CN109907002A - The method of breeding fine breed cow is independently matched based on embryo biotechnology - Google Patents
The method of breeding fine breed cow is independently matched based on embryo biotechnology Download PDFInfo
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- CN109907002A CN109907002A CN201910236032.4A CN201910236032A CN109907002A CN 109907002 A CN109907002 A CN 109907002A CN 201910236032 A CN201910236032 A CN 201910236032A CN 109907002 A CN109907002 A CN 109907002A
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Abstract
The invention discloses the methods that breeding fine breed cow is independently matched based on embryo biotechnology, including introduced variety embryo and frozen semen, test and analyze number, software and matching, analysis selection number, determine corresponding kind with embryo and frozen semen, embryo technology operation, receptor replace-conceive and sex identification step；The method that breeding fine breed cow is independently matched based on embryo biotechnology in the present invention, it introduces international top kind can provide good gene source with frozen semen with embryo and international top kind for fine breed cow, and it is based on embryo biotechnology, the operation such as gene group selection or monosperm injection intracellular is carried out with embryo and frozen semen to kind, it is strong to cultivate independence, the milk cow breeding generation inteval can substantially be shortened, improve kind of an obstinacy energy, it reduces and cultivates cost, optimize the Industry Model of enterprise, improves enterprise's profitability and the ability to ward off risks.
Description
Technical field
The present invention relates to embryo biotechnology fields, breed breeding more particularly to independently matching based on embryo biotechnology
The method of milk cow.
Background technique
In order to accelerate the breeding speed of milk cow and improve the population quality of milk cow, China is raw to embryo since the seventies
Object engineering technology is studied, and research and development speed is accelerated after the eighties, in recent years, in embryo's bioengineering single technology side
Face achieves gratifying research achievement.Especially estrus synchronization, superfecundation, in vitro fertilization, embryo's mobile phone, sex identification,
In terms of the individual events embryo such as embryo separating, embryo cloning, Embryo freezing preservation and embryo transfer biotechnology, have larger prominent
Broken and progress, accelerates the breeding of milk cow and the improvement of quality to a certain extent.
Nonetheless, the mode that existing dairy cultivates milk cow still has significant limitation, such as there are milk cows
The kind situation of low quality with embryo or kind frozen semen, is difficult to promote the total quality of kind ox, and by the limit of region
System and influence, the gene of fine breed cow is difficult to obtain, and can not carry out autonomous apolegamy, materially increases cultivation
Fine breed cow cost.
Therefore, in view of the above technical problems, it is necessary to provide and breeding fine breed cow is independently matched based on embryo biotechnology
Method.
Summary of the invention
In view of this, the purpose of the present invention is to provide the sides for independently matching breeding fine breed cow based on embryo biotechnology
Method can substantially shorten milk cow and breed the generation inteval, improve kind of an obstinacy energy, reduce and cultivate cost, and cultivation independence is strong, by ground
Domain influences small.
To achieve the goals above, the technical solution that one embodiment of the invention provides is as follows: certainly based on embryo biotechnology
The method of main apolegamy breeding fine breed cow, comprising the following steps:
S1: according to the relevant law of country and regulation, international top kind of introduction smart with freezing with international top kind with embryo
Liquid；
S2: the number that the kind in S1 is distinguished with embryo and frozen semen, number has uniqueness, and carries out one by one
Genetic test and analysis, reference numeral records all analysis data, and corresponding label makes mutual corresponding electronics mark respectively
Label；
S3: the analysis data in S2 are uploaded into database, corresponding pre-matching software are made, by the kind embryo in database
Tire and frozen semen data carry out pre- pair analysis, and generate analysis comparison report one by one；
S4: according to the pre- pairing in S3 as a result, choose one or more groups of kinds to be numbered with embryo and frozen semen, and foundation
Corresponding number searches corresponding kind with embryo and frozen semen；
S5: according to the genetic analysis data in S2, genome is carried out with embryo and frozen semen to the kind of the selection in S4
The operation such as selection or monosperm injection intracellular；
S6: being operated to combine kind with embryo and frozen semen by corresponding technical staff and form fertilized eggs, is placed into advance
The receptor cow intrauterine of processing；
S7: the intrauterine embryo of receptor cow carries out sex identification, is decided whether to terminate receptor cow according to appraisal
Gestation carry out the operation of terminal pregnancy when needing terminal pregnancy, and repeat S6, when being not necessarily to terminal pregnancy, cultivate to by
The childbirth of body cow.
As a further improvement of the present invention, in S1, top kind of the world is mainly given birth to the donor bull of frozen semen
Produce performance estimation breeding value or select index it is top ranked be 10% before exporting country within, and Genetic Evaluation high reliablity in
70%.
As a further improvement of the present invention, in S1, donor cows main productivity of top kind of the world with embryo
Top ranked energy estimated breeding value is within before exporting country 15%.
As a further improvement of the present invention, in S2, the electronic tag is RFID label tag.
As a further improvement of the present invention, in S2, the number includes letter and number.
As a further improvement of the present invention, described kind is W with the number initial of embryo, the lead-in of the frozen semen
Mother is M.
As a further improvement of the present invention, in S3, the comparison report content includes gene information and combination advantage letter
Breath.
As a further improvement of the present invention, after the receptor cow childbirth target milk cow, the gene of target milk cow is recorded
And performance shape, while uploading database.
The beneficial effects of the present invention are: the side for independently matching breeding fine breed cow based on embryo biotechnology in the present invention
Method, introduce international top kind with embryo and it is international top kind with frozen semen can be provided for fine breed cow good gene come
Source, and it is based on embryo biotechnology, the behaviour such as gene group selection or monosperm injection intracellular is carried out with embryo and frozen semen to kind
Make, cultivation independence is strong, can substantially shorten the milk cow breeding generation inteval, improve kind of an obstinacy energy, reduces cultivation cost, optimizes enterprise
Industry Model, improve enterprise's profitability and the ability to ward off risks.
Detailed description of the invention
In order to more clearly explain the embodiment of the invention or the technical proposal in the existing technology, to embodiment or will show below
There is attached drawing needed in technical description to be briefly described, it should be apparent that, the accompanying drawings in the following description is only this
The some embodiments recorded in invention, for those of ordinary skill in the art, without creative efforts,
It is also possible to obtain other drawings based on these drawings.
Fig. 1 is flow diagram in a specific embodiment of the invention.
Specific embodiment
Technical solution in order to enable those skilled in the art to better understand the present invention, below in conjunction with of the invention real
The attached drawing in example is applied, technical scheme in the embodiment of the invention is clearly and completely described, it is clear that described implementation
Example is only a part of the embodiment of the present invention, instead of all the embodiments.Based on the embodiments of the present invention, this field is common
Technical staff's every other embodiment obtained without making creative work, all should belong to protection of the present invention
Range.
Join shown in Fig. 1, in a specific embodiment of the invention, breeding fine breed cow is independently matched based on embryo biotechnology
Method, comprising the following steps:
S1: according to the relevant law of country and regulation, international top kind of introduction smart with freezing with international top kind with embryo
Liquid；
S2: the number that the kind in S1 is distinguished with embryo and frozen semen, number has uniqueness, and carries out one by one
Genetic test and analysis, reference numeral records all analysis data, and corresponding label makes mutual corresponding electronics mark respectively
Label；
S3: the analysis data in S2 are uploaded into database, corresponding pre-matching software are made, by the kind embryo in database
Tire and frozen semen data carry out pre- pair analysis, and generate analysis comparison report one by one；
S4: according to the pre- pairing in S3 as a result, choose one or more groups of kinds to be numbered with embryo and frozen semen, and foundation
Corresponding number searches corresponding kind with embryo and frozen semen；
S5: according to the genetic analysis data in S2, genome is carried out with embryo and frozen semen to the kind of the selection in S4
The operation such as selection or monosperm injection intracellular；
S6: being operated to combine kind with embryo and frozen semen by corresponding technical staff and form fertilized eggs, is placed into advance
The receptor cow intrauterine of processing；
S7: the intrauterine embryo of receptor cow carries out sex identification, is decided whether to terminate receptor cow according to appraisal
Gestation carry out the operation of terminal pregnancy when needing terminal pregnancy, and repeat S6, when being not necessarily to terminal pregnancy, cultivate to by
The childbirth of body cow.
Further, in S1, international top kind with the donor bull Main Production Rerfomance estimated breeding value of frozen semen or
Select index it is top ranked be 10% before exporting country within, and Genetic Evaluation high reliablity is in 70%, international top kind with
The top ranked donor cows Main Production Rerfomance estimated breeding value of embryo is within before exporting country 15%.
Further, in S2, electronic tag is RFID label tag, and number includes letter and number, and kind is first with the number of embryo
Letter is W, and the initial of frozen semen is M.
Further, in S3, comparison report content includes gene information and combination advantage information.
Further, after receptor cow childbirth target milk cow, the gene and performance shape of target milk cow is recorded, is uploaded simultaneously
Database is verified convenient for the accuracy to pre-matching information, and and the standard with match information is substantially improved by big data
True property.
