CN109954006A - A kind of single-composition Chinese medicine preparation and its preparation method and application - Google Patents
A kind of single-composition Chinese medicine preparation and its preparation method and application Download PDFInfo
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Abstract
The present invention provides a kind of single-composition Chinese medicine preparations and its preparation method and application, which is characterized in that the cmm of simple prescription is prepared using the cicada fungus effective component of artificial culture as primary raw material.Cmm of simple prescription provided by the invention has apparent treatment and prevention effect to the generation and development of chronic renal failure.Animal experiments show that cmm of simple prescription provided by the invention can improve renal function, reduction serum creatinine and urea nitrogen levels, the deposition for improving hemoglobin, reducing ECM, mitigate renal tubular-interstitial injury, delays kidney region fibrosis；The folk prescription safely and effectively, without obvious toxic-side effects.
Description
Technical field
The invention belongs to Chinese medicines and natural medicine technical field, and in particular to it is a kind of prevention and treatment chronic renal failure and its
The single-composition Chinese medicine preparation and its preparation method and application of his chronic kidney disease.
Background technique
Chronic renal failure (CRF) is chronic renal disease and involves chronic kidney function caused by renal system disease
It can decline, and syndrome composed by the various clinical symptoms generated therefrom and metabolic disorder.CRF is a kind of serious harm
The difficult and complicated illness of patient body health or even life-threatening.A large amount of clinical and experimental studies confirmations, the chronic kidney disease of different pathogeny
After kidney is by a degree of destruction, although its underlying pathology has stopped, renal function can still continue to decline.Cause
How this carries out early prevention and treatment to CRF, and delay the disease progression of CRF, and reducing uremic disease incidence just seems especially heavy
It wants.
Recent study discovery, natural medicinal entomogenous fungi cicada fungus have the synthesis for reducing extracellular matrix, delay kidney small
Ball hardening improves the effect of renal function, but natural cicada fungus is faced with scarcity of resources, and expensive, the production cycle is long, low output
Problem.It was discovered by researchers that the artificial culture object of cicada fungus not only have effects that with natural cicada fungus similar ingredient and, especially exist
It treats in chronic renal failure, it is same that there are reduction serum creatinine and urea nitrogen levels, improve renal function, inhibits proliferation of mesangial cells
Increase with matrix, delays the hardening of glomerulus to be in progress, while also can solve the scarcity of resources of natural cicada fungus, low output, quality
The problems such as unstable.
Summary of the invention
It is an object of the invention to overcome the deficiencies of existing technologies, a kind of single-composition Chinese medicine preparation is provided.The Chinese materia medica preparation pair
Chronic renal failure and the generation of other chronic kidney diseases and development have apparent treatment and prevention effect, can reduce serum xanthine
Oxidase active.
To achieve the above object, the present invention adopts the following technical scheme that:
A kind of single-composition Chinese medicine preparation contains the artificial cultured products of cicada fungus in the single-composition Chinese medicine preparation.
Preferably, the artificial cultured products of cicada fungus comprise the following steps:
1) prepared by strain: preparing and level-one kind preparation process including inclined-plane kind；
Wherein, inclined-plane kind prepares the Paecilomyces cicadae bacterial strain after including the following steps: to separate unit cell and is transplanted to PSA medium slant
In test tube, cultivated 5~7 days at 22 DEG C~25 DEG C, selecting growing way, good kind saves backup；
Prepared by level-one kind includes the following steps: to access inclined-plane kind in potato sucrose culture solution, at 22 DEG C~25 DEG C, oscillation frequency
Rate is cultivated 3~7 days under conditions of being 130~150r/min, until spare as level-one kind after occurring a large amount of mycelium pellets in conical flask；
2) it is inoculated with: level-one kind obtained in step 1) is linked into solid medium；
3) ferment: the culture box after inoculation moves in culturing room, under conditions of 20 DEG C~25 DEG C, relative humidity 60%~80%
Culture 30 days~50 days；
4) harvesting of coremium: when the coremium height of culture has a small amount of conidium to occur up to 9cm or so, surface
Harvesting.
