CN102511673A - Coenzyme Q10 premix and its preparation method and use - Google Patents
Coenzyme Q10 premix and its preparation method and use Download PDFInfo
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- CN102511673A CN102511673A CN2011104108505A CN201110410850A CN102511673A CN 102511673 A CN102511673 A CN 102511673A CN 2011104108505 A CN2011104108505 A CN 2011104108505A CN 201110410850 A CN201110410850 A CN 201110410850A CN 102511673 A CN102511673 A CN 102511673A
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Abstract
The invention discloses a coenzyme Q10 premix. The coenzyme Q10 premix belongs to the field of animal feed additives. The coenzyme Q10 premix comprises: by weight, 2 to 5% of coenzyme Q10 and 95 to 98% of carriers. The invention also discloses a preparation method of the coenzyme Q10 premix. The preparation method comprises that coenzyme Q10 and the carriers are mixed uniformly according to an equivalent incremental method. The invention also discloses a use of the coenzyme Q10 premix in improvement of pork quality, a pork color, production performances and a pork water-holding capacity and in reduction of a drip loss. The coenzyme Q10 premix can effectively improve pork quality and a pork color, improve a pork red value, reduce a pork yellow value, improve a pork water-holding capacity, and reduce a drip loss. In addition, the preparation method of the coenzyme Q10 premix is simple, has a low cost and is convenient for use.
Description
Technical field
The invention belongs to the animal feed additive field, be specifically related to a kind of Co-Q10 pre-mixing agent that improves pork matter, yellowish pink, and the preparation method of this pre-mixing agent and application.
Background technology
Co-Q10 (CoQ10) is the fat-soluble quinones that extensively exists in the organism, and the number of its side chain iso-amylene unit of the ubiquinone of separate sources is different, and the ubiquinone in the mankind and the mammalian body is 10 iso-amylene units, so claim Co-Q10.Co-Q10 is that activator and immunopotentiator are breathed in cellular metabolism, have anti-oxidant, cardiac stimulant, anti-stress with the radicals scavenging function.
Co-Q10 is mainly used in the supplemental treatment in diseases such as angiocardiopathy, cancer, Parkinson's; And, in cosmetics, oral hygiene goods, health product and food additives, use also relatively extensively in the application of aspects such as sports medical science and antifatigue effect.
At present, the Co-Q10 formulation comprises: Co-Q10 sheet, Co-Q10 soft capsule, coenzyme Q 10 injection, coenzyme Q10 capsule etc.
Existing Co-Q10 formulation patent mainly contains: " the dispersible Co-Q10 dry powder of water " (publication number is CN1633245A) discloses coenzyme q-10 has been carried out the powder composition that emulsification obtains in the solution of polysaccharide gelatin substrate component, be used for adding in cosmetics, beverage, the liquid food." containing dry powder composite of Co-Q10 and preparation method thereof " (publication number is CN1969833A) discloses by 1%~60% Co-Q10, the dry powder composite of forming with excipient, embedding property wall material and anti-oxidant etc." compound coenzyme Q 10 medicine prepn " (publication number is CN101019854A) discloses by the composition of processing more than among a kind of in Co-Q10 and Troxerutin, inosine, ATP, Glycerin Fructose, coacetylase, amino acid, CDPC, the vitamin or two." a kind of coenzyme Q 10 injection " (publication number is CN101278907A) discloses and comprised the active component Co-Q10; Solubilizer polyethylene glycol 15-hydroxy stearic acid ester and solvent injection water can also add one or more the composition in cosolvent, acidity regulator, osmotic pressure regulator, the stabilizing agent." topical co-enzyme Q 10 formulations and method for using thereof " (publication number is CN102018693A) discloses the application of the tumor growth rate in the CoQ10 topical formulations reduction animal target." a kind of coenzyme Q 10 mini-pill and preparation method thereof " (publication number CN101703477A) discloses pharmaceutical carriers such as Co-Q10 and excipient, adhesives and has been prepared from.Mainly there is following problem in the disclosed technical scheme of above-mentioned patent application or document: because solubility is extremely low in the Co-Q10 water, need take in the amount that many bigger than reality so water dispersible dry powder will reach the medical function of expection.The parenteral solution formulation is used loaded down with trivial details, stress be big to animal population, be not suitable for as being used for the administration of veterinary clinic colony.Solution is all the organic solvent hydrotropy at present, and toxicity is big, residual height, and the rising of carrier cost, and medicine was separated out easily when this formulation was used for livestock and fowl drinking water simultaneously.Preparation cost such as micropill and micro emulsion is higher, veterinary clinic is promoted and had certain limitation.
