CN109868289A - The resource utilization method of wheat vinasse - Google Patents
The resource utilization method of wheat vinasse Download PDFInfo
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- CN109868289A CN109868289A CN201910225973.8A CN201910225973A CN109868289A CN 109868289 A CN109868289 A CN 109868289A CN 201910225973 A CN201910225973 A CN 201910225973A CN 109868289 A CN109868289 A CN 109868289A
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Abstract
The present invention discloses a kind of resource utilization method of wheat vinasse, and the resource utilization methods of the wheat vinasse after distillation extracts ethyl alcohol, obtains wheat vinasse stoste the following steps are included: carry out saccharification enzymatic hydrolysis by wheat flour and saccharomyces cerevisiae is added to ferment；The first enzyme preparation is added into wheat vinasse stoste, carries out the toxin of enzymatic hydrolysis degradation for the first time, to remove the biotoxin in the wheat vinasse stoste, obtains enzymatic hydrolysis vinasse.Technical solution of the present invention can effectively remove biotoxin therein, so that following resourceization reaches state health standards using obtained byproduct.
Description
Technical field
The present invention relates to wheat vinasse technical field, in particular to a kind of resource utilization method of wheat vinasse.
Background technique
Wheat vinasse are usually the byproduct that fermentative production of ethanol obtains, and main component is polysaccharide, albumen and organic acid
Deng.In order to sufficiently recycle resource to avoid the wasting of resources, people would generally carry out deep resource utilization to wheat vinasse
To obtain various byproducts.But it can if be not removed to it due to containing a large amount of biotoxin in wheat vinasse
So that finally obtained byproduct cannot reach state health standards because of due to containing biotoxin, needing to be further processed can just make
With.
Above content is only used to facilitate the understanding of the technical scheme, and is not represented and is recognized that above content is existing skill
Art.
Summary of the invention
The main object of the present invention is to provide a kind of resource utilization method of wheat vinasse, it is intended to effectively remove therein
Biotoxin, so that following resourceization reaches state health standards using obtained byproduct.
To achieve the above object, the resource utilization method of wheat vinasse proposed by the present invention, comprising the following steps:
The wheat fecula that gives up is fermented, after distillation extracts ethyl alcohol, obtains wheat vinasse stoste；
The first enzyme preparation is added into wheat vinasse stoste, the toxin of enzymatic hydrolysis degradation for the first time is carried out, to remove the wheat
Biotoxin in vinasse stoste obtains enzymatic hydrolysis vinasse.
Optionally, the additional amount of first enzyme preparation is the 0.5%-3% of the wheat vinasse stock solution quality.
Optionally, first enzyme preparation is the bacterium enzyme mix preparation of bacillus and vomitoxin detoxification enzyme, aspergillus flavus
Plain catabolic enzyme and facultative aerobic character Bifidobacterium.
Optionally, bacterium enzyme mix preparation, the aflatoxins catabolic enzyme of the bacillus and vomitoxin detoxification enzyme
And the mass ratio of the facultative aerobic character Bifidobacterium is (3-6): (2-5): (0.5-2).
Optionally, the pH value of the first time enzymatic hydrolysis degradation toxin is 3-7.5, and hydrolysis temperature is 20 DEG C -50 DEG C, and enzymatic hydrolysis is anti-
It is 2h-8h between seasonable.
Optionally, " the first enzyme preparation is being added in Xiang little Mai vinasse stoste, the toxin of enzymatic hydrolysis degradation for the first time is being carried out, to remove
Remove the biotoxin in the wheat vinasse stoste, obtain enzymatic hydrolysis vinasse " the step of after further include:
The second enzyme preparation is added, carries out second and digests, to obtain secondary enzymolysis vinasse.
Optionally, the additional amount of second enzyme preparation is the 0.1%-0.5% of the wheat vinasse stock solution quality.
Optionally, second enzyme preparation is cellulase, xylo-oligosaccharide enzyme, protease and wall breaking enzyme.
