CN109932470A - The alkalization method of silica G plate in a kind of traditional Chinese medicine of licorice root preparation indentification by TLC - Google Patents
The alkalization method of silica G plate in a kind of traditional Chinese medicine of licorice root preparation indentification by TLC Download PDFInfo
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Abstract
The invention discloses a kind of alkalization methods of silica G plate in traditional Chinese medicine of licorice root preparation indentification by TLC, it is characterized in that, the following steps are included: step 1, the configuration of alkalizing solution: according to sodium hydrate solid: distilled water=1:100 ratio, weigh sodium hydrate solid xg, add distilled water dissolution to be settled to 100xml, that is, produces 1% sodium hydroxide solution；The alkalization method of silica G plate impregnates silica G plate 1 minute and naturally dry by using 1%NaOH solution in a kind of traditional Chinese medicine of licorice root preparation indentification by TLC of the invention, it is activated 15 minutes in 105 DEG C, compared with prior art, silica gel g thin-layer plate provided in the present invention impregnates alkalization method, it can effectively improve the chromatographic isolation degree of Radix Glycyrrhizae indentification by TLC, and time cost is saved, qualification result is accurate, is easy to observe；Therefore, method proposed by the invention can substitute prefabricated alkaline silica gel G lamellae, apply in the Radix Glycyrrhizae indentification by TLC of Chinese materia medica preparation.
Description
Technical field
The invention belongs to silica gel in medical detection technique field more particularly to a kind of traditional Chinese medicine of licorice root preparation indentification by TLC
The alkalization method of G plate.
Background technique
Radix Glycyrrhizae has and middle emergency, moistening lung, removing toxic substances, the characteristic of coordinating the drug actions of a prescription, make it frequently as make medicine for Chinese medicine compound prescription
In preparation prescription, therefore when establishing compound Chinese medicinal preparation quality standard, frequently as the quality for identifying one of item control preparation.
The main component of Radix Glycyrrhizae has flavonoids and saponins, such as: liquiritin and glycyrrhizic acid, is all to belong to the biggish chemical component of polarity；
Therefore the stationary phase required under the identification item of one Radix Glycyrrhizae of current edition pharmacopeia is that the silica G of 1% sodium hydroxide solution preparation is thin
Laminate, but the lamellae of this specification rarely has supply in the market.
Therefore the discrimination method of the related Radix Glycyrrhizae of the prior art is replaced using silica G plate, but in practical operation
In journey it was found that if substituted using common silica gel g thin-layer plate, due to silica gel thin-layer plate it is big for polarity chemistry at
The strong adsorption force divided causes chromatography spot separating degree poor so that chromatography spot seems larger wider because of hangover；If
Using hand-made lamellae, chromatographic isolation result can also silica gel thickness after by the granularity and preparation of used silica gel it is uniform
The influence of degree；So a kind of new method is needed to solve the problems, such as encountered in the identification of Radix Glycyrrhizae.
Summary of the invention
The present invention provides a kind of alkalization method of silica G plate in traditional Chinese medicine of licorice root preparation indentification by TLC, it is intended to solve existing
The discrimination method of the related Radix Glycyrrhizae of technology is replaced using silica G plate, but in the actual operation process it was found that such as
Fruit is substituted using common silica gel g thin-layer plate, due to the strong adsorption force of the silica gel thin-layer plate chemical component big for polarity, because
And chromatography spot is made to seem larger wider because of hangover, cause chromatography spot separating degree poor；If using hand-made
Lamellae, chromatographic isolation result can also by asked using what the silica gel thickness uniformity after the granularity and preparation of silica gel was influenced
Topic.