At present, Co-Q10 is used for aspects such as people's health care, food, medical treatment, cosmetics, does not have to find to be used to improve the report of pork matter, yellowish pink aspect.
Summary of the invention
The object of the invention is to provide a kind of Co-Q10 pre-mixing agent, and this pre-mixing agent can be used as feed addictive and uses, can improve pig meat, improve the yellowish pink of pig.
Another purpose of the present invention is to provide the preparation method of above-mentioned Co-Q10 pre-mixing agent.
The present invention's the 3rd purpose is to provide the purposes of above-mentioned Co-Q10 pre-mixing agent.
The present invention realizes through following technical proposal:
A kind of Co-Q10 pre-mixing agent, its constituent and percentage by weight thereof are: Co-Q10 2~5%, carrier 95~98%.
Carrier described in the above-mentioned Co-Q10 pre-mixing agent is meant a kind of of zeolite powder, corn flour or starch etc.
Co-Q10 pre-mixing agent of the present invention is a pulvis.
The preparation method of above-mentioned Co-Q10 pre-mixing agent comprises the Co-Q10 drying, crosses 65 mesh sieves; With carrier drying, cross 50 mesh sieves; According to the equivalent incremental method, Co-Q10 and equivalent carrier are placed in the mixer, stirring and evenly mixing gets the Co-Q10 pre-composition; The carrier of adding and ubiquinone pre-composition equivalent in mixer stirs then; Add carrier according to the equivalent incremental method successively, stir; In mixer, add remaining carrier at last, stirring and evenly mixing promptly gets the Co-Q10 pre-mixing agent.
Described Co-Q10 and carrier all can be bought on market.
Co-Q10 is improving meat quality, is improving the purposes aspect the pork look.
The purposes of Co-Q10 aspect raising pig production performance.
The purposes of Co-Q10 aspect raising pork myoglobin content, the yellow value of reduction lay up period.
Co-Q10 is in that to improve pork be waterpower, reduce the purposes aspect the drip loss.
The purposes of Co-Q10 aspect the oxidation of inhibition pig oxidation myoglobins.
Above-mentioned Co-Q10 pre-mixing agent is improving meat quality, is improving the purposes aspect the pork look.
The purposes of above-mentioned Co-Q10 pre-mixing agent aspect raising pig production performance.
The purposes of above-mentioned Co-Q10 pre-mixing agent aspect raising pork myoglobin content, the yellow value of reduction lay up period.
Above-mentioned Co-Q10 pre-mixing agent is in that to improve pork be waterpower, reduce the purposes aspect the drip loss.
The purposes of above-mentioned Co-Q10 pre-mixing agent aspect the oxidation of inhibition pig oxidation myoglobins.
The application of Co-Q10 pre-mixing agent of the present invention mixes Co-Q10 pre-mixing agent and basal diet and gets final product, and the additive capacity of Co-Q10 in feed is the 10mg/kg feed.
Advantage that the present invention has and beneficial effect: (1) Co-Q10 pre-mixing agent of the present invention can improve pork matter effectively, improves yellowish pink.(2) Co-Q10 pre-mixing agent of the present invention can improve the red value of pork, reduce yellow value.(3) to improve pork be waterpower to Co-Q10 pre-mixing agent of the present invention, reduce drip loss.(4) Co-Q10 pre-mixing agent of the present invention can suppress the oxidation of pig oxidation myoglobins.(5) Co-Q10 pre-mixing agent preparation method of the present invention is simple, and method for using is simple, and cost is low.
The specific embodiment
Below in conjunction with specific embodiment, further set forth the present invention.These embodiment only are used to explain the present invention, and are not used in the scope of restriction requirement protection of the present invention.
