CN109976413A - A kind of optical microscopy temperature control system and method - Google Patents
A kind of optical microscopy temperature control system and method Download PDFInfo
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- CN109976413A CN109976413A CN201910367742.0A CN201910367742A CN109976413A CN 109976413 A CN109976413 A CN 109976413A CN 201910367742 A CN201910367742 A CN 201910367742A CN 109976413 A CN109976413 A CN 109976413A
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- microcontroller
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- solenoid valve
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- G—PHYSICS
- G05—CONTROLLING; REGULATING
- G05D—SYSTEMS FOR CONTROLLING OR REGULATING NON-ELECTRIC VARIABLES
- G05D23/00—Control of temperature
- G05D23/19—Control of temperature characterised by the use of electric means
- G05D23/30—Automatic controllers with an auxiliary heating device affecting the sensing element, e.g. for anticipating change of temperature
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- Engineering & Computer Science (AREA)
- Automation & Control Theory (AREA)
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Abstract
The present invention provides a kind of optical microscopy temperature control systems, including microcontroller, PWM voltage regulating module, heat refrigeration unit, temperature sensor and display, the output end of the microcontroller is connect with the input terminal of the PWM voltage regulating module, the microcontroller inputs trigger signal to the PWM voltage regulating module, the output end of the PWM voltage regulating module is connect with the input terminal of the heating refrigeration unit, the regulation of voltage is realized by the PWM voltage regulating module, reach the control heating refrigeration unit heating power or refrigeration work consumption, the output end of the temperature sensor is connect with the input terminal of the microcontroller, the temperature sensor acquires the temperature of the heating refrigeration unit and in real time to the microcontroller input feedback signal.The present invention also provides a kind of optical microscopy temprature control methods.The beneficial effects of the present invention are: providing dedicated temperature control system for optical microscopy, be conducive to meet requirement of experiment.
Description
Technical field
The present invention relates to optical microscopy more particularly to a kind of optical microscopy temperature control systems and method.
Background technique
Traditional optical microscopy lacks temperature control system, is unable to satisfy requirement of experiment.
Summary of the invention
In order to solve the problems in the prior art, the present invention provides a kind of optical microscopy temperature control system and sides
Method.
The present invention provides a kind of optical microscopy temperature control systems, including microcontroller, PWM voltage regulating module, heating
The input terminal of refrigeration unit, temperature sensor and display, the output end of the microcontroller and the PWM voltage regulating module connects
It connects, the microcontroller inputs trigger signal to the PWM voltage regulating module, and the output end of the PWM voltage regulating module adds with described
The input terminal of refrigeration heat unit connects, and the regulation of voltage is realized by the PWM voltage regulating module, reaches the control heating refrigeration
Unit heating power or refrigeration work consumption, the output end of the temperature sensor are connect with the input terminal of the microcontroller, institute
State temperature sensor acquire in real time it is described heating refrigeration unit temperature and to the microcontroller input feedback signal, it is described micro-
Controller is connect with the display.
As a further improvement of the present invention, the heating refrigeration unit includes heat source, cold source, the first solenoid valve, second
Solenoid valve, third solenoid valve, the 4th solenoid valve, the first triple valve, the second triple valve and the copper billet that exchanges heat, set on the heat exchange copper billet
There are first interface and second interface, when the first interface is entrance, then the second interface is outlet, is connect when described second
When mouth is entrance, then the first interface is outlet, and the output end of the heat source passes sequentially through first solenoid valve, the one or three
Port valve is connect with the first interface of the heat exchange copper billet, and the input terminal of the heat source passes sequentially through the second solenoid valve, second
Triple valve is connect with the second interface of the heat exchange copper billet, and the output end of the cold source passes sequentially through the third solenoid valve, the
Two triple valves with it is described heat exchange copper billet second interface connect, the input terminal of the cold source pass sequentially through the 4th solenoid valve,
First triple valve is connect with the first interface of the heat exchange copper billet.
As a further improvement of the present invention, the transducing part of the temperature sensor is matched with the heat exchange copper billet.
As a further improvement of the present invention, the heat source, be equipped with heat transferring medium in cold source.
As a further improvement of the present invention, the optical microscopy temperature control system further includes computer, described micro-
Controller is connect by serial ports with the computer, and data are passed to computer in real time and saved by the microcontroller, for the later period
Data analysis.
As a further improvement of the present invention, the model STM 32 of the microcontroller.
