CN109856641A - The terahertz detection method of poultry vivo biodistribution chip - Google Patents
The terahertz detection method of poultry vivo biodistribution chip Download PDFInfo
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- CN109856641A CN109856641A CN201910044325.2A CN201910044325A CN109856641A CN 109856641 A CN109856641 A CN 109856641A CN 201910044325 A CN201910044325 A CN 201910044325A CN 109856641 A CN109856641 A CN 109856641A
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Abstract
The embodiment of the present invention provides a kind of terahertz detection method of poultry vivo biodistribution chip, belongs to Terahertz applied technical field.This method comprises: poultry is placed on monitor station, poultry animal is scanned by the direct impulse based on THz wave, and obtains the scanning reflection signal that scanning obtains in real time；If occurring the reflection peak that step is greater than predetermined amplitude in the scanning reflection signal got in real time, then continue to scan on, when reflection peak disappears, the placing direction of transformation poultry continues to scan on, it is repeatedly scanned with until determining the edge shape of poultry vivo biodistribution chip according to scanning result up to default scanning times, and according to the edge shape of poultry vivo biodistribution chip, the position of biochip is determined.Due to using THz wave Detection Techniques, and THz wave will not generate harmful ionization to biological tissue, guarantee chicken safety, avoid people as eat harmful livestock and poultry and caused by injury.
Description
Technical field
The present embodiments relate to Terahertz applied technical field more particularly to a kind of terahertzs of poultry vivo biodistribution chip
Hereby detection method.
Background technique
During the large-scale cultivation of the agriculture poultry such as pig, ox, sheep, in order to preferably monitor the life of each individual poultry
Long situation generally can realize the identity of livestock and poultry individual and effective prison in entire breeding process in its et al. Ke biochip
Control makes Disease Warning Mechanism resolution so that poultry raiser is better understood by the growth course situation of livestock and poultry in advance.In Slaughter tache, need
Chip is taken out in time, to cause residual of the chip in meat products, causes foreign matter to remain, brings food safety hazards.
In general, after injecting biochip in livestock and poultry body, with the continuous growth of livestock and poultry individual, the position of chip in vivo can be sent out
Changing is butchered in meat products, processing link, needs to carry out the accurate positionin of biochip position by coherent detection means, so
Driving executing agency takes out from internal in time afterwards.Currently used detection means is based on x-ray imaging detection technique, because of X-ray
Radiation is big, belongs to ionising radiation, has certain safety radiation hidden danger to meat products, is badly in need of Development of Novel substitute technology.
Summary of the invention
To solve the above-mentioned problems, the embodiment of the present invention provides one kind and overcomes the above problem or at least be partially solved
State the terahertz detection method of the poultry vivo biodistribution chip of problem.
One side according to an embodiment of the present invention provides a kind of terahertz detection method of poultry vivo biodistribution chip,
Include:
Poultry is placed on monitor station, poultry animal is scanned by the direct impulse based on THz wave, and
The scanning reflection signal that scanning obtains is obtained in real time；
If occurring the reflection peak that step is greater than predetermined amplitude in the scanning reflection signal got in real time, continue to sweep
It retouches, when reflection peak disappears, the placing direction for converting poultry is continued to scan on, and it is repeatedly scanned with until reaching and presets scanning times, according to
Scanning result determines the edge shape of poultry vivo biodistribution chip, and according to the edge shape of poultry vivo biodistribution chip, determines
The position of biochip.
Method provided in an embodiment of the present invention passes through the spy based on THz wave by the way that poultry to be placed on monitor station
It surveys pulse pair poultry animal to be scanned, and obtains the scanning reflection signal that scanning obtains in real time.If the scanning got in real time
Occur the reflection peak that step is greater than predetermined amplitude in reflection signal, then continue to scan on, when reflection peak disappears, converts poultry
Placing direction continues to scan on, and is repeatedly scanned with until determining poultry vivo biodistribution chip according to scanning result up to default scanning times
Edge shape determine the position of biochip and according to the edge shape of poultry vivo biodistribution chip.Due to using terahertz
Hereby wave Detection Techniques, and THz wave will not generate harmful ionization to biological tissue, guarantee chicken safety, avoid people due to food
It is injured caused by with harmful livestock and poultry.In addition, since the subwavelength resonant structural unit of Terahertz and biochip generates solely
Special interaction characteristics greatly enhances the metal response signal intensity of detection livestock and poultry vivo biodistribution chip reflection, to greatly improve knowledge
Other accuracy rate.
