characterized in that in standard diagram,
The pcr amplification product of primer ThtC1010 is Gen He group for A band；Pcr amplification product is Tao'erhe River for B band
Group and/or Wusuli River group；
The pcr amplification product of primer ThtC1029 is Gen He group for C band；Pcr amplification product is Tao'erhe River for D band
Group and/or Wusuli River group；
The pcr amplification product of primer ThtC1034 be in E, F and G band 1 or 2 be Gen He group；Pcr amplification product is
H band is Tao'erhe River group；It is Wusuli River group that pcr amplification product, which is I band or I and H band,.
9. a kind of application of primer as described in claim 1 in the identification of T. grubii group germplasm.
10. a kind of application of primer as described in claim 1 in the molecular marker assisted selection breeding of T. grubii group.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910472846.8A CN110042169B (en) | 2019-05-31 | 2019-05-31 | Molecular marker primer, kit and identification method for group specificity of Fennel fish in Heilongjiang |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910472846.8A CN110042169B (en) | 2019-05-31 | 2019-05-31 | Molecular marker primer, kit and identification method for group specificity of Fennel fish in Heilongjiang |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN110042169A true CN110042169A (en) | 2019-07-23 |
| CN110042169B CN110042169B (en) | 2022-11-18 |
Family
ID=67284419
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910472846.8A Active CN110042169B (en) | 2019-05-31 | 2019-05-31 | Molecular marker primer, kit and identification method for group specificity of Fennel fish in Heilongjiang |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110042169B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112813172A (en) * | 2021-03-04 | 2021-05-18 | 中国水产科学研究院黑龙江水产研究所 | Primer and method for identifying zander population |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2009031740A1 (en) * | 2007-09-04 | 2009-03-12 | Korea Research Institute Of Bioscience And Biotechnology | Functional data integrated genetic marker system, ssr-fmm, and improved genetic research and marker assisted selection with ssr-fmm |
| CN102251048A (en) * | 2011-08-05 | 2011-11-23 | 中国水产科学研究院珠江水产研究所 | Method for identifying grass carps by using microsatellite primers |
| CN102876777A (en) * | 2011-07-11 | 2013-01-16 | 浙江海洋学院 | Specific primer and screening method of brown croaker EST (Expressed Sequence Tag) microsatellite markers |
| CN105779614A (en) * | 2016-04-19 | 2016-07-20 | 新疆大学 | Sinkiang Alaska grayling heritable variation detection method utilizing microsatellite marker Thymalag |
| CN108277287A (en) * | 2018-04-18 | 2018-07-13 | 中国水产科学研究院黑龙江水产研究所 | A kind of molecular labeling, kit and differentiating method for distinguishing Heilungkiang triangular bream and southern triangular bream category fish |
-
2019
- 2019-05-31 CN CN201910472846.8A patent/CN110042169B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2009031740A1 (en) * | 2007-09-04 | 2009-03-12 | Korea Research Institute Of Bioscience And Biotechnology | Functional data integrated genetic marker system, ssr-fmm, and improved genetic research and marker assisted selection with ssr-fmm |
| CN102876777A (en) * | 2011-07-11 | 2013-01-16 | 浙江海洋学院 | Specific primer and screening method of brown croaker EST (Expressed Sequence Tag) microsatellite markers |
| CN102251048A (en) * | 2011-08-05 | 2011-11-23 | 中国水产科学研究院珠江水产研究所 | Method for identifying grass carps by using microsatellite primers |
| CN105779614A (en) * | 2016-04-19 | 2016-07-20 | 新疆大学 | Sinkiang Alaska grayling heritable variation detection method utilizing microsatellite marker Thymalag |
| CN108277287A (en) * | 2018-04-18 | 2018-07-13 | 中国水产科学研究院黑龙江水产研究所 | A kind of molecular labeling, kit and differentiating method for distinguishing Heilungkiang triangular bream and southern triangular bream category fish |
Non-Patent Citations (3)
| Title |
|---|
| C. WANG，等: "Isolation of Polymorphic RAPD-SSR Markersfrom Xinjiang Arctic Grayling (Thymallus arcticus grubei)and a Test of Cross-Species Amplification", 《RUSSIAN JOURNAL OF GENETICS》 * |
| 孙家贤，等: "呼玛河3种茴鱼遗传分化及属内地位的微卫星分析", 《水产学杂志》 * |
| 马波，等: "下游黑龙江茴鱼种群遗传变异及地理分化的微卫星分析", 《中国水产科学》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112813172A (en) * | 2021-03-04 | 2021-05-18 | 中国水产科学研究院黑龙江水产研究所 | Primer and method for identifying zander population |
| CN112813172B (en) * | 2021-03-04 | 2021-07-13 | 中国水产科学研究院黑龙江水产研究所 | Primer and method for identifying zander population |
Also Published As
| Publication number | Publication date |
|---|---|
| CN110042169B (en) | 2022-11-18 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103387980B (en) | A kind of Portunus trituberculatus Miers microsatellite locus and primer | |
| CN113502333B (en) | Molecular marker C42257 for rapidly identifying genetic sex of penaeus japonicus and application thereof | |
| CN105603098B (en) | For the microsatellite marker primer and identification method of Penaeus monodon microsatellite Parentage determination and application | |
| CN109554488A (en) | Female Amur Sturgeon specific DNA fragment and application | |
| CN108998547A (en) | A kind of microsatellite marking method for C. guichenoti paternity test | |
| CN106520939B (en) | A kind of method and its application of madai Germplasm Identification | |
| CN104561355B (en) | Multiplex-PCR method for parentage assignment of scapharca broughtonii | |
| CN112226518B (en) | Molecular marker C69483 for rapidly identifying genetic sex of red swamp crayfish and application thereof | |
| CN109554486A (en) | SNP marker relevant to grass carp character and its application | |
| CN105483227B (en) | A kind of China's dace DNA bar code standard detection gene and its application | |
| CN105349691B (en) | It is a kind of identify Crustin genetic sex DNA sequence tag and its application | |
| CN109880893A (en) | DNA fragment specific and application for mystus nemurus sex identification | |
| CN112226497B (en) | Molecular marker C98284 for rapidly identifying genetic sex of red swamp crayfish and application thereof | |
| CN110042169A (en) | A kind of T. grubii group specific molecular marker primer, kit and identification method | |
| CN102912017A (en) | Method for quickly and accurately identifying northern snakehead, Taiwan snakehead and hybrid snakehead | |
| CN109536624B (en) | Fluorescent molecular marker and detection method for discriminating true and false male fish of cynoglossus semilaevis | |
| CN114574600B (en) | Male molecular marker of alosa sapidissima, and specific primer pair and application thereof | |
| CN110452992A (en) | A kind of labeling method of litopenaeus vannamei EST microsatellite locus primer | |
| CN113502334B (en) | Molecular marker C27449 for rapidly identifying genetic sex of Penaeus japonicus and application thereof | |
| CN109234412A (en) | The quickly method of the fast erythroculter ilishaeformis of the detection speed of growth and molecular labeling used | |
| CN110951892B (en) | SSR primer pair group for identifying several sturgeon species, kit, identification method and application | |
| JP4982746B2 (en) | Pig parent-child determination method using DNA marker | |
| CN108410963A (en) | A kind of long Qi Wen Minnow paternity test methods based on microsatellite Multiplex fluorescent PCR | |
| KR102074139B1 (en) | Genetic markers and methods for identifying species belong to Cobitidae | |
| CN105755165B (en) | A kind of the genotype detection primer and method of lefteye flounder microsatellite marker Paraoliva-2 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |