CN109900767A - A method of utilizing Electrochemical Detection chicken freshness - Google Patents
A method of utilizing Electrochemical Detection chicken freshness Download PDFInfo
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- CN109900767A CN109900767A CN201910288407.1A CN201910288407A CN109900767A CN 109900767 A CN109900767 A CN 109900767A CN 201910288407 A CN201910288407 A CN 201910288407A CN 109900767 A CN109900767 A CN 109900767A
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Abstract
The invention discloses a kind of methods using Electrochemical Detection chicken freshness.The method of the present invention includes following steps: (1) being placed in deionized water and clean to the chicken meat sample of different freshness, then blot moisture, meat is blended, weigh sample and solvent progress ultrasonic extraction is added, filter to get filtrate；(2) filtrate drop coating is dry in glassy carbon electrode surface, it is immersed in buffer solution, with platinum electrode, silver/silver chloride electrode forms three-electrode system, carries out electrochemical response spectrum acquisition to filtrate using linear voltammetry scanning method；(3) electrochemical response spectrum acquisition is carried out according to chicken meat sample of the process in step (2) to unknown freshness；(4) the electrochemical response spectrum of acquisition is subjected to data processing, extracts characteristic value, compares the difference of unknown freshness sample and known freshness sample characteristic value, determine chicken freshness.The advantages that detection method of the invention has operating method simple, is quick on the draw, and instrument cost is low, and speed is fast, and accuracy is high.
Description
Technical field
The invention belongs to technical field of food detection, more particularly to a kind of side using Electrochemical Detection chicken freshness
Method.
Background technique
Chicken is a kind of food of people's daily consumption, containing a large amount of protein and various trace elements, by
Consumers.But it during chicken is from butchering to entering market, is easily infected by bacterial.Especially fresh segmentation
Meat is the natural culture dish of microbe survival and breeding due to surface wettability.Microorganism gathers for a long time on meat surface, can make
It is putrid and deteriorated at meat.Rotten meat not only poor taste, can also cause to seriously endanger to human body, therefore, the inspection to chicken freshness
Measuring tool has important practical value.
Currently, China still generallys use traditional bacteriological analysis method and serological method to the detection of chicken, this two
Kind method and step is cumbersome, reagent price is expensive.In recent years, for the detection of chicken freshness, people have explored many novel
Detection method, such as Computer Vision Detection method, high performance liquid chromatography, nucleic acid probe detection method, biochip test method, hair
Cons electrophoresis Amperometric etc., but the above various detection methods are mostly there is still a need for by laboratory highly sophisticated device, and need
Professional operates, and application aspect is detected on the spot and is had some limitations.
Electrochemical method detection is relatively easy relative to the operation of above-mentioned detection method, does not need using expensive examination
Agent and accurate instrument, are favored in recent years.
For example, Chinese patent CN109470746 discloses a kind of pork freshness detection method, pass through electrochemical sensor
The capacitance of the standard sample of different storage time is detected, pork freshness standard curve is established in acquisition, thus to the new of pork
Freshness is determined.But the above method is needed using electrochemical sensor, and sensor belongs to more accurate electrochemical apparatus, at
This is higher.
Chinese patent CN101806741B disclose a kind of freshness of meat rapid detection method and its detection it is flat
Platform acquires gas by the way that meat to be placed in closed container, using MWNTs/PVA/ (pq) 2Ir (N-phMA) modified electrode to nothing
The case where machine ammonia has good response, then carries out cyclic voltammetric-electrogenerated chemiluminescence scanning, acquisition freshness of meat.But it is above-mentioned
Method is more demanding to experimental implementation, and accuracy is inadequate.
Summary of the invention
Deficiency present in method in view of the above technology utilizes Electrochemical Detection chicken the purpose of the present invention is to provide a kind of
The method of meat freshness.Detection method of the invention has operating method simple, is quick on the draw, and instrument cost is low, and speed is fast, quasi-
The advantages that really property is high.
To achieve the above object, the present invention takes following technical scheme:
A method of using Electrochemical Detection chicken freshness, include the following steps:
(1) sample treatment: being placed in deionized water the chicken meat sample of different freshness and clean, with blotting paper by meat surface
Moisture blots, and meat is blended to meat gruel shape, weighs a certain amount of sample, and a certain amount of solvent is added and carries out ultrasonic extraction, chicken is consolidated
Liquid mixing sample is filtered, and obtains filtrate；
(2) electrochemical response of the chicken meat sample of different freshness composes acquisition: by the filtrate drop coating in a certain amount of step (1)
It is dry in glassy carbon electrode surface, it is immersed in buffer solution, with platinum electrode, silver/silver chloride electrode forms three-electrode system, makes
Electrochemical response spectrum acquisition is carried out with chicken filtrate of the linear voltammetry scanning method to different freshness；
(3) Electrochemical Scanning is carried out to the chicken meat sample that need to be detected: according to the process in step (2) to unknown freshness
Chicken meat sample carries out electrochemical response spectrum acquisition；
(4) electrochemical response of acquisition spectrum is subjected to data processing, extracts characteristic value, compare unknown freshness sample and
The difference for knowing freshness sample characteristic value determines chicken freshness.
Preferably, the amount of step (1) described chicken meat sample is 0.1g~5g.
Preferably, the solvent in step (1) can be water, ethyl alcohol or isopropanol.
Preferably, the additional amount of solvent is 10-50mL in step (1).
Preferably, the ultrasonic extraction time of the chicken in step (1) is 1~5min.
Preferably, filtering filter paper used is polytetrafluoroethylene (PTFE) material in step (1), and pore size is 200nm~2 μm.
Preferably, the drop coating in step (2) and (3) in glassy carbon electrode surface chicken filtrate be 0.5 μ of μ L~3 L.
Preferably, the buffer solution in step (2) and (3) can be molten for phosphate buffer solution, citric acid-sodium citrate buffering
Liquid, NaAc_HAc buffer solution or calmine-hydrochloric acid buffer solution.
Preferably, the linear voltammetric scan method parameter in step (2) and (3) are as follows: sweep interval is -1.5~1.5V, scanning
Speed is 50~100mV/s, and the sampling interval is 1~5mV.
Preferably, step (2) and the concentration of the buffer solution in (3) are 0.1mol/L~0.5mol/L.
Preferably, oxidation peak peak-peak and 0.6V between characteristic value use -0.2V~0.5V of data in step (4)~
The ratio of oxidation peak peak-peak between 1.2V, or using highest in the peak and sweep interval of oxidation peak between 0.4V~0.8V
The ratio of current value.
Between use -0.2V~0.5V of the present invention between oxidation peak peak-peak and 0.6V~1.2V oxidation peak peak-peak ratio
Value, or using maximum current value in the peak and sweep interval of oxidation peak between 0.4V~0.8V ratio as data characteristics
Value, accuracy in detection is high, fast response time, and data calculate simple.
Compared with prior art, the present invention beneficial effect is:
Chicken Noninvasive Measuring Method of Freshness of the invention is caused electrochemical in chicken by same area chicken resting period difference
It is different to learn the electrochemical activity that material exhibits go out, causes the difference of current potential and peak value, to judge to detect the fresh of chicken meat sample
Degree；Chicken Noninvasive Measuring Method of Freshness of the invention is easy to operate, is quick on the draw, and instrument cost is low, can rapidly and accurately detect chicken
The freshness of meat has biggish economic value in practical applications；In addition, also having the advantage that
1, the method testing cost of detection chicken freshness proposed by the present invention is cheap.
2, detection method step of the present invention is simple, requires reviewer low, popularization strong applicability.
3, the method proposed by the present invention using Electrochemical Detection chicken freshness can be developed and can carry chip type electronics and sweep
Device is retouched, and attempts kit, can be used for detecting on the spot.