It should be understood that above general description and following detailed description be it is exemplary and explanatory, can not
Limit the embodiment of the present invention.
Detailed description of the invention
In order to more clearly explain the embodiment of the invention or the technical proposal in the existing technology, to embodiment or will show below
There is attached drawing needed in technical description to be briefly described, it should be apparent that, the accompanying drawings in the following description is this hair
Bright some embodiments for those of ordinary skill in the art without creative efforts, can be with root
Other attached drawings are obtained according to these attached drawings.
Fig. 1 is a kind of process signal of terahertz detection method of poultry vivo biodistribution chip provided in an embodiment of the present invention
Figure；
Fig. 2 is a kind of structural schematic diagram of the reflective time-domain spectroscopy system of Terahertz provided in an embodiment of the present invention；
Fig. 3 is a kind of structural schematic diagram of biochip provided in an embodiment of the present invention；
Fig. 4 is that a kind of biochip provided in an embodiment of the present invention is added Meta Materials and the Contrast on effect of Meta Materials is not added to show
It is intended to.
Specific embodiment
In order to make the object, technical scheme and advantages of the embodiment of the invention clearer, below in conjunction with the embodiment of the present invention
In attached drawing, technical scheme in the embodiment of the invention is clearly and completely described, it is clear that described embodiment is
A part of the embodiment of the present invention, instead of all the embodiments.Based on the embodiments of the present invention, those of ordinary skill in the art
Every other embodiment obtained without creative efforts, shall fall within the protection scope of the present invention.
During the large-scale cultivation of the agriculture poultry such as pig, ox, sheep, in order to preferably monitor the life of each individual poultry
Long situation generally can realize the identity of livestock and poultry individual and effective prison in entire breeding process in its et al. Ke biochip
Control makes Disease Warning Mechanism resolution so that poultry raiser is better understood by the growth course situation of livestock and poultry in advance.In Slaughter tache, need
Chip is taken out in time, to cause residual of the chip in meat products, causes foreign matter to remain, brings food safety hazards.
In general, after injecting biochip in livestock and poultry body, with the continuous growth of livestock and poultry individual, the position of chip in vivo can be sent out
Changing is butchered in meat products, processing link, needs to carry out the accurate positionin of biochip position by coherent detection means, so
Driving executing agency takes out from internal in time afterwards.Currently used detection means is based on x-ray imaging detection technique, because of X-ray
Radiation is big, belongs to ionising radiation, has certain safety radiation hidden danger to meat products, is badly in need of Development of Novel substitute technology.
In recent years, Terahertz Technology is quickly grown.Terahertz (THz) wave typically refers to frequency range and is in 0.1-10THz
(1THz=1012Hz) the electromagnetic wave in range, due to its with a series of excellent special natures and wireless communication, radar,
The anti-fields such as probably of non-destructive testing, medical imaging and safety check have very extensive application prospect.It is to have for metal strongly in fact
Reflection characteristic.Especially, Terahertz photon energy is low, and the photon energy of 1THz is about (the 1/10 of X-ray 4meV6), it will not
Harmful ionization is generated to biological tissue, can safely carry out biomedical detection.
In general, biochip size is smaller, and as the weight of livestock and poultry is grown up, position can also become far from epidermis,
Into deep tissues, this has resulted in the decaying of probe response signal, has be easy to cause and fail to judge, and causes serious food safety hazards.
Metal array formula structure, i.e. metamaterial structure is generally presented in biochip.