As a further improvement of the present invention, the temperature sensor is thermocouple.
The present invention also provides a kind of optical microscopy temprature control methods, and PWM wave is input to PWM by microcontroller
Voltage regulating module, by control PWM wave duty ratio realize voltage regulation, reach control heating refrigeration unit heating power or
Real time temperature is acquired by thermocouple and is passed in microcontroller by refrigeration work consumption, realizes the closed-loop control of temperature, microcontroller
Real time information is shown in LCD screen, while by touch screen, realizing the setting of different experiments parameter and being passed to microcontroller
In device, by opening heat source or the corresponding water pump of cold source and corresponding solenoid valve, passing through to set temperature and real time temperature comparison
Microcontroller is connected by serial ports with computer, and data are passed in computer in real time and are saved, are analyzed for later data.
As a further improvement of the present invention, the setting process of experiment parameter is as follows:
S1, beginning;
S2, it chooses whether to adjust temperature with cyclically-varying;
S3, set temperature;
S4, choose whether setting time, if it is not, then set temperature changes speed, if it is, setting by a upper temperature or
The time required to room temperature to the set temperature;
S5, it chooses whether to add next temperature, if it is, return step S3, if it is not, then terminating.
The beneficial effects of the present invention are: through the above scheme, dedicated temperature control system is provided for optical microscopy,
Be conducive to meet requirement of experiment.
Detailed description of the invention
Fig. 1 is a kind of functional block diagram of optical microscopy temperature control system of the present invention.
Fig. 2 is a kind of setting procedure chart of the experiment parameter of optical microscopy temprature control method of the present invention.
Fig. 3 is a kind of schematic diagram of the heating refrigeration unit of optical microscopy temprature control method of the present invention.
Specific embodiment
The invention will be further described for explanation and specific embodiment with reference to the accompanying drawing.
As shown in Figure 1 to Figure 3, a kind of optical microscopy temperature control system, including microcontroller (MCU) 1, PWM pressure regulation
Module 3, heating refrigeration unit 4, temperature sensor 5 and display, the output end of the microcontroller 1 and the PWM pressure regulation mould
The input terminal of block 3 connects, and the microcontroller 1 inputs trigger signal, the PWM voltage regulating module 3 to the PWM voltage regulating module 3
Output end with it is described heating refrigeration unit 4 input terminal connect, by the PWM voltage regulating module 3 realization voltage regulation, reach
To control 4 heating power of heating refrigeration unit or refrigeration work consumption, the output end and the micro-control of the temperature sensor 5
The input terminal of device 1 processed connects, and the temperature sensor 5 acquires the temperature of the heating refrigeration unit 4 and in real time to the micro-control
1 input feedback signal of device processed, the microcontroller 1 are connect with the display, and display is preferably LCD screen 8.
As shown in Figure 1 to Figure 3, the heating refrigeration unit includes heat source 41, cold source 42, first the 71, second electricity of solenoid valve
Magnet valve 72, third solenoid valve 73, the 4th solenoid valve 74, the first triple valve 43, the second triple valve 44 and heat exchange copper billet 45, it is described to change
Hot copper billet 45 is equipped with first interface 451 and second interface 452, when the first interface 451 is entrance, then described second connects
Mouthfuls 452 be outlet, and when the second interface 452 is entrance, then the first interface 451 is outlet, the heat source 41 it is defeated
Outlet passes sequentially through first solenoid valve 71, the first triple valve 43 is connect with the first interface 451 of the heat exchange copper billet 45, institute
The input terminal for stating heat source 41 passes sequentially through the second solenoid valve 72, the second triple valve 44 connects with the second of the heat exchange copper billet 45
Mouth 452 connects, and the output end of the cold source 42 passes sequentially through the third solenoid valve 73, the second triple valve 44 and the heat exchange copper
The second interface 452 of block 45 connects, and the input terminal of the cold source 42 passes sequentially through the 4th solenoid valve 74, the first triple valve 43
It is connect with the first interface 451 of the heat exchange copper billet 45, the first solenoid valve 71, second solenoid valve 72 are the corresponding electromagnetism of heat source 41
Valve, and third solenoid valve 73, the 4th solenoid valve 74 are then the corresponding solenoid valve of cold source 42, the first solenoid valve 71, second solenoid valve
72, third solenoid valve 73, the 4th solenoid valve 74 constitute electromagnetic valve component 7, can be controlled respectively by microcontroller 1.