The invention is realized in this way in a kind of traditional Chinese medicine of licorice root preparation indentification by TLC silica G plate alkalization method, packet
Include following steps:
The configuration of alkalizing solution: step 1 takes a certain amount of sodium hydrate solid and distilled water to configure 1% sodium hydroxide
Solution；
Step 2, impregnate silica G plate: silica G plate to be processed is put into fill described in step 1 1% hydroxide
Dipping takes out silica G plate after 1 minute, 1 minute in the container of sodium solution；
Step 3, the activation of silica G plate: natural air drying after silica G plate described in step 2 is taken out, by the silicon after drying
Glue G plate is placed in 105 DEG C of heating plates to heat 15-60 minutes and activate；The silica G plate that step activated is put into spare in drier.
Preferably, the activation time of silica G plate is preferably 15 minutes in the step 3.
Compared with prior art, the beneficial effects of the present invention are: a kind of traditional Chinese medicine of licorice root preparation thin-layer chromatography of the invention reflects
The alkalization method of not middle silica G plate impregnates silica G plate 1 minute and naturally dry by using 1%NaOH solution, in 105 DEG C of work
Change 15 minutes, compared with prior art, silica gel g thin-layer plate provided in the present invention impregnates alkalization method, can effectively improve Radix Glycyrrhizae
The chromatographic isolation degree of indentification by TLC, and time cost is saved, qualification result is accurate, is easy to observe；Therefore, the present invention is mentioned
Method out can substitute prefabricated alkaline silica gel G lamellae, apply in the Radix Glycyrrhizae indentification by TLC of Chinese materia medica preparation.
It should be understood that the above is a preferred embodiment of the present invention, it is noted that for the art
For those of ordinary skill, various improvements and modifications may be made without departing from the principle of the present invention, these improvement
Also it is considered as protection scope of the present invention with retouching.
Detailed description of the invention
Fig. 1 is the schematic diagram of the unsoaked lamellae of the present invention；
Fig. 2 is the lamellae schematic diagram after impregnating under the different soaking times of the present invention；
Fig. 3 is the schematic diagram of lamellae of the present invention activation 15 minutes Radix Glycyrrhizae indentification by TLC of heating；
Fig. 4 is the schematic diagram of lamellae of the present invention activation 30 minutes Radix Glycyrrhizae indentification by TLC of heating；
Fig. 5 is the schematic diagram of lamellae of the present invention activation 60 minutes Radix Glycyrrhizae indentification by TLC of heating；
Fig. 6 is that pharynx health-care composition uses the signal of the Radix Glycyrrhizae indentification by TLC of optimization thin layer G plate in the embodiment of the present invention 3
Figure；
Fig. 7 is that pharynx health-care composition uses the signal of the Radix Glycyrrhizae indentification by TLC of common thin layer G plate in the embodiment of the present invention 3
Figure；
Fig. 8 is that reconnection oral solution uses the signal of the Radix Glycyrrhizae indentification by TLC of optimization thin layer G plate in the embodiment of the present invention 4
Figure；
Fig. 9 is compound ox Siberian cocklebur particle showing using the Radix Glycyrrhizae indentification by TLC for optimizing thin layer G plate in the embodiment of the present invention 5
It is intended to；
Specific embodiment
In order to make the objectives, technical solutions, and advantages of the present invention clearer, with reference to the accompanying drawings and embodiments, right
The present invention is further elaborated.It should be appreciated that the specific embodiments described herein are merely illustrative of the present invention, and
It is not used in the restriction present invention.
In the description of the present invention, it is to be understood that, term " length ", " width ", "upper", "lower", "front", "rear",
The orientation or positional relationship of the instructions such as "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outside" is based on attached drawing institute
The orientation or positional relationship shown, is merely for convenience of description of the present invention and simplification of the description, rather than the dress of indication or suggestion meaning
It sets or element must have a particular orientation, be constructed and operated in a specific orientation, therefore should not be understood as to limit of the invention
System.In addition, in the description of the present invention, the meaning of " plurality " is two or more, unless otherwise specifically defined.