Meta Materials refer to the equivalent uniform with the unexistent extraordinary physical characteristic of natural material artificial composite structure or
Material, also known as " metamaterial ", " meta-materials " or " metamaterials ".Usually by engineer, the period is uniformly distributed
, the metal micro structures of sub-wavelength dimensions composition, spy is realized by the coupling between these metal structures and incident electromagnetic wave
Fixed electromagnetic response.In brief, Meta Materials are exactly the resonant structure unit of sub-wavelength.When THz wave encounters this metal knot
When structure, response signal intensity can be significantly improved.
Existing frequently-used is X-ray detection technology, and damage of the X-ray to human body is mainly due to the characteristic of x-ray --- it is raw
Object effect.After roentgen radiation x body, it can make histocyte and body fluid that a series of variations occur, the cell in tissue is ionized radiation
Inactivation, so as to cause the radiolesion based on being damaged with hematopoietic tissue.Radiolesion degree exposure dosage, irradiated area
It is influenced with position, by according to individual with Various Complexes factors such as the energy of histiocytic radiation-sensitive rate and ray.Mainly
Damage such as non-irradiated acute and chronic radiation sickness, radioreaction, cardinal symptom is Nausea and vomiting, headache, hemogram abnormity, skin injury
It can lead to leukaemia, radiation cataract, cutaneum carcinoma etc. Deng, serious person, gestational period basis radioactive ray easily cause fetal-lethal, cause
Abnormal, serious feeblemindedness etc..Radiation, x-ray is big, can not only cause damage that can also have certain harm to livestock and poultry to human body.
Terahertz photon energy is low, and the photon energy of 1THz is about (the 1/10 of X-ray 4meV6), it will not be to biological tissue
Raw harmful ionization, can safely carry out biomedical detection, and general currently employed biochip is all periodic metal knot
Structure contains one layer of medium in the bottom of chip, can be considered as a kind of common Meta Materials.When THz wave is radiated on chip
When, electronics can be caused to shake, can resonated when electronics oscillation frequency is identical with the frequency of incident electromagnetic field, and then enter radio
The energy in magnetic field will be converted into the collective vibration energy of metal surface free electron, then just producing a kind of special electromagnetism
Mode.Electromagnetic field is limited in the range of the very little of metal surface and enhances, and then enhances detectable signal.
To sum up, compared to existing X-ray chip detection technique, Terahertz has: 1. photon ability is low, will not be to meat
Cause ionising radiation, guaranteed quality safety；2. strong reflection is presented to Terahertz in metal material, by detecting livestock and poultry vivo biodistribution core
Piece reflection metal response signal, it can be achieved that metal chip effective identification；3. the subwavelength resonant of Terahertz and biochip
Structural unit generates unique interaction characteristics, greatly enhances the metal response signal intensity of detection livestock and poultry vivo biodistribution chip reflection,
Greatly improve recognition accuracy.
Based on above description, the embodiment of the invention provides a kind of terahertz detection methods of poultry vivo biodistribution chip.
Referring to Fig. 1, this method comprises:
101, poultry is placed on monitor station, poultry animal is swept by the direct impulse based on THz wave
It retouches, and obtains the scanning reflection signal that scanning obtains in real time.
In 101, it can be detected by reflective terahertz time-domain system.Reflective terahertz time-domain spectroscopy system
System is the THz wave by reflecting on sample to realize the detection of signal, if using transmission-type terahertz time-domain frequency spectrograph
Words, since livestock and poultry figure is larger, during Terahertz passes through, signal decaying is too many, can generate biggish measurement error.And this
Inventive embodiments use reflective detection system to reduce the decaying of signal as far as possible.Typical terahertz time-domain system master
It to be made of ultrafast laser, Terahertz generator, sensitive detection parts and time delay module, the femtosecond which generates
The laser pulse mirror that is split first is divided into two-way, is pump light and detection light respectively.Pump light extends optical path by time delay module
Chopper is reached after distance, chopper is incident on the photoconductive antenna of Ga As material after being modulated to pump light and generates too
Hertz pulse.Pulse after off axis paraboloidal mirror focuses penetrate sample, then after off axis paraboloidal mirror focuses again and
Detection light is coaxially incident on nonlinear optical crystal ZnTe.And light namely direct impulse are detected for detecting the intracorporal life of poultry
Object chip.
