CN109984731A - Real-time angioradiographic system and method based on whole audience time domain OCT technology - Google Patents
Real-time angioradiographic system and method based on whole audience time domain OCT technology Download PDFInfo
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B3/00—Apparatus for testing the eyes; Instruments for examining the eyes
- A61B3/10—Objective types, i.e. instruments for examining the eyes independent of the patients' perceptions or reactions
- A61B3/12—Objective types, i.e. instruments for examining the eyes independent of the patients' perceptions or reactions for looking at the eye fundus, e.g. ophthalmoscopes
- A61B3/1225—Objective types, i.e. instruments for examining the eyes independent of the patients' perceptions or reactions for looking at the eye fundus, e.g. ophthalmoscopes using coherent radiation
- A61B3/1233—Objective types, i.e. instruments for examining the eyes independent of the patients' perceptions or reactions for looking at the eye fundus, e.g. ophthalmoscopes using coherent radiation for measuring blood flow, e.g. at the retina
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/0059—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence
- A61B5/0062—Arrangements for scanning
- A61B5/0066—Optical coherence imaging
Abstract
The invention discloses a kind of real-time angioradiographic system and method based on whole audience time domain OCT technology, including light source, collimating mirror, spectroscope, microcobjective, the first motorized precision translation stage, reference mirror, actuator, the second motorized precision translation stage, two-dimensional camera, computer, multi-channel signals generator and image pick-up card.The present invention directly acquires the vascular distribution in cross section using whole audience time domain OCT technology as a result, avoiding complicated pilot process and thus caused motion artifact；Other than it can be carried out conventional blood vessel structure radiography, moreover it is possible to dynamic arthrography is carried out for certain layer in sample of vascular system, to obtain the dynamic information of vascular system.The present invention is a kind of real-time Angiography, real-time regulation system parameter and change can observe object etc. as needed；The fine vasculars distributed intelligence such as capillary and blood vessel end can be obtained；Also have many advantages, such as that result is accurate, system structure is simple, control and data processing are simple, requirement on devices is low and low in cost.
Description
Technical field
The present invention relates to the Angiography fields using optical means, more particularly to one kind to be based on whole audience time domain OCT
The real-time angioradiographic system and method for technology.
Background technique
Many lesions have performance on blood vessel, such as: cancer, diabetes, psoriasis, the nervous system disease, wound and inflammation
Disease etc..The variation for understanding blood vessel is of great significance to probing into the cause of disease and developing treatment means.It is made using more modern medicine
Shadow art mainly has based on technologies such as ultrasonic wave, nuclear magnetic resonance and confocal microscopes.The harm of ultrasonic technology is small and penetrates depth, but
Resolution ratio is low, need to especially contact by couplant with target, this can make troubles even not applicable in some cases.Nuclear-magnetism
Resonance technique has the characteristics that multi-parameter, stage construction and multi-faceted imaging, it just can show blood vessel without contrast agent, to many diseases
The positioning of change, qualitative and quantitative Diagnosis have a clear superiority, but its image resolution ratio is lower.Confocal microscope technology can get pole
High lateral resolution, but image taking speed is slow low with the resolution ratio along depth direction.
In addition, the mode of infection is clinically also widely used to vascular system radiography, such methods can get higher resolution
Rate, but contrast agent need to be injected, this can bring uncomfortable or damage to human body.By taking Fundus angiography as an example, fundus fluorescence blood vessel is made
Shadow art (Fundus fluorescein angiography) and Indocyanine-Green art (Indocyanine green
Angiography) it is usual way, is respectively used to observation retina and choroidal vascular distribution, the blood-vessel image of acquisition are clear
It is clear as it can be seen that but there is following problems: 1) dyestuff need to be injected intravenously, the operation is time-consuming and is possible to that human body can be brought centainly
Damage or cause discomfort；2) dyestuff, which can be leaked and be dyed to surrounding tissue, itself can make capillary fall off or new green blood
The boundary that pipe is formed fogs, and is unfavorable for observing；3) depth information provided is less, i.e. longitudinal capacity of decomposition is weak, it is difficult to observe
The vascular distribution of certain layer.
