CN109984859A - Small Animal Anesthesia Test Chamber with Fixtures - Google Patents

Abstract

The invention discloses a small animal anesthesia test box with a fixing device, comprising an anesthesia box, an air-oxygen mixed gas tank and an anesthetic volatilization tank. An anesthesia port is opened on the side wall of the anesthesia box, and a positioning tube and a fixing table are arranged on the anesthesia port. The positioning pipe includes a bearing bottom plate and a plurality of surrounding plates, the bearing bottom plate is provided with a fixing opening, the two side walls of the bearing bottom plate are respectively the first side wall and the second side wall, and the surrounding plate is hinged with the adjacent surrounding plate to form an arc-shaped cover, One side of the arc-shaped cover is hinged with the first side wall, and the other side is fixedly connected with the second side wall to form a positioning tube. There is a push rod, the end of the push rod is provided with a push plate, and the push plate is matched with the positioning pipe in clearance. The structure is simple, can effectively anesthetize small animals, and at the same time, the animal body can be exposed outside the anesthesia box, which is convenient for the experimenter to perform stress experiments on the experimental target.

Description

Small Animal Anesthesia Test Chamber with Fixtures

technical field

The invention relates to the technical field of animal experiments, in particular to a fixing device for inhalation anesthesia experiments of small animals.

Background technique

In experiments that study the effects of long-term (>0.5h) exposure to inhaled anesthetics on rats/mice, the issue of exposure devices must be involved. The animals were kept on spontaneous breathing and mechanical ventilation was avoided by intubation or tracheotomy. At present, the animal is mainly anesthetized by placing the small animal directly in a transparent and airtight anesthesia box, and then injecting anesthesia gas to anesthetize the animal. This anesthesia method can well ensure that the animal is anesthetized by spontaneous breathing, but it is difficult for the experimenter to effectively. Verifying whether the anesthesia is completed, the degree of anesthesia is too deep, it is also easy to lead to inaccurate experimental data, and even lead to the direct death of the animal, the closed anesthesia box is also difficult for the user to detect the stress response of the animal during the anesthesia process, and the box needs to be opened. This kind of detection method is very cumbersome, the anesthesia is not in place, and the box needs to be closed again, and then the anesthetic gas is introduced to ensure the concentration of the anesthetic agent, and opening the box for detection will also leak the anesthetic gas in the box. , which may affect the experimenter and affect the efficiency of the experiment. By placing a part of a small animal, such as the head, into the anesthesia room for anesthesia, the animal usually struggles violently. In order to avoid the struggle, the limbs of the animal need to be immobilized, but the immobilization operation is also very cumbersome, which reduces the experimental efficiency and cannot guarantee that the small animal can Good anesthesia.

SUMMARY OF THE INVENTION

In view of the deficiencies of the prior art, the present invention provides a small animal anesthesia test box with a fixing device, which has a simple structure, can effectively anesthetize small animals, and at the same time, the animal body can be exposed outside the anesthesia box, which is convenient for the experimenter to understand the experimental target. Conduct stress experiments.

The invention provides a small animal anesthesia test box with a fixing device, comprising an anesthesia box, an air-oxygen mixed gas tank and an anesthetic vaporizer, wherein the air-oxygen mixed gas tank and the anesthesia vaporizer are respectively connected to the anesthesia box through an air guide tube. , the side wall of the anesthesia box is provided with an anesthesia port, the anesthesia port is provided with a positioning tube and a fixing table for fixing the animal head, the positioning tube includes a bearing bottom plate and a number of continuously arranged surrounding plates, the bearing The bottom plate is provided with four fixing openings for fixing the limbs of the target object, the two side walls of the carrying bottom plate are the first side wall and the second side wall respectively, the enclosing plate is hingedly arranged with the adjacent enclosing plate, and the enclosing plate is hinged. The plates are provided with contact planes at the hinge joints, and the contact planes of the adjacent surrounding plates interfere with each other to form an arc-shaped cover. The wall is fixed and connected to form a positioning tube, one end of the positioning tube is detachably connected with the anesthesia port, and the other end is provided with a push port, the push port is inserted with a push rod, and the end of the push rod is provided with a push plate, so The push plate and the positioning tube are in clearance fit to form a sliding connection between the push rod and the positioning tube.