If occurring the reflection peak that step is greater than predetermined amplitude in the scanning reflection signal 102, got in real time, continue
Scanning, when reflection peak disappears, the placing direction for converting poultry is continued to scan on, and is repeatedly scanned with until up to scanning times, root is preset
According to scanning result, the edge shape of poultry vivo biodistribution chip is determined, and according to the edge shape of poultry vivo biodistribution chip, really
Determine the position of biochip.
Specifically, direct impulse with through the terahertz pulse after sample synchronize enter THz radiation detection device, finally
The signal detected is converted to electric signal and exports after amplifying locking phase, when THz wave is radiated on chip, can cause
Electronics concussion, can resonate, and then the energy of incident electromagnetic field when electronics oscillation frequency is identical with the frequency of incident electromagnetic field
Amount will be converted into the collective vibration energy of metal surface free electron, then just producing a kind of special electromagnetic mode electromagnetism
Field is limited in the range of the very little of metal surface and enhances, and spectra values correspondingly can be observed and become larger, namely obtain
There is absorption peak in the scanning signal got.
Method provided in an embodiment of the present invention passes through the spy based on THz wave by the way that poultry to be placed on monitor station
It surveys pulse pair poultry animal to be scanned, and obtains the scanning reflection signal that scanning obtains in real time.If the scanning got in real time
Occur the reflection peak that step is greater than predetermined amplitude in reflection signal, then continue to scan on, when reflection peak disappears, converts poultry
Placing direction continues to scan on, and is repeatedly scanned with until determining poultry vivo biodistribution chip according to scanning result up to default scanning times
Edge shape determine the position of biochip and according to the edge shape of poultry vivo biodistribution chip.Due to using terahertz
Hereby wave Detection Techniques, and THz wave will not generate harmful ionization to biological tissue, guarantee chicken safety, avoid people due to food
It is injured caused by with harmful livestock and poultry.In addition, since the subwavelength resonant structural unit of Terahertz and biochip generates solely
Special interaction characteristics greatly enhances the metal response signal intensity of detection livestock and poultry vivo biodistribution chip reflection, to greatly improve knowledge
Other accuracy rate.
Content based on the above embodiment, as a kind of alternative embodiment, the photon energy force value of direct impulse is in terahertz
Hereby wave band, photon energy force value is lower than default secure threshold, not cause ionising radiation to the meat of poultry.
Content based on the above embodiment, as a kind of alternative embodiment, biochip includes subwavelength resonant structure list
The metal material of member；Correspondingly, reflection peak is metal response signal.
Content based on the above embodiment also includes subwavelength resonant knot in biochip as a kind of alternative embodiment
Structure unit, to enhance the intensity of metal response signal.
Content based on the above embodiment, as a kind of alternative embodiment, the embodiment of the present invention is not placed on to by poultry
Mode on monitor station specifically limits, including but not limited to: the light pulse generated based on femto-second laser, obtains pumping arteries and veins
Punching is based on pumping pulse, determines the set-point on monitor station, poultry is placed on set-point.
Content based on the above embodiment, as a kind of alternative embodiment, the embodiment of the present invention is not to based on femtosecond laser
The light pulse that device generates, the mode for obtaining pumping pulse specifically limit, including but not limited to: the light that femto-second laser is generated
Pulse obtains two pulses by beam splitter, wherein will pass through adjustable laser attenuator assembly by a beam pulse, and obtain pumping pulse.
Specifically, light pulse can be generated by ti:sapphire laser femto-second laser, is divided into two bundles light pulse, two-beam arteries and veins after first passing through beam splitter
Punching becomes the pulse of mean power 10mW by adjustable laser attenuator assembly respectively again.Wherein a beam pulse is as pumping pulse.
