CN110031959B - High-speed regulation and control device for polarization of exciting light - Google Patents
High-speed regulation and control device for polarization of exciting light Download PDFInfo
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Abstract
The invention discloses a high-speed regulation and control device for polarization of exciting light, which comprises: and the excitation light polarization regulating and controlling mechanism is used for modulating the polarization of the two or three excitation lights generated by the excitation light modulation mechanism into linearly polarized light with the polarization direction perpendicular to the plane of the optical axis of the excitation light, and transmitting the two or three modulated excitation lights to the microscopic imaging system to finish the stripe-shaped illumination required by the structured light illumination super-resolution imaging. The excitation light polarization high-speed regulation and control device can realize microsecond-order high-speed regulation and control, does not limit the clear aperture of the excitation light, and realizes high-speed modulation of the laser polarization state required by the structured light illumination super-resolution imaging technology.
Description
Technical Field
The invention belongs to the technical field of laser polarization control, and particularly relates to a high-speed regulation and control device for polarization of exciting light.
Background
The super-resolution fluorescence microscopic imaging technology can break through the limitation of optical diffraction limit and effectively observe the tiny structure of a sample, so that great attention and wide application are obtained in recent years. The structured light Illumination super-resolution microscopic imaging technology (SIM technology for short) is one of super-resolution fluorescence microscopic imaging. The SIM technology utilizes periodically distributed stripe-shaped exciting light to illuminate a sample, and in the fluorescent signal excitation process, the illuminating stripe and the spatial frequency of the sample are subjected to frequency mixing, so that high-frequency information in the sample is modulated to a low-frequency area, the high-frequency information can be detected by an optical system, and a clear image breaking through the diffraction limit is reconstructed by utilizing a fluorescence image with diffraction limit. The imaging process of the SIM technology is simultaneously operated and completed in the whole two-dimensional plane/three-dimensional space, so that the imaging speed of the SIM technology is higher compared with other super-resolution imaging technologies, and the SIM technology is suitable for researching highly dynamic samples.
In the SIM technology, improving the contrast of the light and the dark of the stripe excitation light is the key to realize high signal-to-noise ratio detection and high quality super-resolution reconstruction. The fringe-shaped excitation light is usually generated by the interference of two beams (two-dimensional SIM) or three beams (three-dimensional SIM imaging) of linearly polarized laser light which are arranged in a line, and the best interference effect can be obtained only when the polarization direction of the linearly polarized light beam is vertical to the plane of the optical axis of the light beam, so that the best fringe light and shade contrast is obtained. In addition, due to the need to reconstruct images, the SIM technique requires illuminating the sample with striped excitation light in less than or equal to three (linear SIM) or more than or equal to five (non-linear SIM) different directions, which correspond to different excitation beam arrangement directions and different optimal polarization directions. Therefore, in order to obtain an optimum imaging effect and a faster imaging speed, it is necessary to adjust the arrangement direction and polarization direction of the excitation light at a high speed. The SIM technology usually uses a spatial light modulator to change the arrangement direction of exciting light at present, and the response speed is in the sub-millisecond order; the response time of a commonly used 'liquid crystal polarization modulator' for adjusting the polarization state of the exciting light is 10ms magnitude, and the research of the SIM technology on a highly dynamic process is severely limited by the polarization modulation speed. In addition, the light-passing aperture of the existing high-speed adjustable phase delay device is small, and the central light beam and the two outer light beams cannot pass through simultaneously, so that the requirement of the SIM technology cannot be met.
The invention aims to solve the problem of polarization high-speed regulation and control of three beams/two beams of large-caliber exciting light in the SIM technology, provides a method for regulating and controlling the polarization state of multi-beam laser meeting the requirements of all SIM technologies, and can realize microsecond-order high-speed response without limiting the clear aperture.
Disclosure of Invention
Aiming at the defects in the prior art, the invention provides a high-speed regulation and control device for polarization of exciting light.
The purpose of the invention is realized by the following technical scheme:
an excitation light polarization high-speed regulation device, comprising:
and the excitation light polarization regulating and controlling mechanism is used for modulating the polarization of the two or three excitation lights generated by the excitation light modulation mechanism into linearly polarized light with the polarization direction perpendicular to the plane of the optical axis of the excitation light, and transmitting the two or three modulated excitation lights to the microscopic imaging system to finish the stripe illumination required by the structured light illumination super-resolution imaging.
Further, the excitation light modulation mechanism comprises an excitation light generation assembly, a first beam expanding lens group, a first polarization beam splitter, an achromatic half wave plate, a spatial light modulator and a first lens,
the first beam expanding lens group is used for expanding the initial exciting light generated by the exciting light generating assembly;
the first polarization beam splitter is used for splitting the expanded initial exciting light and sequentially sending the split exciting light to the achromatic half-wave plate and the spatial light modulator;
the spatial light modulator is used for modulating the exciting light received from the achromatic half-wave plate, dividing the modulated exciting light into two or three beams, and then sequentially sending the two or three beams of exciting light to the achromatic half-wave plate and the first polarization beam splitter;
the first lens is used for converging two or three beams of exciting light which sequentially pass through the achromatic half-wave plate and the first polarization beam splitter into parallel light, and then sending the two or three beams of parallel exciting light to the exciting light polarization regulating mechanism.
Further, the excitation light polarization control mechanism includes:
the polarization rotation combined wave plate is used for regulating and controlling the polarization states of the two excitation lights modulated by the excitation light modulation mechanism;
and the light shielding plate is used for removing the stray exciting light generated by the exciting light modulation mechanism.
Furthermore, the polarization rotation combined wave plate is formed by splicing 1-3 half wave plates or not less than 5 half wave plates.
Further, the excitation light polarization control mechanism further includes:
the first knife edge reflector is arranged between the excitation light modulation mechanism and the polarization rotation combined wave plate and is used for cutting out a beam of excitation light generated by the excitation light modulation mechanism in a reflection mode;
the reflecting mirror group comprises a first reflecting mirror and a second reflecting mirror, and is used for reflecting one beam of excitation light reflected and cut by the first knife-edge reflecting mirror to a position between the light shielding plate and the microscopic imaging system;
the polarization state regulating and controlling unit is used for regulating and controlling the polarization state of the exciting light reflected by the reflector group;
and the second blade reflector is used for reflecting the exciting light regulated and controlled by the polarization state regulating and controlling unit to be parallel to the exciting light modulated by the exciting light modulating mechanism and entering the microscopic imaging system together.
Further, a second lens is arranged between the first blade reflector and the first reflector.
Furthermore, a second polarization beam splitter is arranged on the excitation light modulation mechanism, the excitation light polarization regulation mechanism also comprises a third reflector, a polarization state regulation unit and a third blade reflector,
the second polarization beam splitter is used for splitting the initial excitation light generated by the excitation light modulation mechanism and sending a beam of excitation light obtained after splitting to the third reflector;
the third reflector is used for reflecting one beam of excitation light split by the second polarization beam splitter to a position between the light shielding plate and the microscopic imaging system;
the polarization state regulating and controlling unit is used for regulating and controlling the polarization state of the exciting light reflected by the third reflector;
and the third blade reflector is used for reflecting the exciting light regulated and controlled by the polarization state regulating and controlling unit to be parallel to the exciting light modulated by the exciting light modulating mechanism and entering the microscopic imaging system together.
Further, the polarization state regulating unit comprises a phase retarder and an achromatic quarter-wave plate.
Furthermore, a second beam expanding lens group and a third lens are sequentially arranged between the achromatic quarter-wave plate and the second blade reflector or between the achromatic quarter-wave plate and the third blade reflector.
Furthermore, a layer of metal reflecting film or reflection increasing dielectric film is plated on the surfaces of the first blade reflecting mirror and the second blade reflecting mirror or the third blade reflecting mirror.
The invention provides a high-speed control device for excitation light polarization, which is characterized in that an excitation light polarization control mechanism is used for modulating two or three excitation lights generated by an excitation light modulation mechanism into linearly polarized light with the polarization direction perpendicular to the plane of the optical axis of the excitation light, and the modulated two or three excitation lights are sent to a micro-imaging system to finish the stripe illumination required by imaging. According to the invention, two or three beams of excitation light are adjusted into linearly polarized light with the polarization direction perpendicular to the plane where the optical axis of the excitation light is located, so that the best light and shade contrast can be obtained through interference, and high-quality SIM imaging is obtained; in addition, the polarization direction of the exciting light is perpendicular to the plane where the optical axes of the two or three beams of exciting light are located when the arrangement direction of the exciting light is changed, so that the polarization change response speed of the exciting light is microsecond magnitude, and the clear aperture is not limited; in a word, the excitation light polarization high-speed regulation and control device can realize microsecond-order high-speed regulation and control, and cannot limit the clear aperture of the excitation light, so that the high-speed modulation of the laser polarization state required by the structured light illumination super-resolution imaging technology is realized.
Drawings
Fig. 1 is a light path diagram of a SIM system of an excitation light polarization high-speed modulation apparatus according to an exemplary embodiment 1 of the present invention;
fig. 2 is a light path diagram of a SIM system of an excitation light polarization high-speed control device according to exemplary embodiment 2 of the present invention;
FIG. 3 is a light path diagram of a SIM system of an excitation light polarization high-speed control device according to exemplary embodiment 3 of the present invention;
FIG. 4 is a block diagram of a polarization rotating combination waveplate used in the linear structured light illumination super-resolution imaging technique according to an exemplary embodiment of the present invention;
FIG. 5 is a block diagram of a polarization rotating combination waveplate used in the nonlinear structured light illumination super-resolution imaging technique in accordance with an exemplary embodiment of the present invention;
fig. 6 is a three-dimensional structural view of a first/second/third blade mirror of an exemplary embodiment of the present invention.
In the figure: 1-excitation light modulation mechanism, 101-first beam expanding lens group, 102-first polarization beam splitter, 103-achromatic half-wave plate, 104-spatial light modulator, 105-first lens, 106-laser, 107-acousto-optic tunable filter, 108-fourth mirror, 109-achromatic half-wave plate;
2-excitation light polarization regulation and control mechanism, 201-polarization rotation combined wave plate, 202-light shielding plate, 203-first blade reflector, 204-second blade reflector, 205-first reflector, 206-second reflector, 207-second lens, 208-phase retarder, 209-achromatic quarter wave plate, 2010-second beam expansion lens group, 2011-third lens, 2012-second polarization beam splitter, 2013-third reflector, 2014-third blade reflector;
3-microscopic imaging system.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
As shown in fig. 1, an excitation light polarization high-speed regulation and control device includes an excitation light modulation mechanism 1 and an excitation light polarization regulation and control mechanism 2; the excitation light polarization regulating mechanism 2 is used for modulating the polarization of the three beams of excitation light generated by the excitation light modulation mechanism 1 into linearly polarized light with the polarization direction perpendicular to the plane of the optical axis of the excitation light, and sending the three beams of modulated excitation light to the microscopic imaging system 3 to finish the stripe-shaped illumination required by the structured light illumination super-resolution imaging.
