CN109865431A - A kind of film integrating device and protein matter separation method for protein matter separation concentration - Google Patents
A kind of film integrating device and protein matter separation method for protein matter separation concentration Download PDFInfo
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- CN109865431A CN109865431A CN201711245687.5A CN201711245687A CN109865431A CN 109865431 A CN109865431 A CN 109865431A CN 201711245687 A CN201711245687 A CN 201711245687A CN 109865431 A CN109865431 A CN 109865431A
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Abstract
The present invention relates to protein matter separating technologies, and in particular to a kind of film integrating device and protein matter separation method for protein matter separation concentration.Described device includes separation stoste slot, separative unit, cleaning systems unit and control unit；The separative unit includes level-one film separation unit, second level film separation unit.The present invention also provides the methods for carrying out separation concentration to protein matter with described device.Apparatus and method of the present invention uses the second-order separation system, not only realize the gradient separations of albumen, permeate can satisfy different product to the difference demand of different molecular weight of albumen, and system has automatic cleaning procedure, effectively save artificial investment, retain the effective component of solution system in system separation process, operating condition is mild, energy-efficient.
Description
Technical field
The present invention relates to protein matter separating technologies, and in particular to a kind of film collection for protein matter separation concentration
At device and protein matter separation method.
Background technique
Fibroin is called fibroin albumen, is the natural polymer fibrin extracted from silk, and content accounts for about silk
70%-80%, have good mechanical performance and physicochemical property, such as good flexibility and tensile strength, air-moisture-permeable
Property, slow release etc., and pass through the available different form of different disposal, such as fiber, solution, powder, film and gel, egg
White substance has preferable economic value.It is mentioned currently, more mature preparation process is generally degumming-hydrolysis-filtering
Pure-filtrate pH adjustment-concentration-sterilizing-finished product, but at present for most albumen systems, degumming work is passed through in preparation process
Powder is generally prepared into using low temperature cool-drying concentration after skill, but since stoste system protein content is not high, freezing is dry
Dry to need a large amount of refrigerating chamber, the processing time is longer, and energy consumption is higher.And albumen system complicated components, art methods can not
Meet the demand for the protein ingredient that product is distributed unimodal molecular weight in the market；Traditional lock out operation is (such as evaporation, extractive distillation
Deng) Processes and apparatus requirement is high, investment is big, energy consumption is high.
Patent CN105254731A discloses a kind of cocoon boiling wastewater fibroin concentration recycling system, passes through membrane distillation system
Cocoon boiling wastewater is concentrated 50-60 times by system, convenient for fibroin is purified reuse, will there is the silkworm of high value in the waste water of script discharge
Silk-fibroin recycles.But film distillation technology has that hydrophiling leakage and flux are universal relatively low.
CN101445546B disclose a kind of tussore silk fibroin solution preparation and its method for concentration, the wild silkworm fibroin of the patent it is water-soluble
Liquid is concentrated to get high concentration wild silkworm silk fibroin solution through semi-permeable membrane dehydration using high water absorption substance, and the method processing step is relatively complicated,
In addition the gradient separations of solution component be cannot achieve.
Summary of the invention
In consideration of it, it is necessary to provide regarding to the issue above it is a kind of for protein matter separation concentration film integrating device and
Protein matter preparation method.The device of the invention and preparation method effectively solve normal freeze-drying technology Protein Separation effect
The problems such as rate is low, concentration period is long, equipment energy consumption is high and gradient separations.
The present invention is using technical solution once:
A kind of film integrating device for protein matter separation concentration, including separation stoste slot, separative unit, system are clear
Wash unit and control unit；The separative unit includes level-one film separation unit, second level film separation unit, the separation stoste
Slot, level-one film separation unit and second level film separation unit pass sequentially through placed in series；The cleaning systems unit and separative unit
Pass through placed in series；Described control unit is electrically connected with separative unit and cleaning systems unit respectively, is controlled separative unit and is
Cleaning unit of uniting operates.
Further, the level-one film separation unit mainly include the first self priming pump, first check-valve, level-one membrane module,
First shut-off valve, first pressure gauge, the second shut-off valve；First self priming pump, first check-valve, level-one membrane module, first section
Only valve passes through placed in series；The first pressure gauge is set in the connecting pipe between level-one membrane module and the first shut-off valve,
For monitoring level-one membrane module internal pressure, prevent film from being destroyed；
The level-one membrane module is provided with the import of level-one membrane module, level-one membrane module exports, side port, one on level-one membrane module
Side port under grade membrane module；The level-one membrane module import passes through piping connection with first check-valve and the 7th shut-off valve respectively；Institute
The outlet of level-one membrane module is stated to connect by pipeline with the first shut-off valve；Side port passes through with the 6th shut-off valve on the level-one membrane module
Pipeline connection；Side port is connect with the second shut-off valve and third shut-off valve respectively by pipeline under the level-one membrane module；
The second level film separation unit mainly includes third shut-off valve, booster pump, second check-valve, second membrane module,
Four shut-off valves, second pressure gauge, the 5th shut-off valve；The third shut-off valve, booster pump, second check-valve, second membrane module,
Four shut-off valves pass through placed in series；The second pressure gauge is set to the connecting pipe between second membrane module and the 4th shut-off valve
On, for monitoring second membrane module internal pressure, prevent film from being destroyed；
The second membrane module be provided with second membrane module import, second membrane module outlet, side port on second membrane module,
Side port under second membrane module；The second membrane module import passes through piping connection with second check-valve and the 9th shut-off valve respectively；
The second membrane module outlet is connect by pipeline with the 4th shut-off valve；Side port and the 8th shut-off valve connect on the second membrane module
It connects；Side port is connect by pipeline with the 5th shut-off valve under the second membrane module；
The cleaning systems unit includes rinse bath, the second self priming pump, third check-valves, the 6th shut-off valve, the 7th cut-off
Valve, the 8th shut-off valve and the 9th shut-off valve；Second self priming pump and third check-valves pass through after placed in series；The third is stopped
Valve is returned to connect with the 6th shut-off valve and the 8th shut-off valve respectively by pipeline.
Further, the film integrating device for protein matter separation concentration further includes fore filter；It is described
Fore filter is connected between separation stoste slot and level-one film separation unit.
Further, the fore filter is stacked or screen mesh type filter.
Further, the level-one membrane module and described two of the film integrating device for protein matter separation concentration
Grade membrane module uses hollow fiber internal pressure film wire.
Further, the membrane material of the hollow-fibre membrane is polyvinyl chloride, Kynoar, polysulfones, polyether sulfone, polyamides
One or more of amine.
Further, there are gradient differences for the two-stage membrane module molecular cut off, according to different systems or actual demand
Using molecular cut off seperation film is not had to, there are 40,000-8 ten thousand dalton differences for the two-stage membrane module molecular cut off.
Further, the level-one membrane module is internal pressure hollow-fibre membrane, and molecular cut off is in 80,000-10 ten thousand dalton；Institute
The second membrane module stated is internal pressure hollow-fibre membrane, and molecular cut off is in 20,000-4 ten thousand dalton.