As shown in Figure 1 to Figure 3, the transducing part of the temperature sensor 5 is matched with the heat exchange copper billet 45.
As shown in Figure 1 to Figure 3, the heat source 41, be equipped with heat transferring medium in cold source 42, heat transferring medium is preferably water,
Setting heating sheet generates hot water, cooling piece is arranged in cold source 42, for freezing, generate cold water, heat for heating in heat source 41
The correspondence of source 41 is equipped with the first water pump, fills the water for heat source 41, and the correspondence of cold source 42 is equipped with the second water pump, is used for cold source 42
Water filling, the first water pump, the second water pump constitute water pump component 6, can be controlled respectively by microcontroller 1.
As shown in Figure 1 to Figure 3, the optical microscopy temperature control system further includes computer 2, the microcontroller 1
It is connect by serial ports with the computer 2, data are passed to computer 2 in real time and saved by the microcontroller 1, for later data
Analysis.
As shown in Figure 1 to Figure 3, the model STM 32 of the microcontroller 1.
As shown in Figure 1 to Figure 3, the temperature sensor 5 is thermocouple.
As shown in Figure 1 to Figure 3, the present invention also provides a kind of optical microscopy temprature control method, pass through microcontroller 1
PWM wave is input to PWM voltage regulating module 3, the duty ratio by controlling PWM wave realizes the regulation of voltage, reaches control heating system
The heating power or refrigeration work consumption of cold unit 4, real time temperature is acquired and passed to by thermocouple in microcontroller 1, realizes temperature
Real time information is shown in LCD screen 8 by the closed-loop control of degree, microcontroller 1, while by touch screen, realizing different experiments
The setting of parameter is simultaneously passed in microcontroller 1, by opening heat source 41 or cold source 42 to set temperature and real time temperature comparison
Microcontroller 1 is connected by corresponding water pump and corresponding solenoid valve by serial ports with computer 2, and data are passed in real time and are calculated
It in machine 2 and saves, is analyzed for later data.
The setting process of experiment parameter is as follows:
S1, beginning;
S2, it chooses whether to adjust temperature with cyclically-varying;
S3, set temperature;
S4, choose whether setting time, if it is not, then set temperature changes speed, if it is, setting by a upper temperature or
The time required to room temperature to the set temperature;
S5, it chooses whether to add next temperature, if it is, return step S3, if it is not, then terminating.
User can set one to multiple target temperatures, Cooling rate or alternating temperature length of time on demand, if the period
Property temperature control.
A kind of optical microscopy temperature control system and method provided by the invention are used as microcontroller 1 using STM 32,
PWM wave is inputted into PWM voltage regulating module 3, the duty ratio by controlling PWM wave realizes the regulation of voltage, reaches control heating or system
Cold power.Real time temperature is acquired by thermocouple (MAX6675) and is passed in microcontroller 1, realizes the closed-loop control of temperature.
By opening heat source 41 or the corresponding water pump of cold source 42 and corresponding solenoid valve to set temperature and real time temperature comparison.Micro-control
Real time information is shown in LCD screen 8 by device 1 processed, while by touch screen, being realized the setting of different experiments parameter and being passed to
In microcontroller 1.Microcontroller 1 is connected with computer 2 by serial ports, data are passed in computer 1 in real time and are saved, are supplied
Later data analysis.
A kind of optical microscopy temperature control system and method provided by the invention ensure reality by heating refrigeration unit 4
Test desired rate temperature change.The first solenoid valve 71 and second solenoid valve 72 are opened by microcontroller 1, closes third electromagnetism
Valve 73 and the 4th solenoid valve 74, at this time in heat source 41 hot water will flow through heat exchange copper billet 45, finally flow into heat source 41, with heating sheet into
Row heat exchange reaches sample and is rapidly heated purpose.Similarly, by opening third solenoid valve 73 and the 4th solenoid valve 74, the is closed
One solenoid valve 71 and second solenoid valve 72 realize that the cold water in cold source 42 flows through heat exchange copper billet 45, realize the cold friendship with cooling piece
It changes, achievees the purpose that fast cooling.
A kind of optical microscopy temperature control system and method provided by the invention, have the advantage that
1, main control chip, program open source are used as using STM 32.Can be according to requirement of experiment, flexible reprogramming, and then it can be with
Meet the purpose of different experiments requirement.There is at low cost, the high advantage of cost performance simultaneously;
It 2, can be in real time by the data (such as real time temperature, heating power etc.) of acquisition and processing by way of serially printing
It is sent on computer, is processed convenient for post-processing.