Newly-developed get up based on optical coherent chromatographic imaging (Optical coherence tomography, OCT)
The angiography of technology, also referred to as optical coherence tomography angiography (OCT angiography, OCTA), because without note
Penetrate dyestuff, high vertical resolution, high s/n ratio, high sensitivity and it is quick the features such as, gradually medical basic research and facing
It plays a significant role in bed diagnosis.OCT technology is generally divided into two class of time domain and frequency domain.As shown in left in Figure 2, frequency domain OCT
Technology is not necessarily to the mechanical scanning along z-axis, but obtains depth side by carrying out Fourier transformation to collected interference signal
To all information, the A-scan information i.e. in figure；In addition the transversely scanning of y-axis, can obtain the two dimension in the Y-Z of longitudinal section
Information, i.e. B-scan information；In conjunction with the scanning of transversely x-axis, the three-dimensional information of sample can be obtained.Mechanical scanning is to restrict
One of the principal element of OCT image speed, whole letters in frequency domain OCT technology sampling depth direction without axial scan and simultaneously
Breath, therefore compared to time domain OCT technology, there is faster image taking speed and higher signal-to-noise ratio etc., therefore study and answer at present
With it is more be frequency domain OCT technology, existing OCTA is also all based on frequency domain OCT technology.
Such as Carlo et al. document (T E de Carlo, et al.Advances in optical coherence
Tomography angiography.US Ophthalmic Review, 2016,9 (1): 37-40.) in people as shown in Figure 1
Eyes retina and choroidal artery visualisation, it is known that: the blood vessel mainly network-like distribution in the X-Y of cross section, and cut vertical
Distribution in the Y-Z of face is usually discontinuous and fails to be formed network-like.Therefore, to the studies and clinical application of vascular system, mainly
It is carried out in X-Y plane.Based on the angiography of frequency domain OCT technology, as shown in left in Figure 2, workflow is usual are as follows: one
Tie up two dimension-in two dimension-three-dimensional-motion artifact correction in the Y-Z of (Z axis)-longitudinal section-number chromatography slice-cross section X-Y
Radiography data processing-vascular distribution.The technology is suitable for three-dimensional angiography, but there is following deficiencies: 1) signal acquisition
It is big with data processing complex, data volume, it need to could obtain through the pilot process such as three-dimensional reconstruction and number chromatography slice as a result, leading
Cause cannot observe result, thus cannot real-time regulation system parameter as needed and selection object observing etc. in real time；2) in signal
In collection process, environmental perturbation or the mobile caused motion artifact of sample easily occurs, the illusion is in the image procossing in later period
It is difficult to be completely eliminated, therefore adverse effect can be brought to result；3) to guarantee that have sufficiently long depth of focus to be seen right to cover
As that can only carry out low numerical aperture NA imaging, cause lateral resolution δ x low and be difficult to observe tiny blood vessels, such as blood vessel end
With capillary etc.；4) due to not being real-time shadowgraph technique, so that the dynamic that cannot be changed over time to same position is made
Shadow observation, and circulation is adjusted the dynamic information of blood vessel and pathological changes diagnosis is also of great significance.
Time domain OCT technology needs axial scan, in which: lateral point by point scanning imaging mode, because image taking speed is slow and signal-to-noise ratio
Low disadvantage, at present less use.And whole audience time domain OCT technology is that parallel detecting mode is not necessarily to as shown in right in Figure 2
Transversal scanning can acquire the two-dimensional signal in the X-Y of cross section simultaneously, with image taking speed is fast, signal-to-noise ratio is relatively high, system
Control and data processing are simple, high NA can be used carries out high δ x imaging, implementable dynamic is focused and requirement on devices is low and cost
The advantages that cheap, therefore obtain extensive concern and application.Whole audience time domain OCT technology directly obtains cross section without scanning
Two-dimensional signal in X-Y makes it more suitable for angiography, and the combination of the two can be achieved the real-time angiography of high-resolution and see
It examines, but yet there are no the report of the angiography based on whole audience time domain OCT technology.
Summary of the invention
The technical problem to be solved by the present invention is overcoming the deficiencies of the prior art and provide a kind of based on whole audience time domain OCT skill
The Real-time High Resolution rate angioradiographic system and method for art, come directly acquire vascular distribution in sample in cross section as a result, with
Avoid complicated pilot process and thus caused motion artifact.The present invention is in addition to providing conventional blood vessel structure radiography function
Outside, moreover it is possible to dynamic arthrography observation is carried out for certain layer in sample of vascular system, to obtain the dynamic information of vascular system.
The technical solution adopted by the present invention to solve the technical problems is: the real-time blood vessel based on whole audience time domain OCT technology
Angiography system, including light source, collimating mirror, spectroscope, microcobjective, the first motorized precision translation stage, sample stage, the first lens, reference
Mirror, actuator, the second motorized precision translation stage, the second lens, two-dimensional camera, computer, multi-channel signals generator and image pick-up card；
Wherein,
The light beam that light source issues, is collimated incident spectroscope after mirror collimation, the mirror that is split be divided into reflection sample beam and
The reference beam of transmission；Sample beam is focused in sample by microcobjective, and microcobjective is mounted on the first motorized precision translation stage,
Sample is placed on sample stage；By the first lens focus on reference mirror, reference mirror is fixed on actuator reference beam, and first
Lens and actuator are mounted on the second motorized precision translation stage；
By sample retroreflection or scattering after, along backtracking to spectroscopical sample beam, through spectroscopical part
Into end of probe；Be referenced mirror reflection after, along backtracking to light splitting reference beam, the mirror that is split reflection part enter spy
Survey end；Into the sample beam and reference beam of end of probe, by the second lens focus in two-dimensional camera；
It is electronic flat that computer control multi-channel signals generator generation controls the first motorized precision translation stage, actuator and second respectively
The driving signal of moving stage；By the axial movement of the second motorized precision translation stage, to match the light between sample beam and reference beam
The depth location that sample is imaged in journey and selection；Actuator makees the operation such as phase shift or Carrier Modulation with reference mirror；First electricity
Dynamic translation stage is moved axially with microcobjective, carries out dynamic focusing to sample；Computer passes through Image Acquisition card control two
It ties up camera and acquires interference signal, the collected interference signal of two-dimensional camera passes through image pick-up card again and is transmitted at computer
Reason；Multi-channel signals generator exports synchronizing clock signals simultaneously, goes the data acquisition of control image pick-up card, realizes that first is electronic
Synchronously control between translation stage, actuator, the second motorized precision translation stage and two-dimensional camera.
The light source is non-coherent broad band light source, such as white light source；It can also be Coherent Wideband light source, such as broad band laser light
Source, but eliminating coherence processing need to be carried out, existing cross-interference issue is imaged to eliminate whole audience time domain OCT.
The spectroscope is the broadband spectroscope that energy splitting ratio is 50:50.
First motorized precision translation stage is used to carry out dynamic focusing to sample；To keep in dynamic focussing process, light splitting
For mirror to sample focal plane and to the equivalent optical path of reference mirror, the first motorized precision translation stage and the second motorized precision translation stage need synchronization action, simultaneously
Meet relationship: nair△lr=nsam△lf, in which: △ lrWith △ lfThe respectively axial internal clearance of reference mirror and focal plane, nairWith
nsamThe respectively refractive index of air and sample.