Optionally, the mass ratio of the cellulase, the xylo-oligosaccharide enzyme, the protease and the wall breaking enzyme is
(1-5):(10-30):(20-50):(1-10)。
Optionally, the pH value of second enzymatic hydrolysis is 3-7.5, and hydrolysis temperature is 50 DEG C -75 DEG C, and the enzyme digestion reaction time is
8h-12h。
Technical solution of the present invention, by the way that the first enzyme preparation is added in wheat vinasse, to effectively remove in wheat vinasse
A large amount of biotoxins so that in the subsequent byproduct that wheat vinasse are goed deep into working process containing it is less amount of biology poison
Element can reach state health standards, not need to be further processed, and can directly use.
Detailed description of the invention
In order to more clearly explain the embodiment of the invention or the technical proposal in the existing technology, to embodiment or will show below
There is attached drawing needed in technical description to be briefly described, it should be apparent that, the accompanying drawings in the following description is only this
Some embodiments of invention for those of ordinary skill in the art without creative efforts, can be with
The structure shown according to these attached drawings obtains other attached drawings.
Fig. 1 is the process flow chart for one embodiment of method that wheat wine residue resource of the present invention utilizes.
The embodiments will be further described with reference to the accompanying drawings for the realization, the function and the advantages of the object of the present invention.
Specific embodiment
The technical scheme in the embodiments of the invention will be clearly and completely described below, it is clear that described implementation
Example is only a part of the embodiments of the present invention, instead of all the embodiments.Based on the embodiments of the present invention, this field is general
Logical technical staff every other embodiment obtained without creative efforts belongs to what the present invention protected
Range.
It in addition, the technical solution between each embodiment can be combined with each other, but must be with ordinary skill
Based on personnel can be realized, this technical side will be understood that when the combination of technical solution appearance is conflicting or cannot achieve
The combination of case is not present, also not the present invention claims protection scope within.
The present invention proposes a kind of resource utilization method of wheat vinasse, the resource utilization method the following steps are included:
Step 1 ferments the wheat fecula that gives up, and after distillation extracts ethyl alcohol, obtains wheat vinasse.
Specifically, by remaining after using dry process to obtain wheat protein powder (gluten) and wheaten starch wheat grain
Useless fecula, and to useless fecula carry out saccharification enzymatic hydrolysis and be added saccharomyces cerevisiae carry out fermentation process, it is right after being fermented into ethyl alcohol
Ethyl alcohol carries out distillation extraction, and remaining fermentation dope is then wheat vinasse stoste, and solid content is in the wheat vinasse stoste
8%-20%.The reasonable resourceization that the step can be realized wheat grain utilizes, and efficiently avoids the waste of resource.
The first enzyme preparation is added in Xiang little Mai vinasse stoste for step 2, carries out the toxin of enzymatic hydrolysis degradation for the first time, small to remove
Biotoxin in brewer's grains stoste obtains enzymatic hydrolysis vinasse.
Due to containing a large amount of biotoxin in wheat vinasse, then the first enzyme preparation is added into wheat vinasse, to it
In biotoxin carry out enzymatic removal, obtain containing a small amount of biotoxin or even be free of the wheat vinasse of biotoxin, so
Wheat vinasse are carried out to can achieve country substantially free of biotoxin in the byproduct that recycling deep processing obtains subsequent
Sanitary standard.
Accordingly, it is to be understood that, technical solution of the present invention, by the way that the first enzyme preparation is added in wheat vinasse, to have
A large amount of biotoxins in effect removal wheat vinasse, so that subsequent go deep into the byproduct of working process containing to wheat vinasse
There is less amount of biotoxin, state health standards can be reached, do not need to be further processed, can directly use.
Optionally, the additional amount of the first enzyme preparation is the 0.5%-3% of wheat vinasse stock solution quality.First enzyme preparation adds
Entering amount will be suitable for, so that the first time enzymatic hydrolysis degradation toxin of wheat vinasse is more thorough, to more efficiently remove therein
Biotoxin, such as the additional amount of the first enzyme preparation is the 0.5% or 0.7% or 1% or 2% of wheat vinasse, or
Person 2.5% or 3%.
Since the biotoxin in wheat vinasse is usually vomitoxin and aflatoxin, then needs that the first enzyme is added
Preparation is usually vomitoxin (also known as DON- deoxynivalenol) and aspergillus flavus poison with biotoxin therein of degrading
Element.Optionally, the first enzyme preparation be the bacterium enzyme mix preparation of bacillus and vomitoxin detoxification enzyme, aflatoxins catabolic enzyme and
Facultative aerobic character Bifidobacterium.
The bacterium enzyme mix preparation of bacillus and vomitoxin detoxification enzyme, can be by vomitoxin as a kind of feed addictive
In DON be converted into 3-epi-DON, it is the minimum DON of current toxicity that the toxicity of 3-epi-DON, which reduces by 350 times or more compared with DON,
Metabolite can also efficiently remove vomitoxin therein.Aflatoxins catabolic enzyme and facultative aerobic character Bifidobacterium are total to
Same-action can preferably remove aflatoxin therein.
When preparing the first enzyme preparation, one of the various enzyme preparation additional amounts also will be suitable for, to give full play to various enzymes
Digest degradation.Optionally, the bacterium enzyme mix preparation of bacillus and vomitoxin detoxification enzyme, aflatoxins catabolic enzyme and simultaneous
The mass ratio of oxygen Bifidobacterium is (3-6): (2-5): (0.5-2).
Optionally, the pH value of the toxin of enzymatic hydrolysis degradation for the first time is 3-7.5, and hydrolysis temperature is 20 DEG C -50 DEG C, when enzyme digestion reaction
Between be 2h-8h.
Strict control is had to when the first enzymatic hydrolysis degradation toxin operation and digests operating condition well, to guarantee enzymatic hydrolysis operation more
Add and is effectively performed.For example, when enzymatic hydrolysis pH value control 3 or 5 or 6 or 6.5 or 7 or 7.5, hydrolysis temperature control at 20 DEG C or
30 DEG C or 40 DEG C or 50 DEG C；The enzyme digestion reaction time be 2h or 3h or 4h or 5h or 6h or 7h or
8h.If perhaps hydrolysis temperature is lower than 20 DEG C lower than 3 or the enzyme digestion reaction time is less than 8h for pH value when enzymatic hydrolysis, then it can make enzyme
Solution preocess is not thorough enough, causes finally obtained feed product content of toxins that cannot reach sanitary index.If if pH when enzymatic hydrolysis
Value is higher than 7.5, and perhaps hydrolysis temperature is higher than 50 DEG C or the enzyme digestion reaction time is higher than 8h, then when can make entire enzymolysis process
Between it is longer so that production efficiency is lower.
Further, be added in Xiang little Mai vinasse stoste the first enzyme preparation carry out for the first time enzymatic hydrolysis degradation toxin the step of it
Afterwards further include: the second enzyme preparation is added, carries out second and digests, to obtain enzymatic hydrolysis vinasse.
Second of enzymatic hydrolysis operation mainly so that by wheat vinasse stoste processing byproduct increment obtained, increases final make
The content of the prebiotics such as protein small peptide and xylo-oligosaccharide in the byproduct obtained.The viscosity of wheat vinasse stoste is also reduced simultaneously,
Convenient for the operation of subsequent separation of solid and liquid.
Optionally, the additional amount of the second enzyme preparation is the 0.1%-0.5% of wheat vinasse stock solution quality.Second enzyme preparation
Additional amount will be suitable for, so that second of enzymatic hydrolysis of wheat vinasse is more thorough, to more effectively reduce wheat vinasse stoste
Viscosity improve solid-liquid separation efficiency in order to the operation of subsequent separation of solid and liquid, such as the enzyme total amount that second enzymatic hydrolysis is added is small
The 0.1% of brewer's grains or 0.2% or 0.5%.
Optionally, the second enzyme preparation is cellulase, xylo-oligosaccharide enzyme, protease and wall breaking enzyme.
