CN109953023A

CN109953023A - A kind of attractant and its luring the application in bollworm

Info

Publication number: CN109953023A
Application number: CN201711433088.6A
Authority: CN
Inventors: 王桂荣; 郭孟博; 刘杨
Original assignee: Institute of Plant Protection of Chinese Academy of Agricultural Sciences
Current assignee: Institute of Plant Protection of Chinese Academy of Agricultural Sciences
Priority date: 2017-12-26
Filing date: 2017-12-26
Publication date: 2019-07-02

Abstract

This application involves a kind of attractant and its luring the application in bollworm.Wherein, attractant includes solvent, cinnamic acid, salicylide, 2- methyl o-anisate, linalool and limonene.

Description

A kind of attractant and its luring the application in bollworm

Technical field

This application involves a kind of attractants, in particular to the attractant is luring the application in bollworm.

Background technique

Bollworm (Helicoverpa armigera), belongs to Lepidoptera, noctuidae pests, in the multiple provinces of China and generation Other areas are widely distributed in boundary.Its larva has height omnivorousness, and feeding includes that corn and soybean, cotton, tomato etc. mainly pass through 120 various plants including Ji crop, cause serious economic loss.Its adult has high mobility, and female adult has very strong Egg production capacity, highest can produce more than 1000 ovum, and male worm be reported can multiple mating, therefore the prevention and treatment of bollworm is very big Problem.The large area use of pesticide brings various problems, such as ecological pollution, pest resistance increase etc..Integrated pest management (IPM) Always by multiple countries support energetically in the world control strategy.(such as turn Bt cotton in the popularizing planting of China, genetically modified crops Flower, turn Bt corn) efficiently controlled bollworm large area harm.However, bollworm is more to Bt generation resistance in recent years It has been reported that, it is anticipated that genetically modified crops are by the increased increasingly stern challenge of the target pest resistance faced.

The important function that plant volatile plays in the important biomolecules activity such as insect's food-taking, mating, oviposition by Sufficiently prove.For bollworm, many Behaviors surveys show a variety of volatile matter groups of host plant such as cotton, soybean, tobacco Moth female to its, male is divided to have attracting action.

Bollworm female, male imago scope of activities are wider, and female moth is with the presence of very strong egg production capacity and male moth multiple mating Phenomenon, therefore trap female, male moth simultaneously and will play a significant role to cotton bollworm control.All having after emergence finds nectar source feeding Characteristic, and female moth using plant volatile find oviposition host plant therefore use the plant source chemical combination with sucking action Object or composition will provide means a kind of effective and that ecology is friendly as attractant for the comprehensive treatment of bollworm, Thus it is of great significance.

Summary of the invention

One of the application provides a kind of attractant comprising solvent, cinnamic acid, salicylide, 2- o-methoxybenzoic acid Methyl esters, linalool and limonene.

In a specific embodiment, by volume, content of the cinnamic acid in attractant is 0.1 ‰ to 1 ‰； Content of the salicylide in attractant is 0.1 ‰ to 0.5 ‰；The 2- methyl o-anisate is in attractant Content is 0.1 ‰ to 0.5 ‰；Content of the linalool in attractant is 0.05 ‰ to 0.2 ‰；The limonene is being lured Content in agent is 0.05 ‰ to 0.2 ‰.

In a specific embodiment, by volume, content of the cinnamic acid in attractant be 0.3 ‰ to 0.8‰；Content of the salicylide in attractant is 0.1 ‰ to 0.5 ‰；The 2- methyl o-anisate is being lured Content 0.1 ‰ to 0.5 ‰ in agent；Content of the linalool in attractant is 0.05 ‰ to 0.2 ‰；The limonene exists Content in attractant is 0.05 ‰ to 0.2 ‰.

In a specific embodiment, by volume, content of the cinnamic acid in attractant be 0.3 ‰ to 0.8‰；Content of the salicylide in attractant is 0.1 ‰ to 0.3 ‰；Content of the linalool in attractant be 0.05 ‰ to 0.1 ‰；Content 0.1 ‰ to 0.3 ‰ of the 2- methyl o-anisate in attractant；The limonene Content in attractant is 0.05 ‰ to 0.1 ‰.

In a specific embodiment, by volume, content of the cinnamic acid in attractant is 0.5 ‰；It is described Content of the salicylide in attractant is 0.2 ‰；Content 0.2 ‰ of the 2- methyl o-anisate in attractant； Content of the linalool in attractant is 0.1 ‰；Content of the limonene in attractant is 0.1 ‰.

