CN109975401A - A kind of flowing pool device based on micro-cantilever beam sensor - Google Patents
A kind of flowing pool device based on micro-cantilever beam sensor Download PDFInfo
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- CN109975401A CN109975401A CN201910263682.8A CN201910263682A CN109975401A CN 109975401 A CN109975401 A CN 109975401A CN 201910263682 A CN201910263682 A CN 201910263682A CN 109975401 A CN109975401 A CN 109975401A
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J19/0053—Details of the reactor
- B01J19/0073—Sealings
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N29/00—Investigating or analysing materials by the use of ultrasonic, sonic or infrasonic waves; Visualisation of the interior of objects by transmitting ultrasonic or sonic waves through the object
- G01N29/02—Analysing fluids
- G01N29/022—Fluid sensors based on microsensors, e.g. quartz crystal-microbalance [QCM], surface acoustic wave [SAW] devices, tuning forks, cantilevers, flexural plate wave [FPW] devices
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N29/00—Investigating or analysing materials by the use of ultrasonic, sonic or infrasonic waves; Visualisation of the interior of objects by transmitting ultrasonic or sonic waves through the object
- G01N29/02—Analysing fluids
- G01N29/036—Analysing fluids by measuring frequency or resonance of acoustic waves
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54306—Solid-phase reaction mechanisms
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Abstract
The present invention relates to a kind of flowing pool device based on micro-cantilever beam sensor, including micro-cantilever clamping device, flow cell matrix, sealing ring, transparent glass sheet, metal cover board and solution inlet and outlet piping；The micro-cantilever clamping device is made of the preferable compressed spring of tensile property, hemisphere and micro-cantilever clamping device matrix；The flow cell matrix left and right ends are solution inlet and outlet, and micro-cantilever is placed in the groove on ladder；Sealing ring, sheet glass and metal cover board are successively installed on flow cell, manufacture a closed environment；While realizing micro-cantilever beam sensor detection function, the amount for the reaction solution being passed through in flow cell can be reduced, reduces waste, and provide certain protection for micro-cantilever.
Description
Technical field
The present invention relates to a kind of design of flowing pool device, specially a kind of flow cell dress based on micro-cantilever beam sensor
It sets.
Background technique
MEMS (MEMS) is a kind of technology with electric drive mechanical device of micron level.It is typical micro electronmechanical
The size of constituent system components is 1~100 μm, and the size of entire MEMS devices is in 20 μm~1mm.Utilize micro-cantilever
This simplest MEMS element, by detecting resonant frequency and the deflection of micro-cantilever, real-time detection micro-cantilever surface is special
Anisotropic biochemical reaction has many advantages, such as high sensitivity, without label, real-time detection.Existing flow cell fills both at home and abroad at present
Set that structure is complicated, it is cumbersome and be mostly made using material costly, micro-cantilever cannot be carried out in use process effective
Protection.And flow cell has vital effect with micro-cantilever in entire micro-cantilever beam sensor, probe molecule
It is mainly carried out on micro-cantilever surface with reacting for target molecule, it is therefore necessary to which it is simple to design a kind of structure, easy to operate
Flow pool device.
In document " An optimized measurement chamber for cantilever array
Measurements in liquid incorporating an automated sample handling system " in,
The flowing pool structure that author uses is extremely complex, and retained part is to use wedge by simply placing micro-cantilever in a groove
Shape block is pressed on micro-cantilever, and is screwed, and places micro-cantilever operating difficulties, and cannot have to micro-cantilever
The protection of effect.
Summary of the invention
The present invention is in order to avoid the shortcomings of the prior art, provide a kind of flow cell based on micro-cantilever beam sensor
Device, in the apparatus, while can be realized micro-cantilever beam sensor detection function, it is possible to reduce be passed through in flow cell
The amount of reaction solution reduces waste；In addition, applying a kind of novel micro-cantilever clamping device in flow cell, reduce numerous
Trivial operating procedure, and certain protection is provided for micro-cantilever.
