CN109855734B - Method for indirectly measuring spectral reflectance of insect body surface - Google Patents

Method for indirectly measuring spectral reflectance of insect body surface Download PDF

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Publication number
CN109855734B
CN109855734B CN201811611762.XA CN201811611762A CN109855734B CN 109855734 B CN109855734 B CN 109855734B CN 201811611762 A CN201811611762 A CN 201811611762A CN 109855734 B CN109855734 B CN 109855734B
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spectral
correction plate
insect
spectral reflectance
radiometer
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CN109855734A (en
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徐鹏
汪杭军
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Zhuji Xiangpeng Knitting Co ltd
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Jiyang College of Zhejiang A&F University
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Abstract

The invention discloses a method for usingThe method for indirectly measuring the spectral reflectance of the body surface of the insect comprises the following steps: measuring the spectral reflectance of the surface of the correction plate by using a spectrophotometer to obtain the spectral reflectance r of the surface of the correction plate; placing a light source on each of the left side and the right side of the correction plate, and projecting light rays of the two light sources onto the surface of the correction plate, wherein the normal lines of the two light sources and the surface of the correction plate are at an angle of 45 degrees and have the same distance with the center of the correction plate; placing a spectral radiometer right in front of the correction plate and on the same side with the two light sources, wherein the measurement direction of the spectral radiometer is perpendicular to the surface of the correction plate, and illumination and measurement geometric conditions of 45 degrees x 0 degrees are formed between the spectral radiometer and the light sources; measuring spectral radiance l reflected by insect body surface by using spectral radiometersAnd the spectral radiance l reflected by the surface of the correction platec(ii) a Calculating the spectral reflectance of the surface of the insect body. The invention has the characteristics of simple and convenient operation, easy implementation and capability of ensuring the measurement precision.