Based on the real-time angiographic method of whole audience time domain OCT technology, including blood vessel dynamic arthrography and blood vessel structure radiography
Two methods；
Blood vessel dynamic arthrography method, comprising the following steps:
Step 11: activation system carries out sampling time interval parameter setting；
Step 12: by the axial movement of the first motorized precision translation stage and the second motorized precision translation stage, to select that sample is imaged
Depth location；Hereafter the second motorized precision translation stage remains stationary, and actuator makees phase shift with reference mirror or Carrier Modulation operates, together
When be spaced certain layer of interference signal in acquisition sample according to set time by two-dimensional camera；
Step 13: to the interference signal of two-dimensional camera acquisition, at phase shift or Carrier Modulation signal extracting algorithm
Reason specifically includes amplitude and phase information to obtain certain layer of field distribution information changed over time in sample；
Step 14: to the field distribution information changed over time, using radiography data processing method, to obtain certain in sample
The multidate information that the vascular system of layer changes over time；
Step 15: generating and show the dynamic image of vascular system；
Step 16: repeating step 12 to step 15, the first motorized precision translation stage and the axial movement of the second motorized precision translation stage, selection
Other layers of progress blood vessel dynamic arthrography imaging in sample；Or terminate program.
Blood vessel structure angiographic method, comprising the following steps:
Step 21: activation system carries out imaging depth range, sampling interval and scanning speed parameter setting；
Step 22: the axially consecutive scanning of the second motorized precision translation stage, the first motorized precision translation stage carry out dynamic focusing, actuator band
Reference mirror make phase shift or Carrier Modulation operation, two-dimensional camera acquire interference signal in sample simultaneously；
Step 23: to the interference signal of two-dimensional camera acquisition, at phase shift or Carrier Modulation signal extracting algorithm
Reason, to obtain the field distribution information of different layers in sample, specifically includes amplitude and phase information；
Step 24: to the field distribution information of adjacent layer in sample, using radiography data processing method, to obtain in sample
The structural information of vascular system；
Step 25: generating and show the structural images of vascular system；
Step 26: change imaging parameters repeat step 22 to step 25, re-start blood vessel structure contrast imaging；Or knot
Shu Chengxu.
Compared with the prior art, the invention has the advantages that:
1) present invention is a kind of real-time Angiography, can real-time regulation system parameter and change observation pair as needed
As etc..The existing angiography based on frequency domain OCT technology, imaging process and data processing are complex, need to pass through in some
Between process could obtain as a result, therefore cannot in real time radiography observation.Blood vessel proposed by the present invention based on whole audience time domain OCT technology
Radiography can carry out real-time radiography observation to the random layer in sample, be more suitable for medical diagnosis.
2) present invention can provide two kinds of operating modes: make to the structure between the dynamic arthrography and adjacent position of same position
Shadow.The existing angiography based on frequency domain OCT technology can only provide the structure visualisation of blood vessel.The present invention is in addition to structure
Outside radiography information, moreover it is possible to provide the multidate information of blood vessel, this mainly has benefited from the real-time of this technology.The dynamic information of blood vessel
Circulation is adjusted and pathological changes diagnosis is of great significance.
3) present invention can get the fine vascular distributed intelligence of capillary and blood vessel end etc..The whole audience that the present invention uses
Time domain OCT technology, can be used high NA to obtain high δ x, along with the high vertical resolution that OCT technology itself has, so as to
Obtain three-dimension high-resolution result.High NA existing short focus depth problem when being imaged, then solved, therefore have by dynamic focus technique
All layers in sample of three-dimension high-resolution microangiography result may be obtained.Frequency domain OCT technology not can be carried out then high NA at
Picture and implementation dynamic are focused.
4) result that the present invention obtains is more accurate, this is because the signal in same cross section is simultaneously acquired, avoids
The reason mutually jumped between different pixels points.In addition, the present invention also has simple system structure, control and data processing letter
The advantages that single and requirement on devices is low and low in cost.
Detailed description of the invention
Fig. 1 is optical fundus blood vessel system distribution example figure；
Fig. 2 is the operation principle schematic diagram of frequency domain OCT technology and whole audience time domain OCT technology；
Fig. 3 is schematic diagram of optical system of the invention；
Fig. 4 is control system schematic diagram of the invention；
Fig. 5 is flow chart of work methods of the invention.
In figure: 1. light sources；2. collimating mirror；3. spectroscope；4. microcobjective；5. the first motorized precision translation stage；6. sample；7. sample
Sample platform；8. the first lens；9. reference mirror；10. actuator；11. the second motorized precision translation stage；12. the second lens；13. two-dimensional camera；
14. computer；15. multi-channel signals generator；16. image pick-up card.
Specific embodiment
The present invention is further illustrated in the following with reference to the drawings and specific embodiments.
Real-time angioradiographic system proposed by the present invention based on whole audience time domain OCT technology, including optical system and control
System, it is as shown in Figure 3 and Figure 4 respectively.Include: light source 1, collimating mirror 2, spectroscope 3, microcobjective 4, the first motorized precision translation stage 5,
Sample 6, sample stage 7, the first lens 8, reference mirror 9, actuator 10, the second motorized precision translation stage 11, the second lens 12, two-dimensional camera
13, computer 14, multi-channel signals generator 15 and image pick-up card 16.
In optical system as shown in Figure 3, the light beam that light source 1 issues is collimated incident spectroscope 3, quilt after mirror 2 collimates
Spectroscope 3 is divided into the sample beam of reflection and the reference beam of transmission: sample beam is focused in sample 6 by microcobjective 4, shows
Speck mirror 4 is mounted on the first motorized precision translation stage 5, and sample 6 is placed on sample stage 7；Reference beam is focused on by the first lens 8
On reference mirror 9, reference mirror 9 is fixed on actuator 10, and the first lens 8 and actuator 10 are mounted on the second motorized precision translation stage 11
On.Light source 1 is non-coherent broad band light source, such as white light source；It can also be Coherent Wideband light source, such as broad band laser light source, but need to be into
Existing cross-interference issue is imaged to eliminate whole audience time domain OCT in the processing of row eliminating coherence.Spectroscope 3 is that energy splitting ratio is 50:50's
Broadband spectroscope.By 6 retroreflection of sample or scattering after, along backtracking to the sample beam of spectroscope 3, through spectroscope 3
Part enter end of probe；Be referenced mirror 9 reflect after, the reference beam along backtracking to spectroscope 3, the mirror 3 that is split reflection
Part enter end of probe.Into the sample beam and reference beam of end of probe, two-dimensional camera 13 is focused on by the second lens 12
On.
In control system as shown in Figure 4, computer 14 controls the generation of multi-channel signals generator 15 and controls first respectively
The driving signal of motorized precision translation stage 5, actuator 10 and the second motorized precision translation stage 11: it is moved by the axial direction of the second motorized precision translation stage 11
It is dynamic, to match the light path between sample beam and reference beam and select the depth location that sample 6 is imaged；Actuator 10
Make the operation such as phase shift or Carrier Modulation with reference mirror 9；First motorized precision translation stage 5 is moved axially with microcobjective 4, to sample
Product 6 carry out dynamic focusing.Computer 14 controls two-dimensional camera 13 by image pick-up card 16 and acquires interference signal, two-dimensional camera 13
Collected interference signal, which passes through image pick-up card 16 again and is transmitted to computer 14, to be handled.Multi-channel signals generator 15 is simultaneously
Synchronizing clock signals are exported, the data acquisition of control image pick-up card 16 is gone, realizes the first motorized precision translation stage 5, actuator 10, the
Synchronously control between two motorized precision translation stages 11 and two-dimensional camera 13.
Sample 6 is carried out in dynamic focussing process with microcobjective 4 in the first motorized precision translation stage 5, to keep spectroscope 3
It is overlapped to 6 focal plane of sample and to the relevant door of the equivalent optical path of reference mirror 9, i.e. holding with focal plane, the first motorized precision translation stage 5 and second
Motorized precision translation stage 11 needs synchronization action and meets relationship: nair△lr=nsam△lf, in which: △ lrWith △ lfRespectively reference mirror
Axial internal clearance, n with focal planeairAnd nsamThe respectively refractive index of air and sample.Relevant door is counter sample light in sample 6
Position when optical path difference is zero between beam and reference beam, relevant threshold range is determined by the coherence length of light source 1, up to micron
Magnitude, the only photon within relevant thresholding just participate in imaging.On the other hand, sample focal plane nearby differentiate by the lateral of image
Rate δ x highest.Therefore, it in dynamic focussing process, keeps relevant door to be overlapped with focal plane, can get optimal picture quality, help
In observation microcirculqtory system.
Real-time angiographic method proposed by the present invention based on whole audience time domain OCT technology, workflow such as Fig. 5 institute
Show, including two methods of blood vessel dynamic arthrography and blood vessel structure radiography.
Blood vessel dynamic arthrography method, comprising the following steps:
Step 11: activation system carries out the parameter settings such as sampling time interval；
Step 12: by the axial movement of the first motorized precision translation stage and the second motorized precision translation stage, to select that sample is imaged
Depth location；Hereafter the second motorized precision translation stage remains stationary, and actuator is made phase shift or Carrier Modulation etc. with reference mirror and operated,
It is spaced certain layer in acquisition sample of interference signal according to set time by two-dimensional camera simultaneously；
Step 13: to the interference signal of two-dimensional camera acquisition, being carried out using signal extracting algorithms such as phase shift or Carrier Modulations
Processing specifically includes amplitude and phase information to obtain certain layer of field distribution information changed over time in sample；
Step 14: to the field distribution information changed over time, using radiography data processing method, to obtain certain in sample
The multidate information that the vascular system of layer changes over time；
Step 15: generating and show the dynamic image of vascular system；
Step 16: repeating step 12 to step 15, the first motorized precision translation stage and the axial movement of the second motorized precision translation stage, selection
Other layers of progress blood vessel dynamic arthrography imaging in sample；Or terminate program.
Blood vessel structure angiographic method, comprising the following steps:
Step 21: activation system carries out the parameter settings such as imaging depth range, sampling interval and scanning speed；
Step 22: the axially consecutive scanning of the second motorized precision translation stage, the first motorized precision translation stage carry out dynamic focusing, actuator band
Reference mirror make the operation such as phase shift or Carrier Modulation, two-dimensional camera acquires interference signal in sample simultaneously；
Step 23: to the interference signal of two-dimensional camera acquisition, being carried out using signal extracting algorithms such as phase shift or Carrier Modulations
Processing, to obtain the field distribution information of different layers in sample, specifically includes amplitude and phase information；
Step 24: to the field distribution information of adjacent layer in sample, using radiography data processing method, to obtain in sample
The structural information of vascular system；
Step 25: generating and show the structural images of vascular system；
Step 26: change imaging parameters repeat step 22 to step 25, re-start blood vessel structure contrast imaging；Or knot
Shu Chengxu.
OCT signal extraction has the methods of a phase shift and Carrier Modulation, angiographic data processing have Doppler, speckle variance and
The methods of correlation map, these methods can be used by this patent.They are the routine techniques and method in respective field, therefore
Do not tire out herein and states.
The above is the one of the real-time angioradiographic system and method proposed by the present invention based on whole audience time domain OCT technology
As working condition.Due to human eye optical fundus blood vessel system be one of important circulatory system of human body and with eye and part it is systemic
Disease is closely related, therefore human eye Fundus angiography is that a main direction of studying and application field, the present invention can also be herein
It plays a role.When the present invention is used for human eye Fundus angiography, following change need to be made: microcobjective 4 be human eye dioptric system,
Sample 6 is human eye fundus tissue；No longer by the first motorized precision translation stage 5, but carried out by one Badal focus adjusting mechanism of setting
Dynamic is focused；No longer pass through the transverse shifting of sample stage 7, but guides by one fixation sighting target of setting, by it come to eyeground
Different transverse areas are imaged.These change and Badal dynamic focus adjusting mechanism and fixation sighting target etc., are ophthalmology imaging
The common technology in field, is not repeated herein.
