CN109913955B - Preparation method of oriented growth polypeptide molecular array - Google Patents
Preparation method of oriented growth polypeptide molecular array Download PDFInfo
- Publication number
- CN109913955B CN109913955B CN201910234419.6A CN201910234419A CN109913955B CN 109913955 B CN109913955 B CN 109913955B CN 201910234419 A CN201910234419 A CN 201910234419A CN 109913955 B CN109913955 B CN 109913955B
- Authority
- CN
- China
- Prior art keywords
- polypeptide
- oriented growth
- array
- preparation
- graphene
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 229920001184 polypeptide Polymers 0.000 title claims abstract description 38
- 102000004196 processed proteins & peptides Human genes 0.000 title claims abstract description 38
- 108090000765 processed proteins & peptides Proteins 0.000 title claims abstract description 38
- 230000012010 growth Effects 0.000 title claims abstract description 19
- 238000002360 preparation method Methods 0.000 title abstract description 6
- 239000000758 substrate Substances 0.000 claims abstract description 16
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims abstract description 11
- 229910021389 graphene Inorganic materials 0.000 claims abstract description 11
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 claims abstract description 10
- 239000010949 copper Substances 0.000 claims abstract description 10
- 229910052802 copper Inorganic materials 0.000 claims abstract description 10
- 238000000034 method Methods 0.000 claims abstract description 10
- 238000004528 spin coating Methods 0.000 claims abstract description 6
- 238000001035 drying Methods 0.000 claims abstract description 5
- ZMXDDKWLCZADIW-UHFFFAOYSA-N N,N-Dimethylformamide Chemical compound CN(C)C=O ZMXDDKWLCZADIW-UHFFFAOYSA-N 0.000 claims description 12
- 239000003153 chemical reaction reagent Substances 0.000 claims description 6
- 150000004676 glycans Chemical class 0.000 claims description 4
- 239000011259 mixed solution Substances 0.000 claims description 4
- 229920001282 polysaccharide Polymers 0.000 claims description 4
- 239000005017 polysaccharide Substances 0.000 claims description 4
- 239000000243 solution Substances 0.000 claims description 4
- 239000002904 solvent Substances 0.000 claims description 4
- 238000001291 vacuum drying Methods 0.000 claims description 4
- 230000010287 polarization Effects 0.000 claims description 2
- 230000002269 spontaneous effect Effects 0.000 claims description 2
- 239000012620 biological material Substances 0.000 abstract description 6
- 230000000694 effects Effects 0.000 abstract description 2
- 239000003960 organic solvent Substances 0.000 abstract 1
- VORIUEAZEKLUSJ-UHFFFAOYSA-M [(6-chlorobenzotriazol-1-yl)oxy-(dimethylamino)methylidene]-dimethylazanium;trifluoroborane;fluoride Chemical compound [F-].FB(F)F.C1=C(Cl)C=C2N(OC(N(C)C)=[N+](C)C)N=NC2=C1 VORIUEAZEKLUSJ-UHFFFAOYSA-M 0.000 description 4
- 238000003491 array Methods 0.000 description 3
- 238000001878 scanning electron micrograph Methods 0.000 description 3
- 238000011160 research Methods 0.000 description 2
- 238000012546 transfer Methods 0.000 description 2
- 241000784732 Lycaena phlaeas Species 0.000 description 1
- 230000024245 cell differentiation Effects 0.000 description 1
- 230000010261 cell growth Effects 0.000 description 1
- 239000013078 crystal Substances 0.000 description 1
- 238000002425 crystallisation Methods 0.000 description 1
- 230000008025 crystallization Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000012377 drug delivery Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 230000001172 regenerating effect Effects 0.000 description 1
- 238000001338 self-assembly Methods 0.000 description 1
- 239000004065 semiconductor Substances 0.000 description 1
- 238000012163 sequencing technique Methods 0.000 description 1
- 230000008054 signal transmission Effects 0.000 description 1
- 238000002626 targeted therapy Methods 0.000 description 1
Images
Landscapes
- Crystals, And After-Treatments Of Crystals (AREA)
- Peptides Or Proteins (AREA)
- Carbon And Carbon Compounds (AREA)
Abstract
The invention discloses a preparation method of an oriented growth polypeptide molecular array. The method mainly comprises the following steps: dissolving a certain amount of polypeptide in an organic solvent, fully and uniformly oscillating to form a solution, dripping a proper amount of the solution on a graphene/copper substrate, carrying out spin coating by using a spin coater, and finally drying under a vacuum condition to obtain the oriented growth polypeptide. The method has the advantages of obvious effect, simple process and lower cost, and has profound significance for intrinsic application of the biological material and expansion of the biological material in other fields such as piezoelectric.
Description
Technical Field
The invention relates to a preparation method of an oriented growth polypeptide molecular array, belonging to the field of electrostatic force induced biological material growth.