Those skilled in the art can select such as n-hexane, paraffin oil or mineral oil as attractant according to conventional Solvent.Therefore, in a specific embodiment, the solvent is selected from least one of n-hexane, paraffin oil, mineral oil.

The two of the application provide attractant described in one of the application any one for luring answering in bollworm With.

The application's the utility model has the advantages that

The application has found that the HarmOR59 gene of bollworm is odorant receptor genes for the first time, and the albumen generated is to cinnamic acid With very strong sensibility.And it is based on this, it forms one kind and lures the efficient attractant of bollworm.

Specific embodiment

The above content of the application is described in further detail below by way of the form of preferred embodiment, but not structure At limitation of the present invention.

Unless otherwise instructed, the reagent in embodiments herein can be bought by commercial sources.The chemistry examination of selection Agent is that analysis is pure.

Embodiment 1

1. the clone of bollworm odorant receptor genes HarmOR59 and cRNA synthesis

1.1 take 1-3 days after emergence bollworm feelers, extract RNA and are simultaneously inverted to cDNA；

1.2 according to the mRNA of HarmOR59, (SEQ ID No.1 corresponds to the SEQ ID of the amino acid sequence of coding No.2) sequence, design primer OR59-F (SEQ ID No.3) and primer OR59-R (SEQ ID No.4), to be obtained in step 1.1 The cDNA obtained is the coded sequence (SEQ ID No.1) that template clones HarmOR59, and connection is cloned on load pMD-19, is transferred to large intestine 37 DEG C of overnight incubations of bacillus competent cell choose monoclonal and determining gene order are sequenced, obtain pMD-HarmOR59；

1.3 synthesis connect expression vector primer OR59-PT7Ts-F (SEQ ID No.5), OR59-PT7Ts-R (SEQ ID No.6), the composition of OR59-PT7Ts-F primer are as follows: 20 nucleotide sequences identical with expression vector upstream (acgctcaacttggcagatct)+Kozak sequence (gccacc)+overall length primer OR59-F (SEQ ID No.3)；OR59- The composition of PT7Ts-R primer are as follows: 17 nucleotide sequences (gatcctagtcagtcactagt) identical with expression vector downstream+ Overall length primer OR59-R (SEQ ID No.4), using pMD-HarmOR59 plasmid as template progress PCR clone gene overall length, after PCR Product is purified；By expression vector PT7Ts plasmid (Addgene plasmid#17091) with restriction enzyme BglII, SpeI carries out double digestion, and digestion products connect that (promise is only praised with the PCR product of above-mentioned purifying by homologous recombination mode after purification C112-01), it is transferred to 37 DEG C of overnight incubations of competent escherichia coli cell Trans-T1 (Quan Shijin) later, picking monoclonal is surveyed Sequence determines gene order, obtains the eukaryotic expression plasmid HarmOR59-PT7Ts of connection HarmOR59 complete encoding sequence.

2. bollworm odorant receptor cRNA synthesis and heterogenous expression

The Restriction enzyme Sma I digestion of HarmOR59-PT7Ts plasmid, digestion products are passed through phenol/chloroform by 2.1 It purified, be concentrated, later using digestion products after purification as template, synthesized with in-vitro transcription kit (T7Kit, Ambion) The cRNA (SEQ ID No.7) of HarmOR59 comprising successively from 5 ' to 3 ' SEQ ID No.8, SEQ ID No.1 and SEQ ID No.9.CRNA is purified and determines concentration to 2000ng/ μ l, it is spare that -80 DEG C of refrigerators are put in after packing.

2.2 co-express odorant receptor HarmORco (SEQ ID No.10, correspondence coding with above-mentioned same method clone Amino acid sequence SEQ ID No.11) DNA sequence dna (SEQ ID No.10).And synthesize the cRNA (SEQ of HarmORco ID No.12) comprising successively from 5 ' to 3 ' SEQ ID No.8, SEQ ID No.10 and SEQ ID No.9.CRNA is pure Change and determine concentration to 2000ng/ μ l, it is spare that -80 DEG C of refrigerators are put in after packing.The primer used when being connected on cloning vector are as follows: ORco-F (SEQ ID No.13), ORco-R (SEQ ID No.14), the primer used when being connected on expression vector are (primer Constitute identical with HarmOR59): ORco-PT7Ts-F (SEQ ID No.15), ORco-PT7Ts-R (SEQ ID No.16).

2.3 uniformly mix each 1 μ l of the cRNA of HarmOR59 and HarmORco (in insect, special odorant receptor egg White needs and atypical coexpression odorant receptor ORco coexpression, form the effect of dimer competence exertion), use microinjection instrument The cRNA mixed is injected into the ovum of Africa xenopus, the injection volume of every ovum is 27.6nl, and the ovum after injection is trained at 16 DEG C It is cultivated in nutrient solution.

3. voltage-clamp recording

3.1 by the above-mentioned 2.3 egg mother cell culture injected 2-5 days；

3.2 are respectively configured to 13 kinds of selected plant volatiles with dimethyl sulfoxide (DMSO) in the mother liquor of 1mol/L, then With 1 × Ringer (9.6mM NaCl, 0.2mM KCl, 0.5mM MgCl₂-6H₂O,0.5mM HEPES,0.6mM CaCl₂,pH 7.6) it is configured to the perfusate of final concentration of 10-4mol/L；Wherein, 13 kinds of plant volatiles are successively are as follows: cinnamic acid, laurene, Myrte, piperitone, methyl benzoate, 4- ethyl acetophenone, 4- methoxy benzyl alcohol, eugenol, trans- 3- hexenol, N-octyl alcohol, 1-OCOL, cis- 3- hexenyl acetate, geranyl acetate.

3.3 express the egg mother cell of HarmOR59 to above-mentioned 13 kinds of plant volatiles using Two-electrode voltage-clamp system measurement Current value, 4-8 cell of every kind of compound determination.The result shows that HarmOR59 can be by cinnamic acid strong activation, 10^-4M is dense Wherein maximum reacting value reaches 1000nA to the lower reaction average value 700nA of degree, remaining has the compound of reaction 10^-4It is as follows under M concentration: Methyl phenylacetate (283nA), 4- ethyl acetophenone (310nA), trans- 3- hexenol (364nA), n-octyl alcohol (367nA).

The EC of the egg mother cell of HarmOR59 is expressed in the activation such as 3.4 cinnamic acids₅₀

Cinnamic acid under various concentration, methyl phenylacetate, 4- ethyl acetophenone, trans- 3- hexenol, n-octyl alcohol is measured respectively to swash The response value of receptor living, and dose-response curve is drawn, calculate EC₅₀Value.The result shows that HarmOR59 has highest clever cinnamic acid Sensitivity, EC₅₀Value is 1.716e-006mol/L.To the EC of other compounds₅₀Value is respectively as follows: methyl phenylacetate (2.826e- 004mol/L), 4- ethyl acetophenone (7.246e-005mol/L), trans- 3- hexenol (4.945e-005mol/L), n-octyl alcohol (4.116e-004mol/L)。

Embodiment 2

The preparation of attractant mother liquor:

It is adjacent to draw 50 μ L cinnamic acids (Cinnamaldehyde), 20 μ L salicylides (Salicylaldehyde), 10 μ L 2- Methoxyl methyl benzoate (Methyl 2-methoxybenzoate), 10 μ L linalools (Linalool), 10 μ L limonenes ((R)-(+)-Limonene) is uniformly mixed.Attractant mother liquor after being made can be put into refrigerator preservation, and storage temperature is -22 ℃。

The preparation of attractant:

Attractant mother liquor n-hexane is pressed 1000 times of gradient dilution, obtain the cinnamic acid of 0.5v ‰, ‰ salicylide of 0.2v, The attractant of the limonene of the 2- methyl o-anisate of 0.2v ‰, the linalool of 0.1v ‰ and 0.1v ‰.

Using solvent hexane as negative control.

Comparative example 1

Compare the preparation of attractant mother liquor:

Draw 20 μ L salicylides (Salicylaldehyde), 10 μ L 2- methyl o-anisate (Methyl 2- Methoxybenzoate), 10 μ L linalools (Linalool), 10 μ L limonenes ((R)-(+)-Limonene), use n-hexane (Hexane) 100 μ L are settled to.It is uniformly mixed.Comparison attractant mother liquor after being made can be put into refrigerator preservation, storage temperature It is -22 DEG C.

Compare the preparation of attractant:

1000 times of gradient dilution are pressed with n-hexane in use, attractant mother liquor will be compared, is uniformly mixed.Obtain ‰ water of 0.2v Poplar aldehyde, the 2- methyl o-anisate of 0.2v ‰, the linalool of 0.1v ‰ and 0.1v ‰ limonene attractant.

Using solvent hexane as negative control.

Embodiment 3

Lure experiment

Indoor Y tube trapping experiment is carried out to attractant.

Place: test carries out in Plant Protection institute, Chinese Academy of Agricultral Sciences laboratory.

Time: test period is evening 18:00 to morning 2:00.

Indoor conditions: 26~28 DEG C of temperature, humidity 30%~50%；Light impacts action selection in order to prevent, Use feux rouges as light source.

Instrument and equipment and setting: Y tube is glass material, and 60 ° of two side arms A, B angle, long 20cm, straight-arm are long respectively 25cm, three arm diameters are 4cm, and two side arm openings are clogged with the rubber stopper that centre is inserted with sebific duct respectively；Air-flow is big After gas sampling instrument is collected, tower, Zhi Houfen are moistened by the glass drying tower equipped with active carbon and the glass equipped with pure water respectively Stream enters two-arm, air velocity 1L/min.

Behaviouristics measurement:

(1) 50 μ L of prepared attractant (attractant of embodiment 1 or the comparison attractant of comparative example 1) are drawn to be added drop-wise to On the rectangular filter paper of side length 1cm, wait the scraps of paper are put into A arm after the solvent is volatilized within 10 seconds, B arm place be added dropwise 50 μ L just oneself The scraps of paper of alkane are as negative control；

(2) bollworm female, the male moth single head sprouted wings 1~2 day are put into straight-arm inlet, open atmosphere sampling instrument make air-flow into Enter, starts simultaneously at timing, cotton bollworm moth is respectively in the residence time of two-arm in statistics 10min；

(3) Y tube and filter paper are replaced after every cotton bollworm moth test, and replaces the left-right position of A, B two-arm, at every group In Behavior Test, the borer population that female, male moth uses is respectively 50.

Interpretation of result:

It counts female, male moth respectively to be inclined to the selection of different attractants, the individual of selection is not done in removal, is calculated and is selected each arm Polypide number Zhan always select the percentage of polypide number, calculation formula are as follows: lure rate=selection attractant number of individuals/(selection is lured Agent number of individuals+selection n-hexane number of individuals).The result shows that: the attractant of embodiment 2 is 58.8% to the rate of luring of female moth, right The rate of luring of male moth is 66.6%；And comparative example 1 is 37% to the rate of luring of female moth, the rate of luring to male moth is 40.7%.It is right Ratio 1 only lacks cinnamic acid compared with the formula of embodiment 2, shows that cinnamic acid plays a significant role in luring agent composition. The attractant effect to bollworm female moth that the attractant of embodiment 2 obtains is higher than the attractant of comparative example 1 to bollworm female moth Attractant effect 21.8%；The attractant effect to bollworm hero moth that the attractant of embodiment 2 obtains is higher than the attractant of comparative example 1 To the attractant effect 25.9% of bollworm hero moth.And then it concludes that the attractant containing cinnamic acid has and preferably lures work With.

Sequence table

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ggctccgagg aagcgaagac attcgtgcaa atcgtctgcc aacagtgtca gaaggctatg 1320

agcatctccg gagccaagtt cttcacggtc tcacttgatt tgtttgcttc ggtccttgga 1380

gccgtggtaa cctacttcat ggtattgata cagctcaagt ga 1422

<210> 11

<211> 473

<212> PRT

<213>bollworm (Helicoverpa armigera)

<400> 11

Met Met Thr Lys Val Lys Ala Gln Gly Leu Val Ser Asp Leu Met Pro

1 5 10 15

Asn Ile Lys Leu Met Gln Met Ala Gly His Phe Leu Phe Asn Tyr His

20 25 30

Ser Glu Asn Ala Gly Met Ser Asn Leu Leu Arg Lys Ile Tyr Ala Ser

35 40 45

Thr His Ala Ile Leu Ile Phe Ile His Tyr Ala Cys Met Gly Ile Asn

50 55 60

Met Ala Lys Tyr Ser Asp Glu Val Asn Glu Leu Thr Ala Asn Thr Ile

65 70 75 80

Thr Val Leu Phe Phe Ala His Thr Ile Ile Lys Leu Ala Phe Phe Ala

85 90 95

Leu Asn Ser Lys Ser Phe Tyr Arg Thr Leu Ala Val Trp Asn Gln Ser

100 105 110

Asn Ser His Pro Leu Phe Thr Glu Ser Asp Ala Arg Tyr His Gln Ile

115 120 125

Ala Leu Thr Lys Met Arg Arg Leu Leu Tyr Phe Ile Cys Gly Met Thr

130 135 140

Val Leu Ser Val Ile Ser Trp Val Thr Leu Thr Phe Phe Gly Glu Ser

145 150 155 160

Val Arg Met Val Thr Asn Lys Glu Thr Asn Glu Thr Leu Thr Glu Val

165 170 175

Val Pro Arg Leu Pro Leu Lys Ala Trp Tyr Pro Phe Asn Ala Met Ser

180 185 190

Gly Thr Met Tyr Ile Val Ala Phe Ala Phe Gln Val Tyr Trp Leu Leu

195 200 205

Phe Ser Met Ala Ile Ala Asn Leu Met Asp Val Met Phe Cys Ser Trp

210 215 220

Leu Ile Phe Ala Cys Glu Gln Leu Gln His Leu Lys Ala Ile Met Lys

225 230 235 240

Pro Leu Met Glu Leu Ser Ala Ser Leu Asp Thr Tyr Arg Pro Asn Thr

245 250 255

Ala Glu Leu Phe Arg Ala Ser Ser Thr Glu Lys Ser Glu Lys Ile Pro

260 265 270

Asp Thr Val Asp Met Asp Ile Arg Gly Ile Tyr Ser Thr Gln Gln Asp

275 280 285

Phe Gly Met Thr Leu Arg Gly Ala Gly Gly Arg Leu Gln Asn Phe Gly

290 295 300

Gln Gln Asn Pro Asn Pro Asn Gly Leu Thr Pro Lys Gln Glu Met Leu

305 310 315 320

Ala Arg Ser Ala Ile Lys Tyr Trp Val Glu Arg His Lys His Val Val

325 330 335

Arg Leu Val Ala Ser Ile Gly Asp Thr Tyr Gly Thr Ala Leu Leu Phe

340 345 350

His Met Leu Val Ser Thr Ile Thr Leu Thr Leu Leu Ala Tyr Gln Ala

355 360 365

Thr Lys Ile Asn Gly Ile Asn Val Tyr Ala Phe Ser Thr Ile Gly Tyr

370 375 380

Leu Ser Tyr Thr Leu Gly Gln Val Phe His Phe Cys Ile Phe Gly Asn

385 390 395 400

Arg Leu Ile Glu Glu Ser Ser Ser Val Met Glu Ala Ala Tyr Ser Cys

405 410 415

Gln Trp Tyr Asp Gly Ser Glu Glu Ala Lys Thr Phe Val Gln Ile Val

420 425 430

Cys Gln Gln Cys Gln Lys Ala Met Ser Ile Ser Gly Ala Lys Phe Phe

435 440 445

Thr Val Ser Leu Asp Leu Phe Ala Ser Val Leu Gly Ala Val Val Thr

450 455 460

Tyr Phe Met Val Leu Ile Gln Leu Lys

465 470

<210> 12

<211> 1703

<212> DNA/RNA

<213>artificial sequence (non)

<400> 12

aagcttgctt gttctttttg cagaagctca gaaacgctca acttggcaga tctgccacca 60

tgatgaccaa ggtgaaggcc cagggcctcg tgtcagactt gatgcccaac atcaagctga 120

tgcagatggc cggccatttc ctcttcaatt accactcaga aaatgctggc atgtcaaacc 180

ttctccgtaa gatctacgcg agtactcatg ctatcctcat tttcatccac tacgcgtgta 240

tgggaatcaa catggcgaaa tactccgatg aggtcaacga gctgacagcc aataccataa 300

ctgtcttatt cttcgcccat actatcatca agcttgcgtt ctttgcttta aactctaaga 360

gcttctacag gaccctggca gtatggaacc agtcgaacag tcacccactg ttcacggagt 420

cagacgctcg ctatcaccag atcgcgctca ccaagatgag gaggctgctg tacttcatct 480

gcgggatgac tgtcctctct gttatcagct gggtaaccct aacattcttc ggtgagtcag 540

tacgcatggt gacgaacaag gaaaccaacg agaccctgac ggaggtggtg ccccgcctgc 600

ctctgaaggc ctggtaccct tttaatgcta tgagcggcac tatgtatatc gtggcgttcg 660

cttttcaggt atactggctc ctattctcaa tggccatagc gaacctgatg gacgtgatgt 720

tctgttcctg gctgatcttc gcgtgtgaac agctgcagca tttgaaggct atcatgaaac 780

ccctcatgga gttgagcgcc tctttggaca cgtaccggcc taatactgct gagctgttta 840

gagcttcttc tactgagaaa tccgaaaaga tccccgacac ggtagacatg gacattcgcg 900

gcatatactc cacacagcaa gacttcggca tgacactccg aggtgctggt gggagactcc 960

agaacttcgg ccagcagaac cccaacccta acggcttgac tcccaagcag gagatgctgg 1020

ccaggtctgc tatcaagtac tgggtggaga ggcataagca tgtcgtcaga ctagtggcat 1080

cgattggaga cacgtatggt accgccctgc tgttccacat gttggtgtct acgatcacac 1140

tcaccctgct ggcctaccaa gctactaaga tcaacggaat caacgtgtat gctttcagta 1200

cgatcggata cttgagttac actctgggtc aagtgttcca cttctgcatc tttggaaata 1260

ggctcattga agagagctca tcagtaatgg aagcagctta ctcctgccag tggtatgacg 1320

gctccgagga agcgaagaca ttcgtgcaaa tcgtctgcca acagtgtcag aaggctatga 1380

gcatctccgg agccaagttc ttcacggtct cacttgattt gtttgcttcg gtccttggag 1440

ccgtggtaac ctacttcatg gtattgatac agctcaagtg aactagtgac tgactaggat 1500

ctggttacca ctaaaccagc ctcaagaaca cccgaatgga gtctctaagc tacataatac 1560

caacttacac ttacaaaatg ttgtccccca aaatgtagcc attcgtatct gctcctaata 1620

aaaagaaagt ttcttcacat tctaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaccccccc 1680

cccccccccc cccccccccc ccc 1703

<210> 13

<211> 21

<212> DNA

<213>artificial sequence (non)

<400> 13

atgatgacca aggtgaaggc c 21

<210> 14

<211> 22

<212> DNA

<213>artificial sequence (non)

<400> 14

ttacttgagc tgtatcaata cc 22

<210> 15

<211> 47

<212> DNA

<213>artificial sequence (non)

<400> 15

acgctcaact tggcagatct gccaccatga tgaccaaggt gaaggcc 47

<210> 16

<211> 42

<212> DNA

<213>artificial sequence (non)

<400> 16

gatcctagtc agtcactagt ttacttgagc tgtatcaata cc 42

Claims

1. a kind of attractant comprising solvent, cinnamic acid, salicylide, 2- methyl o-anisate, linalool and lemon Alkene.

2. attractant according to claim 1, which is characterized in that by volume, the cinnamic acid containing in attractant Amount is 0.1 ‰ to 1 ‰；Content of the salicylide in attractant is 0.1 ‰ to 0.5 ‰；The 2- o-methoxybenzoic acid Content of the methyl esters in attractant is 0.1 ‰ to 0.5 ‰；Content of the linalool in attractant is 0.05 ‰ to 0.2 ‰； Content of the limonene in attractant is 0.05 ‰ to 0.2 ‰.

3. attractant according to claim 2, which is characterized in that by volume, the cinnamic acid containing in attractant Amount is 0.3 ‰ to 0.8 ‰；Content of the salicylide in attractant is 0.1 ‰ to 0.5 ‰；The 2- O-methoxy benzene first Content 0.1 ‰ to 0.5 ‰ of the sour methyl esters in attractant；Content of the linalool in attractant is 0.05 ‰ to 0.2 ‰； Content of the limonene in attractant is 0.05 ‰ to 0.2 ‰.

4. attractant according to claim 3, which is characterized in that by volume, the cinnamic acid containing in attractant Amount is 0.3 ‰ to 0.8 ‰；Content of the salicylide in attractant is 0.1 ‰ to 0.3 ‰；The linalool is in attractant In content be 0.1 ‰ to 0.3 ‰；Content 0.05 ‰ to 0.1 ‰ of the 2- methyl o-anisate in attractant； Content of the limonene in attractant is 0.05 ‰ to 0.1 ‰.

5. attractant according to claim 4, which is characterized in that by volume, the cinnamic acid containing in attractant Amount is 0.5 ‰；Content of the salicylide in attractant is 0.2 ‰；The 2- methyl o-anisate is in attractant In content 0.2 ‰；Content of the linalool in attractant is 0.1 ‰；Content of the limonene in attractant be 0.1‰。

6. attractant as claimed in any of claims 1 to 5, which is characterized in that the solvent is selected from n-hexane, stone At least one of wax oil, mineral oil.

7. attractant as claimed in any of claims 1 to 6 is for luring the application in bollworm.