After cellulase and xylo-oligosaccharide enzyme are added, under its synergistic effect, the crude fibre in vinasse stoste can reduce,
The content for increasing the soluble polysaccharides such as polyxylan and xylo-oligosaccharide in product, moreover, polyxylan therein is in animal digestion
Degradable in system is xylo-oligosaccharide, and for xylo-oligosaccharide as a kind of prebiotics, content is higher, and activity is higher, has been more advantageous to
The growth of beneficial bacteria, so that final products realize increment.Also, the viscosity that can also reduce vinasse stoste, since polysaccharide is relatively easy to
Binding protein easily causes subsequent filters pressing operation to block the filter opening of filter press, therefore reduces the viscosity of vinasse, can have after being convenient for
It is continuous to be separated by solid-liquid separation operation, improve separative efficiency.
The addition of protease can be improved the content of soluble protein in vinasse stoste, while can also improve final be made
Byproduct in wheat protein peptide content so that byproduct realize increment.
The addition of wall breaking enzyme, can the yeast that stays of broken wall fermentation alcohol, sporoderm-broken rate can be improved to 95% or more, increases
Add the content of original yeast extract.Also, it is acted on proteinase synergy, more protein peptides can be extracted.Simultaneously as complete
Whole yeast cells will also result in the increase of vinasse stoste viscosity, can also have after broken wall convenient for the operation of subsequent separation of solid and liquid, improve
Its separative efficiency.
When preparing the second enzyme preparation, wherein the additional amount of various enzyme components also will be suitable for, to give full play to various enzymes
Enzymolysis, so that finally obtained byproduct, which is realized, maximizes increment.Cellulase, xylo-oligosaccharide enzyme, protease and
The mass ratio of wall breaking enzyme is (1-5): (10-30): (20-50): (1-10).
Optionally, the pH value of second of enzymatic hydrolysis is 3-7.5, and hydrolysis temperature is 50 DEG C -75 DEG C, and the enzyme digestion reaction time is 8h-
12h。
Strict control is also required to when digest for second and digests operating condition well, to guarantee that enzymatic hydrolysis operation is more efficient
Ground carries out.For example, 3 or 5 or 6.5 or 7 or 7.5, hydrolysis temperature is controlled at 50 DEG C or 60 DEG C or 75 for pH value control when enzymatic hydrolysis
℃；The enzyme digestion reaction time is 8h perhaps 10h or 12h.If pH value is lower than 3 when enzymatic hydrolysis or hydrolysis temperature is lower than 50 DEG C,
Or the enzyme digestion reaction time is less than 8h, then enzymolysis process can be made not thorough enough, finally obtained feed product toxin is caused to contain
Amount cannot reach sanitary index.If if higher than 7.5, perhaps hydrolysis temperature is higher than 75 DEG C or enzyme digestion reaction to pH value when enzymatic hydrolysis
Time is higher than 12h, then the entire enzymolysis process time can be made longer, so that production efficiency is lower.
It should be noted that the sequence of enzymatic hydrolysis and second of enzymatic hydrolysis cannot exchange for the first time, because most harmful thin
The survival temperature of bacterium is 60 DEG C hereinafter, the operation that needs to cool down if first carrying out second digesting, will cause germ contamination, and pass through
Second of enzymatic hydrolysis is carried out again after enzymatic hydrolysis detoxification for the first time, and temperature is increased to 70 DEG C, and most of harmful bacteria is dead, then
Final residual product contamination is not will cause.
Of course, other also can be selected with identical function or similar function in the enzyme that enzymatic hydrolysis and second of enzymatic hydrolysis are added for the first time
Can enzyme preparation substitute.
Further, the wheat vinasse after digesting twice can be separated by solid-liquid separation, and further respectively to solid slag and clear
Liquid carries out subsequent deep processing, contains less biology in the byproduct that can be obtained different byproducts, and finally obtain
Toxin can reach state health standards, can directly use.
In one embodiment of this invention, referring to Fig. 1, it is different to obtain to wheat vinasse stoste progress subsequent processing
Byproduct, it may be assumed that firstly, the enzymatic hydrolysis vinasse after secondary enzymolysis are carried out filters pressing operation, distiller's grains liquor and vinasse solid slag are obtained, into one
Vinasse solid slag is dried in step, can get dry feedstuff A, and biotoxin content is less in feedstuff A, can be with
Direct nutrition purposes, especially for feeding animals.Distiller's grains liquor is further used into Ultra filtration membrane, obtains ultra-filter retentate and ultrafiltration clear liquid, ultrafiltration is cut
Feedstuff B can be obtained by staying liquid successively to carry out concentration and drying, and the main component of feedstuff B is that albumen and macromolecular are water-soluble
Property polysaccharide byproduct；The isolated nanofiltration retentate fluid of nanofiltration membrane and nanofiltration clear liquid can further be used for ultrafiltration clear liquid, to receiving
Filter trapped fluid is successively concentrated and is dried and can be obtained feed addictive A, can be added in feed and be carried out nutrition purposes, especially for feeding animals.For receiving
Cleaner liquid further can obtain reverse osmosis trapped fluid and counter-infiltration clear liquid by reverse osmosis membrane separation, successively to reverse osmosis trapped fluid
Feed addictive B can be obtained by carrying out concentration and drying, can be added in feed and be carried out nutrition purposes, especially for feeding animals.It, can for counter-infiltration clear liquid
The water for meeting reuse or discharge standard is further obtained by biochemical treatment.In this way, the depth to wheat vinasse stoste can be realized
Change processing to obtain different byproducts, avoids the wasting of resources, realize the effect that recycling rationally utilizes to a certain extent.
Of course, other than the byproduct that above-mentioned in-depth is handled, other pairs can also be obtained by other Deepen processing methods
Product, this is no longer going to repeat them.
It is described in detail below by way of resource utilization method of the specific embodiment to wheat vinasse of the present invention.
Embodiment 1
Step 1, by remaining after using dry process to obtain wheat protein powder (gluten) and wheaten starch wheat grain
Useless fecula, and to wheat flour give up fecula carry out saccharification enzymatic hydrolysis and be added saccharomyces cerevisiae carry out fermentation process, be fermented into second
After alcohol, distillation extraction is carried out to ethyl alcohol, remaining fermentation dope is then wheat vinasse stoste.
Step 2, is added the first enzyme preparation that mass fraction is 0.5% in Xiang little Mai vinasse stoste, and in pH value be 7.5,
Hydrolysis temperature is 20 DEG C, the toxin of enzymatic hydrolysis degradation for the first time is carried out under the operating condition that the enzyme digestion reaction time is 8h, to remove wheat
Biotoxin in vinasse stoste.Wherein, the first enzyme preparation be bacillus and vomitoxin detoxification enzyme bacterium enzyme mix preparation,
Aflatoxins catabolic enzyme and facultative aerobic character Bifidobacterium, and bacterium enzyme mix preparation, the Huang Qu of bacillus and vomitoxin detoxification enzyme
Mycin catabolic enzyme and the mass ratio of facultative aerobic character Bifidobacterium are 6:5:2.
Step 3, be subsequently added into mass fraction be 0.1% the second enzyme preparation, and in pH value be 7.5, hydrolysis temperature 50
DEG C, second is carried out under the operating condition that the enzyme digestion reaction time is 8h and is digested, to obtain enzymatic hydrolysis vinasse, wherein the second enzyme preparation is
Cellulase, xylo-oligosaccharide enzyme, protease and wall breaking enzyme, and the quality of cellulase, xylo-oligosaccharide enzyme, protease and wall breaking enzyme
Than for 1:10:20:1.
It should be noted that digesting degradation toxin front and back to first time during utilizing to wheat wine residue resource
Vinasse stoste in the content of vomitoxin and aflatoxin detected, testing result is shown in Table 1.
Table 1 digests the content detection knot of vomitoxin and aflatoxin in the vinasse stoste before and after degrading toxin for the first time
Fruit
Illustrate: detection method is the detection method in national examination criteria, similarly hereinafter.
As can be seen from Table 1, after first time digests degradation toxin, vomitoxin and aflatoxin can be effective
Ground removal, and content meet vomitoxin content in feedstuff specified in national forage health standard GB13078-2017≤
5mg/Kg (5000 μ g/kg), aflatoxin minimum require to be≤10 μ g/kg.
Similarly, also the viscosity of the vinasse stoste before and after second of enzymatic hydrolysis degradation toxin is detected, detection knot
Fruit is shown in Table 2.
The viscosity measurements result of the vinasse stoste of second of table 2 enzymatic hydrolysis front and back
As can be seen from Table 2, after the second enzymolysis processing, the viscosity of wheat vinasse stoste is substantially reduced, and is had after being convenient for
It is continuous to be separated by solid-liquid separation operation, its separative efficiency is improved, to improve the yield of final byproduct.
Embodiment 2
Step 1, by remaining after using dry process to obtain wheat protein powder (gluten) and wheaten starch wheat grain
Useless fecula, and to wheat flour give up fecula carry out saccharification enzymatic hydrolysis and be added saccharomyces cerevisiae carry out fermentation process, be fermented into second
After alcohol, distillation extraction is carried out to ethyl alcohol, remaining fermentation dope is then wheat vinasse stoste.
Step 2, is added the first enzyme preparation that mass fraction is 0.6% in Xiang little Mai vinasse stoste, and in pH value be 3, enzyme
Solving temperature is 50 DEG C, the toxin of enzymatic hydrolysis degradation for the first time is carried out under the operating condition that the enzyme digestion reaction time is 2h, to remove wheat wine
Biotoxin in poor stoste.Wherein, the first enzyme preparation is bacterium enzyme mix preparation, the Huang of bacillus and vomitoxin detoxification enzyme
Aspergillin catabolic enzyme and facultative aerobic character Bifidobacterium, and the bacterium enzyme mix preparation of bacillus and vomitoxin detoxification enzyme, aspergillus flavus
Plain catabolic enzyme and the mass ratio of facultative aerobic character Bifidobacterium are 3:2:0.5.
Step 3, be subsequently added into mass fraction be 0.5% the second enzyme preparation, and in pH value be 7.5, hydrolysis temperature 75
DEG C, second is carried out under the operating condition that the enzyme digestion reaction time is 12h and is digested, to obtain enzymatic hydrolysis vinasse, wherein the second enzyme preparation
For cellulase, xylo-oligosaccharide enzyme, protease and wall breaking enzyme, and the matter of cellulase, xylo-oligosaccharide enzyme, protease and wall breaking enzyme
Amount is than being 5:20:30:10.
It should be noted that digesting degradation toxin front and back to first time during utilizing to wheat wine residue resource
Vinasse stoste in the content of vomitoxin and aflatoxin detected, testing result is shown in Table 3.
Table 3 digests the content detection knot of vomitoxin and aflatoxin in the vinasse stoste before and after degrading toxin for the first time
Fruit
Illustrate: detection method is the detection method in national examination criteria, similarly hereinafter.
As can be seen from Table 3, after first time digests degradation toxin, vomitoxin and aflatoxin can be effective
Ground removal, and content meet vomitoxin content in feedstuff specified in national forage health standard GB13078-2017≤
5mg/Kg (5000 μ g/kg), aflatoxin minimum require to be≤10 μ g/kg.
Similarly, also the viscosity of the vinasse stoste before and after second of enzymatic hydrolysis degradation toxin is detected, detection knot
Fruit is shown in Table 4.
The viscosity measurements result of the vinasse stoste of second of table 4 enzymatic hydrolysis front and back
As can be seen from Table 4, after the second enzymolysis processing, the viscosity of wheat vinasse stoste is substantially reduced, and is had after being convenient for
It is continuous to be separated by solid-liquid separation operation, its separative efficiency is improved, to improve the yield of final byproduct.
