CN109984365A - A kind of biotinylation pulullan polysaccharide derivative is used to improve the purposes of the cigarette shreds biotin rate of transform - Google Patents
A kind of biotinylation pulullan polysaccharide derivative is used to improve the purposes of the cigarette shreds biotin rate of transform Download PDFInfo
- Publication number
- CN109984365A CN109984365A CN201910394411.6A CN201910394411A CN109984365A CN 109984365 A CN109984365 A CN 109984365A CN 201910394411 A CN201910394411 A CN 201910394411A CN 109984365 A CN109984365 A CN 109984365A
- Authority
- CN
- China
- Prior art keywords
- biotin
- biotinylation
- pulullan polysaccharide
- cigarette
- transform
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- A—HUMAN NECESSITIES
- A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
- A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
- A24B15/00—Chemical features or treatment of tobacco; Tobacco substitutes, e.g. in liquid form
- A24B15/18—Treatment of tobacco products or tobacco substitutes
-
- A—HUMAN NECESSITIES
- A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
- A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
- A24B3/00—Preparing tobacco in the factory
- A24B3/12—Steaming, curing, or flavouring tobacco
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0006—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid
- C08B37/0009—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid alpha-D-Glucans, e.g. polydextrose, alternan, glycogen; (alpha-1,4)(alpha-1,6)-D-Glucans; (alpha-1,3)(alpha-1,4)-D-Glucans, e.g. isolichenan or nigeran; (alpha-1,4)-D-Glucans; (alpha-1,3)-D-Glucans, e.g. pseudonigeran; Derivatives thereof
- C08B37/0018—Pullulan, i.e. (alpha-1,4)(alpha-1,6)-D-glucan; Derivatives thereof
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Materials Engineering (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Polymers & Plastics (AREA)
- Organic Chemistry (AREA)
- General Chemical & Material Sciences (AREA)
- Manufacture Of Tobacco Products (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
Abstract
The present invention discloses the purposes that a kind of biotinylation pulullan polysaccharide derivative is used to improve the cigarette shreds biotin rate of transform.The biotinylation pulullan polysaccharide derivative added in pipe tobacco of the present invention relative to addition biotin monomer more resistant to high temperature, be adapted to high temperature hot procedure, the biotin rate of transform of pipe tobacco can be significantly improved by the biotinylation pulullan polysaccharide derivative added in the tobacco.Meanwhile the pipe tobacco of biotinylation pulullan polysaccharide derivative that adds of the present invention roll made of cigarette add in thermogenetic main flume that the biotin rate of transform is high, really enhance the biotin functional characteristic of cigarette.
Description
Technical field
The invention belongs to tobacco industry application fields, and in particular to a kind of biotinylation pulullan polysaccharide derivative is for mentioning
The purposes of the high cigarette shreds biotin rate of transform.
Background technique
Biotin (Biotin) is one of B family vitamin, also known as biotin, vitamin B7, biotin (Coenzyme R),
Molecular weight is 244Da, is to synthesize ascorbic necessary material and a kind of maintenance human body natural growth, development and normal human
The necessary nutrient of functional health.The cigarette that Cigarette made of addition biotin can be such that it rolls in cigarette shreds generates
There is biotin, to make Cigarette that there is biotin functional characteristic in gas.At present at home cigarette enterprise mainly by
Biotin monomer is added in pipe tobacco to improve the content of biotin in cigarette shreds, but is seen with practical conditions, and biotin list is added
Pipe tobacco after body, after hot-working coiling process obtains Cigarette, in the pipe tobacco of Cigarette, the biotin rate of transform is simultaneously
It is not high, and the biotin rate of transform of the Cigarette in aspiration procedure in main flume is low, wherein and biotin shifts in pipe tobacco
Additional biological cellulose content × 100% of contained biology cellulose content/pipe tobacco in rate=pipe tobacco, the biotin rate of transform in main flume
The biological cellulose content retained in pipe tobacco before contained biological cellulose content/pipe tobacco burns in the main flume that the burning of=pipe tobacco generates ×
100%.
Therefore, it is intended that Cigarette has good biotin functional characteristic, the most fundamental investigated in Cigarette
The biological cellulose content that pipe tobacco is retained not is biological cellulose content in the pipe tobacco after adding biotin monomer, and Cigarette occurs
Pipe tobacco in biotin retain the not high reason of content and may is that, in the actual production process, addition biology in the tobacco
It is not the drying process for being directly used in rolled cigarette, but passing through high temperature (130-180 DEG C), so adding in pipe tobacco after element
The biotin added whether high temperature resistant, whether be adapted to high temperature hot procedure, be in the pipe tobacco of Cigarette made of rolling
The major reason of biotin retention content height.
