CN110018558A - A kind of Portable fluorescence microscope and its working method - Google Patents
A kind of Portable fluorescence microscope and its working method Download PDFInfo
- Publication number
- CN110018558A CN110018558A CN201910440493.3A CN201910440493A CN110018558A CN 110018558 A CN110018558 A CN 110018558A CN 201910440493 A CN201910440493 A CN 201910440493A CN 110018558 A CN110018558 A CN 110018558A
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- CN
- China
- Prior art keywords
- light
- box body
- emitting window
- front shroud
- fluorescence microscope
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/0004—Microscopes specially adapted for specific applications
- G02B21/0008—Microscopes having a simple construction, e.g. portable microscopes
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/06—Means for illuminating specimens
Abstract
The present invention relates to a kind of Portable fluorescence microscope and its working method, including a box body, the box body side offers light inlet, and box body top surface offers light-emitting window, and box bottom offers the light admission port immediately below light-emitting window；The object lens being equipped with right above light admission port in box body, box body top are equipped with the eyepiece right above light-emitting window, and 45 ° of dichroic beamsplitter is equipped in the travel path of incident light of light inlet and tilted right above the object lens；It is located at light inlet on the outside of box body and LED light source, the exciter filter being equipped on the inside of light inlet in box body and the edge filter on the downside of light-emitting window is installed.Portable fluorescence microscope of the present invention has the characteristics that lightweight, small in size and easy to carry, easy to operate, easy to use, easily fabricated, it can be achieved that produce in enormous quantities, can be realized Rapid identification object in non-lab environment.
Description
Technical field
The present invention relates to a kind of Portable fluorescence microscope and its working methods.
Background technique
Equipment volume for observation sample is larger, weight weight, thus the observation of big multisample all can only in laboratory into
Row；Since such large-scale instrument is inconvenient to carry outgoing, so that Rapid identification object cannot achieve in non-lab environment.
Summary of the invention
In view of this, easy to use, small in size, light-weight is portable the object of the present invention is to provide a kind of structure is simple
Formula fluorescence microscope and its working method.
The present invention is realized using following scheme: a kind of Portable fluorescence microscope, including a box body, the box body side are opened
Equipped with light inlet, box body top surface offers light-emitting window, and box bottom offers the light admission port immediately below light-emitting window；In box body
The object lens being located at right above light admission port are installed, the eyepiece being equipped with right above light-emitting window at the top of box body, the object lens are just
Top is equipped in the travel path of incident light of light inlet and the dichroic beamsplitter of 45 ° of inclination；It is located on the outside of box body into light
LED light source is installed at mouthful, the exciter filter being equipped on the inside of light inlet in box body and on the downside of light-emitting window
Edge filter.
Further, it is provided in the box body to insert exciter filter, edge filter and dichroic beamsplitter
Slot；The mounting base to install object lens is provided in the box body, the intracorporal trailing flank of box offers the vertical chute,
The mounting base trailing flank is provided with the sliding block being slidably matched with the vertical chute.
Further, the box body includes rear bottom plate, the rectangular box on front side of rear bottom plate and covers in rectangular box
On front shroud, the front shroud U-shaped profile is simultaneously bolted on rear bottom plate；Front shroud side opens up promising
The recessing groove that LED light source is stepped down；The vertical chute is located at rear bottom plate leading flank, and the mounting base is fixed by front shroud.
Further, the rear bottom plate back side is provided with the mounting groove to insert battery.
Another technical solution of the present invention: a kind of microscopical working method of Portable fluorescence as described above, by microscope water
Laying flat keeps it face-up, opens front shroud, covers front shroud again after adjusting the position of object lens；Then it is vertical to hold microscope
Rising makes its light passing mouth down, and sample to be tested is placed on immediately below microscopical light admission port, opens LED light source, passes through eyepiece energy
Enough observe the architectural characteristic of sample to be tested.
Compared with prior art, the invention has the following advantages: Portable fluorescence microscope of the present invention have lightweight,
It is the features such as small in size and easy to carry, easy to operate, it is easy to use, it is easily fabricated, it can be achieved that mass production, makes non-laboratory
Rapid identification object can be realized in environment.
To make the objectives, technical solutions, and advantages of the present invention more comprehensible, specific embodiment and phase will be passed through below
Attached drawing is closed, invention is further described in detail.
Detailed description of the invention
Fig. 1 is isometric front view of the embodiment of the present invention；
Fig. 2 is rear isometric view of the embodiment of the present invention；
Fig. 3 is the front view that the embodiment of the present invention saves front shroud；
Fig. 4 is light path principle figure of the embodiment of the present invention；
Fig. 5 is working principle diagram of the embodiment of the present invention；
Figure label explanation: bottom plate, 150- square after 100- box body, 110- light admission port, 120- slot, 130- the vertical chute, 140-
Shape framework, 160- front shroud, 170- mounting groove, 200- object lens, 300- eyepiece, 400- dichroic beamsplitter, 500-LED light source,
600- exciter filter, 700- edge filter, 800- mounting base, 900- battery.