As can be seen from the above technical solutions, the invention has the following advantages: it is biological based on embryo in the present invention
The method that technology independently matches breeding fine breed cow, introducing international top kind can with frozen semen with embryo and top kind of the world
Good gene source is provided for fine breed cow, and is based on embryo biotechnology, carries out gene with embryo and frozen semen to kind
The operation such as group selection or monosperm injection intracellular, cultivation independence is strong, can substantially shorten the milk cow breeding generation inteval, improve kind of an ox
Performance reduces and cultivates cost, optimizes the Industry Model of enterprise, improves enterprise's profitability and the ability to ward off risks.
The method that breeding fine breed cow is independently matched based on embryo biotechnology in the present invention, can substantially shorten milk cow
The breeding generation inteval improves kind of an obstinacy energy, reduces and cultivates cost, and cultivation independence is strong, small by regional impact.
It is obvious to a person skilled in the art that invention is not limited to the details of the above exemplary embodiments, Er Qie
In the case where without departing substantially from spirit or essential attributes of the invention, the present invention can be realized in other specific forms.Therefore, no matter
From the point of view of which point, the present embodiments are to be considered as illustrative and not restrictive, and the scope of the present invention is by appended power
Benefit requires rather than above description limits, it is intended that all by what is fallen within the meaning and scope of the equivalent elements of the claims
Variation is included within the present invention.Any reference signs in the claims should not be construed as limiting the involved claims.
In addition, it should be understood that although this specification is described in terms of embodiments, but not each embodiment is only wrapped
Containing an independent technical solution, this description of the specification is merely for the sake of clarity, and those skilled in the art should
It considers the specification as a whole, the technical solutions in the various embodiments may also be suitably combined, forms those skilled in the art
The other embodiments being understood that.
Claims (8)
1. independently matching the method for breeding fine breed cow based on embryo biotechnology, which comprises the following steps:
S1: according to the relevant law of country and regulation, introduce international top kind with embryo and it is international top kind with frozen semen；
S2: the number that the kind in S1 is distinguished with embryo and frozen semen, number has uniqueness, and carries out gene one by one
Detection and analysis, reference numeral records all analysis data, and corresponding label makes mutual corresponding electronic tag respectively；
S3: uploading database for the analysis data in S2, make corresponding pre-matching software, by the kind in database with embryo and
Frozen semen data carry out pre- pair analysis, and generate analysis comparison report one by one；
S4: according to the pre- pairing in S3 as a result, choose one or more groups of kinds to be numbered with embryo and frozen semen, and according to correspondence
Number search corresponding kind with embryo and frozen semen；
S5: according to the genetic analysis data in S2, gene group selection is carried out with embryo and frozen semen to the kind of the selection in S4
Or the operation such as monosperm injection intracellular；
S6: being operated to combine kind with embryo and frozen semen by corresponding technical staff and form fertilized eggs, is placed into advanced processing
Receptor cow intrauterine；
S7: the intrauterine embryo of receptor cow carries out sex identification, is decided whether to terminate the pregnant of receptor cow according to appraisal
It is pregnent, when needing terminal pregnancy, carries out the operation of terminal pregnancy, and repeat S6, when being not necessarily to terminal pregnancy, cultivate to receptor mother
Ox childbirth.
2. the method according to claim 1 for independently being matched breeding fine breed cow based on embryo biotechnology, feature are existed
In in S1, donor bull Main Production Rerfomance estimated breeding value or selection index of top kind of the world with frozen semen are most
High ranking be 10% before exporting country within, and Genetic Evaluation high reliablity is in 70%.
3. the method according to claim 1 for independently being matched breeding fine breed cow based on embryo biotechnology, feature are existed
In in S1, top ranked donor cows Main Production Rerfomance estimated breeding value of top kind of the world with embryo is exported country
Within 15% before family.
4. the method according to claim 1 for independently being matched breeding fine breed cow based on embryo biotechnology, feature are existed
In in S2, the electronic tag is RFID label tag.
5. the method according to claim 1 for independently being matched breeding fine breed cow based on embryo biotechnology, feature are existed
In in S2, the number includes letter and number.
6. the method according to claim 5 for independently being matched breeding fine breed cow based on embryo biotechnology, feature are existed
In described kind is W with the number initial of embryo, and the initial of the frozen semen is M.
7. the method according to claim 1 for independently being matched breeding fine breed cow based on embryo biotechnology, feature are existed
In in S3, the comparison report content includes gene information and combination advantage information.
8. the method according to claim 1 for independently being matched breeding fine breed cow based on embryo biotechnology, feature are existed
In, after the receptor cow childbirth target milk cow, the gene and performance shape of record target milk cow, while uploading database.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN112889744A (en) * | 2019-12-04 | 2021-06-04 | 广西贵港秀博基因科技股份有限公司 | Breeding method and breeding system |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112889744A (en) * | 2019-12-04 | 2021-06-04 | 广西贵港秀博基因科技股份有限公司 | Breeding method and breeding system |
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Application publication date: 20190621 |