Specific embodiment
It in order to make the object, technical scheme and advantages of the embodiment of the invention clearer, below will be to the embodiment of the present invention
Technical solution carries out clear, complete description.Obviously, described embodiment is a part of the embodiments of the present invention, rather than
Whole embodiments.Based on described the embodiment of the present invention, those of ordinary skill in the art are without creative work
Under the premise of every other embodiment obtained, belong to protection scope of the present invention.
Unless otherwise defined, technical term or scientific term used in the disclosure are should be in fields of the present invention
The ordinary meaning for thering is the personage of general technical ability to be understood.
Embodiment 1:
The present embodiment is Fresh Grade Breast, chicken wings, the detection of chicken leg freshness, comprising the following steps:
One, the Electrochemical Scanning of chicken different parts difference freshness composes acquisition
(1) 5 DEG C of Fresh Grade Breast on the 0th~10, chicken wings, chicken leg samples will be stored in be placed in deionized water and clean, with water suction
Paper blots Fresh Grade Breast surface moisture；
(2) it takes 1g that will blend by pretreated Fresh Grade Breast to meat gruel shape, 2mL ethyl alcohol is added and mixes and surpasses as solvent
Sound 1min；
(3) it is filtered using the aperture 200nm polytetrafluoroethylene (PTFE) filter paper to sufficient sample is mixed, 1 μ L amount filtrate is taken to drip
It is applied to glassy carbon electrode surface and naturally dry, the electrode after modification is inserted into the phosphate buffer solution of 10mL0.1mol/L, into
Row linear voltammetry Scanning Detction；The wherein condition of Scanning Detction are as follows: sweep interval is -1.0~1.0V, scanning speed is
100mV/s, sampling interval 1mV；The electrochemical response figure of every a sample acquires 5 times.(4) electrochemistry for obtaining scanning is believed
It number is standardized, oxidation peak peak-peak between oxidation peak peak-peak and 0.6V~1.2V between calculating -0.2V~0.5V
Ratio, take the average values of 5 acquisition data, data are as shown in table 1 below:
Table 1 stores the Electrochemical Detection data of the Fresh Grade Breast of different time, chicken wings and chicken leg
For the chicken meat sample of unknown holding time, same method can be taken to be detected, the electrochemistry that will be obtained
Signal is standardized, oxidation peak top between oxidation peak peak-peak and 0.6V~1.2V between calculating -0.2V~0.5V
The ratio of value, so as to evaluate the grade of freshness of the chicken different parts.When only need to two kinds or more of chicken into
When row freshness compares, then ratio is obtained using above-mentioned method of the same race, the biggish sample of ratio is stale.
Electrochemical Detection is carried out using Fresh Grade Breast of the above-mentioned same procedure to 5 DEG C of storage unknown times, extracts its calculating-
Between 0.2V~0.5V between oxidation peak peak-peak and 0.6V~1.2V oxidation peak peak-peak ratio, the results are shown in Table 2.From
As can be seen that the Fresh Grade Breast freshness of sample 2 is minimum in table 2.
Table 2 stores the Fresh Grade Breast Electrochemical Detection data of unknown time
| Sample 1 | Sample 2 | Sample 3 | |
| Ratio | 0.24 | 0.61 | 0.27 |
As seen from the above embodiment, chicken Noninvasive Measuring Method of Freshness of the invention passes through the same area chicken resting period
Difference causes the electrochemical activity that electrochemical substance is shown in chicken different, causes the difference of current potential and peak value, to judge
Detect the freshness of chicken meat sample；Chicken Noninvasive Measuring Method of Freshness of the invention is easy to operate, is quick on the draw, and instrument cost is low,
The freshness of chicken can be rapidly and accurately detected, there is biggish economic value in practical applications.
The method of the present invention that the above embodiments are only used to help understand and its core concept.It should be pointed out that for
For those skilled in the art, without departing from the principle of the present invention, if can also be carried out to the present invention
Dry improvement and modification, these improvement and modification are also fallen into the claims in the present invention protection scope.
Claims (10)
1. a kind of method using Electrochemical Detection chicken freshness, which comprises the steps of:
(1) sample treatment: being placed in deionized water the chicken meat sample of different freshness and clean, with blotting paper by meat surface moisture
It blots, meat is blended to meat gruel shape, weigh a certain amount of sample, a certain amount of solvent is added and carries out ultrasonic extraction, chicken solid-liquid is mixed
It closes sample to be filtered, obtains filtrate；
(2) electrochemical response of the chicken meat sample of different freshness composes acquisition: by the filtrate drop coating in a certain amount of step (1) in glass
Carbon electrodes, it is dry, it is immersed in buffer solution, with platinum electrode, silver/silver chloride electrode forms three-electrode system, uses line
Property voltammetry scanning method electrochemical response spectrum acquisition is carried out to the chicken filtrate of different freshness；
(3) Electrochemical Scanning: the chicken according to the process in step (2) to unknown freshness is carried out to the chicken meat sample that need to be detected
Sample carries out electrochemical response spectrum acquisition；
(4) electrochemical response of acquisition spectrum is subjected to data processing, extracts characteristic value, compare unknown freshness sample and known new
The difference of freshness sample characteristic value, determines chicken freshness.
2. a kind of method using Electrochemical Detection chicken freshness according to claim 1, which is characterized in that step
(1) amount of the chicken meat sample is 0.1g~5g.
3. a kind of method using Electrochemical Detection chicken freshness according to claim 1, which is characterized in that step
(1) solvent in is water, ethyl alcohol or isopropanol.
4. a kind of method using Electrochemical Detection chicken freshness according to claim 1, which is characterized in that step
(1) additional amount of solvent is 10-50mL in.
5. a kind of method using Electrochemical Detection chicken freshness according to claim 1, which is characterized in that step
(1) the ultrasonic extraction time of the chicken in is 1~5min.
6. a kind of method using Electrochemical Detection chicken freshness according to claim 1, which is characterized in that step
(1) filtering filter paper used is polytetrafluoroethylene (PTFE) material in, and pore size is 200nm~2 μm.
7. a kind of method using Electrochemical Detection chicken freshness according to claim 1, which is characterized in that step
(2) and the drop coating in (3) in glassy carbon electrode surface chicken filtrate be 0.5 μ of μ L~3 L.
8. a kind of method using Electrochemical Detection chicken freshness according to claim 1, which is characterized in that step
(2) buffer solution and in (3) is phosphate buffer solution, citric acid-sodium citrate buffer, Acetic acid-sodium acetate buffering are molten
Liquid or calmine-hydrochloric acid buffer solution, the concentration of buffer solution are 0.1mol/L~0.5mol/L.
9. a kind of method using Electrochemical Detection chicken freshness according to claim 1, which is characterized in that step
(2) the linear voltammetric scan method parameter and in (3) are as follows: sweep interval is -1.5~1.5V, and scanning speed is 50~100mV/s,
Sampling interval is 1~5mV.
10. a kind of method using Electrochemical Detection chicken freshness according to claim 1, which is characterized in that step
(4) oxidation peak peak-peak between oxidation peak peak-peak and 0.6V~1.2V between characteristic value use -0.2V~0.5V of data in
Ratio, or the ratio using maximum current value in the peak and sweep interval of oxidation peak between 0.4V~0.8V.
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Cited By (2)
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| CN111272858A (en) * | 2020-04-01 | 2020-06-12 | 杭州电子科技大学 | Analysis method of pottery clay of temple site |
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| CN111579624A (en) * | 2020-05-20 | 2020-08-25 | 杭州电子科技大学 | Method for analyzing storage time of drug-flavor liquor without analyzing compounds |
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