CN109997696A - The rapid propagation method of succulent jade fan - Google Patents
The rapid propagation method of succulent jade fan Download PDFInfo
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- CN109997696A CN109997696A CN201910300804.6A CN201910300804A CN109997696A CN 109997696 A CN109997696 A CN 109997696A CN 201910300804 A CN201910300804 A CN 201910300804A CN 109997696 A CN109997696 A CN 109997696A
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- callus
- medium
- culture medium
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- fan
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
Abstract
The invention belongs to field of plant growing technology, are related to a kind of rapid propagation method of succulent jade fan.Using method of the invention, the callus induction rate of succulent jade fan is up to 90%, and average rooting rate is up to 90%.
Description
Technical field
The invention belongs to field of plant growing technology, are related to a kind of rapid propagation method of succulent jade fan.
Background technique
Succulent comes in every shape because various in style, the small and exquisite grace of plant, color multiplicity, adaptable, in recent years by
People's likes.Succulent in addition to very high ornamental value, contain huge market potential other than, also have edible, medicinal
And a variety of application values such as environmental protection.The coefficient that succulent is bred by modes such as sowing, cuttage, grafting, plant division
It is very low, it is not often able to satisfy market and the demand of people.Currently, by tissue culture technique, fast breeding new plant has become
The research hotspot of succulent fast breeding.
Jade fan (Haworthia truncata) is also referred to as section shape volume 12, belongs to a kind of microphyll fleshiness for Liliaceae volume 12
Plant originates in South Africa Cape province.Jade fans no stem, and leaf green, plump, uprightly, top is bent slightly inwards；Meat blade 8-
It is 12 or so, fan-shaped in one line；It is in dirty-green, rough surface after the irradiation of vanes sunlight.There is section on the top of blade, claims
Make " window ", it is often in canescence that the transparency of " window " is high, and sunlight enters from " window " carries out photosynthesis in plant body.Plant exists
Meeting pumping goes out bennet during summer and autumn, and high about 30cm, flower is smaller, white, total shape scape, and root system is sturdy.
Jade fan is lived in the environment of warm arid, drought-resistant, can not resist cold, and happiness is sufficient and soft sunlight, be afraid of high temperature with
Strong light direct beam.As most of succulent, jade fan can enter of short duration dormant period in temperature higher summer, and growth is slow
Slowly it or stops growing.This period, jade fan do not need excessive moisture, are placed on ventilation shading maintenance.Spring is beautiful
The animated period for fanning growth needs sufficient illumination, and keeps in basin ground moistening but not ponding.If illumination is insufficient, blade is elongated,
Misaligned, plant is loose.If moisture stays on blade face for a long time, especially the position of Central growing point, blade is easy corruption
It is rotten.
There are many gardening kind of jade fan, and section is other than common ellipse, there are also U-typed and " M " type etc. are a variety of, grow
Degree, width, thickness and the transparency in section have very big difference.Greenstone fan, a kind of variety of jade fan, blade is relatively thin,
Aquamarine, but the transparency of blade profile is poor.Jade fan brocade belongs to the garden-variety of beautiful fan, has yellow or powder on blade profile
Flammulation is of great rarity and beautiful.According to the difference of decorative pattern, leaf color and plant forms, jade fan difference in price is outstanding
Very.Often compact with blade plumpness, arrangement, the higher person of the transparency in section is considered as top grade.Jade fan plant shape is seemingly fanned, small and exquisite grace,
The transparent such as window in section, decorative pattern is changeable, has high ornamental values and the economic values.
Liliaceous beautiful fan becomes the precious product that numerous gardening enthusiasts collect with its unique shape, decorative pattern and color
Kind.As most of succulent, there are many kinds of the modess of reproduction of jade fan: cuttage, grafting, plant division and sowing, but
Their slow growth and breeding coefficient is small, reproduction speed is slow under natural conditions, can be in short cycle using tissue culture technique
It is interior to obtain a large amount of new plant.Currently, tissue culture and fast numerous research in relation to succulent have more report, but fanned about jade
There is not been reported for kind tissue cultures and rapid propagation system research.With being continuously increased for the market demand, jade is fanned under natural conditions
Breeding can no longer meet the demand in market, thus to it is beautiful fan carry out tissue cultures be very it is necessary to, both meet market
Demand, and abundant and perfect succulent tissue culture and rapid proliferation technical data.
Summary of the invention
This method is fanned using Liliaceae succulent jade as research object, is carried out respectively using scape and blade as explant
Callus induction, Multiplying culture, bud induction and shoot proliferation culture, culture of rootage and hardening and transplanting domestication, are opened respectively
The selection for having opened up induced medium, proliferated culture medium and root media has studied different plant hormones or growth regulator group
It closes, influence of the different condition of culture to callus induction and proliferation, inducing clumping bud and proliferation and rooting of vitro seedling, tentatively
The tissue culture quick breeding technical system for establishing beautiful fan, provides theoretical foundation and technical system for its industrial seedling rearing, is later
The researchs such as breed improvement lay the foundation.
The first aspect of the present invention provides a kind of rapid propagation method of succulent jade fan, the specific steps of this method
Are as follows:
(1) selection and disinfection of explant: beautiful fan explant is chosen, cleans and sterilizes spare；
(2) induction and proliferation of callus: the explant after disinfection is cut into segment, inoculation is gently inserted in callus and lures
It leads in culture medium, is subsequently placed in callus proliferation medium, obtain callus；Wherein callus inducing medium
Component are as follows: using MS as minimal medium, 0.1mg/L methyl α-naphthyl acetate, 2.0mg/L 6- benzyl aminoadenine are added in every liter of culture medium；
The component of callus proliferation medium are as follows: using MS as minimal medium, 0.05mg/L methyl α-naphthyl acetate is added in every liter of culture medium,
2.0mg/L Thidiazuron；
(3) callus acquired in step (2) induction and proliferation of Multiple Buds: is transferred to the induction and proliferation of Multiple Buds
In culture medium, induction generates adventitious bud；The wherein component of inducing clumping bud and proliferated culture medium are as follows: using MS as minimal medium,
0.01mg/L methyl α-naphthyl acetate, 0.2mg/L Thidiazuron are added in every liter of culture medium；
(4) Multiple Buds are taken root: culture of rootage are carried out by being transferred in root media caused by step (3), to obtain
Intact plant；The wherein component of root media are as follows: using 1/2MS as minimal medium, 0.3mg/L naphthalene is added in every liter of culture medium
Acetic acid；
The condition of culture of above each step is equal are as follows: 25 ± 2 DEG C of temperature, humidity 80%-95%, intensity of illumination is
2000lux, incubation time 30-60d, periodicity of illumination 10-14hd-1。
In one embodiment, the beautiful fan explant is scape.
In one embodiment, the beautiful fan explant is blade.
In one embodiment, the step (1) specifically: choose beautiful fan scape as explant, rinsed with clear water
The attachment on explant surface, then 5min is impregnated with 2% abluent solution, a few hours are rinsed through tap water；It is subsequently placed in super
15s is impregnated with 75% alcohol on net workbench and is constantly shaken, aseptic water washing 1-2 times, then is impregnated with 0.1% mercuric chloride
15min simultaneously constantly shakes, aseptic water washing 4-5 times, and it is standby that the material handled well is finally placed on to suck dry moisture on sterile filter paper
With.
In another embodiment, the step (1) specifically: choose beautiful fan leaf as explant, rushed with clear water
The attachment on explant surface is washed, then impregnates 5min with 2% abluent solution, rinses a few hours through tap water；It is subsequently placed in
15s is impregnated with 75% alcohol on superclean bench and is constantly shaken, aseptic water washing 1-2 times, then is soaked with 0.1% mercuric chloride
Bubble 15min simultaneously constantly shakes, aseptic water washing 4-5 times, and the material handled well is finally placed on to suck dry moisture on sterile filter paper
It is spare.