The above content is a further detailed description of the present invention in conjunction with specific preferred embodiments, and it cannot be said that
Specific implementation of the invention is only limited to these instructions.For those of ordinary skill in the art to which the present invention belongs, exist
Under the premise of not departing from present inventive concept, a number of simple deductions or replacements can also be made, all shall be regarded as belonging to of the invention
Protection scope.
Claims (9)
1. a kind of optical microscopy temperature control system, it is characterised in that: including microcontroller, PWM voltage regulating module, heating refrigeration
Unit, temperature sensor and display, the output end of the microcontroller are connect with the input terminal of the PWM voltage regulating module, institute
It states microcontroller and inputs trigger signal to the PWM voltage regulating module, the output end of the PWM voltage regulating module and the heating are freezed
The input terminal of unit connects, and the regulation of voltage is realized by the PWM voltage regulating module, reaches the control heating refrigeration unit and adds
Thermal power or refrigeration work consumption, the output end of the temperature sensor are connect with the input terminal of the microcontroller, the temperature
Sensor acquires the temperature of the heating refrigeration unit and in real time to the microcontroller input feedback signal, the microcontroller
It is connect with the display.
2. optical microscopy temperature control system according to claim 1, it is characterised in that: the heating refrigeration unit packet
Include heat source, cold source, the first solenoid valve, second solenoid valve, third solenoid valve, the 4th solenoid valve, the first triple valve, the second triple valve
With heat exchange copper billet, the heat exchange copper billet is equipped with first interface and second interface, when the first interface is entrance, then described
Second interface is outlet, and when the second interface is entrance, then the first interface is outlet, the output end of the heat source according to
It is secondary to be connect by first solenoid valve, the first triple valve with the first interface of the heat exchange copper billet, the input terminal of the heat source
Pass sequentially through the second solenoid valve, the second triple valve is connect with the second interface of the heat exchange copper billet, the output of the cold source
End pass sequentially through the third solenoid valve, the second triple valve with it is described exchange heat copper billet second interface connect, the cold source it is defeated
Enter to hold to pass sequentially through the 4th solenoid valve, the first triple valve is connect with the first interface of the heat exchange copper billet.
3. optical microscopy temperature control system according to claim 2, it is characterised in that: the biography of the temperature sensor
Sense part is matched with the heat exchange copper billet.
4. optical microscopy temperature control system according to claim 2, it is characterised in that: in the heat source, cold source
Equipped with heat transferring medium.
5. optical microscopy temperature control system according to claim 1, it is characterised in that: the optical microscopy temperature
Control system further includes computer, and the microcontroller is connect by serial ports with the computer, and the microcontroller is by data
It is passed to computer in real time and saves, is analyzed for later data.
6. optical microscopy temperature control system according to claim 1, it is characterised in that: the model of the microcontroller
For STM 32.
7. optical microscopy temperature control system according to claim 1, it is characterised in that: the temperature sensor is heat
Galvanic couple.
8. a kind of optical microscopy temprature control method, it is characterised in that: PWM wave is input to PWM pressure regulation by microcontroller
Module, the duty ratio by controlling PWM wave realize the regulation of voltage, reach the heating power or refrigeration of control heating refrigeration unit
Real time temperature is acquired by thermocouple and is passed in microcontroller by power, realizes the closed-loop control of temperature, and microcontroller will be real
When information be shown in LCD screen, while by touch screen, realizing that the setting of different experiments parameter is simultaneously passed to microcontroller
In, by opening heat source or the corresponding water pump of cold source and corresponding solenoid valve, passing through string to set temperature and real time temperature comparison
Microcontroller is connected by mouth with computer, and data are passed in computer in real time and are saved, are analyzed for later data.
9. optical microscopy temprature control method according to claim 8, it is characterised in that: the setting process of experiment parameter
It is as follows:
S1, beginning;
S2, it chooses whether to adjust temperature with cyclically-varying;
S3, set temperature;
S4, choose whether setting time, if it is not, then set temperature changes speed, if it is, setting by a upper temperature or
The time required to room temperature to the set temperature;
S5, it chooses whether to add next temperature, if it is, return step S3, if it is not, then terminating.
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Application publication date: 20190705 |