Description

Method for indirectly measuring spectral reflectance of insect body surface
Technical Field
The invention relates to the technical field of object surface reflection spectrum measurement, in particular to a method for indirectly measuring the insect body surface spectrum reflectance, which is simple and convenient to operate and easy to implement and can ensure the measurement precision.
Background
The insects are various in types, strong similarity exists among some insects, and effective distinguishing is difficult only by means of color, morphological characteristics and textures, so that harmful insects cannot be controlled in a targeted mode. While the reflectance spectra of different insects usually differ greatly, including the amplitude of the spectrum and the shape of the spectrum.
The existing instrument for measuring the surface spectral reflectance of an object is generally in a contact type, namely a measuring hole is in close contact with the measured object, but the trunk of an insect is softer and irregular in shape, and the contact type measuring instrument is used for measuring, so that on one hand, the insect is easily damaged due to extrusion, on the other hand, the irregular shape of the trunk of the insect can cause that the measuring hole cannot be in close contact with the insect, light leakage during measurement is caused, and further, the measuring result is inaccurate.
Therefore, it is necessary to design a method for indirectly measuring the spectral reflectance of the body surface of the insect, which is simple and convenient to operate, easy to implement and capable of ensuring the measurement accuracy.
Disclosure of Invention
The invention provides a method for indirectly measuring the spectral reflectance of the body surface of an insect, which is simple and convenient to operate, easy to implement and capable of ensuring the measurement precision, and aims to overcome the problems that the conventional contact type instrument for measuring the spectral reflectance of the surface of an object is complex to operate, difficult to implement and inaccurate in measurement result in the prior art.
In order to achieve the purpose, the invention adopts the following technical scheme:
a method for indirectly measuring the spectral reflectance of the surface of an insect body comprises the following steps:
(1-1) measuring the spectral reflectance of the surface of the correction plate by using a spectrophotometer to obtain the spectral reflectance r of the surface of the correction plate;
(1-2) respectively placing a light source at the left side and the right side of the correction plate, and projecting light rays of the two light sources to the surface of the correction plate, wherein the normal lines of the two light sources and the surface of the correction plate are at 45 degrees and have the same distance with the center of the correction plate;
(1-3) placing the spectral radiometer right in front of the correction plate and on the same side with the two light sources, wherein the measurement direction of the spectral radiometer is vertical to the surface of the correction plate, and illumination and measurement geometrical conditions of 45 degrees x:0 degrees are formed between the spectral radiometer and the light sources;
(1-4) measuring the spectral radiance l reflected by the surface of the insect body by using a spectral radiometersAnd the spectral radiance l reflected by the surface of the correction platec
(1-5) calculating the spectral reflectance of the surface of the insect body.
The invention realizes the indirect measurement of the spectral reflectance of the body surface of the insect by utilizing the spectral reflectance of the correction plate and the spectral radiance of the correction plate and the insect. The invention has the characteristics of simple and convenient operation, easy implementation and capability of ensuring the measurement precision.
Preferably, the step (1-3) of the method for indirectly measuring the spectral reflectance on the surface of the insect body further comprises the following steps:
the spectral radiance of the reflection from the insect body surface was measured with a spectral radiometer and recorded as ls(ii) a Keeping the position of the spectral radiometer unchanged, aligning the spectral radiometer with the surface of the correction plate after removing the insects on the surface of the correction plate, measuring the spectral radiance reflected by the surface of the correction plate, and recording the spectral radiance as lc
Preferably, the step (1-4) of the method for indirectly measuring the spectral reflectance on the surface of the insect body further comprises the following steps:
using the formula rs=ls./lcCalculating the spectral reflectance of the insect body surface, wherein rsFor the spectral reflectance of the insect body surface, "/" indicates that the corresponding elements of the vector are divided, and ". dot" indicates that the corresponding elements of the vector are multiplied.
In addition, several measurements were made for each organ or part of each insect tested and the average was taken as spectral reflectance data for one organ or part of each insect.
Preferably, the correction plate is made of matte photographic paper, the model of the spectrophotometer is SP64, the model of the spectral radiometer is CS-2000, and the measurement aperture of the spectral radiometer is 0.2 degrees. The experimental tool with the model can improve the measurement accuracy.
Therefore, the invention has the following beneficial effects: (1) the spectral reflectance of the correction plate and the spectral radiance of the correction plate and the insect are utilized to realize indirect measurement of the spectral reflectance of the body surface of the insect, avoid damaging the insect and ensure the measurement precision; (2) the operation is simple and convenient, and the implementation is easy.
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FIG. 1 is a schematic view of the present invention;
FIG. 2 is a graph of spectral reflectance of an insect body surface as a function of wavelength measured in accordance with the present invention.
In the figure: calibration plate 1, light source 2, spectral radiometer 3, insect 4.
Detailed Description
The invention is further described with reference to the following detailed description and accompanying drawings:
example (b): a method for indirectly measuring the spectral reflectance of an insect body surface as shown in fig. 1, comprising the steps of:
(1-1) measuring the spectral reflectance of the surface of the correction plate 1 by using a spectrophotometer to obtain the spectral reflectance r of the surface of the correction plate;
(1-2) respectively placing a light source 2 on the left side and the right side of the correction plate, and projecting light rays of the two light sources to the surface of the correction plate, wherein the normal lines of the two light sources and the surface of the correction plate are at an angle of 45 degrees and have the same distance with the center of the correction plate;
(1-3) placing the spectral radiometer 3 right in front of the correction plate and on the same side with the two light sources, wherein the measurement direction of the spectral radiometer is vertical to the surface of the correction plate, and illumination and measurement geometrical conditions of 45 degrees x:0 degrees are formed between the spectral radiometer and the light sources;
(1-4) spectral radiance of reflection on the body surface of the insect 4 was measured by a spectral radiometer and recorded as ls(ii) a Keeping the position of the spectral radiometer unchanged, aligning the spectral radiometer with the surface of the correction plate after removing the insects on the surface of the correction plate, measuring the spectral radiance reflected by the surface of the correction plate, and recording the spectral radiance as lc
(1-5) calculating the spectral reflectance of the surface of the insect body: using the formula rs=ls./lcCalculating the spectral reflectance of the insect body surface, wherein rsThe spectral reflectance of the surface of the insect body, "/" indicates that the corresponding elements of the vector are divided, and ". dot" indicates that the corresponding elements of the vector are multiplied; the measurements were repeated 3 times for each organ or part of each insect tested and the average of the 3 measurements was taken as spectral reflectance data for one organ or part of each insect.
The specific measurement results are shown in fig. 2.
In addition, the correction plate adopts matte photographic paper, the model of the spectrophotometer is SP64, the model of the spectral radiometer is CS-2000, and the measurement aperture of the spectral radiometer is 0.2 degrees. The two light sources are fluorescent lamp light sources.
It should be understood that this example is for illustrative purposes only and is not intended to limit the scope of the present invention. Further, it should be understood that various changes or modifications of the present invention may be made by those skilled in the art after reading the teaching of the present invention, and such equivalents may fall within the scope of the present invention as defined in the appended claims.