Preferably, in step 1), contain following component (containing in every 1000ml culture medium) in the PSA culture medium: horse
Bell potato 200g, sucrose 20g, agar 20g, preparation method are as follows: peeled potatoes, which are diced, to be placed in container adds water 1000ml, boils
20min~30min is boiled, sucrose and agar is added in filtering, supplies water to 1000ml, then heating for dissolving is dispensed to test tube,
Loading amount is long 1/5~1/4 of test tube, and tampon, is placed in autoclave the 30min that sterilizes under 0.15MPa pressure beyond the Great Wall, while hot by test tube
It is placed on skewback cooling.
Preferably, in step 1), (contain in every 1000ml culture solution in the potato sucrose culture solution containing following component
Have): potato 200g, sucrose 20g；Preparation method are as follows: peeled potatoes, which are diced, to be placed in container adds water 1000ml, boils
Sucrose is added in 15min~30min, filtering, supplies water to 1000ml, dissolves by heating, then dispense to conical flask, loading amount is
The 20%~30% of conical flask capacity, tampon beyond the Great Wall, then wrapped up with brown paper, it is placed in autoclave and sterilizes under 0.15MPa pressure
30min, taking-up are cooled to 20 DEG C or less.
Preferably, in step 2), the step of the inoculation are as follows: when the temperature of the solid medium in culture box is cooled to 20
DEG C or less after, be aseptically inoculated with, according to 3~5% inoculum concentration by level-one kind access culture box in.
Preferably, in step 2), the raw material components of the solid medium include:
42~46 parts by weight of matrix；
2~10 parts by weight of bagasse；
0.1~0.5 parts by weight of conch meal；
2~5 parts by weight of dried silkworm chrysalis meal；
0.1~0.5 parts by weight of potassium nitrate；
44~56 parts by weight of water；
Wherein, the matrix is selected from one of following combination: the mixture of corn flour, wheat bran and sucrose；Wheat；Barley；Grain
With the mixture of sucrose；The mixture of rice and sucrose；The mixture of sorghum and sucrose.
Preferably, in the mixture of the corn flour, wheat bran and sucrose, with the mixing of the corn flour, wheat bran and sucrose
The total weight of object, the weight percent of corn flour, wheat bran and sucrose be respectively as follows: corn flour 43%~55%, wheat bran 44%~
55%, sucrose 1%~2%.
Preferably, in the mixture of the grain and sucrose, with the total weight of the grain and the mixture of sucrose, grain and sugarcane
The weight percent of sugar is respectively as follows: grain 98%~99%, sucrose 1%~2%.
Preferably, in the mixture of the rice and sucrose, with the total weight of the rice and the mixture of sucrose, greatly
The weight percent of rice and sucrose is respectively as follows: rice 98%~99%, sucrose 1%~2%.
Preferably, high with the total weight of the sorghum and the mixture of sucrose in the mixture of the sorghum and sucrose
The weight percent of fine strain of millet and sucrose is respectively as follows: sorghum 98%~99%, sucrose 1%~2%.
Preferably, the solid medium the preparation method comprises the following steps: first by corn flour, wheat bran or wheat, barley, grain, rice,
Sorghum is cooked, and sucrose, potassium nitrate are dissolved in water, then with bagasse, conch meal, dried silkworm chrysalis meal is proportionally uniformly mixed.
Preferably, also contain peptone (fish meal) in the solid medium, and the parts by weight of the peptone (fish meal)
For 0.5 parts by weight.
Preferably, in step 2), the carbon-nitrogen ratio of the solid medium is 80~98.
Preferably, in step 3), daylighting is required in solid fermentation later period coremium growth phase, temperature is compared with mycelial growth
It slightly lower 2 DEG C of temperature, needs to maintain the circulation of air in incubation.
Preferably, in step 4), the specific steps of the harvesting are as follows: remove breathable sealing film, taken culture with tool
Coremium is separated out, and drying classification is vacuum-packed after dry with bag film, mycoplasma is placed in -20 DEG C of storages.
The artificial cultured products of heretofore described cicada fungus are by coremium, conidium, mycelium and culture medium residue
The mixture of matter.