Specific embodiment
As shown in Fig. 1 ~ 5, a kind of Portable fluorescence microscope, including a box body 100,100 side of box body are offered
Light inlet, box body top surface offer light-emitting window, and box bottom offers the light admission port 110 immediately below light-emitting window；In box body
The object lens 200 being located at right above light admission port, the eyepiece 300 being equipped with right above light-emitting window at the top of box body 100, institute are installed
It states and 45 ° of dichroic beamsplitter 400 is equipped in the travel path of incident light of light inlet and tilted right above object lens；Box body
100 outsides, which are located at light inlet, is equipped with LED light source 500, the exciter filter 600 being equipped on the inside of light inlet in box body
And the edge filter 700 on the downside of light-emitting window；LED light source uses low-power consumption monochromatic source, light parallel radiation to excitation
Optical filter, parallel exciting light are reflected into object lens through dichroic beamsplitter, and by being irradiated to sample to be tested after object lens optically focused, sample is swashed
The irradiation that shines issues the longer transmitting light of wavelength later, and transmitting light passes through object lens, dichroic beamsplitter, finally by edge filter
Capture the light imaging for allowing the wavelength penetrated.Sample to be tested is excited after light irradiation, can issue longer than excitation wavelength
Emit light, which can intercept this and emit light to be imaged, and make to be observed composition of sample characteristic and be more clear；Relative to tradition
Large-scale instrument, Portable fluorescence microscope of the present invention have the characteristics that lightweight, small in size and easy to carry while easy to operate,
It is easy to use, it is easily fabricated, it can be achieved that mass production, can be realized Rapid identification object in non-lab environment.
In the present embodiment, it is provided in the box body 100 to insert exciter filter 600,700 and of edge filter
The slot 120 of dichroic beamsplitter 400；The mounting base 800 to install object lens 200, the box are provided in the box body 100
Intracorporal trailing flank offers the vertical chute 130, and 800 trailing flank of mounting base is provided with the cunning being slidably matched with the vertical chute
Block (not shown)；The length of the vertical chute is 12cm, and being moved up and down along the vertical chute by mounting base 800 can achieve tune
The purpose of whole object distance, to obtain optimal imaging effect.
In the present embodiment, internal optics carry out in confined space in box body when test, and box body and mounting base are equal
It is formed by environment-friendly materials polylactic acid 3D printing.Entire microscopical outer dimension is 7.5 × 6.5 × 3.5cm, and quality is about 210
g；Before the box body 100 includes rear bottom plate 140, the rectangular box 150 on front side of rear bottom plate and covers on rectangular box
Cover board 160,160 U-shaped profile of front shroud are simultaneously bolted on rear bottom plate 140；Front shroud side offers
The recessing groove stepped down for LED light source；The vertical chute 130 is located at rear 140 leading flank of bottom plate, and the mounting base 800 is by front cover
Plate is fixed, and mounting base front end heads on front shroud medial surface after covering front shroud, fixes mounting base by frictional force
Position directlys adopt front shroud and is fixed in order to which rubber strip can be arranged in mounting base front end in increasing friction force, fixed form
Simply, it can simplify microscope internal structure.
In the present embodiment, the rear bottom plate back side is provided with the mounting groove 170 to insert battery 900, entire micro-
Mirror is powered by battery 900.
A kind of microscopical working method of Portable fluorescence as described above, electronmicroscopic level, which was fallen, keeps it face-up,
Front shroud is opened, covers front shroud again after adjusting the position of object lens；Then holding microscope standing makes its light passing mouth down, will
Sample to be tested is placed on immediately below microscopical light admission port, opens LED light source, the knot of sample to be tested can be observed by eyepiece
Structure characteristic.
Any technical solution disclosed in aforementioned present invention unless otherwise stated, if it discloses numberical range,
Disclosed numberical range is preferred numberical range, and any it should be appreciated by those skilled in the art preferred numberical ranges
The only obvious or representative numerical value of technical effect in many enforceable numerical value.It, can not since numerical value is more
Exhaustion, so the present invention just discloses technical solution of the component values to illustrate the present invention, also, the above-mentioned numerical value enumerated is not
The limitation to the invention protection scope should be constituted.
If the present invention is open or relates to the components or structural member of connection fastened to each other, unless otherwise stated,
It is fixedly connected it is to be understood that connection (such as connecting using bolt or screw) can be removedly fixed, it is understood that are as follows:
It is non-removable to be fixedly connected with (such as riveting, welding), certainly, connection fastened to each other or integral structure (such as make
Manufactured with casting technique is integrally formed) replaced (obviously can not be using except integrally formed technique).
In addition, for indicating the art of positional relationship or shape applied in any technical solution disclosed in aforementioned present invention
Its meaning includes approximate with its, similar or close state or shape to language unless otherwise stated.
Either component provided by the invention by multiple individual component parts either assembled, or one
The separate part that forming technology manufactures.
Finally it should be noted that: the above embodiments are merely illustrative of the technical scheme of the present invention and are not intended to be limiting thereof；To the greatest extent
The present invention is described in detail with reference to preferred embodiments for pipe, it should be understood by those ordinary skilled in the art that: still
It can modify to a specific embodiment of the invention or some technical features can be equivalently replaced；Without departing from this hair
The spirit of bright technical solution should all cover within the scope of the technical scheme claimed by the invention.
Claims (5)
1. a kind of Portable fluorescence microscope, it is characterised in that: including a box body, the box body side offers light inlet, box
Body top surface offers light-emitting window, and box bottom offers the light admission port immediately below light-emitting window；It is equipped with to be located in box body and lead to
Object lens right above optical port, box body top are equipped with the eyepiece right above light-emitting window, are equipped with position right above the object lens
In in the travel path of incident light of light inlet and tilting 45 ° of dichroic beamsplitter；It is located at light inlet on the outside of box body and LED is installed
Light source is equipped with the exciter filter on the inside of light inlet and the edge filter on the downside of light-emitting window in box body.
2. Portable fluorescence microscope according to claim 1, it is characterised in that: be provided in the box body to insert
The slot of exciter filter, edge filter and dichroic beamsplitter；The installation to install object lens is provided in the box body
Seat, the intracorporal trailing flank of box offer the vertical chute, and the mounting base trailing flank is provided with to be slidably matched with the vertical chute
Sliding block.
3. Portable fluorescence microscope according to claim 2, it is characterised in that: the box body includes rear bottom plate, is located at
The rectangular box on front side of bottom plate and the front shroud on rectangular box is covered afterwards, the front shroud U-shaped profile simultaneously passes through spiral shell
It tethers and is connected on rear bottom plate；Front shroud side opens up the recessing groove that promising LED light source is stepped down；The vertical chute is located at rear bottom plate
Leading flank, the mounting base are fixed by front shroud.
4. Portable fluorescence microscope according to claim 3, it is characterised in that: it is described after the bottom plate back side be provided with to
Insert the mounting groove of battery.
5. a kind of microscopical working method of Portable fluorescence as claimed in claim 3, it is characterised in that: put electronmicroscopic level
Keep it face-up, opens front shroud, cover front shroud again after adjusting the position of object lens；Then holding microscope standing makes
Sample to be tested is placed on immediately below microscopical light admission port by its light passing mouth down, is opened LED light source, can be seen by eyepiece
Measure the architectural characteristic of sample to be tested.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910440493.3A CN110018558A (en) | 2019-05-24 | 2019-05-24 | A kind of Portable fluorescence microscope and its working method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910440493.3A CN110018558A (en) | 2019-05-24 | 2019-05-24 | A kind of Portable fluorescence microscope and its working method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN110018558A true CN110018558A (en) | 2019-07-16 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910440493.3A Pending CN110018558A (en) | 2019-05-24 | 2019-05-24 | A kind of Portable fluorescence microscope and its working method |
Country Status (1)
| Country | Link |
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| CN (1) | CN110018558A (en) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB201114767D0 (en) * | 2010-08-27 | 2011-10-12 | Univ Leland Stanford Junior | Microscopy imaging device with advanced imaging properties |
| CN104267488A (en) * | 2014-10-11 | 2015-01-07 | 中国科学院重庆绿色智能技术研究院 | Optical microscope beam splitter device |
| CN108107562A (en) * | 2018-02-06 | 2018-06-01 | 福州大学 | A kind of Portable fluorescence microscope and its method of work based on smart mobile phone |
| CN209803446U (en) * | 2019-05-24 | 2019-12-17 | 福州大学 | Portable fluorescence microscope |
-
2019
- 2019-05-24 CN CN201910440493.3A patent/CN110018558A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB201114767D0 (en) * | 2010-08-27 | 2011-10-12 | Univ Leland Stanford Junior | Microscopy imaging device with advanced imaging properties |
| CN104267488A (en) * | 2014-10-11 | 2015-01-07 | 中国科学院重庆绿色智能技术研究院 | Optical microscope beam splitter device |
| CN108107562A (en) * | 2018-02-06 | 2018-06-01 | 福州大学 | A kind of Portable fluorescence microscope and its method of work based on smart mobile phone |
| CN209803446U (en) * | 2019-05-24 | 2019-12-17 | 福州大学 | Portable fluorescence microscope |
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