In one embodiment, the step (2) specifically: the scape after disinfection is cut into the segment of 0.5cm, section
Inoculation is gently inserted in callus inducing medium downward, to obtain callus.The component of callus inducing medium are as follows:
Using MS as minimal medium, 0.1mg/L methyl α-naphthyl acetate, 2.0mg/L 6- benzyl aminoadenine are added in every liter of culture medium.Callus group
Knit the component of proliferated culture medium are as follows: using MS as minimal medium, 0.05mg/L methyl α-naphthyl acetate, 2.0mg/L are added in every liter of culture medium
Thidiazuron.
In another embodiment, the step (2) specifically: the blade after disinfection is cut into the small of 0.5 × 0.5cm
Block, section is inoculated with downward to be gently inserted in callus inducing medium, to obtain callus.Callus inducing medium
Component are as follows: using MS as minimal medium, 0.1mg/L methyl α-naphthyl acetate, 2.0mg/L 6- benzyl aminoadenine are added in every liter of culture medium.
The component of callus proliferation medium are as follows: using MS as minimal medium, 0.05mg/L methyl α-naphthyl acetate is added in every liter of culture medium,
2.0mg/L Thidiazuron.
The beautiful fan that the method that the second aspect of the present invention provides according to claim 1 any one of -6 obtains.
The present invention obtains succulent seedling, this method materials disinfection side by carrying out tissue cultures to succulent
Just, bud induction rate, rooting rate, survival rate are higher, with short production cycle, improve succulent breeding coefficient and reproduction speed,
Meet production requirement.
Detailed description of the invention
Fig. 1 is the callus of beautiful fan scape.
Fig. 2 is the bud induction of beautiful fan scape.
Fig. 3 is the bud proliferation of beautiful fan scape.
Fig. 4 is taking root for beautiful fan.
Specific embodiment
Embodiment
Embodiment 1
Choosing beautiful fan scape is explant, rinses the attachment on explant surface with clear water, then with 2% abluent solution
5min is impregnated, rinses a few hours through tap water；It is subsequently placed in and impregnates 15s with 75% alcohol on superclean bench and constantly shake
It is dynamic, aseptic water washing 1-2 times, then impregnate 15min with 0.1% mercuric chloride and constantly shake, aseptic water washing 4-5 times, finally will
It is spare that the material handled well is placed on suck dry moisture on sterile filter paper.The induction and proliferation of callus: by the flower of sterile beautiful fan
Roripa is cut into the segment of 0.5cm, and downward, every bottle is inoculated with 1 section, is gently inserted in callus inducing medium for section, is inoculated with 20 bottles altogether,
To obtain callus.The component of callus inducing medium are as follows: using MS as minimal medium, be added in every liter of culture medium
0.1mg/L methyl α-naphthyl acetate, 2.0mg/L 6- benzyl aminoadenine.The component of callus proliferation medium are as follows: trained with MS to be basic
Base is supported, 0.05mg/L methyl α-naphthyl acetate, 2.0mg/L Thidiazuron is added in every liter of culture medium.
The induction and proliferation of Multiple Buds: generated green, graininess callus are transferred to the Fiber differentiation of Multiple Buds
In base, induction generates adventitious bud.The component of inducing clumping bud and proliferated culture medium are as follows: using MS as minimal medium, every liter of culture
0.01mg/L methyl α-naphthyl acetate, 0.2mg/L Thidiazuron are added in base.
Multiple Buds are taken root: the Multiple Buds being transferred to culture of rootage in root media, to obtain intact plant.It takes root
The component of culture medium are as follows: using 1/2MS as minimal medium, 0.3mg/L methyl α-naphthyl acetate is added in every liter of culture medium.
Condition of culture is equal are as follows: 25 ± 2 DEG C of temperature, humidity 80%-95%, intensity of illumination 2000lux, incubation time is
30-60d, periodicity of illumination 12hd-1。
Finally, 18 bottles of scape callus are successfully induced altogether, wherein successfully taking root 18 bottles, are fanned using the jade of the method for the present invention
Scape callus induction rate is up to 90%, and average rooting rate is up to 90%.