As a preferred embodiment, the excitation light modulation mechanism 1 is configured to generate initial excitation light and divide the generated initial excitation light into two or three beams, and includes an excitation light generation assembly, a first beam expansion lens group 101, a first polarization beam splitter 102, an achromatic half wave plate 103, a spatial light modulator 104, and a first lens 105, where the first beam expansion lens group 101 is configured to expand the initial excitation light generated by the excitation light generation assembly; the first polarization beam splitter 102 is configured to split the initial excitation light after beam expansion, and sequentially send the split excitation light to the achromatic half-wave plate 103 and the spatial light modulator 104; the spatial light modulator 104 is configured to modulate the excitation light received from the achromatic half-wave plate 103, divide the modulated excitation light into three beams, and sequentially transmit the three beams of excitation light to the achromatic half-wave plate 103 and the first polarization beam splitter 102; the first lens 105 is configured to converge the three excitation lights that sequentially pass through the achromatic half-wave plate 103 and the first polarization beam splitter 102 into parallel lights, and then send the parallel three excitation lights to the excitation light polarization control mechanism 2.
As a preferred embodiment, the excitation light polarization regulating mechanism 2 includes a polarization rotation combined wave plate 201, a light shielding plate 202, a first blade reflector 203, a reflector set, a polarization state regulating unit, and a second blade reflector 204, where the first blade reflector 203 is disposed between the excitation light modulation mechanism 1 and the polarization rotation combined wave plate 201, and is used to cut out a beam of excitation light generated by the excitation light modulation mechanism 1; the polarization rotation combined wave plate 201 is used for regulating and controlling the polarization states of the other two excitation lights modulated by the excitation light modulation mechanism 1; the light shielding plate 202 is used for removing stray laser generated by the excitation light modulation mechanism 1; the reflector group comprises a first reflector 205 and a second reflector 206, and is used for reflecting a beam of excitation light reflected and cut by the first knife edge reflector 203 twice, so that the beam of excitation light is reflected to a position between the light shielding plate 202 and the microscopic imaging system 3; the polarization state regulating and controlling unit is used for regulating and controlling the exciting light reflected by the reflector group to carry out polarization state regulation and control; the second blade reflector 204 is configured to reflect the excitation light modulated by the polarization state modulation unit to be parallel to the rest of the excitation light modulated by the excitation light modulation mechanism, and enable the three beams of excitation light to enter the microscopic imaging system 3 together.
Further, a second lens 207 is disposed between the first blade mirror 203 and the first mirror 205.
Further, the polarization state regulating unit includes a phase retarder 208 and an achromatic quarter waveplate 209.
A second beam expanding lens group 2010 and a third lens 2011 are sequentially arranged between the achromatic quarter wave plate 209 and the second blade reflector 204.
Further, as shown in fig. 6, the surfaces of the first blade mirror 203 and the second blade mirror 204 are plated with a metal reflective film or a reflective dielectric film.
Further, as shown in fig. 4, the polarization rotation combination wave plate 201 is formed by splicing 3 half wave plates.
The excitation light generating assembly includes a laser 106, an acousto-optic tunable filter 107, and a fourth mirror 108.
The specific light path construction method in this embodiment is as follows: laser emitted by a laser 106 passes through an acousto-optic tunable filter 107, a first beam expanding lens group 101, a first polarization beam splitter 102 and an achromatic half wave plate 103, then is modulated and reflected by a spatial light modulator 104, one beam of excitation light is changed into three beams, and the three beams of excitation light pass through the achromatic half wave plate 103 and the first polarization beam splitter 102 again and are converged by a first lens 105; two beams of excitation light on the outer side of the three beams of converged excitation light respectively pass through two opposite regions of the polarization rotation combination half-wave plate 201, the excitation light is modulated into linearly polarized light with the polarization direction perpendicular to the optical axis planes of the two beams, one beam of the center of the three beams of laser light is cut out by the first blade reflector 203, and is converged into parallel light with a small aperture by the second lens 207, the polarization state of the beam of excitation light is adjusted to be optimal by the phase retarder 208 and the achromatic quarter-wave plate 209, and then the beam of excitation light is expanded by the second beam expanding lens group 2010 and is coupled with the two beams of laser on the outer side through the second blade reflector 204 for output; and finally, emitting the three beams of laser through a lens and an objective lens on the microscopic imaging system 3, converging the three beams of laser at a sample, and illuminating the sample by using a fringe-shaped distributed light field formed after the three beams of laser are interfered.
The embodiment is suitable for polarization high-speed regulation and control of three-dimensional linear SIMs using three beams of excitation beams, the arrangement directions of the excitation beams are three directions at intervals of 60 degrees, and two beams of laser illumination samples can be realized by blocking laser passing through the phase delayer 208, so that the embodiment is suitable for two-dimensional linear SIMs of three directions at intervals of 60 degrees, wherein the arrangement directions of the excitation beams are three directions.
Example 2
As shown in fig. 2, the excitation light polarization high-speed regulation and control device includes an excitation light modulation mechanism 1 and an excitation light polarization regulation and control mechanism 2; the excitation light polarization regulating mechanism 2 is used for modulating the polarization of the two excitation lights generated by the excitation light modulation mechanism 1 into linearly polarized light with the polarization direction perpendicular to the plane of the optical axis of the excitation light, and sending the two modulated excitation lights to the microscopic imaging system 3 to finish the stripe illumination required by the structured light illumination super-resolution imaging.
As a preferred embodiment, the excitation light modulation mechanism 1 is configured to generate initial excitation light and divide the generated initial excitation light into two or three beams, and includes an excitation light generation assembly, a first beam expansion lens group 101, a first polarization beam splitter 102, an achromatic half wave plate 103, a spatial light modulator 104, and a first lens 105, where the first beam expansion lens group 101 is configured to expand the initial excitation light generated by the excitation light generation assembly; the first polarization beam splitter 102 is configured to split the initial excitation light after beam expansion, and sequentially send the split excitation light to the achromatic half-wave plate 103 and the spatial light modulator 104; the spatial light modulator 104 is configured to modulate the excitation light received from the achromatic half-wave plate 103, divide the modulated excitation light into two parts, and sequentially send two excitation lights to the achromatic half-wave plate 103 and the first polarization beam splitter 102; the first lens 105 is configured to converge the two excitation lights that sequentially pass through the achromatic half-wave plate 103 and the first polarization beam splitter 102 into parallel lights, and then send the two parallel excitation lights to the excitation light polarization control mechanism 2.
As a preferred embodiment, the excitation light polarization regulating mechanism 2 includes a polarization rotation combined wave plate 201 and a light shielding plate 202, and the polarization rotation combined wave plate 201 is used for regulating and controlling the polarization states of the two beams of excitation light modulated by the excitation light modulation mechanism 1; the mask 202 is used to remove stray laser light generated by the excitation light modulation mechanism 1.
Further, as shown in fig. 4, the polarization rotation combination wave plate 201 is formed by splicing 3 half wave plates.
The excitation light generating assembly includes a laser 106, an acousto-optic tunable filter 107, and a fourth mirror 108.
The specific light path construction method in this embodiment is as follows: excitation light emitted by a laser 106 passes through an acousto-optic tunable filter 107, a fourth reflector 108, a first beam expanding lens group 101, a first polarization beam splitter 102 and an achromatic half wave plate 103, is modulated and reflected by a spatial light modulator 104, and one excitation light is changed into two beams which pass through the achromatic half wave plate 103 and the first polarization beam splitter 102 again and are converged by a first lens 105; the two converged excitation lights respectively pass through two opposite areas of the polarization rotation combination half-wave plate 201, and the excitation lights are modulated into linearly polarized light with the polarization direction perpendicular to the optical axis planes of the two light beams; and then the light is emitted through the light shielding plate 202, the lens on the microscopic imaging system 3 and the objective lens, and the two beams of light form a fringe-shaped distribution light field after interference to illuminate the sample.
The polarization high-speed control method is suitable for polarization high-speed control of the two-dimensional linear SIM only using two excitation light beams, and the arrangement directions of the excitation light beams are three directions with an interval of 60 degrees.
Example 3
As shown in fig. 3, the excitation light polarization high-speed regulation and control device includes an excitation light modulation mechanism 1 and an excitation light polarization regulation and control mechanism 2; the excitation light polarization regulating mechanism 2 is used for modulating the polarization of the three beams of excitation light generated by the excitation light modulation mechanism into linearly polarized light with the polarization direction perpendicular to the plane of the optical axis of the excitation light, and sending the three beams of modulated excitation light to the microscopic imaging system 3 to finish the stripe-shaped illumination required by the structured light illumination super-resolution imaging.
As a preferred embodiment, the excitation light modulation mechanism 1 is configured to generate initial excitation light and divide the generated initial excitation light into two or three beams, and includes an excitation light generation assembly, a first beam expansion lens group 101, a first polarization beam splitter 102, an achromatic half wave plate 103, a spatial light modulator 104, and a first lens 105, where the first beam expansion lens group 101 is configured to expand the initial excitation light generated by the excitation light generation assembly; the first polarization beam splitter 102 is configured to split the initial excitation light after beam expansion, and sequentially send the split excitation light to the achromatic half-wave plate 103 and the spatial light modulator 104; the spatial light modulator 104 is configured to modulate the excitation light received from the achromatic half-wave plate 103, divide the modulated excitation light into two parts, and sequentially send two excitation lights to the achromatic half-wave plate 103 and the first polarization beam splitter 102; the first lens 105 is configured to converge the two excitation lights that sequentially pass through the achromatic half-wave plate 103 and the first polarization beam splitter 102 into parallel light, and then send the two excitation lights with parallel optical axes to the excitation light polarization control mechanism 2.
As a preferred embodiment, the excitation light modulation mechanism 2 is provided with a second polarization beam splitter 2012; the excitation light polarization regulating mechanism 2 comprises a polarization rotation combined wave plate 201, a light shielding plate 202, a third reflector 2013, a polarization state regulating unit and a third blade reflector 2014; the second polarization beam splitter 2012 splits the initial excitation light generated by the excitation light modulation mechanism 1, and sends a beam of excitation light obtained by splitting to the third reflector 2013; the third reflecting mirror 2013 is configured to reflect one excitation light beam split by the second polarization beam splitter 2012, and then reflect the excitation light beam to a position between the light shielding plate 202 and the microscopic imaging system 3; the polarization state regulating and controlling unit is used for regulating and controlling the polarization state of the exciting light reflected by the third reflecting mirror 2013; the polarization rotation combined wave plate 201 is used for regulating and controlling the polarization states of the two excitation lights modulated by the excitation light modulation mechanism 1; the light shielding plate 202 is used for removing stray laser generated by the excitation light modulation mechanism 1; the third blade reflector 2014 is configured to reflect the excitation light modulated by the polarization state modulation unit to be parallel to the excitation light modulated by the excitation light modulation mechanism 1, and enable the three excitation lights to enter the microscopic imaging system 3 together.
Further, the polarization state regulating unit includes a phase retarder 208 and an achromatic quarter waveplate 209.
And a second beam expanding lens group 2010 and a third lens 2011 are sequentially arranged between the achromatic quarter wave plate 209 and the third blade reflector 2013.
The surface of the third blade reflecting mirror 2013 is plated with a metal reflecting film or a reflection increasing dielectric film.
Further, as shown in fig. 4, the polarization rotation combination wave plate 201 is formed by splicing 3 half wave plates.
The excitation light generating assembly includes a laser 106, an acousto-optic tunable filter 107, a fourth mirror 108, and an achromatic half-wave plate 109.