A method of condensing protein being separated using the film integrating device for protein matter separation concentration,
The following steps are included:
S1, solution to be separated is injected in separation stoste slot；
S2, first check-valve, the first shut-off valve, the second shut-off valve are opened, closes third shut-off valve, the 6th shut-off valve, the
Seven shut-off valves open the first self priming pump；
S3, first check-valve, the first shut-off valve are adjusted, the flow velocity for controlling liquid exists in 0.50-5.0m/s and pressure
Between 0.1Mpa-0.3Mpa, by-pass filtration liquid passes through side port under level-one membrane module and flows out via the second shut-off valve, can complete original
The primary concentration of liquid separates；
S4, concentration to be separated film integrating device level-one film separation unit it is stable after, i.e. level-one film separation unit
Stablize cyclic water outlet, bubble-free or without friction in water pump, successively open third shut-off valve, second check-valve, the 4th shut-off valve,
5th shut-off valve；Close the 8th shut-off valve, the 9th shut-off valve；
S5, booster pump is opened, adjusts second check-valve, the 4th shut-off valve, controls the flow velocity 0.5-5.0m/s and pressure of liquid
Power is between 0.1Mpa-0.3Mpa；Secondary filtration liquid passes through side port under second membrane module and flows out via the 5th shut-off valve, can be complete
It is separated at the secondary concentration of by-pass filtration liquid；
S6, concentration to be separated film integrating device level-one film separation unit and second level film separation unit it is stable, i.e.,
Level-one film separation unit and second level film separation unit stablize cyclic water outlet, and bubble-free or without friction in water pump produces water 0.5-
1.0h after permeant flux is decreased obviously, by control unit (PLC program automatic control), closes level-one film separation unit and two
Grade membrane separation unit operation successively opens third check-valves, the 6th shut-off valve, the 7th shut-off valve, the 8th shut-off valve, the 9th cut-off
Valve closes the first shut-off valve, the second shut-off valve, the 4th shut-off valve, the 5th shut-off valve, starts the second self priming pump, (can by cleaning solution
Select sodium hypochlorite) component is cleaned by backwash mode, cleaning waste liquid is discharged from the 7th shut-off valve, the 9th shut-off valve.
Further, the method for the separation condensing protein further includes replacement point after the completion of step S7:S1-S6 operation
It is pure water from feed liquid in stoste slot, is operated again by step S1-S5, device is just washed operation at least three times, and system is cleaned
Completely, in case next time uses.
The invention has the advantages that:
A kind of film integrating device for protein matter separation concentration of the present invention separates egg using secondary filtration
White class solution, fore filter can effectively remove impurity in system in described device, prevent membrane module lower end from blocking, effectively prolong
In addition long membrane module service life uses the second-order separation system, not only realize that the gradient separations of albumen, permeate can satisfy not
With product to the difference demand of different molecular weight of albumen, and system has automatic cleaning procedure, effectively saves artificial investment, body
It is the effective component for retaining solution system in separation process, operating condition is mild, and it is energy-efficient, it has a extensive future.
A kind of film integrating device for protein matter separation concentration of the present invention, separates silkworm using secondary filtration
Silk protein solution has carried out pilot scale research in protein production enterprise, has realized gradient separations to Protein Separation, can satisfy enterprise
Demand of the different product to different molecular weight albumen.Protein solution is operated by multistage filtering, efficient concentration may be implemented, significantly
Enterprises production efficiency is improved, device has effectively delayed fouling membrane process by the cleaning procedure of special highly effective, in addition, device is wanted
Disinfection treatment after paying special attention to end of run, in case the pollution of remaining albumen, influences product total quality.
Detailed description of the invention
Fig. 1 is the film integrating device structural schematic diagram of protein matter separation concentration of the invention.
Description of symbols:
1, stoste slot is separated；2, the first self priming pump；3, first check-valve；4, level-one membrane module import；5, level-one membrane module；
6, level-one membrane module exports；7, the first shut-off valve；8, side port under level-one membrane module；9, the second shut-off valve；10, third shut-off valve；
11, booster pump；12, second check-valve；13, second membrane module import；14, second membrane module；15, second membrane module exports；16,
4th shut-off valve；17, side port under second membrane module；18, the 5th shut-off valve；19, rinse bath；20, the second self priming pump；21, third
Check-valves；22, the 6th shut-off valve；23, side port on level-one membrane module；24, the 7th shut-off valve；25, the 8th shut-off valve；26, second level
Membrane module upper side mouth；27, the 9th shut-off valve；28, first pressure gauge；29, second pressure gauge；30, large aperture hollow-fibre membrane；
31, small-bore hollow-fibre membrane；32, fore filter.
Specific embodiment
The problem of solved in order to better illustrate the present invention, used technical solution and effect achieved, are now tied
It closes specific embodiment and related data is further described.It should be noted that the content of present invention is including but not limited to following implementation
Example and combinations thereof embodiment.
The film integrating device for protein matter separation concentration of the invention of embodiment 1
The film integrating device of the separation concentration includes that separation stoste slot 1, separative unit, cleaning systems unit and control are single
Member；The separative unit includes level-one film separation unit, second level film separation unit, the separation stoste slot 1, level-one UF membrane list
Member and second level film separation unit are sequentially connected in series；The cleaning systems unit is connected with separative unit；Described control unit respectively with
Separative unit is connected with cleaning systems unit, controls separative unit and cleaning systems cell operation.
The level-one film separation unit mainly includes the first self priming pump 2, first check-valve 3,5, first sections of level-one membrane module
Only valve 7, first pressure gauge 28, the second shut-off valve 9；First self priming pump 2, first check-valve 3,5, first sections of level-one membrane module
Only valve 7 passes through placed in series；The first pressure gauge 28 is set to the connecting tube between level-one membrane module 5 and the first shut-off valve 7
On road, for monitoring 5 internal pressure of level-one membrane module；
The level-one membrane module 5 is provided with level-one membrane module import 4, level-one membrane module exports side port on 6, level-one membrane module
23, side port 8 under level-one membrane module；The level-one membrane module import 4 is connected with first check-valve 3 and the 7th shut-off valve 24 by pipeline
It connects；The level-one membrane module outlet 6 is connect by pipeline with the first shut-off valve 7；Side port 23 and the 6th on the level-one membrane module
Shut-off valve 22 connects；Side port 8 is connected with the second shut-off valve 9 and third shut-off valve 10 respectively by pipeline under the level-one membrane module
It connects.
The separator can also include fore filter 32, and the fore filter 32 is connected to separation stoste slot 1
Between level-one film separation unit, the fore filter 32 uses stacked or screen mesh type filter, realizes the pre- of stoste
Filtering, preferably, system preferably first uses laminated filter, convenient for the clean and maintenance of whole system.
The second level film separation unit mainly includes third shut-off valve 10, booster pump 11, second check-valve 12, secondary membrane group
Part 14, the 4th shut-off valve 16, second pressure gauge 29, the 5th shut-off valve 18；The third shut-off valve 10, booster pump 11, second are stopped
It returns valve 12, second membrane module 14, the 4th shut-off valve 16 and passes through placed in series；The second pressure gauge 29 is set to second membrane module
14 and the 4th connecting pipe between shut-off valve 16 on, for monitoring 14 internal pressure of second membrane module.
The second membrane module 14 is provided with second membrane module import 13, second membrane module exports 15, second membrane module
Side port 17 under upper side port 26, second membrane module；The second membrane module import 13 ends with second check-valve 12 and the 9th respectively
Valve 27 passes through piping connection；The second membrane module outlet 15 is connect by pipeline with the 4th shut-off valve 16；The secondary membrane group
Side port 26 is connect with the 8th shut-off valve 25 on part；Side port 17 is connected by pipeline and the 5th shut-off valve 18 under the second membrane module
It connects.
The cleaning systems unit include rinse bath 19, the second self priming pump 20, third check-valves 21, the 6th shut-off valve 22,
7th shut-off valve 24, the 8th shut-off valve 25, the 9th shut-off valve 27.
Second self priming pump 20 and third check-valves 21 pass through placed in series；The third check-valves 21 passes through pipeline point
It is not connect with the 6th shut-off valve 22 and the 8th shut-off valve 25, cleaning systems unit is mainly used for internal pressure membrane module in completion system
Backwash operation.