Therefore, a kind of method for developing cigarette shreds biotin rate of transform for cigarette industry field just seems especially heavy
It wants.
Summary of the invention
The present invention provides a kind of biotinylation pulullan polysaccharide derivative for improving the cigarette shreds biotin rate of transform
Purposes, the preparation method of the biotinylation pulullan polysaccharide derivative the following steps are included:
(1) anhydrous dimethyl sulphoxide, biotin, dicyclohexylcarbodiimide and 4- diformazan are sequentially added in conical flask
Base aminopyridine reacts 0.5-1h at room temperature, and pulullan polysaccharide is then added, and 0.5-1h is reacted in continuation at room temperature, until white
Color contamination absurd creature is precipitated, and obtains turbid solution；
(2) turbid solution obtained in filtration step (1), white casse object is filtered to remove, and is collected filtrate and is put into retention
Molecular weight is that deionized water dialysis 4-8d is added in the bag filter of 9000Da, replaces a deionized water, dialysis knot every 0.5h
Shu Hou, suction filtration obtain white precipitate, successively wash white precipitate with methylene chloride and deionized water, dry, obtain biotinylation
Pulullan polysaccharide derivative.
Preferably, in step (1), anhydrous dimethyl sulphoxide, biotin, dicyclohexylcarbodiimide and 4- dimethylamino
The molar ratio of yl pyridines is 704:10:20:1.
Preferably, in step (1), the molar ratio of biotin and pulullan polysaccharide is 1/2-1.
Preferably, biotinylation pulullan polysaccharide derivative is added in cigarette shreds, the biotinylation of addition is general
The quality of Shandong orchid polysaccharide derivates accounts for the 1/10~1/15 of tobacco quality.
Wherein, add the pipe tobacco of the biotinylation pulullan polysaccharide derivative dry 5 in 100~150 DEG C of baking ovens~
10min, makes moisture in cut tobacco be maintained at 12 ± 1%, then places it in 22 ± 1 DEG C of temperature, puts down in 60 ± 2% environment of relative humidity
Weigh 24~48h, then carry out high temperature cut tobacco drying, weigh 25g balance after pipe tobacco be placed in 150~180 DEG C of baking ovens dry 5~
10min obtains the pipe tobacco for cigarette.Wherein, the pipe tobacco after balance is placed in 150~180 DEG C of baking ovens and dries 5~10min just
Be in investigating pipe tobacco the biotin that adds whether high temperature resistant, whether be adapted to high temperature hot procedure.
Wherein, by cellulose content biological in high effective liquid chromatography for measuring sample, in the pipe tobacco biotin rate of transform=pipe tobacco
Contained additional biological cellulose content × 100% of biology cellulose content/pipe tobacco.Then it will be rolled by the pipe tobacco of high temperature cut tobacco drying
Cigarette heats it using the cigarette smoking set that do not burn is heated, and collects the main flume of cigarette generation, raw in main flume
The life retained in pipe tobacco before contained biological cellulose content/pipe tobacco burns in the main flume that the object element rate of transform=pipe tobacco burning generates
Object cellulose content × 100%.
Compared with the existing technology, the invention has the following advantages:
1, the biotinylation pulullan polysaccharide derivative added in pipe tobacco of the present invention relative to addition biotin monomer more
High temperature resistant is adapted to high temperature hot procedure, after high temperature cut tobacco drying, is adding biology in the pipe tobacco of biotin monomer
The plain rate of transform is only 38.02%, and adds the biotin rate of transform in the pipe tobacco of biotinylation pulullan polysaccharide derivative and be
89.34%, turned by the biotin that the biotinylation pulullan polysaccharide derivative added in the tobacco can significantly improve pipe tobacco
Shifting rate.
2, cigarette made of the pipe tobacco for the biotinylation pulullan polysaccharide derivative that the present invention adds rolls adds thermogenetic
In main flume the biotin rate of transform be addition biotin monomer pipe tobacco roll made of cigarette add thermogenetic mainstream smoke
1.59 times of the aeroplankton element rate of transform, and cigarette of the present invention is during heating, the biotin in pipe tobacco is not destroyed,
But it is evaporated with main flume.And biotin is a kind of maintenance human body natural growth, development and normal human's functional health
Necessary nutrient, the raising of the biotin rate of transform in main flume, is only and really enhances the biotin functional characteristic of cigarette.
