CN109953023A - A kind of attractant and its luring the application in bollworm - Google Patents
A kind of attractant and its luring the application in bollworm Download PDFInfo
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- CN109953023A CN109953023A CN201711433088.6A CN201711433088A CN109953023A CN 109953023 A CN109953023 A CN 109953023A CN 201711433088 A CN201711433088 A CN 201711433088A CN 109953023 A CN109953023 A CN 109953023A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N27/00—Biocides, pest repellants or attractants, or plant growth regulators containing hydrocarbons
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N35/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical
- A01N35/02—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical containing aliphatically bound aldehyde or keto groups, or thio analogues thereof; Derivatives thereof, e.g. acetals
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N35/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical
- A01N35/04—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical containing aldehyde or keto groups, or thio analogues thereof, directly attached to an aromatic ring system, e.g. acetophenone; Derivatives thereof, e.g. acetals
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/36—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids
- A01N37/38—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids having at least one oxygen or sulfur atom attached to an aromatic ring system
- A01N37/40—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids having at least one oxygen or sulfur atom attached to an aromatic ring system having at least one carboxylic group or a thio analogue, or a derivative thereof, and one oxygen or sulfur atom attached to the same aromatic ring system
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N49/00—Biocides, pest repellants or attractants, or plant growth regulators, containing compounds containing the group, wherein m+n>=1, both X together may also mean —Y— or a direct carbon-to-carbon bond, and the carbon atoms marked with an asterisk are not part of any ring system other than that which may be formed by the atoms X, the carbon atoms in square brackets being part of any acyclic or cyclic structure, or the group, wherein A means a carbon atom or Y, n>=0, and not more than one of these carbon atoms being a member of the same ring system, e.g. juvenile insect hormones or mimics thereof
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- Plant Pathology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Agronomy & Crop Science (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Insects & Arthropods (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
This application involves a kind of attractant and its luring the application in bollworm.Wherein, attractant includes solvent, cinnamic acid, salicylide, 2- methyl o-anisate, linalool and limonene.
Description
Technical field
This application involves a kind of attractants, in particular to the attractant is luring the application in bollworm.
Background technique
Bollworm (Helicoverpa armigera), belongs to Lepidoptera, noctuidae pests, in the multiple provinces of China and generation
Other areas are widely distributed in boundary.Its larva has height omnivorousness, and feeding includes that corn and soybean, cotton, tomato etc. mainly pass through
120 various plants including Ji crop, cause serious economic loss.Its adult has high mobility, and female adult has very strong
Egg production capacity, highest can produce more than 1000 ovum, and male worm be reported can multiple mating, therefore the prevention and treatment of bollworm is very big
Problem.The large area use of pesticide brings various problems, such as ecological pollution, pest resistance increase etc..Integrated pest management (IPM)
Always by multiple countries support energetically in the world control strategy.(such as turn Bt cotton in the popularizing planting of China, genetically modified crops
Flower, turn Bt corn) efficiently controlled bollworm large area harm.However, bollworm is more to Bt generation resistance in recent years
It has been reported that, it is anticipated that genetically modified crops are by the increased increasingly stern challenge of the target pest resistance faced.
The important function that plant volatile plays in the important biomolecules activity such as insect's food-taking, mating, oviposition by
Sufficiently prove.For bollworm, many Behaviors surveys show a variety of volatile matter groups of host plant such as cotton, soybean, tobacco
Moth female to its, male is divided to have attracting action.
Bollworm female, male imago scope of activities are wider, and female moth is with the presence of very strong egg production capacity and male moth multiple mating
Phenomenon, therefore trap female, male moth simultaneously and will play a significant role to cotton bollworm control.All having after emergence finds nectar source feeding
Characteristic, and female moth using plant volatile find oviposition host plant therefore use the plant source chemical combination with sucking action
Object or composition will provide means a kind of effective and that ecology is friendly as attractant for the comprehensive treatment of bollworm,
Thus it is of great significance.
Summary of the invention
One of the application provides a kind of attractant comprising solvent, cinnamic acid, salicylide, 2- o-methoxybenzoic acid
Methyl esters, linalool and limonene.