The preparation of embodiment 1 Co-Q10 pre-mixing agent
Carry out according to following method:
(1) Co-Q10 (Agricultural University Of Nanjing pharmacological evaluation chamber provides) is dried to water content and is no more than 3%, cross 65 mesh sieves; Zeolite powder (Ninghai County is good to be provided with chemical industry Co., Ltd) is dried to water content and is no more than 5%, crosses 50 mesh sieves.
(2) get Co-Q10 40g, zeolite powder 1960g.According to the equivalent incremental method, earlier zeolite powder 40g and Co-Q10 40g are placed in the mixer of cleaning, stirred premix 5 minutes, form the Co-Q10 pre-composition; In mixer, add zeolite powder 80g then, mixed 5 minutes; Progressively mix according to the equivalent incremental method successively; In mixer, add at last, mix 15 minutes, promptly get Co-Q10 pre-mixing agent 2000g to mixing with remaining zeolite powder.Be encapsulated as the 1kg/ bag with clean bag.
The preparation of embodiment 2 Co-Q10 pre-mixing agents
Carry out according to following method:
(1) Co-Q10 is dried to water content and is no more than 3%, cross 65 mesh sieves, corn flour is dried to water content and is no more than 5%, crosses 50 mesh sieves.
(2) get Co-Q10 60g, zeolite powder 1940g.Earlier corn flour 60g and Co-Q10 60g are placed in the mixer of cleaning, mixed 5 minutes, form the Co-Q10 pre-composition; In mixer, add corn flour 120g then, mixed 5 minutes; Progressively mix according to the equivalent incremental method successively; Add remaining corn flour at last in mixer, mix 15 minutes, promptly obtain Co-Q10 pre-mixing agent 2000g to mixing.Be encapsulated as the 1kg/ bag with clean bag.
The preparation of embodiment 3 Co-Q10 pre-mixing agents
Carry out according to following method:
(1) Co-Q10 is dried to water content and is no more than 3%, cross 65 mesh sieves, carrier starch is dried to water content and is no more than 5%, crosses 50 mesh sieves.
(2) get Co-Q10 100g, starch 1900g.According to the equivalent incremental method, earlier starch 100g and Co-Q10 100g are placed in the mixer of cleaning, mixed 5 minutes, form the Co-Q10 pre-composition; In mixer, add starch 200g then, mixed 5 minutes; Progressively mix according to the equivalent incremental method successively; In mixer, add remaining starch at last and mix 15 minutes, promptly obtain Co-Q10 pre-mixing agent 2000g to mixing.Be encapsulated as the 1kg/ bag with clean bag.
Embodiment 4 Co-Q10 pre-mixing agents improve the contrast test of meat quality, yellowish pink
(1) experiment medicine: the Co-Q10 pre-mixing agent of the embodiment of the invention 1,2,3 preparations;
Control drug is vitamin E (the star animal husbandry of north, Nanjing).
(2) test method: song breed Co., Ltd carries out in sky, Jining, Shandong in this test.Selecting body weight for use is (65 ± 5) kg, and close, the healthy PIC of age in days is hybridized 70 of market pigs for five yuan, is divided into 5 groups at random, 14 every group.The Co-Q10 pre-mixing agent (being numbered the 1st, 2,3 group respectively) that test group is fed the embodiment of the invention 1,2,3 preparations respectively adds the amount of Co-Q10 and presses the calculating of 10mg/kg feed in the feed.Other establishes vitamin E medicine control group (addition is the 50mg/kg feed) and blank group, and it is identical that each organizes basal diet, is corn-dregs of beans type powder, raises 40 days.Measure following project:
1 growth performance: record duration of test feed total flow, weightening finish, calculating feedstuff-meat ratio.
2 meats:
(1) pH value:, be expressed as pH45min, pH24h respectively respectively at killing the pH value of measuring eye muscle chest section meat appearance behind back 45min and 0~4 ℃ of freezer storage 24h.
(2) yellowish pink:
1. surperficial yellowish pink determination method: kill back 1-2h and 0~4 ℃ of preservation 24h, 72h, the yellowish pink of 120h meat appearance, record L value, a value, b value with measuring.
2. chemical assay: measure A640, meat appearance total pigment concentration=A640 * 680 (units: μ g/g).
(3) drip loss: get 0~4 ℃ of meat appearance of storing 24h, be trimmed to the sliced meat of L * b * h=5 * 3 * 2.5cm, (W weighs
1), hook meat appearance one end with thin wire, muscle fibre is hung in the polythene film bag of inflation vertically downward; Tighten sack, avoid meat appearance to contact, be suspended in the refrigerator with the bag wall; Store 24h for 0~4 ℃, take out meat appearance, wipe (the W that weighs after the juice of meat appearance top layer gently away with filter paper
2).Drip loss (%)=[(W
1-W
2)/W
1] * 100
(4) be waterpower: get 0~4 ℃ of meat appearance of storing 24h; Weigh (W1), respectively cover one deck hospital gauze at meat appearance upper and lower, gauze respectively fills up 18 stratification qualitative analysis filter paper outward; Filter paper respectively fills up one deck hard outward and writes and use plastic base plate; To fill up good meat appearance then and be put on the platform of pressure appearance, at the uniform velocity slowly shake the crank of pressure appearance, and platform risen to demonstrate till the reading that is equivalent to 35kg on the pressure gauge dial gauge and keep this pressure 5min; Remove pressure then rapidly, take out and pressed the meat appearance and the (W that weighs immediately
2).Percentage of water loss (%)=[(W
1-W
2)/W
1] * 100
(5) the tender degree of muscle: the fat of meat appearance surface attachment is removed, bandaged in the plastic film bag of packing into, place 0~4 ℃ of refrigerator slaking 24h.Take out the meat appearance of slaking, room temperature is placed 1h.Then, open the package, thermometer is inserted the muscle center, bandage again, keep sack upwards, put into 80 ℃ of thermostat water baths and heat, reach till 70 ℃ until the muscle central temperature.Take out meat appearance, room temperature is placed and is made muscle be cooled to 20 ℃, by cutting the sliced meat that the thickness width is 1.0cm in vertical direction with muscle fibre, presses the operation of tenderometer operation instruction, 10 shear force values of each meat appearance record, calculating horizontal mean.Unit is with newton (N) or kilogram (kg) expression.
(6) moisture adopts direct drying method to measure, and intramuscular fat adopts the Suo Shi extraction process to measure.
3 myoglobins are measured
Get 0~4 ℃ and preserve 24h, 72h, the meat appearance 2g of 120h, adding 20mL concentration is the PBS buffer solution of 0.04MpH6.8,10800r/min stirs homogeneous 25s under the room temperature.Homogenizing fluid places ice bath to place 1h, in 10000g, 10~15 ℃ of following centrifugal 30min, gets supernatant and detects then.Measure myoglobins total amount (Mb), oxymyoglobin (MbO2), metmyoglobin (MetMb).
4 biochemical indicators
The eye muscle sample is preserved 24h in-20 ℃, surveys MDA (MDA), superoxide dismutase (SOD) after getting the homogenate of 100g meat appearance after thawing, and by specification is operated.
(3) result of the test:
1 growth performance (seeing table 1).1st, 2,3 groups of feedstuff-meat ratios all are lower than blank group and vitamin E group, explain with vitamin E and compare that Co-Q10 can improve the growth performance of pig.
Table 1 ubiquinone
10Comparative test result to the influence of growing and fattening pigs production performance
Group | The blank group | The vitamin E group | The 1st group | The 2nd group | The 3rd group |
Feedstuff-meat ratio | 3.15 | ?3.07 | 3.06 | 3.00 | 3.04 |
2 meats
(1) pH value (seeing table 2).1st, pH45min, pH24h, difference all not significantly (P>0.05) between 2,3 each group.
Table 2 ubiquinone
10Comparative test result to growing and fattening pigs meat Effect on Performance
Index | The blank group | The vitamin E group | The 1st group | The 2nd group | The 3rd group |
pH 45min | 6.26±0.16 | 6.41±0.16 | 6.17±0.21 | 6.28±0.25 | 6.37±0.21 |
pH 24h | 5.54±0.15 | 5.58±0.15 | 5.54±0.10 | 5.58±0.06 | 5.77±0.11 |
(2) yellowish pink (seeing table 3)
The pig eye muscle is stored 24h, and the 2nd group of L value significantly is lower than blank group (P<0.05).The red a value of each group reduces along with the prolongation of period of storage.Each measuring point, the Co-Q10 group is compared with the blank group, a value difference heteropole significantly raise (P<0.01).When 1~2h and 24h, the 2nd group of a value is significantly higher than vitamin E group (P<0.05), and this proves ubiquinone
10The effect that improves a value decrease speed, stablizes a value is arranged, explain that it can improve the muscle myoglobin content, thereby improve yellowish pink performance.Compare with the blank group, eye muscle when 1~2h and 24h, the 2nd group of b value significantly raise (P<0.05).At lay up period, the b value is respectively organized in test all has the trend that reduces gradually, and this also shows ubiquinone
10Can reduce the yellow value of lay up period muscle.
Table 3 ubiquinone
10Comparative test result to the yellowish pink influence of growing and fattening pigs
(3) drip loss, be waterpower (seeing table 4)
Compare with the blank group for the 1st group, drip loss significantly reduces (P<0.05), is that waterpower and intramuscular fat significantly raise (P<0.05), and shearing force difference is (P<0.01) extremely significantly.Compare with the blank group for the 2nd group, drip loss significantly reduces (P<0.05), and shearing force difference is (P<0.01) extremely significantly.1st, compare shearing force significant difference (P<0.05) with the vitamin E group for 2 groups.Proof is compared with vitamin E, and Co-Q10 can reduce the drip loss of muscle, and raising is a waterpower, has guaranteed histiocytic integrality, and the minimizing sarcoplasm oozes out, thereby has increased the tender degree of meat, improved meat, and the 1st, 2 group of effect is remarkable.
Table 4 Co-Q10 to the pork drip loss, be the comparative test result of waterpower
Index | The blank group | The vitamin E group | The 1st group | The 2nd group | The 3rd group |
Drip loss (%) | 2.95±0.12 a | 2.51±0.20 ab | 2.70±0.20 ab | 2.46±0.16 b | 2.41±0.08 b |
Be waterpower (%) | 36.54±1.26 a | 37.68±1.04 ab | 39.05±0.84 ab | 37.89±0.71 ab | 40.21±1.09 b |
Shearing force (kg) | 5.09±0.53 aA | 4.59±0.27 bAB | 4.22±0.31 bcB | 4.16±0.18 cB | 4.12±0.36 cB |
Intramuscular fat (%) | 3.06±0.24 a | 3.27±0.14 ab | 3.23±0.16 ab | 3.38±0.29 ab | 3.37±0.33 b |
Moisture (%) | 70.49±1.36 | 71.40±1.58 | 71.84±1.23 | 70.81±0.48 | 71.40±1.41 |
(4), yellowish pink (seeing table 5)
1st, 2,3 groups of muscle total pigment contents all are higher than blank group and vitamin E group.Explanation is compared with vitamin E, ubiquinone
10Can slow down the degraded of pigment, have and improve yellowish pink effect.
Table 5 ubiquinone
10Comparative test result to the influence of the yellowish pink cellulose content of growing and fattening pigs eye muscle (μ g/g)
Group | 24h | 72h | 120h |
The 1st group | 37.45±1.47 bcAB | 17.45±1.26 bcAB | 16.55±2.16 b |
The 2nd group | 42.26±1.04 bBC | 17.45±1.59 bcAB | 13.15±1.13 ab |
The 3rd group | 41.03±1.81 bB | 18.13±1.13 cB | 14.96±1.57 ab |
The vitamin E group | 33.09±2.27 acA | 12.92±1.71 abAB | 11.79±1.86 a |
The blank group | 29.92±2.04 aA | 11.33±1.49 aA | 11.11±0.45 a |
3, myoglobins (seeing table 6)
Compare with the blank group; The 1st group of eye muscle stored 24h, and when 72h and 120h, metmyoglobin significantly reduces (P<0.05); Oxymyoglobin significantly raise (P<0.05); When the 2nd group of eye muscle stored 24h and 72h, metmyoglobin significantly reduced (P<0.05), oxymyoglobin significantly raise (P<0.05).Compare with the vitamin E group, the 1st, 2,3 group of each index difference is not significantly (P>0.05) all, and this shows that Co-Q10 can suppress the formation of the oxidized inhibition metmyoglobin of oxidation myoglobins, thereby stable yellowish pink.
Table 6 ubiquinone
10Comparative test result to the influence of growing and fattening pigs eye muscle myoglobin content
4, biochemical indicator (seeing table 7)
Compare with the blank group for the 1st group, SOD content extremely significantly raises (P<0.01), compares SOD content significantly raise (P<0.05) with the vitamin E group.1st, compare with the blank group for 2 groups, MDA content all significantly reduces (P<0.05), explain with vitamin E and compare that Co-Q10 can significantly improve yellowish pink vividness, the tender degree of raising muscle.
Table 7 ubiquinone
10Comparative test result to growing and fattening pigs eye muscle biochemical indicator
Group | MDA(nmol/mg?prot) | SOD(U/mg?prot) |
The 1st group | 1.10±0.33 b | 15.58±1.71 bB |
The 2nd group | 1.12±0.25 b | 11.24±1.85 aAB |
The 3rd group | 1.29±0.15 ab | 11.72±0.93 abAB |
The vitamin E group | 1.25±0.31 ab | 10.31±0.44 aAB |
The blank group | 1.67±0.13 a | 7.74±0.95 aA |
Claims (10)
1. a Co-Q10 pre-mixing agent is characterized in that its constituent and percentage by weight thereof are: Co-Q10 2~5%, carrier 95~98%; Described carrier is meant zeolite powder, corn flour or starch.
2. the preparation method of the described Co-Q10 pre-mixing agent of claim 1 is characterized in that comprising with the Co-Q10 drying, crosses 65 mesh sieves; With carrier drying, cross 50 mesh sieves; According to the equivalent incremental method, Co-Q10 and equivalent carrier are placed in the mixer, stirring and evenly mixing gets the Co-Q10 pre-composition; In mixer, add and the carrier of ubiquinone pre-composition equivalent stirring and evenly mixing then; Add carrier, stirring and evenly mixing according to the equivalent incremental method successively; In mixer, add remaining carrier at last, stirring and evenly mixing promptly gets the Co-Q10 pre-mixing agent.
3. Co-Q10 is improving meat quality, is improving the purposes aspect the pork look.
4. Co-Q10 is in the purposes that improves aspect the pig production performance.
5. Co-Q10 is in that to improve pork be waterpower, reduce the purposes aspect the drip loss.
6. the described Co-Q10 pre-mixing agent of claim 1 is improving meat quality, is improving the purposes aspect the pork look.
7. the described Co-Q10 pre-mixing agent of claim 1 is in the purposes that improves aspect the pig production performance.
8. the described Co-Q10 pre-mixing agent of claim 1 is improving the pork myoglobin content, is reducing the purposes aspect the yellow value of lay up period.
9. the described Co-Q10 pre-mixing agent of claim 1 is in that to improve pork be waterpower, reduce the purposes aspect the drip loss.
10. the described Co-Q10 pre-mixing agent of claim 1 is in the purposes that suppresses aspect the oxidation of pig oxidation myoglobins.
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CN102870970A (en) * | 2012-10-24 | 2013-01-16 | 合肥市爱博生物技术有限公司 | Growth promoter for livestock and poultry |
CN105533166A (en) * | 2016-01-19 | 2016-05-04 | 安徽农业大学 | Feed additive for enhancing pig body oxidative stress resistance and improving meat quality and use method of feed additive |
CN106615683A (en) * | 2017-03-06 | 2017-05-10 | 武汉新联大生物有限公司 | Feed additive containing polyene phosphatidyl choline |
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CN105533166A (en) * | 2016-01-19 | 2016-05-04 | 安徽农业大学 | Feed additive for enhancing pig body oxidative stress resistance and improving meat quality and use method of feed additive |
CN106615683A (en) * | 2017-03-06 | 2017-05-10 | 武汉新联大生物有限公司 | Feed additive containing polyene phosphatidyl choline |
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Application publication date: 20120627 |