Background
The polypeptide is a material which plays a significant role in the biomedical field and even the semiconductor field, and has particularly key roles in biosensing, cell growth and differentiation induction, drug delivery, targeted therapy, immune regenerative medicine, piezoelectric application and the like. Therefore, researchers have been keen on the self-assembly of biomaterials with fine and controllable growth, which is very important for the improvement and expansion of the functionality and application range of biomaterials. However, in the current research, the growth of polypeptide molecules is often controlled by an external field, a template method, a changed crystallization mode and the like, and the methods have the problems of low controllability, high cost and the like.
The research aims to obtain an oriented growth and arrangement polypeptide molecule array, charges are generated on the surface of graphene by utilizing charge transfer between the graphene and copper, and the electrostatic force generated by the charges of special one-dimensional arrangement on the surface is used for inducing one-dimensional arrangement of polar polypeptide molecules by combining the special structures of the graphene and the copper. It is expected that the polypeptide molecule array obtained by the method can generate vector signal transmission under certain conditions due to the orientation of the polypeptide molecule array, so that the polypeptide molecule array can induce growth sequencing of certain cell tissues and the like or be used as a targeted information site. In addition, recent researchers have focused on the piezoelectric properties exhibited by polypeptide arrays and expanded the electrical applications of such biomaterials. Therefore, the method for realizing ordered oriented growth arrangement of polypeptide molecules has profound significance and wide application prospect.
Disclosure of Invention
The invention provides a preparation method of an oriented growth polypeptide molecular array, which comprises the following steps:
1) dissolving the polypeptide reagent in N, N-dimethylformamide solvent, and fully oscillating and ultrasonically dispersing for at least 20min to form a uniform solution.
2) Dripping the mixed solution obtained in the step 1) on a graphene/copper substrate, and performing spin coating by using a spin coater.
3) And (3) placing the substrate after spin coating into a vacuum drying oven for natural drying to obtain the polypeptide array with oriented growth.
The substrate must be a graphene/copper substrate.
The polypeptide reagent is a polypeptide molecule with surface charge groups or spontaneous polarization in the interior. The polypeptide reagent may be TCTU (C)11H15ClN5OBF4) Low viscosity polysaccharide polypeptides, and the like.
The preparation process is simple, the process is very easy to control, and the obtained polypeptide array has the phenomenon of large-area orientation arrangement, and is different from the phenomenon that only small crystal particles are randomly arranged on other substrates (ITO glass substrates, Si substrates, copper substrates and the like) under the same condition. In addition, a large number of development experiments show that due to charge transfer between graphene and copper, the electrostatic effect of charges specially distributed on the surface of graphene enables polar polypeptide molecules to show an ordered arrangement phenomenon.
Drawings
Fig. 1 is an SEM image of an array of oriented growth TCTU polypeptide molecules prepared in example 1.
FIG. 2 is an SEM image of an oriented growth low viscosity polysaccharide polypeptide molecular array prepared in example 2.
Fig. 3 is an SEM image of an unoriented TCTU polypeptide molecule array prepared on a common copper substrate.
Detailed Description
The technical scheme of the invention is further illustrated by the following examples.
Example 1
1) 0.5g of polypeptide reagent TCTU is taken and dissolved in 10mL of N, N-dimethylformamide solvent, and the mixture is fully oscillated and ultrasonically dispersed for 20min to form uniform solution.
2) And (3) dropping a certain amount of mixed solution on the graphene/copper substrate, and performing spin coating by using a spin coater for 1000-to-3 seconds and 3000-to-30 seconds.
3) And (4) putting the spin-coated substrate into a vacuum drying oven for natural drying.
Example 2
1) Dissolving 0.5g of low-viscosity polysaccharide polypeptide molecule powder in 10mL of N, N-dimethylformamide solvent, and fully oscillating and ultrasonically dispersing for 20min to form a uniform solution.
2) And (3) dropping a certain amount of mixed solution on the graphene/copper substrate, and performing spin coating by using a spin coater for 1000-to-3 seconds and 3000-to-30 seconds.
3) And (4) putting the spin-coated substrate into a vacuum drying oven for natural drying.
SEM of the oriented growth polypeptide molecule arrays prepared in examples 1 and 2 is shown in FIG. 1 and FIG. 2, and it can be seen that the oriented growth polypeptide molecule arrays prepared according to the technical scheme of the present invention exhibit a phenomenon of one-dimensional ordered arrangement.
Claims (3)
1. A method for preparing an oriented growth polypeptide molecular array is characterized by comprising the following steps:
1) dissolving a polypeptide reagent in an N, N-dimethylformamide solvent, and fully oscillating and ultrasonically dispersing for at least 20min to form a uniform solution;
2) dripping the mixed solution obtained in the step 1) on a graphene/copper substrate, and spin-coating by using a spin coater;
3) putting the spin-coated substrate into a vacuum drying oven for natural drying;
the polypeptide reagent is low-viscosity polysaccharide polypeptide with surface charge groups or spontaneous polarization in the interior.
