CN110036850A - It is a kind of for making the paddy rice planting method of rice Cd standard sample matrix - Google Patents
It is a kind of for making the paddy rice planting method of rice Cd standard sample matrix Download PDFInfo
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- CN110036850A CN110036850A CN201910429266.0A CN201910429266A CN110036850A CN 110036850 A CN110036850 A CN 110036850A CN 201910429266 A CN201910429266 A CN 201910429266A CN 110036850 A CN110036850 A CN 110036850A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/20—Cereals
- A01G22/22—Rice
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/20—Liquid fertilisers
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Abstract
The present invention relates to a kind of for making the implantation methods of the rice of rice Cd standard sample matrix.The present invention accounts for the rice cultivar as rice Cd standard sample matrix using the good spoke of Indica rice kind, by the application method of special vernalization and method for culturing seedlings, special mill water culture nutrient solution, the implantation methods of the rice of a set of suitable production rice Cd standard sample matrix are invented.The present invention handles rice plant using the mill water culture nutrient solution of scientific formula, and rice plant is made to generate the absorption sensibility to cadmium element since the tiller later period, is conducive to the transhipment of pustulation period cadmium element and runs up in rice.The paddy containing horizontal gradient variation heavy metal cadmium content planted out, can be used as the raw material of heavy metal standard sample, and the preparation and Safety of Food Quality monitoring for heavy metal standard sample have a very important significance.
Description
Technical field
The present invention relates to a kind of for making the implantation methods of the rice of rice Cd standard sample matrix, belongs to rice cropping
Technical field.
Background technique
Cadmium (Cd) is toxic metals, and migration in vivo is stronger, is easily absorbed by plants, and is threatened by food chain
Human health, long-term accumulation can induce cancer in human body, and initiation sclerotin is loose, influence fertility etc., seriously endanger strong to the mankind
Health.Development with people to heavy metal cadmium Pollution Study, the heavy metal cadmium problem in rice are also increasingly taken seriously.
Heavy metal standard sample has the characteristics that uniformity, stability, accuracy and repdocutbility.Its application has spread work
The numerous areas such as industry production, commerce and trade, environmental protection and health care, in production assurance, arbitration inspection, analysis test
Technology development, meterological development etc. play an important role.Currently, cereals heavy metal biological substrate types standard sample
Preparation mainly first collect plant, subsequent addition target heavy metal element artificial in the product prepares standard sample.It is this logical
The standard sample for crossing artificial addition target heavy metal element preparation, since target heavy metal is attached to cereal-based in the form of inorganic matter
Body surface face can not be combined with matrix, have with form existing for heavy metal in natural grain it is significantly different, to subsequent standards sample
The uniformity and stability of product production easily cause influence.Therefore, it plants out and contains by bioconversion method during Rice Cropping
There is the paddy of horizontal gradient variation heavy metal cadmium content, as the raw material of subsequent heavy metal standard sample matrix, for heavy metal
The preparation of standard sample and Safety of Food Quality monitoring have a very important significance.
Summary of the invention
The present invention accounts for the rice cultivar as rice Cd standard sample matrix using the good spoke of Indica rice kind, passes through spy
The application method of different vernalization and method for culturing seedlings, special mill water culture nutrient solution, invents a set of suitable production rice Cd standard sample base
The implantation methods of the rice of body, the paddy containing horizontal gradient variation heavy metal cadmium content planted out, can be used as heavy metal mark
The raw material of quasi- sample, preparation and Safety of Food Quality monitoring for heavy metal standard sample have a very important significance.
The scheme that the present invention uses includes the following steps:
It is a kind of for making the paddy rice planting method of rice Cd standard sample matrix, this method comprises the following steps:
1) rice paddy seed is dried 20 hours under the sun, is placed 3 hours in the cool by the rice paddy seed for choosing full grains,
Then seed is put into ethanol solution and impregnates 2min, filtering rear surface uses sterile water wash, then by rice paddy seed time chlorine
Acid sodium solution impregnates 5min, is ultrasonically treated 5min, and filtering rear surface uses sterile water wash；
2) 39 DEG C of sterile waters of processed seed are impregnated into 12h, takes out filtering and place 12h, then is impregnated with 39 DEG C of sterile waters
12h shows money or valuables one carries unintentionally to seed repeatedly；Seed after showing money or valuables one carries unintentionally is placed on screen frame and is cultivated, it is opaque below culture vessel root,
The mill water culture nutrient solution of replacement in every two days；
3) select on the sunny side, the greenhouse of light transmission ventilation is as paddy water planting place；When seedling grows to 15-16cm high, transplanting to water planting
In basin, 8-10cm is spaced between rice shoot, holding water planting liquid depth is 30cm；
4) jointing stage and young every 5 days of fringe tillering stage replace a mill water culture nutrient solution, during which add evaporated water daily, and adjust
Mill water culture nutrient solution pH value；
5) 8-10 days before full heading time, every 5 days cleaning water culture basins clear up green alga, replace mill water culture nutrient solution, while adding cadmium mark
Quasi- solution makes the final concentration of the cadmium in water culture basin be maintained at 10.0-100.0 mg/L；Period adds evaporated water daily, and
Adjust mill water culture nutrient solution pH value.
The configuration method of ethanol solution described in step 1) are as follows: take dehydrated alcohol 750ml, 1L is settled to sterile water, mix
Afterwards up to required ethanol solution.
The configuration method of liquor natrii hypochloritis described in step 1) are as follows: the pure liquor natrii hypochloritis 50ml of analysis is taken, with sterile water
It is settled to 1L, up to required liquor natrii hypochloritis after mixing, which needs ready-to-use.
The configuration method of mill water culture nutrient solution described in step 2 are as follows:
Stock solution 1: ammonium nitrate 108g, sodium dihydrogen phosphate 50g, potassium sulfate 87g, after dissolving respectively, mixing is settled to 1L；
Stock solution 2: calcium chloride 284g is settled to 1L after dissolution；
Stock solution 3: epsom salt 810g is settled to 1L after dissolution；
Stock solution 4: sodium ethylene diamine tetracetate 13g, ferrous sulfate heptahydrate 9g, after dissolving respectively, mixing is settled to 1L；
5: four aqueous magnesium chloride 1.80g of stock solution, ammonium molybdate tetrahydrate 0.09g, boric acid 1.11g, white vitriol 0.05g, five water sulphur
Sour copper 0.04g after dissolving respectively, adds the mixing of 50ml sulfuric acid to be settled to 1L；
Above-mentioned 5 kinds of stock solutions dilute 1000 times respectively, isometric than mixing, obtain mill water culture nutrient solution.
The pH value of mill water culture nutrient solution is adjusted described in step 4) are as follows: between 4.3-4.5.
The configuration method of cadmium standard solution described in step 5) are as follows: weigh 22.83g caddy hydrate, constant volume after dissolution
To 1L.
The rice variety is that the good spoke of Indica rice kind accounts for.It is good that good spoke accounts for ripe phase annesl, more resistant to lodging, wide adaptability,
Growth cycle is short, is suitable for water planting.It is that Xiamen University's rice breeding group uses thousand be bred as from the fine quality rice variety selection of wound
Grain weighs the quality early rice rice varieties of 30g, is authorized by Fujian province crops variety validation board within 2003.
Beneficial effect
Compared with prior art, the invention has the benefit that
1, Germination Percentage of Rice about 3% can be improved to the processing such as disinfection, ultrasound of rice paddy seed in the present invention, the sprouting stage that shows money or valuables one carries unintentionally is raw
Mould rate is significantly reduced, well developed root system, and rice shoot is healthy and strong, and tiller increases, and pest and disease damage is reduced；
2, the specially treated in the present invention to rice paddy seed during jointing stage and young fringe tiller, can make rice enter nutrition raw
Long and reproductive growth is gone forward side by side period, guarantees the vegetative reproduction of rice, and root system development is mature, lays firm foundations for heading；?
The processing in heading period can improve heavy metal Cadmium accumulation in rice in controlled range, be conducive to absorption of the cadmium in paddy
Equilibrium, and can reduce the incidence of rice pest, while pustulation period cadmium element being promoted to transport and run up in rice；
3, the paddy planted out using the present invention, growing way is uniform, full grains, the brown rice matrix Cd constituent reference standard sample of preparation
The heavy metal cadmium of the variation containing horizontal gradient has form compared with existing cereals heavy metal biological substrate types Quality Control sample
It is distributed more reasonable remarkable advantage, is more suitable for the demand of cereal determining heavy metals, is splendid cereal heavy metal analysis Quality Control
Sample, preparation and Safety of Food Quality monitoring for heavy metal Quality Control sample have a very important significance.
4, currently on the market using the brown rice matrix Cd ingredient standard of existing brown rice standard sample matrix preparation method preparation
Every gram of price 20-50 member of sample, brown rice every gram of cost of matrix Cd constituent reference standard sample of the method for the present invention preparation are raw less than 1 yuan
It produces and the economic benefit of sale is very considerable.
Detailed description of the invention
Fig. 1 is 2 full heading time rice strain performance of embodiment, and paddy well developed root system, leaf development are good.
Fig. 2 is 2 full heading time rice strain performance of comparative example, and brown necrosis is presented in the brown stain of paddy root system, rice strain depauperation, blade tip
Spot, leaf color turn yellow.
Specific implementation method:
In order to which objects and advantages of the present invention are more clearly understood, the present invention is carried out with reference to embodiments further detailed
Illustrate, the specific embodiments described herein are merely illustrative of the present invention, is not intended to limit the present invention.
Embodiment 1
1) kind is that the good spoke of Indica rice accounts for；
2) seed for choosing full grains dries seed 20 hours under the sun, places in the cool 3 hours, then will kind
Son, which is put into ethanol solution, impregnates 2min, and filtering rear surface uses sterile water wash, then rice paddy seed is used liquor natrii hypochloritis
5min is impregnated, is ultrasonically treated five minutes, filtering rear surface uses sterile water wash；
3) 39 DEG C of sterile waters of processed seed are impregnated into 12h, takes out filtering and place 12h, then is impregnated with 39 DEG C of sterile waters
12h shows money or valuables one carries unintentionally to seed repeatedly.Seed after showing money or valuables one carries unintentionally is placed on screen frame and is cultivated, it is impermeable below the container heel of culture
Light.The mill water culture nutrient solution of replacement in every two days；
4) select on the sunny side, the greenhouse of light transmission ventilation is as paddy water planting place.When seedling grows to 15-16cm high, transplanting to water planting
In basin.8-10cm is spaced between rice shoot, holding water planting liquid depth is 30cm；
5) jointing stage and young every 5 days of fringe tillering stage replace a mill water culture nutrient solution, during which add evaporated water daily, adjust water planting
Nutrient solution pH value；
6) 8-10 days before full heading time, every 5 days cleaning water culture basins clear up green alga, replace mill water culture nutrient solution, while adding cadmium mark
Quasi- solution makes the final concentration of 100.0mg/L of cadmium in mill water culture nutrient solution.Period adds evaporated water daily, adjusts water planting nutrition
Liquid pH value is between 4.3-4.5；
7) maturity period spike of rice hangs down and in harvesting when golden yellow, and sunning is rough out, can be obtained the brown rice of heavy metal cadmium content,
It can be directly as the raw material of Cd heavy metal standard sample matrix.
The formula of the ethanol solution are as follows: dehydrated alcohol 750ml is settled to 1L with sterile water, up to required second after mixing
Alcoholic solution.
The formula of the liquor natrii hypochloritis are as follows: sodium hypochlorite (analyzing pure, effective chlorine >=10%) solution 50ml, with nothing
Bacterium water is settled to 1L, and up to required liquor natrii hypochloritis after mixing, which needs ready-to-use.
The formula of the mill water culture nutrient solution are as follows:
Stock solution 1: ammonium nitrate 108g, sodium dihydrogen phosphate 50g, potassium sulfate 87g, after dissolving respectively, mixing is settled to 1L；
Stock solution 2: calcium chloride 284g is settled to 1L after dissolution；
Stock solution 3: epsom salt 810g is settled to 1L after dissolution；
Stock solution 4: sodium ethylene diamine tetracetate 13g, ferrous sulfate heptahydrate 9g, after dissolving respectively, mixing is settled to 1L；
5: four aqueous magnesium chloride 1.80g of stock solution, ammonium molybdate tetrahydrate 0.09g, boric acid 1.11g, white vitriol 0.05g, five water sulphur
Sour copper 0.04g after dissolving respectively, adds the mixing of 50ml sulfuric acid to be settled to 1L.
Above-mentioned 5 kinds of stock solutions dilute 1000 times respectively, equal proportion mixing.
The formula of the cadmium standard solution are as follows: 22.83g caddy hydrate is settled to 1L after dissolution.
Embodiment 2
1) kind is that the good spoke of Indica rice accounts for；
2) seed for choosing full grains dries seed 20 hours under the sun, places in the cool 3 hours, then will kind
Son, which is put into ethanol solution, impregnates 2min, and filtering rear surface uses sterile water wash, then rice paddy seed is used liquor natrii hypochloritis
5min is impregnated, is ultrasonically treated five minutes, filtering rear surface uses sterile water wash；
3) 39 DEG C of sterile waters of processed seed are impregnated into 12h, takes out filtering and place 12h, then is impregnated with 39 DEG C of sterile waters
12h shows money or valuables one carries unintentionally to seed repeatedly.Seed after showing money or valuables one carries unintentionally is placed on screen frame and is cultivated, it is impermeable below the container heel of culture
Light.The mill water culture nutrient solution of replacement in every two days；
4) select on the sunny side, the greenhouse of light transmission ventilation is as paddy water planting place.When seedling grows to 15-16cm high, transplanting to water planting
In basin.8-10cm is spaced between rice shoot, holding water planting liquid depth is 30cm；
5) jointing stage and young every 5 days of fringe tillering stage replace a mill water culture nutrient solution, during which add evaporated water daily, adjust water planting
Nutrient solution pH value is between 4.3-4.5；
6) 8-10 days before full heading time, every 5 days cleaning water culture basins clear up green alga, replace mill water culture nutrient solution, while adding cadmium mark
Quasi- solution makes the final concentration of 20.0mg/L of cadmium in mill water culture nutrient solution.Period adds evaporated water daily, adjusts mill water culture nutrient solution
PH value；
7) maturity period spike of rice hangs down and in harvesting when golden yellow, and sunning is rough out, can be obtained the brown rice of heavy metal cadmium content,
It can be directly as the raw material of Cd heavy metal standard sample matrix.
The formula of the ethanol solution are as follows: dehydrated alcohol 750ml is settled to 1L with sterile water, up to required second after mixing
Alcoholic solution.
The formula of the liquor natrii hypochloritis are as follows: sodium hypochlorite (analyzing pure, effective chlorine >=10%) solution 50ml, with nothing
Bacterium water is settled to 1L, and up to required liquor natrii hypochloritis after mixing, which needs ready-to-use.
The formula of the mill water culture nutrient solution are as follows:
Stock solution 1: ammonium nitrate 108g, sodium dihydrogen phosphate 50g, potassium sulfate 87g, after dissolving respectively, mixing is settled to 1L；
Stock solution 2: calcium chloride 284g is settled to 1L after dissolution；
Stock solution 3: epsom salt 810g is settled to 1L after dissolution；
Stock solution 4: sodium ethylene diamine tetracetate 13g, ferrous sulfate heptahydrate 9g, after dissolving respectively, mixing is settled to 1L；
5: four aqueous magnesium chloride 1.80g of stock solution, ammonium molybdate tetrahydrate 0.09g, boric acid 1.11g, white vitriol 0.05g, five water sulphur
Sour copper 0.04g after dissolving respectively, adds the mixing of 50ml sulfuric acid to be settled to 1L.
Above-mentioned 5 kinds of stock solutions dilute 1000 times respectively, equal proportion mixing.
The formula of the cadmium standard solution are as follows: 22.83g caddy hydrate is settled to 1L after dissolution.
Embodiment 3
1) kind is that the good spoke of Indica rice accounts for；
2) seed for choosing full grains dries seed 20 hours under the sun, places in the cool 3 hours, then will kind
Son, which is put into ethanol solution, impregnates 2min, and filtering rear surface uses sterile water wash, then rice paddy seed is used liquor natrii hypochloritis
5min is impregnated, is ultrasonically treated five minutes, filtering rear surface uses sterile water wash；
3) 39 DEG C of sterile waters of processed seed are impregnated into 12h, takes out filtering and place 12h, then is impregnated with 39 DEG C of sterile waters
12h shows money or valuables one carries unintentionally to seed repeatedly.Seed after showing money or valuables one carries unintentionally is placed on screen frame and is cultivated, it is impermeable below the container heel of culture
Light.The mill water culture nutrient solution of replacement in every two days；
4) select on the sunny side, the greenhouse of light transmission ventilation is as paddy water planting place.When seedling grows to 15-16cm high, transplanting to water planting
In basin.8-10cm is spaced between rice shoot, holding water planting liquid depth is 30cm；
5) jointing stage and young every 5 days of fringe tillering stage replace a mill water culture nutrient solution, during which add evaporated water daily, adjust water planting
Nutrient solution pH value is between 4.3-4.5；
6) 8-10 days before full heading time, every 5 days cleaning water culture basins clear up green alga, replace mill water culture nutrient solution, while adding cadmium mark
Quasi- solution makes the final concentration of 10.0mg/L of cadmium in mill water culture nutrient solution.Period adds evaporated water daily, adjusts mill water culture nutrient solution
PH value；
7) maturity period spike of rice hangs down and in harvesting when golden yellow, and sunning is rough out, can be obtained the brown rice of heavy metal cadmium content,
It can be directly as the raw material of Cd heavy metal standard sample matrix.
The formula of the ethanol solution are as follows: dehydrated alcohol 750ml is settled to 1L with sterile water, up to required second after mixing
Alcoholic solution.
The formula of the liquor natrii hypochloritis are as follows: sodium hypochlorite (analyzing pure, effective chlorine >=10%) solution 50ml, with nothing
Bacterium water is settled to 1L, and up to required liquor natrii hypochloritis after mixing, which needs ready-to-use.
The formula of the mill water culture nutrient solution are as follows:
Stock solution 1: ammonium nitrate 108g, sodium dihydrogen phosphate 50g, potassium sulfate 87g, after dissolving respectively, mixing is settled to 1L；
Stock solution 2: calcium chloride 284g is settled to 1L after dissolution；
Stock solution 3: epsom salt 810g is settled to 1L after dissolution；
Stock solution 4: sodium ethylene diamine tetracetate 13g, ferrous sulfate heptahydrate 9g, after dissolving respectively, mixing is settled to 1L；
5: four aqueous magnesium chloride 1.80g of stock solution, ammonium molybdate tetrahydrate 0.09g, boric acid 1.11g, white vitriol 0.05g, five water sulphur
Sour copper 0.04g after dissolving respectively, adds the mixing of 50ml sulfuric acid to be settled to 1L.
Above-mentioned 5 kinds of stock solutions dilute 1000 times respectively, equal proportion mixing.
The formula of the cadmium standard solution are as follows: 22.83g caddy hydrate is settled to 1L after dissolution.
The present invention is handled by disinfection, the ultrasound etc. to rice paddy seed, is improved Germination Percentage of Rice, is reduced the sprouting stage that shows money or valuables one carries unintentionally
Mildew rate；By the specially treated during jointing stage and young fringe tiller, guaranteeing the vegetative reproduction of rice, root system development is mature,
Firm foundations are laid for heading；By the processing in heading period, the product of heavy metal cadmium in rice is improved in controlled range
It is tired, be conducive to absorption equilibrium of the cadmium in paddy, reduce the incidence of rice pest, while pustulation period cadmium element can be promoted
It transports and runs up in rice；The paddy growing way of harvest is uniform, full grains, and shell resulting brown rice, includes horizontal gradient change
The heavy metal cadmium of change, and be evenly distributed, stable content, compared with existing cereals heavy metal biological substrate types Quality Control sample,
Have the advantages that significant, is splendid heavy metal quality-control sample raw material.Preparation and food quality for heavy metal Quality Control sample
Safety monitoring has a very important significance.Currently on the market using the rough of existing brown rice standard sample matrix preparation method preparation
Every gram of price 20-50 member of meter Ji Ti Cd constituent reference standard sample, the brown rice matrix Cd constituent reference standard sample of the method for the present invention preparation are every
Less than 1 yuan, the economic benefit of production and selling is very considerable for gram cost.
Comparative example 1
1) kind is that the good spoke of Indica rice accounts for；
2) seed is dried 20 hours under the sun, is placed 3 hours in the cool by the seed for choosing full grains；
3) 39 DEG C of sterile waters of processed seed are impregnated into 12h, takes out filtering and place 12h, then is impregnated with 39 DEG C of sterile waters
12h shows money or valuables one carries unintentionally to seed repeatedly.Seed after showing money or valuables one carries unintentionally is placed on screen frame and is cultivated, it is impermeable below the container heel of culture
Light.The mill water culture nutrient solution of replacement in every two days adjusts mill water culture nutrient solution pH value between 4.3-4.5.
The formula of the mill water culture nutrient solution are as follows:
Stock solution 1: ammonium nitrate 108g, sodium dihydrogen phosphate 50g, potassium sulfate 87g, after dissolving respectively, mixing is settled to 1L；
Stock solution 2: calcium chloride 284g is settled to 1L after dissolution；
Stock solution 3: epsom salt 810g is settled to 1L after dissolution；
Stock solution 4: sodium ethylene diamine tetracetate 13g, ferrous sulfate heptahydrate 9g, after dissolving respectively, mixing is settled to 1L；
5: four aqueous magnesium chloride 1.80g of stock solution, ammonium molybdate tetrahydrate 0.09g, boric acid 1.11g, white vitriol 0.05g, five water sulphur
Sour copper 0.04g after dissolving respectively, adds the mixing of 50ml sulfuric acid to be settled to 1L.
Above-mentioned 5 kinds of stock solutions dilute 1000 times respectively, equal proportion mixing.
Comparative example 2
1) kind is that the good spoke of Indica rice accounts for；
2) seed for choosing full grains dries seed 20 hours under the sun, places in the cool 3 hours, then will kind
Son, which is put into ethanol solution, impregnates 2min, and filtering rear surface uses sterile water wash, then rice paddy seed is used liquor natrii hypochloritis
5min is impregnated, is ultrasonically treated five minutes, filtering rear surface uses sterile water wash；
3) 39 DEG C of sterile waters of processed seed are impregnated into 12h, takes out filtering and place 12h, then is impregnated with 39 DEG C of sterile waters
12h shows money or valuables one carries unintentionally to seed repeatedly.Seed after showing money or valuables one carries unintentionally is placed on screen frame and is cultivated, it is impermeable below the container heel of culture
Light.The mill water culture nutrient solution of replacement in every two days；
4) select on the sunny side, the greenhouse of light transmission ventilation is as paddy water planting place.When seedling grows to 15-16cm high, transplanting to water planting
In basin.8-10cm is spaced between rice shoot, holding water planting liquid depth is 30cm；
5) jointing stage and young every 5 days of fringe tillering stage replace a mill water culture nutrient solution, while adding cadmium standard solution, make water planting nutrition
The final concentration of 20.0mg/L of cadmium in liquid.Period adds evaporated water daily, adjust mill water culture nutrient solution pH value to 4.3-4.5 it
Between；
6) 8-10 days before full heading time, every 5 days cleaning water culture basins clear up green alga, replace mill water culture nutrient solution, while addition contains
The standard solution of 20.0mg/L cadmium.Period adds evaporated water daily, adjusts mill water culture nutrient solution pH value between 4.3-4.5.
The formula of the ethanol solution are as follows: dehydrated alcohol 750ml is settled to 1L with sterile water, up to required second after mixing
Alcoholic solution.
The formula of the liquor natrii hypochloritis are as follows: sodium hypochlorite (analyzing pure, effective chlorine >=10%) solution 50ml, with nothing
Bacterium water is settled to 1L, and up to required liquor natrii hypochloritis after mixing, which needs ready-to-use.
The formula of the mill water culture nutrient solution are as follows:
Stock solution 1: ammonium nitrate 108g, sodium dihydrogen phosphate 50g, potassium sulfate 87g, after dissolving respectively, mixing is settled to 1L；
Stock solution 2: calcium chloride 284g is settled to 1L after dissolution；
Stock solution 3: epsom salt 810g is settled to 1L after dissolution；
Stock solution 4: sodium ethylene diamine tetracetate 13g, ferrous sulfate heptahydrate 9g, after dissolving respectively, mixing is settled to 1L；
5: four aqueous magnesium chloride 1.80g of stock solution, ammonium molybdate tetrahydrate 0.09g, boric acid 1.11g, white vitriol 0.05g, five water sulphur
Sour copper 0.04g after dissolving respectively, adds the mixing of 50ml sulfuric acid to be settled to 1L.