The level-one membrane module 5 and the second membrane module of the film integrating device for protein matter separation concentration
14 use hollow fiber internal pressure film wire；The membrane material of the hollow-fibre membrane is polyvinyl chloride, Kynoar, polysulfones, polyethers
One or more of sulfone, polyamide.
There are gradient difference, the membrane apertures of level-one membrane module to be greater than second membrane module for the two-stage membrane module molecular cut off,
Specific membrane aperture is corresponding with the protein molecular weight of retention, is used according to different systems or actual demand and does not have to molecular cut off
Seperation film, there are 40,000-8 ten thousand dalton differences for the two-stage membrane module molecular cut off.Specifically, the level-one membrane module 5
For large aperture internal pressure hollow-fibre membrane 30, molecular cut off is in 80,000-10 ten thousand dalton；The second membrane module 14 is aperture
Diameter internal pressure hollow-fibre membrane 31, molecular cut off is in 20,000-4 ten thousand dalton；The level-one membrane module 5 and the secondary membrane
There are difference in size between the fenestra of component 14, realize that the gradient separations of albumen, the size of membrane aperture are not limited only to the present embodiment
The pore size mentioned, aperture selection are determined based on separation system and products thereof demand.
The level-one membrane module 5 and second membrane module 14 choose the group of unlike material and aperture according to system different demands
Part.For example polyvinylidene fluoride film high temperature resistant is better, and polyvinyl chloride is used not above 50 degrees Celsius；The selection in aperture is base
It is real using two-stage or multistage membrane module because gradient distribution is presented in protein component in separation system, great albumen to be separated
Existing gradient separations, the different molecular weight albumen purposes after separation are different.
Device of the present invention all uses PLC to control, and when permeant flux is reduced to original 50%, carries out automatic clear
It washes, using PLC control system, efficient operation is realized in system automation operation.
The method that embodiment 2 utilizes apparatus of the present invention protein isolate substance
Using the method for the device protein isolate substance of the embodiment of the present invention 1, step includes:
S1, solution to be separated is injected in separation stoste slot 1；
S2, first check-valve 3, the first shut-off valve 7, the second shut-off valve 9 are opened, closes third shut-off valve the 10, the 6th and ends
Valve 22, the 7th shut-off valve 24 open the first self priming pump 2；
S3, first check-valve 3, the first shut-off valve 7 being adjusted, the flow velocity for controlling liquid is 2.5m/s, pressure 0.1Mpa, one
Grade filtered fluid is flowed out by side port 8 under level-one membrane module via the second shut-off valve 9, and the primary concentration separation of stoste can be completed；
S4, concentration to be separated film integrating device level-one film separation unit it is stable after, i.e. level-one film separation unit
Stablize cyclic water outlet, bubble-free or without friction in water pump successively opens third shut-off valve 10, second check-valve 12, the 4th section
Only valve 16, the 5th shut-off valve 18；Close the 8th shut-off valve 25, the 9th shut-off valve 27；
S5, open booster pump 11, adjust second check-valve 12, the 4th shut-off valve 16, control liquid flow velocity be 1.5m/s,
Pressure is 0.1Mpa；Secondary filtration liquid passes through side port 17 under second membrane module and flows out via the 5th shut-off valve 18, can complete one
The secondary concentration separation of grade filtered fluid；
S6, concentration to be separated film integrating device level-one film separation unit and second level it is stable, i.e. level-one UF membrane
Unit and second level film separation unit stablize cyclic water outlet, and bubble-free or without friction in water pump produces water 0.5-1.0h, permeant flux
It after being decreased obviously, is controlled by PLC, closes level-one film separation unit and second level membrane separation unit operation, successively opened third and stop
Valve 21, the 6th shut-off valve 22, the 7th shut-off valve 24, the 8th shut-off valve 25, the 9th shut-off valve 27 are returned, the first shut-off valve 7, the are closed
Two shut-off valves 9, the 4th shut-off valve 16, the 5th shut-off valve 18 start the second self priming pump 20, and cleaning solution (optional sodium hypochlorite) is logical
It crosses backwash mode to clean component, cleaning waste liquid is discharged from the 7th shut-off valve 24, the 9th shut-off valve 27, closes system.