It is further preferred that the artificial cultured products of cicada fungus refer to using Paecilomyces cicadae bacterial strain CGMCC No.3453
The artificial cultured products that [Paecilomyces cicadae] is obtained after above-mentioned solid culture, the bacterial strain is in November, 2009
Preservation was registered at China Committee for Culture Collection of Microorganisms's common micro-organisms center (abbreviation CGMCC) in 18th, deposit number is
CGMCC No.3453。
The present invention also provides the preparation method of the single-composition Chinese medicine preparation, the preparation method is in following three kinds
It is a kind of:
1) it crushes: will directly be crushed after the artificial cultured products drying of cicada fungus, cross 120 meshes and be made；
2) solvent extraction；
3) the cicada fungus artificial culture object is added water to cook to (institute's water consumption be cicada fungus artificial culture object weight 3-6 times), is filtered,
It is concentrated into right amount, is drying to obtain xeraphium.
Preferably, the solvent extraction is to be extracted using organic solvent, or mention for water, or be the mixing of organic solvent and water
Solution extracts, and further preferably water mentions.
Preferably, the organic solvent is selected from one of ethyl alcohol, acetone and petroleum ether or a variety of mixtures；It is described to have
The mixed solution of solvent and water is the extract of ethanol water.
Preferably, the decoction number is 2~3 times, often in the preparation method that the cicada fungus artificial culture object adds water to cook
Secondary decocting time is 0.5~1 hour.
Preferably, in the preparation method that the cicada fungus artificial culture object adds water to cook, the decocting liquid obtained after the decoction is first
80 meshes are crossed, removal of impurities is filtered.
Preferably, in the preparation method that the cicada fungus artificial culture object adds water to cook, the concentration is decompression film concentration,
The drying is spray drying.
The third aspect of the invention provides a kind of Chinese medicine composition, which contains safe and effective amount
Above-mentioned folk prescription Chinese medicine and pharmaceutically acceptable carrier, the weight percentage of the folk prescription Chinese medicine are 50%-99%, preferably
62%-80%.
Pharmaceutically acceptable carrier is various pharmaceutically common auxiliary materials and/or excipient, including but not limited to sugar
Class (such as lactose, dextrose and saccharose), starch (such as cornstarch and potato starch), cellulose and its derivates (such as carboxymethyl
Sodium cellulosate, ethyl cellulose and methylcellulose), tragacanth gum powder, malt, gelatin, talcum, solid lubricant is (such as tristearin
Acid and magnesium stearate), calcium sulfate, vegetable oil is polynary such as peanut oil, cottonseed oil, sesame oil, olive oil, corn oil and cupu oil
Alcohol (such as propylene glycol, glycerol, D-sorbite, mannitol and polyethylene glycol), alginic acid, emulsifier (such as Tween), wetting agent
(such as NaLS), colorant, flavoring agent, tablet agent, stabilizer, antioxidant, preservative, apirogen water, isotonic salt
Solution and phosphate buffer etc.；These substances be used to help as needed formula stability or help to improve activity or it
Biological effectiveness or acceptable mouthfeel or smell are generated in the case where oral.
Any conventional dosage form can be made in single-composition Chinese medicine preparation of the invention according to the universal method in pharmacy,
Including oral preparation.Preferably tablet, pulvis agent, granule, pill, pulvis, syrup, most preferably pulvis.
Single-composition Chinese medicine preparation pulvis of the invention, preferable taking dose are 2-15g/ days, and optimal dosage is 5-10g/
It.
The fourth aspect of the invention provides the purposes of above-mentioned single-composition Chinese medicine preparation, i.e., the folk prescription Chinese medicine is used to prepare
The drug for the treatment of and prevention chronic renal failure and other chronic kidney diseases.Other described chronic kidney diseases refer to be damaged with renal tubulointerstitial
Nephrosis based on evil, including uric acid nephropathy, chronic pyelonephritis, drug-induced renal impairment etc..
The fifth aspect of the invention provides the purposes of above-mentioned single-composition Chinese medicine preparation, i.e., the folk prescription Chinese medicine is used to prepare
Treatment and prevention can reduce the drug of serum creatinine and urea nitrogen.
Term explanation: heretofore described cicada fungus artificial culture object, that is, Paecilomyces cicadae, Latin are entitled
Paecilomycescicadae。
Animal experiments show that folk prescription Chinese medicine provided by the invention can improve renal function, improve hemoglobin, reduce ECM
Deposition, reduce proliferation of mesangial cells, mitigate Renal tissues damage, delay kidney region fibrosis；Its drug activity and natural cicada fungus
Effect is compared to similar or more preferable, and safety is without obvious toxic-side effects.
In the present invention, the single-composition Chinese medicine preparation is made of the raw material, refers to and is directly crushed or mentioned by the raw material
It takes and is made.
Specific embodiment
Present invention will be further explained below with reference to specific examples.It should be understood that these embodiments are merely to illustrate the present invention
Rather than it limits the scope of the invention.
The artificial culture of 1 single-composition Chinese medicine preparation of embodiment
Picking Paecilomyces cicadae strain accesses in PSA culture solution, 22 DEG C -25 DEG C, spare after cultivating 3-5 days under conditions of 140rpm；
Seed is accessed in solid medium, 20 DEG C -25 DEG C according to 3-5% ratio, cultivates 30d- under relative air humidity 60%-80%
50d, later period coremium growth phase require daylighting, 2 DEG C slightly lower compared with the mycelial growth stage of temperature, need to open in due course daily
Window ventilation keeps culture room air fresh.
The PSA Medium Proportion that present embodiment uses is every 1000ml culture medium: potato 200g, sucrose 20g, agar
20g。
The proportion of solid medium described in present embodiment is 46 parts by weight of matrix, 5 parts by weight of bagasse, conch meal 0.4
Parts by weight, 2.5 parts by weight of dried silkworm chrysalis meal, 0.1 parts by weight of potassium nitrate, water are 44 parts by weight；Its mesostroma is wheat.Preparation method
Are as follows: first wheat is cooked, sucrose, potassium nitrate are dissolved in water, then with bagasse, conch meal, dried silkworm chrysalis meal proportionally mixes
Uniformly.
One of the preparation method of 2 single-composition Chinese medicine preparation of embodiment.
By the fresh cicada fungus artificial culture object of harvesting, 70 DEG C of dry 10h are crushed by pulverizer, cross 120 mesh screens, i.e.,
Obtain xeraphium.
The two of the preparation method of 3 single-composition Chinese medicine preparation of embodiment.
Water is added to decoct altogether 2 times the cicada fungus artificial culture object, 0.5 hour every time, decocting liquid removed impurity elimination using vacuum filtration
Matter, obtains filtrate, and decompression film is concentrated into right amount, is spray-dried up to xeraphium.
Renal function of the single-composition Chinese medicine preparation of 4 embodiment 2 of embodiment to 5/6 rat remnant model.
The present embodiment data quoted from: Zhu Rong, Chen Yi-ping, Deng Yueyi, Wang Lin solid culture CSM treated delay glomerulus hard
Change the experimental study of Function and its mechanisms, Chinese combination of Chinese tradiational and Western medicine nephrosis magazine, 2005,6 (2): 70-74.
Positive control drug: Cozaar, the production of Hangzhou Mo Shadong pharmaceutical Co. Ltd；Natural cicada fungus, the place of production are Zhejiang.
Experimental animal: SPF grades male SD rat 70, at 2 monthly ages, 200 ± 10g of weight is tested by Shanghai Univ. of Traditional Chinese Medicine
Animal center provides.
Grouping and modeling: SD rat adaptable fed is after 1 week, is randomly divided into 2 groups: blank group (sham-operation group) 8 and real
Test group (operation group) 62.Experimental rat model: the 1st operation, operation group rat is established using 5/6 kidney major part cutting method
Left kidney always cuts off 2/3 that cortex renis weight accounts for left kidney weight；The 2nd operation is carried out after 7d, right kidney is cut entirely.Two operations are for cutting
Except the 5/6 of two kidney total weights.Sham-operation group SD rat separates kidney peplos, but the not nephrectomy using same step exposure kidney.
Operation group animal survives 54, sham-operation group all survivals 8.2 weeks after the 2nd operation, all animal row inner canthus sound
The blood sampling of arteries and veins clump carries out serum creatinine (Scr) detection.And by 54 operation group rats, it is divided into 5 groups according to Scr stratified random: model pair
According to group (model group) 12, Cozaar group 10, natural cicada fungus group 11, artificial cicada fungus large dosage group (A group) 10, artificial cicada
11, flower small dose group (B group).Cozaar group, natural cicada fungus group, artificial cicada fungus large dosage group (A group), artificial cicada fungus are low dose of
Group (B group) rat 4 weeks beginning medicine feeds, treatment time 42d after the 2nd operation.Survival of rats 44 at the end of experiment, artificial hand
Art group, each 8 of artificial cicada fungus large dosage group (A group), model group, Cozaar group, natural cicada fungus group, artificial cicada fungus small dose group (B
Group) it is 7 each.
Dosage: natural cicada fungus group, artificial cicada fungus large dosage group (A group) rat press 4gkg-1·d-1Dosage, manually
Cicada fungus small dose group (B group) presses 2gkg-1·d-1Dosage is added in feed and is administered, and per daily physiological saline stomach-filling 1 time；Section
Plain subgroup rat uses administration by gavage, by 30mgkg-1·d-1Dosage is dissolved in physiological saline, daily stomach-filling 1 time.All rats
It is grouped sub-cage rearing, 4-5 rat of every cage, whole rat free waters, feed commercially available blocky-shaped particle normal diet during experiment.
Test equipment and method: the Scr, urea nitrogen (BUN), albumin (Alb) of all rats are detected at the end of experiment；It receives
Collect urine for 24 hours, measures quantity of proteinuria.The above index is measured using 7170 type automatic biochemistry analyzer of Hitachi.
Glomerulosclerosis is checked with HE dyeing and PAS dyeing renal pathology piece, wherein glomerulosclerosis is defined as capillary
Lumen of vessels collapses and/or hyalinization.Glomerulosclerosis degree is only assessed using the semi-quantitative method of Rajj etc..Light microscopic (×
200) under the visual field, every slice at least observes 40 glomerulus, is divided into 0-+++ according to glomerulus ratio shared by glomerulosclerosis stove
+, 0 indicates glomerulus without hardening, and the 25% of 1 glomerulus of+expression is impaired, ++ indicate that 26%-50% is impaired, +++ indicate 51%-
75% is impaired, ++++indicate that 76%-100% is impaired.Then glomerulosclerosis index (GSI) is calculated.
Data statistics is carried out using 11.0 for Windows of SPSS Version, is indicated with mean ± standard deviation, group
Between compare using One-Way ANOVA analyze, statistical significance level be P < 0.05.
Test result: as a result, it has been found that, Scr, BUN value of rat, each treatment group significantly reduce (P < 0.05) compared with model group,
Artificial cicada fungus each group (large dosage of group, small dose group) no difference of science of statistics (P > 0.05) compared with natural cicada fungus, the blood of rat is white
Protein value, sham-operation group, Cozaar group, natural cicada fungus group, artificial cicada fungus large dosage group (A group) significantly increase (P < 0.05),
And artificial cicada fungus large dosage group (A group) no difference of science of statistics (P > 0.05) compared with natural cicada fungus group and sham-operation group；Rat
Urine albumen amount for 24 hours, each group obviously increase (P < 0.05) compared with sham-operation group, but Cozaar group, natural cicada fungus group, artificial
Cicada fungus large dosage group (A group) then significantly reduces (P < 0.05) compared with model group.
Conventional H E and PAS dyeing, light microscopic observation renal pathology variation: rats in sham-operated group glomerulus blood vessels open
It puts, clear in structure, glomerulus collagen is in thin-line-shaped distribution after PAS dyeing；Severe in model group rats glomerular mesangium territorial matrix
Increase with severe hyperplasia in mesangial cell, part Capillary loops, which are pressurized, to be occluded, hyaloid lesion, and is soaked with interstitial lymphocyte
Moisten, sees protein cast in the renal tubule of part.Each treatment group's glomerulosclerosis degree is more substantially reduced than model group (P < 0.05), according to
Secondary is the natural cicada fungus group of B group > A group > Cozaar group >, and A group, B group compare no difference of science of statistics (P > with natural cicada fungus group
0.05)。
Comparison of the single-composition Chinese medicine preparation and cordyceps mycelia of 5 embodiment 2 of embodiment to renal function
The present embodiment data quoted from: Jin Zhouhui, Chen Yi-ping, Deng Yueyi CSM treated delay the mechanism of action of glomerulosclerosis to grind
Study carefully, Chinese combination of Chinese tradiational and Western medicine nephrosis magazine, 2005,6 (3): 132-136
Tested material: the single-composition Chinese medicine preparation of embodiment 2.
Positive control drug: Cozaar, the production of Hangzhou Mo Shadong pharmaceutical Co. Ltd；Chinese caterpillar fungus hypha is purchased from Jiangxi Province's Jinshuibao
Pharmaceutical Co. Ltd.
Experimental animal: cleaning grade grade male SD rat 56,195.93 ± 20.43g of weight, by Shanghai Univ. of Traditional Chinese Medicine's reality
Animal center offer is provided.
Grouping and modeling: all rat adaptable feds were randomly divided into sham-operation group 8 and modeling group 48 after 1 week.It makes
Mould method: left kidney always cuts off cortex renis weight accounts for left kidney weight 2/3 to modeling group rat for the first time；The 2nd operation is carried out after 2 weeks,
The right kidney of row is cut entirely.Two operations are for cutting off the 5/6 of two kidney total weights.Sham-operation group SD rat exposes kidney using same step,
Separate kidney peplos, but the not nephrectomy.Modeling group rat is randomly divided into model control group, CSM treated group, Cozaar group, worm
Careless mycelia group, every group each 12.Cozaar group, CSM treated group, Chinese caterpillar fungus hypha group rat start to feed after the 2nd operation for 4 weeks
Medicine, treatment time are 6 weeks.
Dosage: CSM treated group and Chinese caterpillar fungus hypha group rat press 30gkg-1·d-1Dosage be made into feed to
Medicine, and per daily physiological saline stomach-filling 1 time, record the dietary amount of rat；Cozaar group rat uses administration by gavage, by 10mg
kg-1·d-1Dosage is dissolved in physiological saline, one time a day stomach-filling.Dead 4 of model group rats in administration process, Cozaar is big
Mouse dead 2, death does not occur for remaining each group rat.
Test equipment and method: all experimentss animal is after treatment 6 weeks, 1d whole rat limosis before putting to death, next day blood sampling,
Detect the red blood cell count(RBC) (RBC) of all rats, hemochrome (Hb), hematocrit (HCT), renal function (BUN, Scr), blood lipid
(TG, TC), albumin (Alb) check.The above index is measured using 7170 type automatic biochemistry analyzer of Hitachi.
After experimental animal is drawn blood, left kidney, row HE dyeing are taken out rapidly.Using medical pathologies image processing system to kidney
The pathological lesion degree of bead is analyzed.It is small that every slice continuously randomly selects similar 25 kidneys of area under high power lens
Ball absorb image, input Computer digital image analysis in, in system screen, calculate each Glomerular lesions involve area and
The ratio of the glomerulus gross area of selection, the fiducial value for the glomerulus pathology extent of damage for taking its mean value to be sliced as every.
Data statistics is carried out using 11.0 for Windows of SPSS Version, is indicated with mean ± standard deviation, group
Between compare using One-Way ANOVA analyze, statistical significance level be P < 0.05.
Test result: as a result, it has been found that, CSM treated group, Chinese caterpillar fungus hypha group and Cozaar group red blood cell count(RBC), hemoglobin,
Hematocrit is above model group rats, there is statistical difference (P < 0.05 or P < 0.01)；Chinese caterpillar fungus hypha group and section's element
The counting of subgroup Rat Erythrocytes, hemoglobin, hematocrit are lower than CSM treated group rat (P < 0.05).Model group rats
BUN, Scr are apparently higher than CSM treated group (P < 0.01), Chinese caterpillar fungus hypha group and Cozaar group Scr and are above CSM treated group (P
< 0.05), Cozaar group BUN be higher than CSM treated group (P < 0.05).CSM treated group rat plasma albumin is apparently higher than mould
Type group rat (P < 0.01).CSM treated group rat plasma cholesterol is lower than model group rats, there is statistical difference (P <
0.05)。
Model group rats pathology damage is compared with CSM treated group, Chinese caterpillar fungus hypha combination Cozaar group is serious, mesangial cell proliferation
Obviously, mesentery is broadening, and segment hardening or even ground-glass-like lesion and whole world hardening occurs in part glomerulus, has statistics poor
Different (P < 0.05 or P < 0.01)；CSM treated group is compared with Chinese caterpillar fungus hypha combines Cozaar group rat, renal pathology damage
Mitigate obviously, there is statistical difference (P < 0.01).
Claims (4)
1. a kind of single-composition Chinese medicine preparation, the artificial cultured products of cicada fungus are contained in the single-composition Chinese medicine preparation, the cicada fungus is manually cultivated
Product comprises the following steps:
1) prepared by strain: preparing and level-one kind preparation process including inclined-plane kind；
2) it is inoculated with: level-one kind obtained in step 1) is linked into solid medium；
3) ferment: the culture box after inoculation moves in culturing room, trains under conditions of 20 DEG C~25 DEG C, relative humidity 60%~80%
It supports 30 days~50 days；
4) it harvests；
In step 2, the raw material components of the solid medium include:
42~46 parts by weight of matrix；
2~10 parts by weight of bagasse；
0.1~0.5 parts by weight of conch meal；
2~5 parts by weight of dried silkworm chrysalis meal；
0.1~0.5 parts by weight of potassium nitrate；
44~56 parts by weight of water；
Wherein, the matrix is selected from one of following combination: the mixture of corn flour, wheat bran and sucrose；Wheat；Barley；Grain
With the mixture of sucrose；The mixture of rice and sucrose；The mixture of sorghum and sucrose；The corn flour, wheat bran and sucrose
In mixture, with the total weight of the mixture of the corn flour, wheat bran and sucrose, the weight hundred of corn flour, wheat bran and sucrose
Ratio is divided to be respectively as follows: corn flour 43%~55%, wheat bran 44%~55%, sucrose 1%~2%；Also contain albumen in the solid medium
Peptone, and the parts by weight of the peptone are 0.5 parts by weight；The artificial cultured products of cicada fungus refer to using Paecilomyces cicadae bacterial strain
The artificial cultured products that CGMCC No.3453 is obtained after above-mentioned solid culture.
2. the preparation method of single-composition Chinese medicine preparation described in claim 1, the preparation method is selected from one of following three kinds:
1) it crushes: will directly be crushed after the artificial cultured products drying of cicada fungus, cross 120 meshes and be made；
2) solvent extraction；
3) the cicada fungus artificial culture object is added water to cook, is filtered, be concentrated into right amount, be drying to obtain xeraphium.
3. a kind of Chinese medicine composition, the Chinese medicine composition contains the folk prescription Chinese medicine system described in claim 1 of safe and effective amount
Agent and pharmaceutically acceptable carrier, wherein the weight percentage of the single-composition Chinese medicine preparation is 50%-99%.
4. single-composition Chinese medicine preparation described in claim 1 is in preparation treatment and prevention chronic renal failure and the drug of other chronic kidney diseases
The purposes of aspect.
Priority Applications (1)
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| WO2021093425A1 (en) * | 2019-11-15 | 2021-05-20 | 滨州医学院 | Root of membranous milkvetch-paecilomyces cicadae fermentative bacterium fermentation substance and application thereof |
| US11866692B2 (en) | 2019-11-15 | 2024-01-09 | Binzhou Medical University | Fermentation fungal substance of astragalus membranaceus paecilomyces cicadae and its use |
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