Therefore, the resource utilization method of wheat vinasse of the present invention, by successively carrying out enzyme twice to wheat vinasse stoste
Solution operation, first digests the vomitoxin and aflatoxin in the wheat vinasse stoste that can degrade, so that subsequent to small
Brewer's grains gos deep into the byproduct of working process that state health standards, Ke Yizhi can be reached containing less amount of biotoxin
Connect use.Second of enzymatic hydrolysis can increase the content of the prebiotics such as its protein small peptide and xylo-oligosaccharide, meanwhile, it can also reduce wheat
The viscosity of vinasse stoste, and operated convenient for later separation, improve separative efficiency.
The above description is only a preferred embodiment of the present invention, is not intended to limit the scope of the invention, all at this
Under the inventive concept of invention, using equivalent structure transformation made by present specification, or directly/it is used in other indirectly
Relevant technical field is included in scope of patent protection of the invention.
Claims (10)
1. a kind of resource utilization method of wheat vinasse, which is characterized in that the resource utilization method packet of the wheat vinasse
Include following steps:
The wheat fecula that gives up is fermented, after distillation extracts ethyl alcohol, obtains wheat vinasse stoste；
The first enzyme preparation is added into wheat vinasse stoste, the toxin of enzymatic hydrolysis degradation for the first time is carried out, to remove the wheat vinasse
Biotoxin in stoste obtains enzymatic hydrolysis vinasse.
2. the resource utilization method of wheat vinasse as described in claim 1, which is characterized in that first enzyme preparation adds
Enter the 0.5%-3% that amount is the wheat vinasse stock solution quality.
3. the resource utilization method of wheat vinasse as described in claim 1, which is characterized in that first enzyme preparation is bud
Bacterium enzyme mix preparation, aflatoxins catabolic enzyme and the facultative aerobic character Bifidobacterium of spore bacillus and vomitoxin detoxification enzyme.
4. the resource utilization method of wheat vinasse as claimed in claim 3, which is characterized in that the bacillus and vomiting
Bacterium enzyme mix preparation, the aflatoxins catabolic enzyme and the mass ratio of the facultative aerobic character Bifidobacterium of detoxicated enzyme are (3-
6):(2-5):(0.5-2)。
5. the resource utilization method of wheat vinasse as described in claim 1, which is characterized in that the first time enzymatic hydrolysis degradation
The pH value of toxin is 3-7.5, and hydrolysis temperature is 20 DEG C -50 DEG C, and the enzyme digestion reaction time is 2h-8h.
6. the resource utilization method of the wheat vinasse as described in any one of claims 1 to 5, which is characterized in that " to small
The first enzyme preparation is added in brewer's grains stoste, the toxin of enzymatic hydrolysis degradation for the first time is carried out, to remove in the wheat vinasse stoste
Biotoxin, obtain enzymatic hydrolysis vinasse " the step of after further include:
The second enzyme preparation is added, carries out second and digests, to obtain secondary enzymolysis vinasse.
7. the resource utilization method of wheat vinasse as claimed in claim 6, which is characterized in that second enzyme preparation adds
Enter the 0.1%-0.5% that amount is the wheat vinasse stock solution quality.
8. the resource utilization method of wheat vinasse as claimed in claim 6, which is characterized in that second enzyme preparation is fibre
Tie up plain enzyme, xylo-oligosaccharide enzyme, protease and wall breaking enzyme.
9. the resource utilization method of wheat vinasse as claimed in claim 8, which is characterized in that the cellulase, described
The mass ratio of xylo-oligosaccharide enzyme, the protease and the wall breaking enzyme is (1-5): (10-30): (20-50): (1-10).
10. the resource utilization method of wheat vinasse as claimed in claim 6, which is characterized in that second of enzymatic hydrolysis
PH value is 3-7.5, and hydrolysis temperature is 50 DEG C -75 DEG C, and the enzyme digestion reaction time is 8h-12h.
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| CN115005328A (en) * | 2022-07-01 | 2022-09-06 | 东莞益海嘉里生物科技有限公司 | Vinasse stock solution treatment and reuse method |
| CN115669995A (en) * | 2022-11-01 | 2023-02-03 | 云南中烟工业有限责任公司 | Preparation method and application of tobacco humectant derived from vinasse macromolecules |
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