CN109997809B - Enrichment and collection device and collection method for daphnia magna - Google Patents
Enrichment and collection device and collection method for daphnia magna Download PDFInfo
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- CN109997809B CN109997809B CN201910285035.7A CN201910285035A CN109997809B CN 109997809 B CN109997809 B CN 109997809B CN 201910285035 A CN201910285035 A CN 201910285035A CN 109997809 B CN109997809 B CN 109997809B
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- 241001494246 Daphnia magna Species 0.000 title claims abstract description 55
- 238000000034 method Methods 0.000 title claims abstract description 15
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 76
- 239000011324 bead Substances 0.000 claims abstract description 12
- 235000017166 Bambusa arundinacea Nutrition 0.000 claims description 21
- 235000017491 Bambusa tulda Nutrition 0.000 claims description 21
- 235000015334 Phyllostachys viridis Nutrition 0.000 claims description 21
- 241001330002 Bambuseae Species 0.000 claims description 20
- 239000011425 bamboo Substances 0.000 claims description 20
- 241000238578 Daphnia Species 0.000 description 19
- 239000011049 pearl Substances 0.000 description 9
- 210000004276 hyalin Anatomy 0.000 description 5
- 238000012360 testing method Methods 0.000 description 3
- 230000007547 defect Effects 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 241000143060 Americamysis bahia Species 0.000 description 1
- 241000209128 Bambusa Species 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 231100000671 aquatic toxicology Toxicity 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 231100000027 toxicology Toxicity 0.000 description 1
- 239000003643 water by type Substances 0.000 description 1
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K79/00—Methods or means of catching fish in bulk not provided for in groups A01K69/00 - A01K77/00, e.g. fish pumps; Detection of fish; Whale fishery
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K79/00—Methods or means of catching fish in bulk not provided for in groups A01K69/00 - A01K77/00, e.g. fish pumps; Detection of fish; Whale fishery
- A01K79/02—Methods or means of catching fish in bulk not provided for in groups A01K69/00 - A01K77/00, e.g. fish pumps; Detection of fish; Whale fishery by electrocution
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
The invention discloses an enrichment and collection device and a collection method of daphnia magna, which comprise the following steps: the two opening and closing cylinders are mutually connected in a back-to-back mode and fixed together through a connecting piece, each opening and closing cylinder comprises two opening and closing plates which are hinged with each other, and an opening and closing opening is formed in each opening and closing cylinder along the axial direction of the opening and closing cylinder; the opening and closing cylinder is axially provided with at least one collecting cavity, and the inner wall of the collecting cavity is provided with at least one lamp bead; one end of each of the two opening and closing cylinders is connected with a water suction pipe, and the opening and closing cylinders can be closed when the water suction pipes suck water.
Description
Technical Field
The invention relates to a collecting device, in particular to an enrichment collecting device and method for daphnia magna.
Background
The daphnia magna is commonly used in eutrophic small water areas with luxuriant aquatic weeds, such as ponds, water pits, intermittent ponding, small lakes and the like, and can also live in water with high salinity, and the daphnia magna has the advantages of short life cycle, quick reproduction, economy, easy laboratory culture and the like and has an important position in a water area ecosystem, so that the daphnia magna is valued by numerous national toxicology workers, is high in sensitivity, is a very representative plankton, is a standard test organism, and is widely applied to research of aquatic toxicology.
The laboratory and the outdoor collection large-scale daphnia are more troublesome, and individual difference is great, also can carry a large amount of shrimps simultaneously in outdoor collection process, or other aquatic creatures, influence the experimental data accuracy of large-scale daphnia, and traditional collection method can not realize the enrichment collection of large-scale daphnia, and can not carry out contrast collection, experiment to large-scale daphnia, and the practicality is not strong.
Disclosure of Invention
The invention overcomes the defects of the prior art and provides the enrichment and collection device and the collection method of daphnia magna.
In order to achieve the purpose, the invention adopts the technical scheme that: an enrichment collection system of daphnia magna, includes: the two opening and closing cylinders are mutually connected in a back-to-back mode and fixed together through a connecting piece, each opening and closing cylinder comprises two opening and closing plates which are hinged with each other, and an opening and closing opening is formed in each opening and closing cylinder along the axial direction of the opening and closing cylinder; the opening and closing cylinder is axially provided with at least one collecting cavity, and the inner wall of the collecting cavity is provided with at least one lamp bead; one end of each of the two opening and closing cylinders is connected with a water suction pipe, and the opening and closing cylinders can be closed when the water suction pipes suck water.
In a preferred embodiment of the invention, the joint piece is arranged along the axis of the opening and closing cylinder, and the opening and closing cylinder can rotate along the axial direction of the joint piece.
In a preferred embodiment of the invention, the brightness of the lamp beads arranged in the two opening and closing cylinders can be the same or different.
In a preferred embodiment of the invention, a bait is suspended in the collection chamber.
In a preferred embodiment of the invention, a plurality of transparent tubes are arranged on the opening plate at intervals corresponding to the edges of the opening and closing openings.
In a preferred embodiment of the present invention, the two opening plates on the same opening and closing cylinder are respectively provided with a plurality of transparent tubes in a staggered arrangement.
In a preferred embodiment of the present invention, the transparent tube has an arc-shaped structure, and the inner and outer sides of the arc-shaped transparent tube are consistent with the inner and outer sides of the arc-shaped opening plate.
In a preferred embodiment of the invention, the water pump is arranged in the middle of the water suction pipe, one end of the water suction pipe is arranged at one end of the opening and closing cylinder, and the other end of the water suction pipe is provided with the mesh bag.
In a preferred embodiment of the invention, the aperture of the mesh bag is smaller than the size of the daphnia magna to be collected.
In a preferred embodiment of the invention, a bar-shaped light bar is arranged in the transparent tube.
In a preferred embodiment of the invention, the closed free ends of the two opening and closing cylinders form a conical structure.
In order to achieve the purpose, the invention adopts another technical scheme as follows: a method for collecting daphnia magna comprises the following steps:
(1) placing the collecting device in water, turning on the lamp beads and the strip-shaped lamp rods in the transparent tubes, and attracting the daphnia magna around to be close to the inner side of the opening and closing cylinder through light and bait;
(2) after the collecting device lures the daphnia magna in water for a period of time, the two open-close plates surround to form a closed collecting cavity through the suction of the water suction pipe, and the daphnia magna is sealed in the collecting cavity;
(3) the water sucked by the water suction pipe is filtered by the mesh bag, and the daphnia magna is collected in the mesh bag.
In a preferred embodiment of the invention, when the daphnia magna with different body states needs to be collected, the brightness of the first lamp bead and the brightness of the second lamp bead are adjusted to be different, and the two water suction pipes are respectively connected with the two opening and closing cylinders for suction.
In a preferred embodiment of the invention, the two suction pipes are respectively connected with mesh bags with different mesh sizes.
The invention solves the defects in the background technology, and has the following beneficial effects:
(1) draw water to the top of every section of thick bamboo that opens and shuts through water pump and suction pipe, according to Bernoulli's theorem, two open-close boards begin the closure promptly when drawing water, and the section of thick bamboo that opens and shuts changes into the closed enclosure space suction state that is from the luring state that opens and shuts mouthful luring the daphnia into to block the daphnia in the enclosure space, do not need external power just can be with the section of thick bamboo closure that opens and shuts, simple to use.
(2) At the pipe that absorbs water and the water pump in-process that absorbs water, the daphnia magna is most likely to escape from the opening and shutting, and the effect of absorbing water this moment leads to rivers can flow into from the opening and collect the chamber, and the escape of daphnia magna is escaped for the counter current direction this moment, and it is more difficult to escape, and this kind of mode can greatly reduce the quantity of escaping of daphnia magna in the closed process.
(3) The free end of the opening and closing cylinder with the conical structure can increase the water flow speed of the collecting cavity far away from one end of the water suction pipe when the opening and closing cylinder is closed, so that the water flow speed of the opening and closing cylinder from the free end to the direction of the water suction pipe is gradually increased, the daphnia magna is guaranteed to be difficult to escape from the opening of the free end of the opening and closing cylinder, and the daphnia magna is smoothly sucked away by the water suction pipe to enter the mesh bag.
(4) The transparent pipes at the two ends of the two opening and closing cylinders are alternately distributed, and after the opening and closing cylinders are closed, the spaces can be pre-closed by the alternately distributed transparent pipes, so that daphnia magna entering the enclosed spaces cannot escape again.
(5) Two cylinders that open and shut can adjust the lamp pearl luminance of the collection intracavity of usefulness respectively, when the large-scale daphnia of different somatotypes was gathered to needs, the luminance of intracavity lamp pearl is collected separately in the adjustment, and two kinds of lamp pearls of luminance difference can realize collecting the large-scale daphnia somatotype variation that the intracavity was gathered to carry out contrast test to large-scale daphnia.
(6) When the daphnia magna of different posture is gathered to needs, two section of thick bamboo one end that open and shut are sucked through two water pumps respectively, and the pocket that corresponds the aperture is connected to suction tube one end, can collect the daphnia magna of different posture.
(7) After the opening and closing cylinder is closed, the free end forms a conical structure, and the arrangement of the conical structure can ensure that when the water pump sucks water, the caliber of the opening and closing cylinder is changed from small to large, so that the water pressure difference of two sides can be formed, the daphnia magna in the opening and closing cylinder cannot run out through the conical opening at the bottom, and the safety and reliability are higher.
(8) The transparent tubes distributed alternately can form a closed space, can enlarge the attracting light distribution range of the collection area of the opening and closing cylinder, and attracts daphnia magna in a wider water area, so that the area of the collection area can be increased, and enrichment collection of daphnia magna is realized.
Drawings
The invention is further explained below with reference to the figures and examples;
FIG. 1 is a partial perspective block diagram of a preferred embodiment of the present invention;
FIG. 2 is a schematic view of the structure of the opening and closing cylinder of the preferred embodiment of the present invention;
FIG. 3 is a front view of a partial structure of a preferred embodiment of the present invention;
in the figure: 1. a suction pipe; 2. a transparent tube; 3. a collection chamber; 4. an opening and closing cylinder; 5. a lamp bead; 6. opening and closing the board; 7. a connecting piece.
Detailed Description
The invention will now be described in further detail with reference to the accompanying drawings and examples, which are simplified schematic drawings and illustrate only the basic structure of the invention in a schematic manner, and thus show only the constituents relevant to the invention.
As shown in fig. 1-3, an enrichment and collection device for daphnia magna comprises: the two opening and closing cylinders 4 are mutually connected in a back-to-back mode, the two opening and closing cylinders 4 are fixed together through a connecting piece 7, each opening and closing cylinder 4 comprises two opening and closing plates 6 which are hinged with each other, and the opening and closing cylinders 4 form opening and closing openings along the axial direction of the opening and closing cylinders 4; at least one collecting cavity 3 is axially arranged on the opening and closing cylinder 4, and a lamp bead 5 is arranged on the inner wall of the collecting cavity 3; one end of each of the two opening and closing cylinders 4 is connected with a water suction pipe 1, and the opening and closing cylinders 4 can be closed when the water is sucked through the water suction pipes 1.
Linking member 7 sets up along a 4 axes that open and shut, and a 4 can be followed the axial rotation of linking member 7 to open and shut, and the luminance of the lamp pearl 5 that sets up respectively in two a 4 section of thick bamboos that open and shut can be the same or different, and the collection intracavity has hung the bait.
The edge that corresponds the division board 6 and opens and shuts the mouth sets up a plurality of hyaline tube 2 alternately, install the bar lamp stick in the hyaline tube 2, the crisscross setting in a plurality of hyaline tube 2 position that two division boards 6 on the same section of thick bamboo 4 of opening and shutting correspond respectively, 4 closed backs of the section of thick bamboo of opening and shutting, alternate distribution's hyaline tube 2 can be with the space pre-closure, can not make the large-scale daphnia in getting into the enclosed space escape once more, alternate distribution's hyaline tube 2 can form the closed space, can enlarge the collection area's of the section of thick bamboo 4 that opens and shuts lures light distribution range again, attract the large-scale daphnia in wider waters, thereby can improve the area of collection area, realize the enrichment collection of large-scale daphnia.
The transparent tube 2 is arc-shaped, and the arc inner and outer sides of the transparent tube 2 are consistent with the arc inner and outer sides of the opening plate 6.
The pipe 1 middle part that absorbs water sets up the water pump, 1 one end setting of the pipe that absorbs water is in a section of thick bamboo 4 one end that opens and shuts, the pipe 1 other end that absorbs water sets up the pocket, the pocket aperture is less than the large-scale daphnia size that needs to be collected, through the water pump with absorb water pipe 1 and draw water to every top of a section of thick bamboo 4 that opens and shuts, according to Bernoulli's theorem, two open-close boards 6 are the closure promptly during draw water, the section of thick bamboo 4 that opens and shuts changes into the closed space suction state that surrounds that is from the luring state that the opening and shutting mouthful lures large-scale daphnia, thereby block large-scale daphnia in the space that surrounds, do not need external power just can be closed with a section of thick bamboo 4 that opens and shuts, and is simple to use.
At the pipe 1 that absorbs water and the water pump in-process that absorbs water, the daphnia magna is most likely to escape from the opening and shutting, and the effect of absorbing water this moment leads to rivers to flow into from the opening and collects chamber 3, and the escape of daphnia magna is the counter current direction escape this moment, and the escape is more difficult, and this kind of mode can greatly reduce the quantity of escaping of daphnia magna in the closed process.
The free ends of the two opening and closing cylinders 4 after being closed form a conical structure, the free ends of the opening and closing cylinders 4 form a conical structure after the opening and closing cylinders 4 are closed, and the arrangement of the conical structure can ensure that when a water pump sucks water, the calibers of the opening and closing cylinders 4 are changed from small to large, water pressure difference on two sides can be formed, daphnia magna in the opening and closing cylinders 4 cannot run out through the conical openings in the bottoms, and the safety and reliability are high.
The 4 free ends of a section of thick bamboo that opens and shuts of toper structure can increase when a section of thick bamboo 4 is closed opening and shutting, and the water velocity of collecting chamber 3 is kept away from to 1 one end of water-absorbing pipe for a section of thick bamboo 4 that opens and shuts increases gradually from the free end to 1 direction water velocity of water-absorbing pipe, guarantees that daphnia magna also is difficult to escape from opening of the 4 free ends of a section of thick bamboo that opens and shuts, and is absorbed away the entering pocket smoothly by water-absorbing pipe 1.
In order to achieve the purpose, the invention adopts another technical scheme as follows: a method for collecting daphnia magna comprises the following steps:
(1) placing the collecting device in water, turning on the lamp beads 5 and the bar-shaped lamp rods in the transparent tubes 2, and attracting the daphnia magna around to be close to the inner side of the opening and closing cylinder 4 through light and bait;
(2) after the collecting device lures daphnia magna in water for a period of time, the collecting device sucks water through the water suction pipe 1, the two split plates 6 surround to form a closed collecting cavity 3, and the daphnia magna is sealed in the collecting cavity 3 at the moment;
(3) the water sucked by the water suction pipe 1 is filtered by the mesh bag, and the daphnia magna is collected in the mesh bag.
When the large-scale daphnia of different somatotypes is gathered to needs, the luminance of adjustment first lamp pearl 5 and second lamp pearl 5 is different, and absorb water pipe 1 through two and connect two section of thick bamboo 4 that open and shut respectively and aspirate, two section of thick bamboo 4 that open and shut can adjust the lamp pearl 5 luminance in the collection chamber 3 with respectively, when the large-scale daphnia of different somatotypes is gathered to needs, adjust the luminance of collecting 3 interior lamp pearls of chamber 5 separately, the large-scale daphnia somatotype size difference of gathering in the chamber 3 can be realized collecting to two kinds of lamp pearls 5 that luminance is different, thereby carry out contrast test to large-scale daphnia.
When the daphnia magna of different posture is gathered to needs, two 4 one ends of the section of thick bamboo that opens and shuts are sucked respectively through two water pumps, are connected with the pocket of different mesh sizes on two pipes 1 that absorb water respectively, can collect the daphnia magna of different posture.
In light of the foregoing description of the preferred embodiment of the present invention, it is to be understood that various changes and modifications may be made by one skilled in the art without departing from the spirit and scope of the invention. The technical scope of the present invention is not limited to the content of the specification, and must be determined according to the scope of the claims.
Claims (10)
1. An enrichment collection system of daphnia magna, includes: the folding device comprises two folding cylinders which are mutually connected in a back-to-back manner, and the two folding cylinders are fixed together through a connecting piece, and is characterized in that each folding cylinder comprises two folding plates which are mutually hinged, and the folding cylinders axially form folding openings along the folding cylinders;
the opening and closing cylinder is axially provided with at least one collecting cavity, and the inner wall of the collecting cavity is provided with at least one lamp bead;
one end of each of the two opening and closing cylinders is connected with a water suction pipe, and when the water is sucked through the water suction pipes, the opening and closing cylinders can be closed by pressure difference formed by water flow.
2. The enrichment and collection device for daphnia magna according to claim 1, characterized in that: the link piece is followed a section of thick bamboo axis setting opens and shuts, a section of thick bamboo that opens and shuts can be followed the axial rotation of link piece.
3. The enrichment and collection device for daphnia magna according to claim 1, characterized in that: the brightness of the lamp beads respectively arranged in the two opening and closing cylinders can be the same or different.
4. The enrichment and collection device for daphnia magna according to claim 1, characterized in that: bait is hung in the collecting cavity.
5. The enrichment and collection device for daphnia magna according to claim 1, characterized in that: and a plurality of transparent tubes are arranged on the opening plate at intervals corresponding to the edges of the opening and closing openings.
6. The enrichment and collection device for daphnia magna according to claim 5, wherein: the two opening plates on the same opening and closing cylinder are respectively arranged in a staggered mode in the corresponding positions of the plurality of transparent tubes.
7. The device for gathering and collecting daphnia magna according to claim 5 or 6, wherein: and a strip-shaped lamp rod is arranged in the transparent tube.
8. The enrichment and collection device for daphnia magna according to claim 1, characterized in that: and the free ends of the two opening and closing cylinders after being closed form a conical structure.
9. A method for collecting daphnia magna according to claim 1, comprising the steps of: (1) placing the collecting device in water, turning on the lamp beads and the strip-shaped lamp rods in the transparent tubes, and attracting the daphnia magna around to be close to the inner side of the opening and closing cylinder through light and bait;
(2) after the collecting device lures the daphnia magna in water for a period of time, the two open-close plates surround to form a closed collecting cavity through the suction of the water suction pipe, and the daphnia magna is sealed in the collecting cavity;
(3) the water sucked by the water suction pipe is filtered by the mesh bag, and the daphnia magna is collected in the mesh bag.
10. The method for collecting daphnia magna according to claim 9, wherein: when needing to gather the daphnia magna of different posture, the luminance of adjustment lamp pearl, and connect two cylinders that open and shut respectively through two pipes that absorb water and suck.
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| CN201910285035.7A CN109997809B (en) | 2019-04-10 | 2019-04-10 | Enrichment and collection device and collection method for daphnia magna |
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| CN106417199B (en) * | 2016-10-09 | 2022-07-26 | 华南农业大学 | Fish integrated equipment is received to stator propelling movement formula in cylinder |
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