Above-mentioned specific embodiment is used to illustrate the present invention, rather than limits the invention.Of the invention
In spirit and scope of protection of the claims, to any modifications and changes that the present invention makes, protection model of the invention is both fallen within
It encloses.
Claims (5)
1. the real-time angioradiographic system based on whole audience time domain OCT technology, it is characterised in that: including light source (1), collimating mirror (2),
Spectroscope (3), the first motorized precision translation stage (5), sample stage (7), the first lens (8), reference mirror (9), actuates microcobjective (4)
Device (10), the second motorized precision translation stage (11), the second lens (12), two-dimensional camera (13), computer (14), multi-channel signals generator
(15) and image pick-up card (16)；Wherein,
The light beam that light source (1) issues, is collimated incident spectroscope (3) after mirror (2) collimation, and the mirror that is split (3) is divided into the sample of reflection
The reference beam of product light beam and transmission；Sample beam is focused in sample (6) by microcobjective (4), and microcobjective (4) is mounted on
On first motorized precision translation stage (5), sample (6) is placed on sample stage (7)；Reference beam focuses on reference by the first lens (8)
On mirror (9), reference mirror (9) is fixed on actuator (10), and the first lens (8) and actuator (10) are mounted on the second electric translation
On platform (11)；
By sample (6) retroreflection or scattering after, the sample beam along backtracking to spectroscope (3), through spectroscope (3)
Part enters end of probe；Be referenced mirror (9) reflection after, the reference beam along backtracking to spectroscope (3), the mirror that is split (3)
The part of reflection enters end of probe；Into the sample beam and reference beam of end of probe, two dimension is focused on by the second lens (12)
On camera (13)；
Computer (14) control multi-channel signals generator (15) generates and controls the first motorized precision translation stage (5), actuator (10) respectively
With the driving signal of the second motorized precision translation stage (11)；By the axial movement of the second motorized precision translation stage (11), to match sample light
The depth location that sample (6) are imaged in light path and selection between beam and reference beam；Actuator (10) is with reference mirror
(9) make phase shift or Carrier Modulation operation；First motorized precision translation stage (5) is moved axially with microcobjective (4), to sample (6)
Carry out dynamic focusing；Computer (14) acquires interference signal, two-dimensional phase by image pick-up card (16) control two-dimensional camera (13)
Machine (13) collected interference signal, which passes through image pick-up card (16) again and is transmitted to computer (14), to be handled；Multiple signals hair
Raw device (15) export synchronizing clock signals simultaneously, go the data acquisition of control image pick-up card (16), realize the first electric translation
Synchronously control between platform (5), actuator (10), the second motorized precision translation stage (11) and two-dimensional camera (13).
2. the real-time angioradiographic system according to claim 1 based on whole audience time domain OCT technology, it is characterised in that: institute
The light source (1) stated is non-coherent broad band light source, such as white light source；It can also be Coherent Wideband light source, such as broad band laser light source, but need
Eliminating coherence processing is carried out, existing cross-interference issue is imaged to eliminate whole audience time domain OCT.
3. the real-time angioradiographic system according to claim 1 based on whole audience time domain OCT technology, it is characterised in that: institute
The spectroscope (3) stated is the broadband spectroscope that energy splitting ratio is 50:50.
4. the real-time angioradiographic system according to claim 1 based on whole audience time domain OCT technology, it is characterised in that: institute
The first motorized precision translation stage (5) stated is used to carry out dynamic focusing to sample (6)；To keep in dynamic focussing process, spectroscope (3)
It is needed to sample (6) focal plane and to the equivalent optical path of reference mirror (9), the first motorized precision translation stage (5) and the second motorized precision translation stage (11)
Synchronization action simultaneously meets relationship: nair△lr=nsam△lf, in which: △ lrWith △ lfRespectively the axial of reference mirror and focal plane is moved
Momentum, nairAnd nsamThe respectively refractive index of air and sample.
5. the real-time angiographic method based on whole audience time domain OCT technology, it is characterised in that: including blood vessel dynamic arthrography and blood vessel
Two methods of structure radiography；
Blood vessel dynamic arthrography method, comprising the following steps:
Step 11: activation system carries out sampling time interval parameter setting；
Step 12: by the axial movement of the first motorized precision translation stage (5) and the second motorized precision translation stage (11), to select to sample
(6) depth location being imaged；Hereafter the second motorized precision translation stage (11) remains stationary, and actuator (10) is moved with reference mirror (9)
Mutually or Carrier Modulation operates, while being spaced certain layer in acquisition sample (6) of interference letter according to set time by two-dimensional camera (13)
Number；
Step 13: to the interference signal of two-dimensional camera (13) acquisition, at phase shift or Carrier Modulation signal extracting algorithm
Reason specifically includes amplitude and phase information to obtain certain layer of field distribution information changed over time in sample (6)；
Step 14: to the field distribution information changed over time, using radiography data processing method, to obtain certain in sample (6)
The multidate information that the vascular system of layer changes over time；
Step 15: generating and show the dynamic image of vascular system；
Step 16: it repeats step 12 to step 15, the first motorized precision translation stage (5) and the second motorized precision translation stage (11) and moves axially,
Select other layers of progress blood vessel dynamic arthrography imaging in sample (6)；Or terminate program；
Blood vessel structure angiographic method, comprising the following steps:
Step 21: activation system carries out imaging depth range, sampling interval and scanning speed parameter setting；
Step 22: the second motorized precision translation stage (11) axially consecutive scanning, the first motorized precision translation stage (5) carry out dynamic focusing, actuate
Device (10) makees phase shift with reference mirror (9) or Carrier Modulation operates, and two-dimensional camera (13) acquires the interference letter in sample (6) simultaneously
Number；
Step 23: to the interference signal of two-dimensional camera (13) acquisition, at phase shift or Carrier Modulation signal extracting algorithm
Reason, to obtain the field distribution information of sample (6) interior different layers, specifically includes amplitude and phase information；
Step 24: to the field distribution information of sample (6) interior adjacent layer, using radiography data processing method, to obtain sample (6)
The structural information of interior vascular system；
Step 25: generating and show the structural images of vascular system；
Step 26: change imaging parameters repeat step 22 to step 25, re-start blood vessel structure contrast imaging；Or terminate journey
Sequence.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN112168136A (en) * | 2020-09-27 | 2021-01-05 | 中国科学院光电技术研究所 | Scanning-free three-dimensional optical coherence tomography angiography and tissue structure imaging system and method |
| CN112450867A (en) * | 2020-11-12 | 2021-03-09 | 温州医科大学 | Continuous variable focus and variable optical path biological measuring instrument and eye axis measuring method |
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2019
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| Title |
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| 杨亚良: "全场光学相干层析成像研究", 《中国优秀博硕士学位论文全文数据库（博士）基础科学辑》 * |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN112168136A (en) * | 2020-09-27 | 2021-01-05 | 中国科学院光电技术研究所 | Scanning-free three-dimensional optical coherence tomography angiography and tissue structure imaging system and method |
| CN112168136B (en) * | 2020-09-27 | 2023-08-11 | 中国科学院光电技术研究所 | Scanning-free three-dimensional optical coherence tomography angiography and tissue structure imaging system and method |
| CN112450867A (en) * | 2020-11-12 | 2021-03-09 | 温州医科大学 | Continuous variable focus and variable optical path biological measuring instrument and eye axis measuring method |
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