Embodiment 2
Choosing beautiful fan leaf is explant, rinses the attachment on explant surface with clear water, then with 2% abluent solution
5min is impregnated, rinses a few hours through tap water；It is subsequently placed on superclean bench and impregnates aseptic water washing 4-5 with 75% alcohol
It is secondary, it is spare that the material handled well is finally placed on to suck dry moisture on sterile filter paper.The induction and proliferation of callus: will be sterile
The blade of jade fan is cut into the fritter of 0.5 × 0.5cm, and downward, every bottle is inoculated with 1 piece, and inoculation is gently inserted in callus induction training for section
It supports in base, is inoculated with 20 bottles, altogether to obtain callus.The component of callus inducing medium are as follows: using MS as minimal medium,
0.1mg/L methyl α-naphthyl acetate, 2.0mg/L 6- benzyl aminoadenine are added in every liter of culture medium.The component of callus proliferation medium
Are as follows: using MS as minimal medium, 0.05mg/L methyl α-naphthyl acetate, 2.0mg/L Thidiazuron are added in every liter of culture medium.It will be generated green
Color, graininess callus are transferred in the induced medium of Multiple Buds, and induction generates adventitious bud.Inducing clumping bud and Multiplying culture
The component of base are as follows: MS is minimal medium, and 0.01mg/L methyl α-naphthyl acetate, 0.2mg/L Thidiazuron is added in every liter of culture medium.It will be described
Multiple Buds are transferred to culture of rootage in root media, to obtain intact plant.The component of root media are as follows: using 1/2MS as base
0.3mg/L methyl α-naphthyl acetate is added in every liter of culture medium for basal culture medium.
Condition of culture is equal are as follows: 25 ± 2 DEG C of temperature, humidity 80%-95%, intensity of illumination 2000lux, incubation time is
30-60d, periodicity of illumination 12hd-1。
Finally, succeed 13 bottles of inducer blade callus altogether, wherein successfully taking root 17 bottles, is fanned using the jade of the method for the present invention
Callus of Leaf inductivity is up to 65%, and average rooting rate is up to 85%.
The Applicant declares that the present invention is explained by the above embodiments method detailed of the invention, but the present invention not office
Be limited to above-mentioned method detailed, that is, do not mean that the invention must rely on the above detailed methods to implement.Technical field
Technical staff it will be clearly understood that any improvement in the present invention, equivalence replacement and auxiliary element to each raw material of product of the present invention
Addition, selection of concrete mode etc., all of which fall within the scope of protection and disclosure of the present invention.
Claims (8)
1. a kind of rapid propagation method of succulent jade fan, the specific steps of this method are as follows:
(1) selection and disinfection of explant: beautiful fan explant is chosen, cleans and sterilizes spare；
(2) induction and proliferation of callus: the explant after disinfection is cut into segment, inoculation is gently inserted in callus induction training
It supports in base, is subsequently placed in callus proliferation medium, obtain callus；The wherein component of callus inducing medium
Are as follows: using MS as minimal medium, 0.1mg/L methyl α-naphthyl acetate, 2.0mg/L6- benzyl aminoadenine are added in every liter of culture medium；Callus
The component of hyperblastosis culture medium are as follows: using MS as minimal medium, 0.05mg/L methyl α-naphthyl acetate, 2.0mg/ are added in every liter of culture medium
L Thidiazuron；
(3) callus acquired in step (2) induction and proliferation of Multiple Buds: is transferred to induction and the Multiplying culture of Multiple Buds
In base, induction generates adventitious bud；The wherein component of inducing clumping bud and proliferated culture medium are as follows: using MS as minimal medium, every liter
0.01mg/L methyl α-naphthyl acetate, 0.2mg/L Thidiazuron are added in culture medium；
(4) Multiple Buds are taken root: culture of rootage is carried out by being transferred in root media caused by step (3), it is complete to obtain
Plant；The wherein component of root media are as follows: using 1/2MS as minimal medium, 0.3mg/L naphthalene second is added in every liter of culture medium
Acid；
The condition of culture of above each step is equal are as follows: and 25 ± 2 DEG C of temperature, humidity 80%-95%, intensity of illumination 2000lux, training
Supporting the time is 30-60d, periodicity of illumination 10-14hd-1。
2. the method as described in claim 1, wherein the beautiful fan explant is scape.
3. the method as described in claim 1, wherein the beautiful fan explant is blade.