The beneficial effect of this arrangement is: using the above scheme, after the arc-shaped cover and the bearing base plate are combined to form a positioning tube, the positioning tube is fixedly connected to the anesthesia box. The enclosing plate is fixed by the insertion of the bearing bottom plate and the box of the anesthesia box, and the length of the arc cover composed of the enclosing plate is smaller than that of the bearing plate, which will not affect the installation. Push the test object through the push rod to gradually approach the anesthesia box, and keep its head in the anesthesia box. There is anesthesia gas in the anesthesia box. Here, as the preferred positioning tube, a transparent material is used, which is convenient to directly observe the object where the experimental object is located. After a period of time, the arc-shaped cover can be opened to expose the experimental object, which is convenient for the experimenter to conduct stress experiments on mice. operation to assess the anesthesia state of the experimental object, and at the same time after the completion of sufficient anesthesia, the experimental object can be directly tested on the carrying base plate. The leakage of anesthetic gas can effectively anesthetize small animals, and at the same time, the animal body can be exposed outside the anesthesia box, which is convenient for the experimenter to conduct stress experiments on the experimental target and conduct subsequent experiments.

In the present invention, the fixing table is further arranged between the anesthesia port and the positioning tube and is fixedly connected with the anesthesia box, the fixing table is provided with an insertion port, and the bearing base plate and the insertion port are inserted and matched to form the positioning tube and the insertion port. The fixed connection of the fixing table, the fixing table is provided with perforations to form the communication between the positioning tube and the anesthesia port, the inner wall of the fixing table is provided with a conical elastic retaining ring, and the tip of the elastic retaining ring is arranged toward the anesthesia port, The tip of the elastic retaining ring is provided with a cross-shaped cutout.

The beneficial effect of this arrangement is: adopting the above scheme, setting a fixed table, and setting a conical spring retaining ring, the tip of the retaining ring is provided with a cross-shaped incision, which is convenient for the head of the experimental target to protrude, and at the same time, the retaining ring can play a certain position. Function, after the head of the target protrudes out, it forms a limit on the position of the neck of the target to prevent the target from retracting the head. The angle of the tapered tip here is relatively large. It is found that this structure is simple and convenient for implementation.

In the present invention, a soft sealing ring is further arranged on the inner wall of one end of the fixing platform close to the positioning pipe, and the sealing ring is densely covered with soft fluff.

The beneficial effects of this setting are: adopting the above scheme, setting a sealing ring to assist in positioning the target, and at the same time setting soft fluff to prevent trauma and scratching of the target, and can effectively reduce the overflow of anesthetic gas in the anesthesia box, which is not conducive to the experiment. influencers.

In the present invention, the bearing base plate is further provided with sliding grooves along the length direction on both sides of the fixing opening, and guiding ribs are arranged on the inner wall of the fixing opening along the length direction of the bearing base plate, and the guiding ridges extend to slide on both sides. Two sliding blocks are slidably arranged in the sliding groove, guide grooves are arranged on the side walls of the sliding blocks, and the guiding ribs are slidingly matched with the guiding grooves to form a sliding block in the sliding groove. The opposite side walls of the two sliding blocks are provided with arc-shaped notches, and the arc-shaped notches on the two sliding blocks fit together to form a limit opening for limiting the limbs of animals.

The beneficial effects of this arrangement are: using the above solution, the positioning method for the limbs of the target object is simple. When the target enters the positioning tube, the fixed port is first closed with a sliding block, so as to prevent the limbs of the target from falling from the fixed port during the traveling process. After the target reaches the designated position, slide the sliding block to expose the fixed opening. The fixed opening here is set larger to ensure that the limbs of the target can fall into the corresponding fixed opening, and then slide the corresponding fixed opening by sliding on both sides of the opening. The arc-shaped notches on the sliding block are combined to form a limit opening, which forms the locking of the target limbs, so as to realize the restraint of the target limbs. Slide along the chute to fit target animals of different sizes and improve the fit performance.

The present invention is further provided that extending grooves are extended on the inner wall of the arc-shaped notch toward the four corner edges of the bearing bottom plate, and positioning posts are provided at the four corner edges of the bearing bottom plate.

The beneficial effect of this arrangement is that: by adopting the above scheme, a positioning column can be set up, and a thin rope can be tied on the positioning column, and the limbs of the target animal in the limit opening can be tied and fixed with the positioning column through the thin rope to realize positioning, and at the same time setting There are extension slots, which are convenient to stretch and stretch the limbs of the target animal and fully locate.

In the present invention, an insertion slot and a fixing rod are further arranged between the two sliding blocks, and the fixing rod and the insertion slot are fitted with interference to form a fixed connection between the two sliding blocks.