After the film integrating device each run of the protein matter separation concentration, by feed liquid in separation stoste slot 1
It is changed to pure water, S1-S5 operation is read again, operation is just being washed to level-one membrane module 5 and second membrane module 14 and its pipe-line system,
It is just washing no less than three times, is being then shut off system, in case next time uses.
When apparatus of the present invention are used for protein isolate, flow velocity should not be too high, and otherwise shearing force may destroy protein structure；Pressure
It is too high that not only film is impacted, it may accelerate fouling membrane process, it is optimal under 0.1Mpa, do not exceed 0.3Mpa；General flux
After decaying to original 50%, it is primary usually to comprehensively consider preferred operation 0.5-1h cleaning for Yao Jinhang cleaning operation.
Apparatus of the present invention can realize that replacing different membrane modules may be implemented difference by replacing the membrane module of different pore size
The selection of the separation of albumen, the membrane module in specific aperture is determined by the molecular size of required obtained protein.
The embodiments described above only express several embodiments of the present invention, and the description thereof is more specific and detailed, but simultaneously
Limitations on the scope of the patent of the present invention therefore cannot be interpreted as.It should be pointed out that for those of ordinary skill in the art
For, without departing from the inventive concept of the premise, various modifications and improvements can be made, these belong to guarantor of the invention
Protect range.Therefore, the scope of protection of the patent of the invention shall be subject to the appended claims.
Claims (10)
1. a kind of film integrating device for protein matter separation concentration, which is characterized in that single including separation stoste slot, separation
Member, cleaning systems unit and control unit；The separative unit includes level-one film separation unit, second level film separation unit, described
Separation stoste slot, level-one film separation unit and second level film separation unit are sequentially connected in series；The cleaning systems unit and separative unit
Series connection；Described control unit is connect with separative unit and cleaning systems unit, controls separative unit and cleaning systems cell operation.
2. the film integrating device according to claim 1 for protein matter separation concentration, which is characterized in that described one
Grade film separation unit mainly includes the first self priming pump, first check-valve, level-one membrane module, the first shut-off valve, first pressure gauge, the
Two shut-off valves；First self priming pump, first check-valve, level-one membrane module, the first shut-off valve pass through placed in series；Described first
Pressure gauge is set in the connecting pipe between level-one membrane module and the first shut-off valve；
The level-one membrane module is provided with the import of level-one membrane module, level-one membrane module exports, side port, level-one film on level-one membrane module
Side port under component；The level-one membrane module import and first check-valve and the 7th shut-off valve pass through piping connection；The level-one film
Module outlet is connect by pipeline with the first shut-off valve；Side port and the 6th shut-off valve are connected by pipeline on the level-one membrane module
It connects；Side port is connect with the second shut-off valve and third shut-off valve respectively by pipeline under the level-one membrane module；
The second level film separation unit mainly includes third shut-off valve, booster pump, second check-valve, second membrane module, the 4th section
Only valve, second pressure gauge, the 5th shut-off valve；The third shut-off valve, booster pump, second check-valve, second membrane module, the 4th section
Only valve passes through placed in series；The second pressure gauge is set in the connecting pipe between second membrane module and the 4th shut-off valve；
The second membrane module is provided with second membrane module import, second membrane module exports, side port, second level on second membrane module
Side port under membrane module；The second membrane module import and second check-valve and the 9th shut-off valve pass through piping connection；The second level
Membrane module outlet is connect by pipeline with the 4th shut-off valve；Side port is connect with the 8th shut-off valve on the second membrane module；It is described
Side port is connect by pipeline with the 5th shut-off valve under second membrane module；
The cleaning systems unit includes rinse bath, the second self priming pump, third check-valves, the 6th shut-off valve, the 7th shut-off valve,
Eight shut-off valves and the 9th shut-off valve；Second self priming pump and third check-valves pass through placed in series；The third check-valves is logical
Pipeline is crossed to connect with the 6th shut-off valve and the 8th shut-off valve respectively.
