CN109884011A - Based on carboxyl chitosan/dithiothreitol (DTT)-gold nano cluster doxorubicin fluorescence detection method - Google Patents
Based on carboxyl chitosan/dithiothreitol (DTT)-gold nano cluster doxorubicin fluorescence detection method Download PDFInfo
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Abstract
The present invention discloses a kind of based on carboxyl chitosan/dithiothreitol (DTT)-gold nano cluster doxorubicin fluorescence detection method.Strong photo induced electron transfer occurs after specificity interaction using gold nano cluster and adriamycin in it, causes the fluorescence of gold nano cluster to quench, to realize the measurement of adriamycin.Adriamycin setting-out line range is 0.05 ~ 2 μm of ol/L, and lowest detection is limited to 0.005 μm of ol/L.The preparation of gold nano cluster used in new detecting method simplicity is easy to get, and has many advantages, such as that easy to operate, detection time is short, high sensitivity, high specificity, use easy to spread.
Description
Technical field
The present invention is based on carboxyl chitosan/dithiothreitol (DTT)-fluorescent au nanocluster materials, provide a kind of adriamycin
(Doxorubicin) quick and overdelicate detection method, belongs to analytical chemistry and field of nanometer technology.
Background technique
It is known that adriamycin is anthraquinone analog compound, it is widely used in the treatment for the treatment of leukaemia, breast cancer etc..Although Ah
Mycin has proved to be a kind of active drug, but its therapeutic index is low, long-term administration or dosage is slightly larger to cause
Serious side effect, including bone marrow suppression and cardiac toxic.
Many analysis methods, including high performance liquid chromatography, capillary electrophoresis, mass spectrum, electrochemical method etc., all by
Report can be used for the detection of adriamycin.But all there is certain defects, such as expensive instrument and toxic examination in these methods
Agent, complicated pretreatment process, poor reproducibility and response time length etc..Therefore, simple, accurate, sensitive, quick survey is developed
The method for determining adriamycin is quite important.
Gold nano cluster (gold nanoclusters, AuNCs) is a kind of novel fluorescent nano material, with ruler
Very little small, nontoxic, good water solubility, good light stability, Stokes displacement is big, large specific surface area, preparation condition are mild, surface is easy to repair
It is decorated with and is research hotspot in recent years outstanding advantages of photoluminescent property is with size adjustable, be widely used in catalysis, passed
Feel the fields such as detection, Nanoparticle labeling, medical imaging and photoelectronics.
The present invention provides one using carboxyl chitosan/dithiothreitol (DTT)-fluorescent au nanocluster material as fluorescence probe
The new method of easy, the sensitive adriamycin detection of kind.
Summary of the invention
Deficiency in view of the above-mentioned prior art, the purpose of the present invention is to provide one kind to be based on carboxyl chitosan/bis-
Sulphur threitol-gold nano cluster doxorubicin fluorescence detection method.It revives including using adriamycin and carboxyl chitosan/bis- sulphur
Strong photo induced electron transfer effect between sugar alcohol-gold nano cluster, quenches the fluorescence of gold nano cluster, realizes fast to adriamycin
Speed, Sensitive Detection, and it is used for the detection of actual sample.
In order to realize the purpose of above-mentioned detection method, the invention adopts the following technical scheme:
It is a kind of based on carboxyl chitosan/dithiothreitol (DTT)-gold nano cluster doxorubicin fluorescence detection method, it is characterized in that utilizing
Strong photo induced electron transfer occurs after the specificity interaction of gold nano cluster and adriamycin, leads to the fluorescence of gold nano cluster
It quenches, to realize the measurement of adriamycin；The gold nano cluster is made of following steps: being by 1 mL concentration
0.4 mol/L sodium hydroxide solution and 1.6 mL concentration are that 20 mg/mL gold chlorides are pre-mixed, and 7.4 mL concentration are then added
The dithiothreitol (DTT) solution for being 0.1 mol/L for 50 mg/mL carboxyl chitosan solution and 10 mL concentration, shakes up and is placed on 37 DEG C
Obtain carboxyl chitosan/dithiothreitol (DTT)-gold nano cluster within isothermal reaction 8 hours in water-bath；By the gold nano group after reaction
Cluster is dialysed 24 hours with the bag filter of molecular cut off 3500 in distilled water, obtains carboxyl chitosan/bis- sulphur Soviet Union after purification
Sugar alcohol-gold nano cluster.
