CN109887091A - A method of for lower limb and Thoracolumbar disk fascia to be redeveloped into 3D model - Google Patents
A method of for lower limb and Thoracolumbar disk fascia to be redeveloped into 3D model Download PDFInfo
- Publication number
- CN109887091A CN109887091A CN201910144647.4A CN201910144647A CN109887091A CN 109887091 A CN109887091 A CN 109887091A CN 201910144647 A CN201910144647 A CN 201910144647A CN 109887091 A CN109887091 A CN 109887091A
- Authority
- CN
- China
- Prior art keywords
- fascia
- lower limb
- thoracolumbar disk
- redeveloped
- model
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 210000003195 fascia Anatomy 0.000 title claims abstract description 46
- 238000000034 method Methods 0.000 title claims abstract description 31
- 210000003141 lower extremity Anatomy 0.000 title claims abstract description 26
- 238000004043 dyeing Methods 0.000 claims abstract description 9
- 102000008186 Collagen Human genes 0.000 claims abstract description 8
- 108010035532 Collagen Proteins 0.000 claims abstract description 8
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 8
- 229920001436 collagen Polymers 0.000 claims abstract description 8
- 210000005036 nerve Anatomy 0.000 claims abstract description 8
- LPXQRXLUHJKZIE-UHFFFAOYSA-N 8-azaguanine Chemical compound NC1=NC(O)=C2NN=NC2=N1 LPXQRXLUHJKZIE-UHFFFAOYSA-N 0.000 claims abstract description 6
- 239000000835 fiber Substances 0.000 claims abstract description 6
- 238000000399 optical microscopy Methods 0.000 claims abstract description 6
- 239000000470 constituent Substances 0.000 claims description 5
- 229920001971 elastomer Polymers 0.000 claims description 3
- 239000000806 elastomer Substances 0.000 claims description 3
- 238000009499 grossing Methods 0.000 claims description 3
- 239000004615 ingredient Substances 0.000 claims 1
- 238000010191 image analysis Methods 0.000 abstract description 4
- 238000004140 cleaning Methods 0.000 abstract description 3
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 210000000988 bone and bone Anatomy 0.000 description 2
- 238000005034 decoration Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 210000003205 muscle Anatomy 0.000 description 2
- 238000002560 therapeutic procedure Methods 0.000 description 2
- 210000001519 tissue Anatomy 0.000 description 2
- 208000019901 Anxiety disease Diseases 0.000 description 1
- 102000010834 Extracellular Matrix Proteins Human genes 0.000 description 1
- 108010037362 Extracellular Matrix Proteins Proteins 0.000 description 1
- 230000036506 anxiety Effects 0.000 description 1
- 210000002808 connective tissue Anatomy 0.000 description 1
- 230000009089 cytolysis Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000000280 densification Methods 0.000 description 1
- 210000002744 extracellular matrix Anatomy 0.000 description 1
- 230000003387 muscular Effects 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 230000008520 organization Effects 0.000 description 1
- 239000012188 paraffin wax Substances 0.000 description 1
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The present invention discloses a kind of method for lower limb and Thoracolumbar disk fascia to be redeveloped into 3D model, include the following steps: sample clean: by the ethanol after the corresponding position cleaning of research object with 75%, and sample is placed in spare under sterile dry environment, slice obtains: it is using slice stage property that the lower limb of research object and Thoracolumbar disk fascia are partially sliced, serial section is made with this.The method for being used to lower limb and Thoracolumbar disk fascia being redeveloped into 3D model, pass through azan mallory, the dyeing of van gieson and anti-s100 antibody and the use of DM4500-B optical microscopy (Leica Microsystems) and Paraview software (Kitware Inc.), the utilization of " traveling cube " algorithm, the structure of fascia is analyzed by the way of three-dimensional image analysis, it can be very good to determine collagen in the structural form and sample of fascia, distribution situation is simultaneously stored in computer with data mode (for image format) so as to subsequent research by elasticity and the spatial distribution of nerve fibre.
Description
Technical field
It is specially a kind of for lower limb and Thoracolumbar disk fascia to be redeveloped into 3D model the present invention relates to field of medical technology
Method.
Background technique
Fascia is a kind of fibrous connective tissue of densification, connection muscle, bone and organ, forms one continuously in whole body
Organize network.In human posture's change procedure, it plays an important role to transmitting mechanical force.The artificial fascia of several forms is treated
Method is developed including certain other technologies in muscular fascia lysis and bone therapy operation treatment (OMT), to improve
Stance adjustment and other dynamic (dynamical) expression of muscle skeleton.These therapies and the purpose for the treatment of are to change the mechanical performance of fascia,
Such as density, hardness and viscosity, fascia is made to be more easily adapted to conform to physical stress.In fact, some orthopedists and manual therapist's report
After accusing local organization release, using the fascia area of slow manual force anxiety.These reports have been interpreted to break fascia friendship
Connection is transitioned into the extracellular matrix and other passive viscoplasticity variation fascias of collosol state from gel.