3. the film integrating device described in -2 any one for protein matter separation concentration according to claim 1, feature
It is, described device further includes fore filter；The fore filter is connected to separation stoste slot and level-one film separation unit
Between.
4. the film integrating device according to claim 3 for protein matter separation concentration, which is characterized in that before described
Filter is set using stacked or screen mesh type filter.
5. the film integrating device described in -2 any one for protein matter separation concentration according to claim 1, feature
It is, the level-one membrane module and the second membrane module use hollow fiber internal pressure film wire.
6. the film integrating device according to claim 5 for protein matter separation concentration, which is characterized in that in described
The membrane material of hollow fiber internal pressure film wire is one of polyvinyl chloride, Kynoar, polysulfones, polyether sulfone, polyamide or more
Kind.
7. the film integrating device according to claim 6 for protein matter separation concentration, which is characterized in that described one
The protein molecular weight of grade membrane module retention is greater than second membrane module, and there are 40,000-8 for the two-stage membrane module molecular cut off
Ten thousand dalton differences.
8. the film integrating device according to claim 7 for protein matter separation concentration, which is characterized in that described one
Grade membrane module molecular cut off is in 80,000-10 ten thousand dalton；The second membrane module molecular cut off is in 20,000-4 ten thousand dalton.
9. the film integrating device separation concentrate egg for utilizing claim 1-8 described in any item for protein matter separation concentration
The method of white matter, which comprises the following steps:
S1, solution to be separated is injected in separation stoste slot；
S2, first check-valve, the first shut-off valve, the second shut-off valve are opened, closes third shut-off valve, the 6th shut-off valve, the 7th section
Only valve opens the first self priming pump；
S3, first check-valve, the first shut-off valve are adjusted, controls the flow velocity and pressure of liquid, by-pass filtration liquid passes through level-one film group
Side port is flowed out via the second shut-off valve under part, can complete the primary concentration separation of stoste；
S4, concentration to be separated film integrating device level-one film separation unit it is stable after, successively open third shut-off valve, the
Two check-valves, the 4th shut-off valve, the 5th shut-off valve；Close the 8th shut-off valve, the 9th shut-off valve；
S5, booster pump is opened, adjusts second check-valve, the 4th shut-off valve, controls the flow velocity and pressure of liquid；Secondary filtration liquid is logical
It crosses side port under second membrane module to flow out via the 5th shut-off valve, the secondary concentration separation of by-pass filtration liquid can be completed；
S6, concentration to be separated film integrating device level-one film separation unit and the stable production water of second level for a period of time, produce water
After flux is decreased obviously, by control unit, level-one film separation unit and second level membrane separation unit operation are closed, successively opens the
Three check-valves, the 6th shut-off valve, the 7th shut-off valve, the 8th shut-off valve, the 9th shut-off valve close the first shut-off valve, the second cut-off
Valve, the 4th shut-off valve, the 5th shut-off valve start the second self priming pump, cleaning solution clean component by backwash mode, clearly
Waste liquid is washed to be discharged from the 7th shut-off valve, the 9th shut-off valve.
10. the method for separation condensing protein according to claim 9, which is characterized in that the method also includes steps
After the completion of S7:S1-S6 operation, feed liquid in replacement separation stoste slot is operated by step S1-S5, device is just being washed operation again.
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| CN111036085A (en) * | 2019-12-23 | 2020-04-21 | 青岛百发海水淡化有限公司 | Physical cleaning system and cleaning method for hollow fiber ultrafiltration membrane component |
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