CN109957592A - A kind of enzymatic isolation method preparation soybean peptide - Google Patents
A kind of enzymatic isolation method preparation soybean peptide Download PDFInfo
- Publication number
- CN109957592A CN109957592A CN201711334258.5A CN201711334258A CN109957592A CN 109957592 A CN109957592 A CN 109957592A CN 201711334258 A CN201711334258 A CN 201711334258A CN 109957592 A CN109957592 A CN 109957592A
- Authority
- CN
- China
- Prior art keywords
- soybean peptide
- isolation method
- temperature
- enzymatic isolation
- ultrafiltration
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/34—Extraction; Separation; Purification by filtration, ultrafiltration or reverse osmosis
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Medicinal Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- General Chemical & Material Sciences (AREA)
- Water Supply & Treatment (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The present invention relates to a kind of extracting method of effective component of chinese medicine, in particular to a kind of enzymatic isolation method prepares soybean peptide, comprising the following steps: (1) is uniformly mixed soybean protein and water in the ratio of 1:5 ~ 7；(2) protease is added, adjusts pH between 6.5 ~ 7.5, temperature is 35 ~ 40 DEG C, digests 24 ~ 36h；(3) high temperature enzyme deactivation 25 ~ 35 minutes under the conditions of 75 ~ 85 DEG C, cool to room temperature, are filtered；(4) ultrafiltration is carried out with the ultrafiltration membrane that molecular cut off is 8000 ~ 10000；(5) decoloration taste removal；(6) dry.Temperature and the pH control digested in the present invention is suitable for that obtained soybean peptide yield is higher；Using hyperfiltration technique, make enzymolysis product separation thoroughly, high income, soybean peptide molecular weight obtained is small, bioactivity is high, it is easier to absorption of human body；Entire technical conditions are mild, environmentally friendly.
Description
Technical field
The present invention relates to the preparation method of soybean peptide, in particular to a kind of enzymatic isolation method prepares soybean peptide.
Background technique
Soybean peptide is by the soybean protein of macromolecular, using biotechnology enzymatic hydrolysis at being made of 2 to 10 amino acid
Small molecule segment.Soybean peptide is rich in 22 kinds of amino acid, wherein the essential amino acid that cannot be synthesized comprising 9 kinds of human bodies.Soybean peptide is
The protein of small molecule is very easy to be absorbed by the body, and suitable protein digestibility absorbs bad crowd and eats, such as person in middle and old age
People, post-operative recovery patient, tumour and chemicotherapy patient, gastrointestinal function gastrointestinal disease patient etc..In addition, soybean peptide also there is raising to exempt from
Epidemic disease power, relieves fatigue, lower hyperlipidemia, hypertension, hyperglycemia and other effects enhancing physical strength.With zymetology industrial expansion, enzymatic hydrolysis soybean protein gradually at
For the mainstream of research.It is generally all relatively milder that enzyme process prepares the process conditions of soya-bean polypeptides, but because of the difference of enzyme physicochemical property,
Its reaction condition must be limited within the scope of some optimum temperature and pH, and otherwise enzyme will inactivate, and when different enzyme hydrolysis
Action site and the mechanism of action are also different, therefore control temperature and pH are the important steps of enzymatic isolation method preparation soybean peptide.
Summary of the invention
The present invention overcomes deficiencies in the prior art, provide a kind of enzymatic isolation method preparation soybean peptide.
In order to solve the above-mentioned technical problem, the present invention is achieved by the following technical solutions: a kind of enzymatic isolation method preparation is big
Beans peptide, comprising the following steps: (1) be uniformly mixed soybean protein and water in the ratio of 1:5 ~ 7；(2) protease is added, adjusts pH
Between 6.5 ~ 7.5, temperature is 35 ~ 40 DEG C, digests 24 ~ 36h；(3) high temperature enzyme deactivation 25 ~ 35 minutes under the conditions of 75 ~ 85 DEG C, put
It is cool to room temperature, be filtered；(4) ultrafiltration is carried out with the ultrafiltration membrane that molecular cut off is 8000 ~ 10000；(5) decoloration taste removal；(6)
It is dry.
Preferably, the ratio of vegetable protein and water is 1:6 in the step (1).
Preferably, pH is 7 in the step (2), and temperature is 37 DEG C, enzymolysis time 30h.
Preferably, enzyme-removal temperature is 80 DEG C in the step (3), and the enzyme deactivation time is 30 minutes.