Detailed description of the invention
Nothing
Specific embodiment
In order to make the objectives, technical solutions, and advantages of the present invention clearer, with reference to embodiments, to the present invention
It is further elaborated.It should be appreciated that the specific embodiments described herein are merely illustrative of the present invention, it is not used to
Limit the present invention.
Comparative example 1
The pipe tobacco for taking 100g pipe tobacco uniformly to add 10g biotin monomer dries 5~10min in 100~150 DEG C of baking ovens,
So that moisture in cut tobacco is maintained at 12 ± 1%, then place it in 22 ± 1 DEG C of temperature, balance 24 in 60 ± 2% environment of relative humidity~
Then 48h carries out high temperature cut tobacco drying, the pipe tobacco after weighing 25g balance is placed in 5~10min of baking in 150~180 DEG C of baking ovens,
Obtain pipe tobacco comparative sample 1.
Embodiment 1
100g pipe tobacco is taken uniformly to add the pipe tobacco of 10g biotinylation pulullan polysaccharide derivative in 100~150 DEG C of baking ovens
Dry 5~10min, makes moisture in cut tobacco be maintained at 12 ± 1%, then places it in 22 ± 1 DEG C of temperature, relative humidity 60 ± 2%
24~48h is balanced in environment, then carries out high temperature cut tobacco drying, and the pipe tobacco after weighing 25g balance is placed in 150~180 DEG C of baking ovens
5~10min of middle baking, obtains tobacco sample 1.
Wherein, biotinylation pulullan polysaccharide derivative the preparation method is as follows: being sequentially added in 200mL conical flask
100mL anhydrous dimethyl sulphoxide, 2mmol biotin, 2.4mmol dicyclohexylcarbodiimide and 0.2 mmol4- dimethylamino
Pyridine reacts 0.7h at room temperature, rear that 4mmol pulullan polysaccharide is added, and continuation is reacted at room temperature, until white casse object is analysed
Out, white casse object is filtered to remove, puts the filtrate into the bag filter that molecular cut off is 9000Da, 3L deionized water is added
Dialyse 6d, primary every 0.5h replacement dialyzate.After dialysis, white precipitate is filtered, successively uses methylene chloride and deionization
The white solid obtained on water washing filter paper, it is that biotinylation pulullan polysaccharide is derivative that white powder product is obtained after dry
Object.
By cellulose content biological in high effective liquid chromatography for measuring pipe tobacco comparative sample 1 and tobacco sample 1, setting high temperature dries silk
Biotin is consistent with content in tobacco sample 1 in comparative sample 1 before process, and is denoted as 100.00%, and after high temperature cut tobacco drying,
The additional biological cellulose content of the cigarette shreds of contained biology cellulose content/homogenous quantities in the cigarette shreds biotin rate of transform=cigarette shreds
× 100%.The different tobacco sample biotin rate of transform are shown in Table 1.
Then pipe tobacco comparative sample 1 and tobacco sample 1 are rolled into Cigarette, obtains cigarette comparative sample 1 and cigarette sample
2, cigarette comparative sample 1 and cigarette sample 2 are heated using the cigarette smoking set that do not burn is heated, and collect the mainstream of cigarette generation
Flue gas, the sum of contained biological cellulose content in the main flume that the biotin rate of transform=cigarette pipe tobacco in main flume generates/
Additional biological cellulose content × 100% of pipe tobacco identical with the tobacco quality of Cigarette.The mainstream smoke that different cigarette samples generate
The gas biotin rate of transform is shown in Table 2.
The different tobacco sample biotin rate of transform of table 1
As it can be seen from table 1 after the biotin monomer and biotinylation pulullan polysaccharide derivative of addition homogenous quantities
Pipe tobacco, before high temperature cut tobacco drying, the biotin rate of transform does not have a notable difference in pipe tobacco, but after high temperature cut tobacco drying,
Adding the biotin rate of transform in the pipe tobacco comparative sample 1 of biotin monomer is only 38.02%, and adds biotinylation pulullan polysaccharide
The biotin rate of transform is 89.34% in the tobacco sample 1 of derivative, and more with the pipe tobacco biotin rate of transform before high temperature cut tobacco drying
It is close, thus the biotinylation pulullan polysaccharide derivative added in pipe tobacco more resistant to high temperature, be adapted to high temperature hot procedure,
That is, the biotin transfer of pipe tobacco can be improved by the biotinylation pulullan polysaccharide derivative added in the tobacco
Rate.