The present invention solves technical problem and adopts the following technical scheme that
One flow cell matrix, the matrix are made of a material of the cylindrical body of stainless steel, are in three-level ladder inside flow cell
Shape, the groove for placing micro-cantilever are located on ladder, solid by screw with sealing ring, sheet glass and metal cover board above flow cell
It is scheduled on matrix, to constitute closed environment；
One micro-cantilever clamping device is made of compressed spring, hemisphere working portion and clamping matrix.
Compared with the prior art, the invention has the advantages that:
1. driving the fixed micro-cantilever of clamping device matrix by compressed spring, the trouble of frequently disassembly screw is eliminated,
Reduce the damage during the installation process to micro-cantilever；
2. the micro-cantilever of different-thickness, the micro-cantilever of any thickness can be suitable for since working portion is hemisphere
All be a face contact when any position of beam is contacted with hemisphere working portion, pressure passes through hemisphere center, and vertically to
Under.The sliding that not will cause micro-cantilever, ensure that stability.The compressed spring of rear end can adjust working portion to micro- well
The pressure of cantilever beam.
3. due to being in that three-level is ladder-like inside flow cell, lowest level volume very little can realize micro-cantilever beam sensor
While detection function, the amount for the reaction solution being passed through is reduced to reduce waste.
Detailed description of the invention
A kind of flowing pool device based on micro-cantilever beam sensor of Fig. 1 present invention
Fig. 2 flow cell matrix schematic diagram
Fig. 3 micro-cantilever clamping device matrix schematic diagram
Figure label: 1 flow cell matrix, 2 liquid outlets, 3 micro-cantilevers, 4 micro-cantilever clamping device matrixes, 4 boss, 6
Screw, 7 compressed springs, 8 metal cover boards, 9 inlets, 10 sheet glass, 11 screw holes, 12 micro-cantilever notches, 13 sealing rings, 14
Hemisphere, 15 short axles
Specific embodiment
The invention patent is described in detail below with reference to Fig. 1, Fig. 2 and Fig. 3, so as to technical staff's understanding.
Embodiment 1
As shown in Figure 1, Figure 2, Figure 3 shows, a kind of flowing pool device based on micro-cantilever beam sensor includes:
One flow cell matrix (1), the matrix are made of a material of the cylindrical body of stainless steel, are in three-level rank inside flow cell
Scalariform, the groove (12) for placing micro-cantilever are located on ladder, with sealing ring (13), sheet glass (10) and metal above flow cell
Cover board (8) is fixed on matrix (1) by screw (6), to constitute closed environment；
One micro-cantilever clamping device (4), by compressed spring (7), hemisphere working portion (14) and clamping matrix (4) group
At.
Embodiment 2
A kind of flowing pool device based on micro-cantilever beam sensor is using steps are as follows:
Micro-cantilever used in the device chooses the model of German Micro-motive company more popular on the market
The micro-cantilever of Octo500c, length and width are 2.5mm；
Unscrew screw (6) with screwdriver, removes metal cover board (8), sheet glass (10), sealing ring (13)；Pin micro-cantilever
Beam clamping device (4) rear portion, compressed spring (7) are compressed, and micro-cantilever clamping device (4) front hemisphere body portion (14) is raised
It rises, micro-cantilever (3) is put into micro-cantilever notch (12) at leisure with tweezers, carefully adjusts position, then
Slowly unclamp micro-cantilever clamping device (4) rear portion, compressed spring (7) rebound, micro-cantilever clamping device (4) front hemisphere
Partially (14) are pinned micro-cantilever (3), realize the effect of fixed micro-cantilever (3)；After cantilever beam (3) fixes, successively
Sealing ring (13), sheet glass (10), metal cover board (8), screw (6) are installed, to create the required closed environment of experiment,
Liquid outlet (2) is connected with external pipe respectively with inlet (9).
The present invention is illustrated by specific implementation process, without departing from the present invention, can be with
Various exchanges and same replacement are carried out to the present invention.Therefore, the present invention is not limited to disclosed specific embodiment, and should
The whole embodiments fallen within the scope of the appended claims.