In a specific embodiment, by volume, content of the cinnamic acid in attractant is 0.1 ‰ to 1 ‰;
Content of the salicylide in attractant is 0.1 ‰ to 0.5 ‰;The 2- methyl o-anisate is in attractant
Content is 0.1 ‰ to 0.5 ‰;Content of the linalool in attractant is 0.05 ‰ to 0.2 ‰;The limonene is being lured
Content in agent is 0.05 ‰ to 0.2 ‰.
In a specific embodiment, by volume, content of the cinnamic acid in attractant be 0.3 ‰ to
0.8‰;Content of the salicylide in attractant is 0.1 ‰ to 0.5 ‰;The 2- methyl o-anisate is being lured
Content 0.1 ‰ to 0.5 ‰ in agent;Content of the linalool in attractant is 0.05 ‰ to 0.2 ‰;The limonene exists
Content in attractant is 0.05 ‰ to 0.2 ‰.
In a specific embodiment, by volume, content of the cinnamic acid in attractant be 0.3 ‰ to
0.8‰;Content of the salicylide in attractant is 0.1 ‰ to 0.3 ‰;Content of the linalool in attractant be
0.05 ‰ to 0.1 ‰;Content 0.1 ‰ to 0.3 ‰ of the 2- methyl o-anisate in attractant;The limonene
Content in attractant is 0.05 ‰ to 0.1 ‰.
In a specific embodiment, by volume, content of the cinnamic acid in attractant is 0.5 ‰;It is described
Content of the salicylide in attractant is 0.2 ‰;Content 0.2 ‰ of the 2- methyl o-anisate in attractant;
Content of the linalool in attractant is 0.1 ‰;Content of the limonene in attractant is 0.1 ‰.
Those skilled in the art can select such as n-hexane, paraffin oil or mineral oil as attractant according to conventional
Solvent.Therefore, in a specific embodiment, the solvent is selected from least one of n-hexane, paraffin oil, mineral oil.
The two of the application provide attractant described in one of the application any one for luring answering in bollworm
With.
The application's the utility model has the advantages that
The application has found that the HarmOR59 gene of bollworm is odorant receptor genes for the first time, and the albumen generated is to cinnamic acid
With very strong sensibility.And it is based on this, it forms one kind and lures the efficient attractant of bollworm.
Specific embodiment
The above content of the application is described in further detail below by way of the form of preferred embodiment, but not structure
At limitation of the present invention.
Unless otherwise instructed, the reagent in embodiments herein can be bought by commercial sources.The chemistry examination of selection
Agent is that analysis is pure.
Embodiment 1
1. the clone of bollworm odorant receptor genes HarmOR59 and cRNA synthesis
1.1 take 1-3 days after emergence bollworm feelers, extract RNA and are simultaneously inverted to cDNA;
1.2 according to the mRNA of HarmOR59, (SEQ ID No.1 corresponds to the SEQ ID of the amino acid sequence of coding
No.2) sequence, design primer OR59-F (SEQ ID No.3) and primer OR59-R (SEQ ID No.4), to be obtained in step 1.1
The cDNA obtained is the coded sequence (SEQ ID No.1) that template clones HarmOR59, and connection is cloned on load pMD-19, is transferred to large intestine
37 DEG C of overnight incubations of bacillus competent cell choose monoclonal and determining gene order are sequenced, obtain pMD-HarmOR59;
1.3 synthesis connect expression vector primer OR59-PT7Ts-F (SEQ ID No.5), OR59-PT7Ts-R (SEQ ID
No.6), the composition of OR59-PT7Ts-F primer are as follows: 20 nucleotide sequences identical with expression vector upstream
(acgctcaacttggcagatct)+Kozak sequence (gccacc)+overall length primer OR59-F (SEQ ID No.3);OR59-
The composition of PT7Ts-R primer are as follows: 17 nucleotide sequences (gatcctagtcagtcactagt) identical with expression vector downstream+
Overall length primer OR59-R (SEQ ID No.4), using pMD-HarmOR59 plasmid as template progress PCR clone gene overall length, after PCR
Product is purified;By expression vector PT7Ts plasmid (Addgene plasmid#17091) with restriction enzyme BglII,
SpeI carries out double digestion, and digestion products connect that (promise is only praised with the PCR product of above-mentioned purifying by homologous recombination mode after purification
C112-01), it is transferred to 37 DEG C of overnight incubations of competent escherichia coli cell Trans-T1 (Quan Shijin) later, picking monoclonal is surveyed
Sequence determines gene order, obtains the eukaryotic expression plasmid HarmOR59-PT7Ts of connection HarmOR59 complete encoding sequence.
2. bollworm odorant receptor cRNA synthesis and heterogenous expression
The Restriction enzyme Sma I digestion of HarmOR59-PT7Ts plasmid, digestion products are passed through phenol/chloroform by 2.1
It purified, be concentrated, later using digestion products after purification as template, synthesized with in-vitro transcription kit (T7Kit, Ambion)
The cRNA (SEQ ID No.7) of HarmOR59 comprising successively from 5 ' to 3 ' SEQ ID No.8, SEQ ID No.1 and SEQ
ID No.9.CRNA is purified and determines concentration to 2000ng/ μ l, it is spare that -80 DEG C of refrigerators are put in after packing.
2.2 co-express odorant receptor HarmORco (SEQ ID No.10, correspondence coding with above-mentioned same method clone
Amino acid sequence SEQ ID No.11) DNA sequence dna (SEQ ID No.10).And synthesize the cRNA (SEQ of HarmORco
ID No.12) comprising successively from 5 ' to 3 ' SEQ ID No.8, SEQ ID No.10 and SEQ ID No.9.CRNA is pure
Change and determine concentration to 2000ng/ μ l, it is spare that -80 DEG C of refrigerators are put in after packing.The primer used when being connected on cloning vector are as follows:
ORco-F (SEQ ID No.13), ORco-R (SEQ ID No.14), the primer used when being connected on expression vector are (primer
Constitute identical with HarmOR59): ORco-PT7Ts-F (SEQ ID No.15), ORco-PT7Ts-R (SEQ ID No.16).
2.3 uniformly mix each 1 μ l of the cRNA of HarmOR59 and HarmORco (in insect, special odorant receptor egg
White needs and atypical coexpression odorant receptor ORco coexpression, form the effect of dimer competence exertion), use microinjection instrument
The cRNA mixed is injected into the ovum of Africa xenopus, the injection volume of every ovum is 27.6nl, and the ovum after injection is trained at 16 DEG C
It is cultivated in nutrient solution.
3. voltage-clamp recording
3.1 by the above-mentioned 2.3 egg mother cell culture injected 2-5 days;
3.2 are respectively configured to 13 kinds of selected plant volatiles with dimethyl sulfoxide (DMSO) in the mother liquor of 1mol/L, then
With 1 × Ringer (9.6mM NaCl, 0.2mM KCl, 0.5mM MgCl2-6H2O,0.5mM HEPES,0.6mM CaCl2,pH
7.6) it is configured to the perfusate of final concentration of 10-4mol/L;Wherein, 13 kinds of plant volatiles are successively are as follows: cinnamic acid, laurene,
Myrte, piperitone, methyl benzoate, 4- ethyl acetophenone, 4- methoxy benzyl alcohol, eugenol, trans- 3- hexenol,
N-octyl alcohol, 1-OCOL, cis- 3- hexenyl acetate, geranyl acetate.
3.3 express the egg mother cell of HarmOR59 to above-mentioned 13 kinds of plant volatiles using Two-electrode voltage-clamp system measurement
Current value, 4-8 cell of every kind of compound determination.The result shows that HarmOR59 can be by cinnamic acid strong activation, 10-4M is dense
Wherein maximum reacting value reaches 1000nA to the lower reaction average value 700nA of degree, remaining has the compound of reaction 10-4It is as follows under M concentration:
Methyl phenylacetate (283nA), 4- ethyl acetophenone (310nA), trans- 3- hexenol (364nA), n-octyl alcohol (367nA).
The EC of the egg mother cell of HarmOR59 is expressed in the activation such as 3.4 cinnamic acids50
Cinnamic acid under various concentration, methyl phenylacetate, 4- ethyl acetophenone, trans- 3- hexenol, n-octyl alcohol is measured respectively to swash
The response value of receptor living, and dose-response curve is drawn, calculate EC50Value.The result shows that HarmOR59 has highest clever cinnamic acid
Sensitivity, EC50Value is 1.716e-006mol/L.To the EC of other compounds50Value is respectively as follows: methyl phenylacetate (2.826e-
004mol/L), 4- ethyl acetophenone (7.246e-005mol/L), trans- 3- hexenol (4.945e-005mol/L), n-octyl alcohol
(4.116e-004mol/L)。
Embodiment 2
The preparation of attractant mother liquor:
It is adjacent to draw 50 μ L cinnamic acids (Cinnamaldehyde), 20 μ L salicylides (Salicylaldehyde), 10 μ L 2-
Methoxyl methyl benzoate (Methyl 2-methoxybenzoate), 10 μ L linalools (Linalool), 10 μ L limonenes
((R)-(+)-Limonene) is uniformly mixed.Attractant mother liquor after being made can be put into refrigerator preservation, and storage temperature is -22
℃。
The preparation of attractant:
Attractant mother liquor n-hexane is pressed 1000 times of gradient dilution, obtain the cinnamic acid of 0.5v ‰, ‰ salicylide of 0.2v,
The attractant of the limonene of the 2- methyl o-anisate of 0.2v ‰, the linalool of 0.1v ‰ and 0.1v ‰.
Using solvent hexane as negative control.
Comparative example 1
Compare the preparation of attractant mother liquor:
Draw 20 μ L salicylides (Salicylaldehyde), 10 μ L 2- methyl o-anisate (Methyl 2-
Methoxybenzoate), 10 μ L linalools (Linalool), 10 μ L limonenes ((R)-(+)-Limonene), use n-hexane
(Hexane) 100 μ L are settled to.It is uniformly mixed.Comparison attractant mother liquor after being made can be put into refrigerator preservation, storage temperature
It is -22 DEG C.
Compare the preparation of attractant:
1000 times of gradient dilution are pressed with n-hexane in use, attractant mother liquor will be compared, is uniformly mixed.Obtain ‰ water of 0.2v
Poplar aldehyde, the 2- methyl o-anisate of 0.2v ‰, the linalool of 0.1v ‰ and 0.1v ‰ limonene attractant.
Using solvent hexane as negative control.
Embodiment 3
Lure experiment
Indoor Y tube trapping experiment is carried out to attractant.
Place: test carries out in Plant Protection institute, Chinese Academy of Agricultral Sciences laboratory.
Time: test period is evening 18:00 to morning 2:00.
Indoor conditions: 26~28 DEG C of temperature, humidity 30%~50%;Light impacts action selection in order to prevent,
Use feux rouges as light source.
Instrument and equipment and setting: Y tube is glass material, and 60 ° of two side arms A, B angle, long 20cm, straight-arm are long respectively
25cm, three arm diameters are 4cm, and two side arm openings are clogged with the rubber stopper that centre is inserted with sebific duct respectively;Air-flow is big
After gas sampling instrument is collected, tower, Zhi Houfen are moistened by the glass drying tower equipped with active carbon and the glass equipped with pure water respectively
Stream enters two-arm, air velocity 1L/min.
Behaviouristics measurement:
(1) 50 μ L of prepared attractant (attractant of embodiment 1 or the comparison attractant of comparative example 1) are drawn to be added drop-wise to
On the rectangular filter paper of side length 1cm, wait the scraps of paper are put into A arm after the solvent is volatilized within 10 seconds, B arm place be added dropwise 50 μ L just oneself
The scraps of paper of alkane are as negative control;
(2) bollworm female, the male moth single head sprouted wings 1~2 day are put into straight-arm inlet, open atmosphere sampling instrument make air-flow into
Enter, starts simultaneously at timing, cotton bollworm moth is respectively in the residence time of two-arm in statistics 10min;
(3) Y tube and filter paper are replaced after every cotton bollworm moth test, and replaces the left-right position of A, B two-arm, at every group
In Behavior Test, the borer population that female, male moth uses is respectively 50.
Interpretation of result:
It counts female, male moth respectively to be inclined to the selection of different attractants, the individual of selection is not done in removal, is calculated and is selected each arm
Polypide number Zhan always select the percentage of polypide number, calculation formula are as follows: lure rate=selection attractant number of individuals/(selection is lured
Agent number of individuals+selection n-hexane number of individuals).The result shows that: the attractant of embodiment 2 is 58.8% to the rate of luring of female moth, right
The rate of luring of male moth is 66.6%;And comparative example 1 is 37% to the rate of luring of female moth, the rate of luring to male moth is 40.7%.It is right
Ratio 1 only lacks cinnamic acid compared with the formula of embodiment 2, shows that cinnamic acid plays a significant role in luring agent composition.
The attractant effect to bollworm female moth that the attractant of embodiment 2 obtains is higher than the attractant of comparative example 1 to bollworm female moth
Attractant effect 21.8%;The attractant effect to bollworm hero moth that the attractant of embodiment 2 obtains is higher than the attractant of comparative example 1
To the attractant effect 25.9% of bollworm hero moth.And then it concludes that the attractant containing cinnamic acid has and preferably lures work
With.
Sequence table
<110>Plant Protection institute, Chinese Academy of Agricultral Sciences
<120>a kind of attractant and its application in bollworm is being lured
<130> LHA1760771
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<213>bollworm (Helicoverpa armigera)
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atgtcgtcgt cagaccatga tatgttctac ttgatgtaca gtaatacagt caagttggcc 180
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<213>bollworm (Helicoverpa armigera)
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<212> DNA/RNA
<213>artificial sequence (non)
<400> 7
aagcttgctt gttctttttg cagaagctca gaaacgctca acttggcaga tctgccacca 60
tgggcttcgt cagaaatttc tggaaaaaat tgactcacac aaaggccctc gaccaatcaa 120
gtgggagatt ggagacagtt ttctttgagt ctatctatag gattacttat gtggcaggta 180
tgtcgtcgtc agaccatgat atgttctact tgatgtacag taatacagtc aagttggcca 240
tagtgtttct agtatgtgga gaaatatggt acgggttcac cgaagcttcg ggattggatg 300
aggtagctgc ctctatcaac gtcacggtca tacagtatat tgccatgtac agatttatga 360
acatgatgtc acataaggac ttttacaaga aattagcgac gtctatggaa tccccttatt 420
ttgatattac aacagaagaa agaaagaaac tggtggacta ttggtggcag actaacgaga 480
gatatttaaa gcttttgctt gctttgggaa actgcactct tgccttctgg ttcatcttcc 540
ccctagtcga cgatgtggac tacaacctca tagtggggat ccggctgcct ctaaactaca 600
aaaccccatt ccgataccct ttagcataca tagtggtgat gatagccttt ttctatatct 660
ctcattttgt gatgatcacg gatctgaaga tgcagacaca tttactgcat ctgctgtgcc 720
agtttacagt gctggtggat tgcttccaga atttgttgcg agattgtcgg attgggtttg 780
aagatgtagc ggaaaacaac ttagtttacg aaaaacgttt tgcagacaaa tacacaaagc 840
gacttggcga cctcgtggaa caacataaac ttattttgag caacacaatg aatttgcgcg 900
acacgctaag tagcccgatg ttgggacagc tggttgcgag cggtatactc atttgtttca 960
tcggttatca ggccactacg accatagcag aaagcccttt tcaaggtcta atgagtgctt 1020
tctttttggg ctacaatctt tttggtttct atataatttg ccgctggggt gaggagataa 1080
caaatcagag tgaaaagata ggagaagcca tatattgttc aggatgggaa tgtgggctag 1140
ccaaactacc tggagtacgg tccaccatca tgtacgtgat agcgagggcc aacaaacctc 1200
tggtcctcac cgcgggaggg atgtacaacc tctcactcac ctcttacact agtttggtga 1260
aaacatctta cagtgcgttg accgtattgc ttcaatttcg acacgaatag actagtgact 1320
gactaggatc tggttaccac taaaccagcc tcaagaacac ccgaatggag tctctaagct 1380
acataatacc aacttacact tacaaaatgt tgtcccccaa aatgtagcca ttcgtatctg 1440
ctcctaataa aaagaaagtt tcttcacatt ctaaaaaaaa aaaaaaaaaa aaaaaaaaaa 1500
aacccccccc cccccccccc cccccccccc cc 1532
<210> 8
<211> 59
<212> DNA/RNA
<213>artificial sequence (non)
<400> 8
aagcttgctt gttctttttg cagaagctca gaaacgctca acttggcaga tctgccacc 59
<210> 9
<211> 222
<212> DNA/RNA
<213>artificial sequence (non)
<400> 9
actagtgact gactaggatc tggttaccac taaaccagcc tcaagaacac ccgaatggag 60
tctctaagct acataatacc aacttacact tacaaaatgt tgtcccccaa aatgtagcca 120
ttcgtatctg ctcctaataa aaagaaagtt tcttcacatt ctaaaaaaaa aaaaaaaaaa 180
aaaaaaaaaa aacccccccc cccccccccc cccccccccc cc 222
<210> 10
<211> 1422
<212> DNA/RNA
<213>bollworm (Helicoverpa armigera)
<400> 10
atgatgacca aggtgaaggc ccagggcctc gtgtcagact tgatgcccaa catcaagctg 60
atgcagatgg ccggccattt cctcttcaat taccactcag aaaatgctgg catgtcaaac 120
cttctccgta agatctacgc gagtactcat gctatcctca ttttcatcca ctacgcgtgt 180
atgggaatca acatggcgaa atactccgat gaggtcaacg agctgacagc caataccata 240
actgtcttat tcttcgccca tactatcatc aagcttgcgt tctttgcttt aaactctaag 300
agcttctaca ggaccctggc agtatggaac cagtcgaaca gtcacccact gttcacggag 360
tcagacgctc gctatcacca gatcgcgctc accaagatga ggaggctgct gtacttcatc 420
tgcgggatga ctgtcctctc tgttatcagc tgggtaaccc taacattctt cggtgagtca 480
gtacgcatgg tgacgaacaa ggaaaccaac gagaccctga cggaggtggt gccccgcctg 540
cctctgaagg cctggtaccc ttttaatgct atgagcggca ctatgtatat cgtggcgttc 600
gcttttcagg tatactggct cctattctca atggccatag cgaacctgat ggacgtgatg 660
ttctgttcct ggctgatctt cgcgtgtgaa cagctgcagc atttgaaggc tatcatgaaa 720
cccctcatgg agttgagcgc ctctttggac acgtaccggc ctaatactgc tgagctgttt 780
agagcttctt ctactgagaa atccgaaaag atccccgaca cggtagacat ggacattcgc 840
ggcatatact ccacacagca agacttcggc atgacactcc gaggtgctgg tgggagactc 900
cagaacttcg gccagcagaa ccccaaccct aacggcttga ctcccaagca ggagatgctg 960
gccaggtctg ctatcaagta ctgggtggag aggcataagc atgtcgtcag actagtggca 1020
tcgattggag acacgtatgg taccgccctg ctgttccaca tgttggtgtc tacgatcaca 1080
ctcaccctgc tggcctacca agctactaag atcaacggaa tcaacgtgta tgctttcagt 1140
acgatcggat acttgagtta cactctgggt caagtgttcc acttctgcat ctttggaaat 1200
aggctcattg aagagagctc atcagtaatg gaagcagctt actcctgcca gtggtatgac 1260
ggctccgagg aagcgaagac attcgtgcaa atcgtctgcc aacagtgtca gaaggctatg 1320
agcatctccg gagccaagtt cttcacggtc tcacttgatt tgtttgcttc ggtccttgga 1380
gccgtggtaa cctacttcat ggtattgata cagctcaagt ga 1422
<210> 11
<211> 473
<212> PRT
<213>bollworm (Helicoverpa armigera)
<400> 11
Met Met Thr Lys Val Lys Ala Gln Gly Leu Val Ser Asp Leu Met Pro
1 5 10 15
Asn Ile Lys Leu Met Gln Met Ala Gly His Phe Leu Phe Asn Tyr His
20 25 30
Ser Glu Asn Ala Gly Met Ser Asn Leu Leu Arg Lys Ile Tyr Ala Ser
35 40 45
Thr His Ala Ile Leu Ile Phe Ile His Tyr Ala Cys Met Gly Ile Asn
50 55 60
Met Ala Lys Tyr Ser Asp Glu Val Asn Glu Leu Thr Ala Asn Thr Ile
65 70 75 80
Thr Val Leu Phe Phe Ala His Thr Ile Ile Lys Leu Ala Phe Phe Ala
85 90 95
Leu Asn Ser Lys Ser Phe Tyr Arg Thr Leu Ala Val Trp Asn Gln Ser
100 105 110
Asn Ser His Pro Leu Phe Thr Glu Ser Asp Ala Arg Tyr His Gln Ile
115 120 125
Ala Leu Thr Lys Met Arg Arg Leu Leu Tyr Phe Ile Cys Gly Met Thr
130 135 140
Val Leu Ser Val Ile Ser Trp Val Thr Leu Thr Phe Phe Gly Glu Ser
145 150 155 160
Val Arg Met Val Thr Asn Lys Glu Thr Asn Glu Thr Leu Thr Glu Val
165 170 175
Val Pro Arg Leu Pro Leu Lys Ala Trp Tyr Pro Phe Asn Ala Met Ser
180 185 190
Gly Thr Met Tyr Ile Val Ala Phe Ala Phe Gln Val Tyr Trp Leu Leu
195 200 205
Phe Ser Met Ala Ile Ala Asn Leu Met Asp Val Met Phe Cys Ser Trp
210 215 220
Leu Ile Phe Ala Cys Glu Gln Leu Gln His Leu Lys Ala Ile Met Lys
225 230 235 240
Pro Leu Met Glu Leu Ser Ala Ser Leu Asp Thr Tyr Arg Pro Asn Thr
245 250 255
Ala Glu Leu Phe Arg Ala Ser Ser Thr Glu Lys Ser Glu Lys Ile Pro
260 265 270
Asp Thr Val Asp Met Asp Ile Arg Gly Ile Tyr Ser Thr Gln Gln Asp
275 280 285
Phe Gly Met Thr Leu Arg Gly Ala Gly Gly Arg Leu Gln Asn Phe Gly
290 295 300
Gln Gln Asn Pro Asn Pro Asn Gly Leu Thr Pro Lys Gln Glu Met Leu
305 310 315 320
Ala Arg Ser Ala Ile Lys Tyr Trp Val Glu Arg His Lys His Val Val
325 330 335
Arg Leu Val Ala Ser Ile Gly Asp Thr Tyr Gly Thr Ala Leu Leu Phe
340 345 350
His Met Leu Val Ser Thr Ile Thr Leu Thr Leu Leu Ala Tyr Gln Ala
355 360 365
Thr Lys Ile Asn Gly Ile Asn Val Tyr Ala Phe Ser Thr Ile Gly Tyr
370 375 380
Leu Ser Tyr Thr Leu Gly Gln Val Phe His Phe Cys Ile Phe Gly Asn
385 390 395 400
Arg Leu Ile Glu Glu Ser Ser Ser Val Met Glu Ala Ala Tyr Ser Cys
405 410 415
Gln Trp Tyr Asp Gly Ser Glu Glu Ala Lys Thr Phe Val Gln Ile Val
420 425 430
Cys Gln Gln Cys Gln Lys Ala Met Ser Ile Ser Gly Ala Lys Phe Phe
435 440 445
Thr Val Ser Leu Asp Leu Phe Ala Ser Val Leu Gly Ala Val Val Thr
450 455 460
Tyr Phe Met Val Leu Ile Gln Leu Lys
465 470
<210> 12
<211> 1703
<212> DNA/RNA
<213>artificial sequence (non)
<400> 12
aagcttgctt gttctttttg cagaagctca gaaacgctca acttggcaga tctgccacca 60
tgatgaccaa ggtgaaggcc cagggcctcg tgtcagactt gatgcccaac atcaagctga 120
tgcagatggc cggccatttc ctcttcaatt accactcaga aaatgctggc atgtcaaacc 180
ttctccgtaa gatctacgcg agtactcatg ctatcctcat tttcatccac tacgcgtgta 240
tgggaatcaa catggcgaaa tactccgatg aggtcaacga gctgacagcc aataccataa 300
ctgtcttatt cttcgcccat actatcatca agcttgcgtt ctttgcttta aactctaaga 360
gcttctacag gaccctggca gtatggaacc agtcgaacag tcacccactg ttcacggagt 420
cagacgctcg ctatcaccag atcgcgctca ccaagatgag gaggctgctg tacttcatct 480
gcgggatgac tgtcctctct gttatcagct gggtaaccct aacattcttc ggtgagtcag 540
tacgcatggt gacgaacaag gaaaccaacg agaccctgac ggaggtggtg ccccgcctgc 600
ctctgaaggc ctggtaccct tttaatgcta tgagcggcac tatgtatatc gtggcgttcg 660
cttttcaggt atactggctc ctattctcaa tggccatagc gaacctgatg gacgtgatgt 720
tctgttcctg gctgatcttc gcgtgtgaac agctgcagca tttgaaggct atcatgaaac 780
ccctcatgga gttgagcgcc tctttggaca cgtaccggcc taatactgct gagctgttta 840
gagcttcttc tactgagaaa tccgaaaaga tccccgacac ggtagacatg gacattcgcg 900
gcatatactc cacacagcaa gacttcggca tgacactccg aggtgctggt gggagactcc 960
agaacttcgg ccagcagaac cccaacccta acggcttgac tcccaagcag gagatgctgg 1020
ccaggtctgc tatcaagtac tgggtggaga ggcataagca tgtcgtcaga ctagtggcat 1080
cgattggaga cacgtatggt accgccctgc tgttccacat gttggtgtct acgatcacac 1140
tcaccctgct ggcctaccaa gctactaaga tcaacggaat caacgtgtat gctttcagta 1200
cgatcggata cttgagttac actctgggtc aagtgttcca cttctgcatc tttggaaata 1260
ggctcattga agagagctca tcagtaatgg aagcagctta ctcctgccag tggtatgacg 1320
gctccgagga agcgaagaca ttcgtgcaaa tcgtctgcca acagtgtcag aaggctatga 1380
gcatctccgg agccaagttc ttcacggtct cacttgattt gtttgcttcg gtccttggag 1440
ccgtggtaac ctacttcatg gtattgatac agctcaagtg aactagtgac tgactaggat 1500
ctggttacca ctaaaccagc ctcaagaaca cccgaatgga gtctctaagc tacataatac 1560
caacttacac ttacaaaatg ttgtccccca aaatgtagcc attcgtatct gctcctaata 1620
aaaagaaagt ttcttcacat tctaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaccccccc 1680
cccccccccc cccccccccc ccc 1703
<210> 13
<211> 21
<212> DNA
<213>artificial sequence (non)
<400> 13
atgatgacca aggtgaaggc c 21
<210> 14
<211> 22
<212> DNA
<213>artificial sequence (non)
<400> 14
ttacttgagc tgtatcaata cc 22
<210> 15
<211> 47
<212> DNA
<213>artificial sequence (non)
<400> 15
acgctcaact tggcagatct gccaccatga tgaccaaggt gaaggcc 47
<210> 16
<211> 42
<212> DNA
<213>artificial sequence (non)
<400> 16
gatcctagtc agtcactagt ttacttgagc tgtatcaata cc 42
Claims (7)
1. a kind of attractant comprising solvent, cinnamic acid, salicylide, 2- methyl o-anisate, linalool and lemon
Alkene.
2. attractant according to claim 1, which is characterized in that by volume, the cinnamic acid containing in attractant
Amount is 0.1 ‰ to 1 ‰;Content of the salicylide in attractant is 0.1 ‰ to 0.5 ‰;The 2- o-methoxybenzoic acid
Content of the methyl esters in attractant is 0.1 ‰ to 0.5 ‰;Content of the linalool in attractant is 0.05 ‰ to 0.2 ‰;
Content of the limonene in attractant is 0.05 ‰ to 0.2 ‰.
3. attractant according to claim 2, which is characterized in that by volume, the cinnamic acid containing in attractant
Amount is 0.3 ‰ to 0.8 ‰;Content of the salicylide in attractant is 0.1 ‰ to 0.5 ‰;The 2- O-methoxy benzene first
Content 0.1 ‰ to 0.5 ‰ of the sour methyl esters in attractant;Content of the linalool in attractant is 0.05 ‰ to 0.2 ‰;
Content of the limonene in attractant is 0.05 ‰ to 0.2 ‰.
4. attractant according to claim 3, which is characterized in that by volume, the cinnamic acid containing in attractant
Amount is 0.3 ‰ to 0.8 ‰;Content of the salicylide in attractant is 0.1 ‰ to 0.3 ‰;The linalool is in attractant
In content be 0.1 ‰ to 0.3 ‰;Content 0.05 ‰ to 0.1 ‰ of the 2- methyl o-anisate in attractant;
Content of the limonene in attractant is 0.05 ‰ to 0.1 ‰.
5. attractant according to claim 4, which is characterized in that by volume, the cinnamic acid containing in attractant
Amount is 0.5 ‰;Content of the salicylide in attractant is 0.2 ‰;The 2- methyl o-anisate is in attractant
In content 0.2 ‰;Content of the linalool in attractant is 0.1 ‰;Content of the limonene in attractant be
0.1‰。
6. attractant as claimed in any of claims 1 to 5, which is characterized in that the solvent is selected from n-hexane, stone
At least one of wax oil, mineral oil.
7. attractant as claimed in any of claims 1 to 6 is for luring the application in bollworm.
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