2. An array of oriented growth polypeptide molecules produced by the method of claim 1.
3. The anay of polypeptide molecules of claim 2, wherein said polypeptide molecules are in a one-dimensional ordered arrangement.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910234419.6A CN109913955B (en) | 2019-03-26 | 2019-03-26 | Preparation method of oriented growth polypeptide molecular array |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910234419.6A CN109913955B (en) | 2019-03-26 | 2019-03-26 | Preparation method of oriented growth polypeptide molecular array |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN109913955A CN109913955A (en) | 2019-06-21 |
| CN109913955B true CN109913955B (en) | 2020-11-03 |
Family
ID=66966783
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910234419.6A Active CN109913955B (en) | 2019-03-26 | 2019-03-26 | Preparation method of oriented growth polypeptide molecular array |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109913955B (en) |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR101603766B1 (en) * | 2009-11-13 | 2016-03-15 | 삼성전자주식회사 | Graphene laminate and process for preparing the same |
| CN102875639A (en) * | 2012-09-26 | 2013-01-16 | 深圳翰宇药业股份有限公司 | Solid-phase synthetic method of peptide and peptide synthesized by same |
| WO2014143954A2 (en) * | 2013-03-15 | 2014-09-18 | Arizona Board Of Regents On Behalf Of Arizona State University | Biosensor microarray compositions and methods |
| CN103526202B (en) * | 2013-09-30 | 2016-04-13 | 北京纳米能源与系统研究所 | A kind ofly prepare method of orientation polypeptide monomolecular film and products thereof |
| CN206217267U (en) * | 2016-10-28 | 2017-06-06 | 史广洲 | A kind of Graphene Copper Foil diaphragm |
| US10828869B2 (en) * | 2017-08-30 | 2020-11-10 | Ultra Conductive Copper Company, Inc. | Graphene-copper structure and manufacturing method |
-
2019
- 2019-03-26 CN CN201910234419.6A patent/CN109913955B/en active Active
Non-Patent Citations (1)
| Title |
|---|
| Growth of large-area aligned pentagonal graphene domains on high-index copper surfaces;XIA Kailun, et al.;《NANO RESEARCH》;20160731;第9卷(第7期);全文 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN109913955A (en) | 2019-06-21 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN107309146B (en) | Preparation method and application of micro-nano structure film | |
| KR102050199B1 (en) | Method for preparing graphene sphere electroconductive adhesive and graphene sphere electroconductive adhesive | |
| Xavier Mendes et al. | Enhanced electroactivity, mechanical properties, and printability through the addition of graphene oxide to photo-cross-linkable gelatin methacryloyl hydrogel | |
| JP2004124088A (en) | Block copolymer and method for manufacturing micro phase separation structure membrane | |
| US11724940B2 (en) | Method for forming graphene film through horizontally tiling and self-assembling graphene | |
| CN102826539A (en) | Hyperbranched polyaramide functionalized graphene, as well as preparation method and applications of hyperbranched polyaramide functionalized graphene | |
| US20120118498A1 (en) | Method and apparatus for forming a continuous oriented structure of a polymer | |
| CN106084264B (en) | Preparation process of composite material laminated plate with directionally arranged carbon nano tubes | |
| CN103408931B (en) | Preparation method of hybrid material compounded from fullerene micro-nano material and conjugated polymer | |
| Gao et al. | Morphology control of electrosprayed core–shell particles via collection media variation | |
| CN109913955B (en) | Preparation method of oriented growth polypeptide molecular array | |
| CN1293649C (en) | Preparation method of large surface area carbon nano pipe film for field emitting display cathode | |
| Lu et al. | Synthesis of submicron PEDOT particles of high electrical conductivity via continuous aerosol vapor polymerization | |
| CN105967690B (en) | A kind of method of superfine powder and large-size particles batch mixing | |
| CN109972136B (en) | Preparation method of oriented growth P (VDF-TrFE) film | |
| CN103396548A (en) | Preparation method of high-dielectric polyimide/calcium copper titanate nanowire composite material | |
| CN104448312A (en) | High-dielectric-constant polyimide film provided with multiwalled carbon nanotubes and preparation method of high-dielectric-constant polyimide film | |
| CN104020613B (en) | Alignment method and liquid crystal display panel | |
| CN103755958B (en) | A kind of preparation method of polyimides/CaCu 3 Ti 4 O coated with silver nano particle composite material | |
| CN106025101A (en) | Method for regulating and controlling growth of spherical rubrene crystal thin film by cosolvent through polymer induction layer | |
| CN110759338A (en) | Method for preparing large-size graphene based on laser fixed-point processing | |
| CN111665676B (en) | Construction method of liquid crystal limited domain space assembly microarray and liquid crystal composite material prepared by same | |
| CN110580986B (en) | Silver nanowire conductive film and preparation method thereof | |
| CN113621331A (en) | Method for preparing anisotropic conductive adhesive film by coating microspheres with nano carbon material and application of anisotropic conductive adhesive film | |
| CN106986960A (en) | It is a kind of to prepare graphene three-dimensional construction method of the powder body material containing carbon polymer |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |