CN109856332A - The method that comprehensive descision plant absorption utilizes moisture - Google Patents
The method that comprehensive descision plant absorption utilizes moisture Download PDFInfo
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- CN109856332A CN109856332A CN201910093405.7A CN201910093405A CN109856332A CN 109856332 A CN109856332 A CN 109856332A CN 201910093405 A CN201910093405 A CN 201910093405A CN 109856332 A CN109856332 A CN 109856332A
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Abstract
The invention discloses a kind of methods that comprehensive descision plant absorption utilizes moisture, belong to the detection method that plant absorption utilizes moisture.Its method is: will grind after plant leaf blade drying, measures its C-O isotopes fractionation factor respectively, calculate separately the Stable carbon-isotope and oxygen-isotope ratio of blade, establish the Stable carbon-isotope and oxygen-isotope ratio relation model δ of blade18O=m δ13C+n, by formula W UE=Ca [b- (δ13Ca‑δ13Cp)/(1+δ13Cp/1000)]/[1.6 (b-a)] comparison judges that situation is absorbed and utilized in plant moisture.The method of the present invention can quick and precisely judge in growing process, certain section of time implants absorb moisture and the case where using moisture, and will not damage to damaging property of plant；It is a kind of integrated approach that characterization plant moisture is absorbed and utilized.
Description
Technical field
The present invention relates to a kind of plant absorptions to be absorbed using the detection method of moisture more particularly to a kind of characterization plant moisture
The integrated approach utilized.
Background technique
Different Soil Water Deficits can directly threaten the nutrient absorption and growth and development of plant.In recent decades, many areas
Because decrease in precipitation, soil moisture reduce, moisture has become the Main Factors of limitation desert areas plant life and annidation；
Therefore limited water resources are rationally and effectively absorbed and utilized is that plant survives steadily in the long term, adapts to adverse circumstances and vegetation restoration and reconstruction
Important prerequisite.
Root system of plant to the absorption of moisture is carried out along the direction that water potential gradient between soil and root system xylem declines.Mesh
Before, the waterpower degree of leading of root system is commonly used to characterize water uptake by plant roots ability, but digs out since the method needs to connect plant root, is right
Plant damages, therefore is typically only capable to be suitable for the culture experiments such as potting, and field grown plant is then not suitable for.In addition,
The ratio of Photosynthetic Rate and transpiration rate is mostly used at present to reflect plant water use efficiency (Water Use
Efficiency, WUE), but the instantaneity of its measurement is not easy to connect with the productivity that plant is final.
The driving force of water uptake by plant roots mostlys come from the transpiration pull of plant leaf blade.Green plants passes through photosynthesis
Absorb CO2With water and synthesis of organic substance, the oxygen stable isotope ratio delta of cellulose18Oxygen stable isotope ratio of the O by its source water
Value δ18O control, rather than by the absorbed CO of Stoma of Leaves2In oxygen stable isotope ratio delta18O is determined；Therefore, plant is organic
The oxygen stable isotope ratio delta of matter18O, which has recorded, utilizes the information of ambient water oxygen stable isotope composition in growing process.
During absorbing moisture and being transmitted to xylem isotope fractionation, but the transpiration of blade surface do not occur for root system of plant
It will cause oxygen stable isotope ratio delta18O is fractionated.Thus, the oxygen stable isotope ratio delta of plant tissue18O is able to reflect
Transpiration, evaporation conditions when substance formation, can be by the oxygen stable isotope ratio delta of plant leaf blade18O is as characterization plant water suction
A kind of Substitute Indexes of ability power.Very important, plant transpiration effect is too strong, and when photosynthesis is weaker can cause to plant
Object WUE is reduced, and is unfavorable for plant and is survived steadily in the long term.In recent years, stable carbon isotope techniques international WUE research in be compared with
For common measuring method.Due to carbon stable isotope ratio delta13C and blade intercellular CO2The relationship and WUE of concentration (Ci) with
The presence of correlativity between Ci, carbon stable isotope ratio delta13C can be used to indicate the height of plant WUE.In general, plant
Carbon stable isotope ratio delta13C is more negative, and plant WUE is lower, i.e. the two correlation.Therefore, it is possible to by plant leaf blade
Carbon stable isotope ratio delta13Substitute Indexes of the C as plant WUE.Meanwhile the carbon stable isotope ratio delta of plant leaf blade13The variation of C can distinguish photosynthetic rate and stomatal conductance changes, because of the oxygen stable isotope ratio delta of plant leaf blade18The variation of O
Independently of photosynthetic rate, provided information only characterizes the variation of stomatal conductance, thus the carbon stable isotope of plant leaf blade
Ratio delta13C, oxygen stable isotope ratio delta18O conceptual model can also differentiate that photosynthetic rate and stomatal conductance influence WUE.
Summary of the invention
In order to overcome the above-mentioned deficiencies of the prior art, the present invention is intended to provide a kind of comprehensive descision plant absorption utilizes moisture
Method, this method can characterize a period of time implants moisture absorption and utilization efficiency.
To achieve the goals above, steps are as follows for the technical solution adopted by the present invention:
1) the quasi- comparison of acquisition judges the sunshine leaf piece of plant, claims fresh weight, then dries under the conditions of 60 DEG C to constant weight, obtain sample
Product；
2) sample claims dry weight, and 60 meshes are crossed in grinding, is sealed；
3) the carbon potential element fractionation factor of sample is measured respectively13C/12C, OXYGEN ISOTOPE FRACTIONATION coefficient18O/16O；
4) using PDB as carbon isotope reference substance, using SMOW as oxygen isotope reference substance, by formula δX(‰)=
(Rsa-Rst)/Rst × 1000 calculate separately the Stable carbon-isotope and oxygen-isotope ratio of sample；In formula, δXFor the δ of sample13C or δ18O,
Rsa is sample13C/12C or18O/16O, Rst are carbon isotope reference substance13C/12C or oxygen isotope reference substance18O/16O；
5) the carbon stable isotope ratio delta of sample is established13C and oxygen stable isotope ratio delta18The relational model of O: δ18O=
mδ13C+n；
In formula, m and n are respectively practical calculating slope and intercept；
6) according to the Stable carbon-isotope and oxygen-isotope ratio delta of sample13C、δ18O comparison judges that situation is absorbed and utilized in plant moisture:
WUE=Ca [b- (δ13Ca-δ13Cp)/(1+δ13Cp/1000)]/[1.6(b-a)]；
Wherein, Ca is Atmospheric CO2Air pressure can be consulted from website (http://www.esrl.noaa.gov) and be obtained, can also
It is monitored according to local climate；δ13Ca=-6.429-0.0060exp [0.0217 (t-1740)] is stable carbon isotope in atmosphere
Abundances；δ13Cp is the carbon stable isotope ratio delta of sample13C；A=4.4 ‰, b=27 ‰, are empirical, respectively table
Show diffusion and the carbon stable isotope fractionation value that carboxylation reaction generates；The t expression of years.
Compared with the prior art, the method for the present invention utilizes plant leaf blade organic matter oxygen stable isotope ratio delta18O instruction is planted
Object root water uptake situation, while establishing δ18The carbon stable isotope ratio delta of O and plant leaf blade13The relational model of C can integrate
Reflect that photosynthetic rate and stomatal conductance influence WUE.For conventional method, the method for the present invention can not destroy vegetation root
In the case of system, can quick and precisely it judge in growing process, certain section of time implants absorb moisture and the feelings using moisture
Condition；Operation was not only simple but also convenient.
Detailed description of the invention
Fig. 1 be in the case that soil water supply sufficient (WW), wane (ND), using the method for the present invention judgement inoculation, be not inoculated with
Oxygen stable isotope ratio delta between arbuscular mycorrhizal fungi mulberry leaf18O relational graph；
Fig. 2 be in the case that soil water supply sufficient (WW), wane (ND), using conventional method measure soil water content,
Mulberry tree root system, stalk, leaf water content respectively with the oxygen stable isotope ratio delta of mulberry leaf18The relational graph of O；
Fig. 3 be in the case that soil water supply sufficient (WW), wane (ND), using the method for the present invention judgement inoculation, be not inoculated with
Carbon stable isotope ratio delta between arbuscular mycorrhizal fungi mulberry leaf13C relational graph；
Fig. 4 be in the case that soil water supply sufficient (WW), wane (ND), using the method for the present invention judgement inoculation, be not inoculated with
WUE relational graph between arbuscular mycorrhizal fungi mulberry leaf；
Fig. 5 be in the case that soil water supply sufficient (WW), wane (ND), use conventional method measure soil water content with
And mulberry tree root system, stalk, leaf water the content relational graph with mulberry leaf WUE respectively；
Fig. 6 be in the case that soil water supply sufficient (WW), wane (ND), the carbon stabilization of mulberry leaf judge using the method for the present invention
Isotopic ratio δ13C and oxygen stable isotope ratio delta18The relational graph of O.
Specific embodiment
Mulberry tree moisture absorption can be promoted to be utilized as specific embodiment with judgement verifying Arbuscular Mycorrhizal Fungi below,
And the invention will be further described in conjunction with attached drawing attached drawing；Its step are as follows:
1) it will be respectively charged into three plastic barrels by the soil of sterilizing, guarantee depth of soil to the 2/3 of plastic barrel height
Place；
2) three plants of consistent mulberry saplings of growing way are chosen, it is mould that the root system of one plant of mulberry sapling is uniformly dipped in into full Moses's pipe handle capsule
(Funneliformis mosseae, Fm) microbial inoculum, that the root system of another plant of mulberry sapling is uniformly dipped in full Gen Neigensheng capsule is mould
The root system of third group strain mulberry sapling is uniformly dipped in full process sterilizing microbial inoculum by (Rhizophagus intraradices, Ri) microbial inoculum；
3) three plants of mulberry saplings are transplanted respectively into each plastic barrel, and remaining Moses's pipe handle capsule mould agent, Gen Neigensheng capsule is mould
Microbial inoculum, sterilizing microbial inoculum are poured into respectively in above-mentioned corresponding plastic barrel, and remaining sterile soil is respectively charged into each plastic barrel, is cultivated
Mulberry tree；Guarantee the sterile soil in each plastic barrel be 3kg, microbial inoculum 30g；
4) at the end of mulberry tree each growth phase, using soil moisture tester on-site measurement soil water content, and
Acquire mulberry tree root system, stalk, blade；
5) the mulberry tree root system cleaning and wiping of acquisition is done, claims fresh weight (to be accurate to together with the mulberry tree stalk of acquisition, leaf
0.0001g), drying to constant weight under the conditions of 60 DEG C (about needing 48h), obtains dry product；
6) dry weight (being accurate to 0.0001g) for quickly weighing the dry product is ground rapidly with mortar, crosses 60 meshes, then
It is packed into vial to be sealed, obtains test sample；
7) carbon potential element fractionation factor is measured using stable isotope mass spectrograph (Thermo Fisher MAT 253plus)13C/12C, OXYGEN ISOTOPE FRACTIONATION coefficient18O/16O；
8) using PDB as carbon isotope reference substance, using SMOW as oxygen isotope reference substance, by formula δX(‰)=
(Rsa-Rst)/Rst × 1000 calculate separately the carbon stable isotope ratio delta of test sample13C and oxygen stable isotope ratio delta18O；
In formula, Rsa is test sample13C/12C or18O/16O, Rst are carbon isotope reference substance13C/12C or the same position of oxygen
Plain reference substance18O/16O；
9) the carbon stable isotope ratio delta of test sample is established13C and oxygen stable isotope ratio delta18The relational model of O:
δ18O=m δ13C+n；
In formula, m and n are respectively practical calculating slope and intercept；
10) according to the carbon stable isotope ratio delta of test sample13C, oxygen stable isotope ratio delta18O comparison judges plant
Moisture absorption utilization power:
WUE=Ca [b- (δ13Ca-δ13Cp)/(1+δ13Cp/1000)]/[1.6(b-a)]；
Wherein, Ca is Atmospheric CO2Air pressure can be consulted from website (http://www.esrl.noaa.gov) and be obtained, can also
It is monitored according to local climate；δ13Ca=-6.429-0.0060exp [0.0217 (t-1740)] is stable carbon isotope in atmosphere
Abundances；δ13Cp is the carbon stable isotope ratio delta of sample13C；A=4.4 ‰, b=27 ‰, are empirical, respectively table
Show diffusion and the carbon stable isotope fractionation value that carboxylation reaction generates；The t expression of years.
The present embodiment testing result is as shown in Fig. 1,3,4,6.
It will be seen from figure 1 that being inoculated with the oxygen stable isotope ratio delta of the mulberry leaf of Fm and Ri under the conditions of soil normal water supply18O significantly reduces 2.38% and 2.00% respectively.When Different Soil Water Deficits, it is inoculated with the oxygen stable isotope ratio of the mulberry leaf of Fm and Ri
Value δ18O significantly reduces 4.98% and 1.14% respectively.From the point of view of the entire growth course of mulberry tree, the oxygen with mulberry leaf under treatment conditions is steady
Determine isotopic ratio δ18O is also significantly reduced, wherein the oxygen stable isotope ratio delta of the mulberry leaf of inoculation Fm and Ri18O declines respectively
6.45% and 3.04%.
Fig. 2 is shown: under the conditions of normal water supply, mulberry leaf δ18The moisture related coefficient of O and soil, Mulberry Roots, stem, leaf is respectively
0.73 (P=0.03, N=9), -0.78 (P=0.01, N=9), -0.77 (P=0.02, N=9), -0.58 (P=0.10, N=
9).Under natural arid, with soil, Mulberry Roots, stem, leaf moisture related coefficient be respectively 0.81 (P=0.01, N=9) ,-
0.55 (P=0.13, N=9), -0.68 (P=0.04, N=9), -0.59 (P=0.09, N=9).
Fig. 1,2 show the oxygen stable isotope ratio delta of mulberry leaf18O is significantly positively correlated with soil moisture, with mulberry tree difference group
Knit moisture negative correlation；The oxygen stable isotope ratio delta of blade organic matter18O reflects plant moisture Change of absorption.
When can be seen that soil moisture abundance from Fig. 3,4 and wane, the carbon stable isotope ratio delta of mulberry leaf13C and WUE are equal
Inoculation Fm > inoculation Ri > is shown as not to be inoculated with.Wherein, when normal water supply, compared with not being inoculated with, the carbon for being inoculated with the mulberry leaf of Fm is stablized
Isotopic ratio δ13C and WUE increase separately 2.45% and 10.8%, and be inoculated with Ri increases separately 1.71% and 7.60%；Divide and loses
After lacking, inoculation Fm increases separately 1.82% and 6.83%, and be inoculated with Ri increases separately 0.63% and 2.32%.
Fig. 5 is shown: when normal water supply, WUE and soil, mulberry tree root system, stalk, the related coefficient difference of leaf water content
For -0.92 (P=0.00, N=9), 0.97 (P=0.00, N=9), 0.93 (P=0.00, N=9) and 0.53 (P=0.14, N=
9).Stop after supplying water, WUE and the related coefficient of soil, mulberry tree root system, stalk, leaf water content are respectively -0.92 (P=
0.00, N=9), 0.73 (P=0.03, N=9), 0.92 (P=0.00, N=9) and 0.77 (P=0.02, N=9).
Fig. 3,4,5 show that WUE and soil moisture are significantly negatively correlated, and are positively correlated with mulberry tree moisture.
Fig. 6 is shown, during growing of mulberry, no matter soil moisture is sufficient or water deficit, the carbon of mulberry leaf stablize same position
Plain ratio delta13C and oxygen stable isotope ratio delta18O is significantly negatively correlated, and related coefficient is respectively -0.81 (P=0.01, N=9)
With -0.82 (P=0.01, N=9).The relationship of the two specification of a model mulberry tree WUE is synchronous by photosynthetic and rising influence.
Claims (1)
1. a kind of method that comprehensive descision plant absorption utilizes moisture, it is characterised in that steps are as follows:
1) the quasi- comparison of acquisition judges the sunshine leaf piece of plant, claims fresh weight, then dries under the conditions of 60 DEG C to constant weight, obtain sample；
2) claim sample dry weight, 60 meshes are crossed in grinding, are sealed；
3) the carbon potential element fractionation factor of sample is measured respectively13C/12C, OXYGEN ISOTOPE FRACTIONATION coefficient18O/16O；
4) using PDB as carbon isotope reference substance, using SMOW as oxygen isotope reference substance, by formula δX(‰)=(Rsa-
Rst)/Rst × 1000 calculate separately the Stable carbon-isotope and oxygen-isotope ratio of sample；In formula, δXFor the δ of sample13C or δ18O, Rsa are
Sample13C/12C or18O/16O, Rst are carbon isotope reference substance13C/12C or oxygen isotope reference substance18O/16O；
5) the carbon stable isotope ratio delta of sample is established13C and oxygen stable isotope ratio delta18The relational model of O: δ18O=m δ13C
+n；
In formula, m and n are respectively practical calculating slope and intercept；
6) according to the Stable carbon-isotope and oxygen-isotope ratio delta of sample13C、δ18O comparison judges that situation is absorbed and utilized in plant moisture:
WUE=Ca [b- (δ13Ca-δ13Cp)/(1+δ13Cp/1000)]/[1.6(b-a)]；
Wherein, Ca is Atmospheric CO2Air pressure can be consulted from website (http://www.esrl.noaa.gov) and be obtained, can also be according to working as
Ground climate monitoring；δ13Ca=-6.429-0.0060exp [0.0217 (t-1740)] is stable carbon isotope Abundances in atmosphere；
δ13Cp is the carbon stable isotope ratio delta of sample13C；A=4.4 ‰, b=27 ‰, are empirical, respectively indicate diffusion and make
With the carbon stable isotope fractionation value generated with carboxylation reaction；The t expression of years.
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Cited By (3)
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| CN111948372A (en) * | 2020-07-10 | 2020-11-17 | 中国地质大学（武汉） | Method for identifying vegetation water utilization in arid region |
| CN112772304A (en) * | 2021-01-14 | 2021-05-11 | 中国科学院地理科学与资源研究所 | Method for identifying vegetation water utilization in desert area |
| CN113416650A (en) * | 2021-05-06 | 2021-09-21 | 江西省水利科学院 | Water-retaining plant growth-promoting bacterium, propagation method and application thereof |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN111948372A (en) * | 2020-07-10 | 2020-11-17 | 中国地质大学（武汉） | Method for identifying vegetation water utilization in arid region |
| CN112772304A (en) * | 2021-01-14 | 2021-05-11 | 中国科学院地理科学与资源研究所 | Method for identifying vegetation water utilization in desert area |
| CN112772304B (en) * | 2021-01-14 | 2022-11-15 | 中国科学院地理科学与资源研究所 | Method for identifying vegetation water utilization in desert area |
| CN113416650A (en) * | 2021-05-06 | 2021-09-21 | 江西省水利科学院 | Water-retaining plant growth-promoting bacterium, propagation method and application thereof |
| CN113416650B (en) * | 2021-05-06 | 2023-08-11 | 江西省水利科学院 | Water-retaining plant growth-promoting bacterium, propagation method and application thereof |
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