CN109874943A - A kind of Chinese yew is preparing the application in the feed for improving chicken meat quality - Google Patents
A kind of Chinese yew is preparing the application in the feed for improving chicken meat quality Download PDFInfo
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- CN109874943A CN109874943A CN201910221106.7A CN201910221106A CN109874943A CN 109874943 A CN109874943 A CN 109874943A CN 201910221106 A CN201910221106 A CN 201910221106A CN 109874943 A CN109874943 A CN 109874943A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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Abstract
The invention discloses a kind of Chinese yew to prepare the application in the feed for improving chicken meat quality, is related to agricultural technology field.The Chinese yew is 2%-4% in the addition weight percent of basal diet as additive；It is preferred that 4%.The raising chicken meat quality, which refers to, to be improved chicken delicate flavour, reduces in chicken and be deposited with taxol in cholesterol level and chicken and liver.Present invention firstly discovers that being used in fairy house chicken feed be remarkably improved fairy house chicken comprehensive nutrient value for Taxus leaf dry powder as additive.
Description
Technical field
The present invention relates to agricultural technology fields more particularly to a kind of Chinese yew to prepare the feed for improving chicken meat quality
In application.
Background technique
With the development of fast food, China's chicken market demand increases, due to the improvement of people's living standards, for chicken
The requirement of meat safety, quality, flavor etc. is also continuously improved, Chinese herbal medicine because its toxic side effect is small, noresidue due to by as antibiosis
Plain substitute is widely used in Feed Manufacturing, and the research for being used to improve chicken meat quality as feed addictive has become poultry
The hot spot of animal husbandry research.The discovery fruit of glossy privet extract such as Wang Huanyu can significantly reduce chicken drip loss, alleviate under chicken pH value
Drop improves chicken tenderness, increases flavor substance inosinicacid in the content of chest muscle.Chen Guoshuns etc. are studies have shown that feeding Chinese herbal medicine adds
Add the broiler chicken of agent, chicken comprehensive score highest, tenderness, mouthfeel, succulence and soup taste are in optimal level.
Taxus (Taxus Linn.) plant belongs to taxaceae (Taxac-eae), usually aiphyllium or filling
Wood is gained the name due to meat aril takes on a red color when seed maturation, referred to as " living fossil " of plant kingdom.It is planted at present from Taxus
Bearing taxanes about more than 500 and a large amount of non-bearing taxanes ingredients, including sesquiterpenoids are isolated in object
Close object, steroid compound, lignin compound, flavone compound, glucoside compound and other class compounds etc..
Scientists have carried out a large amount of to Bakating III class taxane substance in bearing taxanes studies have shown that taxol is to more
Kind advanced cancer curative effect is prominent.The one kind of Chinese yew as Chinese herbal medicine, applied to Production of Livestock and Poultry in terms of research it is very few.
Summary of the invention
In view of this, the embodiment of the invention provides a kind of Chinese yew in preparing the feed for improving chicken meat quality
Using., main purpose is that Chinese yew dry powder is added in egg feedstuff, improves chicken meat quality and nutritive value.
In order to achieve the above objectives, invention broadly provides following technical solutions:
On the one hand, the embodiment of the invention provides a kind of Chinese yew to prepare answering in the feed for improving chicken meat quality
With.
Preferably, the Taxus leaf dry powder is 2%- in the addition weight percent of basal diet as additive
4%.
Preferably, the Taxus leaf dry powder is 4% in the addition weight percent of basal diet as additive.
It improves chicken delicate flavour preferably, the raising chicken meat quality refers to, reduce cholesterol level and chicken in chicken
Taxol is deposited in meat and liver.
On the other hand, the embodiment of the invention provides a kind of fairy house chicken feed addictive, the additive is Taxus leaf
Dry powder.
In another aspect, the feed is by basal diet and additive the embodiment of the invention provides a kind of fairy house chicken feed
Composition；The additive is above-mentioned fairy house chicken feed addictive；Addition of the additive in the basal diet, which is compared, is
2%-4%, most suitable adding proportion are 4%.
