CN109884041B - Pesticide residue short-term test card - Google Patents
Pesticide residue short-term test card Download PDFInfo
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- CN109884041B CN109884041B CN201910114111.8A CN201910114111A CN109884041B CN 109884041 B CN109884041 B CN 109884041B CN 201910114111 A CN201910114111 A CN 201910114111A CN 109884041 B CN109884041 B CN 109884041B
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Abstract
The invention provides a rapid pesticide residue detection card, which comprises a front side and a back side, wherein the front side comprises a reaction plate 1, a detection plate and a quality control plate, the reaction plate 1, the detection plate and the quality control plate are sequentially connected, and press lines are arranged at the connected parts; the back is provided with a reaction plate 2 arranged on the back of the reaction plate 1. The rapid test card provided by the invention simultaneously comprises the detection sheet and the quality control sheet, so that a sample to be tested and a control sample can be simultaneously detected, the accuracy of a result is greatly improved, and the workload for detecting pesticide residues is reduced. When the rapid test card is used, a system of acetylcholinesterase and indoleacetic ester is used, so that professional personnel and equipment are not needed, the pesticide residue of a sample can be rapidly and simply detected, and the detection time and cost of the pesticide residue are shortened.
Description
Technical Field
The invention relates to the technical field of pesticide residue detection, in particular to a rapid pesticide residue detection card.
Background
At present, organophosphorus and carbamate pesticides are widely used in the production process of vegetables and fruits in China, and because the action mechanisms of the organophosphorus and carbamate pesticides achieve the insecticidal effect by inhibiting the activity of acetylcholinesterase, if the pesticides are abused or improperly used, the residual pesticides in the vegetables and fruits can seriously affect the health of people, so that the monitoring of the pesticide residues in the vegetables and fruits becomes very important.
Gas phase or liquid phase chromatography is a relatively precise method for detecting pesticide residue at present, and the method for rapidly detecting the pesticide residue mainly comprises the steps of using a pesticide residue rapid detection card, generally based on the chemical reaction of acetylcholinesterase and a chromogenic substance, and determining the pesticide residue by comparing the color shade difference with a color comparison card.
However, gas or liquid chromatography requires specialized equipment and specialized personnel. The common pesticide residue rapid test card is used for single sample detection, and a new rapid test card is required to be additionally used for detecting a comparison sample, so that the workload during detection is increased, and misjudgment of a detection result is possibly caused by a certain time difference between the detection of the detection sample and the detection of the comparison sample and the difference between the rapid test cards.
Disclosure of Invention
In order to solve the technical problems, the invention provides the rapid test card which is simple and convenient to operate, can simultaneously detect a sample by comparing the sample with the sample at one time, has high sensitivity, is easy to carry, and can carry out on-site real-time detection.
The invention is realized by the following technical scheme: a pesticide residue rapid detection card comprises a front side and a back side, wherein the front side comprises a reaction plate 1, a detection plate and a quality control plate, the reaction plate 1, the detection plate and the quality control plate are sequentially connected, and press lines are arranged at the connected parts; the back is provided with a reaction plate 2 arranged on the back of the reaction plate 1.
Furthermore, acetylcholinesterase is fixed on the detection sheet and the quality control sheet, and indoleacetic acid ester is fixed on the reaction sheet 1 and the reaction sheet 2.
Furthermore, the reaction plate 1, the reaction plate 2, the detection plate and the quality control plate are folded in half through pressing lines and then are overlapped with each other, the reaction plate 1 is in contact with the detection plate, and the quality control plate is in contact with the reaction plate 2.
Furthermore, the reaction sheet 1, the reaction sheet 2, the detection sheet and the quality control sheet are all provided with tablets, and the tablets of the detection sheet and the quality control sheet contain acetylcholinesterase; the tablets of reaction sheets 1 and 2 contained indole acetate.
Further, the tablet is circular and has a diameter of 11.5 to 12.5 mm.
Further, the pesticide residue rapid detection card is a PP paperboard.
Further, the PP paperboard has the total length of 63-67mm, the width of 20-24mm and the thickness of 0.25-0.4 mm.