Content based on the above embodiment, as a kind of alternative embodiment, the embodiment of the present invention not to being based on pumping pulse,
Determine that the mode of the set-point on monitor station specifically limits, including but not limited to: time delay system is passed through into pumping pulse
Terahertz emission generation device is entered, the terahertz pulse of generation is collected by hemispherical silicon lens, by 8-F confocal system
Collimation and focusing, are compressed into hot spot at center, hot spot is set-point.Wherein, that hot spot is directly 3.5mm.
Content based on the above embodiment passes through the direct impulse pair based on THz wave as a kind of alternative embodiment
Before poultry animal is scanned, it is also based on the light pulse of femto-second laser generation, obtains direct impulse.
Content based on the above embodiment, as a kind of alternative embodiment, the embodiment of the present invention is not to based on femtosecond laser
The light pulse that device generates, the mode for obtaining direct impulse specifically limit, including but not limited to: the light that femto-second laser is generated
Pulse obtains two pulses by beam splitter, wherein will pass through adjustable laser attenuator assembly by an other beam pulse, and obtain detection arteries and veins
Punching.Wherein, the above process can be realized that the structure of the system can refer to Fig. 2 by the reflective time-domain spectroscopy system of Terahertz.Fig. 2
The transmitting and reception of middle THz-TDS experimental provision are all based on GaAs photoconductive antenna, and the core optical path of device is off-axis by 4
The 8-F confocal geometrical system composition of paraboloidal mirror composition, pump light and detection light are mixed by central wavelength 800nm, pulsewidth 25fs
Titanium sapphire self mode locked fs laser provides, and the effective bandwidth of whole device is 0.1-4.5THz, signal-to-noise ratio 15000:1,
The smallest lateral dimension of sample to be tested is 3.5mm.In addition, 0.8mm × 0.8mm biochip and interior structure can refer to Fig. 3.Its
In, the circular granular radius on chip is 100nm.Meta Materials are added can refer to the reflectivity comparison for tearing material chip open is not added
Fig. 4.
Content based on the above embodiment, as a kind of alternative embodiment, the edge shape of poultry vivo biodistribution chip is
Plane polygon；Correspondingly, the embodiment of the present invention determines biochip not to the edge shape according to poultry vivo biodistribution chip
The mode of position specifically limit, including but not limited to: determining the plane polygon of biochip, will appoint in plane polygon
Meaning vertex is source point, and source point is connected all non-adjacent vertex in plane polygon, plane polygon is divided into several
A triangle；The area and barycentric coodinates for determining each triangle calculate each barycenter oftriangle abscissa and each triangle
The first product between the area of shape, and first and value of the first product of all triangles are calculated, calculate all gores
Long-pending area and value calculates every using the ratio between first and value and area and value as the center of gravity abscissa of plane polygon
The second product between one barycenter oftriangle ordinate and the area of each triangle, and calculate the second of all triangles and multiply
Second long-pending and value, using the ratio between second and value and area and value as the center of gravity ordinate of plane polygon；Based on flat
The center of gravity abscissa and center of gravity ordinate of face polygon, determine the center of gravity of biochip, and the position as biochip.
The apparatus embodiments described above are merely exemplary, wherein described, unit can as illustrated by the separation member
It is physically separated with being or may not be, component shown as a unit may or may not be physics list
Member, it can it is in one place, or may be distributed over multiple network units.It can be selected according to the actual needs
In some or all of the modules achieve the purpose of the solution of this embodiment.Those of ordinary skill in the art are not paying creativeness
Labour in the case where, it can understand and implement.
Through the above description of the embodiments, those skilled in the art can be understood that each embodiment can
It realizes by means of software and necessary general hardware platform, naturally it is also possible to pass through hardware.Based on this understanding, on
Stating technical solution, substantially the part that contributes to existing technology can be embodied in the form of software products in other words, should
Computer software product may be stored in a computer readable storage medium, such as ROM/RAM, magnetic disk, CD, including several fingers
It enables and using so that a computer equipment (can be personal computer, server or the network equipment etc.) executes each implementation
Method described in certain parts of example or embodiment.