4. method as claimed in claim 2, the step (1) specifically: choose beautiful fan scape as explant, rinsed with clear water outer
The attachment on implant surface, then 5min is impregnated with 2% abluent solution, a few hours are rinsed through tap water；It is subsequently placed in ultra-clean
15s is impregnated with 75% alcohol on workbench and is constantly shaken, aseptic water washing 1-2 times, then is impregnated with 0.1% mercuric chloride
15min simultaneously constantly shakes, aseptic water washing 4-5 times, and it is standby that the material handled well is finally placed on to suck dry moisture on sterile filter paper
With.
5. method as claimed in claim 3, the step (1) specifically: choose beautiful fan leaf as explant, rinsed with clear water outer
The attachment on implant surface, then 5min is impregnated with 2% abluent solution, a few hours are rinsed through tap water；It is subsequently placed in ultra-clean
15s is impregnated with 75% alcohol on workbench and is constantly shaken, aseptic water washing 1-2 times, then is impregnated with 0.1% mercuric chloride
15min simultaneously constantly shakes, aseptic water washing 4-5 times, and it is standby that the material handled well is finally placed on to suck dry moisture on sterile filter paper
With.
6. method as claimed in claim 2, the step (2) specifically: the scape after disinfection is cut into the segment of 0.5cm, section
Inoculation is gently inserted in callus inducing medium downward, to obtain callus；The component of callus inducing medium are as follows:
Using MS as minimal medium, 0.1mg/L methyl α-naphthyl acetate, 2.0mg/L6- benzyl aminoadenine are added in every liter of culture medium；Callus
The component of proliferated culture medium are as follows: using MS as minimal medium, 0.05mg/L methyl α-naphthyl acetate, 2.0mg/L thiophene are added in every liter of culture medium
Benzene is grand.
7. method as claimed in claim 3, the step (2) specifically: the blade after disinfection is cut into the fritter of 0.5 × 0.5cm,
Section is inoculated with downward to be gently inserted in callus inducing medium, to obtain callus；The group of callus inducing medium
It is divided into: using MS as minimal medium, 0.1mg/L methyl α-naphthyl acetate, 2.0mg/L6- benzyl aminoadenine is added in every liter of culture medium；More
The component of injured tissue proliferated culture medium are as follows: using MS as minimal medium, 0.05mg/L methyl α-naphthyl acetate is added in every liter of culture medium,
2.0mg/L Thidiazuron.
8. the beautiful fan that any one of -7 method obtains according to claim 1.
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| CN201910300804.6A CN109997696A (en) | 2019-04-15 | 2019-04-15 | The rapid propagation method of succulent jade fan |
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| CN201910300804.6A CN109997696A (en) | 2019-04-15 | 2019-04-15 | The rapid propagation method of succulent jade fan |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111149647A (en) * | 2020-02-25 | 2020-05-15 | 闽卉（福建）园艺有限公司 | Planting method of Dahe brocade |
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| US20020144303A1 (en) * | 2001-01-16 | 2002-10-03 | Burns John Austin | Novel multiple shoot proliferation and regeneration system for plants |
| CN106305417A (en) * | 2016-08-19 | 2017-01-11 | 天津市园林花卉示范中心 | Haworthia truncata ex-vitro rooting tissue culture seedling growing method |
| CN106857256A (en) * | 2017-02-21 | 2017-06-20 | 淮北师范大学 | The method that beautiful dew breeding potential is improved based on callus induction Regeneration Ways |
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| US20020144303A1 (en) * | 2001-01-16 | 2002-10-03 | Burns John Austin | Novel multiple shoot proliferation and regeneration system for plants |
| CN106305417A (en) * | 2016-08-19 | 2017-01-11 | 天津市园林花卉示范中心 | Haworthia truncata ex-vitro rooting tissue culture seedling growing method |
| CN106857256A (en) * | 2017-02-21 | 2017-06-20 | 淮北师范大学 | The method that beautiful dew breeding potential is improved based on callus induction Regeneration Ways |
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| CN111149647A (en) * | 2020-02-25 | 2020-05-15 | 闽卉（福建）园艺有限公司 | Planting method of Dahe brocade |
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