Above-mentioned 5 kinds of stock solutions dilute 1000 times respectively, equal proportion mixing.
The formula of the cadmium standard solution are as follows: 22.83g caddy hydrate is settled to 1L after dissolution.
Comparative example 3
1) kind is that the good spoke of Indica rice accounts for；
2) seed for choosing full grains dries seed 20 hours under the sun, places in the cool 3 hours, then will kind
Son, which is put into ethanol solution, impregnates 2min, and filtering rear surface uses sterile water wash, then rice paddy seed is used liquor natrii hypochloritis
5min is impregnated, is ultrasonically treated five minutes, filtering rear surface uses sterile water wash；
3) 39 DEG C of sterile waters of processed seed are impregnated into 12h, takes out filtering and place 12h, then is impregnated with 39 DEG C of sterile waters
12h shows money or valuables one carries unintentionally to seed repeatedly.Seed after showing money or valuables one carries unintentionally is placed on screen frame and is cultivated, it is impermeable below the container heel of culture
Light.The mill water culture nutrient solution of replacement in every two days；
4) select on the sunny side, the greenhouse of light transmission ventilation is as paddy water planting place.When seedling grows to 15-16cm high, transplanting to water planting
In basin.8-10cm is spaced between rice shoot, holding water planting liquid depth is 30cm；
5) jointing stage, the young replacement of a fringe tillering stage and full heading time every 5 days mill water culture nutrient solution, during which water is evaporated in addition daily
Amount adjusts mill water culture nutrient solution pH value between 4.3-4.5；
6) maturity period spike of rice hangs down and in harvest when golden yellow.
The formula of the ethanol solution are as follows: dehydrated alcohol 750ml is settled to 1L with sterile water, up to required second after mixing
Alcoholic solution.
The formula of the liquor natrii hypochloritis are as follows: sodium hypochlorite (analyzing pure, effective chlorine >=10%) solution 50ml, with nothing
Bacterium water is settled to 1L, and up to required liquor natrii hypochloritis after mixing, which needs ready-to-use.
The formula of the mill water culture nutrient solution are as follows:
Stock solution 1: ammonium nitrate 108g, sodium dihydrogen phosphate 50g, potassium sulfate 87g, after dissolving respectively, mixing is settled to 1L；
Stock solution 2: calcium chloride 284g is settled to 1L after dissolution；
Stock solution 3: epsom salt 810g is settled to 1L after dissolution；
Stock solution 4: sodium ethylene diamine tetracetate 13g, ferrous sulfate heptahydrate 9g, after dissolving respectively, mixing is settled to 1L；
5: four aqueous magnesium chloride 1.80g of stock solution, ammonium molybdate tetrahydrate 0.09g, boric acid 1.11g, white vitriol 0.05g, five water sulphur
Sour copper 0.04g after dissolving respectively, adds the mixing of 50ml sulfuric acid to be settled to 1L.
Above-mentioned 5 kinds of stock solutions dilute 1000 times respectively, equal proportion mixing.
The germination percentage and the rate that mildews of 1 embodiment of table and comparative example show
As shown in table 1, the paddy seeds sterilize by the method for the invention, being ultrasonically treated are compared to 1 paddy rice planting method of comparative example
Not only germination percentage is significantly improved, and the sprouting stage that the shows money or valuables one carries unintentionally rate of mildewing is significantly reduced.As shown in Figure 1 and Figure 2, in jointing stage and young fringe point
The comparative example 2 of the standard solution containing cadmium is added during tiller compared to the embodiment 2 in heading period addition standard solution containing cadmium, is occurred
Phenomena such as brown necrotic plaque is presented in the brown stain of paddy root system, blade tip, leaf color turns yellow, 2 full heading time paddy well developed root system of embodiment, blade
It physically well develops.
The performance of the brown rice Cd content of 2 embodiment of table and comparative example
As shown in table 2, the standard solution of heavy metal cadmium is added into mill water culture nutrient solution in full heading time using the method for the present invention
Embodiment 1, embodiment 2, embodiment 3 compared with the ratio 3 for the standard solution for being not added with heavy metal cadmium, remain basically stable in the period
In the case where, by the standard solution of the pitch-based sphere change of gradient cadmium during Rice Cropping, it can plant out and contain horizontal gradient
Change the paddy of cadmium content.
The above is only a preferred embodiment of the present invention, to those of ordinary skill in the art, it is not taking off
Under the premise of from the principle of the invention, it can also make several improvements and retouch, these improvements and modifications also should be regarded as of the invention
Protection scope.
Claims (7)
1. a kind of for making the paddy rice planting method of rice Cd standard sample matrix, which is characterized in that this method includes as follows
Step:
1) rice paddy seed is dried 20 hours under the sun, is placed 3 hours in the cool by the rice paddy seed for choosing full grains,
Then seed is put into ethanol solution and impregnates 2min, filtering rear surface uses sterile water wash, then by rice paddy seed time chlorine
Acid sodium solution impregnates 5min, is ultrasonically treated 5min, and filtering rear surface uses sterile water wash；
2) 39 DEG C of sterile waters of processed seed are impregnated into 12h, takes out filtering and place 12h, then is impregnated with 39 DEG C of sterile waters
12h shows money or valuables one carries unintentionally to seed repeatedly；Seed after showing money or valuables one carries unintentionally is placed on screen frame and is cultivated, it is opaque below culture vessel root,
The mill water culture nutrient solution of replacement in every two days；
3) select on the sunny side, the greenhouse of light transmission ventilation is as paddy water planting place；When seedling grows to 15-16cm high, transplanting to water planting
In basin, 8-10cm is spaced between rice shoot, holding water planting liquid depth is 30cm；
4) jointing stage and young every 5 days of fringe tillering stage replace a mill water culture nutrient solution, during which add evaporated water daily, and adjust
Mill water culture nutrient solution pH value；
5) 8-10 days before full heading time, every 5 days cleaning water culture basins clear up green alga, replace mill water culture nutrient solution, while adding cadmium mark
Quasi- solution makes the final concentration of the cadmium in water culture basin be maintained at 10.0-100.0 mg/L；Period adds evaporated water daily, and
Adjust mill water culture nutrient solution pH value.
2. implantation methods according to claim 1, which is characterized in that the configuration method of ethanol solution described in step 1) are as follows:
Dehydrated alcohol 750ml is taken, 1L is settled to sterile water, up to required ethanol solution after mixing.
3. implantation methods according to claim 1, which is characterized in that the configuration method of liquor natrii hypochloritis described in step 1)
Are as follows: the pure liquor natrii hypochloritis 50ml of analysis is taken, 1L is settled to sterile water, up to required liquor natrii hypochloritis after mixing, this is molten
Liquid needs ready-to-use.
4. implantation methods according to claim 1, which is characterized in that the configuration method of mill water culture nutrient solution described in step 2
Are as follows:
Stock solution 1: ammonium nitrate 108g, sodium dihydrogen phosphate 50g, potassium sulfate 87g, after dissolving respectively, mixing is settled to 1L；
Stock solution 2: calcium chloride 284g is settled to 1L after dissolution；
Stock solution 3: epsom salt 810g is settled to 1L after dissolution；
Stock solution 4: sodium ethylene diamine tetracetate 13g, ferrous sulfate heptahydrate 9g, after dissolving respectively, mixing is settled to 1L；
5: four aqueous magnesium chloride 1.80g of stock solution, ammonium molybdate tetrahydrate 0.09g, boric acid 1.11g, white vitriol 0.05g, five water sulphur
Sour copper 0.04g after dissolving respectively, adds the mixing of 50ml sulfuric acid to be settled to 1L；
Above-mentioned 5 kinds of stock solutions dilute 1000 times respectively, isometric than mixing, obtain mill water culture nutrient solution.
5. implantation methods according to claim 1, which is characterized in that adjust the pH value of mill water culture nutrient solution described in step 4)
Are as follows: between 4.3-4.5.
6. implantation methods according to claim 1, which is characterized in that the configuration method of cadmium standard solution described in step 5)
Are as follows: 22.83g caddy hydrate is weighed, is settled to 1L after dissolution.
7. implantation methods according to claim 1, which is characterized in that the rice variety is the good spoke of Indica rice kind
It accounts for.
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