CN109913526A - Microorganism is identifying and/or is distinguishing the application in not agnate individual - Google Patents
Microorganism is identifying and/or is distinguishing the application in not agnate individual Download PDFInfo
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- CN109913526A CN109913526A CN201910112205.1A CN201910112205A CN109913526A CN 109913526 A CN109913526 A CN 109913526A CN 201910112205 A CN201910112205 A CN 201910112205A CN 109913526 A CN109913526 A CN 109913526A
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Abstract
The present invention provides microorganisms to identify and/or distinguish the application in not agnate individual in the product for identifying and/or distinguishing the application and detection bacterial abundance in not agnate individual, the present invention also provides a kind of identification and/or the methods of the not agnate individual of differentiation, the method includes detecting the relative abundance of the Acinetobacter Pseudomonas in individual enteron aisle and/or excrement, and and threshold value comparison.
Description
Technical field
The present invention relates to biomedicine technical fields, and in particular to microorganism is identifying and/or distinguishing not agnate individual
In application, more particularly to detection Acinetobacter Pseudomonas relative abundance, and and threshold value comparison, identify individual species
The method in race source.
Background technique
Altitude sickness, that is, altitude sickness is after people reaches certain height above sea level, and body is caused by adapting to due to height above sea level
Draught head, oxygen content are few, are air-dried etc. variation and the natural physiological reaction generated.The symptom of altitude sickness normally behaves as
Headache, palpitation, tired, uncomfortable in chest, shortness of breath, vomiting, appetite stimulator, twitch, in a trance, the cognitive ability rapid drawdown of consciousness etc..Sign is the heart
Rate quickening, exaggerated respiration, blood pressure mile abnormality, face or edema of limbs, die Blausucht etc..Plateau heart disease is the one of altitude sickness
Kind, the cardiac structure function due to caused by the hypobaric hypoxia environment of plateau is impaired, is damaged with pulmonary hypertension and right heart function as spy
Sign.The Tibetan resident of Qinghai-Tibet Platean is global main High aititude crowd, has carried out long-term to hypoxemia, the hypobaric on plateau
Physiology and genetic adaptation, Anoxemia reduces under low-oxygen environment, has better locomitivity.
There are a large amount of symbiosis flora in human body intestinal canal, quantity is more than 1,000,000,000,000,000, about human body cell sum
10 times.The quantity of microbial gene is about 3,000,000 in enteron aisle simultaneously, about more than the 100 of human genome gene dosage
Times, the gene of such magnanimity can help microorganism to adapt to changeable environment, form the symbiosis inseparable with human body.
There are larger differences for its intestinal flora of the resident of different genetic backgrounds and living habit composition, such as Bacteroides is west resident
Relative amount is most in enteron aisle and the maximum bacterium category of inter-individual difference, South Korea resident are bacillus faecalis categories of dwelling.The difference of Pseudomonas
Imply individual to the tolerance of local environment.
For the diagnosis marker of tolerance of the individual under altitude environment or altitude sickness, research is extensive at present.Example
Such as, patent CN106248947A discloses the compound lipopolysaccharides of a kind of lipid and polysaccharide as mammal plateau brain edema mark
The purposes of will object, experiment detects the content into lipopolysaccharides in mammalian plasma after environment of low oxygen plateau, when rouge is more in blood plasma
When the content of sugar is more than 0.5EU/mL, mammalian brain water content is obviously increased.Patent CN104614462B discloses C8-
Ceramide, sphingol and glutamine detect the diagnostic reagent of this diagnosis marker as plateau pneumochysis diagnosis marker
The accuracy of box diagnostic result is high, provides quantifiable, objective sensitive clinical diagnosis index for the diagnosis of plateau pneumochysis.Specially
Sharp CN102605089B discloses the neurological susceptibility excessively increased using single nucleotide polymorphism as the high pronormoblast of marker detection,
In provide detection single nucleotide polymorphism primer and kit.Patent CN101135666B, CN101470095B,
CN101470096A, CN101135665B, CN101469345B, CN101135667B, CN1898394B are disclosed with monokaryon
Nucleotide polymorphism is the neurological susceptibility of marker detection plateau pneumochysis, and which provide detection single nucleotide polymorphism primer and examinations
Agent box.But so far, not of the same race to identify and/or distinguish there is not yet using Acinetobacter Pseudomonas as marker
The technical solution of race's individual.
Summary of the invention
The first aspect of the present invention provides microorganism and is identifying and/or distinguishing the application in not agnate individual.
Preferably, the microorganism is Acinetobacter Pseudomonas.
It is further preferred that the microorganism is the Acinetobacter Pseudomonas in enteron aisle or excrement.
In the specific embodiment of the present invention, the microorganism is in enteron aisle or excrement
Acinetobacter histaminiformans。
Preferably, the not agnate race and the race of Plain environment for altitude environment.
In the specific embodiment of the present invention, described is not agnate for Tibetan and Han nationality.
In the specific embodiment of the present invention, the not agnate Tibetan and the Chinese to live under altitude environment
Race.
The second aspect of the present invention provides a kind of method identified and/or distinguish not agnate individual, the method
The abundance of microorganism in enteron aisle and/or excrement including detecting the not agnate individual, by the Abundances of the microorganism
With threshold value comparison, determine that the not agnate individual is the race of altitude environment or the race of Plain environment.
Altitude environment of the present invention is height above sea level 2000m or more, has low pressure, the condition of anoxic.
Preferably, the altitude environment is height above sea level 2700m or more, under conditions of low pressure, anoxic.
In the specific embodiment of the present invention, the altitude environment be height above sea level 3500m or more, have low pressure,
Under conditions of anoxic.
In the specific embodiment of the present invention, the altitude environment be height above sea level 5500m or more, have low pressure,
Under conditions of anoxic.
Preferably, the method includes the following steps:
1) microbial nucleic acids in test individual excrement or enteron aisle are extracted；
2) amplification step 1) extract microbial nucleic acids；
3) nucleic acid of step 2) amplification is sequenced；
4) relative abundance of Acinetobacter Pseudomonas is calculated；
5) by the relative abundance value and 0.91 × 10 of Acinetobacter Pseudomonas described in step 4)-4It compares；
Wherein, the relative abundance value of Acinetobacter Pseudomonas is less than 0.91 × 10-4It is determined as people from Tibetan,
The relative abundance value of Acinetobacter Pseudomonas is greater than 0.91 × 10-4It is determined as the Hans.
The third aspect of the present invention provides the product of detection bacterial abundance in identification and/or distinguishes not agnate a
Application in body.
Preferably, the microorganism is Acinetobacter Pseudomonas.
Preferably, the not agnate race and the race of Plain environment for altitude environment.
The fourth aspect of the present invention, provides a kind of product for detecting bacterial abundance, and the product includes that detection is micro-
The reagent and/or device of biological relative abundance, it is preferred that the microorganism is Acinetobacter Pseudomonas.
The fifth aspect of the present invention, the reagent for providing detection Acinetobacter Pseudomonas assess individual plateau in preparation
Application under environment in the product of tolerance.
Preferably, the Acinetobacter Pseudomonas is the Acinetobacter Pseudomonas in enteron aisle.
The reagent of detection Acinetobacter Pseudomonas of the present invention is detection Acinetobacter Pseudomonas abundance
Reagent.
Preferably, the reagent of the detection Acinetobacter Pseudomonas is to detect in individual enteron aisle
The reagent of Acinetobacter Pseudomonas abundance.
In the specific embodiment of the present invention, the reagent of the detection Acinetobacter Pseudomonas is detection
The reagent of Acinetobacter Pseudomonas abundance in individual excrement.
Tolerance is the susceptible degree of altitude sickness under altitude environment of the present invention.
Altitude environment of the present invention is height above sea level 2000m or more, has low pressure, the condition of anoxic.
