CN1100145C - Bioactivity protective agent and its use - Google Patents
Bioactivity protective agent and its use Download PDFInfo
- Publication number
- CN1100145C CN1100145C CN 99123728 CN99123728A CN1100145C CN 1100145 C CN1100145 C CN 1100145C CN 99123728 CN99123728 CN 99123728 CN 99123728 A CN99123728 A CN 99123728A CN 1100145 C CN1100145 C CN 1100145C
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- China
- Prior art keywords
- bioactivator
- alive
- keeping
- protective agent
- prior
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Abstract
The present invention relates to a bioactivator and the use thereof. Gelled polysaccharide in the prior art can be only used for alive keeping of general liquid plant tissue. The present invention has the technical scheme that alginic acid and trehalose dihydrate are made into a bioactivator according to a certain proportion, and then the bioactivator is prepared into a water solution with the concentration of 0.1 to 0.3 mol/l. The bioactivator is used for alive keeping and refreshment of medicines, biological agents, frozen foods, cooling foods, garden stuff and flowers. The bioactivator has the advantages of innocuity, innocuousness, no side effect and health care function. The bioactivator realizes the preservation at normal temperature of 25 to 35 DEG C, and the storage life is prolonged to 2 to 5 times of that of the prior art. The bioactivator solves the problem of biologic cold chain on any state of bioactive products. The bioactivator has relative low cost, so that the bioactivator has a bright future.
Description
The present invention relates to biological chemical field, a kind of bioactivity protective agent and uses thereof of more specifically saying so.
At present, in plant tissue culture technique, substratum adds a kind of one-tenth glue polysaccharide, to solve plant biological keep-alive problem, makes the cell suspension productive rate reach 100%, and can preserve at normal temperatures more than 5 days.This one-tenth glue polysaccharide main component is a Lalgine, its keep-alive principle be Lalgine in the water penetrating fluid, can form a kind of compact protective film at cell surface, so that protein is not damaged.This technology can only be used to solve the keep-alive of liquid plant tissue.
The object of the invention is at weak point of the prior art; and provide a kind of keep-alive that can expand on other bio-active products to use; thoroughly solve all bio-active products on any state, realization normal temperature is preserved unlikely inactivation and was lost efficacy or putrid alive-keeping agent.
Purpose of the present invention can reach by following measure:
Bioactivity protective agent is by making with following weight percentages: Lalgine 1-5%, trehalose 95-99%; The purposes of bioactivity protective agent is the application in biotechnological formulation and medicine, biochemical product and medicine, frozen product, cooling food, the keep-alive of fruits and vegetables flower freshness.
Lalgine (C
6H
8O
6).
Trehalose C
12H
22O
112H
2O
The present invention has following advantage than prior art: 1. alive-keeping agent is realized 25-35 ℃ of preservation of normal temperature, and nontoxic, harmless, nothing is paid effect, has nourishing function, and preservation period prolongs 2-5 doubly than prior art; 2. it is fresh-keeping to realize that all bio-active products reach high-effective living-protective on any state; 3. relative cost is low.Its purposes is to solve biological cold chain problem, is used for aspects such as biotechnological formulation, biochemical drug, frozen product, cooling food, fruits and vegetables and flower freshness keep-alive, is mixed with the aqueous solution of 0.1-0.3 mol during use.
Be described further with regard to two embodiment below:
Embodiment 1:
Lalgine 1 gram and trehalose 99 gram preparation biologically active agents 100 grams, get 73 grams then and be dissolved in 73000 milliliter water, be mixed with 0.1 mol concentration, be placed in biotechnological formulation and the medicine and use.
The active storage rate of redissolution after the bioactivity protective agent drying
Bring back to life after active storage rate (%) the temperature-time drying sample do not add the alive-keeping agent sample (℃) (D)
CHOD?PVL?RAT CHOD?PVL?RAT35 90 93 100?100 15 5.0?7.050 50 93 98 92 10 6.0?8.070 30 96 99 95 15 6.0?7.0
Annotate: the 1.CHOD---rCO
2.PVL----Live Poliomyelitis Vaccine
3.RAT----Rabies Antiserum
As can be seen from the above table: after having used bioactivity protective agent, the rCO of sample is brought back to life than big 7 times without bioactivity protective agent in dry back, and Live Poliomyelitis Vaccine is big 20 times, and Rabies Antiserum is big 12 times.
Embodiment 2:
Lalgine 15 grams and trehalose 285 grams are made into biologically active agent, get 219 gram bioactivity protective agents and add 73000 milliliter water, be mixed with the concentration of 0.3 mol, be placed on the keep-alive of biochemical product aspect:
Keep-alive condition after the bioactivity protective agent freeze-drying
Do not add alive-keeping agent and add royal jelly＜-5, alive-keeping agent Bacillus subtilus＜-10 ℃＞25 ℃ ℃＞30 ℃ (animal) bioactive peptide＜-5 ℃＞30 ℃
Claims (2)
1, a kind of bioactivity protective agent is characterized in that it is by making with following weight percentages:
Lalgine 1-5% trehalose 95-99%
2, alive-keeping agent as claimed in claim 1 is the application in biotechnological formulation and medicine, biochemical product and medicine, frozen product, cooling food, the keep-alive of fruits and vegetables flower freshness.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 99123728 CN1100145C (en) | 1999-11-17 | 1999-11-17 | Bioactivity protective agent and its use |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 99123728 CN1100145C (en) | 1999-11-17 | 1999-11-17 | Bioactivity protective agent and its use |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1259571A CN1259571A (en) | 2000-07-12 |
| CN1100145C true CN1100145C (en) | 2003-01-29 |
Family
ID=5282941
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 99123728 Expired - Fee Related CN1100145C (en) | 1999-11-17 | 1999-11-17 | Bioactivity protective agent and its use |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1100145C (en) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1299582C (en) * | 2005-04-30 | 2007-02-14 | 江南大学 | Method for producing frozen dough from trehalose treated yeast freeze-proof fermentation process |
| CN104542943A (en) * | 2015-01-20 | 2015-04-29 | 青岛崂好人海洋生物技术股份有限公司 | Seaweed natural fresh-keeping liquid for fruits and vegetables and preparation method of seaweed natural fresh-keeping liquid |
| CN104585308A (en) * | 2015-01-20 | 2015-05-06 | 青岛崂好人海洋生物技术股份有限公司 | Natural seaweed preservation liquid for vegetables and preparation method thereof |
| CN105165806B (en) * | 2015-07-23 | 2017-02-01 | 齐鲁工业大学 | Trehalose-containing flowering phase extender for fresh cut flowers and preparation method for trehalose-containing flowering phase extender |
-
1999
- 1999-11-17 CN CN 99123728 patent/CN1100145C/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| CN1259571A (en) | 2000-07-12 |
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| C14 | Grant of patent or utility model | ||
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| CF01 | Termination of patent right due to non-payment of annual fee |