It is described a kind of based on carboxyl chitosan/dithiothreitol (DTT)-gold nano cluster doxorubicin fluorescence detection method,
It is characterized in judge the concentration of adriamycin according to the fluorescence intensity at 645 nm of maximum emission wavelength of fluorescence spectrum.
It is described a kind of based on carboxyl chitosan/dithiothreitol (DTT)-gold nano cluster doxorubicin fluorescence detection method,
The determination step of feature adriamycin is as follows: 0.1 mL carboxyl chitosan/dithiothreitol (DTT)-gold nano cluster solution being taken to be added to
The concentration of 1.9 mL adriamycins containing various concentration is 20 mmol/L, in the phosphate buffer of pH=6.0, is uniformly mixed；Reaction
After measure its excitation wavelength at 645 nm be 285 nm fluorescence intensity level F；According to blank fluorescence intensity value F0With
Ratio (the F of fluorescence intensity F containing adriamycin0/ F) draw standard curve quantified；Adriamycin setting-out line range is
0.05 ~ 2 μm of ol/L, lowest detection are limited to 0.005 μm of ol/L.
A kind of carboxyl chitosan/dithiothreitol (DTT)-gold nano cluster that is based on of the present invention quickly measures blood serum sample
The method of middle adriamycin, it is characterized in that including the following steps: to take 0.1 mL carboxyl chitosan/dithiothreitol (DTT)-gold nano cluster
Solution is added in 1.9 mL human serum sample's solution, is uniformly mixed, is measured its excitation wave at 645 nm after reaction
The fluorescence intensity level F of a length of 285 nm；The standard curve measured in conjunction with adriamycin calculates the content of adriamycin, human serum sample's
For recovery of standard addition 95.7 ~ 102.5%, relative standard deviation is 1.12 ~ 1.62%.
The gold nano cluster is made of following steps: by 1 mL concentration be 0.4 mol/L sodium hydroxide solution and
1.6 mL concentration are that 20 mg/mL gold chlorides are pre-mixed, and it is 50 mg/mL carboxyl chitosan solution that 7.4 mL concentration, which are then added,
The dithiothreitol (DTT) solution for being 0.1 mol/L with 10 mL concentration shakes up and is placed in 37 DEG C of water-baths isothermal reaction 8 hours
To carboxyl chitosan/dithiothreitol (DTT)-gold nano cluster；By the dialysis of molecular cut off 3500 of the gold nano cluster after reaction
Bag is dialysed 24 hours in distilled water, obtains carboxyl chitosan/dithiothreitol (DTT)-gold nano cluster after purification.
Specifically, the present invention adopts the following technical solutions:
(1) preparation of gold nano cluster
1 mL sodium hydroxide (0.4 mol/L) and 1.6 mL gold chlorides (20 mg/mL) are pre-mixed, 7.4 mL carboxylations are added
Chitosan solution (50 mg/mL) mixes, and 10 mL dithiothreitol (DTT)s (0.1 mol/L) then is added, and it is small that 37 DEG C of water-baths are incubated for 8
When.Reaction solution is taken out, is dialysed in distilled water 24 hours with the bag filter that molecular cut off is 3500, obtains carboxylation after purification
Chitosan/dithiothreitol (DTT)-gold nano cluster.All glasswares used in preparation process pass through chloroazotic acid immersion, are used in combination
Distilled water thoroughly cleans, and dries.
(2) measurement of adriamycin
Gold nano cluster solution prepared by 0.1 mL step (1) is added to the phosphate of 1.9 mL adriamycins containing various concentration
In buffer (20 mmol/L, pH=6.0), measuring its fluorescence intensity level F(excitation wavelength at 645 nm after mixing is
285 nm).According to F0(blank) and F(contain adriamycin) ratio (F0/ F) draw standard curve quantified.Adriamycin measurement
The range of linearity be 0.05 ~ 2 μm of ol/L, lowest detection is limited to 0.005 μm of ol/L.
Advantages of the present invention:
(1) present invention is occurred after being interacted using carboxyl chitosan/dithiothreitol (DTT)-gold nano cluster and adriamycin specificity
Photo induced electron transfer, so that the fluorescence of gold nano cluster is suppressed, to show the variation of fluorescence spectral characteristic, Ke Yiyong
In the content detection of adriamycin.
(2) carboxyl chitosan/dithiothreitol (DTT)-gold nano cluster used in the present invention, preparation process is simple and quick,
Quantum yield is high.
(3) present invention is low to the processing requirement of sample, high to the detection specificity of adriamycin, and method is easy, quick.
(4) the detection range of linearity of present invention detection adriamycin is 0.05 ~ 2 μm of ol/L, the high sensitivity of detection, detection
Be limited to 0.005 μm of ol/L, be better than or close to other detection methods sensitivity.In addition, recovery of standard addition 95.7% ~
Between 102.5%, it can be used for actual sample detection.
Measuring method of the invention can go out the concentration level of adriamycin in sample by fluorescent spectrophotometer assay,
Measurement sensitivity is high, and specificity is good, and accuracy is good.Detection process is easy, and stability is good, have easy to operate, detection time is short,
The advantages that high sensitivity, high specificity, use easy to spread.
Detailed description of the invention
Fig. 1 is carboxyl chitosan/dithiothreitol (DTT)-gold nano cluster fluorescence emission spectrogram of compound.
Fig. 2 is that carboxyl chitosan/dithiothreitol (DTT)-gold nano cluster is had an effect with the adriamycin that concentration is 5 μm of ol/L
Fluorescence emission spectrogram of compound afterwards.
Fig. 3 is that carboxyl chitosan/dithiothreitol (DTT)-gold nano cluster is had an effect with the adriamycin that concentration is 5 μm of ol/L
Before (A) and the rear appearance comparative diagram of (B) under ultraviolet light irradiation.
Fig. 4 is to quench carboxyl chitosan/dithiothreitol (DTT)-gold nano cluster fluorescence intensity shadow to adriamycin in the reaction time
Ring figure.Ordinate indicates F0(blank) and F(contain adriamycin) ratio.
Fig. 5 is that system pH quenches carboxyl chitosan/dithiothreitol (DTT)-gold nano cluster fluorescence intensity to adriamycin
Influence diagram.Ordinate indicates F0(blank) and F(contain adriamycin) ratio.
Fig. 6 is carboxyl chitosan/dithiothreitol (DTT)-gold nano cluster fluorescence emission spectrum in the presence of various concentration adriamycin
Figure.Doxorubicin concentration is followed successively by 0,0.05,0.1,0.2,0.4,0.6,0.8,1.0,1.5,2.0,2.5 and 5.0 μ from top to bottom
mol/L。
Fig. 7 is the canonical plotting of adriamycin measurement, and ordinate indicates F0(blank) and F(contain adriamycin) ratio.
Fig. 8 is the interference experiment of adriamycin detection, compared 13 kinds of different interfering substances in addition to adriamycin, indulges and sit
Mark indicates F0(blank) and F(contain interfering substance) ratio relation figure.Abscissa 0 ~ 14 respectively represents blank, adriamycin, Zn2+、
Ca2+、HCO3 -、K+、Na+、Mg2+、Fe3+、Fe2+, glutathione, cysteine, cytarabine, Ismipur and cyclophosphamide.
Specific embodiment
One aspect of the present invention be to provide it is a kind of based on carboxyl chitosan/dithiothreitol (DTT)-gold nano cluster Ah
The fluorescence detection method of mycin.Including photic using adriamycin and carboxyl chitosan/dithiothreitol (DTT)-gold nano cluster
Electronics transfer effect, inhibits the fluorescence of gold nano cluster, with the increase of doxorubicin concentration, fluorophor ties up to maximum emission wavelength
Fluorescence intensity (F) at 645 nm reduces.It takes in a series of standard sample addition systems containing various concentration adriamycin and reacts,
According to the fluorescence intensity F at 645 nm of maximum emission wavelength0(blank) and F(contain adriamycin) ratio (F0/ F) draw standard
Curve, to realize the detection of adriamycin.
The technical solution of detection method is further described below in conjunction with attached drawing and several embodiments.
Embodiment 1:
By 1 mL concentration be 0.4 mol/L sodium hydroxide solution and 1.6 mL concentration are that 20 mg/mL gold chlorides are pre-mixed, and
It is molten that the dithiothreitol (DTT) that 7.4 mL concentration are 50 mg/mL carboxyl chitosan solution and 10 mL concentration are 0.1 mol/L is added afterwards
Liquid, shakes up to be placed on isothermal reaction 8 hours in 37 DEG C of water-baths and obtains carboxyl chitosan/dithiothreitol (DTT)-gold nano cluster.It will
Gold nano cluster after reaction is dialysed 24 hours with the bag filter of molecular cut off 3500 in distilled water, obtains carboxylic after purification
Change chitosan/dithiothreitol (DTT)-gold nano cluster.Resulting carboxyl chitosan/dithiothreitol (DTT)-gold nano cluster has strong
Fluorescence, maximum excitation and launch wavelength are respectively that 285 nm and 645 nm(are shown in Fig. 1).
Embodiment 2:
Gold nano cluster solution made from 0.1 mL embodiment 1 is taken to be added to phosphate of 1.9 mL containing 5 μm of ol/L adriamycins slow
In fliud flushing (20 mmol/L, pH=6.0), it is uniformly mixed.As shown in Figure 2, after adriamycin being added, hair of the system in 645 nm
It penetrates light intensity value and obviously lowers (excitation wavelength is 285 nm).
Embodiment 3:
Gold nano cluster solution made from 0.1 mL embodiment 1 is taken to be added to phosphate of 1.9 mL containing 5 μm of ol/L adriamycins slow
In fliud flushing (20 mmol/L, pH=6.0), it is uniformly mixed.One group of blank control group without adriamycin of setting simultaneously is (in Fig. 3
A).It is placed under ultraviolet lamp after reaction and observes color change.As shown in figure 3, after adriamycin is added, the red of gold nano cluster
Fluorescence is almost quenched (B in Fig. 3) completely.
Embodiment 4:
Gold nano cluster solution made from 0.1 mL embodiment 1 is taken to be added to phosphate of 1.9 mL containing 5 μm of ol/L adriamycins slow
In fliud flushing (20 mmol/L, pH=6.0), reacts 0.5 ~ 3 minute after mixing, measure its fluorescence intensity level at 645 nm
F(excitation wavelength is 285 nm).As shown in Figure 4, after reacting 0.5 minute, fluorescence intensity ratio F0/ F variation tends to be steady.
Embodiment 5:
Gold nano cluster solution made from 0.1 mL embodiment 1 is taken to be added to phosphate of 1.9 mL containing 5 μm of ol/L adriamycins slow
In fliud flushing (20 mmol/L, pH=5.0 ~ 11.0), it is uniformly mixed, measures its fluorescence intensity level F(excitation wavelength at 645 nm
For 285 nm).As shown in Figure 5, when the pH value of reaction system is 6.0, fluorescence intensity ratio F0/ F variation reaches maximum.
Embodiment 5:
The phosphate for taking gold nano cluster solution made from 0.1 mL embodiment 1 to be added to 1.9 mL adriamycins containing various concentration is slow
In fliud flushing (20 mmol/L, pH=6.0), it is uniformly mixed.Measuring its fluorescence emission spectrogram of compound after reaction, (excitation wavelength is
285 nm).As shown in fig. 6, the fluorescence of gold nano cluster is gradually suppressed with the increase of doxorubicin concentration.
Embodiment 6:
The phosphate for taking gold nano cluster solution made from 0.1 mL embodiment 1 to be added to 1.9 mL adriamycins containing various concentration is slow
In fliud flushing (20 mmol/L, pH=6.0), it is uniformly mixed.Its fluorescence intensity level F(at 645 nm is measured after reaction to swash
Hair wavelength is 285 nm).According to F0(blank) and F(contain adriamycin) ratio (F0/ F) draw standard curve quantified.Such as
Shown in Fig. 7, adriamycin setting-out line range is 0.05 ~ 2 μm of ol/L, and lowest detection is limited to 0.005 μm of ol/L.
Embodiment 7:
Gold nano cluster solution made from 0.1 mL embodiment 1 is taken to be added to 1.9 mL objects containing disturbance (being 5 μm of ol/L)
Phosphate buffer (20 mmol/L, pH=6.0) in, be uniformly mixed.Its fluorescence at 645 nm is measured after reaction
Intensity value F(excitation wavelength is 285 nm).As shown in figure 8, compared with the fluorescence quenching that adriamycin generates, other chaff interferents
Influence it is negligible.