Preferably, the formula of the basal diet are as follows: corn 54%, rice bran 5%, dregs of beans 26%, fish meal 2%, shell
3%, calcium phosphate 6.5%, di(2-ethylhexyl)phosphate light calcium 1.2%, salt 0.3%, additive 2%.
Compared with prior art, the beneficial effects of the present invention are:
Chinese yew (taxol) is applied in chicken feed by the present invention as feed addictive, by system research in daily ration
Shadow of the Chinese yew dry powder (taxol) of middle addition different level to Xianju Chicken chicken inosinicacid, intramuscular fat and cholesterol level
The changing rule of deposition of the loud and taxol in chicken and liver finds Chinese yew as additive in basal diet
Addition content can improve the content of inosinicacid, intramuscular fat when being 2%-4% (preferably 4%), improve chicken delicate flavour and reduce chest
The content of cholesterol in flesh, and taxol can be deposited in chicken, improve the synthesis edible value of chicken, be Chinese yew
The application of (taxol) in production provides scientific basis.
Specific embodiment
For further illustrate the present invention to reach the technical means and efficacy that predetermined goal of the invention is taken, below with compared with
Good embodiment, to specific embodiment, technical solution, feature and its effect applied according to the present invention, detailed description is as follows.Under
Stating the special characteristic, structure or feature in multiple embodiments in bright can be combined by any suitable form.
Embodiment 1
Chinese yew: test is the prior year from the branches and leaves under Chinese yew trimming with Chinese yew dry powder, after natural lighting is dried
It smashes, content of taxol is 19.6 μ g/g, is provided by Wuyi In Zhejiang Taxus x media developmental research Co., Ltd.
140 ages in days are chosen in test, and the Xianju Chicken being in a good state of health 400 is randomly divided into 4 groups, every group of 5 repetitions, often
A repetition 20.A group is control group, feeds basal diet, and B, C and D group are test group, is added respectively on the basis of Basic drawing
Add 2%, 3% and 4% Chinese yew dry powder.Basal diet is provided by Zhejiang Province's fairy house kind chicken house, and daily ration composition and trophic level are shown in
Table 1.For test chicken only with double-deck stereo cage, henhouse is semi-enclosed, natural lighting and ventilation, experimental period 90d.Entire test
Mash feed, free water is freely eaten in period, chicken, and test process records the health condition of each group chicken in detail, just by chicken house
Normal immune programme is immunized and is sterilized, and feeding and management condition is consistent.
1. basal diet of table composition and nutritional ingredient
Note: contain V in every kilogram of feedA1500IU, VD3200IU, VE10IU, VB23.5mg, VB310mg, niacin
30mg, VB1210 μ g, choline chloride 1 000mg, VH0.15mg, VB0.5mg, VB11.5mg, VB63.0mg, iron 80mg, zinc
40mg, Mn 60mg, iodine 0.18mg, Cu 8mg, Se 0.3mg.
Collect sample: in 60d, 90d, each repetition 1 of every group of A, B, C, D group Xianju Chicken, butchers removing chest by totally 20
Flesh, leg flesh, liver, are loaded on hermetic bag, and -80 DEG C of refrigerators freeze standby survey.
Test index and method:
(1) content of taxol, using clarithromycin as internal standard, blood the detection of content of taxol: are measured referring to the methods of Chen Hua
Clearly, liver, chest muscle homogenate carry out the measurement of HPLC-MS method respectively after acetonitrile precipitation albumen.
(2) detection of inosine acid content:
Sample preparation: 1.25g frost meat sample is weighed, is put into culture dish, is shredded with scissors, is placed in homogenate tube, add 4mL
It is homogenized after 6% perchloric acid, extract is gone into 10mL centrifuge tube, washed homogenate tube with 6% perchloric acid of 1mL, pour into centrifuge tube
In, 3500rmin-1 is centrifuged 5min, and supernatant is poured into 50mL beaker, and precipitating vibrates 5min with 6% perchloric acid of 2mL again
After be centrifuged, supernatant merges with previous, respectively with 5,0.5molL-1 sodium hydroxide tune pH to 6.5, go to 25mL volumetric flask,
It is settled to scale with distilled water, is shaken up, 0.45 μm of membrane filtration, filtrate is used for efficient liquid phase chromatographic analysis.
Chromatographic condition: 1. instrument: Waters high performance liquid chromatograph, including 510 pumps, 486 UV detector, U6K sample introduction
Device；2. chromatographic column: 18 radially compressed column of Waters C (10 μm, 8 × 100mm)；3. mobile phase: 0.05molL-1 triethylamine
(with phosphoric acid tune pH to 6.5) and methanol (volume ratio 95: 5) liquid；4. flow velocity: 0.5mLmin-1.
(3) detection of intramuscular fat content:
Sample preparation: frozen samples through thaw at RT, removing muscle surface visible fat, between sarolemma and sarolemma after fat,
It is shredded using blade, then is rubbed through meat grinder, sample to be tested is made.
Measuring method: taking 4g or so sample, is placed in 2 added in the filter paper after 10g yellow ground and drying weighing, drying is made
At absolute dried sample (it is put into sample at 65 DEG C and is warming up to after 103 DEG C and starts timing, dries 2h), measures over dry moisture.After drying weighing
Filter paper packet (contain sample) be put into Soxhlet extractor, by GB/T 5009.6-2003 " fatty measurement in food " (Soxhlet pumping
Follow the example of) operating procedure, it the processing such as is dried, extracts, measuring fat content.Fat content (%)=(extract preceding paper bag weight-
Paper bag weight after extraction)/(paper bag weight-empty paper bag weight before extracting) × 100.
(4) detection of cholesterol level:
Sample preparation: by the chicken slaughter after blood sampling, chest muscle, leg flesh, liver are taken out immediately, 0.5g is weighed, its quality is added
After the physiological saline homogenate of 9 times of volumes of number, it is sub-packed in Eppendorf pipe, sample is put into self-sealing plastic bag, sets 70 DEG C of guarantors
It deposits, it is standby to survey.
Measuring method: 3 000g of homogenate is centrifuged 10min, takes supernatant 2ml to be dried up in 65 DEG C of water-baths with N2, in residue
It is added 10ml KOH- alcoholic solution (90% ethyl alcohol Fresh of 12%KOH), is saponified 15min in 80 DEG C of water-baths.It has been saponified
Finish, takes out test tube, add 5ml distilled water, be cooled to room temperature.Non-saponified portions is extracted with 10ml hexane, take after stratification 5ml oneself
Alkane extracting solution is dried up in 65 DEG C of water-baths with N 2.It is being produced daily with the Cholesterol Kit that Ningbo Ci Cheng biochemical reagents factory provides
It is measured on 5 type semi-automatic biochemical analyzer of CHEM-.
Data analysis: all test datas are arranged using Excel 2007, using 17.0 software of SPSS to arrangement number
According to progress variance analysis, and using significance test of difference between LSD and Duncan ' s method of inspection progress each group, P < 0.05 is difference
Significantly；Test data is indicated with " mean+SD ".
As a result with analysis:
(1) deposition of the taxol in chest muscle, leg flesh, liver: addition different level Chinese yew is to fairy house thoracic muscle, leg
The influence of content of taxol is shown in Table 2 in flesh, liver.As can be seen from Table 2, the content of taxol is not detected in A group in experimental period 60d,
B, C, D group can detecte the content of taxol in chest muscle, leg flesh, liver, but content of taxol difference is not between each group
Significantly (P > 0.05)；Content of taxol is not detected in experimental period 90d, A group chest muscle, content of taxol is aobvious in C, D group chest muscle
It writes and is higher than B group (P<0.05), C, D group difference is not significant (P>0.05), and content of taxol B, C, D group difference is not shown in leg flesh
Write (P>0.05), content of taxol C, D group is significantly higher than A group (P<0.05) in liver, C, D group difference it is not significant (P>
0.05)。
(2) influence of the Chinese yew pitch-based sphere to chest muscle inosinicacid, intramuscular fat, cholesterol level: seen from table 3: with A
Group is compared, and D group chest muscle inosinicacid is higher than A group (P<0.05), and A, B, C group difference is not significant (P>0.05), and B, D group difference are not
Significantly (P>0.05), B, C group difference is not significant (P>0.05), and C, D group difference are significant (P<0.05)；Chest muscle intramuscular fat C,
D group is significantly higher than A group (P<0.05), and C, D group difference is not significant (P>0.05), and B, C group difference are significant (P<0.05), B, D
Group difference is not significant (P>0.05), and A, B group difference is not significant (P>0.05), chest muscle cholesterol D group be lower than A group (P<
0.05), A, B, C group difference is not significant (P > 0.05), and B, C, D group difference is not significant (P > 0.05).
(3) influence of the Chinese yew pitch-based sphere to leg flesh inosinicacid, intramuscular fat, cholesterol level: from table 4: with A
Group is compared, and leg flesh inosinicacid B, C, D group difference is not significant (P > 0.05)；Leg flesh intramuscular fat B group substantially less than A group (P <
0.05), B, C, D group difference is not significant (P > 0.05), and A, C, D group difference is not significant (P > 0.05)；Between cholesterol B, C, D group
Difference is not significant (P > 0.05).
(4) influence of the Chinese yew pitch-based sphere to liver intramuscular fat, cholesterol level: from table 5: compared with A group,
B, fat and cholesterol level difference is not significant (P > 0.05) in C, D group liver.
Influence of the 2. Chinese yew pitch-based sphere of table to chest muscle, leg flesh, liver content of taxol
Influence of the 3. Chinese yew pitch-based sphere of table to chest muscle inosinicacid, intramuscular fat, cholesterol level
Influence of the 4. Chinese yew pitch-based sphere of table to leg flesh inosinicacid, intramuscular fat, cholesterol level
Influence of the 5. Chinese yew pitch-based sphere of table to liver intramuscular fat, cholesterol level
Pass through the relationship of above-mentioned each analysis of experimental data taxol and chicken nutritional ingredient:
(1) deposition of the taxol in chest muscle, leg flesh, liver: taxol (Paclitaxel, trade name Taxol) is
A kind of diterpene-kind compound.Some researches show that taxols in addition to having antimitotic effect, still there is activating macrophage mediation
Antitumor action.The antitumaous effect mechanism of taxol is mainly the mitosis end for passing through and microtubule depolymerization being inhibited to make tumour cell
Only and make apoptosis of tumor cells, finally results in death of neoplastic cells.
The present invention tests discovery, and Xianju Chicken is searched for food after Chinese yew, and the taxol in Chinese yew can be transferred to chicken from diet
In meat, illustrating can be using chicken as the carrier of taxol；But the content of taxol in chicken is lower, may be due in Chinese yew
Taxol is most of through liver metabolism, only deposits in chicken on a small quantity.
(2) influence of the Chinese yew pitch-based sphere to inosine acid content: as the improvement of people's living standards, delicious meat,
The meat of unique flavor is gradually by the favor of consumer, however, due to the continuous improvement of breeding performonce fo animals, flavor quality
Show the trend gradually decreased.Inosinicacid (inosinic acid, IMP) is the main matter for determining animal meat quality delicate flavour,
As the important indicator of meat analysis, it is widely used in the flavor evaluation of various animal meat products, content height is indirect
Reflect the superiority and inferiority of meat product flavor.
The Chinese yew discovery of different level is added in present invention experiment in Xianju Chicken diet, and the content of chest muscle inosinicacid is higher than
Leg flesh (P < 0.05), and D group inosine acid content is higher than A group in chest muscle, illustrates that Chinese yew is added in diet can improve flesh
The content of thuja acid.
(3) influence of the Chinese yew pitch-based sphere to intramuscular fat content: animal-based protein is that the main albumen of the mankind comes
Source, animal muscle tissue are wherein most important a part；One of an important factor for being influence Quality of poultry meatan issue of growing importance, appropriate intramuscular rouge
Fat content can improve the tenderness and flavor of muscle.It is found that with the increase of IMF content, muscle fibre between 1%~3%
Can be thinner, and the connective tissue that connection is formed between muscle fibre is also fewer, the shearing force of muscle is lower, and the tenderness of meat is better.IMF content
At 2%~3%, mouthfeel is best, and muscle dries out when being lower than 2%, and entrance sends out bavin；Since fat content is excessively high when higher than 3%, enter
Mouth is felt sick of, while can not cater to demand of the people to low-fat diet.
The present invention test discovery addition different level Chinese yew after, the intramuscular fat in chest muscle between 2.5%~3%,
Intramuscular fat content is 2.0%~2.5% in leg flesh.Although belonging to white adipose, IMF and subcutaneous fat and interior fat
Metabolism have differences, the deposition rate of IMF is also slower compared with subcutaneous fat and interior fat.
(4) influence of the Chinese yew pitch-based sphere to cholesterol level: cholesterol be human body maintain normal physiological activity must
Need substance.But the cholesterol of human body excess intake can be such that Blood Cholesterol concentration increases, and cause atherosclerosis, induce
A series of cardiovascular and cerebrovascular diseases such as coronary heart disease, hypertension.Chicken is one of favorite animal foodstuff of the mankind, reduce chicken and its
Cholesterol level in linked groups seems particularly necessary to chicken quality.
Present invention experiment finds that the gallbladder in fairy house thoracic muscle is solid when adding the Chinese yew of different level in Xianju Chicken diet
Alcohol content has dropped；This may be related with Flavonoid substances are contained in Chinese yew.
The present invention has found that the Chinese yew that different level is added in laying hen diets can improve by above-mentioned experimentation
Chicken meat quality, the content for reducing cholesterol, while the taxol in Chinese yew can deposit to chicken, in liver, in liver
Taxol deposition highest；It is recommended that optimum addition is 4%.
Place, those skilled in the art can not select from the prior art to the greatest extent in the embodiment of the present invention.
Disclosed above is only a specific embodiment of the invention, but scope of protection of the present invention is not limited thereto, is appointed
What those familiar with the art in the technical scope disclosed by the present invention, can easily think of the change or the replacement, answer
It is included within the scope of the present invention.Therefore, protection scope of the present invention should be with above-mentioned scope of protection of the claims
It is quasi-.
Claims (4)
1. a kind of Chinese yew is preparing the application in the feed for improving chicken meat quality.
2. a kind of Chinese yew as described in claim 1 is preparing the application in the feed for improving chicken meat quality, feature
It is, the Chinese yew is 2%-4% in the addition weight percent of basal diet as additive.
3. a kind of Chinese yew as claimed in claim 2 is preparing the application in the feed for improving chicken meat quality, feature
It is, the Chinese yew is 4% in the addition weight percent of basal diet as additive.
4. a kind of taxol as described in claim 1 is preparing the application in the feed for improving chicken nutritional ingredient,
It is characterized in that, the raising chicken meat quality, which refers to, to be improved chicken delicate flavour, reduces cholesterol level and chicken and liver in chicken
In be deposited with taxol.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107348165A (en) * | 2017-09-11 | 2017-11-17 | 聂淑琼 | A kind of egg feedstuff and preparation method thereof |
-
2019
- 2019-03-22 CN CN201910221106.7A patent/CN109874943A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107348165A (en) * | 2017-09-11 | 2017-11-17 | 聂淑琼 | A kind of egg feedstuff and preparation method thereof |
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Application publication date: 20190614 |
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