The main flume biotin rate of transform that the different cigarette samples of table 2 generate
| Sample | The biotin rate of transform (%) |
| Cigarette comparative sample 1 | 8.04 |
| Cigarette sample 1 | 12.79 |
From table 2 it can be seen that the biotin rate of transform is in cigarette sample 1 of the present invention plus thermogenetic main flume
12.79%, i.e. cigarette sample 1 has 12.79% biotin in tobacco sample 1 with flue gas during heating generates flue gas
It evaporates, and the biotin rate of transform is that the heating of cigarette comparative sample 1 produces in cigarette sample 1 of the present invention plus thermogenetic main flume
1.59 times of the biotin rate of transform in raw main flume, that is to say, that cigarette of the present invention heating generate flue gas during,
The biotin retained in pipe tobacco is not destroyed, but is evaporated with main flume, the biotin rate of transform in main flume
It improves, is only and really enhances the biotin functional characteristic of cigarette.
Claims (3)
1. a kind of biotinylation pulullan polysaccharide derivative is used to improve the purposes of the cigarette shreds biotin rate of transform, feature exists
In, the biotinylation pulullan polysaccharide derivative preparation method the following steps are included:
(1) anhydrous dimethyl sulphoxide, biotin, dicyclohexylcarbodiimide and 4- dimethylamino are sequentially added in conical flask
Pyridine reacts 0.5-1h at room temperature, and pulullan polysaccharide is then added, and 0.5-1h is reacted in continuation at room temperature, until white casse
Object is precipitated, and obtains turbid solution；
(2) turbid solution obtained in filtration step (1), white casse object is filtered to remove, and is collected filtrate and is put into retention molecule
Amount is that deionized water dialysis 4-8d is added in the bag filter of 9000Da, replaces a deionized water every 0.5h, dialysis terminates
Afterwards, it filters and obtains white precipitate, successively wash white precipitate with methylene chloride and deionized water, it is dry, it is general to obtain biotinylation
Shandong orchid polysaccharide derivates.
2. the method according to claim 1, wherein in step (1), anhydrous dimethyl sulphoxide, biotin, two rings
The molar ratio of hexyl carbodiimide and 4-dimethylaminopyridine is 704:10:20:1.
3. the method according to claim 1, wherein in step (1), the molar ratio of biotin and pulullan polysaccharide
For 1/2-1.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910394411.6A CN109984365B (en) | 2019-05-13 | 2019-05-13 | Application of biotinylation pullulan polysaccharide derivative in improving biotin transfer rate of cigarette cut tobacco |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910394411.6A CN109984365B (en) | 2019-05-13 | 2019-05-13 | Application of biotinylation pullulan polysaccharide derivative in improving biotin transfer rate of cigarette cut tobacco |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN109984365A true CN109984365A (en) | 2019-07-09 |
| CN109984365B CN109984365B (en) | 2021-07-09 |
Family
ID=67136549
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910394411.6A Active CN109984365B (en) | 2019-05-13 | 2019-05-13 | Application of biotinylation pullulan polysaccharide derivative in improving biotin transfer rate of cigarette cut tobacco |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109984365B (en) |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101041075A (en) * | 2007-04-20 | 2007-09-26 | 中国医学科学院生物医学工程研究所 | Biotinylated pulullan polysaccharide nanometer grains and the preparation method |
| CN102432921A (en) * | 2011-09-16 | 2012-05-02 | 中国烟草总公司郑州烟草研究院 | Pullulan additive and application thereof in cigarettes |
| CN104432492A (en) * | 2014-11-03 | 2015-03-25 | 云南中烟工业有限责任公司 | Application of Pulullan or compound of Pulullan in cigarette |
| CN104770858A (en) * | 2015-02-11 | 2015-07-15 | 贵州中烟工业有限责任公司 | Gastric mucosa protected mouth-holding type smokeless tobacco product and preparing method thereof |
| US20160050969A1 (en) * | 2014-08-25 | 2016-02-25 | Kedar Prasad | Micronutrient formulation in electronic cigarettes |
| CN105595404A (en) * | 2015-10-20 | 2016-05-25 | 贵州中烟工业有限责任公司 | Tobacco buccal tablet and preparation method thereof |
