CN109953320A - The preparation method of cactus enzyme - Google Patents
The preparation method of cactus enzyme Download PDFInfo
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- CN109953320A CN109953320A CN201711420840.3A CN201711420840A CN109953320A CN 109953320 A CN109953320 A CN 109953320A CN 201711420840 A CN201711420840 A CN 201711420840A CN 109953320 A CN109953320 A CN 109953320A
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- 241000219357 Cactaceae Species 0.000 title claims abstract description 41
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- 108090000790 Enzymes Proteins 0.000 title claims abstract description 23
- 238000002360 preparation method Methods 0.000 title claims abstract description 20
- 238000000855 fermentation Methods 0.000 claims abstract description 54
- 230000004151 fermentation Effects 0.000 claims abstract description 47
- 235000000346 sugar Nutrition 0.000 claims abstract description 25
- 241000894006 Bacteria Species 0.000 claims abstract description 22
- 239000006041 probiotic Substances 0.000 claims abstract description 20
- 235000018291 probiotics Nutrition 0.000 claims abstract description 20
- 241000235342 Saccharomycetes Species 0.000 claims abstract description 16
- 240000001046 Lactobacillus acidophilus Species 0.000 claims abstract description 13
- 235000013956 Lactobacillus acidophilus Nutrition 0.000 claims abstract description 13
- 229940039695 lactobacillus acidophilus Drugs 0.000 claims abstract description 13
- 244000063299 Bacillus subtilis Species 0.000 claims abstract description 11
- 235000014469 Bacillus subtilis Nutrition 0.000 claims abstract description 11
- 241000186000 Bifidobacterium Species 0.000 claims abstract description 11
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- 229940072205 lactobacillus plantarum Drugs 0.000 claims abstract description 11
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- 238000011049 filling Methods 0.000 claims abstract description 4
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- 244000199885 Lactobacillus bulgaricus Species 0.000 claims description 9
- 235000013960 Lactobacillus bulgaricus Nutrition 0.000 claims description 9
- 241000194020 Streptococcus thermophilus Species 0.000 claims description 9
- 229940004208 lactobacillus bulgaricus Drugs 0.000 claims description 9
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- 238000003860 storage Methods 0.000 claims description 4
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- 229910017435 S2 In Inorganic materials 0.000 claims 1
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- KDYFGRWQOYBRFD-UHFFFAOYSA-N succinic acid Chemical compound OC(=O)CCC(O)=O KDYFGRWQOYBRFD-UHFFFAOYSA-N 0.000 description 2
- BJEPYKJPYRNKOW-REOHCLBHSA-N (S)-malic acid Chemical compound OC(=O)[C@@H](O)CC(O)=O BJEPYKJPYRNKOW-REOHCLBHSA-N 0.000 description 1
- QTBSBXVTEAMEQO-UHFFFAOYSA-M Acetate Chemical compound CC([O-])=O QTBSBXVTEAMEQO-UHFFFAOYSA-M 0.000 description 1
- 108010059892 Cellulase Proteins 0.000 description 1
- AUNGANRZJHBGPY-UHFFFAOYSA-N D-Lyxoflavin Natural products OCC(O)C(O)C(O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-UHFFFAOYSA-N 0.000 description 1
- 101710088194 Dehydrogenase Proteins 0.000 description 1
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- 102000005548 Hexokinase Human genes 0.000 description 1
- 108700040460 Hexokinases Proteins 0.000 description 1
- 241000186866 Lactobacillus thermophilus Species 0.000 description 1
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 description 1
- 102000004882 Lipase Human genes 0.000 description 1
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- 102000013009 Pyruvate Kinase Human genes 0.000 description 1
- 108020005115 Pyruvate Kinase Proteins 0.000 description 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 1
- AUNGANRZJHBGPY-SCRDCRAPSA-N Riboflavin Chemical compound OC[C@@H](O)[C@@H](O)[C@@H](O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-SCRDCRAPSA-N 0.000 description 1
- 244000269722 Thea sinensis Species 0.000 description 1
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- 238000010521 absorption reaction Methods 0.000 description 1
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- 239000002253 acid Substances 0.000 description 1
- 230000009858 acid secretion Effects 0.000 description 1
- 229930013930 alkaloid Natural products 0.000 description 1
- 150000003797 alkaloid derivatives Chemical class 0.000 description 1
- BJEPYKJPYRNKOW-UHFFFAOYSA-N alpha-hydroxysuccinic acid Natural products OC(=O)C(O)CC(O)=O BJEPYKJPYRNKOW-UHFFFAOYSA-N 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 102000005936 beta-Galactosidase Human genes 0.000 description 1
- 108010005774 beta-Galactosidase Proteins 0.000 description 1
- 229940106157 cellulase Drugs 0.000 description 1
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- 238000005516 engineering process Methods 0.000 description 1
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- 239000012530 fluid Substances 0.000 description 1
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- 150000004676 glycans Chemical class 0.000 description 1
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- 210000000936 intestine Anatomy 0.000 description 1
- 239000008101 lactose Substances 0.000 description 1
- 235000019421 lipase Nutrition 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
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- 239000001384 succinic acid Substances 0.000 description 1
- 150000003648 triterpenes Chemical class 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/125—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives containing carbohydrate syrups; containing sugars; containing sugar alcohols; containing starch hydrolysates
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/135—Bacteria or derivatives thereof, e.g. probiotics
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/14—Yeasts or derivatives thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/113—Acidophilus
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/123—Bulgaricus
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/169—Plantarum
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/21—Streptococcus, lactococcus
- A23V2400/249—Thermophilus
Abstract
The present invention relates to a kind of preparation methods of cactus enzyme, include the following steps: step S1: by cactus peeling, being broken into fourth, be placed in round；Step S2: sugar is added；Step S3: pure water is added；Step S4: mixing probiotics, anaerobic fermentation is added；Step S5: after fermentation in filtered under aseptic conditions；Step S6: it is filling under aseptic condition after initial survey is qualified, it examines, output finished product；Wherein, in the step S4, the probiotics being added includes saccharomycete, bacillus subtilis, lactobacillus plantarum, lactobacillus acidophilus and Bifidobacterium.The preparation method of cactus enzyme provided by the invention shortens zymotechnique, increases finished product pH value, control the miscellaneous bacteria in fermentation process by the selection of fermenting microbe and the control of anaerobic fermentation environment, improves the nutritive value of prepared ferment.
Description
Technical field
The present invention relates to technical field of microbial fermentation, and in particular to a kind of preparation method of cactus enzyme.
Background technique
Lactic acid bacteria is the key that maintain ferment quality flora, and in traditional cactus enzyme production technology, multi-pass is excessively newborn
Sour bacterium realizes the spontaneous fermentation of cactus, however, this method has following many defects: final to ferment in natural fermentation process
It is easy to be mixed into many miscellaneous bacterias in product, influences product quality, also, since traditional natural fermentating process is extensive, it is produced
For finished product acidity mostly 4 hereinafter, can not inhibit miscellaneous bacteria, two kinds of factors cause final product produced to be that quality is produced without guarantee
Product, uncontrollable product.
Accordingly, it is desirable to provide a kind of preparation method of new cactus enzyme.
Summary of the invention
To solve the shortcomings of the prior art, the present invention provides a kind of preparation methods of cactus enzyme, including such as
Lower step:
Step S1: by cactus peeling, it is broken into fourth, is placed in round；
Step S2: sugar is added；
Step S3: pure water is added；
Step S4: mixing probiotics, anaerobic fermentation is added；
Step S5: after fermentation in filtered under aseptic conditions；
Step S6: it is filling under aseptic condition after initial survey is qualified, it examines, output finished product；
Wherein, in the step S4, the probiotics being added include saccharomycete, bacillus subtilis, lactobacillus plantarum,
Lactobacillus acidophilus and Bifidobacterium.
Wherein, in the step S4, the probiotics being added further includes lactobacillus bulgaricus and streptococcus thermophilus.
Wherein, the step S4 includes:
Step S41: being added saccharomycete and bacillus subtilis, and room temperature sealed fermenting keeps one-way exhaust and gentle agitation,
Until being no longer vented；
Step S42: lactobacillus plantarum, lactobacillus acidophilus, Bifidobacterium, lactobacillus bulgaricus and thermophilus is added
Bacterium, room temperature sealed fermenting keep one-way exhaust and gentle agitation, until being no longer vented；
Wherein, in the step S41, the additive amount of probiotics and the volume ratio of cactus fermentation raw material are between 1%-2%；
In the step S42, the additive amount of probiotics and the volume ratio of cactus fermentation raw material are between 2%-4%；
Also, the viable count of the storage liquid for every kind of probiotics being added is between 1-2 × 104CFU/ml。
Wherein, in the step S41, the volume ratio of saccharomycete and bacillus subtilis is between 1:4-1:1；The step
In S42, volume that lactobacillus plantarum, lactobacillus acidophilus, Bifidobacterium, lactobacillus bulgaricus and streptococcus thermophilus are added
Number respectively between: 0.8-1.2 parts, 2-4 parts, 2-4 parts, 1-3 parts and 1-3 parts.
Wherein, in the step S4, the saccharomycete being added is kluyveromyces marxianus bacterium.
Wherein, in the step S1, used round is native terrine.
Wherein, in the step S2, the sugar being added is brown sugar, molasses or maltose.
Wherein, in the step S2, if the sugar being added is brown sugar or molasses, the quality of sugar, cactus and pure water
Proportion is 1:1:1 or 1:3:1；If the sugar being added is maltose, the quality proportioning of sugar, cactus and pure water is 1:1:1.
The preparation method of cactus enzyme provided by the invention passes through the selection of fermenting microbe and the control of anaerobic fermentation environment
System, shortens zymotechnique, increases finished product pH value, control the miscellaneous bacteria in fermentation process, improves prepared ferment
The nutritive value of element.
Specific embodiment
In order to have further understanding to technical solution of the present invention and beneficial effect, the following detailed description of of the invention
Technical solution and its beneficial effect of generation.
The preparation method of cactus enzyme provided by the invention, for the deficiency of existing cactus enzyme zymotechnique,
The various aspects such as selection and proportion from fermentation strain, yeasting and sugar are improved, and the finished product finally prepared is made to have both battalion
The selection for supporting value and safety, especially fermentation strain, highly shortened fermentation period, improves final product quality and finished product
Safety.The present invention has mainly selected saccharomycete, bacillus subtilis, lactobacillus plantarum, lactobacillus acidophilus, Bifidobacterium, guarantor
Add Leah lactobacillus and streptococcus thermophilus as probiotics, the effect of every kind of probiotics during the fermentation approximately as:
One, saccharomycete
Saccharomycete can accelerate anaerobic environment and be formed, and promote anaerobic lactobacter growth；Egg in increase and abundant fermentation liquid
Bai Hanliang；And by improving the sugared ferment of beta galactosidase, lactic dehydrogenase, hexokinase and pyruvate kinase etc. in fermentation process
Crucial enzyme activity in solution preocess accelerates the lactose fermentation of lactic acid bacteria, improves the lactic acid content in fermentation liquid.
Inventor, which passes through, finds a variety of saccharomycete to when screening: kluyveromyces marxianus bacterium is very suitable to celestial being
The utilization of ingredient is slapped, therefore, in the present invention, saccharomycete is preferably selected from kluyveromyces marxianus bacterium.
Two, bacillus subtilis
The effect of bacillus subtilis is to accelerate consumption of the cactus to oxygen at fermentation initial stage, accelerates anaerobic environment and is formed,
Promote anaerobic lactobacter growth, shortens fermentation period；And by generating a large amount of protease, lipase, cellulase and shallow lake
The enzymes such as powder enzyme improve the decomposition rate of albumen, lipid and polysaccharide component in cactus, enrich the amino acid and small in fermentation liquid
Molecular organic acids content, and nutrition is provided for lactic acid bacteria, promote Lactic Acid Secretion；By degradation of ammonia nitrogen, nitrite etc. it is harmful at
Part, improve the safety of fermentation liquid；By generating gramicidins isoreactivity substance, inhibit the growth of pernicious bacteria.
Three, lactobacillus plantarum
Lactobacillus plantarum is a kind of vegetal amphimicrobian lactic acid bacteria, is the key that strain of the present invention, in addition to
In generating outside lactic acid, also there are the oxious components such as degradation of ammonia nitrogen, nitrite, improve fermentation liquid safety, improve plant fermentation
The function of speed.
Four, lactobacillus acidophilus and Bifidobacterium
Lactobacillus acidophilus and Bifidobacterium are all anaerobism lactic acid bacterias, all have whole intestines effect, are the key that strains of the present invention.
Lactobacillus acidophilus needs acetate, vitamin and folic acid etc. as nutriment in growth, and Bifidobacterium removes in fermentation
It can generate outside acetic acid, moreover it is possible to synthesize the multivitamins such as vitamin B2 and folic acid, meet the needs of lactobacillus acidophilus just, promote
The growing multiplication of lactobacillus acidophilus.Since the two is difficult to individually cultivate, thus need to utilize two bacterium nutritionally institute in production
The complementary Replacement effect having, by being mixed to enhance the growth of two bacterium, accelerate to produce acid, shortening duration and improvement flavor.
Five, lactobacillus bulgaricus and streptococcus thermophilus
Lactobacillus bulgaricus and streptococcus thermophilus are all amphimicrobian lactic acid bacterias, and effect is to promote to coagulate in cactus
The decomposition of the effective components component such as glue, to enhance releasing for the nutrition such as triterpene, malic acid, succinic acid and alkaloid or edible composition
It puts, generate lactic acid and increases flavor.
It is of the invention that the specific implementation steps are as follows:
1, cactus removed the peel, be broken into fourth, be placed in round；The prior art that compares uses plastic barrel, plastics
The technical solution of cylinder or stainless steel storage tank, present invention selection are fermented in native terrine, can preferably retain the flavor of ferment.
2, sugar is added
In the present invention, brown sugar, molasses or maltose is added in selection, wherein if the sugar being added is brown sugar or molasses, sugar,
The quality proportioning of cactus and pure water is 1:1:1 or 1:3:1；If the sugar being added is maltose, sugar, cactus and pure
The quality proportioning of water purification is 1:1:1, and the quality of cactus here refers to the quality after peeling.
In the present invention, maltose fermentation is preferably selected, compared to more traditional technique fermented using brown sugar, inventor's warp
Comparative experiments discovery, after maltose fermentation, the nutritional ingredient of cactus enzyme product is richer, and mouthfeel is easier to be accepted
And absorption.
3, pure water is added.
4, be first added saccharomycete and bacillus subtilis, volume ratio between 1:4-1:1, preferably 1:2, probiotics it is total
The volume ratio of additive amount and cactus fermentation raw material is between 1%-2%；Room temperature sealed fermenting keeps one-way exhaust and slightly stirs
It mixes, until being no longer vented, this step is about in fermentation 7 days or so, to form anaerobic environment.
5, lactobacillus plantarum, lactobacillus acidophilus, Bifidobacterium, lactobacillus bulgaricus and streptococcus thermophilus are added,
Anaerobic fermentation, the volume parts being added respectively between: 0.8-1.2 parts, 2-4 parts, 2-4 parts, 1-3 parts and 1-3 parts, preferably respectively
It is 1 part, 3 parts, 3 parts, 2 parts and 2 parts, the total addition level of probiotics and the volume ratio of cactus fermentation raw material are between 2%-4%；Often
Warm sealed fermenting keeps one-way exhaust and gentle agitation, until being no longer vented, this step takes around fermentation 30-60 days and completes.
In the present invention, the viable count of the storage liquid for every kind of probiotics being added is between 1-2 × 104CFU/ml。
6, after fermentation in filtered under aseptic conditions.
7, filling under aseptic condition after initial survey is qualified, it examines, output finished product.
In the actual operation process, entire fermentation process is most short to be completed in five months the present invention, compare more existing skill
Art fermentation duration is up to 1 year fermentation process, and preparation method of the invention is substantially shorter fermentation period.
Table 1 is the composition analysis result of the obtained fermentation liquid of preparation method according to the present invention, as shown in table 1: this hair
The continuous analysis of the bright fermented liquid by the 1-8 month is sent out the results show that 5-8 month later fermentation liquid pH value stabilizes to 6
The slightly sour partially soft glutinous sweet tea of zymotic fluid mouthfeel, total viable count also tend to balance, and viable count is stablized 1 × 107/ ml compares more existing hair
Ferment technique, the present invention in each nutritional ingredient content it is also higher.
Table 1: the composition analysis result of the obtained fermentation liquid of preparation method according to the present invention
Beneficial effects of the present invention are as follows:
1, by the screening of a variety of probiotics, the safety of ferment product is increased, improves its active component content, is protected
The inherent quality for having demonstrate,proved finished product ferment is controllable.
2, entire fermentation period is most short to complete at five months, substantially reduce fermentation period, be fermented into reduce
This.
3, the fermentation liquid pH value finally prepared is stablized 6, on the one hand can inhibit the generation of miscellaneous bacteria, on the other hand enhance
The mouthfeel of product.
4, by the way that the better maltose of nutritional ingredient is added, make the ferment generated in actual use link, be more in line with height
The consumption habits for holding Crowded health protection nourishing beverage, more prepare public low side health beverages and provide ferment raw material.
5, whole anaerobic fermentation, it is thoroughly complete to guarantee fermentation, and fermentation system itself will not generate acetic acid bacteria and saccharomycete,
So as to more preferably guarantee flavor and the nutrition of original fermentation system, more conducively long-term preservation.
Although the present invention is illustrated using above-mentioned preferred embodiment, the protection model that however, it is not to limit the invention
It encloses, anyone skilled in the art are not departing within the spirit and scope of the present invention, and opposite above-described embodiment carries out various changes
It is dynamic still to belong to the range that the present invention is protected with modification, therefore protection scope of the present invention subjects to the definition of the claims.
Claims (8)
1. a kind of preparation method of cactus enzyme, which comprises the steps of:
Step S1: by cactus peeling, it is broken into fourth, is placed in round；
Step S2: sugar is added；
Step S3: pure water is added；
Step S4: mixing probiotics, anaerobic fermentation is added；
Step S5: after fermentation in filtered under aseptic conditions；
Step S6: it is filling under aseptic condition after initial survey is qualified, it examines, output finished product；
Wherein, in the step S2, the probiotics being added includes saccharomycete, bacillus subtilis, lactobacillus plantarum, acidophilus
Lactobacillus and Bifidobacterium.
2. the preparation method of cactus enzyme as described in claim 1, it is characterised in that: in the step S4, be added
Probiotics further includes lactobacillus bulgaricus and streptococcus thermophilus.
3. the preparation method of cactus enzyme as claimed in claim 2, which is characterized in that the step S4 includes:
Step S41: being added saccharomycete and bacillus subtilis, and room temperature sealed fermenting keeps one-way exhaust and gentle agitation, until
No longer it is vented；
Step S42: being added lactobacillus plantarum, lactobacillus acidophilus, Bifidobacterium, lactobacillus bulgaricus and streptococcus thermophilus,
Room temperature sealed fermenting keeps one-way exhaust and gentle agitation, until being no longer vented；
Wherein, in the step S41, the additive amount of probiotics and the volume ratio of cactus fermentation raw material are between 1%-2%；
In the step S42, the additive amount of probiotics and the volume ratio of cactus fermentation raw material are between 2%-4%；
Also, the viable count of the storage liquid for every kind of probiotics being added is between 1-2 × 104CFU/ml。
4. the preparation method of cactus enzyme as claimed in claim 3, it is characterised in that: in the step S41, saccharomycete and
The volume ratio of bacillus subtilis is between 1:4-1:1；In the step S42, lactobacillus plantarum, lactobacillus acidophilus, bifid bar
The volume parts that bacterium, lactobacillus bulgaricus and streptococcus thermophilus are added respectively between: 0.8-1.2 parts, 2-4 parts, 2-4 parts,
1-3 parts and 1-3 parts.
5. such as the preparation method of cactus enzyme of any of claims 1-4, it is characterised in that: the step S4
In, the saccharomycete being added is kluyveromyces marxianus bacterium.
6. such as the preparation method of cactus enzyme of any of claims 1-4, it is characterised in that: the step S1
In, used round is native terrine.
7. such as the preparation method of cactus enzyme of any of claims 1-4, it is characterised in that: the step S2
In, the sugar being added is brown sugar, molasses or maltose.
8. the preparation method of cactus enzyme as claimed in claim 7, it is characterised in that: in the step S2, if be added
Sugar is brown sugar or molasses, then the quality proportioning of sugar, cactus and pure water is 1:1:1 or 1:3:1；If the sugar being added is malt
Sugar, then the quality proportioning of sugar, cactus and pure water is 1:1:1.
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112617180A (en) * | 2020-12-21 | 2021-04-09 | 重庆八零四零生物工程研究院有限公司 | Comprehensive cactus enzyme |
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| WO2012097576A1 (en) * | 2011-01-21 | 2012-07-26 | 吉林紫鑫药物研究有限公司 | Method for preparing crop fermentation solution |
| CN103519171A (en) * | 2013-10-17 | 2014-01-22 | 天津市星河系科技有限公司 | Preparation method of cactus enzyme |
| CN105432912A (en) * | 2015-12-14 | 2016-03-30 | 中国科学院深圳先进技术研究院 | Plant ferment-filled candy and preparation method thereof |
| CN105942503A (en) * | 2016-05-09 | 2016-09-21 | 苏剑锋 | Preparation method of nycium and opuntia milpa alta ferment nutrition and ferment nutrition prepared by method |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| WO2012097576A1 (en) * | 2011-01-21 | 2012-07-26 | 吉林紫鑫药物研究有限公司 | Method for preparing crop fermentation solution |
| CN103519171A (en) * | 2013-10-17 | 2014-01-22 | 天津市星河系科技有限公司 | Preparation method of cactus enzyme |
| CN105432912A (en) * | 2015-12-14 | 2016-03-30 | 中国科学院深圳先进技术研究院 | Plant ferment-filled candy and preparation method thereof |
| CN105942503A (en) * | 2016-05-09 | 2016-09-21 | 苏剑锋 | Preparation method of nycium and opuntia milpa alta ferment nutrition and ferment nutrition prepared by method |
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| CN112617180A (en) * | 2020-12-21 | 2021-04-09 | 重庆八零四零生物工程研究院有限公司 | Comprehensive cactus enzyme |
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