Preferably, the altitude environment is height above sea level 2700m or more, under conditions of low pressure, anoxic.
In the specific embodiment of the present invention, the altitude environment be height above sea level 3500m or more, have low pressure,
Under conditions of anoxic.
In the specific embodiment of the present invention, the altitude environment be height above sea level 5500m or more, have low pressure,
Under conditions of anoxic.
The sixth aspect of the present invention provides a kind of microorganism identified and/or distinguish not agnate individual, and described is micro-
Biology is Acinetobacter Pseudomonas.
Preferably, the microorganism is the Acinetobacter Pseudomonas in enteron aisle or excrement.
In the specific embodiment of the present invention, the microorganism is the Acinetobacter Pseudomonas in excrement.
The seventh aspect of the present invention provides a kind of marker for assessing altitude environment tolerance, the marker
For Acinetobacter Pseudomonas.
Preferably, the marker is the Acinetobacter Pseudomonas in enteron aisle.
In the specific embodiment of the present invention, the marker is the Acinetobacter Pseudomonas in excrement.
The eighth aspect of the present invention provides a kind of product for assessing tolerance under individual altitude environment, the production
Product include the reagent of detection Acinetobacter Pseudomonas.
Preferably, the reagent of the detection Acinetobacter Pseudomonas is to detect in individual enteron aisle
The reagent of Acinetobacter Pseudomonas abundance.
In the specific embodiment of the present invention, the reagent of the detection Acinetobacter Pseudomonas is detection
The reagent of Acinetobacter Pseudomonas abundance in individual excrement.
Product of the present invention includes the reagent of detection Acinetobacter Pseudomonas and the reagent for detecting other bacterium.
The ninth aspect of the present invention provides a kind of method for detecting Acinetobacter Pseudomonas abundance, the method
Including carrying out 16S rRNA sequencing, and reads progress OTU cluster and species annotation to sequencing acquisition to sample.
It preferably, further include being carried out to the reads that sequencing obtains after 16S rRNA sequencing, between OTU cluster
Low quality Partial Shear, the data for splitting each sample and/or removal chimera sequence.
Preferably, the clustering method be selected from sortmerna, mothur, trie, uclust_ref, usearch,
usearch_ref、blast、usearch61、usearch61_ref、sumaclust、swarm、prefix_suffix、cdhit
Or uclust.
Preferably, the clustering algorithm is selected from furthest, nearest or average.
Preferably, the annotation algorithm is selected from rdp, blast, rtax, mothur, uclust or sortmerna.
In the specific embodiment of the present invention, the described OTU cluster is with 97% consistency by Sequence clustering.
The tenth aspect of the present invention provides a kind of method for improving individual altitude environment tolerance, the method
Including transplanting Acinetobacter Pseudomonas to individual.
Preferably, the method includes the transplanting Acinetobacter Pseudomonas into individual enteron aisle.
In the specific embodiment of the present invention, the method is included in individual and goes before altitude environment to transplant
Acinetobacter Pseudomonas.
Preferably, the individual altitude environment tolerance of the raising is the energy for improving people from plains region and adapting to altitude environment
Power.
Altitude sickness of the present invention is selected from the acute high altitude sickness generated under altitude environment and/or chronic plateau sickness.Institute
The acute high altitude sickness stated is selected from altitude coma, plateau brain edema, plateau pneumochysis or brain, the simultaneous mixing of lung abnormal symptom
Type disease；And/or the chronic plateau sickness is low selected from plateau heart disease, altitude erythrocytosis, high altitude hypertension, plateau
The simultaneous mixed type disease of blood pressure, plateau heart disease, polycythemia.Preferably, the altitude sickness is anti-plateau
Cardiopulmonary and injury of blood vessel；It is further preferred that the altitude sickness is plateau heart disease.
In the specific embodiment of the present invention, the altitude sickness is pulmonary hypertension and/or right ventricular hypertrophy.