| CN108338360A (en) * | 2018-01-31 | 2018-07-31 | 陕西海斯夫生物工程有限公司 | A kind of high temperature resistant distinctive unsaturated fat acid lipid microcapsule powder and preparation method thereof |
-
2019
- 2019-05-13 CN CN201910394411.6A patent/CN109984365B/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101041075A (en) * | 2007-04-20 | 2007-09-26 | 中国医学科学院生物医学工程研究所 | Biotinylated pulullan polysaccharide nanometer grains and the preparation method |
| CN102432921A (en) * | 2011-09-16 | 2012-05-02 | 中国烟草总公司郑州烟草研究院 | Pullulan additive and application thereof in cigarettes |
| US20160050969A1 (en) * | 2014-08-25 | 2016-02-25 | Kedar Prasad | Micronutrient formulation in electronic cigarettes |
| CN104432492A (en) * | 2014-11-03 | 2015-03-25 | 云南中烟工业有限责任公司 | Application of Pulullan or compound of Pulullan in cigarette |
| CN104770858A (en) * | 2015-02-11 | 2015-07-15 | 贵州中烟工业有限责任公司 | Gastric mucosa protected mouth-holding type smokeless tobacco product and preparing method thereof |
| CN105595404A (en) * | 2015-10-20 | 2016-05-25 | 贵州中烟工业有限责任公司 | Tobacco buccal tablet and preparation method thereof |
| CN108338360A (en) * | 2018-01-31 | 2018-07-31 | 陕西海斯夫生物工程有限公司 | A kind of high temperature resistant distinctive unsaturated fat acid lipid microcapsule powder and preparation method thereof |
Non-Patent Citations (2)
| Title |
|---|
| 史艳萍等: "生物素化普鲁兰多糖纳米颗粒制备与表征", 《功能材料》 * |
| 潘道东等: "《功能性食品添加剂》", 31 January 2006, 北京：中国轻工业出版社 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN109984365B (en) | 2021-07-09 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Nagahama et al. | Novel biodegradable chitin membranes for tissue engineering applications | |
| Singh et al. | Synthesis and evaluation of physicochemical properties of cross-linked sago starch | |
| CN107319628A (en) | One kind is applied to heat incombustible tobacco matrixes and preparation method thereof | |
| CN108912340B (en) | Moisture-retaining cigarette paper and preparation method thereof | |
| CN105693871B (en) | A kind of preparation method of resistant dextrin | |
| CN106690395B (en) | A kind of bacterium enzyme mix preparation of the SMXP-58 containing bacillus subtilis strain | |
| CN202104213U (en) | Reconstituted tobacco leaf | |
| CN106690402A (en) | Application of bacillus subtilis SMXP-58 strain in tobacco stem | |
| CN105146712A (en) | Method for improving cigarette moisturizing and aroma-improving effects by utilizing red rose | |
| Singh et al. | Synthesis and evaluation of physicochemical properties of cross‐linked Phaseolus aconitifolius starch | |
| CN109984365A (en) | A kind of biotinylation pulullan polysaccharide derivative is used to improve the purposes of the cigarette shreds biotin rate of transform | |
| CN102266116B (en) | Reconstituted tobacco leaf and preparation method and application thereof | |
| CN103284327B (en) | Tobacco paper filter stick combining bamboo fiber and preparation method of tobacco paper filter stick | |
| CN108968138A (en) | For improving the essence and its preparation method and application for heating smoke of tobacco state of not burning | |
| CN111374343A (en) | Reconstituted tobacco for low-combustion-temperature cigarettes and preparation method thereof | |
| CN109275934A (en) | A kind of processing method suitable for heating pipe tobacco under not combustion state | |
| CN107927899A (en) | A kind of method for reducing carbon monoxide release amount of papermaking reconstituted tobacco | |
| CN104351689A (en) | Gluten-protein-free steamed sponge cakes produced by sweet potato coarse dietary fibers and preparation method thereof | |
| CN108425269A (en) | A kind of cigarette paper and preparation method thereof added with tangerine peel powder | |
| CN107243320A (en) | It is a kind of to be used to heat zeolite matrix of non-combustion-type tobacco product and preparation method thereof | |
| US20150201671A1 (en) | Cigarette paper additive having harm reduction function and application thereof | |
| CN107515170B (en) | Method for measuring content of hot water soluble substances | |
| CN111213904A (en) | Treatment method of inapplicable fresh tobacco leaves | |
| Hidayati et al. | Optimization of Extraction Methods and Dyeing Standarization of Nila Leaves (Indigoferatinctoria Linn.) as Natural Dyes | |
| CN110093815B (en) | Tipping paper capable of reducing tar content of cigarette paper |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |