CN110038056A - A kind of compound Chinese Gall ointment and preparation method thereof for treating pet limbs inflammatory swelling - Google Patents
A kind of compound Chinese Gall ointment and preparation method thereof for treating pet limbs inflammatory swelling Download PDFInfo
- Publication number
- CN110038056A CN110038056A CN201910454496.2A CN201910454496A CN110038056A CN 110038056 A CN110038056 A CN 110038056A CN 201910454496 A CN201910454496 A CN 201910454496A CN 110038056 A CN110038056 A CN 110038056A
- Authority
- CN
- China
- Prior art keywords
- ointment
- chinese gall
- group
- compound chinese
- skin
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/56—Materials from animals other than mammals
- A61K35/63—Arthropods
- A61K35/64—Insects, e.g. bees, wasps or fleas
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/26—Aristolochiaceae (Birthwort family), e.g. heartleaf
- A61K36/268—Asarum (wild ginger)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/32—Burseraceae (Frankincense family)
- A61K36/324—Boswellia, e.g. frankincense
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/32—Burseraceae (Frankincense family)
- A61K36/328—Commiphora, e.g. mecca myrrh or balm of Gilead
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/75—Rutaceae (Rue family)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/0012—Galenical forms characterised by the site of application
- A61K9/0014—Skin, i.e. galenical aspects of topical compositions
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/06—Ointments; Bases therefor; Other semi-solid forms, e.g. creams, sticks, gels
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P29/00—Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P7/00—Drugs for disorders of the blood or the extracellular fluid
- A61P7/10—Antioedematous agents; Diuretics
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
Abstract
The invention discloses a kind of compound Chinese Gall ointment and preparation method thereof for treating pet limbs inflammatory swelling, are related to the field of Chinese medicines, bulk pharmaceutical chemicals are made of Chinese gall, asarum, cortex dictamni, olibanum, myrrh and borneol；By taking each taste Chinese medicine, it is first ground into coarse powder, mixes, mixture is made；It takes 100g mixture to be put into the distilled water of 1000mL to impregnate, after mild and heavy respiration distinguishes infusion, the crude drug liquid that concentration is 1g/mL is made；Albolene, glycerin monostearate, stearic acid and atoleine is taken to be heated to melting as oily phase；Crude drug liquid, triethanolamine and ethylparaben are separately dissolved as water phase, then water phase is added in oily phase, is removed from water-bath, transdermal agent borneol is added, and be stirred at room temperature, ointment is both obtained after condensation；The effective anti-inflammatory detumescence of ointment prepared by the present invention, inhibition of pain do not generate drug resistance, no allergic reaction, and non-stimulated and toxic side effect can effectively treat the common inflammatory swelling as caused by various disease in pet diagnosis and treatment.
Description
Technical field
The present invention relates to tcm field more particularly to a kind of anti-inflammatory and antalgic, inhibition granuloma hyperplasia, pet is examined
The common medicative ointment of pet inflammatory swelling as caused by various disease in treatment.
Background technique
Inflammation be in vascular system living tissue to damage factor and body to defense reaction caused by stimulating.Pain is
Inflammation proceeds to the pathological manifestations of certain phase.Improve with the development of the times, for inflammation and pain understanding also increasingly
Deeply, more extensive for treating the types of drugs used, however hormone ointment and the long-time service of anti-inflammatory and antalgic ointment can make
At some side effects.
Traditional Chinese herb medicine directly acts on skin, and avoiding a series of adverse reactions, Chinese medicine caused by oral drugs also has
The advantages such as cheap, safe and reliable, easy to spread.But passes through much the study found that being also no lack of in Chinese medicine and cause malicious pair
Reaction, such as will appear the adverse reaction based on organ injury after achene of Siberian cocklebur medication, wherein the damage of kidney and liver is the most significant.
Lack of standardization use of many Chinese medicines can all cause lesions of liver and kidney, such as tripterygium wilfordii, tripterygium hypoglaucum hutcs, Cortex Magnoliae Officinalis, Ilex Latifolia Thunb, Coriaria sinica fruit
Adverse reaction can exactly cause kidney injury, and airpotato yam, chrysanthemum Radix Notoginseng, fleece-flower root etc. can then cause hepar damnification.
Trouble, which is doted on, in pet diagnosis and treatment, caused by unknown cause subcutaneously there is part-circular tubercle, cellulitis, presses pain
Non- open inflammatory infection, clinically mostly treated using hormone ointment or anti-inflammatory analgesic ointment, because of agents area right and wrong
Opening, the anti-inflammatory and analgesic effect that the common ointment that veterinary clinic discloses application penetrates skin arrival site is unobvious, causes to control
Therapeutic effect difference and drug service life extend, and increase the possibility for the treatment of cost and drug resistance.
Summary of the invention
Answering for pet limbs inflammatory swelling is treated it is an object of the invention to overcome the deficiencies of the prior art and provide a kind of
Square Chinese gall ointment and preparation method thereof, to solve to produce using part Chinese medicine treatment pet limbs inflammatory swelling in the prior art
The problems such as raw side effect.
The present invention is achieved by the following technical solutions:
The present invention provides a kind of Chinese gall ointment treated and dote on inflammatory swelling, the composition packet of ointment component by weight
It includes:
27 parts of Chinese gall
21 parts of asarum
24 parts of cortex dictamni
12 parts of olibanum
16 parts of myrrh
1.5 parts of borneol.
The present invention also provides a kind of method for preparing above-mentioned ointment, this method specifically:
Chinese gall 27g, asarum 21g, cortex dictamni 24g, olibanum 12g, myrrh 16g are weighed, powdery is then comminuted into, is mixed,
Obtain mixture；
Then 100g mixture is put into 1000ml distilled water and is impregnated, change mild fire after intense fire boiling, gauze mistake after 30min
Filter, filter residue warm water 500ml change mild fire after intense fire boiling, filter after 30min, merging filtrate, 60 DEG C of Rotary Evaporators are concentrated into
100ml obtains the crude drug that concentration is 1g/ml；
It takes albolene 2.7g, glycerin monostearate 9.6g, stearic acid 33g and atoleine 12mL to be heated to 70 DEG C to melt
Turn to oily phase；Crude drug 50mL, triethanolamine 1.1mL and ethylparaben 0.3g are separately dissolved as water phase, then slowly by water phase
It is added in oily phase, is forward stirred when being added, 5min is removed from water-bath, transdermal agent borneol 1.5g is added, and in room temperature
Under continue stirring in yellow semisolid, after condensation both ointment.
The present invention has the advantage that compared with prior art
The present invention provides one kind using anti-inflammatory and antalgic as the rules for the treatment of, forms prescription using clinically used 6 taste Chinese herbal medicine, can have
Anti-inflammation detumescence is imitated, inhibition of pain is quick, does not generate drug resistance, no allergic reaction, non-stimulated and toxic side effect can be effective
Treat the common inflammatory swelling as caused by various disease in pet diagnosis and treatment.
Specific embodiment
In the following, illustrating the present invention for embodiment, still, the present invention is not limited to following embodiments.
All raw and auxiliary materials, reagent and the instrument selected in the present invention, equipment are all well known in the art selection, but unlimited
Implementation of the invention is made, other some reagents well known in the art and equipment are applied both to the reality of following implementation of the present invention
It applies.
Have to verify ointment proposed by the invention to the pet as caused by various disease common in pet diagnosis and treatment
Inflammatory swelling generates anti-inflammatory and antalgic and inhibits the therapeutic effect of granuloma hyperplasia, is tested as follows.
1.1 material
1.1.1 animal
Weight 18-22g male mice in kunming；150 ± 50g of weight albino guinea-pig, weight 200 ± 20g rat, cleaning
Grade, half male and half female；
1.1.2 drug
Experiment Chinese medicine component by weight are as follows:
27 parts of Chinese gall
21 parts of asarum
24 parts of cortex dictamni
12 parts of olibanum
14.5 parts of myrrh
1.5 parts of borneol.
1.2 method
1.2.1 key agents and medical fluid preparation
(1) prepared by Chinese traditional medicine water extract: by compound Chinese medicine (Chinese gall 27g, asarum 21g, the cortex dictamni 24g, olibanum of 100g
12g, myrrh 16g) it is put into the distilled water of 1000mL and impregnates 2h, change mild fire, filtered through gauze after 30min after intense fire boiling；Filter residue
Warm water 500mL changes mild fire after intense fire boiling, filters after 30min, merging filtrate, and 60 DEG C of Rotary Evaporators are concentrated into 100mL,
Obtain the crude drug liquid that concentration is 1g/mL.
(2) preparation of compound Chinese Gall ointment: take albolene 2.7g, glycerin monostearate 9.6g, stearic acid 33g and
Atoleine 12mL is heated to 70 DEG C and melts as oily phase；It is separately that medical fluid 50mL, triethanolamine 1.1mL and ethylparaben 0.3g is molten
Solution is water phase, and then water phase is added slowly in oily phase, is forward stirred when being added, and 5min is removed from water-bath, is added
Borneol 1.5g, and continue stirring at room temperature in yellow semisolid, ointment is both obtained after condensation, raw drug content is in gained ointment
0.33g/g。
(3) specific as follows using the ointment of above method preparation various dose (by the dosage for adjusting crude drug liquid):
Compound Chinese Gall ointment high dose group (being 0.33g/g containing raw drug content)；
Compound Chinese Gall ointment middle dose group (being 0.16g/g containing raw drug content)；
Compound Chinese Gall ointment low dose group (being 0.08g/g containing raw drug content)；
Compound Chinese Gall ointment A group raw drug content is 3.3g/g, 10 times of clinical concentrations；
Compound Chinese Gall ointment B group raw drug content is 1.65g/g, 5 times of clinical concentrations.
(4) preparation of sensitization phase 2,4-dinitrochlorobenzene (DNCB): accurately weighing in the bottle of DNCB 100mg to l0mL, uses
70% ethyl alcohol is dissolved to l0mL, concentration 1%, is protected from light and is sealed under room temperature；
(5) prepared by stimulating phase DNCB: it is accurately weighed in the bottle of DNCB 10mg to l0mL on the day of use, it is molten with 70% ethyl alcohol
Solution is to l0mL, concentration 0.1%, is protected from light and is sealed under room temperature.
1.2.2 Animal adaptability is raised
It from receiving experimental animal, is fed according to the standard of breeding experimental animals, is seen daily during raising
Examine whether normal the rat state of mind, diet, excreta etc. or other abnormal symptoms and sign.
1.2.3 the anti-inflammatory effect experiment of compound Chinese Gall ointment
1.2.3.1 mouse ear swelling caused by dimethylbenzene is tested
Healthy mice 50, it is randomly divided into 5 groups, every group 10.That is model control group, erythromycin ointment group, five times of compound
Sub- basic, normal, high (0.08, the 0.16,0.33g/g) dosage group of ointment.The previous day of test uses 7% vulcanized sodium (Na2S) to small
Mouse the nape of the neck is lost hair or feathers, and is stopped about 1min after smearing, is cleaned up skin, the area of depilation is about 1.5cm2.Model control group
Physiological saline is given, medicine group gives various dose ointment, twice daily, continuous use one week.Ensure drug for a long time and medication
Position keeps good contact.When applying ointment, is wrapped with gauze, fixed with preservative film and medical air-permeable adhesive tape.Last
After secondary administration 1h, 0.2mL dimethylbenzene (PX) is taken to be applied to every mouse right ear, is control with left ear.After 1h, with beating for diameter 6mm
Hole machine punches the auricle of left and right auricle corresponding position, and is weighed with precision balance.Ear swelling value and ear swelling rate
It is calculated as follows.
Swelling angle value: auris dextra piece-left auricle
Ear swelling inhibiting rate: (auris dextra piece-left auricle)/left auricle × 100%
1.2.3.2 the influence experiment to mouse granuloma induced by implantation of cotton pellets
Healthy mice 50, it is randomly divided into 5 groups, every group 10.That is model control group, positive ointment group, compound Chinese Gall
Ointment low, middle and high dose groups.Chloral hydrate anesthesia, left side groin are implanted into high pressure sterilization rayon balls (10mg).Postoperative next day
After administration, ibid method makes ointment come into full contact with skin, daily to replace, and continues 7d.Model control group smears physiological saline, positive
Ointment group smears compound dexamethasone acetate emulsifiable paste, and medication group smears compound Chinese Gall ointment respectively, and 2 times/d, continuous 7d is administered.
1h washes away the de- cervical approach of drug with clear water and puts to death mouse after the last administration.Left side cotton balls is taken out, is put into 60 DEG C of baking ovens and dries for 24 hours,
Claim dry weight.
Granulomatous weight=cotton balls dry weight-cotton balls weight
Mouse granulation swelling inhibiting rate (%)=(model group granuloma average quality-administration group granuloma average quality)/
Model group granuloma average quality × 100%
1.2.4 the experiment of the analgesic activity of compound Chinese Gall ointment
1.2.4.1 acetic acid twisting is tested
Healthy mice 50, it is randomly divided into 5 groups, every group 10.That is model control group, Votalin Ointment group, five times of compound
Sub- ointment low, middle and high dose groups.Mouse the nape of the neck depilation with administrated method with caused by dimethylbenzene xylene ear swelling similar, naive mice
Physiological saline is smeared, Votalin Ointment group smears diclofenac diethylamine emulgel, and it is soft that medication group smears compound Chinese Gall respectively
2 times/d, continuous 7d is administered in cream.Ibid method makes ointment come into full contact with skin, for the last time after the 1h of administration, every group of abdominal cavity note
Penetrate 0.5% glacial acetic acid of 0.2mL (Ac) solution.Observe the writhing number and the appearance of first time writhing that each group mouse occurs in 15min
Time, record and calculate each group drug to the analgesia rate of mouse.
Writhing judgement: there is abdomen twitch in mouse, and opisthotonos, raised root of the tail portion is primary complete writhing response.
Mouse analgesia rate (%)=[(blank group writhing number-experimental group writhing number)/blank group writhing number] ×
100%
1.2.5 the safety experiment of compound Chinese Gall ointment
1.2.5.1 skin sensibiligen is tested
Intact skin group: rat 30, half male and half female is divided into blank group, compound Chinese Gall ointment A group and compound Chinese Gall
Ointment B group.Rat back hair first is shaved off with pet shave for 24 hours before administration, then uses 7%Na2S is applied to guinea pig back, stops
After about 1min, Na is washed away2S, back depilation area is about 6cm × 6cm.To guarantee that drug and medicine-feeding part keep long-term good
Contact is wrapped up with gauze, is suitably fixed using preservative film and medical air-permeable adhesive tape when applying ointment.Every 6h coating
Once, the patch being adhered to rat is removed afterwards for 24 hours to observe and respectively at 1,24,48,72h to 7d to the constitution of animal
Whether amount, the state of mind, coat fall off, skin whether exception, secretion, the character of excreta, the variation of mucous membrane and breathing, in
The animals systemic toxicity profiles performances such as pivot nervous system (twitch, shake, trembling, salivating), movable form (slack, drowsiness, stupor)
It is recorded with death condition.
Damaged skin group: taking rat 30, half male and half female, is divided into blank group, compound Chinese Gall ointment A group and five times of compound
Sub- ointment B group.Back depilating method is same as above, using the tincture of iodine to hair removal section skin degerming, using the scalpel after sterilizing in skin
On mark " # " notched cut (length of " # " word stroke about 3cm, depth are 1-3mm or so), do not damaged subcutaneously with slight oozing of blood
Connective tissue is degree, remaining operation is the same as intact skin group.
1.2.5.2 skin allergy test
Cavy 32, half male and half female is divided into basic, normal, high compound Chinese Gall ointment dosage group and positive controls.Before administration
Back is lost hair or feathers for 24 hours, and method is same as above, and the growing state for observing back coat irregularly loses hair or feathers.In order to guarantee drug and medication portion
Position comes into full contact with, and hair removal section uniformly smears each group traditional Chinese herb medicine on the left of cavy, is fixed with gauze, preservative film and medical adhesive tape soft
Cream, method are same as above.Drug is set to act on 6h in continuous skin, cavy destroys mutually fixture in order to prevent, by every globefish after administration
Mouse is single only to be fed.7d and 14d are repeated once by ibid method.
Sensitization contact: positive controls are coated with 1%DNFB, after coming into full contact with 6h, wash off drug, 7d and 14d according to
The same methods are repeated once, and observe its skin and general reaction.
