CN110004043A - A kind of unicellular capture micro-fluidic chip - Google Patents
A kind of unicellular capture micro-fluidic chip Download PDFInfo
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Abstract
The present invention relates to a kind of unicellular capture micro-fluidic chips, comprising: functional layer and cover plate layer；Functional layer includes: the multiple functional areas of silicon wafer ontology and modification on silicon wafer ontology；Multiple functional areas include at least: sample feeding and pretreating zone, capturing function area and liquid waste processing area；Sample feeding and pretreating zone are made of cell liquid flow inlet, import liquid storage tank, the flow channel for being provided with miniature dispersion column；Capturing function area is formed by being provided with bumper post and capturing the minisize reaction pond of trap array；Liquid waste processing area is made of the flow pass, outlet liquid storage tank and waste liquid outflux for being provided with miniature dispersion column；Cell liquid flow inlet is connected to import liquid storage tank；Import liquid storage tank is connected to by means of flow channel with minisize reaction pond；Minisize reaction pond is connected to by means of flow pass with outlet liquid storage tank；Export liquid storage tank and waste liquor stream outlet.Micro-fluidic chip provided by the invention can be realized capture in the uniform sample introduction of cell current-carrying liquid and the chip of individual cells.
Description
Technical field
The invention belongs to microfluidic art more particularly to a kind of unicellular capture micro-fluidic chips.
Background technique
Microflow control technique has been widely used in the fields such as physico-chemical analysis and biologic medical since development.Micro-fluidic chip base
Good platform is provided for the research of miniature scale in micro & nano technology, since scale is close, and analysis efficiency height, flux height etc.
Feature, the cell analysis on microchip have numerous advantages, can mention for medical analysis, drug screening, body monitoring etc.
For effective Research foundation and accurately analyze result.
In cell analysis, be directed to single celled analysis and controlling cellular morphology, be cell in vivo or ex vivo situation
Under the directions such as precise determination be of great significance.It is different from the fabric analysis of regular growth group or cell ball, micro element
Focus on research direction is to realize the positioning and analysis of individual cells, such as carries out single celled capture in cell current-carrying liquid,
In order to subsequent functional application.
In the prior art, it is studied about the micro element of cell capture extensive.
A kind of cell capture device is described in Chinese invention patent CN107338183A, which passes through setting filtering
Layer is provided with the different through-hole of inlet and outlet cross-sectional area on filter layer and realizes cell falling into automatically and be not easy to flow with flow liquid
Out, realize that one is retained in each through-hole is unicellular by size Control.But the realization of the function of the device relies on multilayer knot
Structure, therefore there are certain technological difficulties during micro-nano technology；And under larger flow conditions, since flow direction does not exist with cell
In same vertical height, therefore it is easy to happen the case where cell is carried over through-hole.
A kind of unicellular capture chip is described in Chinese invention patent CN105441307A, which passes through setting liquid stream
Layer, layers of elastomeric film and driving mechanism realize efficient unicellular capturing function, and realize stronger cellular localization function, make slender
Born of the same parents are not easy to be detached from from site.But the realization of the function of the chip is complex, driving mechanism and elastic membrane is needed to configure, in micro-nano
Biggish cost is needed in machining process.
A kind of cell capture device is described in U.S. patent Nos US20150004687A1, which passes through setting basket
Body and filter, by filter deployment in the importing and the filtering function of CTC of in basket, realizing cell dispersion liquid.But the dress
The capture for setting and being not implemented monolayer time, is filtered effect to CTC by the filter of fine through hole.
Summary of the invention
(1) technical problems to be solved
For existing technical problem, the present invention provides a kind of unicellular capture micro-fluidic chip, chip manufacture
Process flow is simple, by the way that the structures such as reasonable dispersion column, bumper post and capture trap are arranged, can be realized the equal of cell current-carrying liquid
Capture in the chip of even sample introduction and individual cells.