Claims (4)

1. A method for indirectly measuring the spectral reflectance of the body surface of an insect is characterized by comprising the following steps:
(1-1) measuring the spectral reflectance of the surface of the correction plate (1) by using a spectrophotometer to obtain the spectral reflectance r of the surface of the correction plate;
(1-2) respectively placing a light source (2) at the left side and the right side of the correction plate, and projecting light rays of the two light sources to the surface of the correction plate, wherein the normal lines of the two light sources and the surface of the correction plate are at an angle of 45 degrees and have the same distance with the center of the correction plate;
(1-3) placing the spectral radiometer (3) right in front of the correction plate and on the same side with the two light sources, wherein the measurement direction of the spectral radiometer is perpendicular to the surface of the correction plate, and illumination and measurement geometrical conditions of 45 degrees x:0 degrees are formed between the spectral radiometer and the light sources;
(1-4) measuring the spectral radiance l reflected by the surface of the insect body by using a spectral radiometersAnd the spectral radiance l reflected by the surface of the correction platec(ii) a Measuring each organ or part of each insect in the experiment for a plurality of times, and taking an average value as spectral reflectance data of one organ or part of each insect;
(1-5) calculating the spectral reflectance of the surface of the insect body.
2. The method for indirectly measuring the spectral reflectance on the surface of an insect body according to claim 1, wherein the step (1-3) further comprises the steps of:
the spectral radiance of the reflection from the insect body surface was measured with a spectral radiometer and recorded as ls(ii) a Keeping the position of the spectral radiometer unchanged, aligning the spectral radiometer with the surface of the correction plate after removing the insects on the surface of the correction plate, measuring the spectral radiance reflected by the surface of the correction plate, and recording the spectral radiance as lc
3. The method for indirectly measuring the spectral reflectance on the surface of an insect body according to claim 1, wherein the step (1-4) further comprises the steps of:
using the formula rs= ls./ lcCalculating the spectral reflectance of the insect body surface, wherein rsFor spectral reflectance of insect body surface, "/" indicates that the vector corresponds to the element division, ".Representing the multiplication of corresponding elements of the vector.
4. The method for indirectly measuring the spectral reflectance on the surface of an insect body according to claim 1, 2 or 3, wherein the correction plate is made of matte photographic paper, the spectrophotometer model is SP64, the spectral radiometer model is CS-2000, and the measurement aperture of the spectral radiometer is 0.2 °.
CN201811611762.XA 2018-12-27 2018-12-27 Method for indirectly measuring spectral reflectance of insect body surface Active CN109855734B (en)

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JPH11281325A (en) * 1998-03-27 1999-10-15 Toshiba Corp Image recognition system and image recognition method using the same
US6351308B1 (en) * 1999-11-24 2002-02-26 Xerox Corporation Color printer color control system with automatic spectrophotometer calibration system
CN1769864A (en) * 2004-11-05 2006-05-10 广州大学 Reflective spectrum device
JP2014016243A (en) * 2012-07-09 2014-01-30 Muto Kogyo Kk System and method for diagnosis of spectral photometer
JP6080410B2 (en) * 2012-07-13 2017-02-15 キヤノン株式会社 Spectral colorimeter
CN202748312U (en) * 2012-08-10 2013-02-20 深圳出入境检验检疫局动植物检验检疫技术中心 Test rack for testing reflectance spectrum of insects

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