Preferably, the molecular cut off of ultrafiltration membrane is 9000 in the step (4).
Preferably, in the step (5) that the filtrate after ultrafiltration is big to pass through by the elution flow rate of 3ml/min
Hole resin decolorization taste removal, obtains soybean peptide clear liquid.
Preferably, dry use is freeze-dried or is spray-dried.
Advantageous effect of the invention is: temperature and the pH control digested in the present invention is suitable for that obtained soybean peptide yield is higher；
Using hyperfiltration technique, make enzymolysis product separation thoroughly, high income, soybean peptide molecular weight obtained is small, bioactivity is high, it is easier to
Absorption of human body；Entire technical conditions are mild, environmentally friendly.
Specific embodiment
Present invention is further described in detail With reference to embodiment:
Embodiment 1
(1) soybean protein 500g is uniformly mixed with water 3000g；
(2) protease is added, adjusting pH is 7, and temperature is 37 DEG C, digests 30h；
(3) high temperature enzyme deactivation 30 minutes under the conditions of 80 DEG C, cool to room temperature, are filtered；
(4) ultrafiltration is carried out with the ultrafiltration membrane that molecular cut off is 9000；
(5) by the filtrate after ultrafiltration, by macroreticular resin decoloration taste removal, it is clear to obtain soybean peptide by the elution flow rate of 3ml/min
Liquid；
(6) the soybean peptide clear liquid after ultrafiltration is freeze-dried, obtains soy peptide powder.
Embodiment 2
Final step is using spray drying unlike the first embodiment, the present embodiment under 0.07mpa pressure condition into
Row is spray-dried, in drying, 125 DEG C of inlet temperature, and 101 DEG C of temperature in drier, 75 DEG C of outlet temperature
Technical characteristic of the present invention without description can realize that details are not described herein, certainly, above-mentioned by or using the prior art
Specific embodiment is not limitation of the present invention, and the present invention is also not limited to above-mentioned specific embodiment, this technology neck
The variations, modifications, additions or substitutions that the those of ordinary skill in domain is made within the essential scope of the present invention should also fall into this hair
In bright protection scope.
Claims (7)
1. a kind of enzymatic isolation method prepares soybean peptide, which comprises the following steps: (1) by soybean protein and water by 1:5 ~ 7
Ratio is uniformly mixed；(2) protease is added, adjusts pH between 6.5 ~ 7.5, temperature is 35 ~ 40 DEG C, digests 24 ~ 36h；(3) exist
High temperature enzyme deactivation 25 ~ 35 minutes under the conditions of 75 ~ 85 DEG C, cool to room temperature, are filtered；(4) with molecular cut off be 8000 ~
10000 ultrafiltration membrane carries out ultrafiltration；(5) decoloration taste removal；(6) dry.
2. enzymatic isolation method according to claim 1 prepares soybean peptide, it is characterised in that: in the step (1) vegetable protein with
The ratio of water is 1:6.
3. enzymatic isolation method according to claim 1 prepares soybean peptide, it is characterised in that: pH is 7 in the step (2), temperature
It is 37 DEG C, enzymolysis time 30h.
4. enzymatic isolation method according to claim 1 prepares soybean peptide, it is characterised in that: enzyme-removal temperature is in the step (3)
80 DEG C, the enzyme deactivation time is 30 minutes.
5. enzymatic isolation method according to claim 1 prepares soybean peptide, it is characterised in that: ultrafiltration membrane cuts in the step (4)
Staying molecular weight is 9000.
6. enzymatic isolation method according to claim 1 prepares soybean peptide, it is characterised in that: will be after ultrafiltration in the step (5)
Filtrate is, by macroreticular resin decoloration taste removal, to obtain soybean peptide clear liquid by the elution flow rate of 3ml/min.
7. enzymatic isolation method according to claim 1 prepares soybean peptide, it is characterised in that: dry use is freeze-dried or sprays
Mist is dry.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711334258.5A CN109957592A (en) | 2017-12-14 | 2017-12-14 | A kind of enzymatic isolation method preparation soybean peptide |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711334258.5A CN109957592A (en) | 2017-12-14 | 2017-12-14 | A kind of enzymatic isolation method preparation soybean peptide |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109957592A true CN109957592A (en) | 2019-07-02 |
Family
ID=67017591
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711334258.5A Pending CN109957592A (en) | 2017-12-14 | 2017-12-14 | A kind of enzymatic isolation method preparation soybean peptide |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109957592A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111253468A (en) * | 2020-01-22 | 2020-06-09 | 广州大学 | Zinc ion complex peptide and complex and application thereof |
| CN111789185A (en) * | 2020-06-29 | 2020-10-20 | 陕西沐亿康美生物科技有限公司 | Method for preparing high-purity soybean peptide powder by enzymolysis |
| CN111848735A (en) * | 2020-07-09 | 2020-10-30 | 中国科学院华南植物园 | Immunoregulation active peptide and preparation method and application thereof |
| CN112239773A (en) * | 2020-11-06 | 2021-01-19 | 汕头市培芝食品有限公司 | Preparation method for preparing peptide molecules by using enzymolysis technology |
-
2017
- 2017-12-14 CN CN201711334258.5A patent/CN109957592A/en active Pending
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111253468A (en) * | 2020-01-22 | 2020-06-09 | 广州大学 | Zinc ion complex peptide and complex and application thereof |
| CN111253468B (en) * | 2020-01-22 | 2021-12-07 | 广州大学 | Zinc ion complex peptide and complex and application thereof |
| CN111789185A (en) * | 2020-06-29 | 2020-10-20 | 陕西沐亿康美生物科技有限公司 | Method for preparing high-purity soybean peptide powder by enzymolysis |
| CN111848735A (en) * | 2020-07-09 | 2020-10-30 | 中国科学院华南植物园 | Immunoregulation active peptide and preparation method and application thereof |
| CN111848735B (en) * | 2020-07-09 | 2022-03-04 | 中国科学院华南植物园 | Immunoregulation active peptide and preparation method and application thereof |
| CN112239773A (en) * | 2020-11-06 | 2021-01-19 | 汕头市培芝食品有限公司 | Preparation method for preparing peptide molecules by using enzymolysis technology |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN109957592A (en) | A kind of enzymatic isolation method preparation soybean peptide | |
| CN108157583B (en) | A kind of highly dissoluble soya-bean polypeptides and its preparation process and application | |
| CN108823273B (en) | Peony seed meal polypeptide with antioxidant activity and preparation method and application thereof | |
| CN102178028B (en) | Method for producing soybean peptide | |
| EP2288716B1 (en) | Method for producing corn gluten hydrolysate and corn gluten hydrolysate using the same | |
| CN110257459B (en) | Bird's nest small molecular peptide and instant peptide powder thereof | |
| CN103305576B (en) | Process for preparing corn peptides by taking corn starch sugar residues as raw materials | |
| CN108486202B (en) | Moringa oleifera hydrolysate and preparation method and application thereof | |
| CN108753894A (en) | A kind of extracting method of American Ginseng peptide | |
| CN101787387B (en) | Acid-resistant no-bitterness soybean oligopeptide and production method and application thereof | |
| CN110734948A (en) | extraction device and process for extracting selenium polypeptide from soybeans | |
| CN104095243A (en) | Preparation technology for enzymolysis bioactive peptide nanometer selenium | |
| CN109825545A (en) | The preparation method of one seed oyster oligopeptide freeze-dried powder | |
| CN106916871A (en) | A kind of bionic enzymatic prepares the production method of freshwater mussel meat small peptide | |
| CN108913741A (en) | A method of using enzymatic isolation method from pilose antler extraction purification pilose antler active oligopeptides, chondroitin sulfate | |
| CN108165583A (en) | Matrimony vine enzymatic hydrolysis and fermentation liquid and preparation process and the application in cosmetics | |
| CN107114793A (en) | It is a kind of to comprehensively utilize the method that sturgeon bone prepares calcium and chondroitin sulfate | |
| CN109486889A (en) | A kind of moringa seeds micromolecule polypeptide and its preparation process | |
| CN107385003B (en) | Preparation process of soybean peptide powder | |
| CN106858236A (en) | A kind of method that aqueous enzymatic method Soy hydrolysate prepares polypeptide compound beverage | |
| CN109957593A (en) | A kind of preparation method of soy peptide powder | |
| CN110128391A (en) | Method for extracting cationic anthocyanin from lonicera edulis | |
| CN103627766A (en) | Preparation method for silky fowl bioactive peptide powder | |
| CN105907826B (en) | Clean preparation method of plant polypeptide/protein | |
| CN110229857B (en) | Bird's nest peptide and preparation method of depolymerized coupling enzymolysis thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20190702 |
|
| WD01 | Invention patent application deemed withdrawn after publication |