(2) technical solution
In order to achieve the above object, the main technical schemes that the present invention uses include:
A kind of unicellular capture micro-fluidic chip, comprising: functional layer and cover plate layer；
Functional layer includes: the multiple functional areas of silicon wafer ontology and modification on silicon wafer ontology；
Multiple functional areas include at least: sample feeding and pretreating zone, capturing function area and liquid waste processing area；
Sample feeding and pretreating zone are led to by cell liquid flow inlet, import liquid storage tank, the inflow for being provided with miniature dispersion column
Road composition；
Capturing function area is formed by being provided with bumper post and capturing the minisize reaction pond of trap array；
Liquid waste processing area is made of the flow pass, outlet liquid storage tank and waste liquid outflux for being provided with miniature dispersion column；
Cell liquid flow inlet is connected to import liquid storage tank；
Import liquid storage tank is connected to by means of flow channel with minisize reaction pond；
Minisize reaction pond is connected to by means of flow pass with outlet liquid storage tank；
Export liquid storage tank and waste liquor stream outlet；
Cover plate layer is combined with functional layer by encapsulation.
Preferably, import liquid storage tank, flow channel, minisize reaction pond, flow pass and outlet liquid storage tank are having the same
Depth H 1；
Miniature dispersion column, bumper post and capture trap array height H2 having the same；
Capture array arrangement form can be adjusted according to concrete application, realize chip flexible combination, improve application.
Preferably, import liquid storage tank and outlet liquid storage tank are round or oval.
Preferably, the miniature dispersion post shapes in flow channel and flow pass are cylindrical body or Elliptic Cylinder；
Adjacent miniature dispersion column is shifted to install along flow liquid direction.
Preferably, depth dimensions H1 and height dimension H2 meets H1 > H2, and the diameter of H1-H2 < individual cells.
Preferably, bumper post is cylindrical body, semicylinder or Elliptic Cylinder.
Preferably, it is positioned close to minisize reaction pond and the bumper post quantity of flow channel communication port side is incremented by by column；
It is positioned close to minisize reaction pond and the bumper post quantity of flow pass communication port side is successively decreased by column.
Preferably, capture trap array is made of each multiple capture traps for arranging the arrangement that is staggered；
It captures trap and is equipped with cellular retention area and carrying object guiding region.
Preferably, cellular retention area lateral depth is H3, longitudinal width H4, and size meets H3 >=individual cells diameter, H4
> individual cells diameter；
Carrying object guiding region is gap structure, and quantity N is 0~5, gap width H5；
The diameter of 2 μm of < H5 < individual cells；
At a plurality of gap, 180 ° of < of the initial 0 °≤θ of angle in two gaps of outermost.
Preferably, the material of functional layer is dimethyl silicone polymer；
The material of cover plate layer is dimethyl silicone polymer or glass.
(3) beneficial effect
The beneficial effects of the present invention are: the unicellular capture micro-fluidic chip of one kind provided by the invention has below beneficial to effect
Fruit:
(1) chip involved in the present invention only has single layer, and structure is simple, significantly reduction processing cost, and cell is in fluid
It can be stabilized under the conditions of shearing force in capture trap, be not easy to flow out, function is realized preferable.
(2) micro-fluidic chip provided in the present invention can be realized unicellular positioning capture in the chip, pass through parameter
Setting, within the scope of the cell suspending liquid entrance velocity of 0.1~0.5 μ l/s, the cell capture efficiency of this chip reach 95% with
On, reach Cell Homeostasis capture after, single sample consumption comparison the culture of comparable sodium Tissue Culture Dish be effectively reduced 90% with
On, this chip, which is applied to the fields such as drug screening, cell research, has low consumption, efficient feature.
Detailed description of the invention
Fig. 1 is the three dimensional structure diagram of the unicellular capture micro-fluidic chip of one kind provided by the invention；
Fig. 2 is the three-dimensional structure of the unicellular capture micro-fluidic chip of one kind provided by the invention along Figure 1A-A directional profile knot
Structure schematic diagram；
Fig. 3 is the structural schematic diagram of the capture trap of the unicellular capture micro-fluidic chip functional areas of one kind provided by the invention.
[description of symbols]
11: functional layer；12: cover plate layer；111: cell liquid flow inlet；112: import liquid storage tank；113: flow channel is miniature
Dispersion column；114: bumper post；115: capture trap；116: the miniature dispersion column of flow pass；117: outlet liquid storage tank；118: waste liquor stream
Outlet.
Specific embodiment
In order to preferably explain the present invention, in order to understand, with reference to the accompanying drawing, by specific embodiment, to this hair
It is bright to be described in detail.
A kind of unicellular capture micro-fluidic chip is disclosed in the present embodiment as shown in Figure 1:, comprising: functional layer 11 and lid
Lamella 12.
Wherein, functional layer 11 includes: the multiple functional areas of silicon wafer ontology and modification on silicon wafer ontology；
Multiple functional areas described here include at least: sample feeding and pretreating zone, capturing function area and liquid waste processing
Area.
Specifically, the sample feeding in the present embodiment and pretreating zone by cell liquid flow inlet 111, import liquid storage tank 112,
It is provided with the flow channel composition of miniature dispersion column.
Capturing function area is formed by being provided with bumper post and capturing the minisize reaction pond of trap array；Liquid waste processing area is by being arranged
It is made of the flow pass of miniature dispersion column, outlet liquid storage tank 117 and waste liquid outflux 118；Cell liquid flow inlet 111 and import
Liquid storage tank 112 is connected to.
Import liquid storage tank 112 is connected to by means of flow channel with minisize reaction pond；Minisize reaction pond is by means of flow pass
It is connected to outlet liquid storage tank 117；Outlet liquid storage tank 117 is connected to waste liquid outflux 118；Cover plate layer 12 and functional layer 11 pass through envelope
Dress combination.
It is caught it is noted that micro-fluidic chip described in the present embodiment can be realized unicellular positioning in the chip
It obtains, passes through parameter setting, within the scope of the cell suspending liquid entrance velocity of 0.1~0.5 μ l/s, the cell capture efficiency of this chip
Reach 95% or more, after reaching Cell Homeostasis capture, it is effective that single sample consumption compares the culture of comparable sodium Tissue Culture Dish
Reduce by 90% or more.
Cell current-carrying liquid is crossed to cell liquid inflow entrance 111 all to enter in import liquid storage tank 112, then import liquid storage tank
Cell in 112 enters in minisize reaction pond by means of being provided with the flow channel of miniature dispersion column, by setting miniature anti-
115 array of capture trap in Ying Chi captures individual cells, and to analysis of experiments, remaining waste liquid enters outlet by flow pass
In liquid storage tank 117, the micro-fluidic chip finally is discharged by means of waste liquid outflux 118.
Should supplement: capturing function Qu You great array capture trap 115 described here forms, and can be realized high-throughput sample
Product transport and high efficiency cell capture；Capture array arrangement form can be adjusted according to concrete application, realize flexible group of chip
It closes, improves application.
It is as shown in Figure 2: import liquid storage tank 112, flow channel, minisize reaction pond, flow pass in the present embodiment and to go out
The mouth depth H 1 having the same of liquid storage tank 117；The miniature dispersion column 113 of flow channel, the miniature dispersion column 116 of flow pass, buffering
Column 114 and capture 115 array of trap height H2 having the same.
Capture array arrangement form can be adjusted according to concrete application, realize chip flexible combination, improve application.
It is noted that import liquid storage tank 112 described in the present embodiment and outlet liquid storage tank 117 can be set to circle
Or ellipse.
Miniature dispersion post shapes in the present embodiment in flow channel and flow pass are cylindrical body or Elliptic Cylinder；It is adjacent
Miniature dispersion column is shifted to install along flow liquid direction.
Here it should be noted that depth dimensions H1 and height dimension H2 in this embodiment example meet H1 > H2, and H1-H2
The diameter of < individual cells.
Above-mentioned bumper post is cylindrical body, semicylinder or Elliptic Cylinder.
114 quantity of bumper post of minisize reaction pond and flow channel communication port side is positioned close in the present embodiment by column
It is incremented by；For example first be classified as 1, second is classified as 2 or 3, and third is classified as 3 or 4 etc..
It is positioned close to minisize reaction pond and 114 quantity of bumper post of flow pass communication port side is successively decreased by column.With stream
The setting for entering channel communication port side is just arranged symmetrically.
115 array of trap is captured in the present embodiment to be made of each multiple capture traps 115 for arranging the arrangement that is staggered；
It captures trap 115 and is equipped with cellular retention area and carrying object guiding region.
As shown in Figure 3: cellular retention area lateral depth is H3 in the present embodiment, longitudinal width H4, and size meets H3 >=mono-
A cell dia, H4 > individual cells diameter；Carrying object guiding region is gap structure, and quantity N is 0~5, gap width H5；2
The diameter of μm < H5 < individual cells.