Claims (4)
1. a kind of flowing pool device based on micro-cantilever beam sensor, characterized by comprising:
One flow cell matrix, the matrix are made of a material of the cylindrical body of stainless steel, ladder-like in three-level inside flow cell, put
The groove for setting micro-cantilever is located on ladder, and sealing ring, sheet glass and metal cover board are fixed by screws in above flow cell
On matrix, to constitute closed environment；
One micro-cantilever clamping device is made of compressed spring, hemisphere working portion and clamping matrix.
2. a kind of flowing pool device based on micro-cantilever beam sensor according to claim 1, it is characterised in that: flow cell
Inside is ladder-like in three-level, there is the boss of the groove and installation micro-cantilever clamping device for placing micro-cantilever.
3. a kind of flowing pool device based on micro-cantilever beam sensor according to claim 1, it is characterised in that: micro-cantilever
Beam clamping device front end working portion is hemisphere, and rear end is connected by compressed spring with flow cell matrix.
4. a kind of flowing pool device based on micro-cantilever beam sensor according to claim 1, it is characterised in that: flow cell
Top sealing ring, sheet glass and metal cover board are fixed by screws on matrix, constitute closed environment；Have on flow cell matrix
Pipeline for solution disengaging.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910263682.8A CN109975401A (en) | 2019-04-02 | 2019-04-02 | A kind of flowing pool device based on micro-cantilever beam sensor |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910263682.8A CN109975401A (en) | 2019-04-02 | 2019-04-02 | A kind of flowing pool device based on micro-cantilever beam sensor |
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| Publication Number | Publication Date |
|---|---|
| CN109975401A true CN109975401A (en) | 2019-07-05 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910263682.8A Pending CN109975401A (en) | 2019-04-02 | 2019-04-02 | A kind of flowing pool device based on micro-cantilever beam sensor |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109827904A (en) * | 2019-03-19 | 2019-05-31 | 安徽理工大学 | A kind of reaction pool device based on micro-cantilever beam sensor |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2100125A2 (en) * | 2006-11-28 | 2009-09-16 | Drexel University | Piezoelectric microcantilever sensors for biosensing |
| CN201511224U (en) * | 2009-09-18 | 2010-06-23 | 安徽星马汽车股份有限公司 | Bulk cement semitrailer sliding board fixing cramping apparatus |
| CN106312614A (en) * | 2015-06-18 | 2017-01-11 | 张家港市普飞特建材有限公司 | Fixture |
| CN206898826U (en) * | 2017-06-16 | 2018-01-19 | 绵阳二四机械有限公司 | A kind of clamping device applied to mechanical fitting production |
| CN108956587A (en) * | 2018-07-11 | 2018-12-07 | 安徽理工大学 | A method of ionic adsorption is monitored based on micro-cantilever sensing technology |
-
2019
- 2019-04-02 CN CN201910263682.8A patent/CN109975401A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2100125A2 (en) * | 2006-11-28 | 2009-09-16 | Drexel University | Piezoelectric microcantilever sensors for biosensing |
| CN201511224U (en) * | 2009-09-18 | 2010-06-23 | 安徽星马汽车股份有限公司 | Bulk cement semitrailer sliding board fixing cramping apparatus |
| CN106312614A (en) * | 2015-06-18 | 2017-01-11 | 张家港市普飞特建材有限公司 | Fixture |
| CN206898826U (en) * | 2017-06-16 | 2018-01-19 | 绵阳二四机械有限公司 | A kind of clamping device applied to mechanical fitting production |
| CN108956587A (en) * | 2018-07-11 | 2018-12-07 | 安徽理工大学 | A method of ionic adsorption is monitored based on micro-cantilever sensing technology |
Non-Patent Citations (2)
| Title |
|---|
| MICHAEL WALTHER等: ""An optimized measurement chamber for cantilever array measurements in liquid incorporating an automated sample handling system"", 《EPJ TECHNIQUES AND INSTRUMENTATION》 * |
| 牛晓燕: ""微悬臂梁阵列传感系统设计与实现"", 《中国优秀硕士学位论文全文数据库》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109827904A (en) * | 2019-03-19 | 2019-05-31 | 安徽理工大学 | A kind of reaction pool device based on micro-cantilever beam sensor |
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Application publication date: 20190705 |