The clinical manifestation of altitude sickness of the present invention be selected from headache, giddy, palpitation, increased heart rate, tired, uncomfortable in chest, shortness of breath,
Exaggerated respiration, Nausea and vomiting, insomnia, out of strength, dim eyesight, drowsiness, appetite stimulator, twitch, in a trance, the numb in every limb, lip of consciousness refer to hair
The combination of one or more of dark purple, face oedema, edema of limbs or cognitive ability rapid drawdown.
The abundance or relative abundance of Acinetobacter Pseudomonas of the present invention are Acinetobacter Pseudomonas in spy
Determine the relative abundance in flora, the abundance journey for being in an embodiment of the present invention Acinetobacter Pseudomonas in intestinal flora
Degree.
Acinetobacter Pseudomonas of the present invention is to isolate and purify or buy from individual to obtain.
Acinetobacter Pseudomonas of the present invention is provided in form living.
Acinetobacter Pseudomonas of the present invention is provided in the form for being lyophilized, being freeze-dried or be spray-dried.
Acinetobacter Pseudomonas of the present invention is provided with spore form.
Acinetobacter Pseudomonas of the present invention is Acinetobacter histaminiformans.
"and/or" of the present invention includes selecting the project and any amount of projects combo that one lists.
" comprising " of the present invention is open description, containing described specified ingredient or step, and will not
Other the specified ingredients or step substantially influenced.
" identification " of the present invention indicate by one or more individual judge or be determined as one or two with
On attribute, for example, " identification " in the embodiment of the present invention is to judge or be determined as source for one or more individual
It is Han nationality or Tibetan.
More than two individuals are split up into different attributes by " differentiation " of the present invention expression, for example, the present invention is real
Applying " differentiation " described in example, source is Han nationality or source is Tibetan to divide into more than two individuals.
Detailed description of the invention
Hereinafter, carrying out the embodiment that the present invention will be described in detail in conjunction with attached drawing, in which:
Fig. 1: the abundance of Acinetobacter Pseudomonas in excrement is organized for Tibetan's soldier's group (Zang) and Han nationality soldier (Han)
Difference.
Fig. 2: Acinetobacter Pseudomonas relative abundance identifies and/or distinguishes the sensitivity that individual source is Han nationality or Tibetan
Property and specificity ROC curve.
Fig. 3: it is formed for group in Tibetan soldier's group (Zang) excrement.
Fig. 4: Han nationality soldier (Han) organizes group's composition in excrement.
Specific embodiment
Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, complete
Site preparation description, it is clear that described embodiment is only section Example of the invention, rather than all.Based in the present invention
Embodiment, every other embodiment obtained by those of ordinary skill in the art without making creative efforts, all
Belong to the scope of protection of the invention.
Embodiment 1
One, materials and methods:
1.1 study population
Design case-control study is stated according to STROBE, is included in certain moon in year to certain moon in year and is on active service in certain Chinese army
Tibetan male soldiers more than 128 acclimatization height above sea level, 3500 meters of plateaus is experimental group, flat according to Tibetan's soldier's age-matched 128
Former Han nationality soldier compares.The identical Chinese army mixed diet of the carry out of two groups of crowds of strict control, keeps identical sea
3500 meters of training environments and training strength are pulled out, smoking cessation, wine continue 3 months.Exclusion has chronic inflammatory disease, oral antibiotic, urgency
Sexuality dye and enterogastric diseases taxi soldier.
1.2 fecal specimens are collected
Each research object receives a closestool excrement collector after being included in, big after an action of the bowels by stool loading in discharge
Collecting dung pipe is stored in -20 DEG C of refrigerators at once, and stool sample is transported to -80 DEG C of refrigerators and saved by next day, and unified storage is standby
With.