The beneficial effect of this arrangement is: by adopting the above solution, the two sliding blocks are fixed together after being inserted and matched, so as to achieve a better positioning effect.

According to the present invention, at least two positioning protrusions are provided on the second side wall of the carrying base plate, a card interface is formed between adjacent positioning protrusions, and fixing holes are coaxially provided on the positioning protrusions. The connecting end of the arc-shaped cover and the second side wall is provided with an inserting block, the inserting block is clamped with the card interface, a locking hole is arranged on the inserting block, and a positioning pin is also arranged on the bearing bottom plate, and the positioning The pin passes through the fixing hole and the locking hole to form a fixed connection between the arc cover and the bearing bottom plate.

The beneficial effects of this arrangement are: using the above solution, the arc-shaped cover is connected to the second side wall in this way, the structure is simple, the fixing and unlocking are very convenient, and the use effect of the device and the test efficiency are improved.

Description of drawings

1 is a schematic structural diagram of an embodiment of the present invention;

2 is a cross-sectional view of an anesthesia box in an embodiment of the present invention;

3 is an exploded view of an anesthesia box in the embodiment of the present invention;

4 is a schematic structural diagram of a positioning tube in an embodiment of the present invention;

FIG. 5 is a schematic structural diagram of a sliding block in an embodiment of the present invention.

Detailed ways

It can be seen from FIG. 1 to FIG. 3 that the present invention discloses a small animal anesthesia test box with a fixing device, including an anesthesia box 1, an air-oxygen mixed gas tank 2 and an anesthetic vaporizer 3. The air-oxygen mixed gas tank 2 and The anesthetic vaporizers 3 are respectively arranged in conduction with the anesthesia box 1 through the air guide tube, an anesthesia port is opened on the side wall of the anesthesia box 1, and the anesthesia port is provided with a positioning tube 4 and a fixing table 5 for fixing the animal head, The positioning tube 4 includes a bearing bottom plate 41 and a plurality of continuously arranged surrounding plates 42. The bearing bottom plate 41 is provided with four fixing openings for fixing the limbs of the target object. The two side walls of the bearing bottom plate 41 are the first The side wall and the second side wall, the enclosing plate 42 is hingedly arranged with the adjacent enclosing plate 42, the enclosing plate 42 is provided with a contact plane at the hinge, and the abutting planes of the adjacent enclosing plates 42 collide with each other and combine to form an arc-shaped cover One side of the arc-shaped cover is hinged with the first side wall of the bearing bottom plate 41, and the other side is fixedly connected with the second side wall of the bearing bottom plate 41 to form a positioning tube 4. One end of the positioning tube 4 is detachable from the anesthesia port. Connected, the other end is provided with a push port, the push port is inserted with a push rod 6, and the end of the push rod 6 is provided with a push plate 61, the push plate 61 and the positioning tube 4 are gap-fitted to form the push rod 6 and the positioning Sliding connection of tube 4. With the above solution, after the arc-shaped cover and the bearing base plate 41 are combined to form the positioning tube 4, the positioning tube 4 is fixedly connected to the anesthesia box 1. Here, the fixed connection can be made by setting the length of the bearing base plate 41 to be longer than the surrounding plate. 42. The bearing bottom plate 41 is inserted into the anesthesia box 1 to form a fixation, and the length of the arc cover formed by the surrounding plate 42 is smaller than that of the bearing plate, which will not affect the installation. 4. Then push the test object through the push rod 6 to make it gradually approach the anesthesia box 1, and keep its head in the anesthesia box 1. There is anesthesia gas in the anesthesia box 1, and keep pushing the object. After 30 seconds, the test object is basically It is already under mild anesthesia. Here, as the preferred positioning tube 4, a transparent material is used, which is convenient to directly observe the object where the experimental object is located. After a period of time, the arc-shaped cover can be opened to expose the experimental object, which is convenient for the experiment. The experimenter conducts stress experiments on the mice to evaluate the anesthesia state of the experimental subjects. At the same time, after the sufficient anesthesia is completed, the experimental subjects can be directly subjected to subsequent tests on the carrying base plate 41. This simple structure is convenient for operation and realizes anesthesia effect. Well, there is no need to open the anesthesia box 1 to avoid the leakage of anesthetic gas, which can effectively anesthetize small animals, and at the same time, the animal body can be exposed outside the anesthesia box 1, which is convenient for the experimenter to perform stress experiments on the experimental target and conduct follow-up experiments.

The above-mentioned fixing table 5 is arranged between the anesthesia port and the positioning tube 4 and is fixedly connected with the anesthesia box 1. The fixing table 5 is provided with a plug-in port 53, and the bearing bottom plate 41 and the plug-in port 53 are plugged and matched to form a positioning pipe. 4. The fixed connection with the fixed table 5. The fixed table 5 is provided with perforations to form the communication between the positioning tube 4 and the anesthesia port. The inner wall of the fixed table 5 is provided with a tapered elastic retaining ring 51. The tip of the ring 51 is set toward the anesthesia port, and the tip of the elastic retaining ring 51 is set with a cross-shaped cutout 52 . With the above solution, the fixing table 5 is set, and the conical retaining ring 51 is set, and the tip of the retaining ring is provided with a cross-shaped cut 52, which is convenient for the head of the experimental target to protrude. At the same time, the retaining ring 51 can play a certain role in positioning. After the head of the target protrudes out, it forms a limit on the position of the neck of the target to prevent the target from retracting its head. The angle of the tapered tip here is relatively large, and it can be set to 145° as a preferred option to prevent the head of the target from being unable to protrude. Simple structure is convenient for realization.

A soft sealing ring is arranged on the inner wall of one end of the above-mentioned fixing table 5 close to the positioning tube 4 , and the sealing ring is densely covered with soft fluff. Using the above scheme, a sealing ring is provided to assist in positioning the target object, and soft fluff is provided at the same time to prevent trauma and scratching of the target object, and can effectively reduce the overflow of anesthetic gas in the anesthesia box 1, which has an impact on the experimenter.

The above-mentioned bearing base plate 41 is provided with chute 44 along the length direction on both sides of the fixing opening, and the inner wall of the fixing opening is provided with guiding ribs along the length direction of the bearing base plate 41, and the guiding ribs extend into the sliding grooves 44 on both sides. Two sliding blocks 45 are slidably arranged in the sliding groove 44 , a guiding groove 452 is provided on the side wall of the sliding block 45 , and the guiding ribs and the guiding grooves 452 are slidingly matched to form the sliding block 45 When sliding in the chute 44, the opposite side walls of the two sliding blocks 45 are provided with arc-shaped notches 451, and the arc-shaped notches 451 on the two sliding blocks 45 fit together to form a limit for limiting animal limbs mouth. With the above solution, the positioning method for the limbs of the target is simple. When the target enters the positioning tube 4, the fixed opening is first closed by the sliding block 45, so as to prevent the limbs of the target from falling out of the fixed opening during the traveling process. After setting the position, slide the sliding blocks 45 to expose the fixed openings. The fixed openings here are set larger to ensure that the target limbs can fall into the corresponding fixed openings, and then slide the sliding blocks 45 on both sides of the corresponding fixed openings. The arc-shaped notches 451 on the shifting block 45 are combined to form a limit opening, which forms the locking of the target limbs, so as to realize the restraint of the target limbs. Slide along the chute 44 to adapt to target animals of different sizes and improve the adaptability.

Extending grooves 454 are formed on the inner wall of the above-mentioned arc-shaped notch 451 toward the four corner edges of the bearing bottom plate 41 , and positioning posts 46 are provided at the four corner edges of the bearing bottom plate 41 . By adopting the above scheme, the positioning post 46 is arranged, and a thin rope can be tied on the positioning post 46, and the limbs of the target animal in the limit opening are tied and fixed with the positioning post 46 through the thin rope to realize positioning, and an extension groove 454 is provided at the same time. , it is convenient to stretch and stretch the limbs of the target animal and fully locate.

An insertion slot 453 and a fixing rod are arranged between the two sliding blocks 45 , and the fixing rod and the insertion slot 453 are interference fit to form a fixed connection between the two sliding blocks 45 . The two sliding blocks 45 are inserted and fitted together and then fixed together to achieve a better positioning effect.

At least two positioning protrusions are provided on the second side wall of the above-mentioned bearing bottom plate 41, and a card interface is formed between adjacent positioning protrusions. The connecting end of the second side wall is provided with an insert block, the insert block is clamped with the card interface, the insert block is provided with a locking hole, and the bearing bottom plate 41 is also provided with a positioning pin 43 , and the positioning pin 43 penetrates An arc-shaped cover is fixedly connected to the carrying base plate 41 through the fixing hole and the locking hole. The arc-shaped cover is connected to the second side wall in this way, the structure is simple, the fixing and unlocking are very convenient, and the use effect of the equipment and the test efficiency are improved.

The above-mentioned embodiments are only preferred embodiments of the present invention, and cannot be used to limit the scope of rights of the present invention. Therefore, equivalent changes made according to the scope of the patent application of the present invention, such as equivalent products using similar processes and similar structures, still belong to the scope of the present invention. Scope covered by the present invention.

Claims (7)

1. a small animal anesthesia test box with fixture, comprising anesthesia box, air-oxygen mixed gas tank and anesthesia volatilizer, described air-oxygen mixed gas tank and anesthesia volatilizer are respectively set through air conduit and anesthesia box conduction, It is characterized in that: an anesthesia port is opened on the side wall of the anesthesia box, a positioning tube and a fixing table for fixing the animal head are arranged on the anesthesia port, and the positioning tube includes a bearing bottom plate and a number of continuously arranged surrounding plates, The carrying base plate is provided with four fixing openings for fixing the limbs of the target object, the two side walls of the carrying base plate are respectively a first side wall and a second side wall, and the enclosing board is hingedly arranged with the adjacent enclosing board, The surrounding plates are provided with contact planes at the hinged joints, and the contact planes of adjacent surrounding plates interfere with each other to form an arc-shaped cover. The second side wall is fixedly connected and combined to form a positioning tube. One end of the positioning tube is connected to the detachable anesthesia port, and the other end is provided with a push port. The push port is inserted with a push rod, and the end of the push rod is provided with a push rod. The push plate and the positioning tube are in clearance fit to form a sliding connection between the push rod and the positioning tube. 2. The small animal anesthesia test box with fixing device according to claim 1, is characterized in that: described fixing table is arranged between anesthesia port and positioning tube and is fixedly connected with anesthesia box, and described fixing table is provided with an insertion port, the bearing bottom plate and the insertion port are inserted and matched to form a fixed connection between the positioning tube and the fixing table, the fixing table is provided with perforations to form the communication between the positioning tube and the anesthesia port, and the inner wall of the fixing table is provided with a tapered The elastic retaining ring is arranged in a shape, the tip of the elastic retaining ring is arranged towards the anesthesia port, and the tip of the elastic retaining ring is provided with a cross-shaped incision. 3. The small animal anesthesia test box with a fixing device according to claim 2, is characterized in that: a soft sealing ring is arranged on the inner wall of one end of the fixing table close to the positioning tube, and the sealing ring is densely covered with soft fluff . 4. The small animal anesthesia test box with fixing device according to claim 1 or 2 or 3, wherein the bearing bottom plate is provided with chute along the length direction on both sides of the fixing opening, and the inner wall of the fixing opening is provided with a chute along the length direction. The upper guide ribs are arranged along the length direction of the bearing bottom plate, and the guide ribs extend to the two sides of the chute and are provided with two sliding blocks, and the side walls of the sliding blocks are provided with two sliding blocks. Guide grooves, the guide ribs and the guide grooves slidingly cooperate to form the sliding blocks of the sliding blocks in the sliding grooves. The opposite side walls of the two sliding blocks are provided with arc-shaped gaps, and the two sliding blocks are arc-shaped. The notch is fitted to form a limit opening for limiting the limb of the animal. 5. The small animal anesthesia test box with a fixing device according to claim 4, characterized in that: extending grooves are arranged on the inner wall of the arc-shaped notch toward the four corner edges of the bearing bottom plate, and the four corner edges of the bearing bottom plate are provided with Positioning post. 6. The small animal anesthesia test box with a fixing device according to claim 4, wherein the two sliding blocks are provided with an insertion slot and a fixing rod, and the fixing rod and the insertion slot are formed by interference fit. Fixed connection between two sliders. 7. The small animal anesthesia test box with a fixing device according to claim 1, characterized in that: at least two positioning protrusions are provided on the second side wall of the bearing bottom plate, and adjacent positioning protrusions are formed with at least two positioning protrusions. a card interface, a fixing hole is coaxially provided on the positioning protrusion, an insertion block is arranged at the connecting end of the arc-shaped cover and the second side wall, the insertion block is clamped with the card interface, and an insertion block is arranged on the insertion block A locking hole is provided, and a positioning pin is also provided on the bearing base plate, and the positioning pin passes through the fixing hole and the locking hole to form an arc-shaped cover and is fixedly connected to the bearing base plate.