Specifically, at a plurality of gap, 180 ° of < of the initial 0 °≤θ of angle in two gaps of outermost.
Finally, it is noted that the material of functional layer 11 is dimethyl silicone polymer；The material of cover plate layer 12 is poly- diformazan
Radical siloxane or glass.
Implementation condition used in the examples can do further adjustment according to the condition of specific producer, the implementation being not specified
Condition is usually the condition in routine experiment.
In following example 1~3:
1, the preparation process of unicellular capture micro-fluidic chip is as follows:
(1) Wafer Cleaning: silicon wafer standard cleaning places 200 DEG C of electric hot plate 15min, drying；
(2) silicon wafer is modified: silicon wafer is placed in volatilization cylinder, instills 1~2 drop modification reagent HMDS
(Hexamethyldisilazane), volatilization processing time >=3min；
(3) silicon wafer whirl coating: on photoresist is poured on that treated silicon wafer, it is arranged according to required depth of pattern or height
Whirl coating revolving speed stands 1~2min after spin coating；
(4) silicon wafer exposure: front baking processing by determine the front baking time is set using the performance of photoresist；Exposure process
Need to consider that metering needed for exposure machine power and material carries out the setting time for exposure；After exposed, determination is taken out in template determination
It dries the time, baking processing in progress；
(5) develop: after cooling to be placed, putting it into developer solution；The specific time is arranged according to effect, develops in the process
It 2~3 times, places in ventilating kitchen, with being dried with nitrogen；
(6) release agent is handled: silicon wafer being placed in volatilization cylinder, 1~2 drop modification reagent tmcs (Trimethyl is instilled
Chlorosilane), volatilization is handled, and mold completes the process.
(7) prepared by PDMS glue: matching glue and spin coating；
(8) it modifies: the silicon wafer handled well is placed in volatilization cylinder, instilled and carried out with 1~2 drop dressing agent (methylchlorosilane)
Modification about 3min；
(9) it falls glue: being spread in ware using tinfoil, place silicon wafer mold, fall glue after silicon wafer is gently compacted, make silicon wafer
On glue bubble-free；
(10) dry: dry about 30min in 85 DEG C of thermostatic drying chambers；
(11) it shells glue: being taken out after slightly cooling down, remove tinfoil, carefully take PDMS off, the PDMS after solidification is separated with silicon wafer；
(12) cutting and punching: being cut along chip outline border with cutter, guarantee that chip structure is complete, punch punching；
(13) cleaning and microscopy: whether cleaning chip is qualified with micro- sem observation chip channel；
(14) bonding and quality inspection: plasma processor, processing need the chip surface that is bonded, and by processed two face paste
It closes, the bonding situation of procuratorial work chip under microscope；
(15) it toasts: being put into 65 DEG C of thermostatic drying chambers and toast overnight.
2, sample solution configures:
(1) cell liquid culture solution configure: RPMI-1640 culture medium, fetal calf serum, Pen .- Strep is dual anti-, three with
The volume capacity of 100:10:1 matches；
(2) cell extraction: taking out the HeLa cell of culture in carbon dioxide incubator, trypsase progress cell is added and disappears
Change, attached cell is separated, once used amount 2ml is centrifuged the mixed liquor of separator well, and 800r/min is centrifuged 3 points
Clock removes supernatant, and culture medium 4ml is added, obtains single experiment cell suspending liquid.
Embodiment 1
Prepare micro-fluidic chip (subsequent micro-fluidic chip be referred to as chip) in fashion described above, wherein dimensional parameters and
Other parameters are as follows: H1=H2=20 μm, H3=16 μm, H4=16 μm, H5=5 μm, N=0.Chip is successively passed through with syringe pump
Deionized water and phosphate buffered saline solution (PBS) are cleaned.Cell current-carrying liquid is passed through chip, control injection speed is 0.1 μ
Chip is placed in microscopically observation by l/s, finally realizes the unicellular capture in capture trap.Embodiment party through the invention
Formula 1, the unicellular capture rate in capture trap array reach 96%, and single sample consumption compares comparable sodium cell culture
Ware culture is effectively reduced 90% or more.