Embodiment 1
The alkalization method of silica G plate in a kind of traditional Chinese medicine of licorice root preparation indentification by TLC, comprising the following steps:
Step 1, the configuration of alkalizing solution: according to sodium hydrate solid: distilled water=1:100 ratio weighs hydroxide
Sodium solid 10g adds distilled water dissolution to be settled to 1000ml, that is, produces 1% sodium hydroxide solution；
Step 2, impregnate silica G plate: silica G plate to be processed is put into fill described in step 1 1% hydroxide
Dipping takes out silica G plate after 1 minute, 1 minute in the container of sodium solution；
Step 3, the activation of silica G plate: natural air drying after silica G plate described in step 2 is taken out, by the silicon after drying
Glue G plate is placed in 105 DEG C of heating plates and heats 15-60 minutes；
Step 4, the drying of silica G plate: the silica G plate activated described in step 3 is put into drier, at one section
Between after by after drying silica G plate take out it is spare to get.
It is understood that according to above-mentioned steps, by 12 pieces of 5 × 10cm silica gel g thin-layer plates, as shown in Figure 1, divide four groups,
Number 1,2,3,4 respectively；It is respectively put into dipping in the container for the sodium hydroxide solution for filling 1%, it is contemplated that the thin layer after alkalization
Silica gel on plate is relatively thin, and soaking time is longer it is possible that the phenomenon that falling off, therefore dip time is respectively 1 minute, 2 points
Clock, 5 minutes, 10 minutes take out, dry, and observe.The result is shown in Figure 1-2, by Fig. 1 and Fig. 2 comparison it is found that only number is three pieces of 1
Lamellae is intact, and numbers the layer of silica gel on 9 blocks of lamellaes for being 2,3,4, has different degrees of cracking and falls off existing
As No. 2 most light, No. 4 most serious illustrate that soaking time cannot be too long, therefore the alkalization soaking time of silica G plate of the present invention is 1
Minute.
Embodiment 2
The alkalization method of silica G plate in a kind of traditional Chinese medicine of licorice root preparation indentification by TLC, comprising the following steps:
Step 1, the configuration of alkalizing solution: according to sodium hydrate solid: distilled water=1:100 ratio weighs hydroxide
Sodium solid 10g adds distilled water dissolution to be settled to 1000ml, that is, produces 1% sodium hydroxide solution；
Step 2, impregnate silica G plate: muti-piece silica G plate to be processed is put into fill described in step 1 1% hydrogen
Dipping takes out silica G plate after 1 minute, 1 minute in the container of sodium hydroxide solution；
Step 3, the activation of silica G plate: natural air drying after muti-piece silica G plate described in step 2 takes out, it will after drying
The silica G plate is respectively placed in 105 DEG C of heating plates and heats 15 minutes, 30 minutes and 60 minutes, and is marked；
Step 4, the drying of silica G plate: the silica G plate activated described in step 3 is put into drier, at one section
Between after by after drying silica G plate take out it is spare to get.
It is understood that needing to reflect using corresponding glycyrrhiza preparation after silica G plate is made according to above-mentioned steps
Not, to test the performance of silica G plate, steps are as follows for identification:
Step 1, the configuration of model drug test solution:
Taking the pharynx health-care composition containing Radix Glycyrrhizae, (lot number: 20170727) 10ml adds methanol 30ml, and ultrasound 30 minutes filters, filter
Liquid is evaporated, and residue adds water 20ml, makes to dissolve, and is added extracting n-butyl alcohol 2 times, each 20ml, is merged n-butanol liquid, is evaporated, adds methanol
1ml makes to dissolve, as test solution；
Step 2, the configuration of Radix Glycyrrhizae control medicinal material solution:
Extracting liquorice control medicinal material (lot number: 120904-200410) 1g, add diethyl ether 40ml, ultrasound 30 minutes, and filtration discards
Ether liquid, the dregs of a decoction add water 20ml, make to dissolve, and add extracting n-butyl alcohol 2 times, each 20ml, merge n-butanol liquid, are evaporated, add methanol 1ml
Make to dissolve, as control medicinal material solution；
Step 3, thin-layer chromatographic analysis:
According to thin-layered chromatography (four general rules 0502 of Chinese Pharmacopoeia version in 2015) test, draw above-mentioned 2 μ l of test solution,