Finally, it should be noted that the above embodiments are merely illustrative of the technical solutions of the present invention, rather than its limitations；Although
Present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that: it still may be used
To modify the technical solutions described in the foregoing embodiments or equivalent replacement of some of the technical features；
And these are modified or replaceed, technical solution of various embodiments of the present invention that it does not separate the essence of the corresponding technical solution spirit and
Range.
Claims (9)
1. a kind of terahertz detection method of poultry vivo biodistribution chip characterized by comprising
Poultry is placed on monitor station, poultry animal is scanned by the direct impulse based on THz wave, and in real time
Obtain the scanning reflection signal that scanning obtains；
If occurring the reflection peak that step is greater than predetermined amplitude in the scanning reflection signal got in real time, continue to scan on, when
When reflection peak disappears, the placing direction for converting the poultry is continued to scan on, and is repeatedly scanned with until up to default scanning times, according to sweeping
The edge shape as a result, the determining poultry vivo biodistribution chip is retouched, and according to the edge shape of the poultry vivo biodistribution chip
Shape determines the position of the biochip.
2. the method according to claim 1, wherein the photon energy force value of the direct impulse is in THz wave
Section, the photon energy force value are lower than default secure threshold, cause ionising radiation with the meat not to the poultry.
3. the method according to claim 1, wherein the biochip includes subwavelength resonant structural unit
Metal material；Correspondingly, the reflection peak is metal response signal.
4. the method according to claim 1, wherein described be placed on poultry on monitor station, comprising:
Based on the light pulse that femto-second laser generates, pumping pulse is obtained, the pumping pulse is based on, determines on the monitor station
Set-point, the poultry is placed on the set-point.
5. according to the method described in claim 4, it is characterized in that, the light pulse generated based on femto-second laser, is obtained
Pumping pulse, comprising:
The light pulse that the femto-second laser is generated obtains two pulses by beam splitter, will wherein a beam pulse by can
The laser attenuator assembly of tune obtains the pumping pulse.
6. according to the method described in claim 4, it is characterized in that, it is described be based on the pumping pulse, determine the monitor station
On set-point, comprising:
The pumping pulse is entered into terahertz emission generation device by time delay system, the terahertz pulse of generation by
Hemispherical silicon lens is collected, and collimation and focusing by 8-F confocal system are compressed into hot spot at center, and the hot spot is institute
State set-point.
7. the method according to claim 1, wherein it is described by the direct impulse based on THz wave to poultry
Before animal is scanned, further includes:
Based on the light pulse that femto-second laser generates, the direct impulse is obtained.
8. the method according to the description of claim 7 is characterized in that the light pulse generated based on femto-second laser, is obtained
The direct impulse, comprising:
The light pulse that the femto-second laser is generated obtains two pulses by beam splitter, will wherein an other beam pulse pass through
Adjustable laser attenuator assembly is crossed, the direct impulse is obtained.
9. the method according to claim 1, wherein the edge shape of the poultry vivo biodistribution chip is plane
Polygon；Correspondingly, the edge shape according to the poultry vivo biodistribution chip, determines the position of the biochip,
Include:
It is source point by vertex any in the plane polygon, the source point is connected to all non-phases in the plane polygon
The plane polygon is divided into several triangles by adjacent vertices；
The area and barycentric coodinates for determining each triangle calculate the face of each barycenter oftriangle abscissa and each triangle
The first product between product, and first and value of the first product of all triangles are calculated, calculate the face of all triangle areas
Product and value, using the ratio between described first and value and the area and value as the center of gravity abscissa of the plane polygon,
The second product between each barycenter oftriangle ordinate and the area of each triangle is calculated, and calculates all triangles
Second and value of the second product, using the ratio between described second and value and the area and value as the plane polygon
Center of gravity ordinate；
Center of gravity abscissa and center of gravity ordinate based on the plane polygon, determine the center of gravity of the biochip, and conduct
The position of the biochip.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110584671A (en) * | 2019-09-22 | 2019-12-20 | 萧县华恒静电科技有限公司 | Metal detection device for pet body |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110584671A (en) * | 2019-09-22 | 2019-12-20 | 萧县华恒静电科技有限公司 | Metal detection device for pet body |
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