Further, the reaction plate 1, the detection plate and the quality control plate are respectively composed of three octagons and are sequentially connected.
Furthermore, the detection plate and the quality control plate are regular octagons, and the reaction plate 1 is an irregular octagon.
Further, the distance from the leftmost side of the reaction plate 1 to the edge of the tablet is 4mm, and the distances from the other seven sides to the tablet are all 5 mm.
The invention has the beneficial effects that: when the rapid test card is used, a system of acetylcholinesterase and indoleacetic ester is used, so that professional personnel and equipment are not needed, the pesticide residue of a sample can be rapidly and simply detected, and the detection time and cost of the pesticide residue are shortened. The rapid test card provided by the invention contains the detection sheet and the quality control sheet at the same time, so that a sample to be tested and a control sample can be simultaneously detected, the accuracy of a result is greatly improved, and the workload for detecting pesticide residues is reduced.
Drawings
FIG. 1 is a front view of the rapid pesticide residue detection card of the present invention;
FIG. 2 is a back view of the rapid pesticide residue detection card of the present invention;
FIG. 3 is a graph showing the results of the detection in example 2;
FIG. 4 is a graph showing the results of detection in example 3;
FIG. 5 is a graph showing the results of the detection in example 4;
FIG. 6 is a diagram showing the results of detection in example 5.
Detailed Description
In order to facilitate understanding of those skilled in the art, the present invention will be further described with reference to the following embodiments and the accompanying drawings.
Example 1
As shown in fig. 1 and 2, the invention relates to a rapid pesticide residue detection card, which comprises a front surface and a back surface, wherein the front surface comprises a reaction plate 1, a detection plate and a quality control plate, the reaction plate 1, the detection plate and the quality control plate are sequentially connected, and press lines are arranged at the connected parts; the back is provided with a reaction plate 2 arranged on the back of the reaction plate 1.
In this embodiment, both the detection plate and the quality control plate are fixed with acetylcholinesterase, and both the reaction plate 1 and the reaction plate 2 are fixed with indoleacetic acid ester.
In this embodiment, the reaction plate 1, the reaction plate 2, the detection plate and the quality control plate are folded in half by pressing lines and then overlapped with each other, the reaction plate 1 contacts with the detection plate, and the quality control plate contacts with the reaction plate 2.
In this embodiment, the reaction sheet 1, the reaction sheet 2, the detection sheet and the quality control sheet are all provided with tablets, and the tablets of the detection sheet and the quality control sheet contain acetylcholinesterase; the tablets of reaction sheets 1 and 2 contained indole acetate.
In this example, the tablets are circular and have a diameter of 12 mm.
In this embodiment, the pesticide residue short-term test card is PP cardboard.
In this embodiment, the total length of the PP paperboard is 65mm, the width is 22mm, and the thickness is 0.3 mm.
In this embodiment, the reaction plate 1, the detection plate and the quality control plate are respectively composed of three octagons, and are sequentially connected.
In this embodiment, the detection wafer and the quality control wafer are regular octagons, and the reaction wafer 1 is an irregular octagon.
In this embodiment, the distance from the leftmost side of the reaction plate 1 to the edge of the tablet is 4mm, and the distances from the other seven sides to the tablet are all 5 mm.
The quick detection card for pesticide residue of the invention has the following specific using process: when pesticide residue is detected, a detection sample and a control sample are respectively dripped on a detection sheet and a quality control sheet, the detection sheet and a reaction sheet 1 are folded through pressing lines, so that the detection sheet is overlapped and contacted with tablets on the reaction sheet 1, and then the quality control sheet and the reaction sheet 2 are folded in half, so that the quality control sheet is overlapped and contacted with tablets of the reaction sheet 2. The detection piece and the quality control piece are arranged on the same rapid test card, and can simultaneously detect a sample to be detected and a control sample.
Example 2 detection of pesticide residue in vegetable without excessive pesticide by surface wiping method
(1) Wiping off soil on the surface of mustard which has no pesticide exceeding the standard on the market, sucking eluent by using a suction pipe, slightly dropping 3-4 drops of eluent on the surface of the vegetable, and then slightly rubbing the other piece of vegetable at a liquid drop position.
(2) Taking a piece of quick measuring card, and dripping all liquid drops on the vegetables on a white detection piece of the quick measuring card.
(3) 2 drops of clean eluent are gently dropped on the white quality control chip on the rapid-determination card by a pipette.
(4) Firstly, the folding line between the detection piece and the reaction piece 1 is folded, the white detection piece is contacted with the red reaction piece 1, and then the folding line between the quality control piece and the reaction piece 2 is folded again, so that the quality control piece is contacted with the reaction piece 2.
(5) And pinching for 3 minutes by hand.
(6) And opening the rapid test card, and observing the colors of the quality control chip and the detection chip after waiting for 5-15 minutes.
And (3) detection results: as shown in FIG. 3, the "quality control chip" turns blue, the "detection chip" is similar to the "quality control chip" in blue, and the result is negative, which indicates that the detected mustard pesticide residue does not exceed the standard.
Example 3 Integrated test method for detecting pesticide residue of vegetable without pesticide exceeding standard
(1) The method comprises the steps of wiping off soil on the surface of the mustard which has no pesticide exceeding the standard on the market, and tearing the mustard by hands or cutting the mustard into mustard leaves with the size of about 1 multiplied by 1cm by using scissors. Adding 20 leaves onto a washing bottle, adding 2ml of eluent by using a pipette, slightly shaking for 50 times, and waiting for 2 minutes.
(2) A piece of rapid test card is taken, and 2 drops of clean eluent are gently dripped onto a white quality control chip on the rapid test card by a pipette.
(3) The vegetable soaking solution in the washing bottle is absorbed by a dropper, and 2 drops are lightly dropped on a white detection piece of the quick detection card.
(4) Firstly, folding the fold line between the detection sheet and the reaction sheet 1 to make the detection sheet contact with the reaction sheet 1, and then folding the fold line between the red reaction sheets 2 of the quality control sheet to make the quality control sheet contact with the reaction sheet 2.
(5) And pinching for 3 minutes by hand.
(6) And opening the rapid test card, and observing the colors of the quality control chip and the detection chip after waiting for 5-15 minutes.
And (3) detection results: as shown in FIG. 4, the quality control wafer turns blue, the detection wafer is deeper than the quality control wafer, and the result is negative, which indicates that the detected mustard pesticide residue does not exceed the standard.
Example 4 detection of pesticide residue in vegetables with overproof pesticide by surface wiping method
The method of example 1 was used to detect the commercial superscalar pesticide mustard.
And (3) detection results: as shown in FIG. 5, the quality control tablet is changed into blue, the detection tablet is lighter than the quality control tablet in blue, and the result is positive, which indicates that the detected mustard pesticide is superscalar but not serious, but the superscalar concentration is still harmful to human bodies.
Example 5 Overall test method for detecting pesticide residue in vegetable with pesticide exceeding standard
The method of example 2 was used to detect the superscalar mustard pesticide on the market.
And (3) detection results: as shown in FIG. 6, the quality control tablet turns blue, the detection tablet does not turn blue, and the result is positive, which indicates that the detected mustard pesticide seriously exceeds the standard.
The above examples show that the pesticide residue rapid detection card can rapidly detect the pesticide residue in vegetables, and is accurate in result and convenient to carry.
The above-mentioned embodiments only express several embodiments of the present invention, and the description thereof is more specific and detailed, but not construed as limiting the scope of the invention. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the inventive concept, which falls within the scope of the present invention. Therefore, the protection scope of the present patent shall be subject to the appended claims.
Claims (8)
1. A pesticide residue rapid detection card is characterized by comprising a front side and a back side, wherein the front side comprises a reaction plate 1, a detection plate and a quality control plate, the reaction plate 1, the detection plate and the quality control plate are sequentially connected, and press lines are arranged at the connected parts; the back surface is provided with a reaction plate 2 which is arranged on the back surface of the reaction plate 1; acetylcholinesterase is fixed on the detection sheet and the quality control sheet, and indoleacetic ester is fixed on the reaction sheet 1 and the reaction sheet 2; the reaction plate 1, the reaction plate 2, the detection plate and the quality control plate are folded in half through pressing lines and then are overlapped with each other, the reaction plate 1 is in contact with the detection plate, and the quality control plate is in contact with the reaction plate 2.
2. The rapid detection card for pesticide residues as claimed in claim 1, wherein the reaction sheet 1, the reaction sheet 2, the detection sheet and the quality control sheet are provided with tablets, and the tablets of the detection sheet and the quality control sheet contain acetylcholinesterase; the tablets of reaction sheets 1 and 2 contained indole acetate.
3. The rapid pesticide residue test card of claim 2, wherein the tablet is circular and has a diameter of 11.5 to 12.5 mm.
4. The rapid pesticide residue detection card of any one of claims 1 to 3, wherein the rapid pesticide residue detection card is a PP cardboard.
5. The rapid pesticide residue detection card of claim 4, wherein the PP paper board has a total length of 63-67mm, a width of 20-24mm and a thickness of 0.25-0.4 mm.
6. The rapid pesticide residue detection card of any one of claims 1 to 3 or 5, wherein the reaction plate 1, the detection plate and the quality control plate are respectively composed of three octagons and are connected in sequence.
7. The rapid pesticide residue detection card of claim 6, wherein the detection sheet and the quality control sheet are regular octagons, and the reaction sheet 1 is irregular octagons.
8. The rapid pesticide residue detection card of claim 7, wherein the distance from the leftmost side of the reaction plate 1 to the edge of the tablet is 4mm, and the distances from the other seven sides to the tablet are 5 mm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910114111.8A CN109884041B (en) | 2019-02-14 | 2019-02-14 | Pesticide residue short-term test card |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201910114111.8A CN109884041B (en) | 2019-02-14 | 2019-02-14 | Pesticide residue short-term test card |
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| CN109884041A CN109884041A (en) | 2019-06-14 |
| CN109884041B true CN109884041B (en) | 2021-04-06 |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB2046577B (en) * | 1978-06-22 | 1982-12-15 | Freyberg Chem Fab Werner | Method and apparatus for fumigating |
| CN1734257A (en) * | 2004-08-10 | 2006-02-15 | 中国科学院上海生命科学研究院 | Vegetable pesticide residue speed is surveyed paper card and preparation method thereof |
| CN101319997A (en) * | 2007-06-08 | 2008-12-10 | 广州绿洲生化科技有限公司 | Card suitable for fast detecting pesticide residue in tea and detection method thereof |
| CN203672793U (en) * | 2014-01-15 | 2014-06-25 | 王建忠 | Pesticide residue detection test paper |
| CN206399840U (en) * | 2016-11-08 | 2017-08-11 | 郑州贝贝生物科技有限公司 | Detect the Rapid detection test strip of Organophosphorus and carbamate pesticides pesticide residue |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106872706B (en) * | 2017-01-25 | 2019-01-04 | 中国农业科学院油料作物研究所 | It is a kind of for detecting the fluorescence polarization immunoassay method of carbaryl |
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2019
- 2019-02-14 CN CN201910114111.8A patent/CN109884041B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB2046577B (en) * | 1978-06-22 | 1982-12-15 | Freyberg Chem Fab Werner | Method and apparatus for fumigating |
| CN1734257A (en) * | 2004-08-10 | 2006-02-15 | 中国科学院上海生命科学研究院 | Vegetable pesticide residue speed is surveyed paper card and preparation method thereof |
| CN101319997A (en) * | 2007-06-08 | 2008-12-10 | 广州绿洲生化科技有限公司 | Card suitable for fast detecting pesticide residue in tea and detection method thereof |
| CN203672793U (en) * | 2014-01-15 | 2014-06-25 | 王建忠 | Pesticide residue detection test paper |
| CN206399840U (en) * | 2016-11-08 | 2017-08-11 | 郑州贝贝生物科技有限公司 | Detect the Rapid detection test strip of Organophosphorus and carbamate pesticides pesticide residue |
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