1 μ l of control medicinal material solution, the silica G plate different to the above-mentioned heat-activated time carries out point sample respectively, with ethyl acetate: formic acid:
Glacial acetic acid: solvent is made in water=15:1:1:2 ratio, and the silica G plate after point sample is placed in corresponding vessel and is unfolded, and specifies
It takes out, dries after time, with 10% ethanol solution of sulfuric acid, it is clear to be heated to spot development at 105 DEG C for spray, sets ultraviolet lamp
It is inspected under (365nm)；
After the expansion of above-mentioned chromatographic condition point sample, using chromatographic isolation effect as index, the heat-activated time is investigated to silicon
The influence of glue G plate performance, is as a result shown in Fig. 3-5；Wherein Fig. 3 is 15 minutes Radix Glycyrrhizae indentification by TLC of heating as a result, Fig. 4 is heating
30 minutes Radix Glycyrrhizae indentification by TLC are as a result, Fig. 5 is 60 minutes Radix Glycyrrhizae indentification by TLC results of heating；It is understood that
Number respectively represents chromatographic identification corresponding to corresponding reagent as a result, in Fig. 3-Fig. 5 in figure: 1 is institute after test solution point sample
It obtains as a result, 2 be acquired results after Radix Glycyrrhizae control medicinal material solution point sample；
By analyzing Fig. 3-Fig. 5 acquired results, it is known that the chromatographic isolation effect of heating 15 minutes and 30 minutes connects
Closely, 1 hour chromatographic isolation effect is heated to be declined, it is contemplated that it saves time and cost, a kind of traditional Chinese medicine of licorice root system of the invention
The activation time that the alkalization method of silica G plate preferentially selects heating 15 minutes as silica G plate in agent indentification by TLC；
Embodiment 3
The above-mentioned experiment test solution of different batches causes unnecessary influence, the present embodiment to the present invention in order to prevent
Experimental result is verified again using the one species test solution of different batches, and use common silica G plate as
Control, concrete operations are as follows:
The alkalization method of silica G plate in a kind of traditional Chinese medicine of licorice root preparation indentification by TLC, comprising the following steps:
Step 1, the configuration of alkalizing solution: according to sodium hydrate solid: distilled water=1:100 ratio weighs hydroxide
Sodium solid 10g adds distilled water dissolution to be settled to 1000ml, that is, produces 1% sodium hydroxide solution；
Step 2, impregnate silica G plate: muti-piece silica G plate to be processed is put into fill described in step 1 1% hydrogen
Dipping takes out silica G plate after 1 minute, 1 minute in the container of sodium hydroxide solution；
Step 3, the activation of silica G plate: natural air drying after muti-piece silica G plate described in step 2 takes out, it will after drying
The silica G plate is respectively placed in 105 DEG C of heating plates and heats 15 minutes, is activated；
Step 4, the drying of silica G plate: the silica G plate activated described in step 3 is put into drier, at one section
Between after by after drying silica G plate take out it is spare to get.
It is understood that needing to reflect using corresponding glycyrrhiza preparation after silica G plate is made according to above-mentioned steps
Not, to test the performance of silica G plate, steps are as follows for identification:
Step 1, the configuration of model drug test solution:
Taking the pharynx health-care composition containing Radix Glycyrrhizae respectively, (lot number: 20170727,20170918,20171214) 10ml adds methanol
30ml, ultrasound 30 minutes, filtration, filtrate are evaporated, and residue adds water 20ml, makes to dissolve, and add extracting n-butyl alcohol 2 times, each 20ml, close
And n-butanol liquid, it is evaporated, adds methanol 1ml to make to dissolve, as test solution；
Step 2, the configuration of Radix Glycyrrhizae control medicinal material solution:
Extracting liquorice control medicinal material (lot number: 120904-200410) 1g, add diethyl ether 40ml, ultrasound 30 minutes, and filtration discards
Ether liquid, the dregs of a decoction add water 20ml, make to dissolve, and add extracting n-butyl alcohol 2 times, each 20ml, merge n-butanol liquid, are evaporated, add methanol 1ml
Make to dissolve, as control medicinal material solution.
Step 3, thin-layer chromatographic analysis:
It is tested according to thin-layered chromatography (four general rules 0502 of Chinese Pharmacopoeia version in 2015), draws above-mentioned different lot numbers respectively
2 μ l of test solution, 1 μ l of control medicinal material solution carry out point sample and label to above-mentioned processed silica G plate, with ethyl acetate:
Formic acid: glacial acetic acid: solvent is made in water=15:1:1:2 ratio, and the silica G plate after point sample is placed in corresponding vessel and is unfolded,
It takes out, dries after specified time, with 10% ethanol solution of sulfuric acid, it is clear to be heated to spot development at 105 DEG C for spray, sets ultraviolet light
It is inspected under lamp (365nm), it is as shown in Figure 6 to inspect result；
Step 4 reuses identical Radix Glycyrrhizae system after the silica G plate after above-mentioned optimization is replaced with common silica G plate
Agent is identify again by step 1 to three, and it is as shown in Figure 7 to inspect result.
It is understood that number respectively represents chromatographic identification corresponding to corresponding reagent as a result, in Fig. 6 and Fig. 7 in figure:
1,2,3 is test sample, and it is Radix Glycyrrhizae control medicinal material that lot number, which is respectively 20170727,20170918,20171214,4, and 5 be scarce Radix Glycyrrhizae
Negative control；It is as shown in Figure 7: using in the Radix Glycyrrhizae indentification by TLC figure in common silica G plate, sample chromatogram, with
On the corresponding position of reference medicine chromatography, the fluorescence principal spot of same color is shown, but since spot is too big, leads to chromatographic isolation
It spends excessively poor；And in Fig. 6, the dipping alkalization method of optimization is used, spot point is obviously reduced, and effectively improves chromatographic isolation
Degree；Therefore, this example demonstrates that in a kind of traditional Chinese medicine of licorice root preparation indentification by TLC of the invention silica G plate alkalization method institute
Silica G plate obtained has separating degree good, and the advantage that method is stable.
Embodiment 4
In order to further verify above-mentioned experimental result, 2015 editions pharmacopeia are combined with the dipping alkalization method after the above optimization
Thin-layer identification method under middle Radix Glycyrrhizae carries out Radix Glycyrrhizae thin layer to other preparations containing Radix Glycyrrhizae and identifies research, and process is as follows:
The alkalization method of silica G plate in a kind of traditional Chinese medicine of licorice root preparation indentification by TLC, comprising the following steps:
Step 1, the configuration of alkalizing solution: according to sodium hydrate solid: distilled water=1:100 ratio weighs hydroxide
Sodium solid 10g adds distilled water dissolution to be settled to 1000ml, that is, produces 1% sodium hydroxide solution；
Step 2, impregnate silica G plate: muti-piece silica G plate to be processed is put into fill described in step 1 1% hydrogen
Dipping takes out silica G plate after 1 minute, 1 minute in the container of sodium hydroxide solution；
Step 3, the activation of silica G plate: natural air drying after muti-piece silica G plate described in step 2 takes out, it will after drying
The silica G plate is respectively placed in 105 DEG C of heating plates and heats 15 minutes, is activated；
Step 4, the drying of silica G plate: the silica G plate activated described in step 3 is put into drier, at one section
Between after by after drying silica G plate take out it is spare to get.
It is understood that needing to reflect using corresponding glycyrrhiza preparation after silica G plate is made according to above-mentioned steps
Not, to test the performance of silica G plate, steps are as follows for identification:
Step 1, the configuration of model drug test solution:
Taking the reconnection oral solution containing Radix Glycyrrhizae respectively, (lot number: 20180216,20180428,20180601) 10ml adds first
Alcohol 30ml, ultrasound 30 minutes, filtration, filtrate are evaporated, and residue adds water 20ml, makes to dissolve, and add extracting n-butyl alcohol 2 times, each 20ml,
Merge n-butanol liquid, is evaporated, adds methanol 1ml to make to dissolve, as test solution；
Step 2, the configuration of Radix Glycyrrhizae control medicinal material solution:
Extracting liquorice control medicinal material (lot number: 120904-200410) 1.103g, add diethyl ether 40ml, ultrasound 30 minutes, filtration,
Ether liquid is discarded, the dregs of a decoction add water 20ml, make to dissolve, and add extracting n-butyl alcohol 2 times, each 20ml, merge n-butanol liquid, are evaporated, add first
Alcohol 1ml makes to dissolve, as control medicinal material solution；Negative solution 10ml is taken simultaneously, is made in the same way of negative control solution；
Step 3, thin-layer chromatographic analysis:
It is tested according to thin-layered chromatography (four general rules 0502 of Chinese Pharmacopoeia version in 2015), draws above-mentioned different lot numbers respectively
1 μ l of 2 μ l of test solution, 2 μ l of negative control solution and control medicinal material solution carries out point sample to above-mentioned processed silica G plate
And label, with ethyl acetate: formic acid: glacial acetic acid: solvent is made in water=15:1:1:2 ratio, by the silica G plate after point sample
It is placed in corresponding vessel and is unfolded, taken out after specified time, dried, spray with 10% ethanol solution of sulfuric acid, be heated to spot at 105 DEG C
Point colour developing is clear, sets and is inspected under ultraviolet lamp (365nm), it is as shown in Figure 8 to inspect result；
In Fig. 8: 1,2,3 is test sample, and it is that Radix Glycyrrhizae compares that lot number, which is respectively 20180216,20180428,20180601,4,
Medicinal material, 5 be the negative control for lacking Radix Glycyrrhizae；As shown in figure 8, in sample chromatogram, on position corresponding with reference medicine chromatography,
The fluorescence spot of aobvious same color, and negative control is noiseless.
Embodiment 5
In order to further verify above-mentioned experimental result, 2015 editions pharmacopeia are combined with the dipping alkalization method after the above optimization
Thin-layer identification method under middle Radix Glycyrrhizae carries out Radix Glycyrrhizae thin layer to other preparations containing Radix Glycyrrhizae and identifies research, and process is as follows:
The alkalization method of silica G plate in a kind of traditional Chinese medicine of licorice root preparation indentification by TLC, comprising the following steps:
Step 1, the configuration of alkalizing solution: according to sodium hydrate solid: distilled water=1:100 ratio weighs hydroxide
Sodium solid 10g adds distilled water dissolution to be settled to 1000ml, that is, produces 1% sodium hydroxide solution；
Step 2, impregnate silica G plate: muti-piece silica G plate to be processed is put into fill described in step 1 1% hydrogen
Dipping takes out silica G plate after 1 minute, 1 minute in the container of sodium hydroxide solution；
Step 3, the activation of silica G plate: natural air drying after muti-piece silica G plate described in step 2 takes out, it will after drying
The silica G plate is respectively placed in 105 DEG C of heating plates and heats 15 minutes, is activated；
Step 4, the drying of silica G plate: the silica G plate activated described in step 3 is put into drier, at one section
Between after by after drying silica G plate take out it is spare to get.
It is understood that needing to reflect using corresponding glycyrrhiza preparation after silica G plate is made according to above-mentioned steps
Not, to test the performance of silica G plate, steps are as follows for identification:
Step 1, the configuration of model drug test solution:
Take containing Radix Glycyrrhizae compound ox Siberian cocklebur particle (lot number: 20171212,20180208,20180327) powder 2.175g,
2.175g, 2.023g, respectively plus methanol 40ml is 30 minutes ultrasonic, and filtration, filtrate is evaporated, and residue adds water 40ml, uses n-butanol
20ml is extracted 3 times, merges n-butanol liquid, and water 20ml is added to wash 3 times, and n-butanol liquid is evaporated, and methanol 5ml is added to dissolve, as examination
Product solution；
Step 2, the configuration of Radix Glycyrrhizae control medicinal material solution:
Extracting liquorice control medicinal material (lot number: 120904-200410) 1.125g, add diethyl ether 40ml, ultrasound 30 minutes, filtration,
Filtrate is evaporated, and residue adds water 40ml, is extracted 3 times with n-butanol 20ml, merges n-butanol liquid, adds water 20ml to wash 3 times, n-butanol
Liquid is evaporated, and adds methanol 5ml to dissolve, as control medicinal material solution；Negative sample 1.125g separately is taken, it is molten with negative control is made for method
Liquid；
Step 3, thin-layer chromatographic analysis:
It is tested according to thin-layered chromatography (four general rules 0502 of Chinese Pharmacopoeia version in 2015), draws above-mentioned different lot numbers respectively
1 μ l of 2 μ l of test solution, 2 μ l of negative control solution and control medicinal material solution carries out point sample to above-mentioned processed silica G plate
And label, with ethyl acetate: formic acid: glacial acetic acid: solvent is made in water=15:1:1:2 ratio, by the silica G plate after point sample
It is placed in corresponding vessel and is unfolded, taken out after specified time, dried, spray with 10% ethanol solution of sulfuric acid, be heated to spot at 105 DEG C
Point colour developing is clear, sets and is inspected under ultraviolet lamp (365nm), it is as shown in Figure 9 to inspect result；
In Fig. 9: 1,2,3 is test sample, and it is that Radix Glycyrrhizae compares that lot number, which is respectively 20171212,20180208,20180327,4,
Medicinal material, 5 be the negative control for lacking Radix Glycyrrhizae；As shown in figure 9, under ultraviolet lamp, in sample chromatogram, with reference medicine chromatography
On corresponding position, there is corresponding principal spot to occur, and negative control is noiseless.
In conclusion in a kind of traditional Chinese medicine of licorice root preparation indentification by TLC of the invention silica G plate alkalization method: use
Silica G plate is impregnated 1 minute and is dried by 1%NaOH solution, and is activated 15 minutes in 105 DEG C.And prove through the foregoing embodiment, this
The alkalization method of silica G plate, can effectively improve Radix Glycyrrhizae thin-layer chromatography in a kind of traditional Chinese medicine of licorice root preparation indentification by TLC of invention
The chromatographic isolation degree of identification saves time cost, and qualification result is accurate, is easy to observe；Therefore, method proposed by the invention
Prefabricated alkaline silica gel G lamellae can be substituted, is applied in the Radix Glycyrrhizae indentification by TLC of Chinese materia medica preparation.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all in essence of the invention
Made any modifications, equivalent replacements, and improvements etc., should all be included in the protection scope of the present invention within mind and principle.
Claims (2)
1. the alkalization method of silica G plate in a kind of traditional Chinese medicine of licorice root preparation indentification by TLC, which is characterized in that including following step
It is rapid:
The configuration of alkalizing solution: step 1 takes a certain amount of sodium hydrate solid and the sodium hydroxide of distilled water configuration 1% molten
Liquid；
Step 2, impregnate silica G plate: silica G plate to be processed is put into fill described in step 1 1% sodium hydroxide it is molten
Dipping takes out silica G plate after 1 minute, 1 minute in the container of liquid；
Step 3, the activation of silica G plate: natural air drying after silica G plate described in step 2 is taken out, by the silica G after drying
Plate is placed in 105 DEG C of heating plates to heat 15-60 minutes and activate；The silica G plate that step activated is put into spare in drier.
2. the alkalization method of silica G plate, feature in a kind of traditional Chinese medicine of licorice root preparation indentification by TLC as described in claim 1
It is, the silica G plate is placed in 105 DEG C of heating plates in the step 3 and heats activation in 15 minutes.
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