CN109946825A - A kind of bidirectional imaging system for embryo, egg mother cell and stem cell - Google Patents
A kind of bidirectional imaging system for embryo, egg mother cell and stem cell Download PDFInfo
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- CN109946825A CN109946825A CN201910117910.0A CN201910117910A CN109946825A CN 109946825 A CN109946825 A CN 109946825A CN 201910117910 A CN201910117910 A CN 201910117910A CN 109946825 A CN109946825 A CN 109946825A
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- embryo
- egg mother
- stem cell
- pallet
- light source
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Abstract
The embodiment of the invention discloses a kind of bidirectional imaging systems for embryo, egg mother cell and stem cell, comprising: pallet is provided with several recess for carrying embryo, egg mother cell or stem cell；Optical devices are taken pictures for pallet, including first light source, the first beam condensing unit, the first object lens, second light source, the second beam condensing unit, the second object lens, the first charge coupling device imaging sensor and the second charge coupling device imaging sensor.Compared with prior art, it include two sets of light sources, beam condensing unit and object lens the invention has the following beneficial effects: a kind of bidirectional imaging system for embryo, egg mother cell and stem cell of the invention, it can take pictures simultaneously to sample from both direction, effectively overcome the image defects that single direction is taken pictures, the form of multiple embryos, egg mother cell or stem cell is more comprehensively presented, so that evaluation is more accurate.
Description
Technical field
The present invention relates to a kind of biotechnology medical auxiliary equipments, more particularly to one kind for embryo, egg mother cell and to do
The bidirectional imaging system of cell.
Background technique
In Past 30 Years, assisted reproductive technology (ART) revolutionizes the treatment of the mankind sterile (infertility),
And become universal.However, ART is needed using multiple embryos, multiple embryos result in frequently-occurring multifetation, thus
So that the death rate and pain of baby and mother greatly increase, and generate very big economic cost.
The low success rate of one of the main reasons of ART is to lack the method for evaluating embryo quality.It uses at present
In prediction embryo quality most reliable method be before transfer using standard transmitted light microscopic system to embryo morphology into
Capable inspection of taking pictures.It is usually taken pictures to embryo in one direction to evaluate the quality of the embryo, but since embryo is one
Ball-type is only taken pictures from a direction, can not comprehensively show the form of embryo, or even embryonic cell occur mutual in this direction
Overlapping, the problem of influencing each other.
Summary of the invention
It is an object of the invention to overcome above-mentioned technical deficiency, propose a kind of for embryo, egg mother cell and stem cell
Bidirectional imaging system, solution in the prior art only take pictures to embryo from a direction, can not comprehensively show the shape of embryo
The technical issues of state.
In order to reach above-mentioned technical purpose, the embodiment of the invention provides one kind to be used for embryo, egg mother cell and stem cell
Bidirectional imaging system, which includes:
Pallet is provided with several recess for carrying embryo, egg mother cell or stem cell；
Optical devices are taken pictures for pallet, including first light source, the first beam condensing unit, the first object lens, the second light
Source, the second beam condensing unit, the second object lens, the first charge coupling device imaging sensor and the second charge coupling device image pass
Sensor, the first beam condensing unit, pallet, the first object lens and the first charge coupling device imaging sensor are successively set on
On the optical path of one light source, the second beam condensing unit, pallet, the second object lens and the second charge coupling device image sensing
Device is successively set on the optical path of second light source.
Compared with prior art, the invention has the following beneficial effects: one kind of the invention for embryo, egg mother cell and
The bidirectional imaging system of stem cell include two sets light sources, beam condensing unit and object lens, can from both direction and meanwhile to sample into
Row is taken pictures, and the image defects that single direction is taken pictures effectively are overcome, and multiple embryos, egg mother cell or dry thin are more comprehensively presented
The form of born of the same parents, so that evaluation is more accurate.
Detailed description of the invention
Fig. 1 is that a kind of structure of bidirectional imaging system for embryo, egg mother cell and stem cell provided by the invention is shown
It is intended to；
Fig. 2 is the structure of another bidirectional imaging system for embryo, egg mother cell and stem cell provided by the invention
Schematic diagram；
Fig. 3 is the structural schematic diagram of pallet in first embodiment；
Fig. 4 is the structural schematic diagram of pallet in second embodiment.
Specific embodiment
Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, complete
Site preparation description.
Referring to Figure 1 to Fig. 4, Fig. 1 be it is provided by the invention it is a kind of for embryo, egg mother cell and stem cell it is two-way at
As the structural schematic diagram of system；Fig. 2 is provided by the invention another for embryo, the bidirectional imaging of egg mother cell and stem cell
The structural schematic diagram of system；Fig. 3 is the structural schematic diagram of pallet in first embodiment；Fig. 4 is loading in second embodiment
The structural schematic diagram of pallet.
A kind of bidirectional imaging system for embryo, egg mother cell and stem cell includes: pallet 1 and optics dress
It sets.The present invention couples for the imaging system of embryo, egg mother cell or stem cell with outer computer, so as to the calculating
Machine communication, and there is the software for analyzing the image obtained by the imaging system in the computer.
Pallet 1 is linear type, is provided with several recess for carrying embryo, egg mother cell or stem cell, is recessed
It is arranged successively the type of being in line.Optical devices may be mounted in linear guide and move, successively to the embryo of carrying, egg mother cell or
Stem cell is taken pictures respectively.For the refraction generated when reducing light across pallet 1, the cross section of pallet 1 is at least wanted
Including a pair of orthogonal side wall.In example 1, the cross section of pallet 1 is right angled triangle；In embodiment two
In, the cross section of pallet 1 is rectangle.
Optical devices are the equipment taken pictures for embryo, egg mother cell or the stem cell in pallet 1, including first
Light source 21, the first beam condensing unit 22, the first object lens 23, first refractive device 24, the first charge coupling device imaging sensor 25,
Second light source 31, the second beam condensing unit 32, the second object lens 33, the second refracting means 34 and the second charge coupling device image pass
Sensor 35.First light source 21 and second light source 31 are irradiated towards pallet 1, and light is in 90 ° of angles, at the same also respectively with
The orthogonal two side walls of pallet 1 are respectively perpendicular, with what is generated when reducing light as far as possible across 1 outer wall of pallet
Refracted light.
Along the optical path of first light source 21, set gradually the first beam condensing unit 22, pallet 1, the first object lens 23,
First refractive device 24 and the first charge coupling device imaging sensor 25, first refractive device 24 generate first light source 21
Light refraction to the photosurface of the first charge coupling device imaging sensor 25 on.Along the optical path of second light source 31,
Set gradually the second beam condensing unit 32, pallet 1, the second object lens 33, the second refracting means 34 and the second charge coupling apparatus
Part imaging sensor 35, the light refraction that the second refracting means 24 generate second light source 31 to the second charge coupling device image
On the photosurface of sensor 35.
The layout drawing of two kinds of refracting means is each provided in Fig. 1 and Fig. 2, but the arrangement of refracting means is not limited only to
Both.It is to be appreciated that entire imaging system can also be not provided with refracting means, the setting of refracting means is to change light
Road is to adapt to the layout of charge coupling device imaging sensor.
In the present embodiment, the first beam condensing unit 22 and the second beam condensing unit 32 use condenser, for respectively to first
The light that light source 21 and second light source 31 generate is converged, and is pooled in fact a bit, imaging is more clear.
In order to more accurately focus when taking pictures, pallet 1 of the invention and Optical devices are arranged in one
On a displacement mechanism, opposing optical device on tri- directions XYZ of the pallet 1 on conversational implication is enabled to carry out position
It moves, which includes the high precision displacement under fast offset and small scale under large scale, generallys use chain, liquid
The common displacement mechanism combination such as pressure transmission is realized.Its purpose is to along pallet 1 be recessed orientation campaign, successively to load
Multiple groups embryo, egg mother cell or stem cell in object pallet 1 are carried out continuously high-precision and take pictures, to obtain multiple groups in a short time
A large amount of high definition photos in embryo, egg mother cell or stem cell both direction.
The implementation of the embodiments of the present invention has the following beneficial effects: one kind of the invention is for embryo, egg mother cell and does thin
The bidirectional imaging system of born of the same parents includes two sets of light sources, beam condensing unit and object lens, can be clapped simultaneously to sample from both direction
According to effectively overcoming the image defects that single direction is taken pictures, multiple embryos, egg mother cell or stem cell be more comprehensively presented
Form, so that evaluation is more accurate.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all in essence of the invention
Within mind and principle, any modification, equivalent replacement, improvement and so on be should all be included in the protection scope of the present invention.
Claims (7)
1. a kind of bidirectional imaging system for embryo, egg mother cell and stem cell, characterized in that it comprises:
Pallet is provided with several recess for carrying embryo, egg mother cell or stem cell；
Optical devices are taken pictures for the pallet, including first light source, the first beam condensing unit, the first object lens, the second light
Source, the second beam condensing unit, the second object lens, the first charge coupling device imaging sensor and the second charge coupling device image pass
Sensor, first beam condensing unit, the pallet, first object lens and the first charge coupling device image pass
Sensor is successively set on the optical path of the first light source, second beam condensing unit, the pallet, described second
Object lens and the second charge coupling device imaging sensor are successively set on the optical path of the second light source.
2. a kind of bidirectional imaging system for embryo, egg mother cell and stem cell according to claim 1, feature exist
In the first light source and the second light source are in 90 ° of angles.
3. a kind of bidirectional imaging system for embryo, egg mother cell and stem cell according to claim 2, feature exist
In the pallet is linear type, and the recess is arranged successively.
4. a kind of bidirectional imaging system for embryo, egg mother cell and stem cell according to claim 3, feature exist
In the pallet cross section is right angled triangle or rectangle.
5. a kind of bidirectional imaging system for embryo, egg mother cell and stem cell according to claim 4, feature exist
In the orthogonal two side walls of pallet are respectively perpendicular to the optical path and second light of the first light source
The optical path in source is arranged.
6. a kind of bidirectional imaging system for embryo, egg mother cell and stem cell according to claim 5, feature exist
In the Optical devices further include first refractive device, and first refractive device setting is in first object lens and described the
Between one charge coupling device imaging sensor.
7. a kind of bidirectional imaging system for embryo, egg mother cell and stem cell according to claim 6, feature exist
In the Optical devices further include the second refracting means, and second refracting means setting is in second object lens and described the
Between two charge coupling device imaging sensors.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910117910.0A CN109946825A (en) | 2019-02-15 | 2019-02-15 | A kind of bidirectional imaging system for embryo, egg mother cell and stem cell |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910117910.0A CN109946825A (en) | 2019-02-15 | 2019-02-15 | A kind of bidirectional imaging system for embryo, egg mother cell and stem cell |
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| Publication Number | Publication Date |
|---|---|
| CN109946825A true CN109946825A (en) | 2019-06-28 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201910117910.0A Pending CN109946825A (en) | 2019-02-15 | 2019-02-15 | A kind of bidirectional imaging system for embryo, egg mother cell and stem cell |
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Citations (6)
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| CN104502255A (en) * | 2014-12-29 | 2015-04-08 | 中国科学院长春光学精密机械与物理研究所 | Three-dimensional imaging flow cytometer device |
| CN105496048A (en) * | 2016-01-27 | 2016-04-20 | 珠海格力电器股份有限公司 | Decoration strip, electronic product, household appliance and furniture |
| WO2017035078A1 (en) * | 2015-08-26 | 2017-03-02 | The United States Of America, As Represented By The Secretary, Department Of Health And Human Services | Systems and methods for multi-view nonlinear optical imaging for improved signal-to-noise ratio and resolution in point scanning multi-photon microscopy |
| CN106646699A (en) * | 2017-03-09 | 2017-05-10 | 索尔思光电（成都）有限公司 | Prism and light transmit-receive assembly |
| CN206248866U (en) * | 2016-12-07 | 2017-06-13 | 福州新三捷光电技术有限公司 | A kind of five jiaos of Amici prisms |
| CN107749936A (en) * | 2017-10-12 | 2018-03-02 | 武汉互创联合科技有限公司 | A kind of imaging system for embryo, egg mother cell or stem cell |
-
2019
- 2019-02-15 CN CN201910117910.0A patent/CN109946825A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104502255A (en) * | 2014-12-29 | 2015-04-08 | 中国科学院长春光学精密机械与物理研究所 | Three-dimensional imaging flow cytometer device |
| WO2017035078A1 (en) * | 2015-08-26 | 2017-03-02 | The United States Of America, As Represented By The Secretary, Department Of Health And Human Services | Systems and methods for multi-view nonlinear optical imaging for improved signal-to-noise ratio and resolution in point scanning multi-photon microscopy |
| CN105496048A (en) * | 2016-01-27 | 2016-04-20 | 珠海格力电器股份有限公司 | Decoration strip, electronic product, household appliance and furniture |
| CN206248866U (en) * | 2016-12-07 | 2017-06-13 | 福州新三捷光电技术有限公司 | A kind of five jiaos of Amici prisms |
| CN106646699A (en) * | 2017-03-09 | 2017-05-10 | 索尔思光电（成都）有限公司 | Prism and light transmit-receive assembly |
| CN107749936A (en) * | 2017-10-12 | 2018-03-02 | 武汉互创联合科技有限公司 | A kind of imaging system for embryo, egg mother cell or stem cell |
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Application publication date: 20190628 |