Sensitization: the 14d after last coating is excited, compound Chinese Gall ointment group is uniformly applied to right side hair removal section, effect
Drug is washed away after 6h, the response situation of the animal skin allergy of observation and 0,24,48,72h, positive controls are coated with immediately
0.1%DNCB excites sensitization, and observation method is same as above.As shown in table 1, it scores, calculates the reaction mean scores of each group.Simultaneously
Notice observing whether animal has twitch, be short of breath, roll about, coughing, slobbering, asthma, the seriousness allergy such as vomiting or death.
As shown in table 2, sensitization is inferred by sensitization incidence.
1 cutaneous anaphylaxis standards of grading of table
| Cutaneous anaphylaxis situation | Score value |
| Erythema, no erythema | 0 |
| Slight erythema, it is visible reluctantly | 1 |
| Moderate erythema, hence it is evident that visible | 2 |
| Severe erythema | 3 |
| Aubergine erythema simultaneously forms eschar | 4 |
| Oedema, no oedema | 0 |
| Mild edema, it is visible reluctantly | 1 |
| Intermediate edema, hence it is evident that visible (edge is higher by surrounding skin) | 2 |
| Severe edema, cutaneous protuberance lmm, is clearly outlined | 3 |
| Severe edema, cutaneous protuberance 1mm or more simultaneously expand or have bubble or ulceration | 4 |
| Highest point total value | 8 |
Table 1
2 hypersensitive evaluation criterion of table
| Sensitization incidence (%) | Classification | Hypersensitive evaluation |
| 0-10 | Ⅰ | Without sensitization |
| 11-30 | Ⅱ | The quick property of slight will |
| 31-60 | Ⅲ | Moderate sensitization |
| 61-80 | Ⅳ | Severe sensitization |
| 81-100 | Ⅴ | Extreme sensitization |
Table 2
Note: sensitization rate (%)=number of animals/number of animals of erythema, oedema or systemic anaphylaxis reaction occur
1.2.5.3 skin irritation test
Intact skin group takes rat 20, and half male and half female is divided into compound Chinese Gall ointment A group and compound Chinese Gall ointment B
Group.It ibid loses hair or feathers, test is compareed using self left and right sides, and hair removal section is coated with traditional Chinese herb medicine on the left of animal, with preservative film, gauze
It is suitably fixed with medical air-permeable adhesive tape again after covering, use cleaning skin afterwards for 24 hours, then have through 1,24,48,72h observation recipient site
There is not erythema or oedema reaction.
Damaged skin group takes rat 20, and half male and half female is divided into compound Chinese Gall ointment A group and compound Chinese Gall ointment B
Group.It ibid loses hair or feathers, using the tincture of iodine to hair removal section skin degerming, is marked on left dorsal skin using the scalpel after sterilizing
" # " notched cut (length of " # " word stroke about 3cm, depth are 1-3mm or so) remaining operation is the same as intact skin group.According to big
Mouse erythema and oedema situation obtain the irritating average product score value of each group rat skin, evaluate each group rat skin stimulus intensity.
As shown in table 1, it scores, calculates the mean scores of every group of reaction of animals.As shown in table 2, to rat skin irritation intensity
It is evaluated.
2 results
The analysis of experimental results of the anti-inflammatory effect of 2.2 compound Chinese Gall ointment
2.2.1 the analysis of experimental results of ear swelling caused by paraxylene
As a result as follows: as shown in table 3, compared with model control group, compound Chinese Gall ointment low, middle and high dose groups and red
Mycin ointment group daily ingestion amount and weight differences are not significant (P > 0.05).As shown in table 4, compared with model control group, compound five
The ear swelling and ear swelling rate significant difference (P < 0.05) of gall nut ointment low, middle and high dose groups and erythromycin ointment group.With
Erythromycin ointment group is compared, not significant (the P > of the swelling and ear swelling rate difference of the high, medium and low dosage group of compound Chinese Gall ointment
0.05).It is compared between compound Chinese Gall ointment low, middle and high dose groups, swelling and not significant (the P > of ear swelling rate difference
0.05)。
Ear swelling caused by 3 dimethylbenzene of table tests the influence to mouse daily ingestion amount and weight
| Group | Number of animals/only | Daily ingestion amount (g) | Weight (g) |
| Model control group | 10 | 31.39±3.81 | 26.45±3.89 |
| Compound Chinese Gall ointment low dose group | 10 | 27.30±3.62 | 27.00±5.00 |
| Compound Chinese Gall ointment middle dose group | 10 | 29.07±4.53 | 27.43±4.37 |
| Compound Chinese Gall ointment high dose group | 10 | 26.04±3.73 | 26.29±4.17 |
| Erythromycin group | 10 | 26.79±4.80 | 26.28±4.01 |
Table 3
Note: compared with model control group, the equal P > 0.05. of each group
The influence of ear swelling caused by 4 compound Chinese Gall ointment paraxylene of table
| Group | Number of animals/only | Swelling (mg) | Swelling rate (%) |
| Model control group | 10 | 11.53±1.48a | 1.74±0.26c |
| Compound Chinese Gall ointment low dose group | 10 | 7.43±1.33b | 1.11±0.27b |
| Compound Chinese Gall ointment middle dose group | 10 | 6.85±1.52b | 0.98±0.22ab |
| Compound Chinese Gall ointment high dose group | 10 | 6.30±1.49b | 0.85±0.18a |
| Erythromycin ointment group | 10 | 6.51±1.97b | 0.88±0.26ab |
Table 4
Note: in table, institute's note word parent phase is not more significant (P > 0.05) than identical expression statistical result difference in data,
The female different expression statistical result significant difference (P < 0.05) of institute's note word in data.
2.2.3 to the analysis of experimental results of mouse granuloma induced by implantation of cotton pellets
As a result as follows: such as table 5 and shown, compared with model control group, compound Chinese Gall ointment low, middle and high dose groups and
Positive ointment group mouse daily ingestion amount and weight differences are not significant (P > 0.05).As shown in table 6, multiple compared with model control group
The granuloma weight differential of square Chinese gall ointment low, middle and high dose groups and positive ointment group is significant (P < 0.05).It is soft with the positive
Cream group is compared, and the granuloma weight differential of compound Chinese Gall ointment high dose group is unobvious (P > 0.05), compound Chinese Gall ointment
Low, middle dose group granuloma weight differential is significantly (P < 0.05).It is compared between compound Chinese Gall ointment low, middle and high dose groups,
Swelling difference is not significant (P > 0.05).
5 granuloma induced by implantation of cotton pellets of table tests the influence to mouse daily ingestion amount and weight
| Group | Number of animals/only | Daily ingestion amount (g) | Weight (g) |
| Model control group | 10 | 42.93±5.08 | 29.71±1.58 |
| Compound Chinese Gall ointment low dose group | 10 | 42.47±4.71 | 29.66±2.12 |
| Compound Chinese Gall ointment middle dose group | 10 | 40.07±3.46 | 30.10±2.00 |
| Compound Chinese Gall ointment high dose group | 10 | 38.91±5.00 | 29.53±2.49 |
| Positive ointment group | 10 | 40.16±4.13 | 29.59±2.08 |
Table 5
Note: compared with model control group, the equal P > 0.05. of each group
Influence of the 6 compound Chinese Gall ointment of table to mouse granuloma induced by implantation of cotton pellets
| Group | Number of animals/only | Weight that granuloma induced by implantation of cotton pellets is swollen (mg) | Inhibiting rate (%) |
| Model control group | 10 | 27.99±3.79a | |
| Compound Chinese Gall ointment low dose group | 10 | 22.58±5.72b | 19.34 |
| Compound Chinese Gall ointment middle dose group | 10 | 19.19±1.16c | 31.44 |
| Compound Chinese Gall ointment high dose group | 1 | 15.88±1.56d | 43.28 |
| Positive ointment group | 10 | 13.60±1.17d | 51.41 |
Table 6
Note: in table, institute's note word parent phase is not more significant (P > 0.05) than identical expression statistical result difference in data,
The female different expression statistics knot of institute's note word is significant (P < 0.05) in data.
The analysis of experimental results of 2.3 compound Chinese Gall Ointment on Analgesic Effect
2.3.1 acetic acid twisting is tested
As a result as follows: as shown in table 7, compared with model control group, compound Chinese Gall ointment low, middle and high dose groups and to help
His woods ointment group daily ingestion amount and weight differences are not significant (P > 0.05).As shown in table 8, compared with model control group, compound five
The writhing number and writhing time of occurrence significant difference (P < of gall nut ointment low, middle and high dose groups and Votalin Ointment group
0.05), analgesia rate is respectively 27.46%, 35.93%, 47.46% and 67.46%.Compared with Votalin Ointment group, compound
The time difference that the writhing number and writhing of Chinese gall ointment low, middle and high dose groups occur is significant (P < 0.05).Five times of compound
Sub- ointment high and low dose group is compared, and the time difference of writhing number and writhing appearance is significant (P < 0.05).Compound Chinese Gall is soft
In cream, low dose group compare, the time difference that writhing number and writhing occur is not significant (P > 0.05).
7 acetic acid twisting of table tests the influence to mouse daily ingestion amount and weight
| Group | Number of animals/only | Daily ingestion amount (g) | Weight (g) |
| Model control group | 10 | 28.27±4.67 | 28.21±5.78 |
| Compound Chinese Gall ointment low dose group | 10 | 26.17±4.18 | 27.70±3.82 |
| Compound Chinese Gall ointment middle dose group | 10 | 28.98±4.51 | 27.01±5.11 |
| Compound Chinese Gall ointment high dose group | 10 | 24.20±1.73 | 27.54±5.56 |
| Votalin Ointment group | 10 | 25.38±4.46 | 28.15±4.96 |
Table 7
Note: compared with model control group, the equal P > 0.05. of each group
8 compound Chinese Gall ointment Dichlorodiphenyl Acetate of table causes the influence of mouse writhing reaction
Table 8
The safety experiment interpretation of result of 2.4 compound Chinese Gall ointment
2.4.1 skin sensibiligen is tested
As a result as follows, as shown in table 9, compared with intact skin blank group, the compound Chinese Gall ointment A group of intact skin and
Compound Chinese Gall ointment B group, damaged skin compound Chinese Gall ointment A group and compound Chinese Gall ointment B group, damaged skin blank group
Before daily inleting appetite, administration weight and administration after weight differences it is unobvious (P > 0.05).Appetite, the state of mind, the appearance of each group rat
Gesture behavior, secretion, excreta character are all normal.As indicated, the rat skin of intact skin group is without erythema, oedema, it is damaged
After the skin group rat topical application of drug, 3d starts to form a scab, and the part 7d crust starts shedding off, without animal dead in the wound observation phase,
Have no other abnormal behaviors.
9 compound Chinese Gall ointment skin sensibiligen of table tests the influence to rat body weight
Table 9
Note: compared with intact skin blank group, the equal P > 0.05. of each group
2.4.2 skin hypersensitivity analysis of experimental results
As a result as follows, as shown in table 10, compared with positive controls, compound Chinese Gall ointment low, middle and high dose groups are given
Weight, administration 7d weight, administration 14d weight, administration 28d weight differences are unobvious (P > 0.05) before medicine, compound Chinese Gall ointment
Low dose group, compound Chinese Gall ointment middle dose group, compound Chinese Gall ointment high dose group rat without erythema, oedema react
And there is light to moderate erythema and oedema phenomenon in systemic anaphylaxis, the rat of positive controls, right side mouse back.
As shown in table 11, according to cutaneous anaphylaxis standards of grading and strength grading, five times of compound Chinese Gall ointment low dose group, compound
Sub- ointment middle dose group, compound Chinese Gall ointment high dose group sensitization rate are 0%, and sensitization intensity is I grades of sensitization, belong to weak cause
Quick property ointment.As shown in table 12, according to allergic reaction scoring and strength grading, positive controls sensitization rate is 100%, sensitization point
Grade is V grade, belongs to pole strength sensitization.
Influence of the 10 compound Chinese Gall ointment skin allergy test of table to cavy weight
Table 10
11 compound Chinese Gall ointment of table is to guinea pig skin allergic reaction scoring and strength grading
| Allergy scoring and intensity | Erythema | Oedema | Intensity |
| Compound Chinese Gall ointment low dose group | 0 | 0 | 0 |
| Compound Chinese Gall ointment middle dose group | 0 | 0 | 0 |
| Compound Chinese Gall ointment high dose group | 0 | 0 | 0 |
Table 11
12 positive controls of table are to guinea pig skin allergic reaction scoring and strength grading
| Allergy scoring and intensity | Erythema | Oedema | Systemic anaphylaxis |
| First | 1 | 0 | Nothing |
| Second | 2 | 1 | Nothing |
| Third is only | 2 | 0 | Nothing |
| 4th | 1 | 1 | Nothing |
| 5th | 0 | 1 | Nothing |
| 6th | 2 | 0 | Nothing |
| 7th | 1 | 1 | Nothing |
| 8th | 2 | 0 | Nothing |
Table 12
2.4.3 skin irritation test interpretation of result
As a result as follows, as shown in table 13, before the daily inleting appetite of each group rat, administration after weight, administration weight without statistics
Difference (P > 0.05).As shown in table 14, according to skin wound repair standards of grading and stimulus intensity standard, intact skin compound
Chinese gall ointment A group and traditional Chinese herb medicine B group and damaged skin group compound Chinese Gall ointment A group and compound Chinese Gall ointment B group
As a result, nonirritant.Intact skin compound Chinese Gall ointment A group and compound Chinese Gall ointment B group wash away drug with warm water
Afterwards 1,24,48, in the observation period after 72h, smear position without discovery erythema and oedema reaction, compare nothing in self right side
Notable difference.The compound Chinese Gall ointment A group and compound Chinese Gall ointment B group of damaged skin are the results show that on the left of rat back
Skin lesion is formed a scab at scratch, remaining skin has no any erythema and oedema, right side skin smooth, without any skin lesion.
Influence of the 13 compound Chinese Gall ointment skin irritation test of table to rat body weight
3 analysis of experimental results
3.1 compound Chinese Gall ointment anti-inflammatory and analgesic effects
This experimental study inhibiting effect of the compound Chinese Gall ointment to mouse ear swelling, compound Chinese Gall is soft as the result is shown
Cream has effective inhibitory effect to the exudation of cell liquid caused by acute inflammation.Compared with erythromycin ointment group, compound Chinese Gall
The swelling and ear swelling rate difference of the high, medium and low dosage group of ointment be not significant, shows the antiphlogistic effects of compound Chinese Gall with medication
Concentration does not have much relations, may just have strong anti-inflammatory effect related with drug itself inflammation inhibition.With positive ointment group phase
Than the granuloma weight differential of compound Chinese Gall ointment high dose group is unobvious, and compound Chinese Gall ointment is low, middle dose group granulation
Swollen weight differential is significant.Show that the effect of the inhibition granulation hyperplasia of compound Chinese Gall has relationship with Drug level, to inhibition granulation
There are most effective concentration for the effect of hyperplasia.Body will cause body and pain reaction occur, this experiment uses ice under inflammatory effect
Acetic acid causes the mouse writhing measuring analgesic effect of compound Chinese Gall ointment.The results show that compared with model control group, it is multiple
The writhing number of square Chinese gall ointment low, middle and high dose groups significantly reduces, writhing time of occurrence is obviously postponed.Its analgesia rate point
Not Wei 27.46%, 35.93%, 47.46%, illustrate that compound Chinese Gall ointment has preferable analgesic activity.
This experimental verification compound Chinese Gall ointment has anti-inflammatory and analgesic activity, compound Chinese Gall ointment main raw material(s)
It is made of Chinese gall, asarum, cortex dictamni, olibanum, myrrh and borneol.Chinese gall has the function of hygroscopic sore, is suitable for sore
Tinea pyogenic infections, skin moisture fester have different degrees of suppression to the type of many bacteriums.The survey found that Chinese gall counterpart
Many cariogenic bacterias of chamber have inhibiting effect, reduce the generation of bacterial plaque on tooth, influence the growth and metabolism of dental caries bacterium, have to tooth
Protection is made.Asarum, pungent, warm have expelling wind and clearing away cold, through-hole analgesic, warm lung and other effects.Asarum can improve generation in branch gas
The asthma of pipe reduces the inflammatory exudation that tracheae occurs, improves the function of lung.Cortex dictamni, bitter is cold in nature, be mainly used for rubeola,
Arthralgia caused by eczema, scabies, jaundice, damp and hot, damp and hot, damp and hot poisoning, has wind-dispelling, removing toxic substances, heat-clearing, dry and wet and other effects.
Cortex dictamni methanolic extract has significant anti-inflammatory effect for the swelling of rat ear caused by dinitrofluorobenzene.Olibanum, it is pungent
Bitter, temperature, pain caused by being mainly used for pain caused by qi and blood stagnation, getting injured by a fall and ulcer pain.Studies have shown that masticinic acid can be bright
The aobvious Healing for improving experimental canker sore, while also can significantly mitigate the congestion and edema of ulcer.Myrrh, bitter is mild-natured,
There is the effect of promoting blood circulation and stopping pain, detumescence and promoting granulation and scattered blood and dissolving stasis, external application has myogenic function, is commonly incorporated into ointment, it is shared with olibanum,
The function of promoting blood circulation and stopping pain can be enhanced.Borneol is slightly cold, and external application can be relieved the pain with heat-clearing, prevent wound rotten and antipruritic effect.It can be with
Improve mechanical hyperalgesia, and can be used as the therapeutic agent of chronic ache.What this ointment side used is with analgesic, life mostly
Flesh, anti-inflammatory Chinese herbal medicine, various drugs, which reconcile, to be cooperated, and ointment is made to have significant anti-inflammatory and analgesic effect.
The safety of 3.2 compound Chinese Gall ointment
External treatment of tcm has a special advantage in treatment inflammation and pain direction, surface administration can directly be absorbed in skin and
Mucomembranous surface, and it is rapid-action, the first pass effect of liver is avoided, effect has been preferably kept.Chinese medicine is made ointment and is treated
There is long history, but many studies have shown that there are many Chinese medicines at different dosages, the identical situation of administration mode
Under, it may appear that different pharmacological actions.
The purpose of acute toxicity testing is to examine the whether toxic effect of drug to be measured, by its data of statistical analysis,
Tentatively for may generate toxic effect feature, effect relation, target organ and whether to body generate harm evaluate,
Foundation is provided for subsequent experimental design.Therefore, acute toxicity test can speculate that the power of drug toxicity, toxicity play a role
Continuous speed and duration, right solution drug whether be necessary with toxicity.
Local stimulation test is one of drug safety pilot project, and the purpose is to pass through animal multiple-contact test medicine
The drug is verified whether to the whether irritating reaction in skin, eye or injection site, it is toxic to determine whether drug contains.This experiment
Mainly the drug that may cause irritation is carried out, whether observation drug causes swollen tissue, hyperemia, denaturation and necrosis etc..
This experiment is used as developing new drug cream, and local irritation test should be carried out on using position, observes by the naked eye and tissue is cut
Piece checks irritation.
Skin anaphylactic test is a kind of toxicity test, for detect contact drug repeatedly after whether can cause the allergy of skin
Reaction.Currently, studies in China external preparation anaphylaxis mostly uses greatly guinea pig back model, pass through " cutaneous anaphylaxis scoring mark
It is quasi- " it gives and scores, inquire into the sensitization of external preparation.Calculate the sensitization levels of test medicine, i.e., non-sensitization, weak sensitization, moderate
Sensitization or Qiang Zhimin.This model test operating process is relatively easy, is easy to naked eyes and judges, but the smaller Shi Buyi of medicine irritation
It is found.
Compound Chinese Gall ointment is the inflammatory swelling clinically occurred for Medical pet, and anti-inflammatory pain-stopping effect is significant,
In order to guarantee that the safety of clinical application, this experiment have also carried out cutaneous safety experimental study to compound Chinese Gall ointment.Knot
Fruit shows compound Chinese Gall ointment to body non-toxic reaction, and the nonirritant effect of compound Chinese Gall ointment is complete by cavy
After skin contacts compound Chinese Gall ointment repeatedly, sensitization of skin will not be caused to react.It is found by drug toxicity grade scale, five
Gall nut belongs to innocuous substance, nonirritant by finding that solution is made in Chinese gall to skin, mucous membrane, Eye irritation；Also, it was found that will
Chinese gall is made ointment and carries out cutaneous safety experiment, and either intact skin or damaged skin is all anti-without acute toxicity
It answers, and nonirritant and without sensitization.Confirm that this compound Chinese Gall ointment is applied to treat non-opening in veterinary clinic
It is safely and effectively.
4 conclusions
The antiphlogistic effects of 1 compound Chinese Gall ointment are significant, suitable with erythromycin effect, it will be apparent that improve caused by test
Oedema；The antiphlogistic effects of compound Chinese Gall ointment are significant, hence it is evident that inhibit granuloma hyperplasia, reduce the swollen weight of Tissue Granulation
Amount.
2 compound Chinese Gall ointment have good analgesic effect, for the writhing time of glacial acetic acid induced mice and writhing
Number has inhibiting effect.
3 pairs of ointment carry out safety experiment, the ointment without skin sensibiligen reaction, vacuum response, will not
There is allergic reaction.
Claims (2)
1. a kind of compound Chinese Gall ointment for treating pet limbs inflammatory swelling, which is characterized in that ointment component by weight
Composition include:
27 parts of Chinese gall
21 parts of asarum
24 parts of cortex dictamni
12 parts of olibanum
16 parts of myrrh
1.5 parts of borneol.