In the prior art, it is analyzed only with structure of the image decoration method to fascia, it is difficult to determine fascia well
Structural form and sample in collagen, elasticity and nerve fibre spatial distribution and by distribution situation with data mode (relative to
For image format) efficiency that research is reduced while increasing research difficulty so as to subsequent research is stored in computer.
Summary of the invention
(1) the technical issues of solving
In view of the deficiencies of the prior art, the present invention provides one kind for lower limb and Thoracolumbar disk fascia to be redeveloped into 3D model
Method, solve in the prior art, analyzed only with structure of the image decoration method to fascia, it is difficult to well determine
Collagen in the structural form and sample of fascia, the spatial distribution of elasticity and nerve fibre and by distribution situation with data mode (phase
For image format) the problem of being stored in computer so as to subsequent research.
(2) technical solution
In order to achieve the above object, the present invention is achieved by the following technical programs: one kind is used for lower limb and Thoracolumbar disk
The method that fascia is redeveloped into 3D model, includes the following steps:
S1, sample clean: by the ethanol after the corresponding position cleaning of research object with 75%, and sample is placed in
It is spare under sterile dry environment；
S2, slice obtain: it is using slice stage property that the lower limb of research object and Thoracolumbar disk fascia are partially sliced, it is made with this
Serial section；
S3, slice dyeing: record the serial section of corresponding lower limb and Thoracolumbar disk fascia, respectively with azan mallory,
The dyeing of van gieson and anti-s100 antibody；
S4, image procossing: the image of lower limb and Thoracolumbar disk fascia is obtained, and is aligned after corresponding result images are merged；
S5, threedimensional model generate: using the portion of required research in the obtained image of color threshold method identification step S2
Point, and generate threedimensional model.
Preferably, in step s 4, used graphical tool is matlab image processing tool packet.
Preferably, in step s 4, image acquisition is connected to DM4500-B optical microscopy (Leica with digital camera
Microsystems) and tiff file is saved as.
Preferably, in step s 5, collagen beam, elastomer and nerve fibre are distinguished using color threshold method in threedimensional model
Dimension, it is after handling all structural constituents, all structural constituents are displayed along, the threedimensional model of tissue is obtained, is led to
Cross Paraview software (Kitware Inc.) realization.
Preferably, in step s 5, " traveling cube " algorithm is used, is extracted as the three-dimensional surface of object to connect it
Vertex and polygon combination, the geometric grid of generation is then refined using smoothing technique.
(3) beneficial effect
The present invention provides a kind of methods for lower limb and Thoracolumbar disk fascia to be redeveloped into 3D model.Have following beneficial
Effect:
The method for being used to lower limb and Thoracolumbar disk fascia being redeveloped into 3D model, passes through azan mallory, van
The dyeing of gieson and anti-s100 antibody and DM4500-B optical microscopy (Leica Microsystems) and
The use of Paraview software (Kitware Inc.), the utilization of " traveling cube " algorithm, using the side of three-dimensional image analysis
Formula analyzes the structure of fascia, can be very good to determine that collagen, elasticity and nerve are fine in the structural form and sample of fascia
Distribution situation is simultaneously stored in computer with data mode (for image format) so as to subsequent by the spatial distribution of dimension
Research, improve the efficiency of research while reducing research difficulty.
Specific embodiment
Described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.Based on the present invention
In embodiment, every other implementation obtained by those of ordinary skill in the art without making creative efforts
Example, shall fall within the protection scope of the present invention.
A method of for lower limb and Thoracolumbar disk fascia to be redeveloped into 3D model, include the following steps:
S1, sample clean: by the ethanol after the corresponding position cleaning of research object with 75%, and sample is placed in
It is spare under sterile dry environment；
S2, slice obtain: it is using slice stage property that the lower limb of research object and Thoracolumbar disk fascia are partially sliced, it is made with this
Serial section (is obtained and is acquired on the foot fascia that four sample two of them samples are the proximal ends after leg, other two sample comes
From Thoracolumbar disk fascia, apart from 7 centimeters of right side third lumbar vertebra spinous process, sample is accurately oriented, and on cardboard, avoids deforming, and is protected
It in the presence of in neutral 10% formalin, is then inserted into paraffin)；
S3, slice dyeing: record the serial section of corresponding lower limb and Thoracolumbar disk fascia, respectively with azan mallory,
The dyeing of van gieson and anti-s100 antibody；
S4, image procossing: the image of lower limb and Thoracolumbar disk fascia is obtained, and is aligned after corresponding result images are merged
(image acquisition is connected to DM4500-B optical microscopy (Leica Microsystems) with digital camera and saves as TIFF text
Part, used graphical tool are matlab image processing tool packet, and all image procossings use public domain image analysis soft
Part ImageJ is executed)；
S5, threedimensional model generate: using the portion of required research in the obtained image of color threshold method identification step S2
Point, and generate threedimensional model and (collagen beam, elastomer and nerve fibre are distinguished using color threshold method in threedimensional model, right
It is after all structural constituents are handled, all structural constituents are displayed along, the threedimensional model of tissue is obtained, is passed through
Paraview software (Kitware Inc.) is realized, uses " traveling cube " algorithm, the three-dimensional surface of object is extracted as
Connect they vertex and polygon combination, the geometric grid of generation is then refined using smoothing technique).
In conclusion the method for being used to lower limb and Thoracolumbar disk fascia being redeveloped into 3D model, by azan mallory,
The dyeing of van gieson and anti-s100 antibody and DM4500-B optical microscopy (Leica Microsystems) and
The use of Paraview software (Kitware Inc.), the utilization of " traveling cube " algorithm, using the side of three-dimensional image analysis
Formula analyzes the structure of fascia, can be very good to determine that collagen, elasticity and nerve are fine in the structural form and sample of fascia
Distribution situation is simultaneously stored in computer with data mode (for image format) so as to subsequent by the spatial distribution of dimension
Research, improve the efficiency of research while reducing research difficulty.
It although an embodiment of the present invention has been shown and described, for the ordinary skill in the art, can be with
A variety of variations, modification, replacement can be carried out to these embodiments without departing from the principles and spirit of the present invention by understanding
And modification, the scope of the present invention is defined by the appended.
Claims (5)
1. a kind of method for lower limb and Thoracolumbar disk fascia to be redeveloped into 3D model, which comprises the steps of:
S1, sample clean: after the corresponding position of research object is cleaned with 75% ethanol, and sample is placed in sterile
It is spare under dry environment；
S2, slice obtain: it is using slice stage property that the lower limb of research object and Thoracolumbar disk fascia are partially sliced, it is made continuously with this
Slice；
S3, slice dyeing: the serial section of corresponding lower limb and Thoracolumbar disk fascia is recorded, uses azan mallory, van respectively
The dyeing of gieson and anti-s100 antibody；
S4, image procossing: the image of lower limb and Thoracolumbar disk fascia is obtained, and is aligned after corresponding result images are merged；
S5, threedimensional model generate: using the part of required research in the obtained image of color threshold method identification step S2, and
Generate threedimensional model.
2. a kind of method for lower limb and Thoracolumbar disk fascia to be redeveloped into 3D model according to claim 1, feature
Be: in step s 4, used graphical tool is matlab image processing tool packet.
3. a kind of method for lower limb and Thoracolumbar disk fascia to be redeveloped into 3D model according to claim 1, feature
Be: in step s 4, image acquisition is connected to DM4500-B optical microscopy (Leica with digital camera
Microsystems) and tiff file is saved as.
4. a kind of method for lower limb and Thoracolumbar disk fascia to be redeveloped into 3D model according to claim 1, feature
It is: in step s 5, collagen beam, elastomer and nerve fibre is distinguished using color threshold method in threedimensional model, to institute
It is after organized ingredient is handled, all structural constituents are displayed along, the threedimensional model of tissue is obtained, Paraview is passed through
Software (Kitware Inc.) is realized.
5. a kind of method for lower limb and Thoracolumbar disk fascia to be redeveloped into 3D model according to claim 1, feature
Be: in step s 5, using " traveling cube " algorithm, by the three-dimensional surface of object be extracted as connecting they vertex and
Then the combination of polygon refines the geometric grid of generation using smoothing technique.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910144647.4A CN109887091A (en) | 2019-02-27 | 2019-02-27 | A method of for lower limb and Thoracolumbar disk fascia to be redeveloped into 3D model |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910144647.4A CN109887091A (en) | 2019-02-27 | 2019-02-27 | A method of for lower limb and Thoracolumbar disk fascia to be redeveloped into 3D model |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109887091A true CN109887091A (en) | 2019-06-14 |
Family
ID=66929601
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910144647.4A Pending CN109887091A (en) | 2019-02-27 | 2019-02-27 | A method of for lower limb and Thoracolumbar disk fascia to be redeveloped into 3D model |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109887091A (en) |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2003023484A1 (en) * | 2001-09-10 | 2003-03-20 | John Petrali | Free-floating cryostat sections for immunoelectron microscopy |
| CN101915693A (en) * | 2010-07-07 | 2010-12-15 | 新疆医科大学 | Human embryonic trigeminus based three-dimensional reconstruction method by using histotomy staining |
| CN106408648A (en) * | 2015-08-03 | 2017-02-15 | 青岛海信医疗设备股份有限公司 | Medical-tissue slice-image three-dimensional reconstruction method and equipment thereof |
| CN108022291A (en) * | 2017-12-01 | 2018-05-11 | 中山大学附属第医院 | The visualization of 3 d method for reconstructing of human peripheral nerve beamforming structure |
| CN109087390A (en) * | 2018-09-30 | 2018-12-25 | 金华市中心医院 | Micro- nerve digitlization and three-dimensional nerve network establishing method around the big organ of pelvic cavity |
-
2019
- 2019-02-27 CN CN201910144647.4A patent/CN109887091A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2003023484A1 (en) * | 2001-09-10 | 2003-03-20 | John Petrali | Free-floating cryostat sections for immunoelectron microscopy |
| CN101915693A (en) * | 2010-07-07 | 2010-12-15 | 新疆医科大学 | Human embryonic trigeminus based three-dimensional reconstruction method by using histotomy staining |
| CN106408648A (en) * | 2015-08-03 | 2017-02-15 | 青岛海信医疗设备股份有限公司 | Medical-tissue slice-image three-dimensional reconstruction method and equipment thereof |
| CN108022291A (en) * | 2017-12-01 | 2018-05-11 | 中山大学附属第医院 | The visualization of 3 d method for reconstructing of human peripheral nerve beamforming structure |
| CN109087390A (en) * | 2018-09-30 | 2018-12-25 | 金华市中心医院 | Micro- nerve digitlization and three-dimensional nerve network establishing method around the big organ of pelvic cavity |
Non-Patent Citations (2)
| Title |
|---|
| 来茂德: "《病理学高级教程》", 28 February 2015 * |
| 陈增淦: "人体臂丛神经连续组织切片计算机辅助三维重建研究", 《中国优秀博硕士学位论文全文数据库(电子期刊) 医药卫生科技辑》 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Stecco et al. | Fascia redefined: anatomical features and technical relevance in fascial flap surgery | |
| Hu et al. | Is the psoas a hip flexor in the active straight leg raise? | |
| EP3123962A3 (en) | Patient specific knee alignment guide | |
| WO2005112563A3 (en) | Virtual surgical system and methods | |
| Ben-Zvi et al. | 3D architecture of trabecular bone in the pig mandible and femur: inter-trabecular angle distributions | |
| Porpino et al. | A new cingulate (Mammalia: Xenarthra), Pachyarmatherium brasiliense sp. nov., from the Late Pleistocene of Northeastern Brazil | |
| SHULTZ | Muscular anatomy of a whipspider, Phrynus longipes (Pocock)(Arachnida: Amblypygi), and its evolutionary significance | |
| CN102646350A (en) | Centrum location device for virtual surgery force sense information acquisition | |
| Blasi et al. | Multidisciplinary approach to the persistent double distal tendon of the biceps brachii | |
| Pirri et al. | Elastic Fibres in the subcutaneous tissue: Is there a difference between superficial and muscular fascia? A cadaver study | |
| Kumar et al. | Determination of Spearman correlation coefficient (r) to evaluate the linear association of dermal collagen and elastic fibers in the perspectives of skin injury | |
| CN109887091A (en) | A method of for lower limb and Thoracolumbar disk fascia to be redeveloped into 3D model | |
| Wolfe et al. | Load-displacement behavior in a distal radial fracture model. The effect of simulated healing on motion | |
| CN202027692U (en) | Clamping device for vertebral body specimen | |
| Bartlett | Periosteal lipoma: Report of two cases | |
| Hamilton | Chapter two Ritual Killing, Mutilation, and Dismemberment at Huaca de la Luna: Sharp Force Trauma Among Moche Sacrifice Victims in Plazas 3A and 3C | |
| CN109003524B (en) | Method for preparing skeletal muscle specimen | |
| Trewartha | What lies beneath? | |
| CN205386157U (en) | Drag hook for bone surgery | |
| Manning | The vertebral endplate: its structure and function | |
| Gassner et al. | The “Segmental SMAS Model”: A Didactic Concept of Face and Neck Lift Anatomy | |
| RU2477983C2 (en) | Intra-articular osteosynthesis guide pin | |
| CN201572197U (en) | Artificial bone grafting operation kit | |
| Hendry et al. | Structural comparison of the finger proximal interphalangeal joint surfaces and those of the third toe: suitability for joint reconstruction | |
| Hurtado-Macías et al. | Assessing mechanical behavior of ostrich and equine trabecular and cortical bone based on depth sensing indentation measurements |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20190614 |