Embodiment 8:
It takes gold nano cluster solution made from 0.1 mL embodiment 1 to be added in 1.9 mL human serum sample's solution, is uniformly mixed,
Measuring its fluorescence intensity level F(excitation wavelength at 645 nm after reaction is 285 nm).6 standard is bent in conjunction with the embodiments
The content of line computation adriamycin, the recovery of standard addition of human serum sample 95.7 ~ 102.5%, relative standard deviation is 1.12 ~
1.62%。
Claims (5)
1. it is a kind of based on carboxyl chitosan/dithiothreitol (DTT)-gold nano cluster doxorubicin fluorescence detection method, it is characterized in that sharp
Strong photo induced electron transfer occurs after specificity interaction with gold nano cluster and adriamycin, leads to the glimmering of gold nano cluster
Light quenches, to realize the measurement of adriamycin；The gold nano cluster is made of following steps: being by 1 mL concentration
0.4 mol/L sodium hydroxide solution and 1.6 mL concentration are that 20 mg/mL gold chlorides are pre-mixed, and 7.4 mL concentration are then added
The dithiothreitol (DTT) solution for being 0.1 mol/L for 50 mg/mL carboxyl chitosan solution and 10 mL concentration, shakes up and is placed on 37 DEG C
Obtain carboxyl chitosan/dithiothreitol (DTT)-gold nano cluster within isothermal reaction 8 hours in water-bath；By the gold nano group after reaction
Cluster is dialysed 24 hours with the bag filter of molecular cut off 3500 in distilled water, obtains carboxyl chitosan/bis- sulphur Soviet Union after purification
Sugar alcohol-gold nano cluster.
2. according to claim 1 a kind of based on carboxyl chitosan/dithiothreitol (DTT)-gold nano cluster doxorubicin fluorescence
Detection method, it is characterized in that can judge adriamycin according to the fluorescence intensity at 645 nm of maximum emission wavelength of fluorescence spectrum
Concentration.
3. according to claim 1 or 2 a kind of based on carboxyl chitosan/dithiothreitol (DTT)-gold nano cluster adriamycin
The determination step of fluorescence detection method, feature adriamycin is as follows: taking 0.1 mL carboxyl chitosan/dithiothreitol (DTT)-gold nano
The concentration that cluster solution is added to 1.9 mL adriamycins containing various concentration is 20 mmol/L, in the phosphate buffer of pH=6.0,
It is uniformly mixed；The fluorescence intensity level F that its excitation wavelength at 645 nm is 285 nm is measured after reaction；It is glimmering according to blank
Light intensity value F0With the ratio (F of the fluorescence intensity F containing adriamycin0/ F) draw standard curve quantified；Adriamycin measurement
The range of linearity is 0.05 ~ 2 μm of ol/L, and lowest detection is limited to 0.005 μm of ol/L.
4. a kind of method that adriamycin in blood serum sample is quickly measured based on carboxyl chitosan/dithiothreitol (DTT)-gold nano cluster,
It is characterized in that including the following steps: that 0.1 mL carboxyl chitosan/dithiothreitol (DTT)-gold nano cluster solution is taken to be added to 1.9 mL
It in human serum sample's solution, is uniformly mixed, measures the fluorescence that its excitation wavelength at 645 nm is 285 nm after reaction
Intensity value F；The standard curve measured in conjunction with adriamycin calculates the content of adriamycin, and the recovery of standard addition of human serum sample is 95.7
~ 102.5%, relative standard deviation is 1.12 ~ 1.62%.
5. a kind of carboxyl chitosan/dithiothreitol (DTT)-gold nano cluster that is based on according to claim 4 quickly measures serum
The method of adriamycin in sample, it is characterized in that the gold nano cluster is made of following steps: being 0.4 by 1 mL concentration
Mol/L sodium hydroxide solution and 1.6 mL concentration are that 20 mg/mL gold chlorides are pre-mixed, and it is 50 that 7.4 mL concentration, which are then added,
The dithiothreitol (DTT) solution that mg/mL carboxyl chitosan solution and 10 mL concentration are 0.1 mol/L, shakes up and is placed on 37 DEG C of water-baths
Carboxyl chitosan/dithiothreitol (DTT)-gold nano cluster is obtained within isothermal reaction 8 hours in pot；Gold nano cluster after reaction is used
The bag filter of molecular cut off 3500 is dialysed 24 hours in distilled water, obtains carboxyl chitosan/dithiothreitol (DTT)-after purification
Gold nano cluster.
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Application publication date: 20190614 |