The specific light path construction method in this embodiment is as follows: laser emitted by a laser 106 passes through an acousto-optic adjustable filter 107 and then is divided into two paths by a second polarization beam splitter 2012, the light intensity ratio of the two paths can be adjusted by an achromatic half-wave plate 103, one path of excitation light generated by the second polarization beam splitter 2012 passes through a first beam expanding lens group 101 for beam expansion and is modulated and reflected by a spatial light modulator 104 after being subjected to first polarization beam splitter 102 and the achromatic half-wave plate 103, one beam of excitation light is changed into two beams of excitation light, the two beams of excitation light pass through the achromatic half-wave plate 103 and the first polarization beam splitter 102 again and are converged by a first lens 105, the two converged beams of excitation light respectively pass through two opposite regions of a polarization rotation combination half-wave plate 201, and the excitation light is modulated into linearly polarized light with the polarization direction perpendicular to the; the second polarization beam splitter 2012 generates another path of laser light, the polarization state of the another path of laser light is adjusted to a required state through the phase retarder 208 and the achromatic quarter wave plate 209, the another path of laser light is coupled and output with the two paths of laser light generated by the second polarization beam splitter 2012 through the third knife edge reflector 2013, finally the three paths of laser light are emitted through a lens and an objective lens of the microscopic imaging system 3 and are converged at a sample, and the three beams of laser light form a light field illumination sample in a fringe-like distribution after interference.
The applicable range of this example is the same as example 1.
Example 4
Referring to example 1, a high-speed excitation light polarization control device is provided, and the specific optical path construction of this example is the same as that of example 1, except that the polarization rotation combined half-wave plate 201 is different, and the combined half-wave plate in this example is shown in fig. 5 and is formed by splicing 5 half-wave plates.
This embodiment is suitable for an excitation light polarization high-speed control device of two-dimensional (two laser beams, which block the laser beam passing through the phase retarder 208) and three-dimensional nonlinear SIM (three laser beams) in which the arrangement direction of the excitation light beams is five directions at 36 ° intervals.
The invention provides a high-speed control device for excitation light polarization, which divides generated initial excitation light into two or three beams by an excitation light modulation mechanism, the excitation light polarization control mechanism polarizes and modulates the two or three beams of excitation light generated by the excitation light modulation mechanism into linearly polarized light with the polarization direction vertical to the plane of the optical axis of the linearly polarized light, and sends the modulated two or three beams of excitation light to a microscopic imaging system to finish the stripe illumination required by imaging. The two or three beams of excitation light are adjusted into the linearly polarized light with the polarization direction perpendicular to the plane of the optical axes of the two beams of excitation light, and the excitation light is always linearly polarized light with the polarization direction perpendicular to the plane of the optical axes of the two or three beams of excitation light when the arrangement direction of the excitation light is changed, so that the optimal interference light-dark contrast can be obtained, and high-quality SIM imaging is realized; in addition, when the arrangement direction of the exciting light is changed, the exciting light respectively passes through different regions of the polarization rotation combined wave plate or the polarization state is regulated and controlled by the high-speed phase retarder with the megahertz response speed, and then the exciting light is respectively coupled in a beam expanding manner, so that the polarization change response time of the exciting light is microsecond magnitude, and the clear aperture is not limited; in a word, the excitation light polarization high-speed regulation and control device can realize microsecond-order high-speed regulation and control, and cannot limit the clear aperture of the excitation light, so that the high-speed modulation of the laser polarization state required by the structured light illumination super-resolution imaging technology is realized.
The present invention has been described in terms of the above embodiments, and various modifications, arrangements, and connections of the components may be made without departing from the scope of the invention.
Claims (8)
1. An excitation light polarization high-speed regulation device, comprising:
the excitation light polarization regulating and controlling mechanism is used for modulating the polarization of the two or three excitation lights generated by the excitation light modulation mechanism into linear polarization light with the polarization direction vertical to the plane of the optical axis of the excitation light, and transmitting the two or three modulated excitation lights to the microscopic imaging system to finish the stripe illumination required by the structured light illumination super-resolution imaging;
the excitation light polarization regulation mechanism comprises:
the polarization rotation combined wave plate is used for regulating and controlling the polarization states of the two excitation lights modulated by the excitation light modulation mechanism;
the light shielding plate is used for removing stray exciting light generated by the exciting light modulation mechanism;
wherein, exciting light polarization regulation and control mechanism still includes:
the first knife edge reflector is arranged between the excitation light modulation mechanism and the polarization rotation combined wave plate and is used for cutting out a beam of excitation light generated by the excitation light modulation mechanism in a reflection mode;
the reflecting mirror group comprises a first reflecting mirror and a second reflecting mirror, and is used for reflecting one beam of excitation light reflected and cut by the first knife-edge reflecting mirror to a position between the light shielding plate and the microscopic imaging system;
the polarization state regulating and controlling unit is used for regulating and controlling the polarization state of the exciting light reflected by the reflector group;
and the second blade reflector is used for reflecting the exciting light regulated and controlled by the polarization state regulating and controlling unit to be parallel to the exciting light modulated by the exciting light modulating mechanism and entering the microscopic imaging system together.
2. The excitation light polarization high-speed regulation and control device of claim 1, wherein the excitation light modulation mechanism comprises an excitation light generation assembly, a first beam expanding lens group, a first polarization beam splitter, an achromatic half-wave plate, a spatial light modulator and a first lens,
the first beam expanding lens group is used for expanding the initial exciting light generated by the exciting light generating assembly;
the first polarization beam splitter is used for splitting the expanded initial exciting light and sequentially sending the split exciting light to the achromatic half-wave plate and the spatial light modulator;
the spatial light modulator is used for modulating the exciting light received from the achromatic half-wave plate, dividing the modulated exciting light into two or three beams, and then sequentially sending the two or three beams of exciting light to the achromatic half-wave plate and the first polarization beam splitter;
the first lens is used for converging two or three beams of exciting light which sequentially pass through the achromatic half-wave plate and the first polarization beam splitter into parallel light, and then sending the two or three beams of parallel exciting light to the exciting light polarization regulating mechanism.
3. The excitation light polarization high-speed regulation and control device of claim 1, wherein the polarization rotation combined wave plate is formed by splicing 1-3 half wave plates or not less than 5 half wave plates.
4. The excitation light polarization high-speed modulation device according to claim 1, wherein a second lens is disposed between the first blade mirror and the first mirror.
5. The excitation light polarization high-speed regulation and control device of claim 1, wherein the excitation light modulation mechanism is provided with a second polarization beam splitter, the excitation light polarization regulation and control mechanism further comprises a third reflector, a polarization state regulation and control unit and a third blade reflector,
the second polarization beam splitter is used for splitting the initial excitation light generated by the excitation light modulation mechanism and sending a beam of excitation light obtained after splitting to the third reflector;
the third reflector is used for reflecting one beam of excitation light split by the second polarization beam splitter to a position between the light shielding plate and the microscopic imaging system;
the polarization state regulating and controlling unit is used for regulating and controlling the polarization state of the exciting light reflected by the third reflector;
and the third blade reflector is used for reflecting the exciting light regulated and controlled by the polarization state regulating and controlling unit to be parallel to the exciting light modulated by the exciting light modulating mechanism and entering the microscopic imaging system together.
6. The excitation light polarization high-speed regulation device according to claim 1 or 5, wherein the polarization state regulation unit comprises a phase retarder and an achromatic quarter-wave plate.
7. The excitation light polarization high-speed regulation device according to claim 6, wherein a second beam expansion lens group and a third lens are sequentially arranged between the achromatic quarter-wave plate and the second blade reflector or the third blade reflector.
8. The excitation light polarization high-speed modulation device according to claim 7, wherein a metal reflective film or a reflection increasing dielectric film is plated on the surfaces of the first blade reflector, the second blade reflector or the third blade reflector.
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Effective date of registration: 20230309 Address after: 303-1, Floor 3, Building 4, No. 9 Hospital, Life Science Park, Changping District, Beijing 102206 Patentee after: Beijing Nathan Optoelectronic Technology Co.,Ltd. Address before: 100101 Beijing city Chaoyang District Datun Road No. 15 Patentee before: Institute of Biophysics, Chinese Academy of Sciences |