2. a kind of preparation method of ointment described in claim 1, which is characterized in that method includes the following steps:
Step 1 weighs Chinese gall 27g, asarum 21g, cortex dictamni 24g, olibanum 12g, myrrh 16g, is then comminuted into powdery, mixes
It is even, obtain mixture；
100g mixture is put into 1000ml distilled water and impregnates by step 2, changes mild fire after intense fire boiling, gauze mistake after 30min
Filter, filter residue warm water 500ml change mild fire after intense fire boiling, filter after 30min, merging filtrate, 60 DEG C of Rotary Evaporators are concentrated into
100ml obtains the crude drug liquid that concentration is 1g/ml；
Step 3 takes albolene 2.7g, glycerin monostearate 9.6g, stearic acid 33g and atoleine 12mL to be heated to 70 DEG C
Melt as oily phase；Crude drug liquid 50mL, triethanolamine 1.1mL and ethylparaben 0.3g are separately dissolved as water phase, then by water phase
It is added slowly in oily phase, is forward stirred when being added, 5min is removed from water-bath, borneol 1.5g is added, and at room temperature
Continue stirring in yellow semisolid, ointment is both obtained after condensation.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910454496.2A CN110038056A (en) | 2019-05-29 | 2019-05-29 | A kind of compound Chinese Gall ointment and preparation method thereof for treating pet limbs inflammatory swelling |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910454496.2A CN110038056A (en) | 2019-05-29 | 2019-05-29 | A kind of compound Chinese Gall ointment and preparation method thereof for treating pet limbs inflammatory swelling |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN110038056A true CN110038056A (en) | 2019-07-23 |
Family
ID=67284078
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910454496.2A Pending CN110038056A (en) | 2019-05-29 | 2019-05-29 | A kind of compound Chinese Gall ointment and preparation method thereof for treating pet limbs inflammatory swelling |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110038056A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112274597A (en) * | 2020-12-04 | 2021-01-29 | 南华大学 | Plaster for treating open wound and preparation method thereof |
| CN115448826A (en) * | 2022-09-19 | 2022-12-09 | 无锡济煜山禾药业股份有限公司 | Separation and identification of natural borneol metabolite |
-
2019
- 2019-05-29 CN CN201910454496.2A patent/CN110038056A/en active Pending
Non-Patent Citations (2)
| Title |
|---|
| 刘嘉森: "《中药研究文献摘要 第5卷》", 31 December 1993, 中国医药科技出版社 * |
| 徐宜厚: "《徐宜厚皮肤病用药心得十讲》", 31 January 2013, 中国医药科技出版社 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112274597A (en) * | 2020-12-04 | 2021-01-29 | 南华大学 | Plaster for treating open wound and preparation method thereof |
| CN115448826A (en) * | 2022-09-19 | 2022-12-09 | 无锡济煜山禾药业股份有限公司 | Separation and identification of natural borneol metabolite |
| CN115448826B (en) * | 2022-09-19 | 2023-12-08 | 无锡济煜山禾药业股份有限公司 | Separation and identification of natural borneol metabolites |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101879283B (en) | Novel membrane spraying preparation for skin trauma and preparation method | |
| Koo | Aloe vera: Antiulcer and antidiabetic effects | |
| CN104784270B (en) | A kind of swelling and pain relieving external preparation and preparation method thereof | |
| WO2006076844A1 (en) | Traditional chinese medicament for treating injuries from falls, rheumatism and ostealgia and method for manufacture thereof | |
| DE19953829C2 (en) | Nasal spray (drops) for the treatment of fever, cold and its manufacture | |
| CN110292602A (en) | Yellow burn oil formula of a kind of compound five and preparation method thereof | |
| CN101862351A (en) | Application of active parts of gallnut in preparing anti-ulcerative colitis medicine | |
| CN110038056A (en) | A kind of compound Chinese Gall ointment and preparation method thereof for treating pet limbs inflammatory swelling | |
| CN110898186A (en) | Humulus scandens dissolved granules capable of clearing heat and removing toxicity | |
| CN102836235B (en) | Traditional Chinese medicine composition for treating scalds and burns and preparation method thereof | |
| CN100353996C (en) | Medicine composition for treating soft tissue damage and osteoarthropathy and its prepn process | |
| CN105943666B (en) | Composition for nursing sensitive skin of infant and preparation method thereof | |
| JP2599160B2 (en) | Preventive and therapeutic agent for staphylococcal disease in chickens | |
| CN105326954B (en) | A kind of Chinese medicine composition and preparation method thereof for treating blear-eye | |
| CN110787104A (en) | Compound traditional Chinese medicine toothpaste and preparation method and application thereof | |
| WO2018161890A1 (en) | Application of berberine in preparing drug for treating acute soft tissue injury | |
| DE69925158T2 (en) | COMPOSITION FOR TREATING COMBUSTION | |
| CN110075169A (en) | A kind of compound percutaneous plaster and preparation method thereof for treating chronic ache | |
| KR100301263B1 (en) | Liquid-type topical therapeutic agents for male sexual dysfunction and its process | |
| CN103735494B (en) | External antipruritic ointment for treating mosquito bite and preparation method of external antipruritic ointment | |
| CN107912462A (en) | A kind of clinical laboratory's disinfection Chinese and western medicinal composition and preparation method | |
| CN114028483B (en) | Pharmaceutical composition and preparation for treating chronic soft tissue injury | |
| CN109846861B (en) | Externally applied Mongolian medicine plaster and preparation method thereof | |
| Pharmaceutical Society of Great Britain | British pharmaceutical codex | |
| KR20120090123A (en) | Composition of film forming solution for alleviation of menstrual cramp |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20190723 |