1.3DNA extracting
Draw 1000 μ L cetyl trimethylammonium bromides (Hexadecyl trimethyl ammonium Bromide,
CTAB) in lysate to 2.0mL EP pipe, lysozyme is added, then the sample of 500 μ L or so is added in lysate, 65 DEG C of water
Bath, is during which mixed by inversion for several times, so that sample sufficiently cracks.Centrifuging and taking supernatant adds phenol (pH8.0): chloroform: isoamyl alcohol (25:
24:1), it is mixed by inversion, 12000rpm is centrifuged 10min.Supernatant is taken, chlorination is imitative: isoamyl alcohol (24:1) is mixed by inversion, 12000rpm
It is centrifuged 10min.It draws in supernatant to 1.5mL centrifuge tube, isopropanol is added, rocks up and down, -20 DEG C of precipitatings.12000rpm centrifugation
10 minutes, liquid is poured out, is careful not to pour out precipitating.With 75% ethanol washing of 1mL 2 times, remaining a small amount of liquid can again from
The heart is collected, and is then sucked out with pipette tips.Superclean bench drying or room temperature are dried.DdH is added2O dissolving DNA sample, adds
1 μ L of RNaseA digests RNA, 37 DEG C of placement 15min.Later using the purity and concentration of agarose gel electrophoresis detection DNA, take
Suitable sample DNA uses sterile water diluted sample DNA to 1ng/ μ L in centrifuge tube.
1.4PCR amplification, sample mixing and purifying
Using the genomic DNA after 1.3 dilutions as template, according to sequencing region 16S V3-V4, selection uses band Barcode
Special primer 515F and 806R, New England Biolabs companyHigh-Fidelity PCR
Master Mix with GC Buffer, and efficient high fidelity enzyme (Taq DNA Polymerase) it carries out
PCR, it is ensured that amplification efficiency and accuracy；
Wherein,
Specific primer 515F are as follows: 5 '-GTGCCAGCMGCCGCGGTAA-3 ' (SEQ ID NO:1)
Specific primer 806R are as follows: 5 '-GGACTACHVGGGTWTCTAAT-3 ' (SEQ ID NO:2).
PCR product carries out electrophoresis detection using the Ago-Gel of 2% concentration；It is mixed that equivalent is carried out according to PCR product concentration
Sample uses the agarose gel electrophoresis purified pcr product of 1 × TAE concentration 2%, shearing recycling target stripe after mixing well.Product
Purification kit uses Thermo Scientific company GeneJET plastic recovery kit to recycle PCR product.
1.5 library constructions and the sequencing of upper machine
Library kit is built using the Ion Plus Fragment Library Kit 48rxns of Thermofisher company
The building in library is carried out, the library built uses Thermofisher's after Qubit is quantitative and library detection is qualified
Ion S5TMXL carries out machine sequencing.
The analysis of 1.6 data
1.6.1 sequencing data is handled
It is first right using Cutadapt (V1.9.1, http://cutadapt.readthedocs.io/en/stable/)
Reads carries out low quality Partial Shear, splits out each sample data from obtained reads further according to Barcode, clips
Barcode and the preliminary Quality Control of primer sequence obtain initial data, and the Reads obtained after handling above needs to be removed embedding
The processing of fit sequence, Reads sequence are finally removed by the way that detection chimera sequence is compared with species annotations database
Chimera sequence therein, obtains final valid data.
1.6.2 taxonomical unit (operational taxonomic unit, the OUT) cluster and species annotation of operation
Using Uparse software (Uparse v7.0.1001, http://www.drive5.com/uparse/) to all
Whole valid data of sample are clustered, and Sequence clustering is become OTUs (Operational with 97% consistency by default
Taxonomic Units), while the representative series of OTUs can be chosen, according to its algorithm principle, screening is that occur in OTUs
Representative sequence of the highest sequence of frequency as OTUs.Species annotation is carried out to OTUs sequence, with Mothur method and SILVA
The SSUrRNA database of (http://www.arb-silva.de/) carries out the analysis of species annotation (given threshold is 0.8~1),
It obtains taxonomic information and forms (see Fig. 3,4) in the group for belonging to level statistic Han nationality soldier's group, Tibetan soldier group respectively.
1.6.3 the calculating of Pseudomonas relative abundance and comparison in difference
All Pseudomonas OTU value tables that Pseudomonas relative abundance is arrived with Acinetobacter Pseudomonas OTU value/detection pattern detection
Show, comparison in difference is because sample Non-Gaussian Distribution is examined using Mann-Whitney.Draw ROC curve identify/distinguish individual species
The sensibility and specificity that race source or assessment Pseudomonas relative abundance diagnose tolerance under altitude environment, calculates dividing value, P <
0.05 is statistically significant for difference, carries out statistical procedures using SPSS22.0 software package.
Two, experimental result
Acinetobacter Pseudomonas relative abundance difference in 2.1 Han nationality and Tibetan populations stool sample
The relative abundance [median (quartile)] of Acinetobacter Pseudomonas is [2.32 × 10 in Chinese Han Population-4
(0.93×10-4, 4.70 × 10-4)], the relative abundance [median (quartile of Acinetobacter Pseudomonas in Tibetan populations
Number)] it is 0.01 × 10-4(0,0.09 × 10-4), show that Tibetan populations Acinetobacter Pseudomonas relative abundance is substantially less than the Chinese
Clansman group, difference have conspicuousness, as shown in Figure 1.
2.2 Acinetobacter Pseudomonas relative abundances identify/distinguish the sensibility and specificity in the source Han Zang
As shown in Fig. 2, ROC curve shows that Acinetobacter Pseudomonas relative abundance identifies/distinguish the source Han Zang or assessment
The sensibility and specificity of tolerance under altitude environment, area (Area Under Curve, AUC) value under ROC curve are
0.965, Pseudomonas relative abundance is 0.91 × 10-4When, accuracy (Accuracy) is 0.941, and specificity 0.968 is sensitive
It is 0.944 that degree, which is 0.911, F1-Score value, and maximum youden index 0.64 can be obtained.
The above results, which show to be sequenced using 16SrRNA, detects stool sample Acinetobacter Pseudomonas relative abundance, can
It is Tibetan or Han nationality to identify crowd source, alternatively, assessment is individual for tolerance under altitude environment, when relative abundance is big
In 0.91 × 10-4It can determine that individual is Chinese group people, and very strong for tolerance under altitude environment.
2 confirmatory experiment of embodiment
Design case-control study is stated according to STROBE, selects 5 volunteers, and 5 volunteers of strict control carry out phase
Same Chinese army's mixed diet, keeps 3500 meters of environment of identical height above sea level and exercise intensity, and smoking cessation, wine continue 3 months.5
Volunteer excludes have chronic inflammatory disease, oral antibiotic, acute infection and enterogastric diseases.
Experimental procedure:
1, fecal specimens are collected；
2, DNA is extracted；
3, PCR amplification, sample mixing and purifying；
4, library construction and the sequencing of upper machine；
5, sequencing data is handled；
6, the taxonomical unit cluster and species annotation of operation.Step 1-6 concrete operations are as described in Example 1.
Experimental result
In 5 volunteers, there are 3 volunteer's Acinetobacter Pseudomonas relative abundance values lower than threshold value, be judged to hiding
Race；2 volunteer's Acinetobacter Pseudomonas relative abundance values are higher than threshold value, are determined as Han nationality.Experiment detection after through inquiry and
Investigation confirms that 3 volunteers are Tibetan really, and 2 volunteers are Han nationality really, and experimental result is accurate.
The preferred embodiment of the present invention has been described above in detail, still, during present invention is not limited to the embodiments described above
Detail within the scope of the technical concept of the present invention can be with various simple variants of the technical solution of the present invention are made, this
A little simple variants all belong to the scope of protection of the present invention.
It is further to note that specific technical features described in the above specific embodiments, in not lance
In the case where shield, can be combined in any appropriate way, in order to avoid unnecessary repetition, the present invention to it is various can
No further explanation will be given for the combination of energy.
Sequence table
<110>Chinese People's Liberation Army General Hospital
<120>microorganism is identifying and/or is distinguishing the application in not agnate individual
<130> 1
<160> 2
<170> PatentIn version 3.5
<210> 1
<211> 19
<212> DNA/RNA
<213>artificial sequence (Artificial Sequence)
<400> 1
gtgccagcmg ccgcggtaa 19
<210> 2
<211> 20
<212> DNA/RNA
<213>artificial sequence (Artificial Sequence)
<400> 2
ggactachvg ggtwtctaat 20
Claims (10)
1. microorganism is identifying and/or is distinguishing the application in not agnate individual, which is characterized in that the microorganism is
Acinetobacter Pseudomonas.
2. application according to claim 1, which is characterized in that the microorganism is in enteron aisle or excrement
Acinetobacter Pseudomonas.
3. application according to claim 1 or 2, which is characterized in that the microorganism is in enteron aisle or excrement
Acinetobacter histaminiformans。
4. application according to claim 1 to 3, which is characterized in that the not agnate race for altitude environment
With the race of Plain environment.
5. application according to claim 1 to 4, which is characterized in that described is not agnate for Tibetan and Han nationality.
6. a kind of method of identification and/or the not agnate individual of differentiation, which is characterized in that the method includes that detection is described not
The abundance of microorganism in the enteron aisle and/or excrement of agnate individual determines the Abundances and threshold value comparison of the microorganism
The not agnate individual is the race of altitude environment or the race of Plain environment.
7. according to the method described in claim 6, it is characterized in that, the method includes the following steps:
1) microbial nucleic acids in test individual excrement or enteron aisle are extracted；
2) amplification step 1) extract microbial nucleic acids；
3) nucleic acid of step 2) amplification is sequenced；
4) relative abundance of Acinetobacter Pseudomonas is calculated；
5) by the relative abundance value and 0.91 × 10 of Acinetobacter Pseudomonas described in step 4)-4It compares；
Wherein, the relative abundance value of Acinetobacter Pseudomonas is less than 0.91 × 10-4It is determined as people from Tibetan, Acinetobacter
The relative abundance value of Pseudomonas is greater than 0.91 × 10-4It is determined as the Hans.
8. the product for detecting bacterial abundance is identifying and/or is distinguishing the application in not agnate individual.
9. application according to claim 8, which is characterized in that the microorganism is Acinetobacter Pseudomonas；Or
Person, the not agnate race and the race of Plain environment for altitude environment.
10. a kind of product for detecting bacterial abundance, which is characterized in that the product includes detection microorganism relative abundance
Reagent and/or device, it is preferred that the microorganism is Acinetobacter Pseudomonas.
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| CN201910112205.1A CN109913526B (en) | 2019-02-13 | 2019-02-13 | Use of microorganisms for identifying and/or differentiating different ethnic groups of individuals |
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| CN201910112205.1A CN109913526B (en) | 2019-02-13 | 2019-02-13 | Use of microorganisms for identifying and/or differentiating different ethnic groups of individuals |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114566224A (en) * | 2022-03-09 | 2022-05-31 | 中国人民解放军总医院 | Model for identifying or distinguishing different altitude crowds and application thereof |
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| EP1083234A1 (en) * | 1998-05-27 | 2001-03-14 | Mitsubishi Chemical Corporation | Process for producing l-ribose |
| CN101248188A (en) * | 2005-06-28 | 2008-08-20 | 株式会社东芝 | Method, array, apparatus and test system to discriminate individuals |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN114566224A (en) * | 2022-03-09 | 2022-05-31 | 中国人民解放军总医院 | Model for identifying or distinguishing different altitude crowds and application thereof |
| CN114566224B (en) * | 2022-03-09 | 2023-08-11 | 中国人民解放军总医院 | Model for identifying or distinguishing people at different altitudes and application thereof |
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| CN109913526B (en) | 2021-03-26 |
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