Embodiment 2
Micro-fluidic chip is prepared in fashion described above, wherein dimensional parameters and other parameters are as follows: H1=H2=20 μm, H3=16
μm, H4=16 μm, H5=5 μm, N=1.Chip is successively passed through to deionized water and phosphate buffered saline solution (PBS) with syringe pump
It is cleaned.Cell current-carrying liquid is passed through chip, control injection speed is 0.2 μ l/s, chip is placed in microscopically observation, most
The unicellular capture in capture trap is realized eventually.Embodiment 2 through the invention, the unicellular capture in capture trap array
Efficiency reaches 96%, and the comparison comparable sodium Tissue Culture Dish culture of single sample consumption is effectively reduced 95% or more.
Embodiment 3
Micro-fluidic chip is prepared in fashion described above, wherein dimensional parameters and other parameters are as follows: H1=H2=20 μm, H3=16
μm, H4=16 μm, H5=5 μm, N=2.Chip is successively passed through to deionized water and phosphate buffered saline solution (PBS) with syringe pump
It is cleaned.Cell current-carrying liquid is passed through chip, control injection speed is 0.2 μ l/s, chip is placed in microscopically observation, most
The unicellular capture in capture trap is realized eventually.Embodiment 3 through the invention, the unicellular capture in capture trap array
Efficiency reaches 98%, and the comparison comparable sodium Tissue Culture Dish culture of single sample consumption is effectively reduced 95% or more.
The technical principle of the invention is described above in combination with a specific embodiment, these descriptions are intended merely to explain of the invention
Principle shall not be construed in any way as a limitation of the scope of protection of the invention.Based on explaining herein, those skilled in the art
It can associate with other specific embodiments of the invention without creative labor, these modes fall within this hair
Within bright protection scope.
Claims (10)
1. a kind of unicellular capture micro-fluidic chip, which is characterized in that
It include: functional layer and cover plate layer；
Functional layer includes: the multiple functional areas of silicon wafer ontology and modification on silicon wafer ontology；
Multiple functional areas include at least: sample feeding and pretreating zone, capturing function area and liquid waste processing area；
Sample feeding and pretreating zone are by cell liquid flow inlet, import liquid storage tank, the flow channel group for being provided with miniature dispersion column
At；
Capturing function area is formed by being provided with bumper post and capturing the minisize reaction pond of trap array；
Liquid waste processing area is made of the flow pass, outlet liquid storage tank and waste liquid outflux for being provided with miniature dispersion column；
Cell liquid flow inlet is connected to import liquid storage tank；
Import liquid storage tank is connected to by means of flow channel with minisize reaction pond；
Minisize reaction pond is connected to by means of flow pass with outlet liquid storage tank；
Export liquid storage tank and waste liquor stream outlet；
Cover plate layer is combined with functional layer by encapsulation.
2. micro-fluidic chip according to claim 1, which is characterized in that
Import liquid storage tank, flow channel, minisize reaction pond, flow pass and outlet liquid storage tank depth H 1 having the same；
Miniature dispersion column, bumper post and capture trap array height H2 having the same.
3. micro-fluidic chip according to claim 1 or 2, which is characterized in that import liquid storage tank is circle with outlet liquid storage tank
Shape or ellipse.
4. micro-fluidic chip according to claim 2, which is characterized in that
Miniature dispersion post shapes in flow channel and flow pass are cylindrical body or Elliptic Cylinder；
Adjacent miniature dispersion column is shifted to install along flow liquid direction.
5. micro-fluidic chip according to claim 4, which is characterized in that depth dimensions H1 and height dimension H2 meet H1 >
H2, and the diameter of H1-H2 < individual cells.
6. micro-fluidic chip according to claim 1, which is characterized in that bumper post is cylindrical body, semicylinder or ellipse
Cylinder.
7. micro-fluidic chip according to claim 5, which is characterized in that be positioned close to minisize reaction pond and flow channel
The bumper post quantity of communication port side is incremented by by column；
It is positioned close to minisize reaction pond and the bumper post quantity of flow pass communication port side is successively decreased by column.
8. micro-fluidic chip according to claim 7, which is characterized in that capture trap array arranges the multiple of arrangement that are staggered by each
Capture trap composition；
It captures trap and is equipped with cellular retention area and carrying object guiding region.
9. micro-fluidic chip according to claim 8, which is characterized in that
Cellular retention area lateral depth is H3, longitudinal width H4, and size meets H3 >=individual cells diameter, and H4 > individual cells are straight
Diameter；
Carrying object guiding region is gap structure, and quantity N is 0~5, gap width H5；
The diameter of 2 μm of < H5 < individual cells；
At a plurality of gap, 180 ° of < of the initial 0 °≤θ of angle in two gaps of outermost.
10. micro-fluidic chip according to claim 9, which is characterized in that the material of functional layer is dimethyl silicone polymer；
The material of cover plate layer is dimethyl silicone polymer or glass.
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Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111349560A (en) * | 2020-03-16 | 2020-06-30 | 西北工业大学 | Micro-fluidic cell culture chip with self-amplification function |
| CN112067533A (en) * | 2020-09-26 | 2020-12-11 | 宁波大学 | Single cell mass spectrometry system and method |
| CN112076806A (en) * | 2019-06-14 | 2020-12-15 | 中国科学院青岛生物能源与过程研究所 | Centrifugal enrichment microfluidic chip for low-concentration liquid sample |
| CN112452364A (en) * | 2020-11-18 | 2021-03-09 | 江南大学 | Micro-fluidic chip for rapid sorting and manufacturing method |
| CN112547148A (en) * | 2020-12-09 | 2021-03-26 | 武汉纺织大学 | Micro-fluidic target capture chip based on micro dam array, preparation method and application |
| CN112574853A (en) * | 2020-12-14 | 2021-03-30 | 深圳先进技术研究院 | High-throughput micro-fluidic chip for pairing single-cell magnetic beads, pairing method and droplet array forming method |
| CN112916076A (en) * | 2021-01-22 | 2021-06-08 | 中国石油大学(华东) | Micro-fluidic chip experiment platform with visualization and infrared thermal imaging functions |
| CN112973986A (en) * | 2019-12-14 | 2021-06-18 | 深圳先进技术研究院 | Centrifugal device |
| CN113351264A (en) * | 2021-04-25 | 2021-09-07 | 上海交通大学 | Micro-fluidic adsorption chip for capturing circulating tumor cells by peripheral blood |
| CN113649096A (en) * | 2021-09-16 | 2021-11-16 | 苏州集微光电有限公司 | Exosome separation micro-fluidic chip and preparation method thereof |
| WO2022205399A1 (en) * | 2021-03-30 | 2022-10-06 | 苏州大学 | Integrated arrayed micro-fluidic chip for capturing and stretching cells |
| CN116121031A (en) * | 2022-12-23 | 2023-05-16 | 重庆大学 | Multistage microfluidic chip for single cell screening and preparation method thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104513787A (en) * | 2015-01-07 | 2015-04-15 | 东北大学 | Integrated micro-fluidic chip and system for capture, culture and administration of single cells |
| CN108949496A (en) * | 2017-05-18 | 2018-12-07 | 中国科学院大连化学物理研究所 | A kind of unicellular separation method based on drop micro-fluidic chip |
| CN108977343A (en) * | 2018-09-04 | 2018-12-11 | 哈尔滨工业大学 | The micro-fluidic chip separated for cell with capture based on dielectrophoresis principle |
-
2019
- 2019-04-09 CN CN201910281813.5A patent/CN110004043B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104513787A (en) * | 2015-01-07 | 2015-04-15 | 东北大学 | Integrated micro-fluidic chip and system for capture, culture and administration of single cells |
| CN108949496A (en) * | 2017-05-18 | 2018-12-07 | 中国科学院大连化学物理研究所 | A kind of unicellular separation method based on drop micro-fluidic chip |
| CN108977343A (en) * | 2018-09-04 | 2018-12-11 | 哈尔滨工业大学 | The micro-fluidic chip separated for cell with capture based on dielectrophoresis principle |
Non-Patent Citations (2)
| Title |
|---|
| JING ZHU等: ""A mechanically tunable microfluidic cell-trapping device"", 《SENSORS AND ACTUATORS A：PHYSICAL》 * |
| JINGYI XU等: ""A microfluidic chip with double-slit arrays for enhanced capture of single cells"", 《MICROMACHINES》 * |
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